BRPI0705744A2 - composição de enzimas, uso da composição na hidrólise enzimática de material lignocelulósico, processo de produção de enzimas que degradam a fração de polissacarìdeos da biomassa, processo de produção de álcool utilizando a composição de enzima - Google Patents

composição de enzimas, uso da composição na hidrólise enzimática de material lignocelulósico, processo de produção de enzimas que degradam a fração de polissacarìdeos da biomassa, processo de produção de álcool utilizando a composição de enzima

Info

Publication number
BRPI0705744A2
BRPI0705744A2 BRPI0705744-0A BRPI0705744A BRPI0705744A2 BR PI0705744 A2 BRPI0705744 A2 BR PI0705744A2 BR PI0705744 A BRPI0705744 A BR PI0705744A BR PI0705744 A2 BRPI0705744 A2 BR PI0705744A2
Authority
BR
Brazil
Prior art keywords
enzymes
composition
biomass
cellulolytic
enzyme composition
Prior art date
Application number
BRPI0705744-0A
Other languages
English (en)
Inventor
Elba P Da S Bon
Gottschalk Leda Maria Fortes
Maria Antonieta Ferrara
Eleutherio Elis Cristina Araujo
Pereira Marcos Dias
Ferreira Edivaldo Ximenes Jr
Da Silva Ayla Sant'ana
Sobral Teixeira Ricardo Sposina
De Souza Moreira Leonora Rios
Colin Webb
Original Assignee
Fundacao Universidade De Brasilia
Unicamp
Univ Rio De Janeiro
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundacao Universidade De Brasilia, Unicamp, Univ Rio De Janeiro filed Critical Fundacao Universidade De Brasilia
Priority to BRPI0705744-0A priority Critical patent/BRPI0705744B1/pt
Priority to PCT/BR2008/000344 priority patent/WO2009065199A1/en
Publication of BRPI0705744A2 publication Critical patent/BRPI0705744A2/pt
Publication of BRPI0705744B1 publication Critical patent/BRPI0705744B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01073—Feruloyl esterase (3.1.1.73)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

COMPOSIçãO DE ENZIMAS, USO DA COMPOSIçãO NA HIDRóLISE ENZIMáTICA DE MATERIAL LIGNOCELULÈSICO, PROCESSO DE PRODUçãO DE ENZIMAS QUE DEGRADAM A FRAçãO DE POLISSAC.ARìDEOS DA BIOMASSA, PROCESSO DE PRODUçãO DE áLCOOL UTILIZANDO A COMPOSIçãO DE ENZIMAS. A presente invenção se refere a uma composição de enzimas compreendendo (a) enzimas celulolíticas obtidas por fermentação com Trichoderrna reesei; (b) enzimas celulolíticas, hemicelulolíticas, <225>-glicosidase e enzimas acessórias obtidas por fermentação com Aspergilius awamori; (c) adicionalmente, pelo menos uma espécie molecular selecionada do grupo de enzimas e/ou peptideos com atividade CMCase, ditas espécies moleculares estando presentes nos sobrenadantes do cultivo de T. reesei e/ou A. awamori como apresentado no zimograma da Figura 6; e (d) opcionalmente, um veículo compatível com ditas enzimas, sendo que as ditas enzimas (a) e (b) interagem de modo a resultar em efeito sinérgico dado pela ação das enzimas acessórias que, entre outras atividades, rompem as ligações entre a lignina e os polissacarídeos da biomassa, notadamente a hemicelulose. A presente invenção compreende, ainda, uma composição de enzimas compreendendo: (a) enzimas celuloliticas obtidas por fermentação com Trichoderma .reesei; (b) enzimas celuloliticas, hemicelulolíticas, <225>-glicosidase e enzimas acessórias obtidas por fermentação com Aspergilius awamori; e (c) opcionalmente um veículo compatível com ditas enzimas, sendo que as ditas enzimas (a) e (b) interagem de modo a resultar em efeito sínérgíco dado pela ação das enzimas acessórias e espécies moleculares que atuam de forma diferenciada nos polissacarideos da biomassa e também nas ligações entre a lignina e a hemicelulose. A invenção ainda inclui um processo de produção de ditas enzimas celuloliticas e hemícelulolítícas, <225>- glicosidase e enzimas acessórias e ditas espécies moleculares que compõem a composição da invenção e um processo de produção de álcool a partir de biomassa utilizando, na etapa de hidrólise enzímática, a composição da invenção.
BRPI0705744-0A 2007-11-19 2007-11-19 Composition of enzymes, use of composition in enzymatic hydrolysis of lignocellulose material, process of production of enzymes that degrades the fraction of polymacarides of the biomass, process of alcohol production using enzyme composition BRPI0705744B1 (pt)

Priority Applications (2)

Application Number Priority Date Filing Date Title
BRPI0705744-0A BRPI0705744B1 (pt) 2007-11-19 2007-11-19 Composition of enzymes, use of composition in enzymatic hydrolysis of lignocellulose material, process of production of enzymes that degrades the fraction of polymacarides of the biomass, process of alcohol production using enzyme composition
PCT/BR2008/000344 WO2009065199A1 (en) 2007-11-19 2008-11-19 Enzyme composition from trichoderma reesei and aspergillus awamori

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BRPI0705744-0A BRPI0705744B1 (pt) 2007-11-19 2007-11-19 Composition of enzymes, use of composition in enzymatic hydrolysis of lignocellulose material, process of production of enzymes that degrades the fraction of polymacarides of the biomass, process of alcohol production using enzyme composition

Publications (2)

Publication Number Publication Date
BRPI0705744A2 true BRPI0705744A2 (pt) 2009-07-21
BRPI0705744B1 BRPI0705744B1 (pt) 2017-06-13

Family

ID=40667068

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0705744-0A BRPI0705744B1 (pt) 2007-11-19 2007-11-19 Composition of enzymes, use of composition in enzymatic hydrolysis of lignocellulose material, process of production of enzymes that degrades the fraction of polymacarides of the biomass, process of alcohol production using enzyme composition

Country Status (2)

Country Link
BR (1) BRPI0705744B1 (pt)
WO (1) WO2009065199A1 (pt)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113367160B (zh) * 2021-06-09 2022-07-01 云南星耀生物制品有限公司 绿色木霉菌制剂在防治番茄灰霉病中的应用
CN113667609B (zh) * 2021-08-26 2023-05-12 泸州老窖股份有限公司 水解白酒丢糟的混合菌酶液的制备方法
CN114058605B (zh) * 2021-11-10 2023-05-12 湖北大学 一种提高纤维素酶比活力的方法及其应用
CN116083405B (zh) * 2023-03-07 2023-09-05 中国科学院天津工业生物技术研究所 一种酒糟降解酶制剂及菌酶协同生产单细胞蛋白的方法

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FI89613C (fi) * 1991-12-05 1993-10-25 Valtion Teknillinen Foerfarande foer enzymatisk behandling av cellulosamassor
US7449550B2 (en) * 2003-02-27 2008-11-11 Alliance For Sustainable Energy, Llc Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum

Also Published As

Publication number Publication date
BRPI0705744B1 (pt) 2017-06-13
WO2009065199A1 (en) 2009-05-28

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