BR112017001539A2 - Levedura recombinante, uso de levedura recombinante e método para produzir 2,3- butanodiol - Google Patents

Levedura recombinante, uso de levedura recombinante e método para produzir 2,3- butanodiol

Info

Publication number
BR112017001539A2
BR112017001539A2 BR112017001539A BR112017001539A BR112017001539A2 BR 112017001539 A2 BR112017001539 A2 BR 112017001539A2 BR 112017001539 A BR112017001539 A BR 112017001539A BR 112017001539 A BR112017001539 A BR 112017001539A BR 112017001539 A2 BR112017001539 A2 BR 112017001539A2
Authority
BR
Brazil
Prior art keywords
recombinant yeast
nucleic acids
acids encoding
butanodiol
produce
Prior art date
Application number
BR112017001539A
Other languages
English (en)
Other versions
BR112017001539B1 (pt
Inventor
Louis Dominique
Thomas Dominique
Jaillardon Karine
Bremond Mélanie
Original Assignee
Alderys
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alderys filed Critical Alderys
Publication of BR112017001539A2 publication Critical patent/BR112017001539A2/pt
Publication of BR112017001539B1 publication Critical patent/BR112017001539B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01004—R,R-butanediol dehydrogenase (1.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/03—Oxidoreductases acting on NADH or NADPH (1.6) with oxygen as acceptor (1.6.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01001—Pyruvate decarboxylase (4.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01005—Acetolactate decarboxylase (4.1.1.5)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

levedura recombinante, caracterizada pelo fato de que possui uma atividade de piruvato descarboxilase reduzida no genoma do qual foi inserida: - um ou mais ácidos nucleicos codificando uma síntese de acetolactato ou als, - um ou mais ácidos nucleicos codificando um acetolactato decarboxilase ou ald, - um ou mais ácidos nucleicos codificando um butanodiol desidrogenase ou bdh, e - uma ou mais cópias de ácidos nucleicos codificando um nadh oxidase ou noxe.
BR112017001539-0A 2014-07-25 2015-07-23 Levedura recombinante produtora de 2,3-butanodiol, uso desta e método para produzir 2,3-butanodiol BR112017001539B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14306202 2014-07-25
EP14306202.4 2014-07-25
PCT/EP2015/066920 WO2016012557A1 (en) 2014-07-25 2015-07-23 Microorganism strains for the production of 2.3- butanediol

Publications (2)

Publication Number Publication Date
BR112017001539A2 true BR112017001539A2 (pt) 2018-01-30
BR112017001539B1 BR112017001539B1 (pt) 2023-07-11

Family

ID=

Also Published As

Publication number Publication date
US20170166935A1 (en) 2017-06-15
ES2776361T3 (es) 2020-07-30
CA2956189A1 (en) 2016-01-28
US10619174B2 (en) 2020-04-14
EP3172330B1 (en) 2019-12-25
CA2956189C (en) 2021-11-23
EP3172330A1 (en) 2017-05-31
WO2016012557A1 (en) 2016-01-28

Similar Documents

Publication Publication Date Title
EA201990651A1 (ru) Распределяемые системы для эффективного продуцирования и применения композиций на основе микроорганизмов
BR112017019057A2 (pt) ?variantes de piruvato desidrogenase, um micro- organismo que compreende as mesmas e um método para produzir l-aminoácido com o uso das mesmas?
BR112016023707A2 (pt) método para produzir l-aminoácidos com o uso de uma bactéria alcalifílica
UY35379A (es) ?métodos y composiciones para el control de malezas?.
CL2016002169A1 (es) Inhibidores de diacilglicerol aciltransferasa 2 para uso en el tratamiento de trastornos metabólicos y relacionados.
CL2017000750A1 (es) Pienso de alta resistencia a la abrasión y de buena estabilidad en agua, que contiene pufa
BR112016029855A2 (pt) métodos de inoculação de leveduras para fermentação, e métodos e sistemas relacionados à fermentação
BR112015032121A2 (pt) Mutante de subunidade beta-prime (subunidade b') de rna polimerase, microrganismo do gênero corynebacterium e método para produzir l-lisina
BR112017016897A2 (pt) cepas fúngicas e métodos de uso
BR112016006048A8 (pt) composições antimicrobianas
EP3369821A4 (en) CORYNEBACTERIUM SP.- MICROORGANISM WITH L-ISOLEUCIN-PRODUCING SKILL AND METHOD FOR THE PRODUCTION OF L-ISOLEUCIN THEREWITH
BR112014000542A2 (pt) composições de peroxidase eosinofílica e métodos de uso das mesmas
EA201791911A1 (ru) Применение трихотецен-превращающей алкогольдегидрогеназы, способ превращения трихотеценов и трихотецен-превращающая добавка
BR112018074283A2 (pt) composição e métodos para terapia com microbiota.
BR112017017071A2 (pt) nova lisina descarboxilase, e método para produzir cadaverina utilizando a mesma
BR112016002105A2 (pt) Microrganismo modificado, método para produzir alanina e uso de um microrganismo modificado
MY171648A (en) Microorganism producing l-lysine and method for producing l-lysine using the same
BR112016025791A2 (pt) microrganismo modificado, método para produzir um composto orgânico e uso de um microrganismo modificado
WO2015160349A3 (en) Generation of document editors having functions specified by role policies
BR112017024855A2 (pt) métodos de produção de aldc
MX2018000502A (es) Mejora del metabolismo de microalga de xilosa.
BR112016027437A2 (pt) genes causadores que conferem tolerância ao ácido acético em leveduras
BR112016018195A2 (pt) micro-organismo modificado, método de produção de um composto orgânico e uso de um micro-organismo modificado
AR110615A1 (es) Gen alkb mutante
BR112017001539A2 (pt) Levedura recombinante, uso de levedura recombinante e método para produzir 2,3- butanodiol

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 23/07/2015, OBSERVADAS AS CONDICOES LEGAIS