BR112018007591A2 - dispositivo microfluídico de células simples - Google Patents

dispositivo microfluídico de células simples

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Publication number
BR112018007591A2
BR112018007591A2 BR112018007591A BR112018007591A BR112018007591A2 BR 112018007591 A2 BR112018007591 A2 BR 112018007591A2 BR 112018007591 A BR112018007591 A BR 112018007591A BR 112018007591 A BR112018007591 A BR 112018007591A BR 112018007591 A2 BR112018007591 A2 BR 112018007591A2
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BR
Brazil
Prior art keywords
plant cell
microfluidic device
mrna
probe
plant
Prior art date
Application number
BR112018007591A
Other languages
English (en)
Inventor
P Lee Abraham
Lee Do-Hyun
Kumar Wickramasinghe H
Li Xuan
Tao Yinglei
Yun Yue
Original Assignee
Pioneer Hi Bred Int
Univ California
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Application filed by Pioneer Hi Bred Int, Univ California filed Critical Pioneer Hi Bred Int
Publication of BR112018007591A2 publication Critical patent/BR112018007591A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C40—COMBINATORIAL TECHNOLOGY
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    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
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  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

são revelados métodos para obter de maneira não destrutiva o genótipo de uma célula vegetal em uma amostra vegetal. a célula vegetal é isolada de uma amostra de células vegetais usando um dispositivo microfluídico integrado. o dispositivo microfluídico integrado inclui uma armadilha de célula individual localizada a jusante de um canal microfluídico, e o dispositivo microfluídico é configurado para capturar a célula vegetal na armadilha de célula individual. o mrna é extraído da célula vegetal ao colocá-la em contato com uma sonda de microscópio de força atômica (mfa) e atrair o mrna de locais de interesse para a extremidade de sonda usando uma força dieletroforética (dep) sob o campo de corrente alternada (ca) aplicado à sonda. o genótipo da célula vegetal é determinado com base no cdna obtido do mrna extraído. como alternativa, o mrna é analisado para determinar padrões de expressão genética da célula vegetal.
BR112018007591A 2015-10-14 2016-10-12 dispositivo microfluídico de células simples BR112018007591A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562241600P 2015-10-14 2015-10-14
US201662384628P 2016-09-07 2016-09-07
US15/288,969 US9862941B2 (en) 2015-10-14 2016-10-07 Single cell microfluidic device
PCT/US2016/056683 WO2017066351A1 (en) 2015-10-14 2016-10-12 Single cell microfluidic device

Publications (1)

Publication Number Publication Date
BR112018007591A2 true BR112018007591A2 (pt) 2018-10-23

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BR112018007591A BR112018007591A2 (pt) 2015-10-14 2016-10-12 dispositivo microfluídico de células simples

Country Status (9)

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US (2) US9862941B2 (pt)
EP (1) EP3362179B1 (pt)
AU (1) AU2016339949B2 (pt)
BR (1) BR112018007591A2 (pt)
CA (1) CA3001570C (pt)
CL (1) CL2018000961A1 (pt)
MX (1) MX2018004519A (pt)
SG (1) SG11201802954SA (pt)
WO (1) WO2017066351A1 (pt)

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US9862941B2 (en) 2018-01-09
AU2016339949A1 (en) 2018-05-10
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SG11201802954SA (en) 2018-05-30
CA3001570A1 (en) 2017-04-20
EP3362179A4 (en) 2019-03-20
AU2016339949B2 (en) 2021-05-20
EP3362179B1 (en) 2021-03-24
CA3001570C (en) 2022-09-20
WO2017066351A8 (en) 2018-05-03
US20170107507A1 (en) 2017-04-20
US10526595B2 (en) 2020-01-07
EP3362179A1 (en) 2018-08-22
WO2017066351A1 (en) 2017-04-20
CL2018000961A1 (es) 2018-11-16

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