BR112019021009A2 - peptídeo ligase e uso do mesmo - Google Patents

peptídeo ligase e uso do mesmo Download PDF

Info

Publication number
BR112019021009A2
BR112019021009A2 BR112019021009A BR112019021009A BR112019021009A2 BR 112019021009 A2 BR112019021009 A2 BR 112019021009A2 BR 112019021009 A BR112019021009 A BR 112019021009A BR 112019021009 A BR112019021009 A BR 112019021009A BR 112019021009 A2 BR112019021009 A2 BR 112019021009A2
Authority
BR
Brazil
Prior art keywords
peptide
seq
polypeptide
tag
amino acid
Prior art date
Application number
BR112019021009A
Other languages
English (en)
Portuguese (pt)
Inventor
M Buldun Can
Howarth Mark
Original Assignee
Univ Oxford Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Oxford Innovation Ltd filed Critical Univ Oxford Innovation Ltd
Publication of BR112019021009A2 publication Critical patent/BR112019021009A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3156Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K17/00Carrier-bound or immobilised peptides; Preparation thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185Escherichia
    • C12R2001/19Escherichia coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Pulmonology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR112019021009A 2017-04-10 2018-04-10 peptídeo ligase e uso do mesmo BR112019021009A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1705750.6A GB201705750D0 (en) 2017-04-10 2017-04-10 Peptide ligase and use therof
PCT/GB2018/050943 WO2018189517A1 (en) 2017-04-10 2018-04-10 Peptide ligase and use thereof

Publications (1)

Publication Number Publication Date
BR112019021009A2 true BR112019021009A2 (pt) 2020-05-05

Family

ID=58744859

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019021009A BR112019021009A2 (pt) 2017-04-10 2018-04-10 peptídeo ligase e uso do mesmo

Country Status (12)

Country Link
US (1) US10889622B2 (https=)
EP (1) EP3610005B1 (https=)
JP (1) JP7075678B2 (https=)
KR (1) KR102669378B1 (https=)
CN (2) CN110582566B (https=)
AU (1) AU2018251237B2 (https=)
BR (1) BR112019021009A2 (https=)
CA (1) CA3059132A1 (https=)
DK (1) DK3610005T3 (https=)
ES (1) ES2900612T3 (https=)
GB (1) GB201705750D0 (https=)
WO (1) WO2018189517A1 (https=)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3774926A1 (en) 2018-04-05 2021-02-17 Bio-Rad ABD Serotec GmbH Display systems for proteins of interest
CN112638411B (zh) 2018-05-04 2025-03-04 斯拜生物技术有限公司 疫苗组合物
GB201819850D0 (en) 2018-12-05 2019-01-23 Univ Oxford Innovation Ltd Polypeptide and its use in affinity purification
GB201903479D0 (en) 2019-03-14 2019-05-01 Univ Oxford Innovation Ltd Polypeptide with enchanced rate of spontaneous isopeptide bond formation with it's peptide tag partner and uses thereof
EP3942079A1 (en) 2019-03-18 2022-01-26 Bio-Rad ABD Serotec GmbH Protection of spytag-containing periplasmic fusion proteins from protease tsp and ompt degradation
GB201915905D0 (en) 2019-11-01 2019-12-18 Spybiotech Ltd Viruses with modified capsid proteins
US12298314B2 (en) 2020-10-09 2025-05-13 10X Genomics, Inc. Methods and compositions for analyzing target binding of molecules
WO2022076914A1 (en) 2020-10-09 2022-04-14 10X Genomics, Inc. Methods and compositions for profiling immune repertoire
GB202019817D0 (en) 2020-12-15 2021-01-27 Univ Oxford Innovation Ltd Ligand-binding polypeptides and uses thereof
CN112877347B (zh) * 2021-01-28 2023-02-17 华南理工大学 一种多酶复合体及其构建方法和应用
GB202104104D0 (en) 2021-03-24 2021-05-05 Liliumx Ltd Platform and method
GB202104999D0 (en) * 2021-04-08 2021-05-26 Univ Oxford Innovation Ltd Polypeptides that interact with peptide tags at loops or termini and uses thereof
US20240240203A1 (en) 2021-05-04 2024-07-18 SpyBiotech Limited Adenoviral vectors and vaccines thereof
GB202106361D0 (en) 2021-05-04 2021-06-16 Spybiotech Ltd Viral vectored vaccines
GB202117283D0 (en) 2021-11-30 2022-01-12 Univ Oxford Innovation Ltd Switchable polypeptide and its use for gentle affinity purification
US12043862B2 (en) 2021-12-28 2024-07-23 Encodia, Inc. High-throughput serotyping and antibody profiling assays
JP2025534309A (ja) 2022-09-28 2025-10-15 ヴァリンク セラピューティクス リミテッド 多価タンパク質およびスクリーニング法
GB202306808D0 (en) 2023-05-09 2023-06-21 Univ Oxford Innovation Ltd Method for detecting interactions
GB202313175D0 (en) 2023-08-30 2023-10-11 Univ Oxford Innovation Ltd A bivalent polypeptide scaffold and uses thereof
TW202528546A (zh) 2023-09-07 2025-07-16 美商再生元醫藥公司 經共價表面修飾之腺相關病毒的生產及純化
WO2025083285A1 (en) 2023-10-20 2025-04-24 Norwegian University Of Science And Technology (Ntnu) Methods and products for use in genome-wide chromatin profiling
TW202540433A (zh) 2023-11-21 2025-10-16 美商再生元醫藥公司 藉由活體外接合產生共價表面修飾之腺相關病毒及共價表面修飾的腺相關病毒之純化
GB202317925D0 (en) 2023-11-23 2024-01-10 Spybiotech Ltd Vaccines
WO2025250838A1 (en) * 2024-05-31 2025-12-04 University Of Washington Boolean logic-gated protein presentation via autonomously compiled molecular topology
WO2026050618A1 (en) 2024-08-29 2026-03-05 Regeneron Pharmaceuticals, Inc. Polynucleotide constructs and cell lines for recombinant aav production

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE414775T1 (de) * 1997-05-16 2008-12-15 Novozymes Inc Polypeptide mit prolyldipeptidylaminopeptidase- aktivität und dafür kodierende nukleinsäuren
GB0714963D0 (en) 2007-08-01 2007-09-12 Novartis Ag Compositions comprising antigens
CN103897045A (zh) 2009-01-12 2014-07-02 诺华股份有限公司 抗革兰氏阳性细菌疫苗中的Cna_B结构域
GB201002362D0 (en) * 2010-02-11 2010-03-31 Isis Innovation Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds
WO2013124473A1 (en) * 2012-02-24 2013-08-29 Novartis Ag Pilus proteins and compositions
WO2015185592A2 (en) * 2014-06-03 2015-12-10 Dsm Ip Assets B.V. Proline-specific endoprotease and use thereof
DK3152304T3 (da) * 2014-06-03 2019-10-14 Dsm Ip Assets Bv Prolinspecifik endoprotease og anvendelse deraf
WO2016112921A1 (en) 2015-01-15 2016-07-21 University Of Copenhagen Virus-like particle with efficient epitope display
GB201509782D0 (en) * 2015-06-05 2015-07-22 Isis Innovation Methods and products for fusion protein synthesis

Also Published As

Publication number Publication date
CN118389457A (zh) 2024-07-26
KR20190137820A (ko) 2019-12-11
CN110582566A (zh) 2019-12-17
JP7075678B2 (ja) 2022-05-26
WO2018189517A1 (en) 2018-10-18
AU2018251237B2 (en) 2024-01-18
US10889622B2 (en) 2021-01-12
EP3610005A1 (en) 2020-02-19
AU2018251237A1 (en) 2019-10-24
CA3059132A1 (en) 2018-10-18
CN110582566B (zh) 2024-05-10
EP3610005B1 (en) 2021-09-29
JP2020516314A (ja) 2020-06-11
US20200115422A1 (en) 2020-04-16
DK3610005T3 (da) 2021-12-13
GB201705750D0 (en) 2017-05-24
ES2900612T3 (es) 2022-03-17
KR102669378B1 (ko) 2024-05-24

Similar Documents

Publication Publication Date Title
BR112019021009A2 (pt) peptídeo ligase e uso do mesmo
ES2887004T3 (es) Proteínas y etiquetas peptídicas con velocidad aumentada de formación espontánea de enlace isopeptídico y su uso
AU2016272543B2 (en) Methods and products for fusion protein synthesis
Weiser et al. Investigation of N-terminal phospho-regulation of uracil DNA glycosylase using protein semisynthesis
CA2987821C (en) Methods and products for fusion protein synthesis
WO2024151615A1 (en) Mrna display libraries and methods of use
CN120118195A (zh) 噬菌体裂解酶类似物及其应用
JPS59173084A (ja) ポリオウイルスの免疫原性部位よりなるペプチド類及びこれらのペプチド類を暗合化するヌクレオチド配列を含有するdna類
HK40027506A (en) Proteins and peptide tags with enhanced rate of spontaneous isopeptide bond formation and uses thereof
HK40027506B (en) Proteins and peptide tags with enhanced rate of spontaneous isopeptide bond formation and uses thereof
BR112017026042B1 (pt) Proteína de fusão e seu método de produção, ligante de peptídeo, par de ligantes de peptídeo e uso dos mesmos, polipeptídeo recombinante ou sintético, molécula de ácido nucleico, vetor, célula hospedeira recombinante e kit

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]