BR9909643A - Processo de produção de uma proteìna recombinante de interesse, construção primeira utilizada para transformar uma célula hospedeira procariótica suscetìvel de ser transformada por uma segunda construção, vetor e célula hospedeira - Google Patents
Processo de produção de uma proteìna recombinante de interesse, construção primeira utilizada para transformar uma célula hospedeira procariótica suscetìvel de ser transformada por uma segunda construção, vetor e célula hospedeiraInfo
- Publication number
- BR9909643A BR9909643A BR9909643-9A BR9909643A BR9909643A BR 9909643 A BR9909643 A BR 9909643A BR 9909643 A BR9909643 A BR 9909643A BR 9909643 A BR9909643 A BR 9909643A
- Authority
- BR
- Brazil
- Prior art keywords
- host cell
- construction
- recombinant protein
- interest
- transformed
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/32011—Picornaviridae
- C12N2770/32611—Poliovirus
- C12N2770/32622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
"PROCESSO DE PRODUçãO DE UMA PROTEìNA RECOMBINANTE DE INTERESSE, CONSTRUçãO PRIMEIRA UTILIZADA PARA TRANSFORMAR UMA CéLULA HOSPEDEIRA PROCARIóTICA SUSCETìVEL DE SER TRANSFORMADA POR UMA SEGUNDA CONSTRUçãO, VETOR E CéLULA HOSPEDEIRA". A invenção compreende uma nova construção para a expressão de um gene codificando para uma proteína recombinante de interesse colocada sob o controle do promotor do óperon triptofano Ptrp em uma célula hospedeira procariótica, caracterizada pelo fato de que a construção compreende uma seq³ência nucleica capaz de inativar o gene codificando para uma triptofanase TnaA quando a referida seq³ência nucleica é introduzida na referida célula hospedeira, vetores contendo a referida construção e as células hospedeiras transformadas pelos referidos vetores. A invenção também tem por objeto os processos de produção das referidas proteínas recombinantes utilizando estas novas construções.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9804638A FR2777292B1 (fr) | 1998-04-14 | 1998-04-14 | Nouvelles constructions pour l'expression controlee de proteines recombinantes dans des cellules procaryotes |
PCT/FR1999/000874 WO1999053080A1 (fr) | 1998-04-14 | 1999-04-14 | Nouvelles constructions pour l'expression controlee de proteines recombinantes dans des cellules procaryotes |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9909643A true BR9909643A (pt) | 2000-12-26 |
Family
ID=9525222
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9909643-9A BR9909643A (pt) | 1998-04-14 | 1999-04-14 | Processo de produção de uma proteìna recombinante de interesse, construção primeira utilizada para transformar uma célula hospedeira procariótica suscetìvel de ser transformada por uma segunda construção, vetor e célula hospedeira |
Country Status (14)
Country | Link |
---|---|
US (1) | US6844169B1 (pt) |
EP (1) | EP1071798B9 (pt) |
JP (1) | JP2002511273A (pt) |
CN (1) | CN1261581C (pt) |
AT (1) | ATE316579T1 (pt) |
AU (1) | AU768595B2 (pt) |
BR (1) | BR9909643A (pt) |
CA (1) | CA2325601A1 (pt) |
DE (1) | DE69929590T2 (pt) |
DK (1) | DK1071798T3 (pt) |
ES (1) | ES2257853T3 (pt) |
FR (1) | FR2777292B1 (pt) |
PT (1) | PT1071798E (pt) |
WO (1) | WO1999053080A1 (pt) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050249667A1 (en) * | 2004-03-24 | 2005-11-10 | Tuszynski Jack A | Process for treating a biological organism |
US11485977B2 (en) * | 2014-12-19 | 2022-11-01 | Adagene, Inc. | Methods and systems for autoinduction of protein expression |
CN106676125B (zh) * | 2015-11-05 | 2020-08-14 | 中国科学院天津工业生物技术研究所 | 一种包含麦芽糖启动子的载体以及麦芽糖启动子突变体 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0293207A3 (en) * | 1987-05-29 | 1989-11-02 | The Standard Oil Company | Eschericia coli carrying recombinant plasmid for the production of tryptophan |
DE8709371U1 (de) | 1987-07-08 | 1987-08-20 | Alcoa Deutschland Gmbh Verpackungswerke, 6520 Worms | Flaschenschraubverschluß mit Fingerschürze |
US5416008A (en) * | 1991-10-01 | 1995-05-16 | California Institute Of Technology | Cross-regulation of gene expression in recombinant cells |
-
1998
- 1998-04-14 FR FR9804638A patent/FR2777292B1/fr not_active Expired - Fee Related
-
1999
- 1999-04-14 ES ES99913408T patent/ES2257853T3/es not_active Expired - Lifetime
- 1999-04-14 DE DE69929590T patent/DE69929590T2/de not_active Expired - Lifetime
- 1999-04-14 BR BR9909643-9A patent/BR9909643A/pt not_active Application Discontinuation
- 1999-04-14 CN CN99806686.9A patent/CN1261581C/zh not_active Expired - Fee Related
- 1999-04-14 WO PCT/FR1999/000874 patent/WO1999053080A1/fr active IP Right Grant
- 1999-04-14 JP JP2000543627A patent/JP2002511273A/ja active Pending
- 1999-04-14 EP EP99913408A patent/EP1071798B9/fr not_active Expired - Lifetime
- 1999-04-14 US US09/673,288 patent/US6844169B1/en not_active Expired - Fee Related
- 1999-04-14 CA CA002325601A patent/CA2325601A1/fr not_active Abandoned
- 1999-04-14 DK DK99913408T patent/DK1071798T3/da active
- 1999-04-14 PT PT99913408T patent/PT1071798E/pt unknown
- 1999-04-14 AT AT99913408T patent/ATE316579T1/de not_active IP Right Cessation
- 1999-04-14 AU AU31540/99A patent/AU768595B2/en not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
FR2777292A1 (fr) | 1999-10-15 |
EP1071798B9 (fr) | 2007-06-13 |
CA2325601A1 (fr) | 1999-10-21 |
FR2777292B1 (fr) | 2000-07-07 |
EP1071798B1 (fr) | 2006-01-25 |
DK1071798T3 (da) | 2006-05-15 |
AU3154099A (en) | 1999-11-01 |
CN1303439A (zh) | 2001-07-11 |
ATE316579T1 (de) | 2006-02-15 |
JP2002511273A (ja) | 2002-04-16 |
WO1999053080A1 (fr) | 1999-10-21 |
DE69929590D1 (de) | 2006-04-13 |
US6844169B1 (en) | 2005-01-18 |
AU768595B2 (en) | 2003-12-18 |
EP1071798A1 (fr) | 2001-01-31 |
DE69929590T2 (de) | 2006-09-07 |
CN1261581C (zh) | 2006-06-28 |
PT1071798E (pt) | 2006-05-31 |
ES2257853T3 (es) | 2006-08-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O E 25 DA LPI. |
|
B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL. |