BRPI0514571A - meio de rediferenciação para fazer com que condrócitos desdiferenciados se rediferenciem em condrócitos - Google Patents

meio de rediferenciação para fazer com que condrócitos desdiferenciados se rediferenciem em condrócitos

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Publication number
BRPI0514571A
BRPI0514571A BRPI0514571-6A BRPI0514571A BRPI0514571A BR PI0514571 A BRPI0514571 A BR PI0514571A BR PI0514571 A BRPI0514571 A BR PI0514571A BR PI0514571 A BRPI0514571 A BR PI0514571A
Authority
BR
Brazil
Prior art keywords
chondrocytes
redifferentiation
undifferentiated
redifferentiate
condrocites
Prior art date
Application number
BRPI0514571-6A
Other languages
English (en)
Inventor
Kazuto Hoshi
Yuichi Tei
Hiroshi Kawaguchi
Kozo Nakamura
Tsuyoshi Takato
Original Assignee
Fujisoft Inc
Kazuto Hoshi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujisoft Inc, Kazuto Hoshi filed Critical Fujisoft Inc
Publication of BRPI0514571A publication Critical patent/BRPI0514571A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/395—Thyroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

MEIO DE REDIFERENCIAçãO PARA FAZER COM QUE CONDRóCITOS DESDIFERENCIADOS SE REDIFERENCIEM EM CONDRóCITOS. A presente invenção refere-se a um meio de rediferenciação e um método de rediferenciação para fazer com que condrócitos desdiferenciados se rediferenciem em condrócitos originais, os condrócitos desdiferenciados possuindo características de cartilagem atenuadas devido à desdiferenciação durante a cultura in vitro. Portanto, a invenção apresenta um meio de rediferenciação que é usado para rediferenciar os condrócitos desdiferendados em condrócitos originais e contém insulina e pelo menos um selecionado de BMP-2 e análogos dos mesmos. A invenção também apresenta um método para a rediferenciação de condrócitos desdiferenciados em condrócitos por cultivo dos condrócitos desdiferenciados usando o meio de rediferenciação. O meio de rediferenciação também contém, de preferência, T3.
BRPI0514571-6A 2004-08-24 2005-08-23 meio de rediferenciação para fazer com que condrócitos desdiferenciados se rediferenciem em condrócitos BRPI0514571A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004244114 2004-08-24
PCT/JP2005/015280 WO2006022263A1 (ja) 2004-08-24 2005-08-23 脱分化型軟骨細胞の軟骨細胞への再分化用培地

Publications (1)

Publication Number Publication Date
BRPI0514571A true BRPI0514571A (pt) 2008-06-17

Family

ID=35967474

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0514571-6A BRPI0514571A (pt) 2004-08-24 2005-08-23 meio de rediferenciação para fazer com que condrócitos desdiferenciados se rediferenciem em condrócitos

Country Status (9)

Country Link
US (1) US20070134793A1 (pt)
EP (1) EP1792977A4 (pt)
JP (1) JP4469371B2 (pt)
KR (1) KR100900360B1 (pt)
CN (1) CN101014699B (pt)
BR (1) BRPI0514571A (pt)
RU (1) RU2355761C2 (pt)
WO (1) WO2006022263A1 (pt)
ZA (1) ZA200701555B (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2556147B1 (en) 2010-04-08 2018-10-03 The University Court Of The University of Edinburgh Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof
KR101277970B1 (ko) * 2010-06-08 2013-06-27 경희대학교 산학협력단 연골질환 치료용 조성물, 인공연골 및 이들의 제조방법
JP5388233B2 (ja) * 2011-01-19 2014-01-15 富士ソフト株式会社 再生軟骨の軟骨特性を評価する方法
CN102399745A (zh) * 2011-11-22 2012-04-04 浙江大学 一种软骨干细胞分离培养方法
RU2494469C1 (ru) * 2012-04-02 2013-09-27 Федеральное государственное бюджетное учреждение "Новосибирский научно-исследовательский институт травматологии и ортопедии" Министерства здравоохранения и социального развития Российской Федерации (ФГБУ "ННИИТО" Минздравсоцразвития России) Способ коррекции дефекта межпозвонкового диска в эксперименте
CN104368043B (zh) * 2014-09-25 2016-02-24 浙江星月生物科技股份有限公司 一种胶原基质软骨修复膜、制备方法及应用
EP3423071A4 (en) * 2016-03-04 2019-12-04 Lacerta Technologies Inc. chondrocyte
BR112019027313A2 (pt) * 2017-06-21 2020-07-21 Mogrify Limited Métodos para reprogramar uma célula fonte e um condrócito, para gerar uma célula, para prevenir a desdiferenciação de uma cultura de célula decondrócito, para tratar osteoartrite ou outra condição distinguida pela degeneração de tecido cartilaginoso e para identificar um agente útil para promover a conversão de um tipo de célula fonte em um tipo de célula de condrócito, célula, populaçãode células, usos de um ou mais compostos e de uma população de células, um ou mais de ácido [4-[(5,6,7,8-tetra-hidro-5,5,8,8-tetrametil-2-naftalenil)carboxamido]benzoico (am580), ácido retinoico todo trans, ácido retinoico 9-cis, betaestradiol, calcitriol, ciglitazona, cartogenina, cloreto de lítio, melatonina, cloridreto de rhosin, leucovorina, vorinostat e forscolina, composição farmacêutica, kit para uso em um método, e, processos para reprogramar uma célula fonte, para gerar uma célula e para prevenir a desdiferenciação de uma cultura de célula de condrócito
WO2020102715A1 (en) * 2018-11-16 2020-05-22 Rapa Therapeutics, Llc Method for t cell de-differentiation and resulting cells
WO2021254296A1 (zh) * 2020-06-14 2021-12-23 浙江大学 一种生物活性物质组合物、包含所述组合物的无血清培养基及其用途

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0948255T3 (da) * 1996-12-06 2006-03-06 Osiris Therapeutics Inc Forbedret chondrogen differentiering af humane mesenkymale stamceller
ES2251842T3 (es) * 1998-09-18 2006-05-01 Massachusetts Institute Of Technology Uso de factores de crecimiento y hormonas para expansion de celulas de mamiferos e ingenieria de tejidos.
DE10026789B4 (de) * 2000-05-31 2004-09-02 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Knorpelersatz und Verfahren zu dessen Herstellung
US7169610B2 (en) * 2002-01-25 2007-01-30 Genzyme Corporation Serum-free media for chondrocytes and methods of use thereof
WO2003084385A2 (en) * 2002-04-02 2003-10-16 William Marsh Rice University Redifferentiated cells for repairing cartilage defects

Also Published As

Publication number Publication date
RU2007110826A (ru) 2008-10-10
US20070134793A1 (en) 2007-06-14
RU2355761C2 (ru) 2009-05-20
EP1792977A1 (en) 2007-06-06
ZA200701555B (en) 2008-07-30
JPWO2006022263A1 (ja) 2008-05-08
CN101014699B (zh) 2010-05-26
EP1792977A4 (en) 2007-11-21
JP4469371B2 (ja) 2010-05-26
KR20070047789A (ko) 2007-05-07
WO2006022263A1 (ja) 2006-03-02
CN101014699A (zh) 2007-08-08
KR100900360B1 (ko) 2009-06-02

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

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