BR112022019713A2 - Composições que compreendem um polipeptídeo variante cas12i2 e usos das mesmas - Google Patents

Composições que compreendem um polipeptídeo variante cas12i2 e usos das mesmas

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Publication number
BR112022019713A2
BR112022019713A2 BR112022019713A BR112022019713A BR112022019713A2 BR 112022019713 A2 BR112022019713 A2 BR 112022019713A2 BR 112022019713 A BR112022019713 A BR 112022019713A BR 112022019713 A BR112022019713 A BR 112022019713A BR 112022019713 A2 BR112022019713 A2 BR 112022019713A2
Authority
BR
Brazil
Prior art keywords
cas12i2
variant
polypeptides
compositions
complexes
Prior art date
Application number
BR112022019713A
Other languages
English (en)
Inventor
Chong Shaorong
Jay Hilbert Brendan
Norman Wessells Quinton
Michael Jakimo Noah
Ziblat Roy
Michael Carte Jason
Marie Ditommaso Tia
Raymond Haswell Jeffrey
James Garrity Anthony
Alexander Mcgaw Colin
A Scott David
Michael Cerchione Derek
Original Assignee
Arbor Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arbor Biotechnologies Inc filed Critical Arbor Biotechnologies Inc
Publication of BR112022019713A2 publication Critical patent/BR112022019713A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

COMPOSIÇÕES QUE COMPREENDEM UM POLIPEPTÍDEO VARIANTE CAS12I2 E USOS DAS MESMAS. A presente invenção refere-se a polipeptídeos Cas12i2 variantes, métodos para produzir os polipeptídeos Cas12i2 variantes, processos para caracterizar os polipeptídeos Cas12i2 variantes, células que compreendem os polipeptídeos Cas12i2 variantes e métodos para usar os polipeptídeos Cas12i2 variantes. A invenção refere-se, ainda, a complexos que compreendem os polipeptídeos Cas12i2 variantes, métodos para produzir os complexos, processos para caracterizar os complexos, células que compreendem os complexos e métodos para usar os complexos.
BR112022019713A 2020-03-31 2021-03-31 Composições que compreendem um polipeptídeo variante cas12i2 e usos das mesmas BR112022019713A2 (pt)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US202063002999P 2020-03-31 2020-03-31
US202063003090P 2020-03-31 2020-03-31
US202063003064P 2020-03-31 2020-03-31
US202063012007P 2020-04-17 2020-04-17
US202063026277P 2020-05-18 2020-05-18
US202063026606P 2020-05-18 2020-05-18
US202063026523P 2020-05-18 2020-05-18
US202063026562P 2020-05-18 2020-05-18
US202063063879P 2020-08-10 2020-08-10
US202063063819P 2020-08-10 2020-08-10
US202063063859P 2020-08-10 2020-08-10
US202063063831P 2020-08-10 2020-08-10
US202063085792P 2020-09-30 2020-09-30
US202063085752P 2020-09-30 2020-09-30
US202063085862P 2020-09-30 2020-09-30
US202063085895P 2020-09-30 2020-09-30
US202163147968P 2021-02-10 2021-02-10
US202163147850P 2021-02-10 2021-02-10
PCT/US2021/025257 WO2021202800A1 (en) 2020-03-31 2021-03-31 Compositions comprising a cas12i2 variant polypeptide and uses thereof

Publications (1)

Publication Number Publication Date
BR112022019713A2 true BR112022019713A2 (pt) 2022-12-20

Family

ID=75640000

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022019713A BR112022019713A2 (pt) 2020-03-31 2021-03-31 Composições que compreendem um polipeptídeo variante cas12i2 e usos das mesmas

Country Status (12)

Country Link
US (1) US20230332119A1 (pt)
EP (1) EP4127154A1 (pt)
JP (1) JP2023520504A (pt)
KR (1) KR20230009379A (pt)
CN (1) CN115698278A (pt)
AU (1) AU2021249119A1 (pt)
BR (1) BR112022019713A2 (pt)
CA (1) CA3177749A1 (pt)
CO (1) CO2022015170A2 (pt)
IL (1) IL296791A (pt)
MX (1) MX2022012189A (pt)
WO (1) WO2021202800A1 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230193243A1 (en) * 2020-05-29 2023-06-22 Arbor Biotechnologies, Inc. Compositions comprising a cas12i2 polypeptide and uses thereof
KR20230107265A (ko) * 2020-10-30 2023-07-14 아버 바이오테크놀로지스, 인크. Pdcd1을 표적화하는 rna 가이드를 포함하는 조성물 및 이의 용도
JP2024520528A (ja) 2021-06-01 2024-05-24 アーバー バイオテクノロジーズ， インコーポレイテッド Ｃｒｉｓｐｒヌクレアーゼを含む遺伝子編集システム及びそれらの使用
US20230034581A1 (en) 2021-06-04 2023-02-02 Arbor Biotechnologies, Inc. Gene editing systems comprising an rna guide targeting lactate dehydrogenase a (ldha) and uses thereof
IL308803A (en) 2021-06-04 2024-01-01 Arbor Biotechnologies Inc Gene editing systems comprising guide RNA targeting hydroxy acid oxidase 1 (HAO1) and uses thereof
EP4347831A2 (en) 2021-06-04 2024-04-10 Arbor Biotechnologies, Inc. Gene editing systems comprising an rna guide targeting transthyretin (ttr) and uses thereof
KR20240052763A (ko) 2021-08-11 2024-04-23 아버 바이오테크놀로지스, 인크. 스타스민 2 (stmn2)를 표적화하는 rna 가이드를 포함하는 유전자 편집 시스템 및 이의 용도
WO2023018856A1 (en) 2021-08-11 2023-02-16 Arbor Biotechnologies, Inc. Gene editing systems comprising an rna guide targeting polypyrimidine tract binding protein 1 (ptbp1) and uses thereof
WO2023034475A1 (en) 2021-09-01 2023-03-09 Arbor Biotechnologies, Inc. Cells modified by a cas12i polypeptide
TW202334421A (zh) * 2021-11-05 2023-09-01 美商阿伯生物技術公司 包含靶向ciita之rna引導之組合物及其用途
WO2023122433A1 (en) 2021-12-22 2023-06-29 Arbor Biotechnologies, Inc. Gene editing systems targeting hydroxyacid oxidase 1 (hao1) and lactate dehydrogenase a (ldha)
WO2023138685A1 (en) * 2022-01-24 2023-07-27 Huidagene Therapeutics Co., Ltd. Novel crispr-cas12i systems and uses thereof
CN117460822B (zh) * 2022-04-25 2024-08-02 辉大基因治疗(新加坡)私人有限公司 新型CRISPR-Cas12i系统及其用途
WO2024118747A1 (en) 2022-11-30 2024-06-06 Arbor Biotechnologies, Inc. Reverse transcriptase-mediated genetic editing of transthyretin (ttr) and uses thereof
WO2024173645A1 (en) 2023-02-15 2024-08-22 Arbor Biotechnologies, Inc. Gene editing method for inhibiting aberrant splicing in stathmin 2 (stmn2) transcript

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201504523UA (en) 2012-12-12 2015-07-30 Broad Inst Inc Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
CA2930015A1 (en) 2013-11-07 2015-05-14 Editas Medicine, Inc. Crispr-related methods and compositions with governing grnas
DE202019005567U1 (de) * 2018-03-14 2021-02-16 Arbor Biotechnologies, Inc. Neue CRISPR-DNA-Targeting-Enzyme und -Systeme

Also Published As

Publication number Publication date
CN115698278A (zh) 2023-02-03
WO2021202800A1 (en) 2021-10-07
KR20230009379A (ko) 2023-01-17
AU2021249119A1 (en) 2022-10-20
JP2023520504A (ja) 2023-05-17
CO2022015170A2 (es) 2022-11-08
US20230332119A1 (en) 2023-10-19
MX2022012189A (es) 2023-01-05
IL296791A (en) 2022-11-01
CA3177749A1 (en) 2021-10-07
EP4127154A1 (en) 2023-02-08

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