BR9907248A - Determinação de hidreto usando compostos de acridìnio quimiluminescentes - Google Patents

Determinação de hidreto usando compostos de acridìnio quimiluminescentes

Info

Publication number
BR9907248A
BR9907248A BR9907248-3A BR9907248A BR9907248A BR 9907248 A BR9907248 A BR 9907248A BR 9907248 A BR9907248 A BR 9907248A BR 9907248 A BR9907248 A BR 9907248A
Authority
BR
Brazil
Prior art keywords
hydride
source
acridinium
nadh
compound
Prior art date
Application number
BR9907248-3A
Other languages
English (en)
Inventor
David Sharpe
Anand Natrajan
Qingping Jiang
George Parsons
Say-Jong Law
Original Assignee
Bayer Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag filed Critical Bayer Ag
Publication of BR9907248A publication Critical patent/BR9907248A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Patente de Invenção: <B>"DETERMINAçãO DE HIDRETO USANDO COMPOSTOS DE ACRIDìNIO QUIMILUMINESCENTES"<D>. A presente invenção divulga um método para a determinação de hidreto, usando um composto quimiluminescente, tal como um composto de acridínio. A fonte de hidreto para a redução do composto de acridínio pode ser de origem química ou bioquímica ou o resultado de catálise enzimática. A fonte química do hidreto pode ser NaBH~ 4~. Uma fonte bioquímica de hidreto pode ser uma fonte derivada de NADH ou NADPH, enquanto uma fonte enzimática seria a classe das oxidorredutases, denominada de desidrogenase, que converte NADH ou NADPH de NAD ou NADP. Entre as aplicações dos compostos de acridínio como indicadores quimiluminescentes de hidreto, se encontram os ensaios de diagnósticos usando desidrogenases como reagentes, indicadores, marcadores de diagnósticos ou como marcadores. O etanol, por exemplo, pode ser detectado através de quimiluminescência de éster de acridínio, mediante reação de desidrogenase de álcool em etanol, dita reação produzindo NADH. Como marcador, a desidrogenase pode ser usada em um procedimento ELISA para detecção de um analisado específico, com o éster de acridínio proporcionando a resposta de sinalização.
BR9907248-3A 1998-11-25 1999-11-24 Determinação de hidreto usando compostos de acridìnio quimiluminescentes BR9907248A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10982398P 1998-11-25 1998-11-25
PCT/IB1999/001894 WO2000031543A1 (en) 1998-11-25 1999-11-24 Measurement of hydride using chemiluminescent acridinium compounds

Publications (1)

Publication Number Publication Date
BR9907248A true BR9907248A (pt) 2000-10-17

Family

ID=22329751

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9907248-3A BR9907248A (pt) 1998-11-25 1999-11-24 Determinação de hidreto usando compostos de acridìnio quimiluminescentes

Country Status (10)

Country Link
EP (1) EP1049933B1 (pt)
JP (2) JP2002530678A (pt)
AT (1) ATE304711T1 (pt)
AU (1) AU1173400A (pt)
BR (1) BR9907248A (pt)
CA (1) CA2319187A1 (pt)
DE (1) DE69927240T2 (pt)
ES (1) ES2249065T3 (pt)
IL (1) IL136929A0 (pt)
WO (1) WO2000031543A1 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6573043B1 (en) 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor
US6127140A (en) * 1999-06-18 2000-10-03 Abbott Laboratories Assay for quantitative measurement of analytes in biological samples
DE10051220A1 (de) * 2000-10-16 2002-04-25 Mettler Toledo Gmbh Optischer Sensor zur Bestimmung eines Analyten und Verfahren zu dessen Herstellung
US6664043B2 (en) * 2001-07-03 2003-12-16 Bayer Corporation Acridinium ester labels having hydrophilic modifiers
WO2004018418A2 (en) * 2002-08-20 2004-03-04 Quest Diagnostics Investments Incorporated Hydrophilic chemiluminescent acridinium labeling reagents
US6924154B2 (en) 2002-08-20 2005-08-02 Quest Diagnostics Investments Incorporated Hydrophilic chemilumescent acridinium labeling reagents
US7319041B2 (en) * 2002-09-27 2008-01-15 Siemens Medical Solutions Diagnostic Applications of acridinium compounds and derivatives in homogeneous assays
WO2005103676A1 (ja) * 2004-04-23 2005-11-03 Japan Science And Technology Agency 水素分布の可視化方法、水素分布の可視化システム、水素分布の可視化用発光試薬、水素検出方法および水素検出試薬
US7906293B2 (en) * 2007-04-09 2011-03-15 Abbott Laboratories Acridinium phenyl esters useful in the analysis of biological
JP6270127B2 (ja) * 2014-01-17 2018-01-31 国立研究開発法人物質・材料研究機構 ニコチンアデニンジヌクレオチド誘導体を検出する方法
EP3183246B1 (en) 2014-08-22 2020-09-23 Roche Diagnostics GmbH Redoxindicators

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE111083T1 (de) * 1986-10-22 1994-09-15 Abbott Lab Chemilumineszierende acridinium- und phenantridiniumsalze.
US5656426A (en) * 1988-08-01 1997-08-12 Chiron Diagnostics Corporation Functionaized hydrophilic acridinium esters

Also Published As

Publication number Publication date
DE69927240D1 (de) 2005-10-20
IL136929A0 (en) 2001-06-14
JP2002530678A (ja) 2002-09-17
CA2319187A1 (en) 2000-06-02
ES2249065T3 (es) 2006-03-16
JP2010237226A (ja) 2010-10-21
WO2000031543A1 (en) 2000-06-02
EP1049933B1 (en) 2005-09-14
AU1173400A (en) 2000-06-13
EP1049933A1 (en) 2000-11-08
DE69927240T2 (de) 2006-06-22
ATE304711T1 (de) 2005-09-15

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Legal Events

Date Code Title Description
FA10 Dismissal: dismissal - article 33 of industrial property law
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]