AR110615A1 - Gen alkb mutante - Google Patents

Gen alkb mutante

Info

Publication number
AR110615A1
AR110615A1 ARP170101848A ARP170101848A AR110615A1 AR 110615 A1 AR110615 A1 AR 110615A1 AR P170101848 A ARP170101848 A AR P170101848A AR P170101848 A ARP170101848 A AR P170101848A AR 110615 A1 AR110615 A1 AR 110615A1
Authority
AR
Argentina
Prior art keywords
alkb
muting
gen
enzyme
point mutation
Prior art date
Application number
ARP170101848A
Other languages
English (en)
Inventor
Haas Thomas Dr
Wessel Mirja Dr
Schorsch Christoph Dr
Schaffer Steffen Dr
Ludwig Kirmair
Skerra Arne Prof
Original Assignee
Evonik Degussa Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa Gmbh filed Critical Evonik Degussa Gmbh
Publication of AR110615A1 publication Critical patent/AR110615A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11033—DNA oxidative demethylase (1.14.11.33)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15003—Alkane 1-monooxygenase (1.14.15.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se proporciona una célula microbiana que expresa una enzima AlkB (oxidorreductasa dependiente de rubredoxina) mutante, comprendiendo la enzima AlkB mutante por lo menos una mutación puntual en la secuencia del-tipo salvaje de AlkB, donde la mutación puntual está en la posición de aminoácido V129 y/o T136 de la enzima AlkB del tipo salvaje. Se proporciona además un método para producir ácido w-hidroxicarboxílico y/o éster del mismo utilizando esta célula.
ARP170101848A 2016-07-04 2017-07-04 Gen alkb mutante AR110615A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP16177711.5A EP3336180A1 (en) 2016-07-04 2016-07-04 Mutant alkb gene

Publications (1)

Publication Number Publication Date
AR110615A1 true AR110615A1 (es) 2019-04-17

Family

ID=56368852

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP170101848A AR110615A1 (es) 2016-07-04 2017-07-04 Gen alkb mutante

Country Status (10)

Country Link
US (1) US11034938B2 (es)
EP (2) EP3336180A1 (es)
JP (1) JP7063827B2 (es)
KR (1) KR102375464B1 (es)
CN (1) CN109689863B (es)
AR (1) AR110615A1 (es)
BR (1) BR112019000198A2 (es)
ES (1) ES2901993T3 (es)
SG (2) SG10202100044PA (es)
WO (1) WO2018007289A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108913671B (zh) * 2018-06-15 2020-07-24 浙江科技学院 一种ω-转氨酶突变体及其应用
CN114107155B (zh) * 2021-11-29 2023-07-04 安徽大学 一种中长链烷烃诱导型生物传感器及其应用
CN116949152A (zh) * 2023-06-16 2023-10-27 广州国家实验室 一种单碱基水平检测基因组DNA上N4-mC及5mC修饰的测序方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007060705A1 (de) * 2007-12-17 2009-06-18 Evonik Degussa Gmbh ω-Aminocarbonsäuren oder ihre Lactame, herstellende, rekombinante Zellen
US8309333B1 (en) * 2009-11-16 2012-11-13 The United States of America, as represented by Department of Energy Regioselective alkane hydroxylation with a mutant AlkB enzyme
CN105316303B (zh) * 2009-11-20 2019-05-31 丹尼斯科美国公司 具有改善的特性的β-葡萄糖苷酶I变体
DE102010015807A1 (de) 2010-04-20 2011-10-20 Evonik Degussa Gmbh Biokatalytisches Oxidationsverfahren mit alkL-Genprodukt
DE102011110946A1 (de) 2011-08-15 2016-01-21 Evonik Degussa Gmbh Biotechnologisches Syntheseverfahren von omegafunktionalisierten Carbonsäuren und Carbonsäure-Estern aus einfachen Kohlenstoffquellen
EP2602328A1 (de) * 2011-12-05 2013-06-12 Evonik Industries AG Verfahren zur Oxidation von Alkanen unter Verwendung einer AlkB Alkan 1-Monooxygenase
EP2607490A1 (de) 2011-12-22 2013-06-26 Evonik Industries AG Verfahren zur verbesserten Abtrennung einer hydrophoben organischen Lösung von einem wässrigen Kulturmedium
EP2730655A1 (de) 2012-11-12 2014-05-14 Evonik Industries AG Verfahren zur Umsetzung eines Carbonsäureesters unter Verwendung BioH-defizienter Zellen
EP2746397A1 (de) * 2012-12-21 2014-06-25 Evonik Industries AG Herstellung von Omega-Aminofettsäuren
EP2759598A1 (de) * 2013-01-24 2014-07-30 Evonik Industries AG Verfahren zur Herstellung von alpha, omega-Alkandiol
EP2949214A1 (en) * 2014-05-26 2015-12-02 Evonik Degussa GmbH Methods of producing rhamnolipids

Also Published As

Publication number Publication date
KR102375464B1 (ko) 2022-03-18
SG11201811569SA (en) 2019-01-30
CN109689863A (zh) 2019-04-26
CN109689863B (zh) 2022-10-04
EP3478826B1 (en) 2021-11-17
SG10202100044PA (en) 2021-02-25
US11034938B2 (en) 2021-06-15
EP3478826A1 (en) 2019-05-08
ES2901993T3 (es) 2022-03-24
WO2018007289A1 (en) 2018-01-11
JP2019519237A (ja) 2019-07-11
JP7063827B2 (ja) 2022-05-09
EP3336180A1 (en) 2018-06-20
BR112019000198A2 (pt) 2019-04-24
KR20190025955A (ko) 2019-03-12
US20190153405A1 (en) 2019-05-23

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