MX2018004519A - Dispositivo microfluidico de celulas individuales. - Google Patents

Dispositivo microfluidico de celulas individuales.

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Publication number
MX2018004519A
MX2018004519A MX2018004519A MX2018004519A MX2018004519A MX 2018004519 A MX2018004519 A MX 2018004519A MX 2018004519 A MX2018004519 A MX 2018004519A MX 2018004519 A MX2018004519 A MX 2018004519A MX 2018004519 A MX2018004519 A MX 2018004519A
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MX
Mexico
Prior art keywords
plant cell
microfluidic device
mrna
trap
probe
Prior art date
Application number
MX2018004519A
Other languages
English (en)
Inventor
Wickramasinghe H Kumar
Li Xuan
Tao Yinglei
Hyun Lee Do-
P Lee Abraham
Yun Yue
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Univ California
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Publication date
Application filed by Univ California filed Critical Univ California
Publication of MX2018004519A publication Critical patent/MX2018004519A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C40—COMBINATORIAL TECHNOLOGY
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    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
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    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
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    • B01L2300/00—Additional constructional details
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2400/08—Regulating or influencing the flow resistance
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    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Se describen métodos para obtener no destructivamente el genotipo de una célula vegetal de una muestra vegetal. La célula vegetal se aísla de una muestra de células vegetales que utiliza un dispositivo microfluídico integrado. El dispositivo microfluídico integrado incluye una trampa celular individual situada corriente abajo de un canal microfluídico y, el dispositivo microfluídico se configura para atrapar la célula vegetal en la trampa celular individual. El ARNm se extrae de la célula vegetal al poner en contacto la célula vegetal con una sonda de microscopio de fuerza atómica (AFM) y al extraer ARNm de los sitios de interés al extremo de la sonda utilizando una fuerza DEP de dielectroforesis bajo el campo de corriente alterna (AC) aplicado a la sonda. El genotipo de la célula vegetal se determina de ADNc obtenido del ARNm extraído. Alternativamente, el ARNm se analiza para determinar los patrones de expresión génica de la célula vegetal.
MX2018004519A 2015-10-14 2016-10-12 Dispositivo microfluidico de celulas individuales. MX2018004519A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562241600P 2015-10-14 2015-10-14
US201662384628P 2016-09-07 2016-09-07
US15/288,969 US9862941B2 (en) 2015-10-14 2016-10-07 Single cell microfluidic device
PCT/US2016/056683 WO2017066351A1 (en) 2015-10-14 2016-10-12 Single cell microfluidic device

Publications (1)

Publication Number Publication Date
MX2018004519A true MX2018004519A (es) 2018-08-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018004519A MX2018004519A (es) 2015-10-14 2016-10-12 Dispositivo microfluidico de celulas individuales.

Country Status (9)

Country Link
US (2) US9862941B2 (es)
EP (1) EP3362179B1 (es)
AU (1) AU2016339949B2 (es)
BR (1) BR112018007591A2 (es)
CA (1) CA3001570C (es)
CL (1) CL2018000961A1 (es)
MX (1) MX2018004519A (es)
SG (1) SG11201802954SA (es)
WO (1) WO2017066351A1 (es)

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EP3362179A4 (en) 2019-03-20
US20180230453A1 (en) 2018-08-16
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AU2016339949A1 (en) 2018-05-10
WO2017066351A1 (en) 2017-04-20
CL2018000961A1 (es) 2018-11-16
US20170107507A1 (en) 2017-04-20
US10526595B2 (en) 2020-01-07
CA3001570C (en) 2022-09-20
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SG11201802954SA (en) 2018-05-30

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