CA1036935A - Method and apparatus for radioimmunoassay with regeneration of immunadsorbent - Google Patents
Method and apparatus for radioimmunoassay with regeneration of immunadsorbentInfo
- Publication number
- CA1036935A CA1036935A CA194,686A CA194686A CA1036935A CA 1036935 A CA1036935 A CA 1036935A CA 194686 A CA194686 A CA 194686A CA 1036935 A CA1036935 A CA 1036935A
- Authority
- CA
- Canada
- Prior art keywords
- antigen
- immunoadsorbent
- antibodies
- mass
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000003127 radioimmunoassay Methods 0.000 title claims abstract description 13
- 230000008929 regeneration Effects 0.000 title claims description 9
- 238000011069 regeneration method Methods 0.000 title claims description 9
- 239000000427 antigen Substances 0.000 claims abstract description 143
- 102000036639 antigens Human genes 0.000 claims abstract description 142
- 108091007433 antigens Proteins 0.000 claims abstract description 142
- 229920001184 polypeptide Polymers 0.000 claims abstract description 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 4
- 230000003100 immobilizing effect Effects 0.000 claims abstract description 3
- 238000012742 biochemical analysis Methods 0.000 claims abstract 2
- 239000000523 sample Substances 0.000 claims description 44
- 239000002904 solvent Substances 0.000 claims description 43
- 230000001172 regenerating effect Effects 0.000 claims description 18
- 238000004458 analytical method Methods 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 9
- 239000012488 sample solution Substances 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 239000011324 bead Substances 0.000 claims description 6
- 229920002307 Dextran Polymers 0.000 claims description 5
- 230000002411 adverse Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 230000002285 radioactive effect Effects 0.000 claims description 4
- 239000005495 thyroid hormone Substances 0.000 claims description 4
- 229940036555 thyroid hormone Drugs 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- 241000700605 Viruses Species 0.000 claims description 2
- 230000002209 hydrophobic effect Effects 0.000 claims description 2
- 239000000463 material Substances 0.000 claims 4
- 238000012801 analytical assay Methods 0.000 claims 3
- 150000003431 steroids Chemical class 0.000 claims 3
- 239000012857 radioactive material Substances 0.000 claims 1
- 230000000717 retained effect Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 6
- 238000006467 substitution reaction Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 description 11
- 239000000700 radioactive tracer Substances 0.000 description 11
- 238000001514 detection method Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 5
- PROQIPRRNZUXQM-UHFFFAOYSA-N (16alpha,17betaOH)-Estra-1,3,5(10)-triene-3,16,17-triol Natural products OC1=CC=C2C3CCC(C)(C(C(O)C4)O)C4C3CCC2=C1 PROQIPRRNZUXQM-UHFFFAOYSA-N 0.000 description 4
- 229920005654 Sephadex Polymers 0.000 description 4
- 239000012507 Sephadex™ Substances 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 description 4
- 239000003463 adsorbent Substances 0.000 description 4
- PROQIPRRNZUXQM-ZXXIGWHRSA-N estriol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H]([C@H](O)C4)O)[C@@H]4[C@@H]3CCC2=C1 PROQIPRRNZUXQM-ZXXIGWHRSA-N 0.000 description 4
- 229960001348 estriol Drugs 0.000 description 4
- 229960003604 testosterone Drugs 0.000 description 4
- 229910052722 tritium Inorganic materials 0.000 description 4
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 239000003610 charcoal Substances 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- -1 isopropyl alcohols Chemical class 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- CNRNYORZJGVOSY-UHFFFAOYSA-N 2,5-diphenyl-1,3-oxazole Chemical compound C=1N=C(C=2C=CC=CC=2)OC=1C1=CC=CC=C1 CNRNYORZJGVOSY-UHFFFAOYSA-N 0.000 description 1
- VLDFXDUAENINOO-UHFFFAOYSA-N 4-methyl-2-[4-(4-methyl-5-phenyl-1,3-oxazol-2-yl)phenyl]-5-phenyl-1,3-oxazole Chemical compound CC=1N=C(C=2C=CC(=CC=2)C=2OC(=C(C)N=2)C=2C=CC=CC=2)OC=1C1=CC=CC=C1 VLDFXDUAENINOO-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 238000013142 basic testing Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011491 glass wool Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000005258 radioactive decay Effects 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US342513A US3896217A (en) | 1973-03-19 | 1973-03-19 | Method and apparatus for radioimmunoassay with regeneration of immunoadsorbent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1036935A true CA1036935A (en) | 1978-08-22 |
Family
ID=23342159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA194,686A Expired CA1036935A (en) | 1973-03-19 | 1974-03-12 | Method and apparatus for radioimmunoassay with regeneration of immunadsorbent |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JPS5717257B2 (enrdf_load_stackoverflow) |
| CA (1) | CA1036935A (enrdf_load_stackoverflow) |
| IT (1) | IT1018252B (enrdf_load_stackoverflow) |
| ZA (1) | ZA741688B (enrdf_load_stackoverflow) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA761751B (en) * | 1975-04-07 | 1977-03-30 | Summa Corp | Immobilized immunoadsorbent |
| JPS5837482Y2 (ja) * | 1978-04-19 | 1983-08-24 | 共立エコ−物産株式会社 | スプレ−ヤの給水装置 |
| JPS57131062A (en) * | 1981-02-05 | 1982-08-13 | Amano Pharmaceut Co Ltd | Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier |
| AU551103B2 (en) * | 1981-02-13 | 1986-04-17 | Becton Dickinson & Company | Reusable assay for multisite antigens |
| JPS607363A (ja) * | 1983-06-27 | 1985-01-16 | Sekisui Chem Co Ltd | 抗原もしくは抗体の測定方法 |
| CA1230552A (en) * | 1983-11-07 | 1987-12-22 | Howard M. Chandler | Device and method for performing qualitative enzyme immunoassays |
| JPS60125562A (ja) * | 1983-12-09 | 1985-07-04 | Olympus Optical Co Ltd | フロ−インジエクシヨン式分析方法 |
| JPS60162956A (ja) * | 1984-02-04 | 1985-08-24 | Olympus Optical Co Ltd | 免疫学的自動分析方法 |
| JPS62106369A (ja) * | 1985-11-05 | 1987-05-16 | Kanebo Ltd | 免疫測定法 |
-
1974
- 1974-03-12 CA CA194,686A patent/CA1036935A/en not_active Expired
- 1974-03-14 ZA ZA00741688A patent/ZA741688B/xx unknown
- 1974-03-15 IT IT1259774A patent/IT1018252B/it active
- 1974-03-18 JP JP3086674A patent/JPS5717257B2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS49125517A (enrdf_load_stackoverflow) | 1974-12-02 |
| JPS5717257B2 (enrdf_load_stackoverflow) | 1982-04-09 |
| ZA741688B (en) | 1975-03-26 |
| IT1018252B (it) | 1977-09-30 |
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