CA3059132A1 - Peptide ligase and use thereof - Google Patents
Peptide ligase and use thereof Download PDFInfo
- Publication number
- CA3059132A1 CA3059132A1 CA3059132A CA3059132A CA3059132A1 CA 3059132 A1 CA3059132 A1 CA 3059132A1 CA 3059132 A CA3059132 A CA 3059132A CA 3059132 A CA3059132 A CA 3059132A CA 3059132 A1 CA3059132 A1 CA 3059132A1
- Authority
- CA
- Canada
- Prior art keywords
- peptide
- seq
- polypeptide
- amino acid
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Pulmonology (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1705750.6 | 2017-04-10 | ||
| GBGB1705750.6A GB201705750D0 (en) | 2017-04-10 | 2017-04-10 | Peptide ligase and use therof |
| PCT/GB2018/050943 WO2018189517A1 (en) | 2017-04-10 | 2018-04-10 | Peptide ligase and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3059132A1 true CA3059132A1 (en) | 2018-10-18 |
Family
ID=58744859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3059132A Pending CA3059132A1 (en) | 2017-04-10 | 2018-04-10 | Peptide ligase and use thereof |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10889622B2 (https=) |
| EP (1) | EP3610005B1 (https=) |
| JP (1) | JP7075678B2 (https=) |
| KR (1) | KR102669378B1 (https=) |
| CN (2) | CN110582566B (https=) |
| AU (1) | AU2018251237B2 (https=) |
| BR (1) | BR112019021009A2 (https=) |
| CA (1) | CA3059132A1 (https=) |
| DK (1) | DK3610005T3 (https=) |
| ES (1) | ES2900612T3 (https=) |
| GB (1) | GB201705750D0 (https=) |
| WO (1) | WO2018189517A1 (https=) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3774926A1 (en) | 2018-04-05 | 2021-02-17 | Bio-Rad ABD Serotec GmbH | Display systems for proteins of interest |
| CN112638411B (zh) | 2018-05-04 | 2025-03-04 | 斯拜生物技术有限公司 | 疫苗组合物 |
| GB201819850D0 (en) | 2018-12-05 | 2019-01-23 | Univ Oxford Innovation Ltd | Polypeptide and its use in affinity purification |
| GB201903479D0 (en) | 2019-03-14 | 2019-05-01 | Univ Oxford Innovation Ltd | Polypeptide with enchanced rate of spontaneous isopeptide bond formation with it's peptide tag partner and uses thereof |
| EP3942079A1 (en) | 2019-03-18 | 2022-01-26 | Bio-Rad ABD Serotec GmbH | Protection of spytag-containing periplasmic fusion proteins from protease tsp and ompt degradation |
| GB201915905D0 (en) | 2019-11-01 | 2019-12-18 | Spybiotech Ltd | Viruses with modified capsid proteins |
| US12298314B2 (en) | 2020-10-09 | 2025-05-13 | 10X Genomics, Inc. | Methods and compositions for analyzing target binding of molecules |
| WO2022076914A1 (en) | 2020-10-09 | 2022-04-14 | 10X Genomics, Inc. | Methods and compositions for profiling immune repertoire |
| GB202019817D0 (en) | 2020-12-15 | 2021-01-27 | Univ Oxford Innovation Ltd | Ligand-binding polypeptides and uses thereof |
| CN112877347B (zh) * | 2021-01-28 | 2023-02-17 | 华南理工大学 | 一种多酶复合体及其构建方法和应用 |
| GB202104104D0 (en) | 2021-03-24 | 2021-05-05 | Liliumx Ltd | Platform and method |
| GB202104999D0 (en) * | 2021-04-08 | 2021-05-26 | Univ Oxford Innovation Ltd | Polypeptides that interact with peptide tags at loops or termini and uses thereof |
| US20240240203A1 (en) | 2021-05-04 | 2024-07-18 | SpyBiotech Limited | Adenoviral vectors and vaccines thereof |
| GB202106361D0 (en) | 2021-05-04 | 2021-06-16 | Spybiotech Ltd | Viral vectored vaccines |
| GB202117283D0 (en) | 2021-11-30 | 2022-01-12 | Univ Oxford Innovation Ltd | Switchable polypeptide and its use for gentle affinity purification |
| US12043862B2 (en) | 2021-12-28 | 2024-07-23 | Encodia, Inc. | High-throughput serotyping and antibody profiling assays |
| JP2025534309A (ja) | 2022-09-28 | 2025-10-15 | ヴァリンク セラピューティクス リミテッド | 多価タンパク質およびスクリーニング法 |
| GB202306808D0 (en) | 2023-05-09 | 2023-06-21 | Univ Oxford Innovation Ltd | Method for detecting interactions |
| GB202313175D0 (en) | 2023-08-30 | 2023-10-11 | Univ Oxford Innovation Ltd | A bivalent polypeptide scaffold and uses thereof |
| TW202528546A (zh) | 2023-09-07 | 2025-07-16 | 美商再生元醫藥公司 | 經共價表面修飾之腺相關病毒的生產及純化 |
| WO2025083285A1 (en) | 2023-10-20 | 2025-04-24 | Norwegian University Of Science And Technology (Ntnu) | Methods and products for use in genome-wide chromatin profiling |
| TW202540433A (zh) | 2023-11-21 | 2025-10-16 | 美商再生元醫藥公司 | 藉由活體外接合產生共價表面修飾之腺相關病毒及共價表面修飾的腺相關病毒之純化 |
| GB202317925D0 (en) | 2023-11-23 | 2024-01-10 | Spybiotech Ltd | Vaccines |
| WO2025250838A1 (en) * | 2024-05-31 | 2025-12-04 | University Of Washington | Boolean logic-gated protein presentation via autonomously compiled molecular topology |
| WO2026050618A1 (en) | 2024-08-29 | 2026-03-05 | Regeneron Pharmaceuticals, Inc. | Polynucleotide constructs and cell lines for recombinant aav production |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE414775T1 (de) * | 1997-05-16 | 2008-12-15 | Novozymes Inc | Polypeptide mit prolyldipeptidylaminopeptidase- aktivität und dafür kodierende nukleinsäuren |
| GB0714963D0 (en) | 2007-08-01 | 2007-09-12 | Novartis Ag | Compositions comprising antigens |
| CN103897045A (zh) | 2009-01-12 | 2014-07-02 | 诺华股份有限公司 | 抗革兰氏阳性细菌疫苗中的Cna_B结构域 |
| GB201002362D0 (en) * | 2010-02-11 | 2010-03-31 | Isis Innovation | Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds |
| WO2013124473A1 (en) * | 2012-02-24 | 2013-08-29 | Novartis Ag | Pilus proteins and compositions |
| WO2015185592A2 (en) * | 2014-06-03 | 2015-12-10 | Dsm Ip Assets B.V. | Proline-specific endoprotease and use thereof |
| DK3152304T3 (da) * | 2014-06-03 | 2019-10-14 | Dsm Ip Assets Bv | Prolinspecifik endoprotease og anvendelse deraf |
| WO2016112921A1 (en) | 2015-01-15 | 2016-07-21 | University Of Copenhagen | Virus-like particle with efficient epitope display |
| GB201509782D0 (en) * | 2015-06-05 | 2015-07-22 | Isis Innovation | Methods and products for fusion protein synthesis |
-
2017
- 2017-04-10 GB GBGB1705750.6A patent/GB201705750D0/en not_active Ceased
-
2018
- 2018-04-10 AU AU2018251237A patent/AU2018251237B2/en active Active
- 2018-04-10 KR KR1020197030655A patent/KR102669378B1/ko active Active
- 2018-04-10 DK DK18719246.3T patent/DK3610005T3/da active
- 2018-04-10 CN CN201880024247.3A patent/CN110582566B/zh active Active
- 2018-04-10 CN CN202410483896.7A patent/CN118389457A/zh active Pending
- 2018-04-10 WO PCT/GB2018/050943 patent/WO2018189517A1/en not_active Ceased
- 2018-04-10 ES ES18719246T patent/ES2900612T3/es active Active
- 2018-04-10 BR BR112019021009A patent/BR112019021009A2/pt unknown
- 2018-04-10 EP EP18719246.3A patent/EP3610005B1/en active Active
- 2018-04-10 US US16/603,850 patent/US10889622B2/en active Active
- 2018-04-10 JP JP2020504454A patent/JP7075678B2/ja active Active
- 2018-04-10 CA CA3059132A patent/CA3059132A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN118389457A (zh) | 2024-07-26 |
| BR112019021009A2 (pt) | 2020-05-05 |
| KR20190137820A (ko) | 2019-12-11 |
| CN110582566A (zh) | 2019-12-17 |
| JP7075678B2 (ja) | 2022-05-26 |
| WO2018189517A1 (en) | 2018-10-18 |
| AU2018251237B2 (en) | 2024-01-18 |
| US10889622B2 (en) | 2021-01-12 |
| EP3610005A1 (en) | 2020-02-19 |
| AU2018251237A1 (en) | 2019-10-24 |
| CN110582566B (zh) | 2024-05-10 |
| EP3610005B1 (en) | 2021-09-29 |
| JP2020516314A (ja) | 2020-06-11 |
| US20200115422A1 (en) | 2020-04-16 |
| DK3610005T3 (da) | 2021-12-13 |
| GB201705750D0 (en) | 2017-05-24 |
| ES2900612T3 (es) | 2022-03-17 |
| KR102669378B1 (ko) | 2024-05-24 |
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| AU2016272543B2 (en) | Methods and products for fusion protein synthesis | |
| JP4088584B2 (ja) | 融合タンパク質から目的タンパク質を分離する方法。 | |
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| HK40027506B (en) | Proteins and peptide tags with enhanced rate of spontaneous isopeptide bond formation and uses thereof | |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220929 |
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