BR112022015055A2 - Três reatores de resina em série para sintetizador de peptídeo - Google Patents

Três reatores de resina em série para sintetizador de peptídeo

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Publication number
BR112022015055A2
BR112022015055A2 BR112022015055A BR112022015055A BR112022015055A2 BR 112022015055 A2 BR112022015055 A2 BR 112022015055A2 BR 112022015055 A BR112022015055 A BR 112022015055A BR 112022015055 A BR112022015055 A BR 112022015055A BR 112022015055 A2 BR112022015055 A2 BR 112022015055A2
Authority
BR
Brazil
Prior art keywords
reactors
series
added
reactor
peptide synthesis
Prior art date
Application number
BR112022015055A
Other languages
English (en)
Inventor
D Johnson Martin
E Kopach Michael
P Webster Luke
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of BR112022015055A2 publication Critical patent/BR112022015055A2/pt

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/045—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers using devices to improve synthesis, e.g. reactors, special vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00693—Means for quality control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00698—Measurement and control of process parameters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00707—Processes involving means for analysing and characterising the products separated from the reactor apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00759—Purification of compounds synthesised
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)

Abstract

TRÊS REATORES DE RESINA EM SÉRIE PARA SINTETIZADOR DE PEPTÍDEOA presente invenção refere-se a um dispositivo de Síntese de Peptídeos em Fase Sólida (SPPS) e a um método de uso do mesmo para a fabricação de peptídeos que são ensinados nesta invenção. O sistema compreende pelo menos dois reatores, cada reator incluindo uma quantidade de resina SPPS. Os reatores são posicionados em série. Um agente de desproteção é adicionado ao primeiro reator e depois transferido para os segundo e terceiro reatores, em série, operando assim para desproteger o grupo N protegido. O solvente de lavagem é adicionado ao primeiro reator e depois transferido para o segundo e esta operação é repetida várias vezes. Da mesma forma, uma solução de éster ativado por aminoácido é adicionada, em série, ao primeiro, segundo e terceiro reatores, operando assim para acoplar o aminoácido ao grupo N desprotegido. O solvente de lavagem é adicionado ao primeiro reator e então transferido para o segundo e esta operação é repetida várias vezes antes do próximo ciclo. O uso dos reatores em série reduz o solvente total necessário. A LCMS online também é utilizada para monitorar o progresso e a identidade das reações que acontecem dentro das partículas de resina em fase sólida.
BR112022015055A 2020-02-05 2021-01-29 Três reatores de resina em série para sintetizador de peptídeo BR112022015055A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062970247P 2020-02-05 2020-02-05
PCT/US2021/015856 WO2021158444A2 (en) 2020-02-05 2021-01-29 Three resin reactors in series peptide synthesizer

Publications (1)

Publication Number Publication Date
BR112022015055A2 true BR112022015055A2 (pt) 2022-09-20

Family

ID=74798025

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022015055A BR112022015055A2 (pt) 2020-02-05 2021-01-29 Três reatores de resina em série para sintetizador de peptídeo

Country Status (11)

Country Link
US (1) US20230158468A1 (pt)
EP (1) EP4100417A2 (pt)
JP (2) JP7415022B2 (pt)
KR (1) KR20220137705A (pt)
CN (1) CN115298193A (pt)
AU (1) AU2021216548A1 (pt)
BR (1) BR112022015055A2 (pt)
CA (2) CA3167047A1 (pt)
IL (1) IL295109A (pt)
MX (1) MX2022009598A (pt)
WO (1) WO2021158444A2 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3204051A1 (en) 2021-01-20 2022-07-28 Brian Lian Compositions and methods for the treatment of metabolic and liver disorders
WO2023068987A1 (en) * 2021-10-18 2023-04-27 Polypeptide Laboratories Holding (Ppl) Ab Intermittent percolation washing
WO2023089594A1 (en) 2021-11-22 2023-05-25 Sun Pharmaceutical Industries Limited Process for the preparation of tirzepatide or pharmaceutically acceptable salt thereof
WO2023158599A1 (en) 2022-02-15 2023-08-24 Eli Lilly And Company Solid phase peptide synthesis wash process
WO2024077149A2 (en) * 2022-10-05 2024-04-11 Eli Lilly And Company Peptides for incretin synthesis

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4362699A (en) * 1981-03-10 1982-12-07 Bio Research, Inc. Apparatus for high pressure peptide synthesis
DE3631662A1 (de) * 1986-09-17 1988-03-24 Biotechnolog Forschung Gmbh Verfahren zur simultanen synthese mehrerer peptide an fester phase
ES2245505T3 (es) 1998-03-23 2006-01-01 Trimeris Inc. Metodos y composiciones para la sintesis de peptidos (t-20).
US20020019013A1 (en) * 1999-03-08 2002-02-14 Boliang Lou Combined resin method for high-speed synthesis of combinatorial libraries
EP2204225A1 (en) * 2008-12-22 2010-07-07 SynChro GmbH Column reactor
TWI510781B (zh) * 2010-10-29 2015-12-01 Scinopharm Taiwan Ltd 即時監測固相胜肽合成反應之質譜系統

Also Published As

Publication number Publication date
KR20220137705A (ko) 2022-10-12
CN115298193A (zh) 2022-11-04
JP2024045149A (ja) 2024-04-02
CA3167047A1 (en) 2021-08-12
JP2023512772A (ja) 2023-03-29
IL295109A (en) 2022-09-01
WO2021158444A3 (en) 2021-11-04
EP4100417A2 (en) 2022-12-14
JP7415022B2 (ja) 2024-01-16
US20230158468A1 (en) 2023-05-25
WO2021158444A2 (en) 2021-08-12
MX2022009598A (es) 2022-10-21
AU2021216548A1 (en) 2022-08-25
CA3218467A1 (en) 2021-08-12

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