BRPI0411975A - mutantes da acetohidróxi ácido sintetase resistentes à retroalimentação - Google Patents

mutantes da acetohidróxi ácido sintetase resistentes à retroalimentação

Info

Publication number
BRPI0411975A
BRPI0411975A BRPI0411975-4A BRPI0411975A BRPI0411975A BR PI0411975 A BRPI0411975 A BR PI0411975A BR PI0411975 A BRPI0411975 A BR PI0411975A BR PI0411975 A BRPI0411975 A BR PI0411975A
Authority
BR
Brazil
Prior art keywords
nucleic acid
polypeptide
sequence
acid sequences
cited above
Prior art date
Application number
BRPI0411975-4A
Other languages
English (en)
Inventor
Miroslav Patek
Veronika Elisakova
Original Assignee
Degussa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa filed Critical Degussa
Publication of BRPI0411975A publication Critical patent/BRPI0411975A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

Abstract

"MUTANTES DA ACETOHIDRóXI áCIDO SINTETASE RESISTENTES à RETROALIMENTAçãO". A presente invenção refere-se a seqüências de nucleotídeos que codificam mutantes da acetohidróxi ácido sintetase (AHAS), as próprias enzimas mutadas e um processo para a produção fermentativa de aminoácidos de cadeia ramificada utilizando estas enzimas em hospedeiros específicos em que os genes que codificam a acetohidróxi ácido sintetase (AHAS) mutante são expressos.
BRPI0411975-4A 2003-06-26 2004-06-08 mutantes da acetohidróxi ácido sintetase resistentes à retroalimentação BRPI0411975A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20030014640 EP1491634A1 (en) 2003-06-26 2003-06-26 Feedback resistant acetohydroxy acid synthetase mutants
PCT/EP2004/006157 WO2005003357A1 (en) 2003-06-26 2004-06-08 Feedback resistant acetohydroxy acid synthethase mutants

Publications (1)

Publication Number Publication Date
BRPI0411975A true BRPI0411975A (pt) 2006-08-29

Family

ID=33395884

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0411975-4A BRPI0411975A (pt) 2003-06-26 2004-06-08 mutantes da acetohidróxi ácido sintetase resistentes à retroalimentação

Country Status (11)

Country Link
US (2) US7585959B2 (pt)
EP (2) EP1491634A1 (pt)
JP (1) JP2007506407A (pt)
KR (1) KR20060024437A (pt)
CN (1) CN100523197C (pt)
AT (1) ATE450611T1 (pt)
BR (1) BRPI0411975A (pt)
DE (1) DE602004024394D1 (pt)
RU (1) RU2325439C2 (pt)
WO (1) WO2005003357A1 (pt)
ZA (1) ZA200510448B (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2355763C2 (ru) * 2006-09-13 2009-05-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Мутантная ацетолактатсинтаза и способ продукции разветвленных l-аминокислот
WO2012014228A1 (en) 2010-07-28 2012-02-02 Abhishek Narain Singh A method to by-pass allosteric domain activity of an enzyme so as to alter its feedback or feed-forward inhibition or activation
DE102011118019A1 (de) 2011-06-28 2013-01-03 Evonik Degussa Gmbh Varianten des Promotors des für die Glyzerinaldehyd-3-phosphat-Dehydrogenase kodierenden gap-Gens
DK2811028T3 (en) 2013-06-03 2017-05-01 Evonik Degussa Gmbh Process for Preparation of L-Valine Using Recombinant Coryn Bacteria Containing the Propionate Inducible IlvBN Operon
KR101720836B1 (ko) 2014-08-05 2017-04-03 씨제이제일제당 (주) 피드백 저항성 아세토하이드록시산 신타아제 변이체 및 이를 이용한 l-발린의 생산방법
CN105886431B (zh) * 2016-04-27 2019-05-10 天津科技大学 一株谷氨酸棒状杆菌及其高产异亮氨酸的方法
WO2017194696A1 (en) 2016-05-12 2017-11-16 Danmarks Tekniske Universitet Bacterial cells with improved tolerance to isobutyric acid
US11021697B2 (en) 2017-07-11 2021-06-01 Cj Cheiljedang Corporation Acetohydroxy acid synthase variant, microorganism comprising the same, and method of producing L-branched-chain amino acid using the same
KR101996129B1 (ko) 2017-07-11 2019-07-04 씨제이제일제당 (주) 아세토하이드록시산 신타아제 변이체, 이를 포함하는 미생물 또는 이를 이용하는 l-분지쇄 아미노산 생산 방법
CA3119088A1 (en) * 2018-12-12 2020-06-18 Utilization Of Carbon Dioxide Institute Co., Ltd. A recombinant hydrogenophilus bacterium with enhanced ability to produce valine
CN110229797B (zh) * 2019-06-05 2020-08-28 天津科技大学 一种乙酰羟酸合成酶及其应用
KR102147381B1 (ko) * 2019-11-22 2020-08-24 씨제이제일제당 주식회사 아세토하이드록시산 신타제 신규 변이체 및 이를 포함하는 미생물
KR20230045990A (ko) * 2021-09-29 2023-04-05 씨제이제일제당 (주) 신규한 아세토하이드록시산 신테아제 변이체 및 이를 이용한 l-이소류신 생산방법
KR20230045989A (ko) * 2021-09-29 2023-04-05 씨제이제일제당 (주) 신규한 아세토하이드록시산 신테아제 변이체 및 이를 이용한 l-이소류신 생산방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2748418B2 (ja) * 1988-08-03 1998-05-06 味の素株式会社 組換えｄｎａ、該組換えｄｎａを有する微生物
DE3942947A1 (de) * 1989-12-23 1991-06-27 Forschungszentrum Juelich Gmbh Verfahren zur herstellung von l-isoleucin und dafuer geeignete mikroorganismen und rekombinante dna
JP4623825B2 (ja) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
RU2209246C2 (ru) * 2000-01-26 2003-07-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Малая субъединица изозима iii и изозим iii синтетазы ацетогидроксикислот из escherichia coli, фрагмент днк (варианты), штамм бактерии escherichia coli - продуцент l-валина (варианты) и способ получения l-валина
ES2234912T3 (es) * 2000-10-13 2005-07-01 Archer-Daniels-Midland Company Gen de piruvato carboxilasa resistente a la retroalimentacion de corynebacterium.

Also Published As

Publication number Publication date
CN100523197C (zh) 2009-08-05
ATE450611T1 (de) 2009-12-15
JP2007506407A (ja) 2007-03-22
EP1636366B1 (en) 2009-12-02
RU2006102024A (ru) 2006-09-10
US20070292914A1 (en) 2007-12-20
WO2005003357A1 (en) 2005-01-13
EP1636366A1 (en) 2006-03-22
US20100086966A1 (en) 2010-04-08
US7585959B2 (en) 2009-09-08
CN1813065A (zh) 2006-08-02
DE602004024394D1 (en) 2010-01-14
EP1491634A1 (en) 2004-12-29
KR20060024437A (ko) 2006-03-16
RU2325439C2 (ru) 2008-05-27
ZA200510448B (en) 2006-12-27

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

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