AU2020346880B2 - Modified bacterial retroelement with enhanced DNA production - Google Patents

Modified bacterial retroelement with enhanced DNA production Download PDF

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Publication number
AU2020346880B2
AU2020346880B2 AU2020346880A AU2020346880A AU2020346880B2 AU 2020346880 B2 AU2020346880 B2 AU 2020346880B2 AU 2020346880 A AU2020346880 A AU 2020346880A AU 2020346880 A AU2020346880 A AU 2020346880A AU 2020346880 B2 AU2020346880 B2 AU 2020346880B2
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Prior art keywords
retron
gene
sequence
engineered
cell
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Expired - Fee Related
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AU2020346880A
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AU2020346880A1 (en
Inventor
Seth SHIPMAN
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J David Gladstone Institutes
Harvard University
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J David Gladstone Institutes
Harvard University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2020346880A 2019-09-12 2020-09-11 Modified bacterial retroelement with enhanced DNA production Expired - Fee Related AU2020346880B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962899625P 2019-09-12 2019-09-12
US62/899,625 2019-09-12
PCT/US2020/050323 WO2021050822A1 (en) 2019-09-12 2020-09-11 Modified bacterial retroelement with enhanced dna production

Publications (2)

Publication Number Publication Date
AU2020346880A1 AU2020346880A1 (en) 2022-04-21
AU2020346880B2 true AU2020346880B2 (en) 2025-04-03

Family

ID=74865645

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020346880A Expired - Fee Related AU2020346880B2 (en) 2019-09-12 2020-09-11 Modified bacterial retroelement with enhanced DNA production

Country Status (11)

Country Link
US (3) US20220307007A1 (https=)
EP (1) EP4028512A4 (https=)
JP (2) JP2022548062A (https=)
KR (1) KR20220081988A (https=)
CN (2) CN119859624A (https=)
AU (1) AU2020346880B2 (https=)
BR (1) BR112022004453A2 (https=)
CA (1) CA3154384A1 (https=)
IL (1) IL291128A (https=)
MX (1) MX2022003091A (https=)
WO (1) WO2021050822A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240263173A1 (en) * 2021-08-11 2024-08-08 The Board Of Trustees Of The Leland Stanford Junior University High-throughput precision genome editing in human cells
CN118511225A (zh) * 2021-09-02 2024-08-16 华盛顿大学 多重时间分辨分子信号记录器及相关方法
CA3237482A1 (en) * 2021-11-03 2023-05-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Precise genome editing using retrons
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
EP4499822A1 (en) * 2022-03-25 2025-02-05 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Rt-dna fidelity and retron genome editing
WO2023183627A1 (en) * 2022-03-25 2023-09-28 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Production of reverse transcribed dna (rt-dna) using a retron reverse transcriptase from exogenous rna
EP4499839A1 (en) * 2022-03-25 2025-02-05 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Methods of assessing engineered retron activity, and uses thereof
US20250230429A1 (en) * 2022-04-07 2025-07-17 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads Continuous Multiplexed Phage Genome Engineering Using a Retron Editing Template
US20250243516A1 (en) * 2022-08-24 2025-07-31 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Dual Cut Retron Editors for Genomic Insertions and Deletions
KR20250042094A (ko) 2023-09-18 2025-03-26 포항공과대학교 산학협력단 Dna 결합 단백질 특이적 결합 부위를 포함하는 조작된 레트론 및 이의 용도
WO2025063625A1 (ko) * 2023-09-18 2025-03-27 포항공과대학교 산학협력단 Dna 결합 단백질 특이적 결합 부위를 포함하는 조작된 레트론 및 이의 용도
WO2025128871A2 (en) 2023-12-13 2025-06-19 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090123991A1 (en) * 2002-06-05 2009-05-14 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Retrons for gene targeting
US20180127759A1 (en) * 2016-10-28 2018-05-10 Massachusetts Institute Of Technology Dynamic genome engineering
WO2018191525A1 (en) * 2017-04-12 2018-10-18 President And Fellows Of Harvard College Method of recording multiplexed biological information into a crispr array using a retron

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5434070A (en) * 1989-02-24 1995-07-18 The University Of Medicine And Dentistry Of New Jersey Reverse transcriptases from Escherichia coli and Myxococcus xanthus
US20050026109A1 (en) * 2003-07-30 2005-02-03 Buchanan L. Stephen Multi-tapered dental files
US20170204399A1 (en) * 2014-08-15 2017-07-20 Massachusetts Institute Of Technology Genomically-encoded memory in live cells
KR101922989B1 (ko) * 2016-05-13 2018-11-28 연세대학교 산학협력단 ＣＲＩＳＰＲ／Ｒｅｔｒｏｎ 시스템을 이용한 유전체상의 치환 변이 생성과 추적 방법
ES2985812T3 (es) * 2016-09-09 2024-11-07 The Board Of Trustees Of The Leland Stanfordjunior Univ Edición genómica de precisión de alto rendimiento

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090123991A1 (en) * 2002-06-05 2009-05-14 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Retrons for gene targeting
US20180127759A1 (en) * 2016-10-28 2018-05-10 Massachusetts Institute Of Technology Dynamic genome engineering
WO2018191525A1 (en) * 2017-04-12 2018-10-18 President And Fellows Of Harvard College Method of recording multiplexed biological information into a crispr array using a retron

Also Published As

Publication number Publication date
WO2021050822A1 (en) 2021-03-18
MX2022003091A (es) 2022-06-08
AU2020346880A1 (en) 2022-04-21
CA3154384A1 (en) 2021-03-18
KR20220081988A (ko) 2022-06-16
JP2022548062A (ja) 2022-11-16
US20230125704A1 (en) 2023-04-27
EP4028512A1 (en) 2022-07-20
IL291128A (en) 2022-05-01
US20250188442A1 (en) 2025-06-12
CN119859624A (zh) 2025-04-22
BR112022004453A2 (pt) 2022-06-21
CN114667344A (zh) 2022-06-24
EP4028512A4 (en) 2023-09-20
JP2024102277A (ja) 2024-07-30
US20220307007A1 (en) 2022-09-29

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