MX2022012189A - Composiciones que comprenden una variante del polipéptido cas12i2 y usos de las mismas. - Google Patents

Composiciones que comprenden una variante del polipéptido cas12i2 y usos de las mismas.

Info

Publication number
MX2022012189A
MX2022012189A MX2022012189A MX2022012189A MX2022012189A MX 2022012189 A MX2022012189 A MX 2022012189A MX 2022012189 A MX2022012189 A MX 2022012189A MX 2022012189 A MX2022012189 A MX 2022012189A MX 2022012189 A MX2022012189 A MX 2022012189A
Authority
MX
Mexico
Prior art keywords
cas12i2
variant
polypeptides
compositions
variant polypeptide
Prior art date
Application number
MX2022012189A
Other languages
English (en)
Inventor
David A Scott
Shaorong Chong
Brendan Jay Hilbert
Quinton Norman Wessells
Noah Michael Jakimo
Roy Ziblat
Jason Michael Carte
Tia Marie Ditommaso
Jeffrey Raymond Haswell
Anthony James Garrity
Colin Alexander Mcgaw
Derek Michael Cerchione
Original Assignee
Arbor Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arbor Biotechnologies Inc filed Critical Arbor Biotechnologies Inc
Publication of MX2022012189A publication Critical patent/MX2022012189A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a variantes del polipéptido Cas12i2, métodos para producir las variantes del polipéptido Cas12i2, procesos para caracterizar las variantes del polipéptido Cas12i2, células que comprenden las variantes del polipéptido Cas12i2 y métodos para utilizar las variantes del polipéptido Cas12i2. La invención se refiere además a complejos que comprenden las variantes del polipéptido Cas12i2, métodos para producir los complejos, procesos para caracterizar los complejos, células que comprenden los complejos y métodos para utilizar de los complejos.
MX2022012189A 2020-03-31 2021-03-31 Composiciones que comprenden una variante del polipéptido cas12i2 y usos de las mismas. MX2022012189A (es)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US202063003064P 2020-03-31 2020-03-31
US202063002999P 2020-03-31 2020-03-31
US202063003090P 2020-03-31 2020-03-31
US202063012007P 2020-04-17 2020-04-17
US202063026562P 2020-05-18 2020-05-18
US202063026277P 2020-05-18 2020-05-18
US202063026606P 2020-05-18 2020-05-18
US202063026523P 2020-05-18 2020-05-18
US202063063831P 2020-08-10 2020-08-10
US202063063859P 2020-08-10 2020-08-10
US202063063819P 2020-08-10 2020-08-10
US202063063879P 2020-08-10 2020-08-10
US202063085862P 2020-09-30 2020-09-30
US202063085895P 2020-09-30 2020-09-30
US202063085792P 2020-09-30 2020-09-30
US202063085752P 2020-09-30 2020-09-30
US202163147850P 2021-02-10 2021-02-10
US202163147968P 2021-02-10 2021-02-10
PCT/US2021/025257 WO2021202800A1 (en) 2020-03-31 2021-03-31 Compositions comprising a cas12i2 variant polypeptide and uses thereof

Publications (1)

Publication Number Publication Date
MX2022012189A true MX2022012189A (es) 2023-01-05

Family

ID=75640000

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022012189A MX2022012189A (es) 2020-03-31 2021-03-31 Composiciones que comprenden una variante del polipéptido cas12i2 y usos de las mismas.

Country Status (12)

Country Link
US (1) US20230332119A1 (es)
EP (1) EP4127154A1 (es)
JP (1) JP2023520504A (es)
KR (1) KR20230009379A (es)
CN (1) CN115698278A (es)
AU (1) AU2021249119A1 (es)
BR (1) BR112022019713A2 (es)
CA (1) CA3177749A1 (es)
CO (1) CO2022015170A2 (es)
IL (1) IL296791A (es)
MX (1) MX2022012189A (es)
WO (1) WO2021202800A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4158020A2 (en) * 2020-05-29 2023-04-05 Arbor Biotechnologies, Inc. Compositions comprising a cas12i2 polypeptide and uses thereof
KR20230107265A (ko) * 2020-10-30 2023-07-14 아버 바이오테크놀로지스, 인크. Pdcd1을 표적화하는 rna 가이드를 포함하는 조성물 및 이의 용도
US20240102007A1 (en) 2021-06-01 2024-03-28 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof
EP4347833A2 (en) 2021-06-04 2024-04-10 Arbor Biotechnologies, Inc. Gene editing systems comprising an rna guide targeting hydroxyacid oxidase 1 (hao1) and uses thereof
US20220396782A1 (en) 2021-06-04 2022-12-15 Arbor Biotechnologies, Inc. Gene editing systems comprising an rna guide targeting transthyretin (ttr) and uses thereof
WO2022256655A2 (en) * 2021-06-04 2022-12-08 Arbor Biotechnologies, Inc. Gene editing systems comprising an rna guide targeting lactate dehydrogenase a (ldha) and uses thereof
US20230203539A1 (en) 2021-08-11 2023-06-29 Arbor Biotechnologies, Inc. Gene editing systems comprising an rna guide targeting stathmin 2 (stmn2) and uses thereof
WO2023018856A1 (en) 2021-08-11 2023-02-16 Arbor Biotechnologies, Inc. Gene editing systems comprising an rna guide targeting polypyrimidine tract binding protein 1 (ptbp1) and uses thereof
WO2023034475A1 (en) 2021-09-01 2023-03-09 Arbor Biotechnologies, Inc. Cells modified by a cas12i polypeptide
TW202334421A (zh) * 2021-11-05 2023-09-01 美商阿伯生物技術公司 包含靶向ciita之rna引導之組合物及其用途
WO2023122433A1 (en) 2021-12-22 2023-06-29 Arbor Biotechnologies, Inc. Gene editing systems targeting hydroxyacid oxidase 1 (hao1) and lactate dehydrogenase a (ldha)
WO2023138685A1 (en) * 2022-01-24 2023-07-27 Huidagene Therapeutics Co., Ltd. Novel crispr-cas12i systems and uses thereof
CN117460822A (zh) * 2022-04-25 2024-01-26 辉大基因治疗(新加坡)私人有限公司 新型CRISPR-Cas12i系统及其用途

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL293526A (en) 2012-12-12 2022-08-01 Harvard College Providing, engineering and optimizing systems, methods and compositions for sequence manipulation and therapeutic applications
US9834791B2 (en) 2013-11-07 2017-12-05 Editas Medicine, Inc. CRISPR-related methods and compositions with governing gRNAS
PT3765615T (pt) * 2018-03-14 2023-08-28 Arbor Biotechnologies Inc Novas enzimas e sistemas de direcionamento de dna crispr

Also Published As

Publication number Publication date
WO2021202800A1 (en) 2021-10-07
CO2022015170A2 (es) 2022-11-08
AU2021249119A1 (en) 2022-10-20
US20230332119A1 (en) 2023-10-19
JP2023520504A (ja) 2023-05-17
KR20230009379A (ko) 2023-01-17
CN115698278A (zh) 2023-02-03
EP4127154A1 (en) 2023-02-08
BR112022019713A2 (pt) 2022-12-20
CA3177749A1 (en) 2021-10-07
IL296791A (en) 2022-11-01

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