BR112020024153A2 - Cepa mutante e métodos de produção de proteína, celulase e açúcar - Google Patents

Cepa mutante e métodos de produção de proteína, celulase e açúcar Download PDF

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Publication number
BR112020024153A2
BR112020024153A2 BR112020024153-9A BR112020024153A BR112020024153A2 BR 112020024153 A2 BR112020024153 A2 BR 112020024153A2 BR 112020024153 A BR112020024153 A BR 112020024153A BR 112020024153 A2 BR112020024153 A2 BR 112020024153A2
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BR
Brazil
Prior art keywords
strain
mutant
seq
amino acid
acid sequence
Prior art date
Application number
BR112020024153-9A
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English (en)
Portuguese (pt)
Inventor
Yusuke KAGAWA
Shingo Hiramatsu
Katsushige Yamada
Original Assignee
Toray Industries, Inc.
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Publication date
Application filed by Toray Industries, Inc. filed Critical Toray Industries, Inc.
Publication of BR112020024153A2 publication Critical patent/BR112020024153A2/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/885Trichoderma

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BR112020024153-9A 2018-05-31 2019-05-30 Cepa mutante e métodos de produção de proteína, celulase e açúcar BR112020024153A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018105256 2018-05-31
JP2018-105256 2018-05-31
PCT/JP2019/021449 WO2019230860A1 (ja) 2018-05-31 2019-05-30 トリコデルマ属糸状菌変異株およびタンパク質の製造方法

Publications (1)

Publication Number Publication Date
BR112020024153A2 true BR112020024153A2 (pt) 2021-03-30

Family

ID=68698927

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112020024153-9A BR112020024153A2 (pt) 2018-05-31 2019-05-30 Cepa mutante e métodos de produção de proteína, celulase e açúcar

Country Status (8)

Country Link
US (1) US11608493B2 (https=)
EP (1) EP3805365A4 (https=)
JP (1) JP7400468B2 (https=)
CN (1) CN112243458A (https=)
AU (1) AU2019277550B2 (https=)
BR (1) BR112020024153A2 (https=)
CA (1) CA3102065A1 (https=)
WO (1) WO2019230860A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3778864A4 (en) * 2018-03-26 2021-12-15 Toray Industries, Inc. TRICHODERMA REESEI MUTANT AND PROTEIN PRODUCTION PROCESS
US20250179459A1 (en) 2022-03-08 2025-06-05 Toray Industries, Inc. Filter aid, method of filtration treatment, and method of manufacturing cellulase

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX350399B (es) * 2009-12-18 2017-09-06 Novozymes Inc Metodos para producir polipeptidos en mutantes de trichoderma deficientes en proteasa.
CN102311951B (zh) * 2011-07-13 2013-03-20 深圳大学 里氏木霉组成型表达盒、表达载体、重组菌株及其应用
JP2013220069A (ja) * 2012-04-17 2013-10-28 Nagaoka Univ Of Technology 植物性多糖の分解活性が増強された糸状菌及び該糸状菌の使用
JP2014150745A (ja) * 2013-02-06 2014-08-25 Nagaoka Univ Of Technology トリコデルマ属に属する微生物の変異株および該変異株の使用
WO2014202616A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
JP2016106538A (ja) * 2014-12-03 2016-06-20 東レ株式会社 トリコデルマ・リーセイ変異株およびそれを用いたセロビオハイドラーゼの製造方法
BR112018069721A2 (pt) 2016-03-31 2019-02-05 Toray Industries fungo, métodos para produzir uma composição de celulase e para produzir glicose e xilo-oligossacarídeos

Also Published As

Publication number Publication date
JPWO2019230860A1 (ja) 2021-04-22
US11608493B2 (en) 2023-03-21
EP3805365A1 (en) 2021-04-14
AU2019277550A1 (en) 2020-12-24
CA3102065A1 (en) 2019-12-05
JP7400468B2 (ja) 2023-12-19
CN112243458A (zh) 2021-01-19
WO2019230860A1 (ja) 2019-12-05
US20210214403A1 (en) 2021-07-15
AU2019277550B2 (en) 2025-03-06
EP3805365A4 (en) 2022-03-16

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