BR112023027256A2 - Método para amplificar isotermicamente uma sequência alvo de ácidos nucleicos - Google Patents
Método para amplificar isotermicamente uma sequência alvo de ácidos nucleicosInfo
- Publication number
- BR112023027256A2 BR112023027256A2 BR112023027256A BR112023027256A BR112023027256A2 BR 112023027256 A2 BR112023027256 A2 BR 112023027256A2 BR 112023027256 A BR112023027256 A BR 112023027256A BR 112023027256 A BR112023027256 A BR 112023027256A BR 112023027256 A2 BR112023027256 A2 BR 112023027256A2
- Authority
- BR
- Brazil
- Prior art keywords
- probe
- primers
- reaction
- detection
- stranded dna
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 239000000523 sample Substances 0.000 abstract 6
- 108020004414 DNA Proteins 0.000 abstract 4
- 102000053602 DNA Human genes 0.000 abstract 4
- 238000006243 chemical reaction Methods 0.000 abstract 4
- 238000001514 detection method Methods 0.000 abstract 4
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 2
- 230000027455 binding Effects 0.000 abstract 2
- 102000007260 Deoxyribonuclease I Human genes 0.000 abstract 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000006073 displacement reaction Methods 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000011901 isothermal amplification Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 238000012123 point-of-care testing Methods 0.000 abstract 1
- 230000009870 specific binding Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110733555.7A CN113481283A (zh) | 2021-06-30 | 2021-06-30 | 等温扩增核酸靶序列的方法 |
| PCT/CN2022/102545 WO2023274330A1 (fr) | 2021-06-30 | 2022-06-29 | Procédé d'amplification isotherme de séquences cibles d'acides nucléiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112023027256A2 true BR112023027256A2 (pt) | 2024-03-12 |
Family
ID=77937009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112023027256A BR112023027256A2 (pt) | 2021-06-30 | 2022-06-29 | Método para amplificar isotermicamente uma sequência alvo de ácidos nucleicos |
Country Status (4)
| Country | Link |
|---|---|
| CN (2) | CN113481283A (fr) |
| AU (1) | AU2022301095A1 (fr) |
| BR (1) | BR112023027256A2 (fr) |
| WO (1) | WO2023274330A1 (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113481283A (zh) * | 2021-06-30 | 2021-10-08 | 上海伯杰医疗科技有限公司北京分公司 | 等温扩增核酸靶序列的方法 |
| CN113981047B (zh) * | 2021-11-08 | 2023-11-07 | 中国科学院合肥物质科学研究院 | 一种用于miRNA检测的逆转录-链置换扩增方法及应用 |
| CN114350756A (zh) * | 2021-11-22 | 2022-04-15 | 西安交通大学 | 基于dna切刻/聚合链置换循环反应的全基因组自引发扩增方法及试剂盒 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10590474B2 (en) * | 2013-03-11 | 2020-03-17 | Elitechgroup B.V. | Methods for true isothermal strand displacement amplification |
| CN104726549B (zh) * | 2014-10-10 | 2020-01-21 | 青岛耐德生物技术有限公司 | 一种基于切刻酶的双链核酸等温扩增检测新方法 |
| CN108642144B (zh) * | 2018-05-18 | 2020-06-09 | 贠红岩 | 一种恒温链置换扩增技术及试剂盒 |
| CN111850100B (zh) * | 2020-07-21 | 2022-12-13 | 北京艾克伦医疗科技有限公司 | 核酸扩增方法及其应用 |
| CN113481283A (zh) * | 2021-06-30 | 2021-10-08 | 上海伯杰医疗科技有限公司北京分公司 | 等温扩增核酸靶序列的方法 |
-
2021
- 2021-06-30 CN CN202110733555.7A patent/CN113481283A/zh active Pending
-
2022
- 2022-06-29 CN CN202210751117.8A patent/CN115074419A/zh active Pending
- 2022-06-29 BR BR112023027256A patent/BR112023027256A2/pt unknown
- 2022-06-29 AU AU2022301095A patent/AU2022301095A1/en active Pending
- 2022-06-29 WO PCT/CN2022/102545 patent/WO2023274330A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023274330A1 (fr) | 2023-01-05 |
| AU2022301095A1 (en) | 2024-01-18 |
| CN113481283A (zh) | 2021-10-08 |
| CN115074419A (zh) | 2022-09-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112023027256A2 (pt) | Método para amplificar isotermicamente uma sequência alvo de ácidos nucleicos | |
| Zhang et al. | A protocol for detection of COVID-19 using CRISPR diagnostics | |
| Adams | A beginner’s guide to RT-PCR, qPCR and RT-qPCR | |
| Wang et al. | CRISPR/Cas12a-based dual amplified biosensing system for sensitive and rapid detection of polynucleotide kinase/phosphatase | |
| Liang et al. | Rapid detection and tracking of Omicron variant of SARS-CoV-2 using CRISPR-Cas12a-based assay | |
| BR112022023512A2 (pt) | Ensaio baseado em crispr para a detecção de patógenos em amostras. | |
| Chi et al. | CRISPR-Cas14a-integrated strand displacement amplification for rapid and isothermal detection of cholangiocarcinoma associated circulating microRNAs | |
| CN105349683B (zh) | 目标物保护的哑铃分子探针介导的级联滚环扩增策略用于dna甲基转移酶活性的灵敏检测 | |
| CN107389646B (zh) | 一种检测转录因子NF-κBp50的荧光化学传感器及其检测方法 | |
| Wang et al. | CRISPR-Cas13a cascade-based viral RNA assay for detecting SARS-CoV-2 and its mutations in clinical samples | |
| Nimsamer et al. | Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens | |
| Chen et al. | A universal platform for one-pot detection of circulating non-coding RNA combining CRISPR-Cas12a and branched rolling circle amplification | |
| Cai et al. | An ultrasensitive biosensing platform for FEN1 activity detection based on target-induced primer extension to trigger the collateral cleavage of CRISPR/Cas12a | |
| Tan et al. | Absolute quantification of SARS-CoV-2 with Clarity Plus™ digital PCR | |
| Wei et al. | Label-free and homogeneous detection of flap endonuclease 1 by ligation-promoted hyperbranched rolling circle amplification platform | |
| Chen et al. | Toehold-mediated ligation-free rolling circle amplification enables sensitive and rapid imaging of messenger RNAs in situ in cells | |
| Fan et al. | Development of a visual multiplex fluorescent LAMP assay for the detection of foot-and-mouth disease, vesicular stomatitis and bluetongue viruses | |
| Lai et al. | Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP) | |
| Zhang et al. | An ultra-sensitive and specific UCBiosensor via CRISPR-Cas12a and UDG-mediated polymerase chain reaction | |
| CN104120174B (zh) | 多个microRNAs复合检测的引物设计方法 | |
| Lech et al. | Expression profiling by real-time quantitative polymerase chain reaction (RT-qPCR) | |
| Wang et al. | Development of a cleaved probe-based loop-mediated isothermal amplification assay for rapid detection of African swine fever virus | |
| Pang et al. | Visual detection of CaMV35S promoter via target-triggered rolling circle amplification of DNAzyme | |
| Li et al. | One-pot, ultrasensitive, and multiplex detection of SARS-CoV-2 genes utilizing self-priming hairpin-mediated isothermal amplification | |
| CN106480210A (zh) | 一种肿瘤循环dna甲基化检测引物 |