BR112016020709A2 - variantes de hppd e métodos de uso - Google Patents

variantes de hppd e métodos de uso

Info

Publication number
BR112016020709A2
BR112016020709A2 BR112016020709A BR112016020709A BR112016020709A2 BR 112016020709 A2 BR112016020709 A2 BR 112016020709A2 BR 112016020709 A BR112016020709 A BR 112016020709A BR 112016020709 A BR112016020709 A BR 112016020709A BR 112016020709 A2 BR112016020709 A2 BR 112016020709A2
Authority
BR
Brazil
Prior art keywords
hppd
inhibitors
slow
binding
enzymes
Prior art date
Application number
BR112016020709A
Other languages
English (en)
Inventor
Laber Bernd
Poree Fabien
Lange Gudrun
Dubald Manuel
Armstrong Roxanne
Original Assignee
Bayer Cropscience Ag
Bayer Cropscience Lp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Cropscience Ag, Bayer Cropscience Lp filed Critical Bayer Cropscience Ag
Publication of BR112016020709A2 publication Critical patent/BR112016020709A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/713—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with four or more nitrogen atoms as the only ring hetero atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11001—Catechol 1,2-dioxygenase (1.13.11.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11027—4-Hydroxyphenylpyruvate dioxygenase (1.13.11.27)

Abstract

na presente invenção, são descritas enzimas hppd e plantas que as contêm que mostram uma tolerância total contra diversas classes de inibidores de hppd. um conjunto de enzimas hppd foi projetado, as quais não têm nenhuma afinidade ou têm apenas uma afinidade significativamente reduzida a inibidores de hppd e, ao mesmo tempo, a taxa de dissociação dos inibidores de hppd da enzima é aumentada a tal ponto que os inibidores de hppd não atuem mais como inibidores de ligação lenta ou de ligação forte lenta, porém, em vez disso, se tornem inibidores totalmente reversíveis. em particular, polinucleotídeos isolados que codificam polipeptídeos com tolerância a inibidor de hppd são fornecidos. adicionalmente, sequências de aminoácido correspondentes aos polinucleotídeos são abrangidas.
BR112016020709A 2014-03-11 2015-03-09 variantes de hppd e métodos de uso BR112016020709A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461951039P 2014-03-11 2014-03-11
EP14159634 2014-03-13
PCT/EP2015/054858 WO2015135881A1 (en) 2014-03-11 2015-03-09 Hppd variants and methods of use

Publications (1)

Publication Number Publication Date
BR112016020709A2 true BR112016020709A2 (pt) 2017-10-10

Family

ID=50277022

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016020709A BR112016020709A2 (pt) 2014-03-11 2015-03-09 variantes de hppd e métodos de uso

Country Status (12)

Country Link
US (2) US10400249B2 (pt)
EP (1) EP3116992B1 (pt)
JP (1) JP2017514457A (pt)
KR (1) KR20160132027A (pt)
CN (1) CN107075488A (pt)
AU (1) AU2015228963A1 (pt)
BR (1) BR112016020709A2 (pt)
CA (1) CA2941905A1 (pt)
EA (1) EA201691811A1 (pt)
MX (1) MX360999B (pt)
WO (1) WO2015135881A1 (pt)
ZA (1) ZA201605639B (pt)

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KR20150070148A (ko) 2012-09-14 2015-06-24 바이엘 크롭사이언스 엘피 Hppd 변이체들 및 사용 방법들
CA2931549A1 (en) * 2013-11-28 2015-06-04 Bayer Cropscience Aktiengesellschaft Use of 2-chloro-3-(methylsulfanyl)-n-(1-methyl-1h-tetrazol-5-yl)-4-(trifluoromethyl)benzamide or its salts for controlling unwanted plants in areas of transgenic crop plants being tolerant to hppd inhibitor herbicides
CA2942171C (en) 2014-03-11 2023-05-09 Bayer Cropscience Lp Hppd variants and methods of use
CN105567706A (zh) * 2015-12-28 2016-05-11 北京未名凯拓作物设计中心有限公司 一种来源于恶臭假单胞菌的抗hppd抑制剂基因及其应用
CN105567707A (zh) * 2015-12-28 2016-05-11 北京未名凯拓作物设计中心有限公司 一种抗hppd抑制剂基因及其应用
CN105567705A (zh) * 2015-12-28 2016-05-11 北京未名凯拓作物设计中心有限公司 一种来源于人苍白杆菌的抗hppd抑制剂基因及其应用
CN105543245A (zh) * 2015-12-28 2016-05-04 北京未名凯拓作物设计中心有限公司 一种来源于铜绿假单胞菌的抗hppd抑制剂基因及其应用
MX2019007593A (es) 2016-12-22 2019-12-02 BASF Agricultural Solutions Seed US LLC Evento de elíte ee-gm5 y métodos y kits para identificar dicho evento en muestras biológicas.
BR112019012947A2 (pt) 2016-12-22 2019-11-26 BASF Agricultural Solutions Seed US LLC moléculas de ácido nucleico, dna, plantas, produto de soja, métodos para produção de um produto de soja, para proteção de plantas, para produção de uma planta, para identificação, pares de iniciadores, para confirmação da pureza de sementes, para determinação do estado de zigosidade, para redução da perda de rendimento e para aumento do rendimento de plantas, processo para controle de ervas daninhas, uso, sondas, estojo e método de detecção da presença do evento de elite
WO2018162330A1 (en) * 2017-03-07 2018-09-13 Bayer Cropscience Aktiengesellschaft Hppd variants and methods of use
WO2018162329A1 (en) * 2017-03-07 2018-09-13 Bayer Cropscience Aktiengesellschaft Hppd variants and methods of use
BR112019018175A2 (pt) 2017-03-07 2020-04-07 BASF Agricultural Solutions Seed US LLC molécula, célula, planta, sementes, polipeptídeos recombinantes, método para produzir um polipeptídeo, planta, método para controlar ervas, uso do ácido nucleico e produto de utilidade
BR112019018056A2 (pt) * 2017-03-07 2020-08-11 BASF Agricultural Solutions Seed US LLC molécula de ácido nucleico recombinante, cassete de expressão, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, métodos para conferir tolerância e para controlar ervas daninhas, produto de utilidade e uso da sequência de nucleotídeos
US11371056B2 (en) 2017-03-07 2022-06-28 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
CN107794263B (zh) * 2017-09-30 2019-10-11 河北大学 一种提高黑色素产率的基因及其应用
WO2019083810A1 (en) * 2017-10-24 2019-05-02 Basf Se IMPROVING HERBICIDE TOLERANCE FOR 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) INHIBITORS BY NEGATIVE REGULATION OF HPPD EXPRESSION IN SOYBEANS
CN112673096A (zh) * 2018-05-25 2021-04-16 巴斯夫农业种子解决方案美国有限责任公司 含有优良事件ee-gm4的植物，和用于在生物样品中识别所述事件的方法和试剂盒，以及其处理
US20210207161A1 (en) * 2018-05-25 2021-07-08 BASF Agricultural Solutions Seed US LLC Plants containing elite event ee-gm5 and methods and kits for identifying such event in biological samples, and treatment thereof
CN113403289B (zh) * 2018-06-04 2023-05-23 青岛清原种子科学有限公司 突变型对羟苯基丙酮酸双氧化酶、其编码核酸以及应用
WO2019233863A1 (de) 2018-06-04 2019-12-12 Bayer Aktiengesellschaft Herbizid wirksame bizyklische benzoylpyrazole
WO2021051265A1 (zh) * 2019-09-17 2021-03-25 北京大北农生物技术有限公司 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途
CN111334484B (zh) * 2020-03-19 2022-06-03 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途
EP4250927A1 (en) * 2020-11-30 2023-10-04 Syngenta Crop Protection AG Novel hydroxyphenylpyruvate dioxygenase polypeptides and methods of use thereof
CN114585731A (zh) * 2021-12-15 2022-06-03 北京大北农生物技术有限公司 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途

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EP2389442A4 (en) 2009-01-22 2012-07-04 Syngenta Participations Ag MUTANT HYDROXYPHENYLPYRUVATE DIOXYGENASE POLYPEPTIDES AND METHODS OF USE
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US20130053243A1 (en) * 2010-05-04 2013-02-28 Basf Se Plants having increased tolerance to herbicides
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EP2453012B1 (en) 2010-11-10 2016-06-01 Bayer CropScience AG HPPD variants and methods of use
WO2013064964A1 (en) * 2011-11-02 2013-05-10 Basf Se Plants having increased tolerance to herbicides

Also Published As

Publication number Publication date
KR20160132027A (ko) 2016-11-16
ZA201605639B (en) 2019-01-30
EA201691811A1 (ru) 2017-05-31
US10400249B2 (en) 2019-09-03
AU2015228963A1 (en) 2016-09-01
MX2016011752A (es) 2016-12-12
WO2015135881A1 (en) 2015-09-17
EP3116992B1 (en) 2019-06-12
EP3116992A1 (en) 2017-01-18
MX360999B (es) 2018-11-23
CN107075488A (zh) 2017-08-18
JP2017514457A (ja) 2017-06-08
CA2941905A1 (en) 2015-09-17
US20200002715A1 (en) 2020-01-02
US20170016018A1 (en) 2017-01-19

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B25A Requested transfer of rights approved

Owner name: BAYER CROPSCIENCE AKTIENGESELLSCHAFT (DE) ; BASF SE (DE)

B25A Requested transfer of rights approved

Owner name: BAYER CROPSCIENCE AKTIENGESELLSCHAFT (DE) ; BASF AGRICULTURAL SOLUTIONS SEED US LLC (US)

B25A Requested transfer of rights approved

Owner name: BASF AGRICULTURAL SOLUTIONS SEED US LLC (US)

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]