BR112021022759A2 - Método para purificar um oligossacarídeo de interesse a partir de um caldo de fermentação - Google Patents
Método para purificar um oligossacarídeo de interesse a partir de um caldo de fermentaçãoInfo
- Publication number
- BR112021022759A2 BR112021022759A2 BR112021022759A BR112021022759A BR112021022759A2 BR 112021022759 A2 BR112021022759 A2 BR 112021022759A2 BR 112021022759 A BR112021022759 A BR 112021022759A BR 112021022759 A BR112021022759 A BR 112021022759A BR 112021022759 A2 BR112021022759 A2 BR 112021022759A2
- Authority
- BR
- Brazil
- Prior art keywords
- oligosaccharide
- interest
- fermentation broth
- providing
- purifying
- Prior art date
Links
- 229920001542 oligosaccharide Polymers 0.000 title abstract 9
- 150000002482 oligosaccharides Chemical class 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 6
- 238000000855 fermentation Methods 0.000 title abstract 5
- 230000004151 fermentation Effects 0.000 title abstract 5
- 238000001914 filtration Methods 0.000 abstract 3
- 239000000706 filtrate Substances 0.000 abstract 2
- 239000012528 membrane Substances 0.000 abstract 2
- 230000000813 microbial effect Effects 0.000 abstract 2
- 238000001728 nano-filtration Methods 0.000 abstract 2
- 239000002028 Biomass Substances 0.000 abstract 1
- 150000001720 carbohydrates Chemical class 0.000 abstract 1
- 235000014633 carbohydrates Nutrition 0.000 abstract 1
- 238000000909 electrodialysis Methods 0.000 abstract 1
- 235000020256 human milk Nutrition 0.000 abstract 1
- 210000004251 human milk Anatomy 0.000 abstract 1
- 230000007935 neutral effect Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000012465 retentate Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/269—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of microbial origin, e.g. xanthan or dextran
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/40—Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
- B01D61/025—Reverse osmosis; Hyperfiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
- B01D61/025—Reverse osmosis; Hyperfiltration
- B01D61/026—Reverse osmosis; Hyperfiltration comprising multiple reverse osmosis steps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
- B01D61/027—Nanofiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
- B01D61/146—Ultrafiltration comprising multiple ultrafiltration steps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/147—Microfiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/42—Electrodialysis; Electro-osmosis ; Electro-ultrafiltration; Membrane capacitive deionization
- B01D61/422—Electrodialysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/58—Multistep processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2311/00—Details relating to membrane separation process operations and control
- B01D2311/04—Specific process operations in the feed stream; Feed pretreatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2311/00—Details relating to membrane separation process operations and control
- B01D2311/06—Specific process operations in the permeate stream
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2311/00—Details relating to membrane separation process operations and control
- B01D2311/26—Further operations combined with membrane separation processes
- B01D2311/2626—Absorption or adsorption
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2311/00—Details relating to membrane separation process operations and control
- B01D2311/26—Further operations combined with membrane separation processes
- B01D2311/2676—Centrifugal separation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2315/00—Details relating to the membrane module operation
- B01D2315/16—Diafiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2325/00—Details relating to properties of membranes
- B01D2325/20—Specific permeability or cut-off range
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Nanotechnology (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Sustainable Development (AREA)
- Medicinal Chemistry (AREA)
- Materials Engineering (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Pediatric Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Dispersion Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
purificação de oligossacarídeos a partir de um caldo de fermentação utilizando filtração. um método para purificar um oligossacarídeo, preferencialmente um oligossacarídeo do leite humano (hmo) neutro ou um hmo sialilado, a partir de um caldo de fermentação, o método compreendendo: - fornecer um caldo de fermentação que contém o oligossacarídeo de interesse, biomassa, células microbianas e carboidratos diferentes do oligossacarídeo de interesse; - remover as células microbianas a partir do caldo de fermentação, fornecendo assim um fluxo de processo; - submeter o fluxo de processo a uma primeira etapa de filtragem usando uma membrana de nanofiltração, fornecendo assim um filtrado que contém o oligossacarídeo de interesse; - submeter o filtrado a uma segunda etapa de filtração usando uma membrana de nanofiltração, fornecendo assim um retentado que contém o oligossacarídeo de interesse; e - remover os sais a partir do fluxo de processo usando eletrodiálise, fornecendo assim uma preparação purificada do oligossacarídeo de interesse.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP19175716.0A EP3741770A1 (en) | 2019-05-21 | 2019-05-21 | Purification of oligosaccharides from a fermentation broth by using filtration |
PCT/EP2020/061888 WO2020233958A1 (en) | 2019-05-21 | 2020-04-29 | Purification of oligosaccharides from a fermentation broth by using filtration |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112021022759A2 true BR112021022759A2 (pt) | 2022-01-11 |
Family
ID=66630128
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112021022759A BR112021022759A2 (pt) | 2019-05-21 | 2020-04-29 | Método para purificar um oligossacarídeo de interesse a partir de um caldo de fermentação |
Country Status (10)
Country | Link |
---|---|
US (1) | US20220251130A1 (pt) |
EP (2) | EP3741770A1 (pt) |
JP (1) | JP2022533240A (pt) |
KR (1) | KR20220016814A (pt) |
CN (2) | CN113811537A (pt) |
AU (1) | AU2020279483A1 (pt) |
BR (1) | BR112021022759A2 (pt) |
MX (1) | MX2021014169A (pt) |
SG (1) | SG11202111535QA (pt) |
WO (1) | WO2020233958A1 (pt) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FI3801037T3 (fi) | 2018-06-01 | 2023-01-13 | Yksinkertainen menetelmä sialylaktooinin puhdistamiseen | |
WO2022263425A1 (en) * | 2021-06-15 | 2022-12-22 | Dsm Ip Assets B.V. | Separation of human milk oligosaccharides from a fermentation broth |
WO2022263406A1 (en) * | 2021-06-15 | 2022-12-22 | Dsm Ip Assets B.V. | Separation of human milk oligosaccharides from a fermentation broth |
BE1029437B1 (nl) * | 2021-06-15 | 2023-07-31 | Dsm Ip Assets Bv | Scheiding van moedermelkoligosachariden uit een fermentatiebouillon |
BE1029435B1 (nl) * | 2021-06-15 | 2023-07-31 | Dsm Ip Assets Bv | Scheiding van moedermelkoligosachariden uit een fermentatiebouillon |
WO2024047096A1 (en) | 2022-08-30 | 2024-03-07 | Inbiose N.V. | Process for purification of an oligosaccharide |
CN118047820A (zh) * | 2024-04-16 | 2024-05-17 | 北京三元食品股份有限公司 | 一种3-岩藻糖基乳糖的制备方法及制备的标准品 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2796082B1 (fr) | 1999-07-07 | 2003-06-27 | Centre Nat Rech Scient | Procede de production d'oligosaccharides |
US7820422B2 (en) | 2005-06-16 | 2010-10-26 | Centre National De La Recherche Scientifique (Cnrs) | Efficient production of oligosaccharides using metabolically engineered microorganisms |
GB0904562D0 (en) | 2009-03-17 | 2009-04-29 | Separation Technologies Invest | Isolation and purification of components of whey |
BRPI1013822A2 (pt) | 2009-04-07 | 2015-08-25 | Glycom As | Método para síntese de um trissacarídeo. |
EP2857410A1 (en) | 2013-10-04 | 2015-04-08 | Jennewein Biotechnologie GmbH | Process for purification of 2´-fucosyllactose using simulated moving bed chromatography |
EP2896628B1 (en) * | 2014-01-20 | 2018-09-19 | Jennewein Biotechnologie GmbH | Process for efficient purification of neutral human milk oligosaccharides (HMOs) from microbial fermentation |
WO2016095924A1 (en) | 2014-12-16 | 2016-06-23 | Glycom A/S | Separation of 2'-fl from a fermentation broth |
NZ750365A (en) | 2016-07-28 | 2022-07-01 | Fonterra Cooperative Group Ltd | Dairy product and process |
EP3450443A1 (en) * | 2017-08-29 | 2019-03-06 | Jennewein Biotechnologie GmbH | Process for purifying sialylated oligosaccharides |
JP2021505171A (ja) * | 2017-12-08 | 2021-02-18 | イェンネワイン バイオテクノロジー ゲーエムベーハーJennewein Biotechnologie GmbH | 噴霧乾燥シアリルラクトース |
-
2019
- 2019-05-21 EP EP19175716.0A patent/EP3741770A1/en not_active Withdrawn
-
2020
- 2020-04-29 BR BR112021022759A patent/BR112021022759A2/pt unknown
- 2020-04-29 CN CN202080035253.6A patent/CN113811537A/zh active Pending
- 2020-04-29 KR KR1020217036037A patent/KR20220016814A/ko unknown
- 2020-04-29 EP EP20720847.1A patent/EP3980435A1/en active Pending
- 2020-04-29 US US17/612,511 patent/US20220251130A1/en active Pending
- 2020-04-29 MX MX2021014169A patent/MX2021014169A/es unknown
- 2020-04-29 SG SG11202111535QA patent/SG11202111535QA/en unknown
- 2020-04-29 WO PCT/EP2020/061888 patent/WO2020233958A1/en unknown
- 2020-04-29 CN CN202410142949.9A patent/CN118084989A/zh active Pending
- 2020-04-29 JP JP2021569214A patent/JP2022533240A/ja active Pending
- 2020-04-29 AU AU2020279483A patent/AU2020279483A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CN113811537A (zh) | 2021-12-17 |
WO2020233958A1 (en) | 2020-11-26 |
CN118084989A (zh) | 2024-05-28 |
EP3741770A1 (en) | 2020-11-25 |
MX2021014169A (es) | 2022-01-04 |
EP3980435A1 (en) | 2022-04-13 |
AU2020279483A1 (en) | 2022-01-06 |
US20220251130A1 (en) | 2022-08-11 |
KR20220016814A (ko) | 2022-02-10 |
SG11202111535QA (en) | 2021-12-30 |
JP2022533240A (ja) | 2022-07-21 |
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