BR112019007257A2 - micro-organismos e métodos para a coprodução de etileno glicol e isobuteno - Google Patents

micro-organismos e métodos para a coprodução de etileno glicol e isobuteno

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Publication number
BR112019007257A2
BR112019007257A2 BR112019007257A BR112019007257A BR112019007257A2 BR 112019007257 A2 BR112019007257 A2 BR 112019007257A2 BR 112019007257 A BR112019007257 A BR 112019007257A BR 112019007257 A BR112019007257 A BR 112019007257A BR 112019007257 A2 BR112019007257 A2 BR 112019007257A2
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BR
Brazil
Prior art keywords
isobutene
microorganisms
production
meg
methods
Prior art date
Application number
BR112019007257A
Other languages
English (en)
Inventor
Johannes Koch Daniel
Galzerani Felipe
Schreiner Lopes Mateus
Original Assignee
Braskem Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Braskem Sa filed Critical Braskem Sa
Publication of BR112019007257A2 publication Critical patent/BR112019007257A2/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017—Xylulokinase (2.7.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01047—D-Ribulokinase (2.7.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/02—Aldehyde-lyases (4.1.2)
    • C12Y401/02013—Fructose-bisphosphate aldolase (4.1.2.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a presente invenção refere-se a micro-organismos recombinantes úteis na biossíntese de monoetileno glicol (meg) e isobuteno. o pedido refere-se ainda a micro-organismos recombinantes coexpressando uma via de ramificação c2 e uma via de ramificação c3 para a produção de meg e isobuteno. também são providos métodos de produção de meg e isobuteno utilizando os micro-organismos recombinantes, assim como composições compreendendo os micro-organismos re-combinantes e/ou opcionalmente os produtos meg e isobuteno.
BR112019007257A 2016-10-11 2017-10-11 micro-organismos e métodos para a coprodução de etileno glicol e isobuteno BR112019007257A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662406684P 2016-10-11 2016-10-11
PCT/US2017/056174 WO2018071563A1 (en) 2016-10-11 2017-10-11 Microorganisms and methods for the co-production of ethylene glycol and isobutene

Publications (1)

Publication Number Publication Date
BR112019007257A2 true BR112019007257A2 (pt) 2019-07-02

Family

ID=61905977

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019007257A BR112019007257A2 (pt) 2016-10-11 2017-10-11 micro-organismos e métodos para a coprodução de etileno glicol e isobuteno

Country Status (4)

Country Link
US (1) US20200048662A1 (pt)
EP (1) EP3525930A4 (pt)
BR (1) BR112019007257A2 (pt)
WO (1) WO2018071563A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111434773B (zh) * 2019-01-15 2021-06-18 天津大学 一种高产檀香油的重组酵母菌及其构建方法与应用
WO2020168407A1 (en) * 2019-02-20 2020-08-27 Braskem S.A. Microorganisms and methods for the production of oxygenated compounds from hexoses

Family Cites Families (15)

* Cited by examiner, † Cited by third party
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US7314974B2 (en) * 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
EP1775590A1 (en) * 2005-10-11 2007-04-18 Laboratorios S.A.L.V.A.T., S.A. Non-invasive in vitro method to detect transitional cell carcinoma of the bladder
US20130195886A1 (en) * 2011-11-27 2013-08-01 The Regents Of The University Of Colorado, A Body Corporate Methods for Fructanase and Fructokinase Inhibition
KR101351879B1 (ko) * 2012-02-06 2014-01-22 명지대학교 산학협력단 에탄―１，２―디올 생산 미생물 및 이를 이용한 에탄―１，２―디올 생산 방법
US20130316416A1 (en) * 2012-02-23 2013-11-28 Massachusetts Institute Of Technology Engineering microbes and metabolic pathways for the production of ethylene glycol
US9752170B2 (en) * 2012-04-17 2017-09-05 Petiva Private Limited Method of production of monosaccharides
WO2013163230A2 (en) * 2012-04-24 2013-10-31 Midori Renewables, Inc. Bio-based polymers and methods of producing thereof
GB2583417B (en) * 2012-09-27 2021-03-31 Tate & Lyle Ingredients Americas Llc A protein
BR112015010557A2 (pt) * 2012-11-13 2018-05-22 Bayer Materialscience Ag método para a produção de fenol a partir de fontes renováveis por meio de fermentação.
US9909129B2 (en) * 2012-12-18 2018-03-06 Regents Of The University Of Minnesota Biosynthetic pathways and methods
WO2015035244A1 (en) * 2013-09-05 2015-03-12 Braskem S/A Modified microorganism and methods of using same for producing butadiene and 1-propanol and/or 1,2-propanediol
BR112016008830B1 (pt) * 2013-10-23 2023-02-23 Ajinomoto Co., Inc Método para produzir uma substância alvo
EP3090043B1 (en) * 2013-12-31 2022-02-02 Global Bioenergies 3-hydroxyisovalerate (hiv) synthase variants
CN107075536B (zh) * 2014-09-17 2021-07-02 环球生物能源公司 生产3-羟基-3-甲基丁酸的方法
FR3028529B1 (fr) * 2014-11-19 2016-12-30 Inst Nat De La Rech Agronomique Inra Procede de production d'au moins un metabolite d'interet par transformation d'un pentose dans un microorganisme

Also Published As

Publication number Publication date
EP3525930A1 (en) 2019-08-21
WO2018071563A8 (en) 2018-05-17
WO2018071563A1 (en) 2018-04-19
US20200048662A1 (en) 2020-02-13
EP3525930A4 (en) 2020-07-08

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]