KR960704063A - 질산염 또는 아질산염이온 감지용 바이오센서(biosensors for detecting nitrate or nitrite ions) - Google Patents

질산염 또는 아질산염이온 감지용 바이오센서(biosensors for detecting nitrate or nitrite ions)

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Publication number
KR960704063A
KR960704063A KR1019960700323A KR19960700323A KR960704063A KR 960704063 A KR960704063 A KR 960704063A KR 1019960700323 A KR1019960700323 A KR 1019960700323A KR 19960700323 A KR19960700323 A KR 19960700323A KR 960704063 A KR960704063 A KR 960704063A
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enzyme
biosensor
nitrate
nitrite
reaction
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KR1019960700323A
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English (en)
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지오프레이 윌리암 그랜햄
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디알 스코츠
브리티쉬 누클레이 퓨엘스 피엘씨
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Publication of KR960704063A publication Critical patent/KR960704063A/ko

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/004—Enzyme electrodes mediator-assisted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90209—Oxidoreductases (1.) acting on NADH or NADPH (1.6), e.g. those with a heme protein as acceptor (1.6.2) (general), Cytochrome-b5 reductase (1.6.2.2) or NADPH-cytochrome P450 reductase (1.6.2.4)

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

바이오센서는 질산염 또는 아질산염이온 존재하에 생화학 반응을 생성할 수 있는 생화학물질과 질산염 또는 아질산염이온 존재하에 생화학물질에 의해 생산된 반응으로 직간접적으로 생산된 변환성 출력물을 감지할 수 있도록 배열된 변환기로 구성되고 이때, 생화학물질은 질산염 또는 아질산염이온의 환원효소와 질산염 또는 아질산염의 환원에 의해 산화될 수 있는 공-인자로 구성되고 생화학물질은 공-효소의 산화에 의해 직간접적으로 광자를 생산하는 광발광 성분을 포함하고 변환기는 광감지기로 구성된다.

Description

질산염 또는 아질산염이온 감지용 바이오센서(BIOSENSORS FOR DETECTING NITRATE OR NITRITE IONS)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명에 따른 바이오센서 배열의 측면도이고,
제2도는 본 발명에 따른 또 다른 바이오센서의 측면도이고,
제3도는 본 발명에 따라 구체화된 바이오센서에서 이용되는 생화학반응 관련 일반 과정을 도식적으로 나타낸 것이고,
제5도는 본 발명에 따라 구체화된 바이오센서에서 이용되는 생화학 과정의 예를 도식적으로 나타낸 것이다.

Claims (8)

  1. 바이오센서는 질산염 또는 아질산염이온 존재하에 생화학 반응을 생성할 수 있는 생화학물질과 질산염 또는 아질산염이온 존재하에 생화학물질에 의해 생산된 반응으로 직간접적으로 생산된 변환성 출력물을 감지할 수 있도록 배열된 변환기로 구성되고 이때, 생화학물질은 질산염 또는 아질산염이온의 환원 효소와 질산염 또는 아질산염의 환원에 의해 산화될 수 있는 공-인자로 구성되고 생화학물질은 공-효소의 산화에 의해 직간접적으로 광자를 생산하는 광발광 성분을 포함하고 변환기는 광감지기로 구성된 것을 특징으로 하는 바이오센서.
  2. 제1항에 있어서, 고정된 효소 조성물은 질산염 또는 아질산염 환원효소 일정량과 산화된 상태와 환원된 상태를 가지는 공-인자 일정량과 산화상태의 공인자하에 산화반응을 유도할 수 있는 공-효소 일정량으로 구성되고 이때 공-인자는 환원효소에 의한 환원반응으로 환원상태에서 산화상태로 전환할 수 있고, 따라서 공-인자는 환원효소에 의해 추가 환원반응을 허용하기 위해 환원상태의 공인자의 공급을 유지하기 위해 공인자는 환원상태로 전환하게 되고. 추가로 제2공-효소에 의해 유도되는 산화반응 정도와, 산화된 상태에 있는 공-인자의 형성을 측정하는 제2공-효소에 의한 산환정도를 측정하기 위한 수단이 포함되어 환원 효소에 의해 환원된 질산염 또는 아질산염의 양을 측정하게 되고, 감지수단은 제2공-효소에 의해 유도된 산화반응의 결과로써 직간접적으로 방출되는 광자를 감지하기 위한 광감지기로 구성된 것을 특징으로 하는 바이오센서.
  3. 제2항에 있어서, 바이오센서는 질산염 환원반응에 의해 생산된 아질산염이 아질산염 환원효소에 의해 추가 환원될 수 있도록 질산염 공-효소와 아질산염 공-효소 모두가 결합된 고정된 효소 조성물을 포함하는 것을 특징으로 하는 바이오센서.
  4. 제1항 내지 3항 중 어느 한 항에 있어서, 산화될 공-인자는 환원효소에 의해 제공되는 환원반응과 같이 NADP+로 산화될 니코틴아미드 효소 NADPH로 구성된 것을 특징으로 하는 바이오센서.
  5. 제1항 내지 4항중 어느 한 항에 있어서, 광자는 공-효소 화학반응에서 작용하는 하나이상의 다른 공-효소를 경유하여 생산되는 것을 특징으로 하는 바이오센서.
  6. 제1항 내지 5항중 어느 한 항에 있어서, 광자는 NADPH와 함께 생화학반응에서 이용된 효소 루시퍼라제에 의해 생산되는 것을 특징으로 하는 바이오센서.
  7. 제2항 내지 6항중 어느 한 항에 있어서. NADP+의 생산은 알코을 디하이드로게나제의 존재하에 유기알코올이 이에 상응하는 알데히드로의 산화반응에 의해 감지되는 것을 특징으로 하는 바이오센서.
  8. 제7항에 있어서, 알데히드는 반응을 촉매하는 효소 루시퍼라제와 함께 산소존재하에 환원된 플라빈 FMNH2와의 반응에 의해 감지되는 것을 특징으로 하는 바이오센서.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960700323A 1994-05-28 1995-05-30 질산염 또는 아질산염이온 감지용 바이오센서(biosensors for detecting nitrate or nitrite ions) KR960704063A (ko)

Applications Claiming Priority (3)

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GB9410805A GB9410805D0 (en) 1994-05-28 1994-05-28 Biosensors
GB9410805.7 1994-05-28
PCT/GB1995/001235 WO1995033068A1 (en) 1994-05-28 1995-05-30 Biosensors for detecting nitrate or nitrite ions

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KR960704063A true KR960704063A (ko) 1996-08-31

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US (1) US5776715A (ko)
EP (1) EP0711357A1 (ko)
JP (1) JPH09501573A (ko)
KR (1) KR960704063A (ko)
AU (1) AU695040B2 (ko)
GB (1) GB9410805D0 (ko)
RU (1) RU2149182C1 (ko)
WO (1) WO1995033068A1 (ko)

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EP0837941B1 (en) * 1995-07-11 2003-03-12 The Secretary of State for Defence Detection and biodegradation of explosives
GB2317388B (en) * 1995-07-11 1999-11-03 Secr Defence Detection and biodegradation of explosives
DK172855B1 (da) * 1996-11-12 1999-08-16 Unisense Aps Fremgangsmåde til måling af koncentrationen af et medium i et miljø, mikrosensor til brug ved fremgangsmåden og anvendelse
WO2005119237A1 (en) * 2004-05-24 2005-12-15 Samsung Engineering Co., Ltd. Biosensor for detecting nitrate ion and measuring method using the same
US7727206B2 (en) * 2005-12-27 2010-06-01 Gorres Geoffrey H Device for monitoring a patient for a urinary tract infection
US20100120073A1 (en) * 2007-05-08 2010-05-13 Superior Medical Llc Methods and devices for detecting organisms causing urinary tract infections
US9187779B2 (en) 2011-02-22 2015-11-17 The Nitrate Elimination Co., Inc. Systems and methods for enzymatic oxygen removal
CN103088113A (zh) * 2011-11-03 2013-05-08 中国科学院生态环境研究中心 一种检测硝酸盐的微生物细胞传感器
US20150291628A1 (en) 2013-01-28 2015-10-15 Council Of Scientific & Industrial Research Novel aza bodipy compound for the selective detection of nitrite ions in water and a process for preparation thereof
CN105651842B (zh) * 2016-01-12 2018-11-09 扬州大学 一种花瓣状聚苯胺硫化钼复合物、制备及其应用
CN105758913B (zh) * 2016-05-16 2018-09-14 扬州大学 一种秸秆纤维素-二硫化钼复合物修饰电极的制备方法
CN114894871B (zh) * 2022-05-16 2024-01-16 安徽大学 一种高灵敏度亚硝酸还原酶生物电极的制备方法及应用

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US4791055A (en) * 1978-04-10 1988-12-13 Miles Inc. Homogenous specific binding assay reagent system and labeled conjugates
US4321123A (en) * 1978-04-21 1982-03-23 Matsushita Electric Industrial Co., Ltd. Coenzyme immobilized electrode
FR2637912B1 (fr) * 1988-10-14 1991-01-11 Univ Paris Optrodes a enzymes immobilises et a substances luminescentes, portables et automatisees, pour les dosages des effecteurs de la chaine respiratoire, des oxydases et des deshydrogenases, et procedes de dosages correspondants
JPH06104230B2 (ja) * 1992-06-01 1994-12-21 正和 黒田 生体触媒固定化電極及び同電極を用いた水処理方法
GB9315183D0 (en) * 1993-07-22 1993-09-08 British Nuclear Fuels Plc Biosensors

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JPH09501573A (ja) 1997-02-18
WO1995033068A1 (en) 1995-12-07
GB9410805D0 (en) 1994-07-20
US5776715A (en) 1998-07-07
EP0711357A1 (en) 1996-05-15
AU2677095A (en) 1995-12-21
RU2149182C1 (ru) 2000-05-20
AU695040B2 (en) 1998-08-06

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