BR112022007151A2 - Produção de bioproduto em uma célula hospedeira - Google Patents

Produção de bioproduto em uma célula hospedeira

Info

Publication number
BR112022007151A2
BR112022007151A2 BR112022007151A BR112022007151A BR112022007151A2 BR 112022007151 A2 BR112022007151 A2 BR 112022007151A2 BR 112022007151 A BR112022007151 A BR 112022007151A BR 112022007151 A BR112022007151 A BR 112022007151A BR 112022007151 A2 BR112022007151 A2 BR 112022007151A2
Authority
BR
Brazil
Prior art keywords
genetically modified
host cell
cell
bioproduct
bioproduct production
Prior art date
Application number
BR112022007151A
Other languages
English (en)
Inventor
Beauprez Joeri
Coussement Pieter
LANNOO Nausicaä
Peters Gert
Vandewalle Kristof
Vercauteren Annelies
AESAERT Sofie
DECOENE Thomas
Original Assignee
Inbiose Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inbiose Nv filed Critical Inbiose Nv
Publication of BR112022007151A2 publication Critical patent/BR112022007151A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PRODUÇÃO DE BIOPRODUTO EM UMA CÉLULA HOSPEDEIRA. A presente invenção descreve um método de produção de bioprodutos por fermentação com uma célula geneticamente modificada, bem como a célula geneticamente modificada utilizada no método. A célula é geneticamente modificada para produzir um bioproduto e é ainda geneticamente modificada por redução da expressão de pelo menos um gene codificador da proteína da membrana endógena e/ou mutação da expressão da proteína da membrana endógena.
BR112022007151A 2019-10-14 2020-10-14 Produção de bioproduto em uma célula hospedeira BR112022007151A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19202978 2019-10-14
PCT/EP2020/078830 WO2021074182A1 (en) 2019-10-14 2020-10-14 Production of bioproduct in a host cell

Publications (1)

Publication Number Publication Date
BR112022007151A2 true BR112022007151A2 (pt) 2022-08-23

Family

ID=68424573

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022007151A BR112022007151A2 (pt) 2019-10-14 2020-10-14 Produção de bioproduto em uma célula hospedeira

Country Status (9)

Country Link
US (1) US20240076704A1 (pt)
EP (1) EP4045518A1 (pt)
JP (1) JP2022551195A (pt)
KR (1) KR20220080178A (pt)
CN (1) CN114555780A (pt)
AU (1) AU2020366551A1 (pt)
BR (1) BR112022007151A2 (pt)
CA (1) CA3157881A1 (pt)
WO (1) WO2021074182A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024020530A1 (en) * 2022-07-22 2024-01-25 Debut Biotechnology, Inc. Udp-sugar bioproduction using microorganism hosts

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7250274B2 (en) * 2002-12-13 2007-07-31 Zymogenetics, Inc. IL-21 production in prokaryotic hosts
WO2005087940A1 (en) * 2004-03-11 2005-09-22 Wisconsin Alumni Research Foundation Genetically altered microorganisms with modified metabolism
WO2007119891A1 (en) * 2006-04-18 2007-10-25 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE fhuA GENE
RU2366703C2 (ru) * 2007-02-07 2009-09-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПОЛУЧЕНИЯ L-ТРЕОНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia, В КОТОРОЙ ИНАКТИВИРОВАН ГЕН tolC
WO2010142305A1 (en) * 2009-06-08 2010-12-16 Jennewein Biotechnologie Gmbh Hmo synthesis
KR101608734B1 (ko) * 2014-03-21 2016-04-04 씨제이제일제당 주식회사 L-아미노산을 생산하는 미생물 및 이를 이용하여 l-아미노산을 생산하는 방법

Also Published As

Publication number Publication date
WO2021074182A1 (en) 2021-04-22
CN114555780A (zh) 2022-05-27
US20240076704A1 (en) 2024-03-07
AU2020366551A1 (en) 2022-06-02
CA3157881A1 (en) 2021-04-22
JP2022551195A (ja) 2022-12-07
EP4045518A1 (en) 2022-08-24
KR20220080178A (ko) 2022-06-14

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]