BR112017013934A2 - método para imobilização de um oligonucleotídeo identificado em um substrato polimérico não modificado, métodos para imobilização de uma molécula de interesse em um substrato polimérico não modificado, uso de uma identificação fixada a um oligonucleotídeo para imobilização do oligonucleotídeo identificado em um substrato polimérico não modificado por fisissorção, micromatrizes, e kit de diagnóstico - Google Patents

método para imobilização de um oligonucleotídeo identificado em um substrato polimérico não modificado, métodos para imobilização de uma molécula de interesse em um substrato polimérico não modificado, uso de uma identificação fixada a um oligonucleotídeo para imobilização do oligonucleotídeo identificado em um substrato polimérico não modificado por fisissorção, micromatrizes, e kit de diagnóstico

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Publication number
BR112017013934A2
BR112017013934A2 BR112017013934A BR112017013934A BR112017013934A2 BR 112017013934 A2 BR112017013934 A2 BR 112017013934A2 BR 112017013934 A BR112017013934 A BR 112017013934A BR 112017013934 A BR112017013934 A BR 112017013934A BR 112017013934 A2 BR112017013934 A2 BR 112017013934A2
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BR
Brazil
Prior art keywords
polymeric substrate
oligonucleotide
immobilization
identified
unmodified
Prior art date
Application number
BR112017013934A
Other languages
English (en)
Inventor
Martine Feitsma Harma
Lamberts Inge
Paulus Lambertus Kuijpers Steven
Original Assignee
Koninklijke Philips Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips Nv filed Critical Koninklijke Philips Nv
Publication of BR112017013934A2 publication Critical patent/BR112017013934A2/pt

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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

a presente invenção refere-se ao método para imobilização de um oligonucleotídeo identificado em um substrato polimérico não modificado, sendo que o método compreende as seguintes etapas: a) fornecer uma mistura que compreende líquido e um oligonucleotídeo identificado, b) aplicar a mistura da etapa a) em um substrato polimérico não modificado, sendo que o oligonucleotídeo é imobilizado no substrato polimérico não modificado através de fisissorção e micromatrizes obtidas por esse método. a invenção refere-se, adicionalmente, ao uso de uma identificação fixada a um oligonucleotídeo para imobilização do oligonucleotídeo identificado em um substrato polimérico não modificado por fisissorção. adicionalmente, a invenção refere-se ao uso das micromatrizes obtidas pelo método descrito no presente documento para ensaios e kits de diagnóstico que compreendem tais micromatrizes.
BR112017013934A 2014-12-31 2015-12-21 método para imobilização de um oligonucleotídeo identificado em um substrato polimérico não modificado, métodos para imobilização de uma molécula de interesse em um substrato polimérico não modificado, uso de uma identificação fixada a um oligonucleotídeo para imobilização do oligonucleotídeo identificado em um substrato polimérico não modificado por fisissorção, micromatrizes, e kit de diagnóstico BR112017013934A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14200710 2014-12-31
US201562104113P 2015-01-16 2015-01-16
PCT/EP2015/080813 WO2016107782A1 (en) 2014-12-31 2015-12-21 Modified oligonucleotide immobilization onto polymer substrate via physisorption

Publications (1)

Publication Number Publication Date
BR112017013934A2 true BR112017013934A2 (pt) 2018-02-20

Family

ID=52292726

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017013934A BR112017013934A2 (pt) 2014-12-31 2015-12-21 método para imobilização de um oligonucleotídeo identificado em um substrato polimérico não modificado, métodos para imobilização de uma molécula de interesse em um substrato polimérico não modificado, uso de uma identificação fixada a um oligonucleotídeo para imobilização do oligonucleotídeo identificado em um substrato polimérico não modificado por fisissorção, micromatrizes, e kit de diagnóstico

Country Status (7)

Country Link
US (1) US20170349942A1 (pt)
EP (1) EP3240630A1 (pt)
JP (1) JP2018501803A (pt)
CN (1) CN107109493A (pt)
BR (1) BR112017013934A2 (pt)
RU (1) RU2732791C2 (pt)
WO (1) WO2016107782A1 (pt)

Families Citing this family (1)

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Publication number Priority date Publication date Assignee Title
GB2580385B (en) * 2019-01-08 2022-10-12 Quantumdx Group Ltd Oligonucleotide deposition onto polypropylene substrates

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1232753B1 (en) * 1999-09-08 2008-03-19 Chugai Seiyaku Kabushiki Kaisha Stable protein solution filled in a container made from a hydrophobic resin and method of stabilizing the same
US6929944B2 (en) * 2001-08-31 2005-08-16 Beckman Coulter, Inc. Analysis using a distributed sample
JP4170082B2 (ja) * 2002-12-02 2008-10-22 住友ベークライト株式会社 マイクロアレイおよびその製造方法
JPWO2004089996A1 (ja) * 2003-04-04 2006-07-06 綜研化学株式会社 変性シクロオレフィンコポリマー、その製造方法及びそのポリマーの用途
RU2236467C1 (ru) * 2003-04-14 2004-09-20 Институт химической биологии и фундаментальной медицины СО РАН Способ получения днк-чипов
US7288316B2 (en) * 2003-11-21 2007-10-30 Topas Advanced Polymers, Inc. Cycloolefin copolymer heat sealable films
KR100580644B1 (ko) * 2004-02-16 2006-05-16 삼성전자주식회사 생물분자를 고체 기판상에 비공유적으로 고정화 하는 방법및 그에 의하여 제조되는 마이크로어레이
JP2006078197A (ja) * 2004-09-07 2006-03-23 Toray Ind Inc 選択結合性物質固定化担体
EP2473625B1 (en) * 2009-09-01 2018-03-07 Koninklijke Philips N.V. Devices and methods for microarray selection
US20120070836A1 (en) * 2010-06-08 2012-03-22 Millipore Corporation Methods of detecting residual amounts of polymers used in the purification of biomolecules
SG11201404892UA (en) * 2012-02-17 2014-09-26 Nvs Technologies Inc Polymer scaffolds for assay applications

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Publication number Publication date
WO2016107782A1 (en) 2016-07-07
US20170349942A1 (en) 2017-12-07
RU2017126986A3 (pt) 2019-05-23
RU2732791C2 (ru) 2020-09-22
EP3240630A1 (en) 2017-11-08
JP2018501803A (ja) 2018-01-25
RU2017126986A (ru) 2019-01-31
CN107109493A (zh) 2017-08-29

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