BR112017008194A2 - variantes de transportador de gal2 e seus usos - Google Patents

variantes de transportador de gal2 e seus usos

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Publication number
BR112017008194A2
BR112017008194A2 BR112017008194A BR112017008194A BR112017008194A2 BR 112017008194 A2 BR112017008194 A2 BR 112017008194A2 BR 112017008194 A BR112017008194 A BR 112017008194A BR 112017008194 A BR112017008194 A BR 112017008194A BR 112017008194 A2 BR112017008194 A2 BR 112017008194A2
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BR
Brazil
Prior art keywords
nucleic acid
present
acid molecules
polypeptides
host cells
Prior art date
Application number
BR112017008194A
Other languages
English (en)
Inventor
Farwick Alexander
Boles Eckhard
Kirchner Ferdinand
OREB Mislav
SCHADEWEG Virginia
Original Assignee
Johann Wolfgang Goethe Univ Frankfurt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johann Wolfgang Goethe Univ Frankfurt filed Critical Johann Wolfgang Goethe Univ Frankfurt
Publication of BR112017008194A2 publication Critical patent/BR112017008194A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/12—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing sulfite waste liquor or citrus waste
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01016—Ribulokinase (2.7.1.16)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017—Xylulokinase (2.7.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03004—L-Ribulose-5-phosphate 4-epimerase (5.1.3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01003—Arabinose isomerase (5.3.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

a presente invenção refere-se a polipeptídeos os quais são variantes de gal2 compreendendo no mínimo uma substituição de aminoácido em uma posição correspondente a m435, e opcionalmente uma ou mais substituições de aminoácidos adicionais. a presente invenção se refere adicionalmente a moléculas de ácido nucleico codificando para os polipeptídeos e a células hospedeiras contendo as moléculas de ácido nucleico referidas. a presente invenção se refere adicionalmente a um método para a produção de bioetanol e/ou outros compostos de base biológica, compreendendo a expressão das moléculas de ácido nucleico referidas, preferencialmente nas células hospedeiras referidas. a presente invenção também se refere à utilização dos polipeptídeos, das moléculas de ácido nucleico ou das células hospedeiras para a produção de bioetanol e;ou outros compostos de base biológica, e/ou para a fermentação recombinante de biomaterial contendo pentose(s), preferencialmente d-xilose e/ou l-arabinose
BR112017008194A 2014-10-22 2015-10-22 variantes de transportador de gal2 e seus usos BR112017008194A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14189927 2014-10-22
PCT/EP2015/074519 WO2016062821A1 (en) 2014-10-22 2015-10-22 Variants of gal2 transporter and their uses

Publications (1)

Publication Number Publication Date
BR112017008194A2 true BR112017008194A2 (pt) 2018-01-30

Family

ID=51798986

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017008194A BR112017008194A2 (pt) 2014-10-22 2015-10-22 variantes de transportador de gal2 e seus usos

Country Status (15)

Country Link
US (2) US10246495B2 (pt)
EP (2) EP3209678B1 (pt)
JP (1) JP6746570B2 (pt)
CN (2) CN107207572A (pt)
AU (1) AU2015334948B2 (pt)
BR (1) BR112017008194A2 (pt)
CA (2) CA2965385C (pt)
DK (1) DK3209677T3 (pt)
ES (1) ES2741836T3 (pt)
HU (1) HUE044424T2 (pt)
MX (1) MX2017005172A (pt)
MY (1) MY187130A (pt)
SI (1) SI3209678T1 (pt)
WO (2) WO2016062823A1 (pt)
ZA (1) ZA201702828B (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112020007140A2 (pt) * 2017-11-14 2020-09-29 Dsm Ip Assets B.V. polipeptídeos com especificidade de transporte de arabinose melhorada
WO2019185737A1 (en) * 2018-03-27 2019-10-03 Basf Se Xylose metabolizing yeast
CN109097375A (zh) * 2018-08-08 2018-12-28 淮阴师范学院 通过表达木糖转运蛋白来提高乳酸乳球菌利用木糖发酵乳酸效率的方法
CN110872596B (zh) * 2018-09-04 2021-08-24 中国科学院分子植物科学卓越创新中心 木糖阿拉伯糖共利用的酿酒酵母菌的构建方法
DK3690052T3 (da) * 2019-01-29 2023-06-19 Clariant Produkte Deutschland Gmbh Kimær promotor til anvendelse i metabolsk manipulation
CN113373073B (zh) * 2021-08-02 2022-08-12 山东大学 一种提高酿酒酵母菌株左旋葡聚糖转运能力及利用能力的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0202090D0 (sv) 2002-05-08 2002-07-04 Forskarpatent I Syd Ab A modifierd yeast consuming L-arabinose
DE102006060381B4 (de) 2006-12-20 2010-04-08 Johann Wolfgang Goethe-Universität Frankfurt am Main Neuer spezifischer Arabinose-Transporter aus der Hefe Pichia stipitis und dessen Verwendungen
WO2011059314A1 (en) * 2009-11-12 2011-05-19 Stichting Voor De Technische Wetenschappen Pentose transporters and uses thereof
MX341905B (es) * 2010-04-21 2016-09-06 Dsm Ip Assets B V * Celula apropiada para la fermentacion de una composicion de azucares mixtos.
KR20130100169A (ko) 2010-10-13 2013-09-09 디에스엠 아이피 어셋츠 비.브이. 펜토스 및 글루코스 발효 효모 세포

Also Published As

Publication number Publication date
EP3209677A1 (en) 2017-08-30
CA2965385C (en) 2023-11-07
BR112017007795A2 (pt) 2018-01-16
EP3209678A1 (en) 2017-08-30
US10246495B2 (en) 2019-04-02
AU2015334948A1 (en) 2017-03-16
JP2017536815A (ja) 2017-12-14
CA2960930A1 (en) 2016-04-28
CN107074918B (zh) 2021-07-30
HUE044424T2 (hu) 2019-10-28
AU2015334948B2 (en) 2019-07-11
US20170283835A1 (en) 2017-10-05
CN107074918A (zh) 2017-08-18
ES2741836T3 (es) 2020-02-12
SI3209678T1 (sl) 2019-07-31
CA2960930C (en) 2023-10-03
MX2017005172A (es) 2017-12-07
WO2016062823A1 (en) 2016-04-28
DK3209677T3 (da) 2020-08-03
EP3209677B1 (en) 2020-05-27
CA2965385A1 (en) 2016-04-28
US20170334955A1 (en) 2017-11-23
WO2016062821A1 (en) 2016-04-28
EP3209678B1 (en) 2019-05-01
JP6746570B2 (ja) 2020-08-26
US10308692B2 (en) 2019-06-04
MY187130A (en) 2021-09-02
ZA201702828B (en) 2020-01-29
CN107207572A (zh) 2017-09-26

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Owner name: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC (HU)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B09X Republication of the decision to grant [chapter 9.1.3 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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