BRPI0506549B1 - método para avaliação da integridade da cromatina/dna de células de esperma de um animal e kit para sua realização - Google Patents

método para avaliação da integridade da cromatina/dna de células de esperma de um animal e kit para sua realização Download PDF

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Publication number
BRPI0506549B1
BRPI0506549B1 BRPI0506549A BRPI0506549A BRPI0506549B1 BR PI0506549 B1 BRPI0506549 B1 BR PI0506549B1 BR PI0506549 A BRPI0506549 A BR PI0506549A BR PI0506549 A BRPI0506549 A BR PI0506549A BR PI0506549 B1 BRPI0506549 B1 BR PI0506549B1
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BR
Brazil
Prior art keywords
sperm
dna
solution
chromatin
denaturing
Prior art date
Application number
BRPI0506549A
Other languages
English (en)
Portuguese (pt)
Inventor
Jaime Gosalvez Berenguer
Jose Luis Fernandez Garcia
Vicente Goyanes Villaescusa
Original Assignee
Univ Madrid Autonoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Univ Madrid Autonoma filed Critical Univ Madrid Autonoma
Publication of BRPI0506549A publication Critical patent/BRPI0506549A/pt
Publication of BRPI0506549B1 publication Critical patent/BRPI0506549B1/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/137Concentration of a component of medium

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BRPI0506549A 2004-01-26 2005-01-26 método para avaliação da integridade da cromatina/dna de células de esperma de um animal e kit para sua realização BRPI0506549B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200400163 2004-01-26
PCT/IB2005/000187 WO2006079864A1 (es) 2004-01-26 2005-01-26 Un procedimiento para la determinacion de la fragmentacion del and en espermatozoides de animales

Publications (2)

Publication Number Publication Date
BRPI0506549A BRPI0506549A (pt) 2007-02-27
BRPI0506549B1 true BRPI0506549B1 (pt) 2016-02-16

Family

ID=34965581

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0506549A BRPI0506549B1 (pt) 2004-01-26 2005-01-26 método para avaliação da integridade da cromatina/dna de células de esperma de um animal e kit para sua realização

Country Status (19)

Country Link
US (1) US7993827B2 (enExample)
EP (1) EP1710320B1 (enExample)
JP (1) JP4773423B2 (enExample)
KR (1) KR20070090073A (enExample)
CN (1) CN1934272A (enExample)
AT (1) ATE454473T1 (enExample)
AU (1) AU2005320348B2 (enExample)
BR (1) BRPI0506549B1 (enExample)
CA (1) CA2554409C (enExample)
CY (1) CY1109866T1 (enExample)
DE (1) DE602005018742D1 (enExample)
DK (1) DK1710320T3 (enExample)
ES (1) ES2337592T3 (enExample)
IL (1) IL176897A0 (enExample)
PL (1) PL1710320T3 (enExample)
PT (1) PT1710320E (enExample)
RU (1) RU2373288C2 (enExample)
SI (1) SI1710320T1 (enExample)
WO (1) WO2006079864A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2329637B1 (es) * 2006-11-10 2010-09-22 Universidad Autonoma De Madrid Procedimiento para la determinacion de la fragmentacion del adn en microorganismos.
RU2437100C1 (ru) * 2010-05-18 2011-12-20 Государственное учреждение Научно-исследовательский институт физико-химической биологии имени А.Н. Белозерского Московского государственного университета имени М.В. Ломоносова Способ диагностики аномалий упаковки хроматина сперматозоидов при мужском бесплодии
ES2396820B1 (es) * 2011-07-26 2014-01-31 Universidad Autónoma de Madrid Método para evaluar la integridad de la pared celular bacteriana.
US8818734B1 (en) 2011-11-18 2014-08-26 Dagan Wells Peptide ligands for sperm DNA fragmentation assay
EP2927326B1 (en) 2014-03-31 2018-11-07 FertiPro N.V. Method and tools for determining DNA fragmentation
CN108182676B (zh) * 2017-12-22 2021-11-02 成都朴华科技有限公司 一种精子碎片化率检测方法、装置、设备及可读存储介质
CN109115735B (zh) * 2018-07-27 2020-01-17 浙江博真生物科技有限公司 精子染色质结构检测试剂盒及其应用
CN112301097B (zh) * 2019-07-26 2022-10-21 申翌生物科技(杭州)有限公司 样本裂解和pcr反应组合物
WO2021000750A1 (zh) 2019-07-01 2021-01-07 申翌生物科技(杭州)有限公司 利用pcr综合反应系统进行pcr反应的新方法
CN111307696A (zh) * 2020-03-19 2020-06-19 浙江星博生物科技股份有限公司 用于检测精子dna碎片率的方法和试剂盒
LT4019645T (lt) 2020-12-23 2023-10-10 Bonraybio Co., Ltd. Spermos dnr fragmentacijos nustatymo metodai ir rinkiniai
LT4019646T (lt) * 2020-12-23 2025-08-25 Bonraybio Co., Ltd. Spermos dnr fragmentacijos nustatymo metodai ir rinkiniai
TWI775252B (zh) * 2020-12-23 2022-08-21 邦睿生技股份有限公司 用於偵測精子dna片段化的方法以及套組
CN114088491A (zh) * 2021-11-24 2022-02-25 北京仁基源医学研究院有限公司 一种精子dna碎片检测试剂盒及其检测方法
CN117030411A (zh) * 2023-08-10 2023-11-10 深圳市盛信康科技有限公司 一种精子核dna完整性检测试剂盒

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801058A (en) * 1994-06-20 1998-09-01 Blanco-Esteve; Carmen Analytic method to determine the fertilizing capacity of a human semen sample
RU2099024C1 (ru) * 1995-02-07 1997-12-20 Всероссийский научно-исследовательский институт животноводства Способ оценки биологической полноценности сперматозоидов

Also Published As

Publication number Publication date
AU2005320348A8 (en) 2008-12-18
EP1710320B1 (en) 2010-01-06
JP4773423B2 (ja) 2011-09-14
SI1710320T1 (sl) 2010-07-30
BRPI0506549A (pt) 2007-02-27
CA2554409C (en) 2018-03-27
US7993827B2 (en) 2011-08-09
IL176897A0 (en) 2008-03-20
EP1710320A1 (en) 2006-10-11
ES2337592T3 (es) 2010-04-27
RU2006130740A (ru) 2008-03-10
PT1710320E (pt) 2010-03-04
HK1096430A1 (en) 2007-06-01
AU2005320348B2 (en) 2010-06-24
JP2007521840A (ja) 2007-08-09
DE602005018742D1 (de) 2010-02-25
WO2006079864A1 (es) 2006-08-03
CN1934272A (zh) 2007-03-21
PL1710320T3 (pl) 2010-06-30
CA2554409A1 (en) 2006-08-03
AU2005320348A1 (en) 2006-08-17
CY1109866T1 (el) 2014-09-10
US20070281298A1 (en) 2007-12-06
RU2373288C2 (ru) 2009-11-20
KR20070090073A (ko) 2007-09-05
ATE454473T1 (de) 2010-01-15
DK1710320T3 (da) 2010-05-03

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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