BR112022004453A2 - Retroelemento bacteriano modificado com produção intensificada de dna - Google Patents
Retroelemento bacteriano modificado com produção intensificada de dnaInfo
- Publication number
- BR112022004453A2 BR112022004453A2 BR112022004453A BR112022004453A BR112022004453A2 BR 112022004453 A2 BR112022004453 A2 BR 112022004453A2 BR 112022004453 A BR112022004453 A BR 112022004453A BR 112022004453 A BR112022004453 A BR 112022004453A BR 112022004453 A2 BR112022004453 A2 BR 112022004453A2
- Authority
- BR
- Brazil
- Prior art keywords
- retroelement
- retrons
- engineered
- modified bacterial
- dna production
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962899625P | 2019-09-12 | 2019-09-12 | |
| PCT/US2020/050323 WO2021050822A1 (en) | 2019-09-12 | 2020-09-11 | Modified bacterial retroelement with enhanced dna production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112022004453A2 true BR112022004453A2 (pt) | 2022-06-21 |
Family
ID=74865645
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112022004453A BR112022004453A2 (pt) | 2019-09-12 | 2020-09-11 | Retroelemento bacteriano modificado com produção intensificada de dna |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US20220307007A1 (https=) |
| EP (1) | EP4028512A4 (https=) |
| JP (2) | JP2022548062A (https=) |
| KR (1) | KR20220081988A (https=) |
| CN (2) | CN119859624A (https=) |
| AU (1) | AU2020346880B2 (https=) |
| BR (1) | BR112022004453A2 (https=) |
| CA (1) | CA3154384A1 (https=) |
| IL (1) | IL291128A (https=) |
| MX (1) | MX2022003091A (https=) |
| WO (1) | WO2021050822A1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20240263173A1 (en) * | 2021-08-11 | 2024-08-08 | The Board Of Trustees Of The Leland Stanford Junior University | High-throughput precision genome editing in human cells |
| CN118511225A (zh) * | 2021-09-02 | 2024-08-16 | 华盛顿大学 | 多重时间分辨分子信号记录器及相关方法 |
| CA3237482A1 (en) * | 2021-11-03 | 2023-05-11 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Precise genome editing using retrons |
| US11866728B2 (en) | 2022-01-21 | 2024-01-09 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
| EP4499822A1 (en) * | 2022-03-25 | 2025-02-05 | The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone | Rt-dna fidelity and retron genome editing |
| WO2023183627A1 (en) * | 2022-03-25 | 2023-09-28 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Production of reverse transcribed dna (rt-dna) using a retron reverse transcriptase from exogenous rna |
| EP4499839A1 (en) * | 2022-03-25 | 2025-02-05 | The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone | Methods of assessing engineered retron activity, and uses thereof |
| US20250230429A1 (en) * | 2022-04-07 | 2025-07-17 | The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads | Continuous Multiplexed Phage Genome Engineering Using a Retron Editing Template |
| US20250243516A1 (en) * | 2022-08-24 | 2025-07-31 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David | Dual Cut Retron Editors for Genomic Insertions and Deletions |
| KR20250042094A (ko) | 2023-09-18 | 2025-03-26 | 포항공과대학교 산학협력단 | Dna 결합 단백질 특이적 결합 부위를 포함하는 조작된 레트론 및 이의 용도 |
| WO2025063625A1 (ko) * | 2023-09-18 | 2025-03-27 | 포항공과대학교 산학협력단 | Dna 결합 단백질 특이적 결합 부위를 포함하는 조작된 레트론 및 이의 용도 |
| WO2025128871A2 (en) | 2023-12-13 | 2025-06-19 | Renagade Therapeutics Management Inc. | Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5434070A (en) * | 1989-02-24 | 1995-07-18 | The University Of Medicine And Dentistry Of New Jersey | Reverse transcriptases from Escherichia coli and Myxococcus xanthus |
| WO2003104470A2 (en) * | 2002-06-05 | 2003-12-18 | Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada | Retrons for gene targeting |
| US20050026109A1 (en) * | 2003-07-30 | 2005-02-03 | Buchanan L. Stephen | Multi-tapered dental files |
| US20170204399A1 (en) * | 2014-08-15 | 2017-07-20 | Massachusetts Institute Of Technology | Genomically-encoded memory in live cells |
| KR101922989B1 (ko) * | 2016-05-13 | 2018-11-28 | 연세대학교 산학협력단 | ＣＲＩＳＰＲ／Ｒｅｔｒｏｎ 시스템을 이용한 유전체상의 치환 변이 생성과 추적 방법 |
| ES2985812T3 (es) * | 2016-09-09 | 2024-11-07 | The Board Of Trustees Of The Leland Stanfordjunior Univ | Edición genómica de precisión de alto rendimiento |
| US20180127759A1 (en) * | 2016-10-28 | 2018-05-10 | Massachusetts Institute Of Technology | Dynamic genome engineering |
| WO2018191525A1 (en) * | 2017-04-12 | 2018-10-18 | President And Fellows Of Harvard College | Method of recording multiplexed biological information into a crispr array using a retron |
-
2020
- 2020-09-11 EP EP20863086.3A patent/EP4028512A4/en active Pending
- 2020-09-11 CN CN202510075807.XA patent/CN119859624A/zh active Pending
- 2020-09-11 CN CN202080078530.1A patent/CN114667344A/zh active Pending
- 2020-09-11 JP JP2022516205A patent/JP2022548062A/ja active Pending
- 2020-09-11 AU AU2020346880A patent/AU2020346880B2/en not_active Expired - Fee Related
- 2020-09-11 CA CA3154384A patent/CA3154384A1/en active Pending
- 2020-09-11 US US17/639,043 patent/US20220307007A1/en not_active Abandoned
- 2020-09-11 MX MX2022003091A patent/MX2022003091A/es unknown
- 2020-09-11 WO PCT/US2020/050323 patent/WO2021050822A1/en not_active Ceased
- 2020-09-11 BR BR112022004453A patent/BR112022004453A2/pt unknown
- 2020-09-11 KR KR1020227012030A patent/KR20220081988A/ko not_active Withdrawn
-
2022
- 2022-03-06 IL IL291128A patent/IL291128A/en unknown
- 2022-12-01 US US18/060,790 patent/US20230125704A1/en not_active Abandoned
-
2024
- 2024-05-09 JP JP2024076528A patent/JP2024102277A/ja active Pending
- 2024-11-15 US US18/949,679 patent/US20250188442A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021050822A1 (en) | 2021-03-18 |
| MX2022003091A (es) | 2022-06-08 |
| AU2020346880A1 (en) | 2022-04-21 |
| CA3154384A1 (en) | 2021-03-18 |
| KR20220081988A (ko) | 2022-06-16 |
| JP2022548062A (ja) | 2022-11-16 |
| US20230125704A1 (en) | 2023-04-27 |
| EP4028512A1 (en) | 2022-07-20 |
| IL291128A (en) | 2022-05-01 |
| US20250188442A1 (en) | 2025-06-12 |
| CN119859624A (zh) | 2025-04-22 |
| CN114667344A (zh) | 2022-06-24 |
| EP4028512A4 (en) | 2023-09-20 |
| AU2020346880B2 (en) | 2025-04-03 |
| JP2024102277A (ja) | 2024-07-30 |
| US20220307007A1 (en) | 2022-09-29 |
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