BR112021019640A2 - Bibliotecas de células únicas de alto rendimento e métodos de preparo e uso - Google Patents
Bibliotecas de células únicas de alto rendimento e métodos de preparo e usoInfo
- Publication number
- BR112021019640A2 BR112021019640A2 BR112021019640A BR112021019640A BR112021019640A2 BR 112021019640 A2 BR112021019640 A2 BR 112021019640A2 BR 112021019640 A BR112021019640 A BR 112021019640A BR 112021019640 A BR112021019640 A BR 112021019640A BR 112021019640 A2 BR112021019640 A2 BR 112021019640A2
- Authority
- BR
- Brazil
- Prior art keywords
- methods
- preparation
- single cell
- nucleic acids
- cell libraries
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962950670P | 2019-12-19 | 2019-12-19 | |
| PCT/US2020/066013 WO2021127436A2 (en) | 2019-12-19 | 2020-12-18 | High-throughput single-cell libraries and methods of making and of using |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112021019640A2 true BR112021019640A2 (pt) | 2022-06-21 |
Family
ID=74191887
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021019640A BR112021019640A2 (pt) | 2019-12-19 | 2020-12-18 | Bibliotecas de células únicas de alto rendimento e métodos de preparo e uso |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20220356461A1 (https=) |
| EP (1) | EP3927824A2 (https=) |
| JP (2) | JP2023508792A (https=) |
| KR (1) | KR20220118295A (https=) |
| CN (1) | CN114008199A (https=) |
| AU (1) | AU2020407641A1 (https=) |
| BR (1) | BR112021019640A2 (https=) |
| CA (1) | CA3134746A1 (https=) |
| IL (1) | IL286643A (https=) |
| MX (2) | MX2021011847A (https=) |
| SG (1) | SG11202109486QA (https=) |
| WO (1) | WO2021127436A2 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20240287505A1 (en) * | 2021-06-24 | 2024-08-29 | Illumina, Inc. | Methods and compositions for combinatorial indexing of bead-based nucleic acids |
| CN114121158A (zh) * | 2021-12-01 | 2022-03-01 | 湖南大学 | 一种基于深度网络自适应的scRNA-seq细胞类型识别方法 |
| EP4463567A4 (en) * | 2022-01-12 | 2026-02-18 | Jumpcode Genomics Inc | METHODS AND COMPOSITIONS FOR TRANSCRIPTOME ANALYSIS |
| CN115064220B (zh) * | 2022-06-14 | 2025-05-16 | 浙江大学 | 一种单细胞的跨物种细胞类型鉴定方法 |
| WO2024019988A1 (en) * | 2022-07-18 | 2024-01-25 | Cellsbin, Inc. | Devices for biological analysis |
| CN118460688A (zh) * | 2023-02-09 | 2024-08-09 | 中国人民解放军军事科学院军事医学研究院 | 多聚甲醛固定细胞的单细胞转录组及多重靶向蛋白质同时检测方法的建立 |
| WO2024250155A1 (zh) * | 2023-06-05 | 2024-12-12 | 清华大学 | 一种构建单细胞测序文库的方法 |
| CN117802091B (zh) * | 2023-12-29 | 2026-02-06 | 江苏农牧科技职业学院 | 一种地方鹅t2t基因组组装方法 |
| CN120442620B (zh) * | 2025-07-11 | 2025-10-31 | 良渚实验室 | 用于蛋白检测体系的寡核苷酸阻断剂及降低基于邻位反应的超灵敏蛋白检测体系背景的方法 |
Family Cites Families (82)
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| CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
| CA1341584C (en) | 1988-04-06 | 2008-11-18 | Bruce Wallace | Method of amplifying and detecting nucleic acid sequences |
| AU3539089A (en) | 1988-04-08 | 1989-11-03 | Salk Institute For Biological Studies, The | Ligase-based amplification method |
| ATE144556T1 (de) | 1988-06-24 | 1996-11-15 | Amgen Inc | Verfahren und mittel zum nachweis von nukleinsäuresequenzen |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
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| IL271454B2 (en) * | 2018-05-17 | 2025-04-01 | Illumina Inc | Rapid single-cell genetic sequencing with low Aggregation bias |
| BR112021006234A2 (pt) | 2019-03-01 | 2021-09-28 | Illumina, Inc. | Bibliotecas de células únicas e núcleos únicos de alto rendimento e métodos de preparo e uso |
-
2020
- 2020-12-18 JP JP2021557409A patent/JP2023508792A/ja active Pending
- 2020-12-18 US US17/441,741 patent/US20220356461A1/en active Pending
- 2020-12-18 MX MX2021011847A patent/MX2021011847A/es unknown
- 2020-12-18 WO PCT/US2020/066013 patent/WO2021127436A2/en not_active Ceased
- 2020-12-18 SG SG11202109486QA patent/SG11202109486QA/en unknown
- 2020-12-18 CN CN202080026206.5A patent/CN114008199A/zh active Pending
- 2020-12-18 CA CA3134746A patent/CA3134746A1/en active Pending
- 2020-12-18 KR KR1020217030969A patent/KR20220118295A/ko active Pending
- 2020-12-18 EP EP20842799.7A patent/EP3927824A2/en active Pending
- 2020-12-18 AU AU2020407641A patent/AU2020407641A1/en active Pending
- 2020-12-18 BR BR112021019640A patent/BR112021019640A2/pt unknown
-
2021
- 2021-09-23 IL IL286643A patent/IL286643A/en unknown
- 2021-09-28 MX MX2025014294A patent/MX2025014294A/es unknown
-
2025
- 2025-04-21 JP JP2025069356A patent/JP2025108660A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN114008199A (zh) | 2022-02-01 |
| WO2021127436A2 (en) | 2021-06-24 |
| MX2025014294A (es) | 2026-01-07 |
| WO2021127436A3 (en) | 2021-07-29 |
| US20220356461A1 (en) | 2022-11-10 |
| AU2020407641A1 (en) | 2021-09-23 |
| SG11202109486QA (en) | 2021-09-29 |
| EP3927824A2 (en) | 2021-12-29 |
| JP2025108660A (ja) | 2025-07-23 |
| KR20220118295A (ko) | 2022-08-25 |
| JP2023508792A (ja) | 2023-03-06 |
| IL286643A (en) | 2021-12-01 |
| MX2021011847A (es) | 2021-11-17 |
| CA3134746A1 (en) | 2021-06-24 |
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