CN114585731A - 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途 - Google Patents
突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途 Download PDFInfo
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Abstract
本发明涉及一种突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途，所述突变的羟基苯丙酮酸双加氧酶多肽保留催化对羟基苯丙酮酸转化为尿黑酸或尿黑酸盐的活性，且对HPPD抑制剂除草剂的敏感性低于野生型HPPD，其在对应于SEQ ID NO：1所示的氨基酸序列包含如下位点处的氨基酸突变：在F372位被A、G或V取代且在F383位被W取代。本发明首次公开了对不同物种来源的羟基苯丙酮酸双加氧酶多肽的372位点+383位点进行组合突变可以赋予植物对HPPD抑制剂类除草剂表现出协同增效的耐受性，在植物上应用前景广阔。
Description
技术领域
本发明涉及一种突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途，特别是涉及一种对HPPD抑制剂除草剂具有耐受性的突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途。
背景技术
羟基苯丙酮酸双加氧酶(hydroxyphenylpyruvate dioxygenase，简称HPPD)是一种酶，其在铁离子(Fe2+)和氧的存在下，催化酪氨酸的降解产物-对羟基苯丙酮酸(hydroxyphenylpyruvic acid，简称HPP)转化为植物中生育酚和质体醌(plastoquinone，简称PQ)的前体-尿黑酸盐/尿黑酸(homogentisate，简称HG)的反应。生育酚具有膜相关抗氧化剂的作用；PQ不仅是PS II和细胞色素b6/f复合物之间的电子载体，而且是类胡萝卜素生物合成中八氢番茄红素去饱和酶的必需辅因子。
通过抑制HPPD而发挥作用的除草剂主要有三酮类、异噁唑类和吡唑啉酮类三个化学家族。在植物中，它们通过抑制HPPD阻断了酪氨酸生物合成PQ，从而导致PQ耗尽，类胡萝卜素缺乏。上述抑制HPPD的除草剂是植物韧皮部可移动的漂白剂，其可以引起暴露于光的新的分生组织和叶片显现出白色，而类胡萝卜素对于光防护是必需的，在缺乏类胡萝卜素的情况下，紫外线辐射和活性氧中间体会破坏叶绿素的合成和功能，从而导致植物生长受到抑制，甚至死亡。
用于提供耐受HPPD抑制剂除草剂植物的方法主要包括：1)将HPPD过量表达从而在植物中产生大量的HPPD，尽管存在HPPD抑制剂除草剂，但这些HPPD与该HPPD抑制剂除草剂是充分作用的，从而具有足够的可供使用的功能性酶。2)将目标HPPD突变成一种功能性HPPD，该功能性HPPD对于除草剂或其活性代谢物是较不敏感的，但其保留了转化为HG的性质。现有技术已经报道了几种突变体HPPD多肽，其相对于它们从其衍生的初始野生型序列在一个或多个位置处具有氨基酸突变，且展示出对于一种或多种HPPD抑制剂除草剂增强的耐受性。且现有技术进一步报道了将两个氨基酸突变进行组合后获得的突变体HPPD多肽对HPPD抑制剂除草剂的耐受性优于仅具有两个氨基酸突变中的任意一个氨基酸突变的HPPD多肽对HPPD抑制剂除草剂的耐受性。然而，至今尚无关于不同物种来源的HPPD多肽在对应于燕麦野生型HPPD多肽的372位点和383位点进行组合突变可以赋予植物对HPPD抑制剂类除草剂表现出协同增效的耐受性的报道。
发明内容
本发明的目的是提供一种新的突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途，所述突变的羟基苯丙酮酸双加氧酶多肽不仅具有HPPD酶活性，而且使得转入所述突变的羟基苯丙酮酸双加氧酶多肽编码基因的植物对HPPD抑制剂除草剂表现出协同增效的耐受性。
为实现上述目的，本发明提供了一种突变的羟基苯丙酮酸双加氧酶多肽，其保留催化对羟基苯丙酮酸转化为尿黑酸或尿黑酸盐的活性，且对HPPD抑制剂除草剂的敏感性低于野生型HPPD，其在对应于SEQ ID NO：1所示的氨基酸序列包含如下位点处的氨基酸突变：在F372位被A、G或V取代且在F383位被W取代；
优选地，所述突变的羟基苯丙酮酸双加氧酶多肽在对应于SEQ ID NO：1所示的氨基酸序列包含如下位点处的氨基酸突变：在F372位被A取代且在F383位被W取代。
在上述技术方案的基础上，所述突变的羟基苯丙酮酸双加氧酶多肽包含第二突变；
优选地，所述第二突变在对应于SEQ ID NO：1所示的氨基酸包含如下至少一个位点处的氨基酸突变：A106G、A107缺失、A111T或K351N。
具体地，所述突变的羟基苯丙酮酸双加氧酶多肽包括：具有SEQ ID NO：173、SEQID NO：182、SEQ ID NO：185、SEQ ID NO：188、SEQ ID NO：191、SEQ ID NO：194、SEQ ID NO：197、SEQ ID NO：200或SEQ ID NO：203所示的氨基酸序列组成的多肽。
进一步地，所述突变的羟基苯丙酮酸双加氧酶多肽衍生自植物野生型HPPD或微生物野生型HPPD；
优选地，所述植物包括单子叶植物和双子叶植物；更优选地，所述植物为燕麦、小麦、大麦、谷子、玉米、高粱、二穗短柄草、水稻、烟草、向日葵、苜蓿、大豆、鹰嘴豆、花生、甜菜、黄瓜、棉花、油菜、土豆、番茄或拟南芥；
优选地，所述微生物为荧光假单胞菌。
为实现上述目的，本发明还提供了一种编码所述突变的羟基苯丙酮酸双加氧酶多肽的多核苷酸。
为实现上述目的，本发明还提供了表达盒或重组载体，包含在有效连接的调控序列调控下的所述多核苷酸。
为实现上述目的，本发明还提供了一种扩展植物耐受的除草剂范围的方法，包括：将所述突变的羟基苯丙酮酸双加氧酶多肽与至少一种不同于所述突变的羟基苯丙酮酸双加氧酶多肽的除草剂耐受性蛋白质一起表达。
进一步地，所述除草剂耐受性蛋白质为5-烯醇丙酮酰莽草酸-3-磷酸合酶、草甘膦氧化还原酶、草甘膦-N-乙酰转移酶、草甘膦脱羧酶、草铵膦乙酰转移酶、α酮戊二酸依赖性双加氧酶、麦草畏单加氧酶、乙酰乳酸合酶、细胞色素类蛋白质和/或原卟啉原氧化酶。
为实现上述目的，本发明还提供了一种选择转化的植物细胞的方法，包括：用所述多核苷酸转化多个植物细胞，并在允许表达所述多核苷酸的转化细胞生长，而杀死未转化细胞或抑制未转化细胞生长的HPPD抑制剂除草剂浓度下培养所述细胞；
优选地，所述植物包括单子叶植物和双子叶植物；更优选地，所述植物为燕麦、小麦、大麦、谷子、高粱、二穗短柄草、水稻、烟草、向日葵、苜蓿、大豆、鹰嘴豆、花生、甜菜、黄瓜、棉花、油菜、土豆、番茄或拟南芥；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮；具体优选地，选择转化的大豆植物细胞的方法包括：用所述多核苷酸转化多个大豆植物细胞，并在允许表达所述多核苷酸的转化细胞生长，而杀死未转化细胞或抑制未转化细胞生长的HPPD抑制剂除草剂浓度下培养所述细胞，所述HPPD抑制剂除草剂为苯吡唑草酮、甲基磺草酮或异噁唑草酮。
为实现上述目的，本发明还提供了一种控制杂草的方法，包括：对种植目的植物的大田施用有效剂量的HPPD抑制剂除草剂，所述目的植物包含所述多核苷酸；
优选地，所述目的植物包括单子叶植物和双子叶植物；更优选地，所述目的植物为燕麦、小麦、大麦、谷子、高粱、二穗短柄草、水稻、烟草、向日葵、苜蓿、大豆、鹰嘴豆、花生、甜菜、黄瓜、棉花、油菜、土豆、番茄或拟南芥；进一步优选地，所述目的植物为草甘膦耐受性植物，所述杂草为草甘膦抗性杂草；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮；具体优选地，控制杂草的方法包括：对种植大豆植物的大田施用有效剂量的HPPD抑制剂除草剂，所述大豆植物包含所述多核苷酸，所述HPPD抑制剂除草剂为苯吡唑草酮、甲基磺草酮或异噁唑草酮。
为实现上述目的，本发明还提供了一种用于保护植物免受由HPPD抑制剂除草剂引起的损伤或赋予植物HPPD抑制剂除草剂耐受性的方法，包括：将所述多核苷酸或所述表达盒或所述重组载体导入植物，使导入后的植物产生足够保护其免受HPPD抑制剂除草剂损害量的所述突变的羟基苯丙酮酸双加氧酶多肽；
优选地，所述植物包括单子叶植物和双子叶植物；更优选地，所述植物为燕麦、小麦、大麦、谷子、高粱、二穗短柄草、水稻、烟草、向日葵、苜蓿、大豆、鹰嘴豆、花生、甜菜、黄瓜、棉花、油菜、土豆、番茄或拟南芥；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮；具体优选地，用于保护大豆植物免受由HPPD抑制剂除草剂引起的损伤或赋予大豆植物HPPD抑制剂除草剂耐受性的方法包括：将所述多核苷酸或所述表达盒或所述重组载体导入大豆植物，使导入后的大豆植物产生足够保护其免受HPPD抑制剂除草剂损害量的所述突变的羟基苯丙酮酸双加氧酶多肽，所述HPPD抑制剂除草剂为苯吡唑草酮、甲基磺草酮或异噁唑草酮。
为实现上述目的，本发明还提供了一种产生耐受HPPD抑制剂除草剂植物的方法，包括向植物的基因组中引入所述多核苷酸；
优选地，所述引入的方法包括遗传转化方法、基因组编辑方法或基因突变方法；
优选地，所述植物包括单子叶植物和双子叶植物；更优选地，所述植物为燕麦、小麦、大麦、谷子、高粱、二穗短柄草、水稻、烟草、向日葵、苜蓿、大豆、鹰嘴豆、花生、甜菜、黄瓜、棉花、油菜、土豆、番茄或拟南芥；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮；具体优选地，产生耐受HPPD抑制剂除草剂大豆植物的方法包括：向大豆植物的基因组中引入所述多核苷酸，所述HPPD抑制剂除草剂为苯吡唑草酮、甲基磺草酮或异噁唑草酮。
为实现上述目的，本发明还提供了一种培养耐受HPPD抑制剂除草剂植物的方法，包括：
种植至少一个植物繁殖体，所述植物繁殖体的基因组中包括所述多核苷酸；
使所述植物繁殖体长成植株；
将有效剂量的HPPD抑制剂除草剂施加到至少包含所述植株的植物生长环境中，收获与其他不具有所述多核苷酸的植株相比具有减弱的植物损伤和/或具有增加的植物产量的植株；
优选地，所述植物包括单子叶植物和双子叶植物；更优选地，所述植物为燕麦、小麦、大麦、谷子、高粱、二穗短柄草、水稻、烟草、向日葵、苜蓿、大豆、鹰嘴豆、花生、甜菜、黄瓜、棉花、油菜、土豆、番茄或拟南芥；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮；具体优选地，培养耐受HPPD抑制剂除草剂大豆植物的方法包括：种植至少一粒大豆植物种子，所述大豆植物种子的基因组中包括所述多核苷酸；使所述大豆植物种子长成大豆植株；将有效剂量的HPPD抑制剂除草剂施加到至少包含所述大豆植株的植物生长环境中，收获与其他不具有所述多核苷酸的大豆植株相比具有减弱的植物损伤和/或具有增加的植物产量的大豆植株，所述HPPD抑制剂除草剂为苯吡唑草酮、甲基磺草酮或异噁唑草酮。
本发明还提供了一种获得加工农产品的方法，包括将由所述方法获得的耐受HPPD抑制剂除草剂植物的收获物进行处理以获得加工农产品。
为实现上述目的，本发明还提供了一种控制杂草生长的种植系统，包括HPPD抑制剂除草剂和存在至少一种目的植物的植物生长环境，所述目的植物包含所述多核苷酸；
优选地，所述目的植物包括单子叶植物和双子叶植物；更优选地，所述目的植物为燕麦、小麦、大麦、谷子、高粱、二穗短柄草、水稻、烟草、向日葵、苜蓿、大豆、鹰嘴豆、花生、甜菜、黄瓜、棉花、油菜、土豆、番茄或拟南芥；进一步优选地，所述目的植物为草甘膦耐受性植物，所述杂草为草甘膦抗性杂草；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮；具体优选地，控制杂草生长的种植系统包括HPPD抑制剂除草剂和存在至少一种大豆植物的植物生长环境，所述大豆植物包含所述多核苷酸，所述HPPD抑制剂除草剂为苯吡唑草酮、异噁唑草酮或甲基磺草酮。
为实现上述目的，本发明还提供了一种所述突变的羟基苯丙酮酸双加氧酶多肽在赋予植物HPPD抑制剂除草剂耐受性中的用途；
优选地，所述植物包括单子叶植物和双子叶植物；更优选地，所述植物为燕麦、小麦、大麦、谷子、高粱、二穗短柄草、水稻、烟草、向日葵、苜蓿、大豆、鹰嘴豆、花生、甜菜、黄瓜、棉花、油菜、土豆、番茄或拟南芥；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮；具体优选地，所述突变的羟基苯丙酮酸双加氧酶多肽在赋予大豆植物HPPD抑制剂除草剂耐受性中的用途，所述HPPD抑制剂除草剂为苯吡唑草酮、异噁唑草酮或甲基磺草酮。
本发明中使用的冠词“一种”和“一个”是指一个(种)或多于一个(种)(即至少一个)。例如，“一种要素”表示一种或多种要素(元件)。此外，术语“包括”或其变体例如“包括了”或“包含”应被理解为是指包括一种所述要素、整数或步骤，或一组要素、整数或步骤，但是不排除任何其他要素、整数或步骤，或者成组的要素、整数或步骤。
本发明中，术语“羟基苯丙酮酸双加氧酶(HPPD)”与“4-羟基苯丙酮酸双加氧酶(4-HPPD)”以及“对羟基苯丙酮酸双加氧酶(p-HPPD)”是同义的。
术语“HPPD抑制剂除草剂”是指直接或间接地抑制HPPD的除草剂，这些除草剂是漂白剂。市场上最易买到的HPPD抑制剂除草剂属于以下三个化学家族之一：(1)三酮类，例如，磺草酮(sulcotrione)(即2-[2-氯-4-(甲磺酰基)苯甲酰基]-1，3-环己二酮)、甲基磺草酮(mesotrione)(即2-[4-(甲磺酰基)-2-硝基苯甲酰基]-1，3-环己二酮)、环磺酮(tembotrione)(即2-[2-氯-4-(甲磺酰基)-3-[(2，2，2-三氟乙氧基)甲基]苯甲酰基]-1，3-环己二酮)；(2)异噁唑类，例如，异噁唑草酮(isoxaflutole)(即5-环丙基-4-异噁唑基[2-(甲磺酰基)-4-(三氟甲基)苯基]甲酮)；(3)吡唑啉酮(pyrazolinate)类，例如，苯吡唑草酮(topramezone)(即[3-(4，5-二氢-3-异噁唑基)-2-甲基-4-(甲磺酰基)苯基](5-羟基-1-甲基吡唑-4-基)甲酮)、磺酰草吡唑(pyrasulfotole)(5-羟基-1，3-二甲基吡唑-4-基(2-甲磺酰-4-三氟甲基苯基)甲酮)。
本发明中所述苯吡唑草酮(topramezone)，也称苯唑草酮，是指[3-(4，5-二氢-3-异噁唑基)-2-甲基-4-(甲磺酰基)苯基](5-羟基-1-甲基吡唑-4-基)甲酮，为白色结晶状固体。属于吡唑啉酮(pyrazolinate)类苗后茎叶处理内吸传导型HPPD抑制剂除草剂，常用剂型为30％悬浮剂。苯吡唑草酮商业制剂如苞卫，可防治禾本科和阔叶杂草，每亩用制剂5.6-6.7g，可以有效防治的杂草，包括但不限于，马唐(鸡爪草)、稗草、牛筋草、野黍、狗尾草(谷莠子)、藜、蓼、青麻、苘麻、野苋、马齿苋、苍耳、龙葵。苞卫加入莠去津后有显著的增效作用，除了对上述杂草具有优异的防效外，还可以对恶性阔叶杂草如刺儿菜(小蓟)、苣荬菜、铁苋菜、鸭跖草(兰花菜)具有良好的防除效果，特别是能有效防除硝磺草酮防效不佳的狗尾草、马唐、牛筋草、野黍。
本发明中所述有效剂量苯吡唑草酮是指以25-200g ai/ha使用，包括25-50g ai/ha、50-100g ai/ha、100-150g ai/ha或150-200g ai/ha。
本发明中所述异噁唑草酮(isoxaflutole)，也称异噁氟草，是指5-环丙基-4-异噁唑基[2-(甲磺酰基)-4-(三氟甲基)苯基]甲酮，为白色至灰黄色固体。属于有机杂环异噁唑类选择性内吸型苗前HPPD抑制剂除草剂，主要经由杂草幼根吸收传导而起作用。异噁唑草酮主要用于旱作物田防除苘麻、藜、地肤、猪毛菜、龙葵、反枝苋、柳叶刺蓼、鬼针草、马齿苋、繁缕、香薷、苍耳、铁苋菜、水棘针、酸模叶蓼和婆婆纳等多种一年生阔叶杂草，同时对马唐、稗草、牛筋草、千金子、大狗尾草和狗尾草等部分一年生禾本科杂草也有较好的防效。
本发明中所述有效剂量异噁唑草酮是指以35-280g ai/ha使用，包括35-70g ai/ha、70-140g ai/ha、140-200g ai/ha或200-280g ai/ha。
本发明中所述甲基磺草酮(mesotrione)，是指2-[4-(甲磺酰基)-2-硝基苯甲酰基]-1，3-环己二酮，为褐色或淡黄色固体。属于三酮类选择性内吸传导型植物苗前和苗后HPPD抑制剂除草剂，其可被植物的叶和根吸收，向顶向下传输，使植物分生组织在3-5天后出现黄化症状随之引起枯斑，继而导致整株植物死亡。甲基磺草酮可用于植物芽前或芽后防除一年生阔叶杂草和若干禾本科杂草，其中，可防除的一年生阔叶杂草主要包括苍耳、苘麻、藜、苋、蓼、龙葵和三裂叶豚草等，可防除的禾本科杂草主要包括幼龄稗、马唐、狗尾草和臂形草等。
本发明中所述有效剂量甲基磺草酮是指以52.5-420g ai/ha使用，包括52.5-105gai/ha、105-210g ai/ha、210-300g ai/ha或300-420g ai/ha。
本发明中，术语“抗性”是可遗传的，并允许植物在除草剂对给定植物进行一般除草剂有效处理的情况下生长和繁殖。正如本领域技术人员所认可的，即使给定植物受到除草剂处理的一定程度损伤，如很少的坏死、溶解、萎黄或其它损伤，但至少没有在产量上有显著影响，植物仍可被认为“抗性”，也即给定植物具有的抵抗除草剂诱导的各种程度损伤的提高的能力，而在同样的除草剂剂量下一般导致相同基因型野生型植物损伤。本发明中术语“耐性”或“耐受性”比术语“抗性”更广泛，并包括“抗性”。
本发明中术语“赋予”是指向植物提供特征或性状，如除草剂耐受性和/或其它所希望的性状。
本发明中术语“异源的”是指来自另一个来源。在DNA的背景下，“异源的”是指任何外来的“非自身”DNA，包括来自相同种类的另一个植物的DNA。例如，在本发明中，可以利用转基因的方法在大豆植物中表达大豆HPPD基因，该大豆HPPD基因仍被认为“异源的”DNA。
本发明中术语“核酸”包括涉及的单链或双链形式的脱氧核糖核苷酸或核糖核苷酸聚合物，并且除非另有限制，包括具有野生型核苷酸的基本性质的已知类似物(例如肽核酸)，因为它们以一种类似于野生型存在的核苷酸的方式与单链核酸杂交。
本发明中，当术语“编码”或“编码的”用于一种特定核酸的上下文中时，其意指核酸包含必需的信息以指导核苷酸序列翻译成一种特定蛋白质。用来编码蛋白质的信息通过使用密码子来详细说明。编码蛋白质的核酸可以包含在核酸的翻译区内的非翻译序列(例如内含子)，或者可以缺少此类插入的非翻译序列(例如在eDNA中)。
本发明所述蛋白质能够利用本领域的熟知方法以不同的方式进行改变，包括氨基酸置换、缺失、平截以及插入。例如，突变体HPPD蛋白的氨基酸序列变体和片段可以通过在DNA中的突变来制备。用于诱导多核苷酸突变的方法是本领域中熟悉的，例如，Kunkel在1985年《美国科学院院刊》(Proc.Natl.Acad.Sci)中的第82卷488-492页中进行了描述，Kunkel等人在1987年《酶学方法》(Methods in Enzymol.)期刊中的第154卷154：367-382页中进行了描述，Walker and Gaastra eds在1987年的美国专利4873192及该专利中引用的参考文献中进行了描述。Dayhoff等人1978年在《natl.Biomed.Res.Found》中发表的蛋白质序列和结构图谱模型中有关于不影响目的蛋白质生物活性的适当氨基酸置换方法的描述。保守置换(如将一个氨基酸用具有相似特性的另一个氨基酸进行代换)可能是不影响目的蛋白质生物活性的最佳方法。
本发明中所述突变的羟基苯丙酮酸双加氧酶多肽及其变体和片段，具有HPPD酶活性并且在植物中赋予对于某些类别的HPPD抑制剂除草剂的耐受性。所述突变的羟基苯丙酮酸双加氧酶多肽相对于它们从其衍生的初始野生型序列在一个或多个位置处具有氨基酸变化，并且展示出对于一种或多种HPPD抑制剂除草剂增强的耐受性。相对于同样的未突变初始酶，展示出对于至少一种HPPD抑制剂除草剂增强的耐受性。
编码所述突变的羟基苯丙酮酸双加氧酶多肽的DNA序列用于提供本发明的植物、植物细胞以及种子，相比于同样表达未突变初始酶的相同植物，它们提供了对于一种或多种HPPD抑制剂除草剂增强的耐受性。
编码所述突变的羟基苯丙酮酸双加氧酶多肽的植物HPPD的基因对于产生耐受HPPD抑制剂除草剂的植物是有用的。如此修饰的植物HPPD基因特别适合在植物中表达，以便向植物赋予除草剂耐受性。
许多HPPD序列在本领域中是已知的并且通过对相应氨基酸进行替换、缺失和/或添加来产生突变体HPPD序列。本发明所述372位点和383位点均以SEQ ID NO：1所示燕麦属野生型HPPD氨基酸序列的第372位氨基酸位置或第383位氨基酸位置为标准计算，本发明所述突变的羟基苯丙酮酸双加氧酶多肽在对应于SEQ ID NO：1的372位点和383位点处的氨基酸发生组合突变，所述组合突变形式包含在F372位被A、G或V取代且在F383位被W取代，优选的突变形式为在F372位被A取代且在F383位被W取代。因此，一种已知的或推测的HPPD序列可以使用标准的序列比对工具与SEQ ID NO：1所示氨基酸序列进行比对，并且可以在该已知的或推测的HPPD序列中，对此处描述的相对于SEQ ID NO：1所示氨基酸序列的相应氨基酸进行替换或缺失。
本发明包括一种突变体HPPD多肽，该突变体HPPD多肽衍生自植物HPPD或微生物HPPD，同时该多肽具有HPPD酶活性并且至少包含相应于SEQ ID NO：1的氨基酸372位点和383位点的一种组合突变，任选地进一步与其它位点突变(该HPPD多肽存在相应位点)进行组合，例如，与以下一个或多个对应位点上的突变进行组合：燕麦HPPD氨基酸序列的A106G、A107缺失、A111T或K351N。在上述不同的实施方案中，所述372位点和383位点组合突变形式可以为F372A+F383W、F372G+F383W或F372V+F383W。372位点和383位点组合突变形式结合其它位点突变的组合突变形式包含F372A+F383W+A107缺失、F372A+F383W+A111T、F372A+F383W+A106G、F372A+F383W+A106G+K351N、F372A+F383W+A107缺失+K351N或F372A+F383W+A111T+K351N。进一步地，本发明包括一种突变体HPPD多肽，该突变体HPPD多肽衍生自(替换、缺失和/或添加)植物或微生物的不同物种或同一物种不同生态型HPPD，示例性的，同一物种不同生态型HPPD包括但不限于如下物种生态型HPPD：登录号为XP_003617391.2、AAX59006.1、XP_003617384.1、XP_013466115.1、AET00342.2或A0A396HWH5的不同生态型苜蓿HPPD；登录号为A0A0D2PWQ6、A0A2P5SI66、A0A0D2LWN1或A0A0D2N7F6的不同生态型棉花HPPD；登录号为VDC64417.1、CDY10210.1、AFB74208.1、XP_013695640.1、XP_013695641.1、RID40406.1、RTD48932.1、XP_009118533.1、XP_009119049.1、XP_013723237.1、AFB74218.1或AFB74207.1的不同生态型油菜HPPD；登录号为A5Z1N7、I1M6Z4、A0A088MGH9或I1M6Z5的不同生态型大豆HPPD；登录号为XP_009770088.1或XP_009587203.1的不同生态型烟草HPPD；登录号为A3A3J1、B8AIH6或A0A0E0G1W2的不同生态型水稻HPPD；登录号为BAJ86732.1、BAJ95714.1或F2E412的不同生态型大麦HPPD(上述登录号来自于GenBank数据库或UniProtKnowledgebase数据库)。
术语“位点372”或“372位点”或“372单位点”，不仅狭义地指SEQ ID NO：1所示氨基酸序列的第372位氨基酸(苯丙氨酸)，并且广义地包括一种已知的或推测的HPPD氨基酸序列可以使用标准的序列比对工具(如CLUSTAL软件等)与SEQ ID NO：1所示氨基酸序列进行比对，并且可以在该已知的或推测的HPPD氨基酸序列中，获得与SEQ ID NO：1所示氨基酸序列第372位氨基酸相对应的位置，其可能不是该HPPD氨基酸序列的第372位。
术语“位点383”或“383位点”或“383单位点”，不仅狭义地指SEQ ID NO：1所示氨基酸序列的第383位氨基酸(苯丙氨酸)，并且广义地包括一种已知的或推测的HPPD氨基酸序列可以使用标准的序列比对工具(如CLUSTAL软件等)与SEQ ID NO：1所示氨基酸序列进行比对，并且可以在该已知的或推测的HPPD氨基酸序列中，获得与SEQ ID NO：1所示氨基酸序列第383位氨基酸相对应的位置，其可能不是该HPPD氨基酸序列的第383位。
术语“位点415”或“415位点”或“415单位点”，不仅狭义地指SEQ ID NO：1所示氨基酸序列的第415位氨基酸(苯丙氨酸)，并且广义地包括一种已知的或推测的HPPD氨基酸序列可以使用标准的序列比对工具(如CLUSTAL软件等)与SEQ ID NO：1所示氨基酸序列进行比对，并且可以在该已知的或推测的HPPD氨基酸序列中，获得与SEQ ID NO：1所示氨基酸序列第415位氨基酸相对应的位置，其可能不是该HPPD氨基酸序列的第415位。
同理，对其他位点的解释同上对“位点372”或“372位点”的解释。
术语“对应于”是指不同物种或同一物种不同生态型来源的HPPD氨基酸序列使用标准的序列比对工具与SEQ ID NO：1所示氨基酸序列进行比对并获得与SEQ ID NO：1所示氨基酸序列特定位点氨基酸相对应的位置，如与SEQ ID NO：1所示氨基酸序列第372位氨基酸或第383位氨基酸相对应的位置。
术语“多肽”、“肽”以及“蛋白”在本发明中可互换地使用，指的是氨基酸残基的聚合物。这些术语应用于氨基酸残基的聚合物，所述氨基酸残基的聚合物中的一个或多个氨基酸残基是一个相应的野生型存在的氨基酸的一种人工化学类似物，以及野生型存在的氨基酸聚合物。本发明的多肽可以从一种本发明披露的核酸或通过使用标准分子生物学技术来产生。例如，本发明的一种截短的蛋白可以通过在一种适当的宿主细胞中表达本发明的一种重组核酸，或者可选择地通过结合离体方法(如蛋白酶消化和纯化)来产生。
因此，本发明还提供了包括编码突变体HPPD多肽的多核苷酸序列的核酸分子，所述突变体HPPD多肽及其变体和片段具有HPPD酶活性并且在植物中赋予对于某些类别的HPPD抑制剂除草剂的耐受性。总体上，本发明包括编码在此所述的任何突变体HPPD多肽的任何多核苷酸序列，连同编码相对于在此所述的突变体HPPD多肽具有一个或多个保守性氨基酸替换的HPPD多肽的任何多核苷酸序列。提供功能上相似的氨基酸保守性取代是本领域技术人员所熟知的，以下五组各自包含彼此保守性取代的氨基酸：脂肪族：甘氨酸(G)、丙氨酸(A)、缬氨酸(V)、亮氨酸(L)、异亮氨酸(I)；芳香族：苯丙氨酸(F)、酪氨酸(Y)、色氨酸(W)；含硫的：甲硫氨酸(M)、半胱氨酸(C)；碱性的：精氨酸(I)、赖氨酸(K)、组氨酸(H)；酸性的：天冬氨酸(D)、谷氨酸(E)、天冬酰胺(N)、谷氨酰胺(Q)。
在一个实施方案中，本发明提供了一种多核苷酸序列，该多核苷酸序列编码一种衍生自植物HPPD的氨基酸序列或微生物HPPD的氨基酸序列，同时该多肽具有HPPD酶活性并且至少包含相应于SEQ ID NO：1的氨基酸位置372位点和383位点的组合突变。
因此，具有HPPD抑制剂除草剂耐受性活性并在严格条件下与本发明编码所述突变的羟基苯丙酮酸双加氧酶多肽的基因杂交的序列包括在本发明中。示例性的，这些序列与本发明序列SEQ ID NO：11-12、SEQ ID NO：33-34、SEQ ID NO：45-46、SEQ ID NO：57-58、SEQID NO：69-70、SEQ ID NO：81-82、SEQ ID NO：93-94、SEQ ID NO：105-106、SEQ ID NO：117-118、SEQ ID NO：129-130、SEQ ID NO：141-142、SEQ ID NO：153-154、SEQ ID NO：159-160、SEQ ID NO：165-166、SEQ ID NO：174-175、SEQ ID NO：183-184、SEQ ID NO：186-187、SEQID NO：189-190、SEQ ID NO：192-193、SEQ ID NO：195-196、SEQ ID NO：198-199、SEQ IDNO：201-202、SEQ ID NO：204-205至少大约75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更大的序列同源性。
任何常规的核酸杂交或扩增方法都可以用于鉴定本发明突变的HPPD基因的存在。核酸分子或其片段在一定情况下能够与其他核酸分子进行特异性杂交。本发明中，如果两个核酸分子能形成反平行的双链核酸结构，就可以说这两个核酸分子彼此间能够进行特异性杂交。如果两个核酸分子显示出完全的互补性，则称其中一个核酸分子是另一个核酸分子的“互补物”。本发明中，当一个核酸分子的每一个核苷酸都与另一个核酸分子的对应核苷酸互补时，则称这两个核酸分子显示出“完全互补性”。如果两个核酸分子能够以足够的稳定性相互杂交从而使它们在至少常规的“低度严格”条件下退火且彼此结合，则称这两个核酸分子为“最低程度互补”。类似地，如果两个核酸分子能够以足够的稳定性相互杂交从而使它们在常规的“高度严格”条件下退火且彼此结合，则称这两个核酸分子具有“互补性”。从完全互补性中偏离是可以允许的，只要这种偏离不完全阻止两个分子形成双链结构。为了使一个核酸分子能够作为引物或探针，仅需保证其在序列上具有充分的互补性，以使得在所采用的特定溶剂和盐浓度下能形成稳定的双链结构。
本发明中，基本同源的序列是一段核酸分子，该核酸分子在高度严格条件下能够和相匹配的另一段核酸分子的互补链发生特异性杂交。促进DNA杂交的适合的严格条件，例如，大约在45℃条件下用6.0×氯化钠/柠檬酸钠(SSC)处理，然后在50℃条件下用2.0×SSC洗涤，这些条件对本领域技术人员是公知的。例如，在洗涤步骤中的盐浓度可以选自低度严格条件的约2.0×SSC、50℃到高度严格条件的约0.2×SSC、50℃。此外，洗涤步骤中的温度条件可以从低度严格条件的室温约22℃，升高到高度严格条件的约65℃。温度条件和盐浓度可以都发生改变，也可以其中一个保持不变而另一个变量发生改变。优选地，本发明所述严格条件可为在6×SSC、0.5％SDS溶液中，在65℃下与本发明突变的HPPD基因发生特异性杂交，然后用2×SSC、0.1％SDS和1×SSC、0.1％SDS各洗膜1次。
本发明中，术语“杂交”或“特异性杂交”是指一种分子在严格条件下仅可与特定的核苷酸序列结合、双链化或杂交，这是在该序列存在于一种复合混合物(例如，总细胞)DNA或RNA中时进行的。
由于遗传密码子的丰余性，多种不同的DNA序列可以编码相同的氨基酸序列。产生这些编码相同或基本相同的蛋白的可替代DNA序列正在本领域技术人员的技术水平内。这些不同的DNA序列包括在本发明的范围内。所述“基本上相同的”序列是指有氨基酸取代、缺失、添加或插入但实质上不影响除草剂耐受性活性的序列，亦包括保留除草剂耐受性活性的片段。
术语“功能活性”或“活性”在本发明中指本发明用途的蛋白质/酶(单独或与其它蛋白质组合)具有降解或减弱除草剂活性的能力。产生本发明蛋白质的植物优选产生“有效量”的蛋白质，从而在用除草剂处理植物时，蛋白质表达的水平足以给予植物对除草剂(若无特别说明则为一般用量)完全或部分的耐受性。可以以通常杀死靶植物的用量、正常的大田用量和浓度使用除草剂。优选地，本发明的植物细胞和植物被保护免受除草剂处理引起的生长抑制或损伤。本发明的转化植物和植物细胞优选具有HPPD抑制剂除草剂的耐受性，即转化的植物和植物细胞能在有效量的HPPD抑制剂除草剂存在下生长。
本发明中所述的基因和蛋白质不但包括特定的示例序列，还包括保存了所述特定示例的蛋白质的HPPD抑制剂除草剂耐受性活性特征的部分和/片段(包括与全长蛋白质相比在内和/或末端缺失)、变体、突变体、变体蛋白质、取代物(有替代氨基酸的蛋白质)、嵌合体和融合蛋白。
本发明术语“变体”意指实质上类似的序列。对于多核苷酸，一种变体包括在参比多核苷酸之内的一个或多个内部位点处的一个或多个核苷酸的缺失和/或添加和/或在突变体HPPD多核苷酸中的一个或多个位点处的一个或多个核苷酸的替换。本发明中术语“参比多核苷酸或多肽”对应地包括突变体HPPD核苷酸序列或氨基酸序列。本发明中术语“野生型多核苷酸或多肽”对应地包括野生型发生的核苷酸序列或氨基酸序列。对于多核苷酸，保守型变体包括编码本发明的这些突变体HPPD多肽之一的氨基酸序列(由于遗传密码的简并性)。如这些野生型发生的等位基因变体可以使用熟知的分子生物学技术，例如使用以下概述的聚合酶链式反应(PCR)以及杂交技术来鉴定。变体多核苷酸还包括合成衍生的多核苷酸，例如通过使用定点诱变产生的但是仍然编码本发明的一种突变体HPPD蛋白的序列。通常，本发明的一种特定的多核苷酸的变体将具有与该特定的多核苷酸至少大约75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更多的序列同源性，通过序列对比程序和参数来确定同源性。
本发明中“变体蛋白质”意指从一种参比蛋白通过在该突变体HPPD蛋白中的一个或多个内部位点处的一个或多个氨基酸的缺失或添加和/或在该突变体HPPD蛋白中的一个或多个位点处的一个或多个氨基酸的替换而衍生的一种蛋白质。由本发明涵盖的变体蛋白是生物学活性的，即它们继续具有突变体HPPD蛋白的所希望的活性，即，仍具有所述的HPPD酶活性和/或除草剂耐性。此类变体可以产生于例如遗传多态性或产生于人工操作。本发明的一种突变体HPPD蛋白的生物活性变体将具有与该突变体HPPD蛋白的氨基酸序列的全部的至少大约85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更多的序列同源性，此同源性通过序列比对程序和参数所确定。本发明的一种蛋白质的一种生物活性变体可能不同于以少至1-15个氨基酸残基、少至1-10个(如6-10个)、少至5个(如4、3、2个、或甚至1个)氨基酸残基的蛋白质。
用于序列对比的方法在本领域中是熟知的并且可以使用数学算法来完成，这些数学算法如Myers and Miller(1988)CABIOS 4：11-17的算法；Smith et al.(1981)Adv.Appl.Math.2：482的局部比对算法；Need eman and Wunsch(1970)J.Mol.Biol.48：443-453的全局比对算法；以及Karlin and Altschul(1990)Proc.Natl.Acad.Sci.USA872264的算法，如在Karlin and Altschul(1993)Proc.Natl.Acad.Sci.USA 90：5873-5877中的修改。对于序列比较可以利用这些数学算法的电脑实现方式以确定序列的同源性，此类实现方式包括但不限于：PC/Gene程序中的CLUSTAL(从Intelligenetics，MountainView，California可获得)；ALLGN程序(版本2.0)以及GCG Wisconsin Genetics SoftwarePack age版本10中的GAP、BESTFIT、BLAST、FASTA以及TFASTA(从Accelr ys Inc.，9685Scranton Road，San Diego，California，USA获得)。
在某些实施例中，编码突变体HPPD多肽或它们的保留了HPPD酶活性的变体的氨基酸可以与任何感兴趣的多核苷酸序列的组合进行叠加从而产生具有一种所希望的性状的植物。术语“性状”是指从特定的序列或序列组得到的表型。例如，编码突变体HPPD多肽或保留了HPPD酶活性的变体的氨基酸的多核苷酸可以与任何其他的编码赋予一种所希望的性状的多肽的多核苷酸进行叠加，所述性状包括但不限于：对于疾病、昆虫以及除草剂的抗性，对于热和干旱的耐受性，缩短作物成熟时间、改进工业加工(例如，用于将淀粉或生物质转化为可发酵的糖类)以及改进的农艺学品质(例如，高油含量和高蛋白含量)。
本领域技术人员所熟知的，两种或更多作用模式的组合在提高受控杂草谱和/或野生型更具耐受性物种或抗性杂草物种上的益处还可扩展到通过人工(转基因或非转基因)在作物中产生除HPPD耐受性作物外的除草剂耐受性的化学品。事实上，可以单独或以多重组合叠加编码以下抗性的性状以提供有效控制或防止杂草演替对除草剂产生抗性能力：草甘膦抗性(如抗性植物或细菌EPSPS、GOX、GAT)、草铵膦抗性(如PAT、Bar)、乙酰乳酸合酶(ALS)抑制性除草剂抗性(如咪唑啉酮、磺酰脲、三唑嘧啶、磺苯胺、嘧啶硫代苯甲酸和其它化学品抗性基因如AHAS、Csrl、SurA等)、苯氧基生长素类除草剂抗性(如芳氧基链烷酸酯双加氧酶-AAD)、麦草畏除草剂抗性(如麦草畏单加氧酶-DMO)、溴草腈抗性(如Bxn)、对八氢番茄红素去饱和酶(PDS)抑制剂的抗性、对光系统II抑制性除草剂的抗性(如psbA)、对光系统I抑制性除草剂的抗性、对原卟啉原氧化酶IX(PPO)抑制性除草剂抗性(如PPO-1)、对苯脲除草剂的抗性(如CYP76B1)、二氯甲氧苯酸降解酶等等。
草甘膦被广泛地使用，因为它控制非常广谱的阔叶和禾本科杂草物种。然而，在草甘膦耐性作物和非作物应用中重复使用草甘膦已经(而且仍将继续)选择使杂草演替为野生型更具有耐性的物种或草甘膦抗性生物型。多数除草剂抗性管理策略建议使用有效用量的罐混除草剂伴侣作为延缓出现抗性杂草的方法，所述除草剂伴侣提供对同一物种的控制，但具有不同的作用模式。将本发明突变的羟基苯丙酮酸双加氧酶多肽的基因与草甘膦耐性性状(和/或其他除草剂耐性性状)叠加可通过允许对同一作物选择性使用草甘膦和HPPD抑制剂除草剂(如苯吡唑草酮、甲基磺草酮或异噁唑草酮)而实现对草甘膦耐性作物中草甘膦抗性杂草物种(被一种或多种HPPD抑制剂除草剂控制的阔叶杂草物种)的控制。这些除草剂的应用可以是在含有不同作用模式的两种或更多除草剂的罐混合物中同时使用、在连续使用(如种植前、出苗前或出苗后)中单个除草剂组合物的单独使用(使用的间隔时间范围从2小时到3个月)，或者备选地，可以在任何时间(从种植作物7个月内到收获作物时(或对于单个除草剂为收获前间隔，取最短者))使用代表可应用每种化合类别的任意数目除草剂的组合。
在控制阔叶杂草中具有灵活性是很重要的，即使用时间、单个除草剂用量和控制顽固或抗性杂草的能力。作物中与草甘膦抗性基因/突变的HPPD基因叠加的草甘膦应用范围可以从250至2500g ae/ha；HPPD抑制剂除草剂(一种或多种)可按照从25-500g ai/ha。这些应用的时间的最佳组合取决于具体的条件、物种和环境。
除草剂制剂(如酯、酸或盐配方或可溶浓缩剂、乳化浓缩剂或可溶液体)和罐混添加剂(如佐剂或相容剂)可显著影响给定的除草剂或一种或多种除草剂的组合的杂草控制。任意前述除草剂的任意化学组合均在本发明的范围内。
此外，可以将编码本发明突变的羟基苯丙酮酸双加氧酶多肽的基因单独或与其它除草剂耐受作物特征叠加后再与一种或多种其它输入(如昆虫抗性、真菌抗性或胁迫耐受性等)或输出(如提高的产量、改进的油量、提高的纤维品质等)性状叠加。因此，本发明可用于提供以灵活且经济地控制任何数目的农学害虫的能力和提高作物品质的完整农学解决方案。
这些叠加的组合可以通过任何方法来产生，这些方法包括但不限于：通过常规的或顶交方法的杂交育种植物或遗传转化。如果这些序列是通过遗传转化这些植物来进行叠加的，所感兴趣的多核苷酸序列可以在任何时间并且以任何次序进行组合。例如，包括一个或多个所希望的性状的转基因植物可以用做通过后续转化而引入另外性状的靶标。这些性状可以在一个共转化方案中与由表达盒的任何组合提供的感兴趣的多核苷酸同时引入。例如，如果将引入两个序列，这两个序列可以包含在分开的表达盒(反式)中或包含在相同的表达盒(顺式)中。这些序列的表达可以通过相同的启动子或通过不同的启动子来驱动。在某些情况下，可能希望的是引入一个抑制所感兴趣的多核苷酸的表达的表达盒。这可以与其他的抑制表达盒或过量表达盒的任何组合进行组合以在该植物中产生所希望的性状组合。进一步认识到的是，多核苷酸序列可以在一个所希望的基因组位置处使用位点特异性重组系统进行叠加。
本发明编码所述突变的羟基苯丙酮酸双加氧酶多肽的基因对HPPD抑制剂除草剂具有较高的耐受性，是重要的除草剂耐受作物和选择标记物特征可能性的基础。
本发明中术语“表达盒”是指能够在适当的宿主细胞中指引一种特定核苷酸序列表达的一种核酸分子，包括有效连接到感兴趣的核苷酸序列(即，一种单独地或与一种或多种编码赋予所希望的性状的多肽的额外的核酸分子相组合而编码一种突变体HPPD多肽或保留了HPPD酶活性的变体的多核苷酸)的启动子，该感兴趣的核苷酸序列有效连接到终止信号。该编码区通常对一种感兴趣的蛋白质进行编码，但是还可以对一种感兴趣的功能性RNA进行编码，例如在正义或反义方向上的反义RNA或一种非翻译RNA。包含该感兴趣的核苷酸序列的表达盒可以是嵌合的，意味着至少一个它的组分相对于至少一个它的其他组分是异源的。该表达盒还可以是一种野生型发生的表达盒，但一定是以在对异源表达有用的重组体形式而获得的。然而，典型地，该表达盒对于宿主是异源的，即，该表达盒的特定DNA序列并不野生型发生于该宿主细胞中，并且必须已通过转化事件而被引入新宿主细胞中。在该表达盒中核苷酸序列的表达可以是在组成型启动子或诱导型启动子的控制之下，该启动子只有当该宿主细胞暴露于一些特殊的外界刺激时才引发转录。另外，该启动子对于一种特定的组织或器官或发育阶段也是特异性的。
本发明涵盖了用能够表达一种感兴趣的多核苷酸(即一种单独地或与一种或多种编码赋予所希望的性状的多肽的额外的核酸分子相组合而编码一种突变体HPPD多肽或它的保留了HPPD酶活性的变体的多核苷酸)的表达盒来转化植物。该表达盒在5’-3’的转录方向上包括转录和翻译的起始区(即启动子)和多核苷酸开放阅读框。该表达盒可以任选地包括在植物中起作用的转录和翻译终止区(即终止区)。在一些实施方案中，该表达盒包括一种选择标记基因从而允许选择稳定的转化体。本发明的表达构建体还可以包括前导序列和/或允许感兴趣的多核苷酸的诱导型表达的序列。
该表达盒的调控序列有效连接到感兴趣的多核苷酸。本发明中所述调控序列包括但不限于启动子、转运肽、终止子、增强子、前导序列、内含子以及其它可操作地连接到编码所述突变的羟基苯丙酮酸双加氧酶多肽的除草剂耐受性基因的调节序列。
所述启动子为植物中可表达的启动子，所述的“植物中可表达的启动子”是指确保与其连接的编码序列在植物细胞内进行表达的启动子。植物中可表达的启动子可为组成型启动子。指导植物内组成型表达的启动子的示例包括但不限于，来源于花椰菜花叶病毒的35S启动子、玉米Ubi启动子、水稻GOS2基因的启动子等。备选地，植物中可表达的启动子可为组织特异的启动子，即该启动子在植物的一些组织内如在绿色组织中指导编码序列的表达水平高于植物的其他组织(可通过常规RNA试验进行测定)，如PEP羧化酶启动子。备选地，植物中可表达的启动子可为创伤诱导启动子。创伤诱导启动子或指导创伤诱导的表达模式的启动子是指当植物经受机械或由昆虫啃食引起的创伤时，启动子调控下的编码序列的表达较正常生长条件下有显著提高。创伤诱导启动子的示例包括但不限于，马铃薯和西红柿的蛋白酶抑制基因(pin I和pin II)和玉米蛋白酶抑制基因(MPI)的启动子。
所述转运肽(又称分泌信号序列或导向序列)是指导转基因产物到特定的细胞器或细胞区室，对受体蛋白质来说，所述转运肽可以是异源的，例如，利用编码叶绿体转运肽序列靶向叶绿体，或者利用‘KDEL'保留序列靶向内质网，或者利用大麦植物凝集素基因的CTPP靶向液泡。
所述前导序列包含但不限于，小RNA病毒前导序列，如EMCV前导序列(脑心肌炎病毒5’非编码区)；马铃薯Y病毒组前导序列，如MDMV(玉米矮缩花叶病毒)前导序列；人类免疫球蛋白质重链结合蛋白质(BiP)；苜蓿花叶病毒的外壳蛋白质mRNA的不翻译前导序列(AMVRNA4)；烟草花叶病毒(TMV)前导序列。
所述增强子包含但不限于，花椰菜花叶病毒(CaMV)增强子、玄参花叶病毒(FMV)增强子、康乃馨风化环病毒(CERV)增强子、木薯脉花叶病毒(CsVMV)增强子、紫茉莉花叶病毒(MMV)增强子、夜香树黄化曲叶病毒(CmYLCV)增强子、木尔坦棉花曲叶病毒(CLCuMV)、鸭跖草黄斑驳病毒(CoYMV)和花生褪绿线条花叶病毒(PCLSV)增强子。
对于单子叶植物应用而言，所述内含子包含但不限于，玉米hsp70内含子、玉米泛素内含子、Adh内含子1、蔗糖合酶内含子或水稻Act1内含子。对于双子叶植物应用而言，所述内含子包含但不限于，CAT-1内含子、pKANNIBAL内含子、PIV2内含子和“超级泛素”内含子。
所述终止子可以为在植物中起作用的适合多聚腺苷酸化信号序列，包括但不限于，来源于农杆菌(Agrobacterium tumefaciens)胭脂碱合成酶(NOS)基因的多聚腺苷酸化信号序列、来源于蛋白酶抑制剂II(pin II)基因的多聚腺苷酸化信号序列、来源于豌豆ssRUBISCO E9基因的多聚腺苷酸化信号序列和来源于α-微管蛋白(α-tubulin)基因的多聚腺苷酸化信号序列。
本发明中所述“有效连接”表示核酸序列的联结，所述联结使得一条序列可提供对相连序列来说需要的功能。在本发明中所述“有效连接”可以为将启动子与感兴趣的序列相连，使得该感兴趣的序列的转录受到该启动子控制和调控。当感兴趣的序列编码蛋白并且想要获得该蛋白的表达时“有效连接”表示：启动子与所述序列相连，相连的方式使得得到的转录物高效翻译。如果启动子与编码序列的连接是转录物融合并且想要实现编码的蛋白的表达时，制造这样的连接，使得得到的转录物中第一翻译起始密码子是编码序列的起始密码子。备选地，如果启动子与编码序列的连接是翻译融合并且想要实现编码的蛋白的表达时，制造这样的连接，使得5’非翻译序列中含有的第一翻译起始密码子与启动子相连结，并且连接方式使得得到的翻译产物与编码想要的蛋白的翻译开放读码框的关系是符合读码框的。可以“有效连接”的核酸序列包括但不限于：提供基因表达功能的序列(即基因表达元件，例如启动子、5’非翻译区域、内含子、蛋白编码区域、3’非翻译区域、聚腺苷化位点和/或转录终止子)、提供DNA转移和/或整合功能的序列(即T-DNA边界序列、位点特异性重组酶识别位点、整合酶识别位点)、提供选择性功能的序列(即抗生素抗性标记物、生物合成基因)、提供可计分标记物功能的序列、体外或体内协助序列操作的序列(即多接头序列、位点特异性重组序列)和提供复制功能的序列(即细菌的复制起点、自主复制序列、着丝粒序列)。
本发明中所述的植物、植物组织或植物细胞的基因组，是指植物、植物组织或植物细胞内的任何遗传物质，且包括细胞核和质体和线粒体基因组。
在本发明中，术语“植物部分”或“植物组织”包括植物细胞、植物原生质体、植物可以由之再生的植物细胞组织培养物、植物愈伤组织、植物簇以及在植物或以下植物的部分中完整的植物细胞，这些植物的部分是如胚、花粉、胚珠、种子、叶、花、枝、果实、核、穗、穗轴、外壳、茎、根、根尖、花药等等。
本发明所述突变的羟基苯丙酮酸双加氧酶多肽可应用于多种植物中，所述双子叶植物包括但不限于苜蓿、菜豆、花椰菜、甘蓝、胡萝卜、芹菜、棉花、黄瓜、茄子、莴苣、甜瓜、豌豆、胡椒、西葫芦、萝卜、油菜、菠菜、大豆、南瓜、番茄、拟南芥、花生或西瓜；优选地，所述双子叶植物是指黄瓜、大豆、拟南芥、烟草、棉花、花生或油菜。所述单子叶植物包括但不限于水稻、高粱、小麦、大麦、黑麦、粟、甘蔗、燕麦或草坪草；优选地，所述单子叶植物是指水稻、高粱、小麦、大麦、粟、甘蔗或燕麦。
在本发明中术语“植物转化”是指将一种抗或耐受除草剂突变体HPPD多核苷酸单独地或与编码赋予所希望的性状的多肽的一种或多种额外的核酸分子结合而克隆到一个表达系统中，它就是被转化到了一种植物细胞中。本发明的受体和目标表达盒可以以多种公知的方法被引入到植物细胞中。在多核苷酸的背景下，术语“引入”(例如，感兴趣的核苷酸构建体)旨在表示以这样一种方式将多核苷酸提供给该植物，使得该多核苷酸获得对一种植物细胞的内部的接近或实现。其中有待引入一个以上的多核苷酸，这些多核苷酸可以作为单个核苷酸构建体的部分而进行组装，或者作为分开的核苷酸构建体，并且可以位于相同的或不同的转化载体上。因此，或作为育种方案的一部分，例如在植物中的在一个单一的转化事件中、在分开的转化事件中可以将这些多核苷酸引入到感兴趣的宿主细胞中。本发明的这些方法并不取决于一种用于引入一个或多个多核苷酸到植物中的具体方法，仅仅是获得这个或这些多核苷酸对于植物的至少一个细胞的内部的接近或实现。在本领域中已知的用于将一个或多个多核苷酸引入到植物中的方法包括但不限于瞬时转化方法、稳定转化方法、病毒介导的方法或基因组编辑技术。
术语“稳定转化”是指将外源基因导入植物基因组，且稳定地整合进该植物及其任何连续世代的基因组中，导致外源基因稳定遗传。
术语“瞬时转化”是指核酸分子或蛋白质导入植物细胞中，执行功能但不整合进植物基因组中，导致外源基因不能稳定遗传。
术语“基因组编辑技术”是指能够对基因组序列进行精确操作，实现基因定点突变、插入、删除等操作的基因组修饰技术。目前基因组编辑技术主要有HE(homingendonuclease，归巢核酸内切酶)、ZFN技术(Zinc-finger nuclease，锌指核酸酶)、TALEN技术(transcription activator-like effector nuclease，转录激活样效应因子核酸酶)、CRISPR技术(Clustered regulatory interspaced short palindromic repeat，成簇规律间隔短回文重复)。
对本领域技术人员而言，用于植物转化的可获得的众多转化载体是已知的，并且与本发明有关的基因可以与任何上述载体结合使用。载体的选择将取决于优选的转化技术以及用于转化的目标种类。对于某些目标种类，可以优选不同的抗生素或除草剂选择标记。在转化中常规使用的选择标记包括赋予对卡那霉素以及相关抗生素或相关除草剂的抗性的nptll基因(此基因被Bevan等人于1983年发表在《自然科学》第304卷184-187页)、赋予对除草剂草丁膦(还被称作草铵膦；参见White等人于1990年发表于《Nucl.AcidsRes》第18卷1062页、Spencer等人于1990年发表于《Theor.Appl.Genet》第79卷625-631页以及美国专利5561236和5276268)抗性的pat和bar基因，赋予对抗生素潮霉素的抗性的hpn基因(Blochinger&Diggelmann，Mol.Cell Biol.4：2929-2931)以及赋予对甲氨蝶呤的抗性的dnfr基因(Bourouis等人1983年于《EMBO J.》第2卷1099-1104页)、赋予对草甘膦的抗性的EPSPS基因(美国专利4940935和5188642)、也赋予对草甘膦的抗性的草甘膦N-乙酰基转移酶(GAT)基因(Castle等人于2004年在《Science》第304卷1151-1154页；美国申请公开专利20070004912，20050246798和20050060767中有描述)以及提供代谢甘露糖的6-磷酸甘露糖异构酶基因(美国专利5767378和5994629有描述)。
用于再生植物的方法在本领域也是熟知的。例如，已经利用Ti质粒载体用于传送外源DNA，以及直接DNA摄入、脂质体、电穿孔、显微注射以及微弹。
本发明中所述种植系统包括对一种或多种除草剂具有耐受性的转基因植物、和/或在所述植物发育的不同阶段可用的除草剂处理的组合，当施用该除草剂时，可有效控制杂草生长且产生高产和/或减弱损伤的植物。
本发明中，所述杂草是指在植物生长环境中与耕种的目的植物竞争的植物。
本发明术语“控制”和/或“防治”是指至少将有效剂量的HPPD抑制剂除草剂直接施用(例如通过喷雾)到植物生长环境中，使杂草发育最小化和/或停止生长。同时，耕种的目的植物在形态上应是正常的，且可在常规方法下培养以用于产物的消耗和/或生成；优选地，与非转基因的野生型植株相比具有减弱的植物损伤和/或具有增加的植物产量。所述具有减弱的植物损伤，具体表现包括但不限于改善的茎秆抗性、和/或提高的籽粒重量等。所述突变的羟基苯丙酮酸双加氧酶多肽对杂草的“控制”和/或“防治”作用是可以独立存在的，不因其它可“控制”和/或“防治”杂草的物质的存在而减弱和/或消失。具体地，转基因植物(含有编码突变的羟基苯丙酮酸双加氧酶多肽的基因)的任何组织同时和/或不同步地，存在和/或产生，所述突变的羟基苯丙酮酸双加氧酶多肽和/或可控制杂草的另一种物质，则所述另一种物质的存在既不影响所述突变的羟基苯丙酮酸双加氧酶多肽对杂草的“控制”和/或“防治”作用，也不能导致所述“控制”和/或“防治”作用完全和/或部分由所述另一种物质实现，而与所述突变的羟基苯丙酮酸双加氧酶多肽无关。
本发明中所述的“植物繁殖体”包括但不限于植物有性繁殖体和植物无性繁殖体。所述植物有性繁殖体包括但不限于植物种子；所述植物无性繁殖体是指植物体的营养器官或某种特殊组织，其可以在离体条件下产生新植株；所述营养器官或某种特殊组织包括但不限于根、茎和叶，例如：以根为无性繁殖体的植物包括草莓和甘薯等；以茎为无性繁殖体的植物包括甘蔗和马铃薯(块茎)等；以叶为无性繁殖体的植物包括芦荟和秋海棠等。
本发明可赋予植物新除草剂抗性性状，并且未观察到对表型包括产量的不良影响。本发明中植物能耐受住如至少一种受试除草剂0.5×、1×、2×、3×、4×或8×一般应用水平。这些耐性水平的提高在本发明的范围之内。例如可对本领域已知的多种技术进行可预见到的优化和进一步发展，以增加给定基因的表达。
本发明提供了一种突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途，具有以下优点：
1、本发明首次公开了对不同物种来源的羟基苯丙酮酸双加氧酶多肽的372位点+383位点进行组合突变可以赋予植物对吡唑啉酮类、异噁唑类和三酮类HPPD抑制剂类除草剂表现出协同增效的耐受性，特别是可以赋予转基因大豆植株耐受4倍大田浓度的苯吡唑草酮、异噁唑草酮和甲基磺草酮，因此在植物上应用前景广阔。
2、本发明羟基苯丙酮酸双加氧酶多肽中的372位点+383位点组合突变与其他突变位点进一步组合不影响单独372位点+383位点组合突变对HPPD抑制剂类除草剂表现出协同增效的耐受性，证明了HPPD多肽的372位点+383位点组合突变赋予植物对HPPD抑制剂类除草剂耐受性的重要性和稳定性。
3、在本发明羟基苯丙酮酸双加氧酶多肽的372位点+383位点组合突变的基础上，优化HPPD氨基酸序列的C端对提高植物对异噁唑草酮的耐受性将是有益的。
下面通过附图和实施例，对本发明的技术方案做进一步的详细描述。
附图说明
图1为本发明含有所述AsHPPDm-F372A-F383W-02核苷酸序列的拟南芥重组表达载体DBN11726结构示意图；
图2为本发明对照重组表达载体DBN11726N结构示意图；
图3为本发明不同物种来源的HPPD进化树图谱。
具体实施方式
下面通过具体实施例进一步说明本发明突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途的技术方案。
第一实施例、选择AsHPPD的372位点和383位点进行组合突变(F372A+F383W)并验证其突变效果
1、获得AsHPPD和AsHPPDm-F372A-F383W基因
燕麦野生型HPPD(AsHPPD)的氨基酸序列，如序列表中SEQ ID NO：1所示；编码所述AsHPPD的AsHPPD-01核苷酸序列，如序列表中SEQ ID NO：2所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPD的AsHPPD-02核苷酸序列，如序列表中SEQ ID NO：3所示。
将所述AsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得AsHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：4所示，编码所述AsHPPDm-F372A氨基酸序列的AsHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：5所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F372A氨基酸序列的AsHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：6所示。
将所述AsHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AsHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：7所示，编码所述AsHPPDm-F383W氨基酸序列的AsHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：8所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F383W氨基酸序列的AsHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：9所示。
将所述AsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AsHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：10所示，编码所述AsHPPDm-F372A-F383W氨基酸序列的AsHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：11所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F372A-F383W氨基酸序列的AsHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：12所示。
2、合成上述核苷酸序列
合成的所述AsHPPD-02核苷酸序列(SEQ ID NO：3)、AsHPPDm-F372A-02核苷酸序列(SEQ ID NO：6)、AsHPPDm-F383W-02核苷酸序列(SEQ ID NO：9)和AsHPPDm-F372A-F383W-02核苷酸序列(SEQ ID NO：12)的5’和3’端分别连接通用接头引物1：
5’端通用接头引物1：5’-agtttttctgattaacagactagt-3’，如序列表中SEQ ID NO：230所示；
3’端通用接头引物1：5’-caaatgtttgaacgatcggcgcgcc-3’，如序列表中SEQ IDNO：231所示。
3、构建含有燕麦HPPD基因(F372A-F383W)的拟南芥重组表达载体
利用限制性内切酶Spe I和Asc I对植物表达载体DBNBC-01进行双酶切反应，从而对植物表达载体线性化，酶切产物纯化得到线性化的DBNBC-01表达载体骨架(载体骨架：pCAMBIA2301(CAMBIA机构可以提供))，将连接所述通用接头引物1的AsHPPDm-F372A-F383W-02核苷酸序列与所述线性化的DBNBC-01表达载体骨架进行重组反应，操作步骤按照Takara公司In-Fusion无缝连接产品试剂盒(Clontech，CA，USA，CAT：121416)说明书进行，构建成重组表达载体DBN11726，其结构示意图如图1所示(Spec：壮观霉素基因；RB：右边界；eFMV：玄参花叶病毒的34S增强子(SEQ ID NO：13)；prBrCBP：油菜真核延长因子基因1α(Tsfl)的启动子(SEQ ID NO：14)；spAtCTP2：拟南芥叶绿体转运肽(SEQ ID NO：15)；EPSPS：5-烯醇丙酮酸莽草酸-3-磷酸合酶基因(SEQ ID NO：16)；tPsE9：豌豆RbcS基因的终止子(SEQ ID NO：17)；prAtUbi10：拟南芥泛素(Ubiquitin)10基因的启动子(SEQ ID NO：18)；AsHPPDm-F372A-F383W-02：AsHPPDm-F372A-F383W-02核苷酸序列(SEQ ID NO：12)；tNos：胭脂碱合成酶基因的终止子(SEQ ID NO：19)；pr35S-01：花椰菜花叶病毒35S启动子(SEQ IDNO：20)；PAT：膦丝菌素N-乙酰基转移酶基因(SEQ ID NO：21)；t35S：花椰菜花叶病毒35S终止子(SEQ ID NO：22)；LB：左边界)。
将重组表达载体DBN11726用热激方法转化大肠杆菌T1感受态细胞，其热激条件为：50μL大肠杆菌T1感受态细胞、10μL质粒DNA(重组表达载体DBN11726)，42℃水浴30s；37℃振荡培养1h(100rpm转速下摇床摇动)；然后在含50mg/L壮观霉素(Spectinomycin)的所述LB固体平板上于温度37℃条件下培养12h，挑取白色菌落，在LB液体培养基(胰蛋白胨10g/L、酵母提取物5g/L、NaCl10g/L、壮观霉素50mg/L，用NaOH调pH至7.5)中于温度37℃条件下培养过夜。碱法提取其质粒：将菌液在12000rpm转速下离心1min，去上清液，沉淀菌体用100μl冰预冷的溶液I(25mM Tris-HCl、10mM EDTA(乙二胺四乙酸)、50mM葡萄糖，pH8.0)悬浮；加入200μL新配制的溶液II(0.2M NaOH、1％SDS(十二烷基硫酸钠))，将管子颠倒4次，混合，置冰上3-5min；加入150μL冰冷的溶液III(3M醋酸钾、5M醋酸)，立即充分混匀，冰上放置5-10min；于温度4℃、转速12000rpm条件下离心5min，在上清液中加入2倍体积无水乙醇，混匀后室温放置5min；于温度4℃、转速12000rpm条件下离心5min，弃上清液，沉淀用浓度(V/V)为70％的乙醇洗涤后晾干；加入30μL含RNase(20μg/mL)的TE(10mM Tris-HCl、1mMEDTA，pH8.0)溶解沉淀；于温度37℃下水浴30min，消化RNA；于温度-20℃保存备用。将提取的质粒进行测序鉴定，结果表明重组表达载体DBN11726在Spe I和Asc I位点间的核苷酸序列为序列表中SEQ ID NO：12所示的核苷酸序列，即所述AsHPPDm-F372A-F383W-02核苷酸序列。
按照上述构建重组表达载体DBN11726的方法，将连接所述通用接头引物1的所述AsHPPD-02核苷酸序列、AsHPPDm-F372A-02核苷酸序列和AsHPPDm-F383W-02核苷酸序列分别与所述线性化的DBNBC-01表达载体骨架进行重组反应，依次得到重组表达载体DBN11727、DBN11728和DBN11729。分别测序验证重组表达载体DBN11727、DBN11728和DBN11729中的核苷酸序列为分别含有序列表中SEQ ID NO：3所示核苷酸序列、序列表中SEQID NO：6所示核苷酸序列和序列表中SEQ ID NO：9所示核苷酸序列，即所述AsHPPD-02核苷酸序列、AsHPPDm-F372A-02核苷酸序列和AsHPPDm-F383W-02核苷酸序列分别正确插入。
构建对照重组表达载体DBN11726N，其载体结构如图2所示(Spec：壮观霉素基因；RB：右边界；eFMV：玄参花叶病毒的34S增强子(SEQ ID NO：13)；prBrCBP：油菜真核延长因子基因1α(Tsfl)的启动子(SEQ ID NO：14)；spAtCTP2：拟南芥叶绿体转运肽(SEQ ID NO：15)；EPSPS：5-烯醇丙酮酸莽草酸-3-磷酸合酶基因(SEQ ID NO：16)；tPsE9：豌豆RbcS基因的终止子(SEQ ID NO：17)；pr35S-01：花椰菜花叶病毒35S启动子(SEQ ID NO：20)；PAT：膦丝菌素N-乙酰基转移酶基因(SEQ ID NO：21)；t35S：花椰菜花叶病毒35S终止子(SEQ ID NO：22)；LB：左边界)。
4、拟南芥重组表达载体转化农杆菌
对己经构建正确的重组表达载体DBN11726、DBN11727、DBN11728、DBN11729和DBN11726N分别用液氮法转化到农杆菌GV3101中，其转化条件为：100μL农杆菌GV3101、3μL质粒DNA(重组表达载体)；置于液氮中10min，37℃温水浴10min；将转化后的农杆菌GV3101接种于LB试管中于温度28℃、转速为200rpm条件下培养2h，涂于含50mg/L的利福平(Rifampicin)和50mg/L的壮观霉素的所述LB固体平板上直至长出阳性单克隆，挑取单克隆培养并提取其质粒，将提取的质粒进行测序鉴定，结果表明重组表达载体DBN11726至DBN11729以及DBN11726N结构完全正确。
5、转基因拟南芥植株的获得
将野生型拟南芥种子悬浮于0.1％(w/v)琼脂糖溶液中。将悬浮的种子在4℃下保存2天以完成对休眠的需要以保证种子同步萌发。用蛭石混合马粪土并用水底部灌溉至湿润，使土壤混合物排水24h。将预处理后的种子种在土壤混合物上并用保湿罩覆盖7天。使种子萌发并在恒温(22℃)恒湿(40-50％)光强度为120-150μmol/m2s-1的长日照条件(16h光照/8h黑暗)下在温室中培养植物。开始用霍格兰营养液灌溉植物，接着用去离子水灌溉，保持土壤潮湿但不湿透。
使用花浸泡法转化拟南芥。用选取的农杆菌菌落接种一份或多份15-30mL含壮观霉素(50mg/L)和利福平(10mg/L)的LB培养液(胰蛋白胨10g/L、酵母提取物5g/L、NaCl 10g/L，用NaOH调pH至7.5)的预培养物。以220rpm转速将预培养物在温度28℃恒速摇动孵育过夜。每个预培养物用于接种两份500ml含壮观霉素(50mg/L)和利福平(10mg/L)的所述LB培养液的培养物并将培养物在温度28℃持续摇动孵育过夜。室温以转速约4000rpm离心20min沉淀细胞，弃去得到的上清液。将细胞沉淀轻柔重悬于500mL渗透培养基中，所述渗透培养基含有1/2×MS盐/B5维生素、10％(w/v)蔗糖、0.044μM苄氨基嘌呤(10μL/L(1mg/mL DMSO中的原液))和300μL/L Silvet L-77。将约1月龄的拟南芥植物在含重悬细胞的渗透培养基中浸泡5min，确保浸没最新的花序。接着将拟南芥植物侧面放倒并覆盖，黑暗环境下保湿24h，在温度22℃以16h光照/8h黑暗的光周期正常培养拟南芥植物。约4周后收获种子。
将新收获的(AsHPPD-02核苷酸序列、AsHPPDm-F372A-02核苷酸序列、AsHPPDm-F383W-02核苷酸序列、AsHPPDm-F372A-F383W-02核苷酸序列和对照重组表达载体DBN11726N)T1种子在室温干燥7天。将种子种在26.5cm×51cm萌发盘中，每盘接受200mg T1种子(约10000个种子)，所述种子事先已悬浮于蒸馏水中并在温度4℃下保存2天以完成对休眠的需要以保证种子同步萌发。
用蛭石混合马粪土并用水底部灌溉至湿润，利用重力排水。用移液管将预处理后的种子均匀地种在土壤混合物上，并用保湿罩覆盖4-5天。在使用出苗后喷洒草铵膦(选择共转化的PAT基因)进行最初转化体选择的前1天移去罩。
在7个种植天数后(DAP)并于11DAP再次使用DeVilbiss压缩空气喷嘴以10mL/盘(703L/ha)的喷洒体积用Liberty除草剂(200g ai/L的草铵膦)的0.2％溶液喷洒T1植物(分别为子叶期和2-4叶期)，以提供每次应用280gai/ha有效量的草铵膦。在最后喷洒后4-7天鉴定存活株(生长活跃的植物)，并分别移植到用马粪土和蛭石制备的7cm×7cm的方盆中(每盘3-5棵)。用保湿罩覆盖移植的植物3-4天，并如前置于温度22℃培养室中或直接移入温室。接着移去罩并在测试突变的HPPD基因提供HPPD抑制剂除草剂耐受性的能力之前至少1天将植物栽种到温室(温度22±5℃，50±30％RH，14h光照：10h黑暗，最小500μE/m2s-1野生型+补充光)。
6、含有AsHPPDm-F372A-F383W-02核苷酸序列的转基因拟南芥植株的除草剂耐受性效果检测
首先使用草铵膦选择方案从未转化种子背景中选择T1转化体。分别将转入AsHPPD-02核苷酸序列的拟南芥T1植株(AsHPPD-02)、转入AsHPPDm-F372A-02核苷酸序列的拟南芥T1植株(AsHPPDm-F372A-02)、转入AsHPPDm-F383W-02核苷酸序列的拟南芥T1植株(AsHPPDm-F383W-02)、转入AsHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(AsHPPDm-F372A-F383W-02)、转入对照重组表达载体DBN11726N的拟南芥T1植株(DBN11726N)和野生型拟南芥植株(CK)(播种后18天)分别用3种浓度的苯吡唑草酮(100gai/ha(4倍大田浓度，4×)、200g ai/ha(8倍大田浓度，8×)和0g ai/ha(水，0×))、3种浓度的异噁唑草酮(140g ai/ha(2倍大田浓度，2×)、280g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))和3种浓度的甲基磺草酮(210g ai/ha(2倍大田浓度，2×)、420g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))进行喷洒以检测拟南芥的除草剂耐受性。在喷施7天(7DAT)后，根据叶片白化面积比例(叶片白化面积比例＝叶片白化面积/叶片总面积×100％)来统计每株植株受除草剂的损伤程度：以基本无白化表型为0级，叶片白化面积比例小于50％为1级，叶片白化面积比例大于50％为2级，叶片白化面积比例100％为3级。
按照公式X＝[∑(N×S)/(T×M)]×100对每个重组表达载体的转化事件抗性表现进行评分(X-药害评分、N-同级受害株数、S-药害等级数、T-总株数、M-最高药害等级)，根据分值进行抗性评价：高抗植株(0-15分)、中抗植株(16-33分)、低抗植株(34-67分)、不抗植株(68-100分)。实验结果如表1所示。
表1、转基因拟南芥T1植株对苯吡唑草酮的耐受性实验结果
表1的结果表明：相比于CK，对于4倍或8倍大田浓度的苯吡唑草酮，AsHPPDm-F372A-02、AsHPPDm-F383W-02和AsHPPDm-F372A-F383W-02全部表现出高抗的耐受性，而AsHPPD-02和DBN11726N对苯吡唑草酮则不具有耐受性。
表2、转基因拟南芥T1植株对异噁唑草酮的耐受性实验结果
表2的结果表明：(1)相比于CK，AsHPPDm-F372A-02、AsHPPDm-F383W-02和AsHPPDm-F372A-F383W-02对不同浓度的异噁唑草酮产生了不同程度的耐受性，而AsHPPD-02和DBN11726N对异噁唑草酮不具有耐受性；(2)对于2倍大田浓度的异噁唑草酮，AsHPPDm-F372A-02、AsHPPDm-F383W-02和AsHPPDm-F372A-F383W-02分别表现出中抗、中抗和高抗的耐受性，由此说明，对野生型HPPD氨基酸序列的372位点和383位点进行组合突变(F372A+F383W)的效果优于F372A或F383W单位点突变的效果；(3)对于4倍大田浓度的异噁唑草酮，AsHPPDm-F372A-02、AsHPPDm-F383W-02和AsHPPDm-F372A-F383W-02分别表现出低抗、低抗和高抗的耐受性，由此说明，对野生型HPPD氨基酸序列的372位点和383位点进行组合突变(F372A+F383W)的效果不仅优于F372A或F383W单位点突变的效果，且进一步表现出协同增效的除草剂耐受性效果。
表3、转基因拟南芥T1植株对甲基磺草酮的耐受性实验结果
表3的结果表明：(1)相比于CK，AsHPPDm-F372A-02、AsHPPDm-F383W-02和AsHPPDm-F372A-F383W-02对不同浓度的甲基磺草酮产生了不同程度的耐受性，而AsHPPD-02和DBN11726N对甲基磺草酮不具有耐受性；(2)对于2倍大田浓度的甲基磺草酮，AsHPPDm-F372A-02、AsHPPDm-F383W-02和AsHPPDm-F372A-F383W-02分别表现出中抗、中抗和高抗的耐受性，由此说明，对野生型HPPD氨基酸序列的372位点和383位点进行组合突变(F372A+F383W)的效果优于F372A或F383W单位点突变的效果；(3)对于4倍大田浓度的甲基磺草酮，AsHPPDm-F372A-02、AsHPPDm-F383W-02和AsHPPDm-F372A-F383W-02分别表现出低抗、低抗和高抗的耐受性，由此说明，对野生型HPPD氨基酸序列的372位点和383位点进行组合突变(F372A+F383W)的效果不仅优于F372A或F383W单位点突变的效果，且进一步表现出协同增效的除草剂耐受性效果。
上述表2至表3充分说明野生型HPPD氨基酸序列的372位点和383位点进行组合突变(F372A+F383W)具有协同增效的HPPD抑制剂除草剂耐受性效果。
第二实施例、对不同物种来源的HPPD氨基酸序列的372位点和383位点进行组合突变(F372A+F383W)并验证其突变效果
为了进一步验证HPPD氨基酸序列的372位点和383位点组合突变的协同效果，对不同物种来源的HPPD进化树(如图3所示)进行了分析，选取不同分支上有代表性物种来源的HPPD，对其氨基酸序列的372位点和383位点进行组合突变(F372A+F383W)以验证其突变效果。
1、获得不同物种来源的HPPD和突变的HPPD(F372A+F383W)
(1)获取拟南芥突变的HPPD(F372A+F383W)
拟南芥野生型HPPD(AtHPPD)的氨基酸序列，如序列表中SEQ ID NO：23所示；编码所述AtHPPD的AtHPPD-01核苷酸序列，如序列表中SEQ ID NO：24所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AtHPPD的AtHPPD-02核苷酸序列，如序列表中SEQ ID NO：25所示。
将所述AtHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得AtHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：26所示，编码所述AtHPPDm-F372A氨基酸序列的AtHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：27所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AtHPPDm-F372A氨基酸序列的AtHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：28所示。
将所述AtHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AtHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：29所示，编码所述AtHPPDm-F383W氨基酸序列的AtHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：30所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AtHPPDm-F383W氨基酸序列的AtHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：31所示。
将所述AtHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AtHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：32所示，编码所述AtHPPDm-F372A-F383W氨基酸序列的AtHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：33所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AtHPPDm-F372A-F383W氨基酸序列的AtHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：34所示。
(2)获取苜蓿突变的HPPD(F372A+F383W)
苜蓿野生型HPPD(MsHPPD)的氨基酸序列，如序列表中SEQ ID NO：35所示；编码所述MsHPPD的MsHPPD-01核苷酸序列，如序列表中SEQ ID NO：36所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述MsHPPD的MsHPPD-02核苷酸序列，如序列表中SEQ ID NO：37所示。
将所述MsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得MsHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：38所示，编码所述MsHPPDm-F372A氨基酸序列的MsHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：39所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述MsHPPDm-F372A氨基酸序列的MsHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：40所示。
将所述MsHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得MsHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：41所示，编码所述MsHPPDm-F383W氨基酸序列的MsHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：42所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述MsHPPDm-F383W氨基酸序列的MsHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：43所示。
将所述MsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得MsHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：44所示，编码所述MsHPPDm-F372A-F383W氨基酸序列的MsHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：45所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述MsHPPDm-F372A-F383W氨基酸序列的MsHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：46所示。
(3)获取棉花突变的HPPD(F372A+F383W)
棉花野生型HPPD(GsHPPD)的氨基酸序列，如序列表中SEQ ID NO：47所示；编码所述GsHPPD的GsHPPD-01核苷酸序列，如序列表中SEQ ID NO：48所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述GsHPPD的GsHPPD-02核苷酸序列，如序列表中SEQ ID NO：49所示。
将所述GsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得GsHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：50所示，编码所述GsHPPDm-F372A氨基酸序列的GsHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：51所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述GsHPPDm-F372A氨基酸序列的GsHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：52所示。
将所述GsHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得GsHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：53所示，编码所述GsHPPDm-F383W氨基酸序列的GsHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：54所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述GsHPPDm-F383W氨基酸序列的GsHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：55所示。
将所述GsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得GsHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：56所示，编码所述GsHPPDm-F372A-F383W氨基酸序列的GsHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：57所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述GsHPPDm-F372A-F383W氨基酸序列的GsHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：58所示。
(4)获取油菜突变的HPPD(F372A+F383W)
油菜野生型HPPD(BnHPPD)的氨基酸序列，如序列表中SEQ ID NO：59所示；编码所述BnHPPD的BnHPPD-01核苷酸序列，如序列表中SEQ ID NO：60所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述BnHPPD的BnHPPD-02核苷酸序列，如序列表中SEQ ID NO：61所示。
将所述BnHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得BnHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：62所示，编码所述BnHPPDm-F372A氨基酸序列的BnHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：63所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述BnHPPDm-F372A氨基酸序列的BnHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：64所示。
将所述BnHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得BnHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：65所示，编码所述BnHPPDm-F383W氨基酸序列的BnHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：66所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述BnHPPDm-F383W氨基酸序列的BnHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：67所示。
将所述BnHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得BnHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：68所示，编码所述BnHPPDm-F372A-F383W氨基酸序列的BnHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：69所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述BnHPPDm-F372A-F383W氨基酸序列的BnHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：70所示。
(5)获取大豆突变的HPPD(F372A+F383W)
大豆野生型HPPD(GmHPPD)的氨基酸序列，如序列表中SEQ ID NO：71所示；编码所述GmHPPD的GmHPPD-01核苷酸序列，如序列表中SEQ ID NO：72所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述GmHPPD的GmHPPD-02核苷酸序列，如序列表中SEQ ID NO：73所示。
将所述GmHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得GmHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：74所示，编码所述GmHPPDm-F372A氨基酸序列的GmHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：75所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述GmHPPDm-F372A氨基酸序列的GmHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：76所示。
将所述GmHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得GmHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：77所示，编码所述GmHPPDm-F383W氨基酸序列的GmHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：78所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述GmHPPDm-F383W氨基酸序列的GmHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：79所示。
将所述GmHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得GmHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：80所示，编码所述GmHPPDm-F372A-F383W氨基酸序列的GmHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：81所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述GmHPPDm-F372A-F383W氨基酸序列的GmHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：82所示。
(6)获取烟草突变的HPPD(F372A+F383W)
烟草野生型HPPD(NtHPPD)的氨基酸序列，如序列表中SEQ ID NO：83所示；编码所述NtHPPD的NtHPPD-01核苷酸序列，如序列表中SEQ ID NO：84所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述NtHPPD的NtHPPD-02核苷酸序列，如序列表中SEQ ID NO：85所示。
将所述NtHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得NtHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：86所示，编码所述NtHPPDm-F372A氨基酸序列的NtHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：87所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述NtHPPDm-F372A氨基酸序列的NtHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：88所示。
将所述NtHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得NtHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：89所示，编码所述NtHPPDm-F383W氨基酸序列的NtHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：90所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述NtHPPDm-F383W氨基酸序列的NtHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：91所示。
将所述NtHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得NtHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：92所示，编码所述NtHPPDm-F372A-F383W氨基酸序列的NtHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：93所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述NtHPPDm-F372A-F383W氨基酸序列的NtHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：94所示。
(7)获取水稻突变的HPPD(F372A+F383W)
水稻野生型HPPD(OsHPPD)的氨基酸序列，如序列表中SEQ ID NO：95所示；编码所述OsHPPD的OsHPPD-01核苷酸序列，如序列表中SEQ ID NO：96所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述OsHPPD的OsHPPD-02核苷酸序列，如序列表中SEQ ID NO：97所示。
将所述OsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得OsHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：98所示，编码所述OsHPPDm-F372A氨基酸序列的OsHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：99所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述OsHPPDm-F372A氨基酸序列的OsHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：100所示。
将所述OsHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得OsHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：101所示，编码所述OsHPPDm-F383W氨基酸序列的OsHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：102所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述OsHPPDm-F383W氨基酸序列的OsHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：103所示。
将所述OsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得OsHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：104所示，编码所述OsHPPDm-F372A-F383W氨基酸序列的OsHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：105所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述OsHPPDm-F372A-F383W氨基酸序列的OsHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：106所示。
(8)获取高粱突变的HPPD(F372A+F383W)
高粱野生型HPPD(SbHPPD)的氨基酸序列，如序列表中SEQ ID NO：107所示；编码所述SbHPPD的SbHPPD-01核苷酸序列，如序列表中SEQ ID NO：108所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述SbHPPD的SbHPPD-02核苷酸序列，如序列表中SEQ ID NO：109所示。
将所述SbHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得SbHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：110所示，编码所述SbHPPDm-F372A氨基酸序列的SbHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：111所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述SbHPPDm-F372A氨基酸序列的SbHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：112所示。
将所述SbHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得SbHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：113所示，编码所述SbHPPDm-F383W氨基酸序列的SbHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：114所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述SbHPPDm-F383W氨基酸序列的SbHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：115所示。
将所述SbHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得SbHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：116所示，编码所述SbHPPDm-F372A-F383W氨基酸序列的SbHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：117所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述SbHPPDm-F372A-F383W氨基酸序列的SbHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：118所示。
(9)获取大麦突变的HPPD(F372A+F383W)
大麦野生型HPPD(HvHPPD)的氨基酸序列，如序列表中SEQ ID NO：119所示；编码所述HvHPPD的HvHPPD-01核苷酸序列，如序列表中SEQ ID NO：120所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HvHPPD的HvHPPD-02核苷酸序列，如序列表中SEQ ID NO：121所示。
将所述HvHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得HvHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：122所示，编码所述HvHPPDm-F372A氨基酸序列的HvHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：123所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HvHPPDm-F372A氨基酸序列的HvHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：124所示。
将所述HvHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得HvHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：125所示，编码所述HvHPPDm-F383W氨基酸序列的HvHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：126所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HvHPPDm-F383W氨基酸序列的HvHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：127所示。
将所述HvHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得HvHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：128所示，编码所述HvHPPDm-F372A-F383W氨基酸序列的HvHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：129所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HvHPPDm-F372A-F383W氨基酸序列的HvHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：130所示。
(10)获取玉米突变的HPPD(F372A+F383W)
玉米野生型HPPD(ZmHPPD)的氨基酸序列，如序列表中SEQ ID NO：131所示；编码所述ZmHPPD的ZmHPPD-01核苷酸序列，如序列表中SEQ ID NO：132所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述ZmHPPD的ZmHPPD-02核苷酸序列，如序列表中SEQ ID NO：133所示。
将所述ZmHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得ZmHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：134所示，编码所述ZmHPPDm-F372A氨基酸序列的ZmHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：135所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述ZmHPPDm-F372A氨基酸序列的ZmHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：136所示。
将所述ZmHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得ZmHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：137所示，编码所述ZmHPPDm-F383W氨基酸序列的ZmHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：138所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述ZmHPPDm-F383W氨基酸序列的ZmHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：139所示。
将所述ZmHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得ZmHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：140所示，编码所述ZmHPPDm-F372A-F383W氨基酸序列的ZmHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：141所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述ZmHPPDm-F372A-F383W氨基酸序列的ZmHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：142所示。
(11)获取荧光假单胞菌突变的HPPD(F372A+F383W)
荧光假单胞菌野生型HPPD(PfHPPD)的氨基酸序列，如序列表中SEQ ID NO：143所示；编码所述PfHPPD的PfHPPD-01核苷酸序列，如序列表中SEQ ID NO：144所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述PfHPPD的PfHPPD-02核苷酸序列，如序列表中SEQ ID NO：145所示。
将所述PfHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得PfHPPDm-F372A氨基酸序列，如序列表中SEQ ID NO：146所示，编码所述PfHPPDm-F372A氨基酸序列的PfHPPDm-F372A-01核苷酸序列，如序列表中SEQ ID NO：147所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述PfHPPDm-F372A氨基酸序列的PfHPPDm-F372A-02核苷酸序列，如序列表中SEQ ID NO：148所示。
将所述PfHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得PfHPPDm-F383W氨基酸序列，如序列表中SEQ ID NO：149所示，编码所述PfHPPDm-F383W氨基酸序列的PfHPPDm-F383W-01核苷酸序列，如序列表中SEQ ID NO：150所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述PfHPPDm-F383W氨基酸序列的PfHPPDm-F383W-02核苷酸序列，如序列表中SEQ ID NO：151所示。
将所述PfHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得PfHPPDm-F372A-F383W氨基酸序列，如序列表中SEQ ID NO：152所示，编码所述PfHPPDm-F372A-F383W氨基酸序列的PfHPPDm-F372A-F383W-01核苷酸序列，如序列表中SEQ ID NO：153所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述PfHPPDm-F372A-F383W氨基酸序列的PfHPPDm-F372A-F383W-02核苷酸序列，如序列表中SEQ ID NO：154所示。
2、构建含有不同物种来源突变HPPD(F372A+F383W组合位点突变、F372A单位点突变或F383W单位点突变)的拟南芥重组表达载体
按照上述第一实施例3中构建含有AsHPPDm-F372A-F383W-02核苷酸序列的重组表达载体DBN11726的方法，将连接所述通用接头引物1的所述AtHPPD-02核苷酸序列、AtHPPDm-F372A-02核苷酸序列、AtHPPDm-F383W-02核苷酸序列、AtHPPDm-F372A-F383W-02核苷酸序列、MsHPPD-02核苷酸序列、MsHPPDm-F372A-02核苷酸序列、MsHPPDm-F383W-02核苷酸序列、MsHPPDm-F372A-F383W-02核苷酸序列、GsHPPD-02核苷酸序列、GsHPPDm-F372A-02核苷酸序列、GsHPPDm-F383W-02核苷酸序列、GsHPPDm-F372A-F383W-02核苷酸序列、BnHPPD-02核苷酸序列、BnHPPDm-F372A-02核苷酸序列、BnHPPDm-F383W-02核苷酸序列、BnHPPDm-F372A-F383W-02核苷酸序列、GmHPPD-02核苷酸序列、GmHPPDm-F372A-02核苷酸序列、GmHPPDm-F383W-02核苷酸序列、GmHPPDm-F372A-F383W-02核苷酸序列、NtHPPD-02核苷酸序列、NtHPPDm-F372A-02核苷酸序列、NtHPPDm-F383W-02核苷酸序列、NtHPPDm-F372A-F383W-02核苷酸序列、OsHPPD-02核苷酸序列、OsHPPDm-F372A-02核苷酸序列、OsHPPDm-F383W-02核苷酸序列、OsHPPDm-F372A-F383W-02核苷酸序列、SbHPPD-02核苷酸序列、SbHPPDm-F372A-02核苷酸序列、SbHPPDm-F383W-02核苷酸序列、SbHPPDm-F372A-F383W-02核苷酸序列、HvHPPD-02核苷酸序列、HvHPPDm-F372A-02核苷酸序列、HvHPPDm-F383W-02核苷酸序列、HvHPPDm-F372A-F383W-02核苷酸序列、ZmHPPD-02核苷酸序列、ZmHPPDm-F372A-02核苷酸序列、ZmHPPDm-F383W-02核苷酸序列、ZmHPPDm-F372A-F383W-02核苷酸序列、PfHPPD-02核苷酸序列、PfHPPDm-F372A-02核苷酸序列、PfHPPDm-F383W-02核苷酸序列和PfHPPDm-F372A-F383W-02核苷酸序列分别与所述线性化的DBNBC-01表达载体骨架进行重组反应，依次得到重组表达载体DBN11730至DBN11773。测序验证重组表达载体DBN11730至DBN11773中上述核苷酸序列正确插入。
3、拟南芥重组表达载体转化农杆菌
按照上述第一实施例4中拟南芥重组表达载体转化农杆菌的方法，将己经构建正确的重组表达载体DBN11730至DBN11773以及第一实施例3中构建的对照重组表达载体DBN11726N分别用液氮法转化到农杆菌GV3101中，测序验证结果表明重组表达载体DBN11730至DBN11773以及DBN11726N结构完全正确。
4、转入不同物种来源突变的HPPD(F372A+F383W组合位点突变、F372A单位点突变或F383W单位点突变)的拟南芥植株的除草剂耐受性效果检测
按照上述第一实施例5中的方法将拟南芥花序浸泡在本实施例3的所述农杆菌菌液中，以将本实施例2中构建的重组表达载体DBN11730至DBN11773以及第一实施例3中构建的对照重组表达载体DBN11726N中的T-DNA转入到拟南芥染色体中，获得相应的转基因拟南芥植株，即转入AtHPPD-02核苷酸序列的拟南芥T1植株(AtHPPD-02)、转入AtHPPDm-F372A-02核苷酸序列的拟南芥T1植株(AtHPPDm-F372A-02)、转入AtHPPDm-F383W-02核苷酸序列的拟南芥T1植株(AtHPPDm-F383W-02)、转入AtHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(AtHPPDm-F372A-F383W-02)、转入MsHPPD-02核苷酸序列的拟南芥T1植株(MsHPPD-02)、转入MsHPPDm-F372A-02核苷酸序列的拟南芥T1植株(MsHPPDm-F372A-02)、转入MsHPPDm-F383W-02核苷酸序列的拟南芥T1植株(MsHPPDm-F383W-02)、转入MsHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(MsHPPDm-F372A-F383W-02)、转入GsHPPD-02核苷酸序列的拟南芥T1植株(GsHPPD-02)、转入GsHPPDm-F372A-02核苷酸序列的拟南芥T1植株(GsHPPDm-F372A-02)、转入GsHPPDm-F383W-02核苷酸序列的拟南芥T1植株(GsHPPDm-F383W-02)、转入GsHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(GsHPPDm-F372A-F383W-02)、转入BnHPPD-02核苷酸序列的拟南芥T1植株(BnHPPD-02)、转入BnHPPDm-F372A-02核苷酸序列的拟南芥T1植株(BnHPPDm-F372A-02)、转入BnHPPDm-F383W-02核苷酸序列的拟南芥T1植株(BnHPPDm-F383W-02)、转入BnHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(BnHPPDm-F372A-F383W-02)、转入GmHPPD-02核苷酸序列的拟南芥T1植株(GmHPPD-02)、转入GmHPPDm-F372A-02核苷酸序列的拟南芥T1植株(GmHPPDm-F372A-02)、转入GmHPPDm-F383W-02核苷酸序列的拟南芥T1植株(GmHPPDm-F383W-02)、转入GmHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(GmHPPDm-F372A-F383W-02)、转入NtHPPD-02核苷酸序列的拟南芥T1植株(NtHPPD-02)、转入NtHPPDm-F372A-02核苷酸序列的拟南芥T1植株(NtHPPDm-F372A-02)、转入NtHPPDm-F383W-02核苷酸序列的拟南芥T1植株(NtHPPDm-F383W-02)、转入NtHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(NtHPPDm-F372A-F383W-02)、转入OsHPPD-02核苷酸序列的拟南芥T1植株(OsHPPD-02)、转入OsHPPDm-F372A-02核苷酸序列的拟南芥T1植株(OsHPPDm-F372A-02)、转入OsHPPDm-F383W-02核苷酸序列的拟南芥T1植株(OsHPPDm-F383W-02)、转入OsHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(OsHPPDm-F372A-F383W-02)、转入SbHPPD-02核苷酸序列的拟南芥T1植株(SbHPPD-02)、转入SbHPPDm-F372A-02核苷酸序列的拟南芥T1植株(SbHPPDm-F372A-02)、转入SbHPPDm-F383W-02核苷酸序列的拟南芥T1植株(SbHPPDm-F383W-02)、转入SbHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(SbHPPDm-F372A-F383W-02)、转入HvHPPD-02核苷酸序列的拟南芥T1植株(HvHPPD-02)、转入HvHPPDm-F372A-02核苷酸序列的拟南芥T1植株(HvHPPDm-F372A-02)、转入HvHPPDm-F383W-02核苷酸序列的拟南芥T1植株(HvHPPDm-F383W-02)、转入HvHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(HvHPPDm-F372A-F383W-02)、转入ZmHPPD-02核苷酸序列的拟南芥T1植株(ZmHPPD-02)、转入ZmHPPDm-F372A-02核苷酸序列的拟南芥T1植株(ZmHPPDm-F372A-02)、转入ZmHPPDm-F383W-02核苷酸序列的拟南芥T1植株(ZmHPPDm-F383W-02)、转入ZmHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(ZmHPPDm-F372A-F383W-02)、转入PfHPPD-02核苷酸序列的拟南芥T1植株(PfHPPD-02)、转入PfHPPDm-F372A-02核苷酸序列的拟南芥T1植株(PfHPPDm-F372A-02)、转入PfHPPDm-F383W-02核苷酸序列的拟南芥T1植株(PfHPPDm-F383W-02)、转入PfHPPDm-F372A-F383W-02核苷酸序列的拟南芥T1植株(PfHPPDm-F372A-F383W-02)和转入对照重组表达载体DBN11726N的拟南芥T1植株(DBN11726N)。
按照上述第一实施例6中的方法分别将上述拟南芥T1植株和野生型拟南芥植株(CK)(播种后18天)分别用3种浓度的苯吡唑草酮(25g ai/ha(1倍大田浓度，1×)、100g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))、3种浓度的异噁唑草酮(35g ai/ha(0.5倍大田浓度，0.5×)、70g ai/ha(1倍大田浓度，1×)和0g ai/ha(水，0×))和3种浓度的甲基磺草酮(52.5g ai/ha(0.5倍大田浓度，0.5×)、105g ai/ha(1倍大田浓度，1×)和0g ai/ha(水，0×))进行喷洒以检测拟南芥的除草剂耐受性。实验结果如表4至表6所示。
表4、转入不同物种来源突变的HPPD的拟南芥T1植株对苯吡唑草酮的耐受性实验结果
表4的结果表明：(1)与转入未突变HPPD基因的拟南芥植株相比，转入不同物种来源的372位点和383位点组合突变(F372A+F383W)和转入不同物种来源的372单位点突变(F372A)的HPPD基因的拟南芥植株对苯吡唑草酮均具有不同程度的耐受性，仅部分物种来源(高粱、大麦和玉米)的383单位点突变(F383W)的HPPD基因可以赋予拟南芥植株对苯吡唑草酮的耐受性，而CK植株和对照载体DBN11726N植株则对苯吡唑草酮不具有耐受性。
(2)从抗性评价来看，对于苯吡唑草酮，转入不同物种来源(苜蓿除外)的372位点和383位点组合突变(F372A+F383W)的HPPD基因的拟南芥植株的除草剂耐受性不仅优于转入F372A或F383W单位点突变HPPD基因的拟南芥植株的除草剂耐受性，且进一步表现出协同增效的除草剂耐受性效果。
(3)从评分来看，转入来源于苜蓿的372位点和383位点组合突变(F372A+F383W)的HPPD基因的拟南芥植株的耐受性评分低于F372A或F383W单位点突变的评分；进一步的，对于4倍大田浓度的苯吡唑草酮，转入来源于苜蓿的372位点和383位点组合突变的HPPD基因的拟南芥植株中约有50％植株的损伤程度为0和1级，转入来源于苜蓿的372单位点突变的HPPD基因的拟南芥植株中有25％植株的损伤程度为0和1级，转入来源于苜蓿的383单位点突变的HPPD基因的拟南芥植株中损伤程度为0和1级的植株均为0。由此说明，来源于苜蓿的372位点和383位点组合突变(F372A+F383W)的HPPD基因可以赋予拟南芥植株协同增效的除草剂耐受性。
表5、转入不同物种来源突变的HPPD的拟南芥T1植株对异噁唑草酮的耐受性实验结果
表5的结果表明：(1)与转入未突变HPPD基因的拟南芥植株相比，转入不同物种来源的372位点和383位点组合突变(F372A+F383W)和转入不同物种来源的372单位点突变(F372A)的HPPD基因的拟南芥植株对异噁唑草酮均具有不同程度的耐受性，仅部分物种来源(高粱、大麦和玉米)的383单位点突变(F383W)的HPPD基因可以赋予拟南芥植株对异噁唑草酮的耐受性，而CK植株和对照载体DBN11726N植株则对异噁唑草酮不具有耐受性。
(2)从抗性评价来看，对于异噁唑草酮，转入不同物种来源(玉米除外)的372位点和383位点组合突变(F372A+F383W)的HPPD基因的拟南芥植株的除草剂耐受性不仅优于转入F372A或F383W单位点突变HPPD基因的拟南芥植株的除草剂耐受性，且进一步表现出协同增效的除草剂耐受性效果。
(3)从评分来看，转入来源于玉米的372位点和383位点组合突变的HPPD基因的拟南芥植株的耐受性评分低于F372A或F383W单位点突变的评分；进一步的，对于1倍大田浓度的异噁唑草酮，转入来源于玉米的372位点和383位点组合突变的HPPD基因的拟南芥植株中约有69％植株的损伤程度为0和1级，转入来源于玉米的372单位点突变的HPPD基因的拟南芥植株中有6％植株的损伤程度为0和1级，转入来源于玉米的383单位点突变的HPPD基因的拟南芥植株中损伤程度为0和1级的植株均为0。由此说明，来源于玉米的372位点和383位点组合突变(F372A+F383W)的HPPD基因可以赋予拟南芥植株协同增效的除草剂耐受性。
表6、转入不同物种来源突变的HPPD的拟南芥T1植株对甲基磺草酮的耐受性实验结果
表6的结果表明：(1)与转入未突变HPPD基因的拟南芥植株相比，转入不同物种来源的372位点和383位点组合突变(F372A+F383W)或转入不同物种来源的372单位点突变(F372A)的HPPD基因的拟南芥植株对甲基磺草酮均具有不同程度的耐受性，仅部分物种来源(大麦和玉米)的383单位点突变(F383W)的HPPD基因可以赋予拟南芥植株对甲基磺草酮的耐受性，而CK植株和对照载体DBN11726N植株则对甲基磺草酮不具有耐受性。
(2)从抗性评价来看，对于甲基磺草酮，转入不同物种来源(烟草来源的除外)的372位点和383位点组合突变(F372A+F383W)的HPPD基因的拟南芥植株的除草剂耐受性不仅优于转入F372A或F383W单位点突变HPPD基因的拟南芥植株的除草剂耐受性，且进一步表现出协同增效的除草剂耐受性效果。
(3)从评分来看，对于0.5倍和1倍大田浓度的甲基磺草酮，转入来源于烟草的372位点和383位点组合突变(F372A+F383W)的HPPD基因的拟南芥植株的耐受性评分均低于F372A或F383W单位点突变的评分，且进一步表现出协同增效的除草剂耐受性效果。
综上，表4至表6的结果表明：不同物种来源的372位点和383位点组合突变(F372A+F383W)的HPPD基因均可以赋予植物协同增效的除草剂耐受性效果。
第三实施例、对HPPD氨基酸序列的372位点和383位点进行不同形式的突变(F372G+F383W组合突变或F372V+F383W组合突变)并验证其突变效果
1、获得AsHPPDm-F372G-F383W和AsHPPDm-F372V-F383W基因
(1)将所述AsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为甘氨酸(G)，获得AsHPPDm-F372G氨基酸序列，如序列表中SEQ ID NO：155所示，编码所述AsHPPDm-F372G氨基酸序列的AsHPPDm-F372G-01核苷酸序列，如序列表中SEQ ID NO：156所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F372G氨基酸序列的AsHPPDm-F372G-02核苷酸序列，如序列表中SEQ ID NO：157所示。
将所述AsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为甘氨酸(G)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AsHPPDm-F372G-F383W氨基酸序列，如序列表中SEQ ID NO：158所示，编码所述AsHPPDm-F372G-F383W氨基酸序列的AsHPPDm-F372G-F383W-01核苷酸序列，如序列表中SEQ ID NO：159所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F372G-F383W氨基酸序列的AsHPPDm-F372G-F383W-02核苷酸序列，如序列表中SEQ ID NO：160所示。
(2)将所述AsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为缬氨酸(V)，获得AsHPPDm-F372V氨基酸序列，如序列表中SEQ ID NO：161所示，编码所述AsHPPDm-F372V氨基酸序列的AsHPPDm-F372V-01核苷酸序列，如序列表中SEQ ID NO：162所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F372V氨基酸序列的AsHPPDm-F372V-02核苷酸序列，如序列表中SEQ ID NO：163所示。
将所述AsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为缬氨酸(V)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AsHPPDm-F372V-F383W氨基酸序列，如序列表中SEQ ID NO：164所示，编码所述AsHPPDm-F372V-F383W氨基酸序列的AsHPPDm-F372V-F383W-01核苷酸序列，如序列表中SEQ ID NO：165所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F372V-F383W氨基酸序列的AsHPPDm-F372V-F383W-02核苷酸序列，如序列表中SEQ ID NO：166所示。
2、构建含有燕麦HPPD基因(F372G+F383W或F372V+F383W)的拟南芥重组表达载体
按照上述第一实施例3中构建含有AsHPPDm-F372A-F383W-02核苷酸序列的重组表达载体DBN11726的方法，将连接所述通用接头引物1的所述AsHPPDm-F372G-02核苷酸序列、AsHPPDm-F372G-F383W-02核苷酸序列、AsHPPDm-F372V-02核苷酸序列和AsHPPDm-F372V-F383W-02核苷酸序列分别与所述线性化的DBNBC-01表达载体骨架进行重组反应，依次得到重组表达载体DBN11774至DBN11777。测序验证重组表达载体DBN11774至DBN11777中上述核苷酸序列正确插入。
3、拟南芥重组表达载体转化农杆菌
按照上述第一实施例4中拟南芥重组表达载体转化农杆菌的方法，将己经构建正确的重组表达载体DBN11774至DBN11777以及第一实施例3中含有所述AsHPPD-02核苷酸序列的重组表达载体DBN11727、第一实施例3中含有所述AsHPPDm-F383W-02核苷酸序列的重组表达载体DBN11729和第一实施例3中构建的对照重组表达载体DBN11726N分别用液氮法转化到农杆菌GV3101中，测序验证结果表明重组表达载体DBN11774至DBN11777以及DBN11727、DBN11729和DBN11726N的结构完全正确。
4、转入AsHPPDm-F372G-F383W-02或AsHPPDm-F372V-F383W-02核苷酸序列的转基因拟南芥植株的除草剂耐受性效果检测
按照上述第一实施例5中的方法将拟南芥花序浸泡在本实施例3的所述农杆菌菌液中，以将本实施例2中构建的重组表达载体DBN11774至DBN11777以及第一实施例3中含有所述AsHPPD-02核苷酸序列的重组表达载体DBN11727、第一实施例3中含有所述AsHPPDm-F383W-02核苷酸序列的重组表达载体DBN11729和第一实施例3中构建的对照重组表达载体DBN11726N中的T-DNA转入到拟南芥染色体中，获得相应的转基因拟南芥植株，即转入AsHPPDm-F372G-02核苷酸序列的拟南芥T1植株(AsHPPDm-F372G-02)、转入AsHPPDm-F372G-F383W-02核苷酸序列的拟南芥T1植株(AsHPPDm-F372G-F383W-02)、转入AsHPPDm-F372V-02核苷酸序列的拟南芥T1植株(AsHPPDm-F372V-02)、转入AsHPPDm-F372V-F383W-02核苷酸序列的拟南芥T1植株(AsHPPDm-F372V-F383W-02)、转入AsHPPD-02核苷酸序列的拟南芥T1植株、转入AsHPPDm-F383W-02核苷酸序列的拟南芥T1植株和转入对照重组表达载体DBN11726N拟南芥T1植株。
5、验证F372G+F383W或F372V+F383W的协同效果
按照上述第一实施例6中的方法分别将上述拟南芥T1植株和野生型拟南芥植株(CK)(播种后18天)分别用3种浓度的苯吡唑草酮(100g ai/ha(4倍大田浓度，4×)、200gai/ha(8倍大田浓度，8×)和0g ai/ha(水，0×))、3种浓度的异噁唑草酮(140g ai/ha(2倍大田浓度，2×)、280g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))和3种浓度的甲基磺草酮(210g ai/ha(2倍大田浓度，2×)、420g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))进行喷洒以检测拟南芥的除草剂耐受性。实验结果如表7至表9所示。
表7、转基因拟南芥T1植株对苯吡唑草酮的耐受性实验结果
表7的结果表明：相比于CK，对于4倍或8倍大田浓度的苯吡唑草酮，AsHPPDm-F372G-02、AsHPPDm-F383W-02、AsHPPDm-F372G-F383W-02、AsHPPDm-F372V-02和AsHPPDm-F372V-F383W-02全部表现出高抗的耐受性，而AsHPPD-02和对照载体DBN11726N植株对苯吡唑草酮不具有耐受性。
表8、转基因拟南芥T1植株对异噁唑草酮的耐受性实验结果
表9、转基因拟南芥T1植株对甲基磺草酮的耐受性实验结果
表8和表9的结果表明：(1)相比于CK，转入燕麦来源的372位点和383位点组合突变(F372G+F383W或F372V+F383W)的HPPD基因、转入燕麦来源的372单位点突变(F372G或F372V)的HPPD基因和转入燕麦来源的383单位点突变(F383W)的HPPD基因的拟南芥植株对异噁唑草酮和甲基磺草酮均具有不同程度的耐受性，而转入未突变HPPD基因的拟南芥植株和转入对照载体DBN11726N的植株对异噁唑草酮和甲基磺草酮均不具有耐受性。
(2)从抗性评价来看，对于2倍大田浓度的异噁唑草酮或甲基磺草酮，转入燕麦来源的372位点和383位点组合突变(F372G+F383W或F372V+F383W)HPPD基因的拟南芥植株的除草剂耐受性(高抗)优于转入372单位点突变(中抗)或383单位点突变(中抗)HPPD基因的拟南芥植株的除草剂耐受性；对于4倍大田浓度的异噁唑草酮或甲基磺草酮，转入燕麦来源的372位点和383位点组合突变(F372G+F383W或F372V+F383W)HPPD基因的拟南芥植株的除草剂耐受性(高抗)不仅优于转入372单位点突变(低抗)或383单位点突变(低抗)HPPD基因的拟南芥植株的除草剂耐受性，且进一步表现出协同增效的除草剂耐受性效果。
上述表8和表9充分说明对野生型HPPD氨基酸序列的372位点和383位点进行不同形式的突变(F372G+F383W组合突变或F372V+F383W组合突变)亦具有协同增效的HPPD抑制剂除草剂耐受性效果。
第四实施例、HPPD氨基酸序列的372位点+383位点的组合突变与其他突变位点进一步组合及其突变效果
1、获得多位点组合突变序列
(1)获得HPPDm-1氨基酸序列(AsHPPDm-A107-F372A-F383W氨基酸序列)
将所述AsHPPD氨基酸序列的107位点由原来的丙氨酸(A)进行缺失突变同时将372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得AsHPPDm-A107-F372A氨基酸序列，如序列表中SEQ ID NO：167所示，编码所述AsHPPDm-A107-F372A氨基酸序列的AsHPPDm-A107-F372A-01核苷酸序列，如序列表中SEQ ID NO：168所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-A107-F372A氨基酸序列的AsHPPDm-A107-F372A-02核苷酸序列，如序列表中SEQ ID NO：169所示。
将所述AsHPPD氨基酸序列的107位点由原来的丙氨酸(A)进行缺失突变同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AsHPPDm-A107-F383W氨基酸序列，如序列表中SEQ ID NO：170所示，编码所述AsHPPDm-A107-F383W氨基酸序列的AsHPPDm-A107-F383W-01核苷酸序列，如序列表中SEQ ID NO：171所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-A107-F383W氨基酸序列的AsHPPDm-A107-F383W-02核苷酸序列，如序列表中SEQ ID NO：172所示。
将所述AsHPPD氨基酸序列的107位点由原来的丙氨酸(A)进行缺失突变，372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得HPPDm-1氨基酸序列(AsHPPDm-A107-F372A-F383W氨基酸序列)，如序列表中SEQID NO：173所示，编码所述HPPDm-1氨基酸序列的HPPDm-1-01核苷酸序列，如序列表中SEQID NO：174所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HPPDm-1氨基酸序列的HPPDm-1-02核苷酸序列，如序列表中SEQ ID NO：175所示。
(2)获得HPPDm-2氨基酸序列(AsHPPDm-A111T-F372A-F383W氨基酸序列)
将所述AsHPPD氨基酸序列的111位点由原来的丙氨酸(A)突变为苏氨酸(T)同时将372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)，获得AsHPPDm-A111T-F372A氨基酸序列，如序列表中SEQ ID NO：176所示，编码所述AsHPPDm-A111T-F372A氨基酸序列的AsHPPDm-A111T-F372A-01核苷酸序列，如序列表中SEQ ID NO：177所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-A111T-F372A氨基酸序列的AsHPPDm-A111T-F372A-02核苷酸序列，如序列表中SEQ ID NO：178所示。
将所述AsHPPD氨基酸序列的111位点由原来的丙氨酸(A)突变为苏氨酸(T)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AsHPPDm-A111T-F383W氨基酸序列，如序列表中SEQ ID NO：179所示，编码所述AsHPPDm-A111T-F383W氨基酸序列的AsHPPDm-A111T-F383W-01核苷酸序列，如序列表中SEQ ID NO：180所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-A111T-F383W氨基酸序列的AsHPPDm-A111T-F383W-02核苷酸序列，如序列表中SEQ ID NO：181所示。
将所述AsHPPD氨基酸序列的111位点由原来的丙氨酸(A)突变为苏氨酸(T)，372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得HPPDm-2氨基酸序列(AsHPPDm-A111T-F372A-F383W氨基酸序列)，如序列表中SEQ ID NO：182所示，编码所述HPPDm-2氨基酸序列的HPPDm-2-01核苷酸序列，如序列表中SEQ ID NO：183所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HPPDm-2氨基酸序列的HPPDm-2-02核苷酸序列，如序列表中SEQ ID NO：184所示。
(3)获得HPPDm-3氨基酸序列(AsHPPDm-A106G-F372A-F383W氨基酸序列)
将所述AsHPPD氨基酸序列的106位点由原来的丙氨酸(A)突变为甘氨酸(G)，372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得HPPDm-3氨基酸序列(AsHPPDm-A106G-F372A-F383W氨基酸序列)，如序列表中SEQ ID NO：185所示，编码所述HPPDm-3氨基酸序列的HPPDm-3-01核苷酸序列，如序列表中SEQ ID NO：186所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HPPDm-3氨基酸序列的HPPDm-3-02核苷酸序列，如序列表中SEQ ID NO：187所示。
(4)获得HPPDm-4氨基酸序列(AsHPPDm-A107-K351N-F372A-F383W氨基酸序列)
将所述AsHPPD氨基酸序列的107位点由原来的丙氨酸(A)进行缺失突变，将351位点由原来的赖氨酸(K)突变为天冬酰胺(N)，372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得HPPDm-4氨基酸序列(AsHPPDm-A107-K351N-F372A-F383W氨基酸序列)，如序列表中SEQ ID NO：188所示，编码所述HPPDm-4氨基酸序列的HPPDm-4-01核苷酸序列，如序列表中SEQ ID NO：189所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HPPDm-4氨基酸序列的HPPDm-4-02核苷酸序列，如序列表中SEQID NO：190所示。
(5)获得HPPDm-5氨基酸序列(AsHPPDm-A111T-K351N-F372A-F383W氨基酸序列)
将所述AsHPPD氨基酸序列的111位点由原来的丙氨酸(A)突变为苏氨酸(T)，将351位点由原来的赖氨酸(K)突变为天冬酰胺(N)，372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得HPPDm-5氨基酸序列(AsHPPDm-A111T-K351N-F372A-F383W氨基酸序列)，如序列表中SEQ ID NO：191所示，编码所述HPPDm-5氨基酸序列的HPPDm-5-01核苷酸序列，如序列表中SEQ ID NO：192所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HPPDm-5氨基酸序列的HPPDm-5-02核苷酸序列，如序列表中SEQID NO：193所示。
(6)获得HPPDm-6氨基酸序列(AsHPPDm-A106G-K351N-F372A-F383W氨基酸序列)
将所述AsHPPD氨基酸序列的106位点由原来的丙氨酸(A)突变为甘氨酸(G)，将351位点由原来的赖氨酸(K)突变为天冬酰胺(N)，372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得HPPDm-6氨基酸序列(AsHPPDm-A106G-K351N-F372A-F383W氨基酸序列)，如序列表中SEQ ID NO：194所示，编码所述HPPDm-6氨基酸序列的HPPDm-6-01核苷酸序列，如序列表中SEQ ID NO：195所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述HPPDm-6氨基酸序列的HPPDm-6-02核苷酸序列，如序列表中SEQID NO：196所示。
(7)获得HPPDm-7氨基酸序列
HPPDm-7的氨基酸序列为在所述AsHPPDm-A107-K351N-F372A-F383W氨基酸序列的基础上将燕麦源HPPD氨基酸序列的C端氨基酸进行突变获得，所述HPPDm-7的氨基酸序列，如序列表中SEQ ID NO：197所示，编码所述HPPDm-7氨基酸序列的HPPDm-7-01核苷酸序列，如序列表中SEQ ID NO：198所示，依据拟南芥/大豆共同偏好性密码子获得编码所述HPPDm-7氨基酸序列的HPPDm-7-02核苷酸序列，如序列表中SEQ ID NO：199所示。
(8)获得HPPDm-8氨基酸序列
HPPDm-8的氨基酸序列为在所述AsHPPDm-A111T-K351N-F372A-F383W氨基酸序列的基础上将燕麦源HPPD氨基酸序列的C端氨基酸进行突变获得，所述HPPDm-8的氨基酸序列，如序列表中SEQ ID NO：200所示，所述编码所述HPPDm-8氨基酸序列的HPPDm-8-01核苷酸序列，如序列表中SEQ ID NO：201所示，依据拟南芥/大豆共同偏好性密码子获得编码所述HPPDm-8氨基酸序列的HPPDm-8-02核苷酸序列，如序列表中SEQ ID NO：202所示。
(9)获得HPPDm-9氨基酸序列
HPPDm-9的氨基酸序列为在所述AsHPPDm-A106G-K351N-F372A-F383W氨基酸序列的基础上将燕麦源HPPD氨基酸序列的C端氨基酸进行突变获得，所述HPPDm-9的氨基酸序列，如序列表中SEQ ID NO：203所示，所述编码所述HPPDm-9氨基酸序列的HPPDm-9-01核苷酸序列，如序列表中SEQ ID NO：204所示，依据拟南芥/大豆共同偏好性密码子获得编码所述HPPDm-9氨基酸序列的HPPDm-9-02核苷酸序列，如序列表中SEQ ID NO：205所示。
2、构建含有多位点组合突变HPPD的拟南芥重组表达载体
按照上述第一实施例3中构建含有AsHPPDm-F372A-F383W-02核苷酸序列的重组表达载体DBN11726的方法，将连接所述通用接头引物1的所述AsHPPDm-A107-F372A-02核苷酸序列、AsHPPDm-A107-F383W-02核苷酸序列、HPPDm-1-02核苷酸序列、AsHPPDm-A111T-F372A-02核苷酸序列、AsHPPDm-A111T-F383W-02核苷酸序列、HPPDm-2-02核苷酸序列、HPPDm-3-02核苷酸序列、HPPDm-4-02核苷酸序列、HPPDm-5-02核苷酸序列、HPPDm-6-02核苷酸序列、HPPDm-7-02核苷酸序列、HPPDm-8-02核苷酸序列和HPPDm-9-02核苷酸序列分别与所述线性化的DBNBC-01表达载体骨架进行重组反应，依次得到重组表达载体DBN11778至DBN11790。测序验证重组表达载体DBN11778至DBN11790中上述核苷酸序列正确插入。
3、拟南芥重组表达载体转化农杆菌
按照上述第一实施例4中拟南芥重组表达载体转化农杆菌的方法，将已经构建正确的重组表达载体DBN11778至DBN11790以及第一实施例3中含有AsHPPD-02核苷酸序列的重组表达载体DBN11727和第一实施例3中对照表达载体DBN11726N分别用液氮法转化到农杆菌GV3101中，测序验证，结果表明重组表达载体DBN11778至DBN11790以及DBN11727和DBN11726N的结构完全正确。
4、转入多位点组合突变HPPD的拟南芥植株的除草剂耐受性效果检测
按照上述第一实施例5中的方法将拟南芥花序浸泡在本实施例3的所述农杆菌菌液中，以将本实施例2中构建的重组表达载体DBN11778至DBN11790以及第一实施例3中含有AsHPPD-02核苷酸序列的重组表达载体DBN11727和第一实施例3中对照表达载体DBN11726N中的T-DNA转入到拟南芥染色体中，获得相应的转基因拟南芥植株，即转入AsHPPDm-A107-F372A-02核苷酸序列的拟南芥T1植株(AsHPPDm-A107-F372A-02)、转入AsHPPDm-A107-F383W-02核苷酸序列的拟南芥T1植株(AsHPPDm-A107-F383W-02)、转入HPPDm-1-02核苷酸序列的拟南芥T1植株(HPPDm-1-02)、转入AsHPPDm-A111T-F372A-02核苷酸序列的拟南芥T1植株(AsHPPDm-A111T-F372A-02)、转入AsHPPDm-A111T-F383W-02核苷酸序列的拟南芥T1植株(AsHPPDm-A111T-F383W-02)、转入HPPDm-2-02核苷酸序列的拟南芥T1植株(HPPDm-2-02)、转入HPPDm-3-02核苷酸序列的拟南芥T1植株(HPPDm-3-02)、转入HPPDm-4-02核苷酸序列的拟南芥T1植株(HPPDm-4-02)、转入HPPDm-5-02核苷酸序列的拟南芥T1植株(HPPDm-5-02)、转入HPPDm-6-02核苷酸序列的拟南芥T1植株(HPPDm-6-02)、转入HPPDm-7-02核苷酸序列的拟南芥T1植株(HPPDm-7-02)、转入HPPDm-8-02核苷酸序列的拟南芥T1植株(HPPDm-8-02)和转入HPPDm-9-02核苷酸序列的拟南芥T1植株(HPPDm-9-02)、转入AsHPPD-02核苷酸序列的拟南芥T1植株(AsHPPD-02)和转入对照表达载体的拟南芥T1植株(DBN11726N)。
按照上述第一实施例6中的方法分别将上述拟南芥T1植株和野生型拟南芥植株(CK)(播种后18天)分别用3种浓度的苯吡唑草酮(100g ai/ha(4倍大田浓度，4×)、200gai/ha(8倍大田浓度，8×)和0g ai/ha(水，0×))、3种浓度的异噁唑草酮(140g ai/ha(2倍大田浓度，2×)、280g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))和3种浓度的甲基磺草酮(210g ai/ha(2倍大田浓度，2×)、420g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))进行喷洒以检测拟南芥的除草剂耐受性。实验结果如表10至表12所示。
表10、转入多位点组合突变HPPD基因的拟南芥T1植株对苯吡唑草酮的耐受性实验结果
表10的结果表明：相比于CK和转入未突变HPPD基因的拟南芥植株，对于4倍或8倍大田浓度的苯吡唑草酮，在372位点和383位点组合突变的基础上结合其他突变位点(包括A107缺失、A111T、A106G、A107+K351N、A111T+K351N、A106G+K351N、A107+K351N+C端突变位点、A111T+K351N+C端突变位点或A106G+K351N+C端突变位点)的HPPD基因均可以赋予植物对苯吡唑草酮高抗的耐受性，表明HPPD氨基酸序列的372位点+383位点组合突变结合其他突变位点没有影响单独372位点+383位点组合突变对苯吡唑草酮的耐受性，同时证明了HPPD氨基酸序列的372位点和383位点组合突变赋予植物对HPPD抑制剂类除草剂耐受性的重要性和稳定性。而转入对照重组表达载体DBN11726N的拟南芥T1植株对苯吡唑草酮不具有耐受性。
表11、转入多位点组合突变HPPD基因的拟南芥T1植株对异噁唑草酮的耐受性实验结果
表12、转入多位点组合突变HPPD的拟南芥T1植株对甲基磺草酮的耐受性实验结果
表11至表12的结果表明：(1)相比于CK和转入未突变HPPD基因的拟南芥植株，对于2倍或4倍大田浓度的异噁唑草酮和甲基磺草酮，在372位点和383位点组合突变的基础上结合其他突变位点(包括A107缺失、A111T、A106G、A107+K351N、A111T+K351N、A106G+K351N、A107+K351N+C端突变位点、A111T+K351N+C端突变位点或A106G+K351N+C端突变位点)的HPPD基因均可赋予植物对异噁唑草酮和甲基磺草酮高抗的耐受性；而转入对照重组表达载体DBN11726N的拟南芥T1植株对异噁唑草酮和甲基磺草酮不具有耐受性。
(2)从抗性评价来看，对于4倍大田浓度的异噁唑草酮或甲基磺草酮，转入HPPDm-1-02(AsHPPDm-A107-F372A-F383W-02)基因的拟南芥植株的除草剂耐受性(高抗)不仅优于转入AsHPPDm-A107-F372A-02基因(低抗)或转入AsHPPDm-A107-F383W-02基因(低抗)的拟南芥植株的除草剂耐受性，且进一步表现出协同增效的除草剂耐受性效果；同理，转入HPPDm-2-02(AsHPPDm-A111T-F372A-F383W基因的拟南芥植株的除草剂耐受性(高抗)不仅优于转入AsHPPDm-A111T-F372A-02基因(低抗)或转入AsHPPDm-A111T-F383W-02基因(低抗)的拟南芥植株的除草剂耐受性，且进一步表现出协同增效的除草剂耐受性效果。因此，HPPD氨基酸序列的372位点+383位点组合突变结合其他突变位点没有影响单独372位点+383位点组合突变对HPPD抑制剂类除草剂的协同增效的除草剂耐受性效果，也进一步证明了HPPD氨基酸序列的372位点+383位点组合突变赋予植物对HPPD抑制剂类除草剂耐受性的重要性和稳定性。
(3)从抗性评分来看，对于4倍大田浓度的异噁唑草酮，转入HPPDm-7-02至HPPDm-9-02核苷酸序列的拟南芥T1植株的抗性评分(0分)均优于转入HPPDm-1-02至HPPDm-6-02核苷酸序列的拟南芥T1植株的抗性评分，表明优化HPPD氨基酸序列的C端对提高植株对异噁唑草酮的耐受性将是有益的。
第五实施例、对HPPD氨基酸序列进行非F372A(F372G/F372V)+F383W组合形式突变并验证其突变效果
1、获得来源于燕麦和拟南芥HPPD氨基酸序列的非F372A(F372G/F372V)+F383W组合形式的突变基因
(1)燕麦来源HPPD的F372A+F415W组合突变基因
将所述AsHPPD氨基酸序列的415位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AsHPPDm-F415W氨基酸序列，如序列表中SEQ ID NO：206所示，编码所述AsHPPDm-F415W氨基酸序列的AsHPPDm-F415W-01核苷酸序列，如序列表中SEQ ID NO：207所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F415W氨基酸序列的AsHPPDm-F415W-02核苷酸序列，如序列表中SEQ ID NO：208所示。
将所述AsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将415位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AsHPPDm-F372A-F415W氨基酸序列，如序列表中SEQ ID NO：209所示，编码所述AsHPPDm-F372A-F415W氨基酸序列的AsHPPDm-F372A-F415W-01核苷酸序列，如序列表中SEQ ID NO：210所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F372A-F415W氨基酸序列的AsHPPDm-F372A-F415W-02核苷酸序列，如序列表中SEQ ID NO：211所示。
(2)拟南芥来源HPPD的F372A+F415W组合突变基因
将所述AtHPPD氨基酸序列的第424位氨基酸(对应于SEQ ID NO：1所示氨基酸序列的第415位，即415位点)由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AtHPPDm-F415W氨基酸序列，如序列表中SEQ ID NO：212所示，编码所述AtHPPDm-F415W氨基酸序列的AtHPPDm-F415W-01核苷酸序列，如序列表中SEQ ID NO：213所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AtHPPDm-F415W氨基酸序列的AtHPPDm-F415W-02核苷酸序列，如序列表中SEQ ID NO：214所示。
将所述AtHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将415位点由原来的苯丙氨酸(F)突变为色氨酸(W)，获得AtHPPDm-F372A-F415W氨基酸序列，如序列表中SEQ ID NO：215所示，编码所述AtHPPDm-F372A-F415W氨基酸序列的AtHPPDm-F372A-F415W-01核苷酸序列，如序列表中SEQ ID NO：216所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AtHPPDm-F372A-F415W氨基酸序列的AtHPPDm-F372A-F415W-02核苷酸序列，如序列表中SEQ ID NO：217所示。
(3)燕麦来源HPPD的F372A+F383Y组合突变基因
将所述AsHPPD氨基酸序列的383位点由原来的苯丙氨酸(F)突变为酪氨酸(Y)，获得AsHPPDm-F383Y氨基酸序列，如序列表中SEQ ID NO：218所示，编码所述AsHPPDm-F383Y氨基酸序列的AsHPPDm-F383Y-01核苷酸序列，如序列表中SEQ ID NO：219所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F383Y氨基酸序列的AsHPPDm-F383Y-02核苷酸序列，如序列表中SEQ ID NO：220所示。
将所述AsHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为酪氨酸(Y)，获得AsHPPDm-F372A-F383Y氨基酸序列，如序列表中SEQ ID NO：221所示，编码所述AsHPPDm-F372A-F383Y氨基酸序列的AsHPPDm-F372A-F383Y-01核苷酸序列，如序列表中SEQ ID NO：222所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AsHPPDm-F372A-F383Y氨基酸序列的AsHPPDm-F372A-F383Y-02核苷酸序列，如序列表中SEQ ID NO：223所示。
(4)拟南芥来源HPPD的F372A+F383Y组合突变基因
将所述AtHPPD氨基酸序列的第392位氨基酸(对应于SEQ ID NO：1所示氨基酸序列的第383位，即383位点)由原来的苯丙氨酸(F)突变为酪氨酸(Y)，获得AtHPPDm-F383Y氨基酸序列，如序列表中SEQ ID NO：224所示，编码所述AtHPPDm-F383Y氨基酸序列的AtHPPDm-F383Y-01核苷酸序列，如序列表中SEQ ID NO：225所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AtHPPDm-F383Y氨基酸序列的AtHPPDm-F383Y-02核苷酸序列，如序列表中SEQ ID NO：226所示。
将所述AtHPPD氨基酸序列的372位点由原来的苯丙氨酸(F)突变为丙氨酸(A)同时将383位点由原来的苯丙氨酸(F)突变为酪氨酸(Y)，获得AtHPPDm-F372A-F383Y氨基酸序列，如序列表中SEQ ID NO：227所示，编码所述AtHPPDm-F372A-F383Y氨基酸序列的AtHPPDm-F372A-F383Y-01核苷酸序列，如序列表中SEQ ID NO：228所示，依据拟南芥/大豆共同偏好性密码子获得的编码所述AtHPPDm-F372A-F383Y氨基酸序列的AtHPPDm-F372A-F383Y-02核苷酸序列，如序列表中SEQ ID NO：229所示。
2、构建含有非F372A(F372G/F372V)+F383W组合形式的突变HPPD基因的拟南芥重组表达载体
按照上述第一实施例3中构建含有AsHPPDm-F372A-F383W-02核苷酸序列的重组表达载体DBN11726的方法，将连接所述通用接头引物1的所述AsHPPDm-F415W-02核苷酸序列、AsHPPDm-F372A-F415W-02核苷酸序列、AtHPPDm-F415W-02核苷酸序列、AtHPPDm-F372A-F415W-02核苷酸序列、AsHPPDm-F383Y-02核苷酸序列、AsHPPDm-F372A-F383Y-02核苷酸序列、AtHPPDm-F383Y-02核苷酸序列和AtHPPDm-F372A-F383Y-02核苷酸序列分别与所述线性化的DBNBC-01表达载体骨架进行重组反应，依次得到重组表达载体DBN11791至DBN11798。测序验证重组表达载体DBN11791至DBN11798中上述核苷酸序列正确插入。
3、拟南芥重组表达载体转化农杆菌
按照上述第一实施例4中拟南芥重组表达载体转化农杆菌的方法，将已经构建正确的重组表达载体DBN11791至DBN11798，第一实施例3中含有AsHPPD-02核苷酸序列的重组表达载体DBN11727，第一实施例3中含有AsHPPDm-F372A-02核苷酸序列的重组表达载体DBN11728，第二实施例2中含有AtHPPDm-02核苷酸序列的重组表达载体DBN11730和第二实施例2中含有AtHPPDm-F372A-02核苷酸序列的重组表达载体DBN11731分别用液氮法转化到农杆菌GV3101中，测序验证结果表明重组表达载体DBN11791至DBN11798，DBN11727，DBN11728，DBN11730和DBN11731的结构完全正确。
4、转入非F372A(F372G/F372V)+F383W组合形式的突变HPPD基因的拟南芥植株的除草剂耐受性效果检测
按照上述第一实施例5中的方法将拟南芥花序浸泡在本实施例3的所述农杆菌菌液中，以将本实施例2中构建的重组表达载体DBN11791至DBN11798以及DBN11727，DBN11728，DBN11730和DBN11731中的T-DNA转入到拟南芥染色体中，获得相应的转基因拟南芥植株，即转入AsHPPDm-F415W-02核苷酸序列的拟南芥T1植株(AsHPPDm-F415W-02)、转入AsHPPDm-F372A-F415W-02核苷酸序列的拟南芥T1植株(AsHPPDm-F372A-F415W-02)、转入AtHPPDm-F415W-02核苷酸序列的拟南芥T1植株(AtHPPDm-F415W-02)、转入AtHPPDm-F372A-F415W-02核苷酸序列的拟南芥T1植株(AtHPPDm-F372A-F415W-02)、转入AsHPPDm-F383Y-02核苷酸序列的拟南芥T1植株(AsHPPDm-F383Y-02)、转入AsHPPDm-F372A-F383Y-02核苷酸序列的拟南芥T1植株(AsHPPDm-F372A-F383Y-02)、转入AtHPPDm-F383Y-02核苷酸序列的拟南芥T1植株(AtHPPDm-F383Y-02)、转入AtHPPDm-F372A-F383Y-02核苷酸序列的拟南芥T1植株(AtHPPDm-F372A-F383Y-02)、转入AsHPPD-02核苷酸序列的拟南芥T1植株(AsHPPD-02)、转入AsHPPDm-F372A-02核苷酸序列的拟南芥T1植株(AsHPPDm-F372A-02)、转入AtHPPDm-02核苷酸序列的拟南芥T1植株(AtHPPDm-02)和转入AtHPPDm-F372A-02核苷酸序列的拟南芥T1植株(AtHPPDm-F372A-02)。
按照上述第一实施例6中的方法分别将上述拟南芥T1植株和野生型拟南芥植株(播种后18天)分别用3种浓度的苯吡唑草酮(25g ai/ha(1倍大田浓度，1×)、100g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))、5种浓度的异噁唑草酮(35g ai/ha(0.5倍大田浓度，0.5×)、70g ai/ha(1倍大田浓度，1×)、140g ai/ha(2倍大田浓度，2×)、280g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))(其中，0×、0.5×和1×浓度的异噁唑草酮用于喷洒转入来源于拟南芥突变HPPD的拟南芥T1植株；0×、2×和4×浓度的异噁唑草酮用于喷洒转入来源于燕麦突变HPPD的拟南芥T1植株)和5种浓度的甲基磺草酮(52.5g ai/ha(0.5倍大田浓度，0.5×)、105g ai/ha(1倍大田浓度，1×)、210g ai/ha(2倍大田浓度，2×)、420gai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))(其中，0×、0.5×和1×浓度的甲基磺草酮用于喷洒转入来源于拟南芥突变HPPD的拟南芥T1植株；0×、2×和4×浓度的甲基磺草酮用于喷洒转入来源于燕麦突变HPPD的拟南芥T1植株)进行喷洒以检测拟南芥的除草剂耐受性。实验结果如表13至表15所示。
表13非F372A(F372G/F372V)+F383W组合形式突变HPPD的拟南芥T1植株对苯吡唑草酮的耐受性实验结果
表14非F372A(F372G/F372V)+F383W组合形式突变HPPD的拟南芥T1植株对异噁唑草酮的耐受性实验结果
表15非F372A(F372G/F372V)+F383W组合形式突变HPPD的拟南芥T1植株对甲基磺草酮的耐受性实验结果
表13至表15的结果表明：转入F372A+F415W或F372A+F383Y组合突变HPPD基因的拟南芥T1植株与转入F372A单位点突变HPPD基因的拟南芥T1植株对HPPD抑制剂除草剂的耐受性程度基本无差别。由此可见，将HPPD氨基酸序列中的任意两个位点进行任意形式的组合突变并非均会赋予植物对HPPD抑制剂除草剂协同增效的耐受性效果，同时亦说明本发明HPPD氨基酸序列的372位点+383位点突变所产生的协同增效的技术效果是难以预料的。
第六实施例、转基因大豆植株的获得和验证
1、重组表达载体转化农杆菌
分别将第二实施例2中含有所述SbHPPD-02核苷酸序列的重组表达载体DBN11758、含有所述SbHPPDm-F372A-02核苷酸序列的重组表达载体DBN11759、含有所述SbHPPDm-F383W-02核苷酸序列的重组表达载体DBN11760和含有所述SbHPPDm-F372A-F383W-02核苷酸序列的重组表达载体DBN11761以及第一实施例3中的对照重组表达载体DBN11726N分别用液氮法转化到农杆菌LBA4404(Invitrgen，Chicago，USA，CAT：18313-015)中，其转化条件为：100μL农杆菌LBA4404、3μL质粒DNA(重组表达载体)；置于液氮中10min，37℃温水浴10min；将转化后的农杆菌LBA4404接种于LB试管中于温度28℃、转速为200rpm条件下培养2h，涂于含50mg/L的利福平(Rifampicin)和50mg/L的壮观霉素的所述LB固体平板上直至长出阳性单克隆，挑取单克隆培养并提取其质粒，将提取的质粒进行测序鉴定，结果表明重组表达载体DBN11758、DBN11759、DBN11760、DBN11761和DBN11726N结构完全正确。
2、获得转基因大豆植株
按照常规采用的农杆菌侵染法，将无菌培养的大豆品种中黄13的子叶节组织与本实施例1中所述的农杆菌共培养，以将上述重组表达载体DBN11758、DBN11759、DBN11760、DBN11761和DBN11726N中的T-DNA(包括玄参花叶病毒的34S增强子序列、油菜真核延长因子基因1α(Tsf1)启动子序列、拟南芥叶绿体转运肽序列、5-烯醇丙酮酸莽草酸-3-磷酸合酶基因、豌豆RbcS基因的终止子序列、拟南芥泛素(Ubiquitin)10基因启动子序列、SbHPPD-02核苷酸序列、SbHPPDm-F372A-02核苷酸序列、SbHPPDm-F383W-02核苷酸序列、SbHPPDm-F372A-F383W-02核苷酸序列、胭脂碱合成酶基因的终止子序列、花椰菜花叶病毒35S启动子序列、膦丝菌素N-乙酰基转移酶基因、花椰菜花叶病毒35S终止子序列)转入到大豆染色体组中，获得了转入SbHPPD-02核苷酸序列的大豆植株、转入SbHPPDm-F372A-02核苷酸序列的大豆植株、转入SbHPPDm-F383W-02核苷酸序列的大豆植株、转入SbHPPDm-F372A-F383W-02核苷酸序列的大豆植株和转入对照载体DBN11726N的大豆植株。
对于农杆菌介导的大豆转化，简要地，将成熟的大豆种子在大豆萌发培养基(B5盐3.1g/L、B5维他命、蔗糖20g/L、琼脂8g/L，pH5.6)中进行萌发，将种子接种于萌发培养基上，按以下条件培养：温度25±1℃；光周期(光/暗)为16/8h。萌发4-6天后取鲜绿的子叶节处膨大的大豆无菌苗，在子叶节下3-4mm处切去下胚轴，纵向切开子叶，去顶芽、侧芽和种子根。用解剖刀的刀背在子叶节处进行创伤，用农杆菌悬浮液接触创伤过的子叶节组织，其中农杆菌能够将所述SbHPPD-02核苷酸序列、SbHPPDm-F372A-02核苷酸序列、SbHPPDm-F383W-02核苷酸序列或SbHPPDm-F372A-F383W-02核苷酸序列传递至创伤过的子叶节组织(步骤1：侵染步骤)在此步骤中，子叶节组织优选地浸入农杆菌悬浮液(OD660＝0.5-0.8，侵染培养基(MS盐2.15g/L、B5维他命、蔗糖20g/L、葡萄糖10g/L、乙酰丁香酮(AS)40mg/L、2-吗啉乙磺酸(MES)4g/L、玉米素(ZT)2mg/L，pH5.3)中以启动接种。子叶节组织与农杆菌共培养一段时期(3天)(步骤2：共培养步骤)。优选地，子叶节组织在侵染步骤后在固体培养基(MS盐4.3g/L、B5维他命、蔗糖20g/L、葡萄糖10g/L、MES 4g/L、ZT 2mg/L、琼脂8g/L，pH5.6)上培养。在此共培养阶段后，可以有一个选择性的“恢复”步骤。在“恢复”步骤中，恢复培养基(B5盐3.1g/L、B5维他命、MES 1g/L、蔗糖30g/L、ZT 2mg/L、琼脂8g/L、头孢霉素150mg/L、谷氨酸100mg/L、天冬氨酸100mg/L，pH5.6)中至少存在一种己知抑制农杆菌生长的抗生素(头孢霉素150-250mg/L)，不添加植物转化体的选择剂(步骤3：恢复步骤)。优选地，子叶节再生的组织块在有抗生素但没有选择剂的固体培养基上培养，以消除农杆菌并为侵染细胞提供恢复期。接着，子叶节再生的组织块在含选择剂(草甘膦)的培养基上培养并选择生长着的转化愈伤组织(步骤4：选择步骤)。优选地，子叶节再生的组织块在有选择剂的筛选固体培养基(B5盐3.1g/L、B5维他命、MES1g/L、蔗糖30g/L、6-苄基腺嘌呤(6-BAP)1mg/L、琼脂8g/L、头孢霉素150mg/L、谷氨酸100mg/L、天冬氨酸100mg/L、N-(膦羧甲基)甘氨酸0.25mol/L，pH5.6)上培养，导致转化的细胞选择性生长。然后，转化的细胞再生成植物(步骤5：再生步骤)，优选地，在含选择剂的培养基上生长的子叶节再生的组织块在固体培养基(B5分化培养基和B5生根培养基)上培养以再生植物。
筛选得到的抗性组织块转移到所述B5分化培养基(B5盐3.1g/L、B5维他命、MES1g/L、蔗糖30g/L、ZT1mg/L、琼脂8g/L、头孢霉素150mg/L、谷氨酸50mg/L、天冬氨酸50mg/L、赤霉素1mg/L、生长素1mg/L、N-(膦羧甲基)甘氨酸0.25mol/L，pH5.6)上，25℃下培养分化。分化出来的小苗转移到所述B5生根培养基(B5盐3.1g/L、B5维他命、MES1g/L、蔗糖30g/L、琼脂8g/L、头孢霉素150mg/L、吲哚-3-丁酸(IBA)1mg/L)，在生根培养上，25℃下培养至约10cm高，移至温室培养至结实。在温室中，每天于26℃下培养16h，再于20℃下培养8h。
将上述转入SbHPPD-02核苷酸序列的大豆T0植株、转入SbHPPDm-F372A-02核苷酸序列的大豆T0植株、转入SbHPPDm-F383W-02核苷酸序列的大豆T0植株、转入SbHPPDm-F372A-F383W-02核苷酸序列的大豆T0植株和转入对照载体DBN11726N的大豆T0植株送入温室移栽进行培养、繁殖获得相应的转基因T1植株。
3、用TaqMan验证转基因大豆植株
分别取转入SbHPPD-02核苷酸序列的大豆T1植株、转入SbHPPDm-F372A-02核苷酸序列的大豆T1植株、转入SbHPPDm-F383W-02核苷酸序列的大豆T1植株、转入SbHPPDm-F372A-F383W-02核苷酸序列的大豆T1植株和转入对照载体DBN11726N的大豆T1植株的叶片约100mg作为样品，用Qiagen的DNeasy Plant Maxi Kit提取其基因组DNA，通过Taqman探针荧光定量PCR方法检测EPSPS基因拷贝数以确定突变的HPPD基因的拷贝数。同时以野生型大豆植株作为对照，按照上述方法进行检测分析。实验设3次重复，取平均值。
检测EPSPS基因拷贝数的具体方法如下：
步骤11、分别取转入SbHPPD-02核苷酸序列的大豆T1植株、转入SbHPPDm-F372A-02核苷酸序列的大豆T1植株、转入SbHPPDm-F383W-02核苷酸序列的大豆T1植株、转入SbHPPDm-F372A-F383W-02核苷酸序列的大豆T1植株、转入对照载体DBN11726N的大豆T1植株和野生型大豆植株的叶片各100mg，分别在研钵中用液氮研成匀浆，每个样品取3个重复；
步骤12、使用Qiagen的DNeasy Plant Mini Kit提取上述样品的基因组DNA，具体方法参考其产品说明书；
步骤13、用NanoDrop2000(Thermo Scientific)测定上述样品的基因组DNA浓度；
步骤14、调整上述样品的基因组DNA浓度至同一浓度值，所述浓度值的范围为80-100ng/μL；
步骤15、采用Taqman探针荧光定量PCR方法鉴定样品的拷贝数，以经过鉴定已知拷贝数的样品作为标准品，以野生型大豆植株的样品作为对照，每个样品3个重复，取其平均值；荧光定量PCR引物和探针序列分别是：
以下引物和探针用来检测EPSPS基因序列：
引物1：ctggaaggcgaggacgtcatcaata如序列表中SEQ ID NO：232所示；
引物2：tggcggcattgccgaaatcgag如序列表中SEQ ID NO：233所示；
探针1：atgcaggcgatgggcgcccgcatccgta如序列表中SEQ ID NO：234所示；
PCR反应体系为：
所述50×引物/探针混合物包含1mM浓度的每种引物各45μL，100μM浓度的探针50μL和860μL1×TE缓冲液，并且在4℃，贮藏在琥珀试管中。
PCR反应条件为：
利用SDS2.3软件(Applied Biosystems)分析数据。
通过分析EPSPS基因拷贝数的实验结果，进而证实SbHPPD-02核苷酸序列、SbHPPDm-F372A-02核苷酸序列、SbHPPDm-F383W-02核苷酸序列、SbHPPDm-F372A-F383W-02核苷酸序列和对照载体DBN11726N均己整合到所检测的大豆植株的染色体组中，而且转入SbHPPD-02核苷酸序列的大豆T1植株、转入SbHPPDm-F372A-02核苷酸序列的大豆T1植株、转入SbHPPDm-F383W-02核苷酸序列的大豆T1植株、转入SbHPPDm-F372A-F383W-02核苷酸序列的大豆T1植株和转入对照载体DBN11726N的大豆T1植株均获得了单拷贝的转基因大豆植株。
4、转基因大豆植株的对HPPD抑制剂除草剂耐受性效果检测
取转入SbHPPD-02核苷酸序列的大豆T1植株、转入SbHPPDm-F372A-02核苷酸序列的大豆T1植株、转入SbHPPDm-F383W-02核苷酸序列的大豆T1植株、转入SbHPPDm-F372A-F383W-02核苷酸序列的大豆T1植株、转入对照载体DBN11726N的大豆T1植株和野生型大豆植株(幼苗期V3-V4)，分别用3种浓度的苯吡唑草酮(25g ai/ha(1倍大田浓度，1×)、100g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))、3种浓度的异噁唑草酮(70g ai/ha(1倍大田浓度，1×)、280g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))和3种浓度的甲基磺草酮(105g ai/ha(1倍大田浓度，1×)、420g ai/ha(4倍大田浓度，4×)和0g ai/ha(水，0×))进行喷洒以检测大豆植株的除草剂耐受性。按照上述第一实施例6中的方法，在喷施7天(7DAT)后，统计每株植株受除草剂的损伤程度，并据此进行评分和抗性评价。转入SbHPPD-02核苷酸序列的大豆T1植株共2个株系(S1和S2)、转入SbHPPDm-F372A-02核苷酸序列的大豆T1植株共2个株系(S3和S4)、转入SbHPPDm-F383W-02核苷酸序列的大豆T1植株共2个株系(S5和S6)、转入SbHPPDm-F372A-F383W-02核苷酸序列的大豆T1植株共2个株系(S7和S8)，转入对照载体DBN11726N的大豆T1植株共1个株系(S9)，野生型大豆植株(CK1)共1个株系；从每个株系选8株进行测试。实验结果如表16至表18所示。
表16转基因大豆T1植株对苯吡唑草酮的耐受性实验结果
表17转基因大豆T1植株对异噁唑草酮的耐受性实验结果
表18转基因大豆T1植株对甲基磺草酮的耐受性实验结果
表16至表18表明：(1)与转入未突变HPPD基因的大豆植株和野生型大豆植株相比，SbHPPDm-F372A-02、SbHPPDm-F383W-02和SbHPPDm-F372A-F383W-02对不同浓度的HPPD抑制剂类除草剂产生了不同程度的耐受性，而DBN11726N对HPPD抑制剂类除草剂不具有耐受性；(2)转入372位点和383位点组合突变(F372A+F383W)的HPPD基因的大豆植株的除草剂耐受性不仅优于转入F372A或F383W单位点突变HPPD基因的大豆植株的除草剂耐受性，且进一步表现出协同增效的除草剂耐受性效果，表明突变的HPPD(F372A+F383W)能够赋予转基因大豆植物对HPPD抑制剂类除草剂协同增效的耐受性，也进一步证明了HPPD氨基酸序列的372位点和383位点组合突变赋予植物对HPPD抑制剂类除草剂耐受性的重要性和稳定性。
综上所述，本发明首次公开了对不同物种来源的羟基苯丙酮酸双加氧酶多肽的372位点+383位点进行组合突变可以赋予植物对吡唑啉酮类、异噁唑类和三酮类HPPD抑制剂类除草剂表现出协同增效的耐受性，特别是可以赋予转基因大豆植株耐受4倍大田浓度的苯吡唑草酮、异噁唑草酮和甲基磺草酮，因此在植物上应用前景广阔。
最后所应说明的是，以上实施例仅用以说明本发明的技术方案而非限制，尽管参照较佳实施例对本发明进行了详细说明，本领域的普通技术人员应当理解，可以对本发明的技术方案进行修改或者等同替换，而不脱离本发明技术方案的精神和范围。
序列表
<110> 北京大北农生物技术有限公司
<120> 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途
<130> FP1211361P
<160> 234
<170> SIPOSequenceListing 1.0
<210> 1
<211> 440
<212> PRT
<213> 燕麦野生型HPPD(AsHPPD的氨基酸序列Avena sativa)
<400> 1
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Phe Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 2
<211> 1323
<212> DNA
<213> AsHPPD-01核苷酸序列(Avena sativa)
<400> 2
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcttcacca agccagtagg ggacaggcca 1140
acgtttttcc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 3
<211> 1323
<212> DNA
<213> 人工序列-AsHPPD-02核苷酸序列(Artificial Sequence)
<400> 3
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attttcacta agccagtggg cgaccgccct 1140
acattcttcc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 4
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 4
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Phe Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 5
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 5
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgtttttcc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 6
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 6
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattcttcc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 7
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 7
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 8
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 8
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcttcacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 9
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 9
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attttcacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 10
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 10
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 11
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 11
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 12
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 12
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 13
<211> 542
<212> DNA
<213> 玄参花叶病毒的34S增强子(Figwort mosaic virus)
<400> 13
aattctcagt ccaaagcctc aacaaggtca gggtacagag tctccaaacc attagccaaa 60
agctacagga gatcaatgaa gaatcttcaa tcaaagtaaa ctactgttcc agcacatgca 120
tcatggtcag taagtttcag aaaaagacat ccaccgaaga cttaaagtta gtgggcatct 180
ttgaaagtaa tcttgtcaac atcgagcagc tggcttgtgg ggaccagaca aaaaaggaat 240
ggtgcagaat tgttaggcgc acctaccaaa agcatctttg cctttattgc aaagataaag 300
cagattcctc tagtacaagt ggggaacaaa ataacgtgga aaagagctgt cctgacagcc 360
cactcactaa tgcgtatgac gaacgcagtg acgaccacaa aagaattagc ttgagctcag 420
gatttagcag cattccagat tgggttcaat caacaaggta cgagccatat cactttattc 480
aaattggtat cgccaaaacc aagaaggaac tcccatcctc aaaggtttgt aaggaagaat 540
tc 542
<210> 14
<211> 1534
<212> DNA
<213> 油菜真核延长因子基因1α(Tsf1的启动子Brassica napus)
<400> 14
gattatgaca ttgctcgtgg aatgggacag ttatggtatt tttttgtaat aaattgtttc 60
cattgtcatg agattttgag gttaatctat gagacattga atcacttagc attagggatt 120
aagtagtcac aaatcgcatt caagaagctg aagaacacgt tatggtctaa tggttgtgtc 180
tctttattag aaaatgttgg tcagtagcta tatgcactgt ttctgtaaaa ccatgttggt 240
gttgtgttta tttcaagaca catgttgagt ccgttgattc agagcttttg tcttcgaaca 300
caatctagag agcaaatttg ggttcaattt ggatatcaat atgggttcga ttcagataga 360
acaataccct ttgatgtcgg gtttcgattt ggttgagatt catttttatc gggtttggtt 420
cgattttcga attcggttta ttcgccccct catagcatct acattctgca gattaatgta 480
caagttatgg aaaaaaaaat gtggttttcg aattcggttt agtagctaaa cgttgcttgc 540
agtgtagtta tgggaattat gaaacacgac cgaaggtatc aattagaaga acgggtcaac 600
gggtaagtat tgagaaatta ccggagggta aaaataaaca gtattctttt tttttcttaa 660
cgaccgacca aggttaaaaa aagaaaggag gacgagatac aggggcatga ctgtaattgt 720
acataagatc tgatctttaa accctaggtt tccttcgcat cagcaactat aaataattct 780
gagtgccact cttcttcatt cctagatctt tcgccttatc gctttagctg aggtaagcct 840
ttctatacgc atagacgctc tcttttctct tctctcgatc ttcgttgaaa cggtcctcga 900
tacgcatagg atcggttaga atcgttaatc tatcgtctta gatcttcttg attgttgaat 960
tgagcttcta ggatgtattg tatcatgtga tggatagttg attggatctc tttgagtgaa 1020
ctagctagct ttcgatgcgt gtgatttcag tataacagga tccgatgaat tatagctcgc 1080
ttacaattaa tctctgcaga tttattgttt aatcttggat ttgatgctcg ttgttgatag 1140
aggatcgttt atagaactta ttgattctgg aattgagctt gtgtgatgta ttgtatcatg 1200
tgatcgatag ctgatggatc tatttgagtg aactagcgta cgatcttaag atgagtgtgt 1260
attgtgaact gatgattcga gatcagcaaa acaagatctg atgatatctt cgtcttgtat 1320
gcatcttgaa tttcatgatt ttttattaat tatagctcgc ttagctcaaa ggatagagca 1380
ccacaaaatt ttattgtggt agaaatcggt tcgattccga tagcagctta ctgtgatgaa 1440
tgattttgag atttggtatt tgatatatgt ctactgtgtt gaatgatcgt ttatgcattg 1500
tttaatcgct gcagatttgc attgacaagt agcc 1534
<210> 15
<211> 228
<212> DNA
<213> 拟南芥叶绿体转运肽(Arabidopsis thaliana)
<400> 15
atggcgcaag ttagcagaat ctgcaatggt gtgcagaacc catctcttat ctccaatctc 60
tcgaaatcca gtcaacgcaa atctccctta tcggtttctc tgaagacgca gcagcatcca 120
cgagcttatc cgatttcgtc gtcgtgggga ttgaagaaga gtgggatgac gttaattggc 180
tctgagcttc gtcctcttaa ggtcatgtct tctgtttcca cggcgtgc 228
<210> 16
<211> 1368
<212> DNA
<213> 人工序列-5-烯醇丙酮酸莽草酸-3-磷酸合酶基因(Artificial Sequence)
<400> 16
atgcttcacg gtgcaagcag ccgtccagca actgctcgta agtcctctgg tctttctgga 60
accgtccgta ttccaggtga caagtctatc tcccacaggt ccttcatgtt tggaggtctc 120
gctagcggtg aaactcgtat caccggtctt ttggaaggtg aagatgttat caacactggt 180
aaggctatgc aagctatggg tgccagaatc cgtaaggaag gtgatacttg gatcattgat 240
ggtgttggta acggtggact ccttgctcct gaggctcctc tcgatttcgg taacgctgca 300
actggttgcc gtttgactat gggtcttgtt ggtgtttacg atttcgatag cactttcatt 360
ggtgacgctt ctctcactaa gcgtccaatg ggtcgtgtgt tgaacccact tcgcgaaatg 420
ggtgtgcagg tgaagtctga agacggtgat cgtcttccag ttaccttgcg tggaccaaag 480
actccaacgc caatcaccta cagggtacct atggcttccg ctcaagtgaa gtccgctgtt 540
ctgcttgctg gtctcaacac cccaggtatc accactgtta tcgagccaat catgactcgt 600
gaccacactg aaaagatgct tcaaggtttt ggtgctaacc ttaccgttga gactgatgct 660
gacggtgtgc gtaccatccg tcttgaaggt cgtggtaagc tcaccggtca agtgattgat 720
gttccaggtg atccatcctc tactgctttc ccattggttg ctgccttgct tgttccaggt 780
tccgacgtca ccatccttaa cgttttgatg aacccaaccc gtactggtct catcttgact 840
ctgcaggaaa tgggtgccga catcgaagtg atcaacccac gtcttgctgg tggagaagac 900
gtggctgact tgcgtgttcg ttcttctact ttgaagggtg ttactgttcc agaagaccgt 960
gctccttcta tgatcgacga gtatccaatt ctcgctgttg cagctgcatt cgctgaaggt 1020
gctaccgtta tgaacggttt ggaagaactc cgtgttaagg aaagcgaccg tctttctgct 1080
gtcgcaaacg gtctcaagct caacggtgtt gattgcgatg aaggtgagac ttctctcgtc 1140
gtgcgtggtc gtcctgacgg taagggtctc ggtaacgctt ctggagcagc tgtcgctacc 1200
cacctcgatc accgtatcgc tatgagcttc ctcgttatgg gtctcgtttc tgaaaaccct 1260
gttactgttg atgatgctac tatgatcgct actagcttcc cagagttcat ggatttgatg 1320
gctggtcttg gagctaagat cgaactctcc gacactaagg ctgcttga 1368
<210> 17
<211> 643
<212> DNA
<213> 豌豆RbcS基因的终止子(Pisum sativum)
<400> 17
agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat 60
tgcgcacaca ccagaatcct actgagtttg agtattatgg cattgggaaa actgtttttc 120
ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac 180
tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc 240
aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag 300
atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag 360
ttaatatgag gagtaaaaca cttgtagttg taccattatg cttattcact aggcaacaaa 420
tatattttca gacctagaaa agctgcaaat gttactgaat acaagtatgt cctcttgtgt 480
tttagacatt tatgaacttt cctttatgta attttccaga atccttgtca gattctaatc 540
attgctttat aattatagtt atactcatgg atttgtagtt gagtatgaaa atatttttta 600
atgcatttta tgacttgcca attgattgac aacatgcatc aat 643
<210> 18
<211> 1322
<212> DNA
<213> 拟南芥泛素(Ubiquitin10基因的启动子Arabidopsis thaliana)
<400> 18
gtcgacctgc aggtcaacgg atcaggatat tcttgtttaa gatgttgaac tctatggagg 60
tttgtatgaa ctgatgatct aggaccggat aagttccctt cttcatagcg aacttattca 120
aagaatgttt tgtgtatcat tcttgttaca ttgttattaa tgaaaaaata ttattggtca 180
ttggactgaa cacgagtgtt aaatatggac caggccccaa ataagatcca ttgatatatg 240
aattaaataa caagaataaa tcgagtcacc aaaccacttg ccttttttaa cgagacttgt 300
tcaccaactt gatacaaaag tcattatcct atgcaaatca ataatcatac aaaaatatcc 360
aataacacta aaaaattaaa agaaatggat aatttcacaa tatgttatac gataaagaag 420
ttacttttcc aagaaattca ctgattttat aagcccactt gcattagata aatggcaaaa 480
aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta gaaaatacga aatacgcttc 540
aatgcagtgg gacccacggt tcaattattg ccaattttca gctccaccgt atatttaaaa 600
aataaaacga taatgctaaa aaaatataaa tcgtaacgat cgttaaatct caacggctgg 660
atcttatgac gaccgttaga aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa 720
gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct 780
caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca 840
cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc 900
ctcgtctttt cttcttcttc ttctataaaa caatacccaa agcttcttct tcacaattca 960
gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt 1020
aaatttctgt gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt 1080
tcgtatatgt tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt 1140
tgatcgttag atatcatctt aattctcgat tagggtttca taaatatcat ccgatttgtt 1200
caaataattt gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg 1260
gtgttagttt ctagtttgtg cgatcgaatt tgtcgattaa tctgagtttt tctgattaac 1320
ag 1322
<210> 19
<211> 253
<212> DNA
<213> 胭脂碱合成酶基因的终止子(Agrobacterium tumefaciens)
<400> 19
gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 60
atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 120
atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 180
gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 240
atgttactag atc 253
<210> 20
<211> 530
<212> DNA
<213> 花椰菜花叶病毒35S启动子(Cauliflower mosaic virus)
<400> 20
ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac 60
agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg 120
agcacgacac gcttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg 180
caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag 240
ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc 300
attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg 360
gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc 420
aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt 480
cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca 530
<210> 21
<211> 552
<212> DNA
<213> 膦丝菌素N-乙酰基转移酶基因(Streptomyces viridochromogenes)
<400> 21
atgtctccgg agaggagacc agttgagatt aggccagcta cagcagctga tatggccgcg 60
gtttgtgata tcgttaacca ttacattgag acgtctacag tgaactttag gacagagcca 120
caaacaccac aagagtggat tgatgatcta gagaggttgc aagatagata cccttggttg 180
gttgctgagg ttgagggtgt tgtggctggt attgcttacg ctgggccctg gaaggctagg 240
aacgcttacg attggacagt tgagagtact gtttacgtgt cacataggca tcaaaggttg 300
ggcctaggat ccacattgta cacacatttg cttaagtcta tggaggcgca aggttttaag 360
tctgtggttg ctgttatagg ccttccaaac gatccatctg ttaggttgca tgaggctttg 420
ggatacacag cccggggtac attgcgcgca gctggataca agcatggtgg atggcatgat 480
gttggttttt ggcaaaggga ttttgagttg ccagctcctc caaggccagt taggccagtt 540
acccagatct ga 552
<210> 22
<211> 195
<212> DNA
<213> 花椰菜花叶病毒35S终止子(Cauliflower mosaic virus)
<400> 22
ctgaaatcac cagtctctct ctacaaatct atctctctct ataataatgt gtgagtagtt 60
cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa 120
cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa 180
accaaaatcc agtgg 195
<210> 23
<211> 445
<212> PRT
<213> 拟南芥野生型HPPD(AtHPPD的氨基酸序列Arabidopsis thaliana)
<400> 23
Met Gly His Gln Asn Ala Ala Val Ser Glu Asn Gln Asn His Asp Asp
1 5 10 15
Gly Ala Ala Ser Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe
20 25 30
Val Arg Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His
35 40 45
His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ala Arg Arg Phe
50 55 60
Ser Trp Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr
65 70 75 80
Gly Asn Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg
85 90 95
Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ile
100 105 110
Lys Pro Thr Thr Thr Ala Ser Ile Pro Ser Phe Asp His Gly Ser Cys
115 120 125
Arg Ser Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile
130 135 140
Glu Val Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly
145 150 155 160
Ala Ile Pro Ser Ser Pro Pro Ile Val Leu Asn Glu Ala Val Thr Ile
165 170 175
Ala Glu Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr
180 185 190
Lys Ala Glu Asp Thr Glu Lys Ser Glu Phe Leu Pro Gly Phe Glu Arg
195 200 205
Val Glu Asp Ala Ser Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu
210 215 220
Asp His Ala Val Gly Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr
225 230 235 240
Val Ala Gly Phe Thr Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp
245 250 255
Asp Val Gly Thr Ala Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Ser
260 265 270
Asn Asp Glu Met Val Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr
275 280 285
Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala
290 295 300
Gly Leu Gln His Leu Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu
305 310 315 320
Arg Glu Met Arg Lys Arg Ser Ser Ile Gly Gly Phe Asp Phe Met Pro
325 330 335
Ser Pro Pro Pro Thr Tyr Tyr Gln Asn Leu Lys Lys Arg Val Gly Asp
340 345 350
Val Leu Ser Asp Asp Gln Ile Lys Glu Cys Glu Glu Leu Gly Ile Leu
355 360 365
Val Asp Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro
370 375 380
Leu Gly Asp Arg Pro Thr Ile Phe Ile Glu Ile Ile Gln Arg Val Gly
385 390 395 400
Cys Met Met Lys Asp Glu Glu Gly Lys Ala Tyr Gln Ser Gly Gly Cys
405 410 415
Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu
420 425 430
Glu Tyr Glu Lys Thr Leu Glu Ala Lys Gln Leu Val Gly
435 440 445
<210> 24
<211> 1338
<212> DNA
<213> AtHPPD-01核苷酸序列(Arabidopsis thaliana)
<400> 24
atgggccacc aaaacgccgc cgtttcagag aatcaaaacc atgatgacgg cgctgcgtcg 60
tcgccgggat tcaagctcgt cggattttcc aagttcgtaa gaaagaatcc aaagtctgat 120
aaattcaagg ttaagcgctt ccatcacatc gagttctggt gcggcgacgc aaccaacgtc 180
gctcgtcgct tctcctgggg tctggggatg agattctccg ccaaatccga tctttccacc 240
ggaaacatgg ttcacgcctc ttacctactc acctccggtg acctccgatt ccttttcact 300
gctccttact ctccgtctct ctccgccgga gagattaaac cgacaaccac agcttctatc 360
ccaagtttcg atcacggctc ttgtcgttcc ttcttctctt cacatggtct cggtgttaga 420
gccgttgcga ttgaagtaga agacgcagag tcagctttct ccatcagtgt agctaatggc 480
gctattcctt cgtcgcctcc tatcgtcctc aatgaagcag ttacgatcgc tgaggttaaa 540
ctatacggcg atgttgttct ccgatatgtt agttacaaag cagaagatac cgaaaaatcc 600
gaattcttgc cagggttcga gcgtgtagag gatgcgtcgt cgttcccatt ggattatggt 660
atccggcggc ttgaccacgc cgtgggaaac gttcctgagc ttggtccggc tttaacttat 720
gtagcggggt tcactggttt tcaccaattc gcagagttca cagcagacga cgttggaacc 780
gccgagagcg gtttaaattc agcggtcctg gctagcaatg atgaaatggt tcttctaccg 840
attaacgagc cagtgcacgg aacaaagagg aagagtcaga ttcagacgta tttggaacat 900
aacgaaggcg cagggctaca acatctggct ctgatgagtg aagacatatt caggaccctg 960
agagagatga ggaagaggag cagtattgga ggattcgact tcatgccttc tcctccgcct 1020
acttactacc agaatctcaa gaaacgggtc ggcgacgtgc tcagcgatga tcagatcaag 1080
gagtgtgagg aattagggat tcttgtagac agagatgatc aagggacgtt gcttcaaatc 1140
ttcacaaaac cactaggtga caggccgacg atatttatag agataatcca gagagtagga 1200
tgcatgatga aagatgagga agggaaggct taccagagtg gaggatgtgg tggttttggc 1260
aaaggcaatt tctctgagct cttcaagtcc attgaagaat acgaaaagac tcttgaagcc 1320
aaacagttag tgggatga 1338
<210> 25
<211> 1338
<212> DNA
<213> 人工序列-AtHPPD-02核苷酸序列(Artificial Sequence)
<400> 25
atgggacatc agaatgctgc tgtatcagaa aatcaaaatc atgatgatgg agccgcctcc 60
tcaccagggt ttaagttggt tgggttctcc aagtttgtac ggaagaaccc aaaatccgac 120
aagttcaagg ttaaacgctt ccaccacatt gaattctggt gcggcgacgc caccaatgtt 180
gctcggcgtt tttcatgggg tttaggcatg agattctcag caaagagtga cctgagcacc 240
ggcaacatgg ttcatgctag ctatctctta acatctggag atttgaggtt tcttttcacg 300
gcgccgtact caccatcact ctccgccggc gagatcaaac ccaccacgac tgcaagcata 360
ccttcatttg atcatggtag ttgcagatcc ttcttctcgt cgcacggcct aggggtgcgc 420
gcggtcgcca tcgaagttga agatgcagaa tcagcgttta gtatcagcgt ggcaaatgga 480
gctattcctt cctcccctcc aattgtcctc aatgaggctg taactattgc cgaggtgaag 540
ttgtatggag atgttgtgct gagatatgtt tcttacaagg cagaggacac tgagaaatct 600
gaatttttgc ctggttttga aagggtcgag gatgcttcaa gttttcctct tgattacggc 660
atacgacggt tggatcacgc ggttggaaat gtaccagaac ttgggccggc gctcacttat 720
gtggctggtt tcactggatt ccatcaattt gctgagttca cagctgatga tgttgggact 780
gctgaaagtg ggttgaactc tgcagtcctc gcctctaatg atgaaatggt gctcctcccg 840
atcaacgagc ctgtgcatgg caccaaaagg aaatcacaaa tacagacata tcttgagcac 900
aatgaaggtg cagggcttca acatcttgca ctgatgtctg aagacatttt ccgtacgctg 960
agagagatga ggaagagaag tagtattggt ggatttgact tcatgccttc tcccccacca 1020
acttactacc agaacttgaa gaagagggtt ggtgatgtgc tatctgatga ccagatcaag 1080
gagtgtgaag aattgggcat tcttgttgat cgggacgacc aaggaacatt actgcagata 1140
tttacaaaac cacttggtga ccgccccact atttttattg agatcataca aagagtcggc 1200
tgcatgatga aggatgagga aggaaaagca tatcaatctg gtggttgtgg cggctttggg 1260
aaaggaaact tctctgagct gttcaaatcc atcgaggagt atgagaagac cttggaggcc 1320
aagcagctcg tgggttga 1338
<210> 26
<211> 445
<212> PRT
<213> 人工序列-AtHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 26
Met Gly His Gln Asn Ala Ala Val Ser Glu Asn Gln Asn His Asp Asp
1 5 10 15
Gly Ala Ala Ser Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe
20 25 30
Val Arg Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His
35 40 45
His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ala Arg Arg Phe
50 55 60
Ser Trp Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr
65 70 75 80
Gly Asn Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg
85 90 95
Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ile
100 105 110
Lys Pro Thr Thr Thr Ala Ser Ile Pro Ser Phe Asp His Gly Ser Cys
115 120 125
Arg Ser Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile
130 135 140
Glu Val Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly
145 150 155 160
Ala Ile Pro Ser Ser Pro Pro Ile Val Leu Asn Glu Ala Val Thr Ile
165 170 175
Ala Glu Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr
180 185 190
Lys Ala Glu Asp Thr Glu Lys Ser Glu Phe Leu Pro Gly Phe Glu Arg
195 200 205
Val Glu Asp Ala Ser Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu
210 215 220
Asp His Ala Val Gly Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr
225 230 235 240
Val Ala Gly Phe Thr Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp
245 250 255
Asp Val Gly Thr Ala Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Ser
260 265 270
Asn Asp Glu Met Val Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr
275 280 285
Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala
290 295 300
Gly Leu Gln His Leu Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu
305 310 315 320
Arg Glu Met Arg Lys Arg Ser Ser Ile Gly Gly Phe Asp Phe Met Pro
325 330 335
Ser Pro Pro Pro Thr Tyr Tyr Gln Asn Leu Lys Lys Arg Val Gly Asp
340 345 350
Val Leu Ser Asp Asp Gln Ile Lys Glu Cys Glu Glu Leu Gly Ile Leu
355 360 365
Val Asp Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro
370 375 380
Leu Gly Asp Arg Pro Thr Ile Phe Ile Glu Ile Ile Gln Arg Val Gly
385 390 395 400
Cys Met Met Lys Asp Glu Glu Gly Lys Ala Tyr Gln Ser Gly Gly Cys
405 410 415
Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu
420 425 430
Glu Tyr Glu Lys Thr Leu Glu Ala Lys Gln Leu Val Gly
435 440 445
<210> 27
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 27
atgggccacc aaaacgccgc cgtttcagag aatcaaaacc atgatgacgg cgctgcgtcg 60
tcgccgggat tcaagctcgt cggattttcc aagttcgtaa gaaagaatcc aaagtctgat 120
aaattcaagg ttaagcgctt ccatcacatc gagttctggt gcggcgacgc aaccaacgtc 180
gctcgtcgct tctcctgggg tctggggatg agattctccg ccaaatccga tctttccacc 240
ggaaacatgg ttcacgcctc ttacctactc acctccggtg acctccgatt ccttttcact 300
gctccttact ctccgtctct ctccgccgga gagattaaac cgacaaccac agcttctatc 360
ccaagtttcg atcacggctc ttgtcgttcc ttcttctctt cacatggtct cggtgttaga 420
gccgttgcga ttgaagtaga agacgcagag tcagctttct ccatcagtgt agctaatggc 480
gctattcctt cgtcgcctcc tatcgtcctc aatgaagcag ttacgatcgc tgaggttaaa 540
ctatacggcg atgttgttct ccgatatgtt agttacaaag cagaagatac cgaaaaatcc 600
gaattcttgc cagggttcga gcgtgtagag gatgcgtcgt cgttcccatt ggattatggt 660
atccggcggc ttgaccacgc cgtgggaaac gttcctgagc ttggtccggc tttaacttat 720
gtagcggggt tcactggttt tcaccaattc gcagagttca cagcagacga cgttggaacc 780
gccgagagcg gtttaaattc agcggtcctg gctagcaatg atgaaatggt tcttctaccg 840
attaacgagc cagtgcacgg aacaaagagg aagagtcaga ttcagacgta tttggaacat 900
aacgaaggcg cagggctaca acatctggct ctgatgagtg aagacatatt caggaccctg 960
agagagatga ggaagaggag cagtattgga ggattcgact tcatgccttc tcctccgcct 1020
acttactacc agaatctcaa gaaacgggtc ggcgacgtgc tcagcgatga tcagatcaag 1080
gagtgtgagg aattagggat tcttgtagac agagatgatc aagggacgtt gcttcaaatc 1140
gctacaaaac cactaggtga caggccgacg atatttatag agataatcca gagagtagga 1200
tgcatgatga aagatgagga agggaaggct taccagagtg gaggatgtgg tggttttggc 1260
aaaggcaatt tctctgagct cttcaagtcc attgaagaat acgaaaagac tcttgaagcc 1320
aaacagttag tgggatga 1338
<210> 28
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 28
atgggacatc agaatgctgc tgtatcagaa aatcaaaatc atgatgatgg agccgcctcc 60
tcaccagggt ttaagttggt tgggttctcc aagtttgtac ggaagaaccc aaaatccgac 120
aagttcaagg ttaaacgctt ccaccacatt gaattctggt gcggcgacgc caccaatgtt 180
gctcggcgtt tttcatgggg tttaggcatg agattctcag caaagagtga cctgagcacc 240
ggcaacatgg ttcatgctag ctatctctta acatctggag atttgaggtt tcttttcacg 300
gcgccgtact caccatcact ctccgccggc gagatcaaac ccaccacgac tgcaagcata 360
ccttcatttg atcatggtag ttgcagatcc ttcttctcgt cgcacggcct aggggtgcgc 420
gcggtcgcca tcgaagttga agatgcagaa tcagcgttta gtatcagcgt ggcaaatgga 480
gctattcctt cctcccctcc aattgtcctc aatgaggctg taactattgc cgaggtgaag 540
ttgtatggag atgttgtgct gagatatgtt tcttacaagg cagaggacac tgagaaatct 600
gaatttttgc ctggttttga aagggtcgag gatgcttcaa gttttcctct tgattacggc 660
atacgacggt tggatcacgc ggttggaaat gtaccagaac ttgggccggc gctcacttat 720
gtggctggtt tcactggatt ccatcaattt gctgagttca cagctgatga tgttgggact 780
gctgaaagtg ggttgaactc tgcagtcctc gcctctaatg atgaaatggt gctcctcccg 840
atcaacgagc ctgtgcatgg caccaaaagg aaatcacaaa tacagacata tcttgagcac 900
aatgaaggtg cagggcttca acatcttgca ctgatgtctg aagacatttt ccgtacgctg 960
agagagatga ggaagagaag tagtattggt ggatttgact tcatgccttc tcccccacca 1020
acttactacc agaacttgaa gaagagggtt ggtgatgtgc tatctgatga ccagatcaag 1080
gagtgtgaag aattgggcat tcttgttgat cgggacgacc aaggaacatt actgcagata 1140
gccacaaaac cacttggtga ccgccccact atttttattg agatcataca aagagtcggc 1200
tgcatgatga aggatgagga aggaaaagca tatcaatctg gtggttgtgg cggctttggg 1260
aaaggaaact tctctgagct gttcaaatcc atcgaggagt atgagaagac cttggaggcc 1320
aagcagctcg tgggttga 1338
<210> 29
<211> 445
<212> PRT
<213> 人工序列-AtHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 29
Met Gly His Gln Asn Ala Ala Val Ser Glu Asn Gln Asn His Asp Asp
1 5 10 15
Gly Ala Ala Ser Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe
20 25 30
Val Arg Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His
35 40 45
His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ala Arg Arg Phe
50 55 60
Ser Trp Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr
65 70 75 80
Gly Asn Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg
85 90 95
Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ile
100 105 110
Lys Pro Thr Thr Thr Ala Ser Ile Pro Ser Phe Asp His Gly Ser Cys
115 120 125
Arg Ser Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile
130 135 140
Glu Val Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly
145 150 155 160
Ala Ile Pro Ser Ser Pro Pro Ile Val Leu Asn Glu Ala Val Thr Ile
165 170 175
Ala Glu Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr
180 185 190
Lys Ala Glu Asp Thr Glu Lys Ser Glu Phe Leu Pro Gly Phe Glu Arg
195 200 205
Val Glu Asp Ala Ser Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu
210 215 220
Asp His Ala Val Gly Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr
225 230 235 240
Val Ala Gly Phe Thr Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp
245 250 255
Asp Val Gly Thr Ala Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Ser
260 265 270
Asn Asp Glu Met Val Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr
275 280 285
Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala
290 295 300
Gly Leu Gln His Leu Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu
305 310 315 320
Arg Glu Met Arg Lys Arg Ser Ser Ile Gly Gly Phe Asp Phe Met Pro
325 330 335
Ser Pro Pro Pro Thr Tyr Tyr Gln Asn Leu Lys Lys Arg Val Gly Asp
340 345 350
Val Leu Ser Asp Asp Gln Ile Lys Glu Cys Glu Glu Leu Gly Ile Leu
355 360 365
Val Asp Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro
370 375 380
Leu Gly Asp Arg Pro Thr Ile Trp Ile Glu Ile Ile Gln Arg Val Gly
385 390 395 400
Cys Met Met Lys Asp Glu Glu Gly Lys Ala Tyr Gln Ser Gly Gly Cys
405 410 415
Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu
420 425 430
Glu Tyr Glu Lys Thr Leu Glu Ala Lys Gln Leu Val Gly
435 440 445
<210> 30
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 30
atgggccacc aaaacgccgc cgtttcagag aatcaaaacc atgatgacgg cgctgcgtcg 60
tcgccgggat tcaagctcgt cggattttcc aagttcgtaa gaaagaatcc aaagtctgat 120
aaattcaagg ttaagcgctt ccatcacatc gagttctggt gcggcgacgc aaccaacgtc 180
gctcgtcgct tctcctgggg tctggggatg agattctccg ccaaatccga tctttccacc 240
ggaaacatgg ttcacgcctc ttacctactc acctccggtg acctccgatt ccttttcact 300
gctccttact ctccgtctct ctccgccgga gagattaaac cgacaaccac agcttctatc 360
ccaagtttcg atcacggctc ttgtcgttcc ttcttctctt cacatggtct cggtgttaga 420
gccgttgcga ttgaagtaga agacgcagag tcagctttct ccatcagtgt agctaatggc 480
gctattcctt cgtcgcctcc tatcgtcctc aatgaagcag ttacgatcgc tgaggttaaa 540
ctatacggcg atgttgttct ccgatatgtt agttacaaag cagaagatac cgaaaaatcc 600
gaattcttgc cagggttcga gcgtgtagag gatgcgtcgt cgttcccatt ggattatggt 660
atccggcggc ttgaccacgc cgtgggaaac gttcctgagc ttggtccggc tttaacttat 720
gtagcggggt tcactggttt tcaccaattc gcagagttca cagcagacga cgttggaacc 780
gccgagagcg gtttaaattc agcggtcctg gctagcaatg atgaaatggt tcttctaccg 840
attaacgagc cagtgcacgg aacaaagagg aagagtcaga ttcagacgta tttggaacat 900
aacgaaggcg cagggctaca acatctggct ctgatgagtg aagacatatt caggaccctg 960
agagagatga ggaagaggag cagtattgga ggattcgact tcatgccttc tcctccgcct 1020
acttactacc agaatctcaa gaaacgggtc ggcgacgtgc tcagcgatga tcagatcaag 1080
gagtgtgagg aattagggat tcttgtagac agagatgatc aagggacgtt gcttcaaatc 1140
ttcacaaaac cactaggtga caggccgacg atatggatag agataatcca gagagtagga 1200
tgcatgatga aagatgagga agggaaggct taccagagtg gaggatgtgg tggttttggc 1260
aaaggcaatt tctctgagct cttcaagtcc attgaagaat acgaaaagac tcttgaagcc 1320
aaacagttag tgggatga 1338
<210> 31
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 31
atgggacatc agaatgctgc tgtatcagaa aatcaaaatc atgatgatgg agccgcctcc 60
tcaccagggt ttaagttggt tgggttctcc aagtttgtac ggaagaaccc aaaatccgac 120
aagttcaagg ttaaacgctt ccaccacatt gaattctggt gcggcgacgc caccaatgtt 180
gctcggcgtt tttcatgggg tttaggcatg agattctcag caaagagtga cctgagcacc 240
ggcaacatgg ttcatgctag ctatctctta acatctggag atttgaggtt tcttttcacg 300
gcgccgtact caccatcact ctccgccggc gagatcaaac ccaccacgac tgcaagcata 360
ccttcatttg atcatggtag ttgcagatcc ttcttctcgt cgcacggcct aggggtgcgc 420
gcggtcgcca tcgaagttga agatgcagaa tcagcgttta gtatcagcgt ggcaaatgga 480
gctattcctt cctcccctcc aattgtcctc aatgaggctg taactattgc cgaggtgaag 540
ttgtatggag atgttgtgct gagatatgtt tcttacaagg cagaggacac tgagaaatct 600
gaatttttgc ctggttttga aagggtcgag gatgcttcaa gttttcctct tgattacggc 660
atacgacggt tggatcacgc ggttggaaat gtaccagaac ttgggccggc gctcacttat 720
gtggctggtt tcactggatt ccatcaattt gctgagttca cagctgatga tgttgggact 780
gctgaaagtg ggttgaactc tgcagtcctc gcctctaatg atgaaatggt gctcctcccg 840
atcaacgagc ctgtgcatgg caccaaaagg aaatcacaaa tacagacata tcttgagcac 900
aatgaaggtg cagggcttca acatcttgca ctgatgtctg aagacatttt ccgtacgctg 960
agagagatga ggaagagaag tagtattggt ggatttgact tcatgccttc tcccccacca 1020
acttactacc agaacttgaa gaagagggtt ggtgatgtgc tatctgatga ccagatcaag 1080
gagtgtgaag aattgggcat tcttgttgat cgggacgacc aaggaacatt actgcagata 1140
tttacaaaac cacttggtga ccgccccact atttggattg agatcataca aagagtcggc 1200
tgcatgatga aggatgagga aggaaaagca tatcaatctg gtggttgtgg cggctttggg 1260
aaaggaaact tctctgagct gttcaaatcc atcgaggagt atgagaagac cttggaggcc 1320
aagcagctcg tgggttga 1338
<210> 32
<211> 445
<212> PRT
<213> 人工序列-AtHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 32
Met Gly His Gln Asn Ala Ala Val Ser Glu Asn Gln Asn His Asp Asp
1 5 10 15
Gly Ala Ala Ser Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe
20 25 30
Val Arg Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His
35 40 45
His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ala Arg Arg Phe
50 55 60
Ser Trp Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr
65 70 75 80
Gly Asn Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg
85 90 95
Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ile
100 105 110
Lys Pro Thr Thr Thr Ala Ser Ile Pro Ser Phe Asp His Gly Ser Cys
115 120 125
Arg Ser Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile
130 135 140
Glu Val Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly
145 150 155 160
Ala Ile Pro Ser Ser Pro Pro Ile Val Leu Asn Glu Ala Val Thr Ile
165 170 175
Ala Glu Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr
180 185 190
Lys Ala Glu Asp Thr Glu Lys Ser Glu Phe Leu Pro Gly Phe Glu Arg
195 200 205
Val Glu Asp Ala Ser Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu
210 215 220
Asp His Ala Val Gly Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr
225 230 235 240
Val Ala Gly Phe Thr Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp
245 250 255
Asp Val Gly Thr Ala Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Ser
260 265 270
Asn Asp Glu Met Val Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr
275 280 285
Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala
290 295 300
Gly Leu Gln His Leu Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu
305 310 315 320
Arg Glu Met Arg Lys Arg Ser Ser Ile Gly Gly Phe Asp Phe Met Pro
325 330 335
Ser Pro Pro Pro Thr Tyr Tyr Gln Asn Leu Lys Lys Arg Val Gly Asp
340 345 350
Val Leu Ser Asp Asp Gln Ile Lys Glu Cys Glu Glu Leu Gly Ile Leu
355 360 365
Val Asp Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro
370 375 380
Leu Gly Asp Arg Pro Thr Ile Trp Ile Glu Ile Ile Gln Arg Val Gly
385 390 395 400
Cys Met Met Lys Asp Glu Glu Gly Lys Ala Tyr Gln Ser Gly Gly Cys
405 410 415
Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu
420 425 430
Glu Tyr Glu Lys Thr Leu Glu Ala Lys Gln Leu Val Gly
435 440 445
<210> 33
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 33
atgggccacc aaaacgccgc cgtttcagag aatcaaaacc atgatgacgg cgctgcgtcg 60
tcgccgggat tcaagctcgt cggattttcc aagttcgtaa gaaagaatcc aaagtctgat 120
aaattcaagg ttaagcgctt ccatcacatc gagttctggt gcggcgacgc aaccaacgtc 180
gctcgtcgct tctcctgggg tctggggatg agattctccg ccaaatccga tctttccacc 240
ggaaacatgg ttcacgcctc ttacctactc acctccggtg acctccgatt ccttttcact 300
gctccttact ctccgtctct ctccgccgga gagattaaac cgacaaccac agcttctatc 360
ccaagtttcg atcacggctc ttgtcgttcc ttcttctctt cacatggtct cggtgttaga 420
gccgttgcga ttgaagtaga agacgcagag tcagctttct ccatcagtgt agctaatggc 480
gctattcctt cgtcgcctcc tatcgtcctc aatgaagcag ttacgatcgc tgaggttaaa 540
ctatacggcg atgttgttct ccgatatgtt agttacaaag cagaagatac cgaaaaatcc 600
gaattcttgc cagggttcga gcgtgtagag gatgcgtcgt cgttcccatt ggattatggt 660
atccggcggc ttgaccacgc cgtgggaaac gttcctgagc ttggtccggc tttaacttat 720
gtagcggggt tcactggttt tcaccaattc gcagagttca cagcagacga cgttggaacc 780
gccgagagcg gtttaaattc agcggtcctg gctagcaatg atgaaatggt tcttctaccg 840
attaacgagc cagtgcacgg aacaaagagg aagagtcaga ttcagacgta tttggaacat 900
aacgaaggcg cagggctaca acatctggct ctgatgagtg aagacatatt caggaccctg 960
agagagatga ggaagaggag cagtattgga ggattcgact tcatgccttc tcctccgcct 1020
acttactacc agaatctcaa gaaacgggtc ggcgacgtgc tcagcgatga tcagatcaag 1080
gagtgtgagg aattagggat tcttgtagac agagatgatc aagggacgtt gcttcaaatc 1140
gctacaaaac cactaggtga caggccgacg atatggatag agataatcca gagagtagga 1200
tgcatgatga aagatgagga agggaaggct taccagagtg gaggatgtgg tggttttggc 1260
aaaggcaatt tctctgagct cttcaagtcc attgaagaat acgaaaagac tcttgaagcc 1320
aaacagttag tgggatga 1338
<210> 34
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 34
atgggacatc agaatgctgc tgtatcagaa aatcaaaatc atgatgatgg agccgcctcc 60
tcaccagggt ttaagttggt tgggttctcc aagtttgtac ggaagaaccc aaaatccgac 120
aagttcaagg ttaaacgctt ccaccacatt gaattctggt gcggcgacgc caccaatgtt 180
gctcggcgtt tttcatgggg tttaggcatg agattctcag caaagagtga cctgagcacc 240
ggcaacatgg ttcatgctag ctatctctta acatctggag atttgaggtt tcttttcacg 300
gcgccgtact caccatcact ctccgccggc gagatcaaac ccaccacgac tgcaagcata 360
ccttcatttg atcatggtag ttgcagatcc ttcttctcgt cgcacggcct aggggtgcgc 420
gcggtcgcca tcgaagttga agatgcagaa tcagcgttta gtatcagcgt ggcaaatgga 480
gctattcctt cctcccctcc aattgtcctc aatgaggctg taactattgc cgaggtgaag 540
ttgtatggag atgttgtgct gagatatgtt tcttacaagg cagaggacac tgagaaatct 600
gaatttttgc ctggttttga aagggtcgag gatgcttcaa gttttcctct tgattacggc 660
atacgacggt tggatcacgc ggttggaaat gtaccagaac ttgggccggc gctcacttat 720
gtggctggtt tcactggatt ccatcaattt gctgagttca cagctgatga tgttgggact 780
gctgaaagtg ggttgaactc tgcagtcctc gcctctaatg atgaaatggt gctcctcccg 840
atcaacgagc ctgtgcatgg caccaaaagg aaatcacaaa tacagacata tcttgagcac 900
aatgaaggtg cagggcttca acatcttgca ctgatgtctg aagacatttt ccgtacgctg 960
agagagatga ggaagagaag tagtattggt ggatttgact tcatgccttc tcccccacca 1020
acttactacc agaacttgaa gaagagggtt ggtgatgtgc tatctgatga ccagatcaag 1080
gagtgtgaag aattgggcat tcttgttgat cgggacgacc aaggaacatt actgcagata 1140
gccacaaaac cacttggtga ccgccccact atttggattg agatcataca aagagtcggc 1200
tgcatgatga aggatgagga aggaaaagca tatcaatctg gtggttgtgg cggctttggg 1260
aaaggaaact tctctgagct gttcaaatcc atcgaggagt atgagaagac cttggaggcc 1320
aagcagctcg tgggttga 1338
<210> 35
<211> 435
<212> PRT
<213> 苜蓿野生型HPPD(MsHPPD的氨基酸序列Medicago Sativa Linn)
<400> 35
Met Ala Ile Glu Thr Glu Thr Gln Thr Gln Thr Gly Phe Lys Leu Val
1 5 10 15
Gly Phe Lys Asn Phe Val Arg Ala Asn Pro Lys Ser Asp Arg Phe Lys
20 25 30
Val Lys Arg Phe His His Val Glu Phe Trp Cys Thr Asp Ala Thr Asn
35 40 45
Thr Ala Arg Arg Phe Ser His Gly Leu Gly Met Pro Ile Val Ala Lys
50 55 60
Ser Asp Leu Ser Thr Gly Asn Gln Thr His Ala Ser Tyr Leu Leu Arg
65 70 75 80
Ser Gly Asp Leu Asn Phe Leu Phe Ser Ala Ala Tyr Ser Pro Ser Ile
85 90 95
Ser Leu Ser Ser Pro Ser Ser Thr Ala Ala Ile Pro Thr Phe Ser Ala
100 105 110
Ser Asn Cys Phe Ser Phe Ser Ala Ser His Gly Leu Ala Val Arg Ala
115 120 125
Val Ala Val Glu Val Glu Asp Ala Glu Leu Ala Phe Thr Thr Ser Val
130 135 140
Asn Asn Gly Ala Ile Pro Ser Ser Pro Pro Val Ile Leu Glu Asn His
145 150 155 160
Val Lys Leu Ala Glu Val Arg Leu Tyr Gly Asp Val Val Leu Arg Tyr
165 170 175
Val Ser Tyr Asn Asp Pro Asn Pro Asn Gln Asn Pro Asn Leu Leu Phe
180 185 190
Leu Pro Gly Phe Glu Arg Val Ser Asp Glu Ser Ser Asn Ser Ser Leu
195 200 205
Asp Phe Gly Ile Arg Arg Leu Asp His Ala Val Gly Asn Val Pro Glu
210 215 220
Leu Ser Ser Ala Val Lys Tyr Val Lys Asp Phe Thr Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Ser Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Glu Glu Thr Val Leu Leu Pro Met
260 265 270
Asn Glu Pro Val Tyr Gly Thr Lys Arg Lys Ser Gln Ile Glu Thr Tyr
275 280 285
Leu Glu His Asn Glu Gly Ala Gly Leu Gln His Leu Ala Leu Lys Ser
290 295 300
Glu Asp Ile Phe Arg Thr Leu Arg Glu Met Arg Lys Arg Ser Gly Val
305 310 315 320
Gly Gly Phe Glu Phe Met Pro Ser Pro Pro Val Thr Tyr Tyr Arg Asn
325 330 335
Leu Lys Asn Arg Val Gly Asp Val Leu Ser Asp Glu Gln Ile Lys Glu
340 345 350
Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp Gln Gly Thr Leu
355 360 365
Leu Gln Ile Phe Thr Lys Pro Ile Gly Asp Arg Pro Thr Ile Phe Ile
370 375 380
Glu Ile Ile Gln Arg Val Gly Cys Met Leu Lys Asp Glu Glu Gly Lys
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr Leu Glu Thr Arg
420 425 430
Arg Thr Ala
435
<210> 36
<211> 1308
<212> DNA
<213> MsHPPD-01核苷酸序列(Medicago Sativa Linn)
<400> 36
atggccatcg aaacagaaac tcaaacccaa accgggttca aactagttgg gttcaagaac 60
ttcgtccgag ccaatccaaa atcagaccgg ttcaaagtaa aacgcttcca ccatgttgag 120
ttctggtgca ccgacgcaac aaacaccgca cgccgtttct ctcacggact cggaatgccg 180
attgtcgcta aatcagacct ctccaccgga aatcaaactc acgcctccta tctcctccgc 240
tccggtgacc tcaacttcct cttctccgcc gcttattccc cttccatttc tctctcctca 300
ccttcctcaa ctgccgcaat ccccactttc tccgcatcca attgtttctc cttctccgct 360
tcccacggcc tcgccgtccg tgctgttgct gttgaagtcg aagacgccga actggctttc 420
accaccagcg taaacaacgg tgccattcca tcgtctcctc cagttatcct cgaaaaccac 480
gtcaaactgg ccgaagttcg tctctacggc gacgttgttc tacgttacgt cagttacaac 540
gacccgaacc caaaccagaa tccgaacctg ctcttcctcc ctggttttga acgtgtatca 600
gatgaatcat ctaattcatc gttagatttc ggaatccggc gactagacca tgccgttgga 660
aatgtaccgg agctttcatc cgctgtgaaa tacgtgaaag atttcaccgg atttcatgaa 720
tttgctgagt ttacagcgga agacgttgga acgagtgaga gtggattaaa ctcagtggtg 780
ttagctaaca atgaagaaac agtgttgctt ccgatgaatg aaccggttta tggaacaaag 840
aggaagagtc aaatagaaac gtatttggaa cataatgaag gtgctgggtt acaacatttg 900
gcattgaagt ctgaggatat atttaggaca ttgagggaaa tgagaaagag aagtggtgtt 960
ggtgggtttg agtttatgcc ttctcctccg gtcacttatt atcgtaattt gaagaatcgc 1020
gttggcgatg ttttgagtga tgaacagatt aaggagtgtg aagagcttgg gattttggtt 1080
gatagagatg atcagggtac tctgcttcag atttttacca agcctattgg agataggcca 1140
accatattta tagagataat tcagagagtt ggatgcatgc tcaaggacga agaagggaag 1200
gagtatcaaa agggaggatg tgggggcttt ggaaaaggaa acttctcgga gcttttcaaa 1260
tccattgaag agtatgagaa gactttggaa actagaagaa ctgcatga 1308
<210> 37
<211> 1308
<212> DNA
<213> 人工序列-MsHPPD-02核苷酸序列(Artificial Sequence)
<400> 37
atggctattg aaaccgaaac acaaactcag actggattta aactggtagg gttcaagaat 60
ttcgttaggg ctaatcccaa gtctgatcgt tttaaggtca agaggttcca ccacgtggag 120
ttctggtgca ctgatgcaac caacacggcg cggcgttttt cacatggtct tggaatgcct 180
attgttgcaa aatccgacct ctccacgggg aaccaaacac acgcctctta cctgctacgg 240
agtggtgact tgaacttcct cttcagcgcc gcctactcac cctccatatc gttgtcttca 300
ccatcctcga ccgcagcaat tcctacattt tctgcgagca actgcttctc tttctctgcc 360
agtcatggcc tcgccgtcag agcagtggcc gttgaagtag aggatgctga acttgctttt 420
acaacttcag tgaataatgg agcaataccg agcagtccgc cggtcatact tgagaatcat 480
gtgaagctag cggaggtgag gctttatggg gacgtcgttt taagatatgt cagctacaat 540
gatccaaacc ccaatcaaaa tccaaatttg ttgttccttc ctggcttcga gcgtgttagc 600
gatgaatctt caaactcttc actggatttt ggaatcagac ggttggatca tgctgttgga 660
aatgttcctg aactctcgag cgctgtgaaa tatgtgaagg acttcaccgg ttttcatgaa 720
tttgctgagt ttactgcaga agatgtgggt acatcagaga gtggcttgaa ttccgtggtg 780
ctcgccaaca acgaggagac tgtactctta ccaatgaatg agccagtcta tggcaccaag 840
cgtaaatctc aaattgagac atatttggag cacaatgaag gagctggact tcagcatctc 900
gcgctgaaga gtgaggacat cttccgcacc ctacgggaga tgaggaaaag aagtggtgtt 960
ggtgggtttg aattcatgcc atcacctcct gtcacctact acaggaacct gaagaacaga 1020
gttggtgatg tactttcgga cgagcagata aaggaatgtg aagagttggg gattttggtt 1080
gatagagatg atcaaggaac actcctgcag atttttacaa aaccaattgg cgaccgcccc 1140
actatcttca tagagatcat ccagagggtg gggtgcatgc tcaaagacga ggaagggaag 1200
gagtaccaga agggcggctg tggtggtttt gggaaaggaa acttttctga gttattcaaa 1260
tcaattgaag agtatgagaa gacgcttgaa acgcgtcgca ctgcttga 1308
<210> 38
<211> 435
<212> PRT
<213> 人工序列-MsHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 38
Met Ala Ile Glu Thr Glu Thr Gln Thr Gln Thr Gly Phe Lys Leu Val
1 5 10 15
Gly Phe Lys Asn Phe Val Arg Ala Asn Pro Lys Ser Asp Arg Phe Lys
20 25 30
Val Lys Arg Phe His His Val Glu Phe Trp Cys Thr Asp Ala Thr Asn
35 40 45
Thr Ala Arg Arg Phe Ser His Gly Leu Gly Met Pro Ile Val Ala Lys
50 55 60
Ser Asp Leu Ser Thr Gly Asn Gln Thr His Ala Ser Tyr Leu Leu Arg
65 70 75 80
Ser Gly Asp Leu Asn Phe Leu Phe Ser Ala Ala Tyr Ser Pro Ser Ile
85 90 95
Ser Leu Ser Ser Pro Ser Ser Thr Ala Ala Ile Pro Thr Phe Ser Ala
100 105 110
Ser Asn Cys Phe Ser Phe Ser Ala Ser His Gly Leu Ala Val Arg Ala
115 120 125
Val Ala Val Glu Val Glu Asp Ala Glu Leu Ala Phe Thr Thr Ser Val
130 135 140
Asn Asn Gly Ala Ile Pro Ser Ser Pro Pro Val Ile Leu Glu Asn His
145 150 155 160
Val Lys Leu Ala Glu Val Arg Leu Tyr Gly Asp Val Val Leu Arg Tyr
165 170 175
Val Ser Tyr Asn Asp Pro Asn Pro Asn Gln Asn Pro Asn Leu Leu Phe
180 185 190
Leu Pro Gly Phe Glu Arg Val Ser Asp Glu Ser Ser Asn Ser Ser Leu
195 200 205
Asp Phe Gly Ile Arg Arg Leu Asp His Ala Val Gly Asn Val Pro Glu
210 215 220
Leu Ser Ser Ala Val Lys Tyr Val Lys Asp Phe Thr Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Ser Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Glu Glu Thr Val Leu Leu Pro Met
260 265 270
Asn Glu Pro Val Tyr Gly Thr Lys Arg Lys Ser Gln Ile Glu Thr Tyr
275 280 285
Leu Glu His Asn Glu Gly Ala Gly Leu Gln His Leu Ala Leu Lys Ser
290 295 300
Glu Asp Ile Phe Arg Thr Leu Arg Glu Met Arg Lys Arg Ser Gly Val
305 310 315 320
Gly Gly Phe Glu Phe Met Pro Ser Pro Pro Val Thr Tyr Tyr Arg Asn
325 330 335
Leu Lys Asn Arg Val Gly Asp Val Leu Ser Asp Glu Gln Ile Lys Glu
340 345 350
Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp Gln Gly Thr Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Ile Gly Asp Arg Pro Thr Ile Phe Ile
370 375 380
Glu Ile Ile Gln Arg Val Gly Cys Met Leu Lys Asp Glu Glu Gly Lys
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr Leu Glu Thr Arg
420 425 430
Arg Thr Ala
435
<210> 39
<211> 1308
<212> DNA
<213> 人工序列-MsHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 39
atggccatcg aaacagaaac tcaaacccaa accgggttca aactagttgg gttcaagaac 60
ttcgtccgag ccaatccaaa atcagaccgg ttcaaagtaa aacgcttcca ccatgttgag 120
ttctggtgca ccgacgcaac aaacaccgca cgccgtttct ctcacggact cggaatgccg 180
attgtcgcta aatcagacct ctccaccgga aatcaaactc acgcctccta tctcctccgc 240
tccggtgacc tcaacttcct cttctccgcc gcttattccc cttccatttc tctctcctca 300
ccttcctcaa ctgccgcaat ccccactttc tccgcatcca attgtttctc cttctccgct 360
tcccacggcc tcgccgtccg tgctgttgct gttgaagtcg aagacgccga actggctttc 420
accaccagcg taaacaacgg tgccattcca tcgtctcctc cagttatcct cgaaaaccac 480
gtcaaactgg ccgaagttcg tctctacggc gacgttgttc tacgttacgt cagttacaac 540
gacccgaacc caaaccagaa tccgaacctg ctcttcctcc ctggttttga acgtgtatca 600
gatgaatcat ctaattcatc gttagatttc ggaatccggc gactagacca tgccgttgga 660
aatgtaccgg agctttcatc cgctgtgaaa tacgtgaaag atttcaccgg atttcatgaa 720
tttgctgagt ttacagcgga agacgttgga acgagtgaga gtggattaaa ctcagtggtg 780
ttagctaaca atgaagaaac agtgttgctt ccgatgaatg aaccggttta tggaacaaag 840
aggaagagtc aaatagaaac gtatttggaa cataatgaag gtgctgggtt acaacatttg 900
gcattgaagt ctgaggatat atttaggaca ttgagggaaa tgagaaagag aagtggtgtt 960
ggtgggtttg agtttatgcc ttctcctccg gtcacttatt atcgtaattt gaagaatcgc 1020
gttggcgatg ttttgagtga tgaacagatt aaggagtgtg aagagcttgg gattttggtt 1080
gatagagatg atcagggtac tctgcttcag attgctacca agcctattgg agataggcca 1140
accatattta tagagataat tcagagagtt ggatgcatgc tcaaggacga agaagggaag 1200
gagtatcaaa agggaggatg tgggggcttt ggaaaaggaa acttctcgga gcttttcaaa 1260
tccattgaag agtatgagaa gactttggaa actagaagaa ctgcatga 1308
<210> 40
<211> 1308
<212> DNA
<213> 人工序列-MsHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 40
atggctattg aaaccgaaac acaaactcag actggattta aactggtagg gttcaagaat 60
ttcgttaggg ctaatcccaa gtctgatcgt tttaaggtca agaggttcca ccacgtggag 120
ttctggtgca ctgatgcaac caacacggcg cggcgttttt cacatggtct tggaatgcct 180
attgttgcaa aatccgacct ctccacgggg aaccaaacac acgcctctta cctgctacgg 240
agtggtgact tgaacttcct cttcagcgcc gcctactcac cctccatatc gttgtcttca 300
ccatcctcga ccgcagcaat tcctacattt tctgcgagca actgcttctc tttctctgcc 360
agtcatggcc tcgccgtcag agcagtggcc gttgaagtag aggatgctga acttgctttt 420
acaacttcag tgaataatgg agcaataccg agcagtccgc cggtcatact tgagaatcat 480
gtgaagctag cggaggtgag gctttatggg gacgtcgttt taagatatgt cagctacaat 540
gatccaaacc ccaatcaaaa tccaaatttg ttgttccttc ctggcttcga gcgtgttagc 600
gatgaatctt caaactcttc actggatttt ggaatcagac ggttggatca tgctgttgga 660
aatgttcctg aactctcgag cgctgtgaaa tatgtgaagg acttcaccgg ttttcatgaa 720
tttgctgagt ttactgcaga agatgtgggt acatcagaga gtggcttgaa ttccgtggtg 780
ctcgccaaca acgaggagac tgtactctta ccaatgaatg agccagtcta tggcaccaag 840
cgtaaatctc aaattgagac atatttggag cacaatgaag gagctggact tcagcatctc 900
gcgctgaaga gtgaggacat cttccgcacc ctacgggaga tgaggaaaag aagtggtgtt 960
ggtgggtttg aattcatgcc atcacctcct gtcacctact acaggaacct gaagaacaga 1020
gttggtgatg tactttcgga cgagcagata aaggaatgtg aagagttggg gattttggtt 1080
gatagagatg atcaaggaac actcctgcag attgccacaa aaccaattgg cgaccgcccc 1140
actatcttca tagagatcat ccagagggtg gggtgcatgc tcaaagacga ggaagggaag 1200
gagtaccaga agggcggctg tggtggtttt gggaaaggaa acttttctga gttattcaaa 1260
tcaattgaag agtatgagaa gacgcttgaa acgcgtcgca ctgcttga 1308
<210> 41
<211> 435
<212> PRT
<213> 人工序列-MsHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 41
Met Ala Ile Glu Thr Glu Thr Gln Thr Gln Thr Gly Phe Lys Leu Val
1 5 10 15
Gly Phe Lys Asn Phe Val Arg Ala Asn Pro Lys Ser Asp Arg Phe Lys
20 25 30
Val Lys Arg Phe His His Val Glu Phe Trp Cys Thr Asp Ala Thr Asn
35 40 45
Thr Ala Arg Arg Phe Ser His Gly Leu Gly Met Pro Ile Val Ala Lys
50 55 60
Ser Asp Leu Ser Thr Gly Asn Gln Thr His Ala Ser Tyr Leu Leu Arg
65 70 75 80
Ser Gly Asp Leu Asn Phe Leu Phe Ser Ala Ala Tyr Ser Pro Ser Ile
85 90 95
Ser Leu Ser Ser Pro Ser Ser Thr Ala Ala Ile Pro Thr Phe Ser Ala
100 105 110
Ser Asn Cys Phe Ser Phe Ser Ala Ser His Gly Leu Ala Val Arg Ala
115 120 125
Val Ala Val Glu Val Glu Asp Ala Glu Leu Ala Phe Thr Thr Ser Val
130 135 140
Asn Asn Gly Ala Ile Pro Ser Ser Pro Pro Val Ile Leu Glu Asn His
145 150 155 160
Val Lys Leu Ala Glu Val Arg Leu Tyr Gly Asp Val Val Leu Arg Tyr
165 170 175
Val Ser Tyr Asn Asp Pro Asn Pro Asn Gln Asn Pro Asn Leu Leu Phe
180 185 190
Leu Pro Gly Phe Glu Arg Val Ser Asp Glu Ser Ser Asn Ser Ser Leu
195 200 205
Asp Phe Gly Ile Arg Arg Leu Asp His Ala Val Gly Asn Val Pro Glu
210 215 220
Leu Ser Ser Ala Val Lys Tyr Val Lys Asp Phe Thr Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Ser Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Glu Glu Thr Val Leu Leu Pro Met
260 265 270
Asn Glu Pro Val Tyr Gly Thr Lys Arg Lys Ser Gln Ile Glu Thr Tyr
275 280 285
Leu Glu His Asn Glu Gly Ala Gly Leu Gln His Leu Ala Leu Lys Ser
290 295 300
Glu Asp Ile Phe Arg Thr Leu Arg Glu Met Arg Lys Arg Ser Gly Val
305 310 315 320
Gly Gly Phe Glu Phe Met Pro Ser Pro Pro Val Thr Tyr Tyr Arg Asn
325 330 335
Leu Lys Asn Arg Val Gly Asp Val Leu Ser Asp Glu Gln Ile Lys Glu
340 345 350
Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp Gln Gly Thr Leu
355 360 365
Leu Gln Ile Phe Thr Lys Pro Ile Gly Asp Arg Pro Thr Ile Trp Ile
370 375 380
Glu Ile Ile Gln Arg Val Gly Cys Met Leu Lys Asp Glu Glu Gly Lys
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr Leu Glu Thr Arg
420 425 430
Arg Thr Ala
435
<210> 42
<211> 1308
<212> DNA
<213> 人工序列-MsHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 42
atggccatcg aaacagaaac tcaaacccaa accgggttca aactagttgg gttcaagaac 60
ttcgtccgag ccaatccaaa atcagaccgg ttcaaagtaa aacgcttcca ccatgttgag 120
ttctggtgca ccgacgcaac aaacaccgca cgccgtttct ctcacggact cggaatgccg 180
attgtcgcta aatcagacct ctccaccgga aatcaaactc acgcctccta tctcctccgc 240
tccggtgacc tcaacttcct cttctccgcc gcttattccc cttccatttc tctctcctca 300
ccttcctcaa ctgccgcaat ccccactttc tccgcatcca attgtttctc cttctccgct 360
tcccacggcc tcgccgtccg tgctgttgct gttgaagtcg aagacgccga actggctttc 420
accaccagcg taaacaacgg tgccattcca tcgtctcctc cagttatcct cgaaaaccac 480
gtcaaactgg ccgaagttcg tctctacggc gacgttgttc tacgttacgt cagttacaac 540
gacccgaacc caaaccagaa tccgaacctg ctcttcctcc ctggttttga acgtgtatca 600
gatgaatcat ctaattcatc gttagatttc ggaatccggc gactagacca tgccgttgga 660
aatgtaccgg agctttcatc cgctgtgaaa tacgtgaaag atttcaccgg atttcatgaa 720
tttgctgagt ttacagcgga agacgttgga acgagtgaga gtggattaaa ctcagtggtg 780
ttagctaaca atgaagaaac agtgttgctt ccgatgaatg aaccggttta tggaacaaag 840
aggaagagtc aaatagaaac gtatttggaa cataatgaag gtgctgggtt acaacatttg 900
gcattgaagt ctgaggatat atttaggaca ttgagggaaa tgagaaagag aagtggtgtt 960
ggtgggtttg agtttatgcc ttctcctccg gtcacttatt atcgtaattt gaagaatcgc 1020
gttggcgatg ttttgagtga tgaacagatt aaggagtgtg aagagcttgg gattttggtt 1080
gatagagatg atcagggtac tctgcttcag atttttacca agcctattgg agataggcca 1140
accatatgga tagagataat tcagagagtt ggatgcatgc tcaaggacga agaagggaag 1200
gagtatcaaa agggaggatg tgggggcttt ggaaaaggaa acttctcgga gcttttcaaa 1260
tccattgaag agtatgagaa gactttggaa actagaagaa ctgcatga 1308
<210> 43
<211> 1308
<212> DNA
<213> 人工序列-MsHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 43
atggctattg aaaccgaaac acaaactcag actggattta aactggtagg gttcaagaat 60
ttcgttaggg ctaatcccaa gtctgatcgt tttaaggtca agaggttcca ccacgtggag 120
ttctggtgca ctgatgcaac caacacggcg cggcgttttt cacatggtct tggaatgcct 180
attgttgcaa aatccgacct ctccacgggg aaccaaacac acgcctctta cctgctacgg 240
agtggtgact tgaacttcct cttcagcgcc gcctactcac cctccatatc gttgtcttca 300
ccatcctcga ccgcagcaat tcctacattt tctgcgagca actgcttctc tttctctgcc 360
agtcatggcc tcgccgtcag agcagtggcc gttgaagtag aggatgctga acttgctttt 420
acaacttcag tgaataatgg agcaataccg agcagtccgc cggtcatact tgagaatcat 480
gtgaagctag cggaggtgag gctttatggg gacgtcgttt taagatatgt cagctacaat 540
gatccaaacc ccaatcaaaa tccaaatttg ttgttccttc ctggcttcga gcgtgttagc 600
gatgaatctt caaactcttc actggatttt ggaatcagac ggttggatca tgctgttgga 660
aatgttcctg aactctcgag cgctgtgaaa tatgtgaagg acttcaccgg ttttcatgaa 720
tttgctgagt ttactgcaga agatgtgggt acatcagaga gtggcttgaa ttccgtggtg 780
ctcgccaaca acgaggagac tgtactctta ccaatgaatg agccagtcta tggcaccaag 840
cgtaaatctc aaattgagac atatttggag cacaatgaag gagctggact tcagcatctc 900
gcgctgaaga gtgaggacat cttccgcacc ctacgggaga tgaggaaaag aagtggtgtt 960
ggtgggtttg aattcatgcc atcacctcct gtcacctact acaggaacct gaagaacaga 1020
gttggtgatg tactttcgga cgagcagata aaggaatgtg aagagttggg gattttggtt 1080
gatagagatg atcaaggaac actcctgcag atttttacaa aaccaattgg cgaccgcccc 1140
actatctgga tagagatcat ccagagggtg gggtgcatgc tcaaagacga ggaagggaag 1200
gagtaccaga agggcggctg tggtggtttt gggaaaggaa acttttctga gttattcaaa 1260
tcaattgaag agtatgagaa gacgcttgaa acgcgtcgca ctgcttga 1308
<210> 44
<211> 435
<212> PRT
<213> 人工序列-MsHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 44
Met Ala Ile Glu Thr Glu Thr Gln Thr Gln Thr Gly Phe Lys Leu Val
1 5 10 15
Gly Phe Lys Asn Phe Val Arg Ala Asn Pro Lys Ser Asp Arg Phe Lys
20 25 30
Val Lys Arg Phe His His Val Glu Phe Trp Cys Thr Asp Ala Thr Asn
35 40 45
Thr Ala Arg Arg Phe Ser His Gly Leu Gly Met Pro Ile Val Ala Lys
50 55 60
Ser Asp Leu Ser Thr Gly Asn Gln Thr His Ala Ser Tyr Leu Leu Arg
65 70 75 80
Ser Gly Asp Leu Asn Phe Leu Phe Ser Ala Ala Tyr Ser Pro Ser Ile
85 90 95
Ser Leu Ser Ser Pro Ser Ser Thr Ala Ala Ile Pro Thr Phe Ser Ala
100 105 110
Ser Asn Cys Phe Ser Phe Ser Ala Ser His Gly Leu Ala Val Arg Ala
115 120 125
Val Ala Val Glu Val Glu Asp Ala Glu Leu Ala Phe Thr Thr Ser Val
130 135 140
Asn Asn Gly Ala Ile Pro Ser Ser Pro Pro Val Ile Leu Glu Asn His
145 150 155 160
Val Lys Leu Ala Glu Val Arg Leu Tyr Gly Asp Val Val Leu Arg Tyr
165 170 175
Val Ser Tyr Asn Asp Pro Asn Pro Asn Gln Asn Pro Asn Leu Leu Phe
180 185 190
Leu Pro Gly Phe Glu Arg Val Ser Asp Glu Ser Ser Asn Ser Ser Leu
195 200 205
Asp Phe Gly Ile Arg Arg Leu Asp His Ala Val Gly Asn Val Pro Glu
210 215 220
Leu Ser Ser Ala Val Lys Tyr Val Lys Asp Phe Thr Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Ser Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Glu Glu Thr Val Leu Leu Pro Met
260 265 270
Asn Glu Pro Val Tyr Gly Thr Lys Arg Lys Ser Gln Ile Glu Thr Tyr
275 280 285
Leu Glu His Asn Glu Gly Ala Gly Leu Gln His Leu Ala Leu Lys Ser
290 295 300
Glu Asp Ile Phe Arg Thr Leu Arg Glu Met Arg Lys Arg Ser Gly Val
305 310 315 320
Gly Gly Phe Glu Phe Met Pro Ser Pro Pro Val Thr Tyr Tyr Arg Asn
325 330 335
Leu Lys Asn Arg Val Gly Asp Val Leu Ser Asp Glu Gln Ile Lys Glu
340 345 350
Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp Gln Gly Thr Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Ile Gly Asp Arg Pro Thr Ile Trp Ile
370 375 380
Glu Ile Ile Gln Arg Val Gly Cys Met Leu Lys Asp Glu Glu Gly Lys
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr Leu Glu Thr Arg
420 425 430
Arg Thr Ala
435
<210> 45
<211> 1308
<212> DNA
<213> 人工序列-MsHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 45
atggccatcg aaacagaaac tcaaacccaa accgggttca aactagttgg gttcaagaac 60
ttcgtccgag ccaatccaaa atcagaccgg ttcaaagtaa aacgcttcca ccatgttgag 120
ttctggtgca ccgacgcaac aaacaccgca cgccgtttct ctcacggact cggaatgccg 180
attgtcgcta aatcagacct ctccaccgga aatcaaactc acgcctccta tctcctccgc 240
tccggtgacc tcaacttcct cttctccgcc gcttattccc cttccatttc tctctcctca 300
ccttcctcaa ctgccgcaat ccccactttc tccgcatcca attgtttctc cttctccgct 360
tcccacggcc tcgccgtccg tgctgttgct gttgaagtcg aagacgccga actggctttc 420
accaccagcg taaacaacgg tgccattcca tcgtctcctc cagttatcct cgaaaaccac 480
gtcaaactgg ccgaagttcg tctctacggc gacgttgttc tacgttacgt cagttacaac 540
gacccgaacc caaaccagaa tccgaacctg ctcttcctcc ctggttttga acgtgtatca 600
gatgaatcat ctaattcatc gttagatttc ggaatccggc gactagacca tgccgttgga 660
aatgtaccgg agctttcatc cgctgtgaaa tacgtgaaag atttcaccgg atttcatgaa 720
tttgctgagt ttacagcgga agacgttgga acgagtgaga gtggattaaa ctcagtggtg 780
ttagctaaca atgaagaaac agtgttgctt ccgatgaatg aaccggttta tggaacaaag 840
aggaagagtc aaatagaaac gtatttggaa cataatgaag gtgctgggtt acaacatttg 900
gcattgaagt ctgaggatat atttaggaca ttgagggaaa tgagaaagag aagtggtgtt 960
ggtgggtttg agtttatgcc ttctcctccg gtcacttatt atcgtaattt gaagaatcgc 1020
gttggcgatg ttttgagtga tgaacagatt aaggagtgtg aagagcttgg gattttggtt 1080
gatagagatg atcagggtac tctgcttcag attgctacca agcctattgg agataggcca 1140
accatatgga tagagataat tcagagagtt ggatgcatgc tcaaggacga agaagggaag 1200
gagtatcaaa agggaggatg tgggggcttt ggaaaaggaa acttctcgga gcttttcaaa 1260
tccattgaag agtatgagaa gactttggaa actagaagaa ctgcatga 1308
<210> 46
<211> 1308
<212> DNA
<213> 人工序列-MsHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 46
atggctattg aaaccgaaac acaaactcag actggattta aactggtagg gttcaagaat 60
ttcgttaggg ctaatcccaa gtctgatcgt tttaaggtca agaggttcca ccacgtggag 120
ttctggtgca ctgatgcaac caacacggcg cggcgttttt cacatggtct tggaatgcct 180
attgttgcaa aatccgacct ctccacgggg aaccaaacac acgcctctta cctgctacgg 240
agtggtgact tgaacttcct cttcagcgcc gcctactcac cctccatatc gttgtcttca 300
ccatcctcga ccgcagcaat tcctacattt tctgcgagca actgcttctc tttctctgcc 360
agtcatggcc tcgccgtcag agcagtggcc gttgaagtag aggatgctga acttgctttt 420
acaacttcag tgaataatgg agcaataccg agcagtccgc cggtcatact tgagaatcat 480
gtgaagctag cggaggtgag gctttatggg gacgtcgttt taagatatgt cagctacaat 540
gatccaaacc ccaatcaaaa tccaaatttg ttgttccttc ctggcttcga gcgtgttagc 600
gatgaatctt caaactcttc actggatttt ggaatcagac ggttggatca tgctgttgga 660
aatgttcctg aactctcgag cgctgtgaaa tatgtgaagg acttcaccgg ttttcatgaa 720
tttgctgagt ttactgcaga agatgtgggt acatcagaga gtggcttgaa ttccgtggtg 780
ctcgccaaca acgaggagac tgtactctta ccaatgaatg agccagtcta tggcaccaag 840
cgtaaatctc aaattgagac atatttggag cacaatgaag gagctggact tcagcatctc 900
gcgctgaaga gtgaggacat cttccgcacc ctacgggaga tgaggaaaag aagtggtgtt 960
ggtgggtttg aattcatgcc atcacctcct gtcacctact acaggaacct gaagaacaga 1020
gttggtgatg tactttcgga cgagcagata aaggaatgtg aagagttggg gattttggtt 1080
gatagagatg atcaaggaac actcctgcag attgccacaa aaccaattgg cgaccgcccc 1140
actatctgga tagagatcat ccagagggtg gggtgcatgc tcaaagacga ggaagggaag 1200
gagtaccaga agggcggctg tggtggtttt gggaaaggaa acttttctga gttattcaaa 1260
tcaattgaag agtatgagaa gacgcttgaa acgcgtcgca ctgcttga 1308
<210> 47
<211> 436
<212> PRT
<213> 棉花野生型HPPD(GsHPPD的氨基酸序列Gossypium hirsutum)
<400> 47
Met Val Gln Thr Asn Gln Ser Gly Ser Gly Asn Asp Phe Lys Leu Val
1 5 10 15
Gly Phe Ser Asn Phe Val Arg Ser Asn Pro Lys Ser Asp Arg Phe Thr
20 25 30
Val Lys Arg Phe His His Ile Glu Phe Trp Cys Thr Asp Ala Thr Asn
35 40 45
Val Ala Arg Arg Phe Ser Trp Gly Leu Gly Met Gln Phe Val Ala Lys
50 55 60
Ser Asp Leu Ser Thr Gly Asn Leu Thr His Ala Ser Tyr Leu Leu Arg
65 70 75 80
Ser Arg Asp Leu Asn Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Ile
85 90 95
Ala Val Ala Gln Asn Leu Ser Pro Gln Ser Thr Ala Ser Ile Pro Ser
100 105 110
Phe Asp His Ser Leu Cys Arg Ser Phe Ala Ala Thr His Gly Leu Gly
115 120 125
Val Arg Ala Ile Ala Ile Glu Val Asp Asp Ala Glu Thr Ala Phe Thr
130 135 140
Thr Ser Val Thr His Gly Ala Leu Pro Phe Cys Pro Pro Thr Pro Leu
145 150 155 160
Gly Asp Val Ala Thr Ile Ala Glu Val Lys Leu Tyr Gly Asp Val Val
165 170 175
Leu Arg Tyr Val Ser Tyr Thr Thr Thr Ile Asn Ser Asp His Asp Phe
180 185 190
Leu Pro Gly Phe Glu Lys Ile Glu Asp Thr Leu Ser Tyr Pro Leu Asp
195 200 205
Tyr Gly Leu Arg Arg Leu Asp His Ala Val Gly Asn Val Pro Glu Leu
210 215 220
Gly Pro Ala Val Ser Tyr Val Lys Ser Phe Thr Gly Phe His Glu Phe
225 230 235 240
Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Ser Glu Ser Gly Leu Asn
245 250 255
Ser Val Val Leu Ala Asn Asn Glu Glu Met Val Leu Leu Pro Met Asn
260 265 270
Glu Pro Val Phe Gly Thr Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu
275 280 285
Glu His Asn Glu Gly Ala Gly Val Gln His Leu Ala Leu Val Ser Glu
290 295 300
Asp Ile Phe Lys Thr Leu Arg Glu Met Arg Lys Arg Ser Phe Val Gly
305 310 315 320
Gly Phe Glu Phe Met Pro Ser Pro Pro Pro Thr Tyr Tyr Lys Lys Leu
325 330 335
Lys Gln Arg Ala Gly Asp Ile Leu Ser Asp Glu Gln Ile Lys Glu Cys
340 345 350
Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp Gln Gly Thr Leu Leu
355 360 365
Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Ile Phe Ile Glu
370 375 380
Ile Ile Gln Arg Ile Gly Cys Met Val Lys Asp Glu Glu Gly Lys Gln
385 390 395 400
Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu
405 410 415
Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Ser Leu Glu Ala Lys Gln
420 425 430
Ser Gln Asn Pro
435
<210> 48
<211> 1311
<212> DNA
<213> GsHPPD-01核苷酸序列(Gossypium hirsutum)
<400> 48
atggtccaga caaaccagtc tggttccggc aatgatttca agctcgtcgg attctcgaat 60
ttcgtccggt caaatccaaa atccgatcgg ttcaccgtca aacgtttcca ccacatcgag 120
ttttggtgta ccgacgccac taacgtcgct cgccgttttt catggggtct cggtatgcaa 180
tttgtagcta aatcggattt atccaccgga aatttgaccc atgcttctta tctcctccgt 240
tcccgtgacc tgaacttcct tttcactgcc ccctattccc cttctattgc cgttgctcaa 300
aacctctccc ctcaatccac cgcttcgatc ccttcttttg atcattcact ctgccgctcc 360
ttcgctgcca cccacggctt aggcgttcgc gccatcgcca tcgaagttga cgatgccgaa 420
accgctttca ccaccagtgt cacacatggc gcgttaccct tttgccctcc taccccactc 480
ggcgacgtcg ccactatcgc cgaagtcaaa ctctacggcg acgtcgtttt gcgttacgtc 540
agttacacca ccaccataaa ctccgaccat gatttcttgc cgggattcga gaaaatagaa 600
gacacccttt cttacccttt agattacgga ctccgacgac tcgaccacgc cgtcggcaac 660
gtcccagaac tcggtcccgc tgtttcgtac gttaaatcct tcaccggctt ccacgaattc 720
gctgaattca cagctgaaga cgtcggaact agcgaaagtg gactaaactc cgtcgtttta 780
gctaacaacg aagaaatggt attacttccg atgaacgaac cggtgttcgg aacaaaaagg 840
aaaagccaaa tccaaacgta tttagaacac aacgaaggtg ccggagttca acatttggca 900
ttggtgagtg aagatatatt caagacgtta agagaaatga ggaagagaag cttcgtcggc 960
ggttttgagt tcatgccgtc gccgccgccg acttattata aaaaattgaa gcaaagggca 1020
ggggatattt tgagtgatga acagattaaa gagtgtgaag aactggggat tctggttgat 1080
agagatgatc aagggacttt gctgcaaatt ttcactaagc cagttggtga taggccaacc 1140
atcttcatag agataataca aagaattggg tgcatggtga aggatgaaga aggaaagcaa 1200
taccaaaaag gtggatgtgg tggttttggg aaaggcaact tttctgagct cttcaaatcc 1260
attgaagaat atgagaaatc tcttgaagcc aaacaatctc agaatccatg a 1311
<210> 49
<211> 1311
<212> DNA
<213> 人工序列-GsHPPD-02核苷酸序列(Artificial Sequence)
<400> 49
atggttcaaa caaatcaatc tggttctggg aatgatttca agctggttgg tttctcaaat 60
tttgtgcgta gcaatcccaa atcagaccgc ttcacggtca agagattcca ccacattgaa 120
ttttggtgca ctgatgccac taatgtggcg aggaggtttt catggggttt agggatgcag 180
ttcgtcgcca agagtgacct ctccacagga aatttgacgc atgcaagcta cctgctgcgg 240
agccgcgact tgaatttcct gtttacagca ccatattcac ccagtattgc agttgctcaa 300
aacctttcgc cacaaagcac tgcttccata ccttcatttg atcactcgct ttgtcggtcc 360
tttgctgcaa cacatggact tggcgtcaga gcaattgcca tcgaggtgga cgacgccgaa 420
actgccttca ccacgagcgt gacacatggc gcgctccctt tttgtccacc aacaccacta 480
ggagatgtgg ctacaattgc tgaagttaaa ttatatggtg atgttgtgct cagatatgtt 540
tcttacacca caaccatcaa ctccgaccat gattttctgc ctggttttga gaagattgaa 600
gatactctat cgtatccttt ggattatggc ctgcgtcggc tcgatcatgc tgttggcaac 660
gtacctgaat taggtcctgc tgtatcttat gtgaaatctt ttactgggtt ccatgagttt 720
gcagagttca ccgctgagga tgtaggaaca agtgaatctg ggctgaactc agttgttttg 780
gccaacaatg aggaaatggt cctcttacca atgaacgagc cggtgttcgg cactaagcgc 840
aaatctcaaa tacagaccta cttggagcac aatgaaggag ctggtgtgca gcacctcgcc 900
ctcgtctcgg aggacatctt caaaacttta agggagatga ggaaacgatc ctttgtggga 960
ggatttgaat tcatgccttc tccgccgccg acatactaca agaagctcaa gcagcgcgcg 1020
ggggacattt tgtctgatga gcagatcaag gagtgtgaag agcttgggat tcttgttgat 1080
agagatgatc aaggaacgct tctgcagata tttaccaagc ccgtcggcga ccggcctact 1140
atattcattg agatcatcca gagaattggt tgcatggtga aggatgaaga agggaaacag 1200
taccagaagg gcggctgcgg cgggtttgga aaaggaaact tcagtgagct gtttaaaagt 1260
atagaggagt atgaaaagag cttggaagca aaacaatcac aaaatccatg a 1311
<210> 50
<211> 436
<212> PRT
<213> 人工序列-GsHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 50
Met Val Gln Thr Asn Gln Ser Gly Ser Gly Asn Asp Phe Lys Leu Val
1 5 10 15
Gly Phe Ser Asn Phe Val Arg Ser Asn Pro Lys Ser Asp Arg Phe Thr
20 25 30
Val Lys Arg Phe His His Ile Glu Phe Trp Cys Thr Asp Ala Thr Asn
35 40 45
Val Ala Arg Arg Phe Ser Trp Gly Leu Gly Met Gln Phe Val Ala Lys
50 55 60
Ser Asp Leu Ser Thr Gly Asn Leu Thr His Ala Ser Tyr Leu Leu Arg
65 70 75 80
Ser Arg Asp Leu Asn Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Ile
85 90 95
Ala Val Ala Gln Asn Leu Ser Pro Gln Ser Thr Ala Ser Ile Pro Ser
100 105 110
Phe Asp His Ser Leu Cys Arg Ser Phe Ala Ala Thr His Gly Leu Gly
115 120 125
Val Arg Ala Ile Ala Ile Glu Val Asp Asp Ala Glu Thr Ala Phe Thr
130 135 140
Thr Ser Val Thr His Gly Ala Leu Pro Phe Cys Pro Pro Thr Pro Leu
145 150 155 160
Gly Asp Val Ala Thr Ile Ala Glu Val Lys Leu Tyr Gly Asp Val Val
165 170 175
Leu Arg Tyr Val Ser Tyr Thr Thr Thr Ile Asn Ser Asp His Asp Phe
180 185 190
Leu Pro Gly Phe Glu Lys Ile Glu Asp Thr Leu Ser Tyr Pro Leu Asp
195 200 205
Tyr Gly Leu Arg Arg Leu Asp His Ala Val Gly Asn Val Pro Glu Leu
210 215 220
Gly Pro Ala Val Ser Tyr Val Lys Ser Phe Thr Gly Phe His Glu Phe
225 230 235 240
Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Ser Glu Ser Gly Leu Asn
245 250 255
Ser Val Val Leu Ala Asn Asn Glu Glu Met Val Leu Leu Pro Met Asn
260 265 270
Glu Pro Val Phe Gly Thr Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu
275 280 285
Glu His Asn Glu Gly Ala Gly Val Gln His Leu Ala Leu Val Ser Glu
290 295 300
Asp Ile Phe Lys Thr Leu Arg Glu Met Arg Lys Arg Ser Phe Val Gly
305 310 315 320
Gly Phe Glu Phe Met Pro Ser Pro Pro Pro Thr Tyr Tyr Lys Lys Leu
325 330 335
Lys Gln Arg Ala Gly Asp Ile Leu Ser Asp Glu Gln Ile Lys Glu Cys
340 345 350
Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp Gln Gly Thr Leu Leu
355 360 365
Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Ile Phe Ile Glu
370 375 380
Ile Ile Gln Arg Ile Gly Cys Met Val Lys Asp Glu Glu Gly Lys Gln
385 390 395 400
Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu
405 410 415
Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Ser Leu Glu Ala Lys Gln
420 425 430
Ser Gln Asn Pro
435
<210> 51
<211> 1311
<212> DNA
<213> 人工序列-GsHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 51
atggtccaga caaaccagtc tggttccggc aatgatttca agctcgtcgg attctcgaat 60
ttcgtccggt caaatccaaa atccgatcgg ttcaccgtca aacgtttcca ccacatcgag 120
ttttggtgta ccgacgccac taacgtcgct cgccgttttt catggggtct cggtatgcaa 180
tttgtagcta aatcggattt atccaccgga aatttgaccc atgcttctta tctcctccgt 240
tcccgtgacc tgaacttcct tttcactgcc ccctattccc cttctattgc cgttgctcaa 300
aacctctccc ctcaatccac cgcttcgatc ccttcttttg atcattcact ctgccgctcc 360
ttcgctgcca cccacggctt aggcgttcgc gccatcgcca tcgaagttga cgatgccgaa 420
accgctttca ccaccagtgt cacacatggc gcgttaccct tttgccctcc taccccactc 480
ggcgacgtcg ccactatcgc cgaagtcaaa ctctacggcg acgtcgtttt gcgttacgtc 540
agttacacca ccaccataaa ctccgaccat gatttcttgc cgggattcga gaaaatagaa 600
gacacccttt cttacccttt agattacgga ctccgacgac tcgaccacgc cgtcggcaac 660
gtcccagaac tcggtcccgc tgtttcgtac gttaaatcct tcaccggctt ccacgaattc 720
gctgaattca cagctgaaga cgtcggaact agcgaaagtg gactaaactc cgtcgtttta 780
gctaacaacg aagaaatggt attacttccg atgaacgaac cggtgttcgg aacaaaaagg 840
aaaagccaaa tccaaacgta tttagaacac aacgaaggtg ccggagttca acatttggca 900
ttggtgagtg aagatatatt caagacgtta agagaaatga ggaagagaag cttcgtcggc 960
ggttttgagt tcatgccgtc gccgccgccg acttattata aaaaattgaa gcaaagggca 1020
ggggatattt tgagtgatga acagattaaa gagtgtgaag aactggggat tctggttgat 1080
agagatgatc aagggacttt gctgcaaatt gctactaagc cagttggtga taggccaacc 1140
atcttcatag agataataca aagaattggg tgcatggtga aggatgaaga aggaaagcaa 1200
taccaaaaag gtggatgtgg tggttttggg aaaggcaact tttctgagct cttcaaatcc 1260
attgaagaat atgagaaatc tcttgaagcc aaacaatctc agaatccatg a 1311
<210> 52
<211> 1311
<212> DNA
<213> 人工序列-GsHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 52
atggttcaaa caaatcaatc tggttctggg aatgatttca agctggttgg tttctcaaat 60
tttgtgcgta gcaatcccaa atcagaccgc ttcacggtca agagattcca ccacattgaa 120
ttttggtgca ctgatgccac taatgtggcg aggaggtttt catggggttt agggatgcag 180
ttcgtcgcca agagtgacct ctccacagga aatttgacgc atgcaagcta cctgctgcgg 240
agccgcgact tgaatttcct gtttacagca ccatattcac ccagtattgc agttgctcaa 300
aacctttcgc cacaaagcac tgcttccata ccttcatttg atcactcgct ttgtcggtcc 360
tttgctgcaa cacatggact tggcgtcaga gcaattgcca tcgaggtgga cgacgccgaa 420
actgccttca ccacgagcgt gacacatggc gcgctccctt tttgtccacc aacaccacta 480
ggagatgtgg ctacaattgc tgaagttaaa ttatatggtg atgttgtgct cagatatgtt 540
tcttacacca caaccatcaa ctccgaccat gattttctgc ctggttttga gaagattgaa 600
gatactctat cgtatccttt ggattatggc ctgcgtcggc tcgatcatgc tgttggcaac 660
gtacctgaat taggtcctgc tgtatcttat gtgaaatctt ttactgggtt ccatgagttt 720
gcagagttca ccgctgagga tgtaggaaca agtgaatctg ggctgaactc agttgttttg 780
gccaacaatg aggaaatggt cctcttacca atgaacgagc cggtgttcgg cactaagcgc 840
aaatctcaaa tacagaccta cttggagcac aatgaaggag ctggtgtgca gcacctcgcc 900
ctcgtctcgg aggacatctt caaaacttta agggagatga ggaaacgatc ctttgtggga 960
ggatttgaat tcatgccttc tccgccgccg acatactaca agaagctcaa gcagcgcgcg 1020
ggggacattt tgtctgatga gcagatcaag gagtgtgaag agcttgggat tcttgttgat 1080
agagatgatc aaggaacgct tctgcagata gccaccaagc ccgtcggcga ccggcctact 1140
atattcattg agatcatcca gagaattggt tgcatggtga aggatgaaga agggaaacag 1200
taccagaagg gcggctgcgg cgggtttgga aaaggaaact tcagtgagct gtttaaaagt 1260
atagaggagt atgaaaagag cttggaagca aaacaatcac aaaatccatg a 1311
<210> 53
<211> 436
<212> PRT
<213> 人工序列-GsHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 53
Met Val Gln Thr Asn Gln Ser Gly Ser Gly Asn Asp Phe Lys Leu Val
1 5 10 15
Gly Phe Ser Asn Phe Val Arg Ser Asn Pro Lys Ser Asp Arg Phe Thr
20 25 30
Val Lys Arg Phe His His Ile Glu Phe Trp Cys Thr Asp Ala Thr Asn
35 40 45
Val Ala Arg Arg Phe Ser Trp Gly Leu Gly Met Gln Phe Val Ala Lys
50 55 60
Ser Asp Leu Ser Thr Gly Asn Leu Thr His Ala Ser Tyr Leu Leu Arg
65 70 75 80
Ser Arg Asp Leu Asn Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Ile
85 90 95
Ala Val Ala Gln Asn Leu Ser Pro Gln Ser Thr Ala Ser Ile Pro Ser
100 105 110
Phe Asp His Ser Leu Cys Arg Ser Phe Ala Ala Thr His Gly Leu Gly
115 120 125
Val Arg Ala Ile Ala Ile Glu Val Asp Asp Ala Glu Thr Ala Phe Thr
130 135 140
Thr Ser Val Thr His Gly Ala Leu Pro Phe Cys Pro Pro Thr Pro Leu
145 150 155 160
Gly Asp Val Ala Thr Ile Ala Glu Val Lys Leu Tyr Gly Asp Val Val
165 170 175
Leu Arg Tyr Val Ser Tyr Thr Thr Thr Ile Asn Ser Asp His Asp Phe
180 185 190
Leu Pro Gly Phe Glu Lys Ile Glu Asp Thr Leu Ser Tyr Pro Leu Asp
195 200 205
Tyr Gly Leu Arg Arg Leu Asp His Ala Val Gly Asn Val Pro Glu Leu
210 215 220
Gly Pro Ala Val Ser Tyr Val Lys Ser Phe Thr Gly Phe His Glu Phe
225 230 235 240
Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Ser Glu Ser Gly Leu Asn
245 250 255
Ser Val Val Leu Ala Asn Asn Glu Glu Met Val Leu Leu Pro Met Asn
260 265 270
Glu Pro Val Phe Gly Thr Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu
275 280 285
Glu His Asn Glu Gly Ala Gly Val Gln His Leu Ala Leu Val Ser Glu
290 295 300
Asp Ile Phe Lys Thr Leu Arg Glu Met Arg Lys Arg Ser Phe Val Gly
305 310 315 320
Gly Phe Glu Phe Met Pro Ser Pro Pro Pro Thr Tyr Tyr Lys Lys Leu
325 330 335
Lys Gln Arg Ala Gly Asp Ile Leu Ser Asp Glu Gln Ile Lys Glu Cys
340 345 350
Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp Gln Gly Thr Leu Leu
355 360 365
Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Ile Trp Ile Glu
370 375 380
Ile Ile Gln Arg Ile Gly Cys Met Val Lys Asp Glu Glu Gly Lys Gln
385 390 395 400
Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu
405 410 415
Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Ser Leu Glu Ala Lys Gln
420 425 430
Ser Gln Asn Pro
435
<210> 54
<211> 1311
<212> DNA
<213> 人工序列-GsHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 54
atggtccaga caaaccagtc tggttccggc aatgatttca agctcgtcgg attctcgaat 60
ttcgtccggt caaatccaaa atccgatcgg ttcaccgtca aacgtttcca ccacatcgag 120
ttttggtgta ccgacgccac taacgtcgct cgccgttttt catggggtct cggtatgcaa 180
tttgtagcta aatcggattt atccaccgga aatttgaccc atgcttctta tctcctccgt 240
tcccgtgacc tgaacttcct tttcactgcc ccctattccc cttctattgc cgttgctcaa 300
aacctctccc ctcaatccac cgcttcgatc ccttcttttg atcattcact ctgccgctcc 360
ttcgctgcca cccacggctt aggcgttcgc gccatcgcca tcgaagttga cgatgccgaa 420
accgctttca ccaccagtgt cacacatggc gcgttaccct tttgccctcc taccccactc 480
ggcgacgtcg ccactatcgc cgaagtcaaa ctctacggcg acgtcgtttt gcgttacgtc 540
agttacacca ccaccataaa ctccgaccat gatttcttgc cgggattcga gaaaatagaa 600
gacacccttt cttacccttt agattacgga ctccgacgac tcgaccacgc cgtcggcaac 660
gtcccagaac tcggtcccgc tgtttcgtac gttaaatcct tcaccggctt ccacgaattc 720
gctgaattca cagctgaaga cgtcggaact agcgaaagtg gactaaactc cgtcgtttta 780
gctaacaacg aagaaatggt attacttccg atgaacgaac cggtgttcgg aacaaaaagg 840
aaaagccaaa tccaaacgta tttagaacac aacgaaggtg ccggagttca acatttggca 900
ttggtgagtg aagatatatt caagacgtta agagaaatga ggaagagaag cttcgtcggc 960
ggttttgagt tcatgccgtc gccgccgccg acttattata aaaaattgaa gcaaagggca 1020
ggggatattt tgagtgatga acagattaaa gagtgtgaag aactggggat tctggttgat 1080
agagatgatc aagggacttt gctgcaaatt ttcactaagc cagttggtga taggccaacc 1140
atctggatag agataataca aagaattggg tgcatggtga aggatgaaga aggaaagcaa 1200
taccaaaaag gtggatgtgg tggttttggg aaaggcaact tttctgagct cttcaaatcc 1260
attgaagaat atgagaaatc tcttgaagcc aaacaatctc agaatccatg a 1311
<210> 55
<211> 1311
<212> DNA
<213> 人工序列-GsHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 55
atggttcaaa caaatcaatc tggttctggg aatgatttca agctggttgg tttctcaaat 60
tttgtgcgta gcaatcccaa atcagaccgc ttcacggtca agagattcca ccacattgaa 120
ttttggtgca ctgatgccac taatgtggcg aggaggtttt catggggttt agggatgcag 180
ttcgtcgcca agagtgacct ctccacagga aatttgacgc atgcaagcta cctgctgcgg 240
agccgcgact tgaatttcct gtttacagca ccatattcac ccagtattgc agttgctcaa 300
aacctttcgc cacaaagcac tgcttccata ccttcatttg atcactcgct ttgtcggtcc 360
tttgctgcaa cacatggact tggcgtcaga gcaattgcca tcgaggtgga cgacgccgaa 420
actgccttca ccacgagcgt gacacatggc gcgctccctt tttgtccacc aacaccacta 480
ggagatgtgg ctacaattgc tgaagttaaa ttatatggtg atgttgtgct cagatatgtt 540
tcttacacca caaccatcaa ctccgaccat gattttctgc ctggttttga gaagattgaa 600
gatactctat cgtatccttt ggattatggc ctgcgtcggc tcgatcatgc tgttggcaac 660
gtacctgaat taggtcctgc tgtatcttat gtgaaatctt ttactgggtt ccatgagttt 720
gcagagttca ccgctgagga tgtaggaaca agtgaatctg ggctgaactc agttgttttg 780
gccaacaatg aggaaatggt cctcttacca atgaacgagc cggtgttcgg cactaagcgc 840
aaatctcaaa tacagaccta cttggagcac aatgaaggag ctggtgtgca gcacctcgcc 900
ctcgtctcgg aggacatctt caaaacttta agggagatga ggaaacgatc ctttgtggga 960
ggatttgaat tcatgccttc tccgccgccg acatactaca agaagctcaa gcagcgcgcg 1020
ggggacattt tgtctgatga gcagatcaag gagtgtgaag agcttgggat tcttgttgat 1080
agagatgatc aaggaacgct tctgcagata tttaccaagc ccgtcggcga ccggcctact 1140
atatggattg agatcatcca gagaattggt tgcatggtga aggatgaaga agggaaacag 1200
taccagaagg gcggctgcgg cgggtttgga aaaggaaact tcagtgagct gtttaaaagt 1260
atagaggagt atgaaaagag cttggaagca aaacaatcac aaaatccatg a 1311
<210> 56
<211> 436
<212> PRT
<213> 人工序列-GsHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 56
Met Val Gln Thr Asn Gln Ser Gly Ser Gly Asn Asp Phe Lys Leu Val
1 5 10 15
Gly Phe Ser Asn Phe Val Arg Ser Asn Pro Lys Ser Asp Arg Phe Thr
20 25 30
Val Lys Arg Phe His His Ile Glu Phe Trp Cys Thr Asp Ala Thr Asn
35 40 45
Val Ala Arg Arg Phe Ser Trp Gly Leu Gly Met Gln Phe Val Ala Lys
50 55 60
Ser Asp Leu Ser Thr Gly Asn Leu Thr His Ala Ser Tyr Leu Leu Arg
65 70 75 80
Ser Arg Asp Leu Asn Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Ile
85 90 95
Ala Val Ala Gln Asn Leu Ser Pro Gln Ser Thr Ala Ser Ile Pro Ser
100 105 110
Phe Asp His Ser Leu Cys Arg Ser Phe Ala Ala Thr His Gly Leu Gly
115 120 125
Val Arg Ala Ile Ala Ile Glu Val Asp Asp Ala Glu Thr Ala Phe Thr
130 135 140
Thr Ser Val Thr His Gly Ala Leu Pro Phe Cys Pro Pro Thr Pro Leu
145 150 155 160
Gly Asp Val Ala Thr Ile Ala Glu Val Lys Leu Tyr Gly Asp Val Val
165 170 175
Leu Arg Tyr Val Ser Tyr Thr Thr Thr Ile Asn Ser Asp His Asp Phe
180 185 190
Leu Pro Gly Phe Glu Lys Ile Glu Asp Thr Leu Ser Tyr Pro Leu Asp
195 200 205
Tyr Gly Leu Arg Arg Leu Asp His Ala Val Gly Asn Val Pro Glu Leu
210 215 220
Gly Pro Ala Val Ser Tyr Val Lys Ser Phe Thr Gly Phe His Glu Phe
225 230 235 240
Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Ser Glu Ser Gly Leu Asn
245 250 255
Ser Val Val Leu Ala Asn Asn Glu Glu Met Val Leu Leu Pro Met Asn
260 265 270
Glu Pro Val Phe Gly Thr Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu
275 280 285
Glu His Asn Glu Gly Ala Gly Val Gln His Leu Ala Leu Val Ser Glu
290 295 300
Asp Ile Phe Lys Thr Leu Arg Glu Met Arg Lys Arg Ser Phe Val Gly
305 310 315 320
Gly Phe Glu Phe Met Pro Ser Pro Pro Pro Thr Tyr Tyr Lys Lys Leu
325 330 335
Lys Gln Arg Ala Gly Asp Ile Leu Ser Asp Glu Gln Ile Lys Glu Cys
340 345 350
Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp Gln Gly Thr Leu Leu
355 360 365
Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Ile Trp Ile Glu
370 375 380
Ile Ile Gln Arg Ile Gly Cys Met Val Lys Asp Glu Glu Gly Lys Gln
385 390 395 400
Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu
405 410 415
Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Ser Leu Glu Ala Lys Gln
420 425 430
Ser Gln Asn Pro
435
<210> 57
<211> 1311
<212> DNA
<213> 人工序列-GsHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 57
atggtccaga caaaccagtc tggttccggc aatgatttca agctcgtcgg attctcgaat 60
ttcgtccggt caaatccaaa atccgatcgg ttcaccgtca aacgtttcca ccacatcgag 120
ttttggtgta ccgacgccac taacgtcgct cgccgttttt catggggtct cggtatgcaa 180
tttgtagcta aatcggattt atccaccgga aatttgaccc atgcttctta tctcctccgt 240
tcccgtgacc tgaacttcct tttcactgcc ccctattccc cttctattgc cgttgctcaa 300
aacctctccc ctcaatccac cgcttcgatc ccttcttttg atcattcact ctgccgctcc 360
ttcgctgcca cccacggctt aggcgttcgc gccatcgcca tcgaagttga cgatgccgaa 420
accgctttca ccaccagtgt cacacatggc gcgttaccct tttgccctcc taccccactc 480
ggcgacgtcg ccactatcgc cgaagtcaaa ctctacggcg acgtcgtttt gcgttacgtc 540
agttacacca ccaccataaa ctccgaccat gatttcttgc cgggattcga gaaaatagaa 600
gacacccttt cttacccttt agattacgga ctccgacgac tcgaccacgc cgtcggcaac 660
gtcccagaac tcggtcccgc tgtttcgtac gttaaatcct tcaccggctt ccacgaattc 720
gctgaattca cagctgaaga cgtcggaact agcgaaagtg gactaaactc cgtcgtttta 780
gctaacaacg aagaaatggt attacttccg atgaacgaac cggtgttcgg aacaaaaagg 840
aaaagccaaa tccaaacgta tttagaacac aacgaaggtg ccggagttca acatttggca 900
ttggtgagtg aagatatatt caagacgtta agagaaatga ggaagagaag cttcgtcggc 960
ggttttgagt tcatgccgtc gccgccgccg acttattata aaaaattgaa gcaaagggca 1020
ggggatattt tgagtgatga acagattaaa gagtgtgaag aactggggat tctggttgat 1080
agagatgatc aagggacttt gctgcaaatt gctactaagc cagttggtga taggccaacc 1140
atctggatag agataataca aagaattggg tgcatggtga aggatgaaga aggaaagcaa 1200
taccaaaaag gtggatgtgg tggttttggg aaaggcaact tttctgagct cttcaaatcc 1260
attgaagaat atgagaaatc tcttgaagcc aaacaatctc agaatccatg a 1311
<210> 58
<211> 1311
<212> DNA
<213> 人工序列-GsHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 58
atggttcaaa caaatcaatc tggttctggg aatgatttca agctggttgg tttctcaaat 60
tttgtgcgta gcaatcccaa atcagaccgc ttcacggtca agagattcca ccacattgaa 120
ttttggtgca ctgatgccac taatgtggcg aggaggtttt catggggttt agggatgcag 180
ttcgtcgcca agagtgacct ctccacagga aatttgacgc atgcaagcta cctgctgcgg 240
agccgcgact tgaatttcct gtttacagca ccatattcac ccagtattgc agttgctcaa 300
aacctttcgc cacaaagcac tgcttccata ccttcatttg atcactcgct ttgtcggtcc 360
tttgctgcaa cacatggact tggcgtcaga gcaattgcca tcgaggtgga cgacgccgaa 420
actgccttca ccacgagcgt gacacatggc gcgctccctt tttgtccacc aacaccacta 480
ggagatgtgg ctacaattgc tgaagttaaa ttatatggtg atgttgtgct cagatatgtt 540
tcttacacca caaccatcaa ctccgaccat gattttctgc ctggttttga gaagattgaa 600
gatactctat cgtatccttt ggattatggc ctgcgtcggc tcgatcatgc tgttggcaac 660
gtacctgaat taggtcctgc tgtatcttat gtgaaatctt ttactgggtt ccatgagttt 720
gcagagttca ccgctgagga tgtaggaaca agtgaatctg ggctgaactc agttgttttg 780
gccaacaatg aggaaatggt cctcttacca atgaacgagc cggtgttcgg cactaagcgc 840
aaatctcaaa tacagaccta cttggagcac aatgaaggag ctggtgtgca gcacctcgcc 900
ctcgtctcgg aggacatctt caaaacttta agggagatga ggaaacgatc ctttgtggga 960
ggatttgaat tcatgccttc tccgccgccg acatactaca agaagctcaa gcagcgcgcg 1020
ggggacattt tgtctgatga gcagatcaag gagtgtgaag agcttgggat tcttgttgat 1080
agagatgatc aaggaacgct tctgcagata gccaccaagc ccgtcggcga ccggcctact 1140
atatggattg agatcatcca gagaattggt tgcatggtga aggatgaaga agggaaacag 1200
taccagaagg gcggctgcgg cgggtttgga aaaggaaact tcagtgagct gtttaaaagt 1260
atagaggagt atgaaaagag cttggaagca aaacaatcac aaaatccatg a 1311
<210> 59
<211> 440
<212> PRT
<213> 油菜野生型HPPD(BnHPPD的氨基酸序列Brassica napus)
<400> 59
Met Gly His Glu Asn Ala Ala Val Cys Glu Thr Gln Gln His Asp Asp
1 5 10 15
Ala Ala Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe Val Arg
20 25 30
Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His His Ile
35 40 45
Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ser Arg Arg Phe Ser Trp
50 55 60
Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr Gly Asn
65 70 75 80
Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg Phe Leu
85 90 95
Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ala Gln Pro
100 105 110
Ser Ala Thr Ala Ser Ile Pro Ser Phe Asp His Ala Ser Cys Arg Ser
115 120 125
Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile Glu Val
130 135 140
Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly Ala Val
145 150 155 160
Pro Ser Ser Pro Pro Asn Val Leu Asn Gly Ala Val Thr Ile Ala Glu
165 170 175
Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Tyr Asn
180 185 190
Gly Thr Val Ser Phe Leu Pro Gly Phe Glu Ser Val Asp Asp Thr Ser
195 200 205
Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu Asp His Ala Val Gly
210 215 220
Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr Leu Ala Gly Phe Thr
225 230 235 240
Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp Asp Val Gly Thr Ala
245 250 255
Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Asn Asn Asp Glu Met Val
260 265 270
Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr Lys Arg Lys Ser Gln
275 280 285
Ile Gln Thr Phe Leu Glu His Asn Glu Gly Ala Gly Leu Gln His Leu
290 295 300
Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu Arg Glu Met Arg Lys
305 310 315 320
Arg Ser Gly Val Gly Gly Phe Asp Phe Met Pro Ser Pro Pro Pro Thr
325 330 335
Tyr Tyr Lys Asn Leu Lys Lys Arg Val Gly Asp Val Leu Ser Glu Glu
340 345 350
Gln Ile Glu Glu Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp
355 360 365
Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro Leu Gly Asp Arg Pro
370 375 380
Thr Ile Phe Ile Glu Ile Ile Gln Arg Val Gly Cys Met Lys Arg Asp
385 390 395 400
Glu Glu Gly Lys Val Tyr Gln Ser Gly Gly Cys Gly Gly Phe Gly Lys
405 410 415
Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr
420 425 430
Leu Glu Ala Lys Gln Leu Val Gly
435 440
<210> 60
<211> 1323
<212> DNA
<213> BnHPPD-01核苷酸序列(Brassica napus)
<400> 60
atggggcacg aaaacgccgc cgtttgcgaa actcagcagc acgacgacgc tgcgtcgcct 60
ggattcaagc tcgtcggatt ctccaagttc gtgaggaaga atccaaagtc cgacaagttc 120
aaggtaaagc gcttccacca catcgagttc tggtgcgggg acgccaccaa cgtctcccgc 180
cgcttctcgt ggggactagg catgcgattc tccgccaaat ccgatctctc caccggaaac 240
atggttcacg cctcctacct actcacctcc ggcgacctcc gattcctctt caccgctccc 300
tactctccct ctctctccgc cggcgaagct caaccgtccg ctacagcctc aatcccatcg 360
ttcgatcacg cttcttgccg ctccttcttc tcttcgcacg gactcggcgt gagagccgta 420
gcgatcgaag tcgaagacgc tgagtcagca ttctcaatca gcgtagcaaa cggcgccgtt 480
ccttcctccc ctcctaacgt cctcaacgga gccgttacga tcgcggaagt taaactatac 540
ggagacgtcg tcctccgtta cgttagttat tataacggaa ccgttagttt cctccccgga 600
tttgaatctg ttgacgatac gtcgtcgttt ccgctagatt acggtatacg ccgtctcgac 660
cacgcggtgg gaaacgtccc cgagctcggc ccagctttaa cttacctcgc ggggttcacc 720
ggcttccacc agttcgcgga gttcactgca gacgacgtgg gaacagccga gagcggattg 780
aactcggctg tgctagccaa caacgacgag atggttctgt tgccgataaa cgagccggtt 840
cacgggacga agaggaagag ccagatccag acgtttcttg agcacaacga aggagccggg 900
ctgcagcatt tggctctgat gagcgaagat atattcagga cgctgaggga gatgaggaag 960
aggagcggcg ttggagggtt cgacttcatg ccttctcctc cgcctactta ttacaagaat 1020
ctcaagaaaa gggttggaga tgtgcttagt gaggagcaga ttgaggagtg tgaggagttg 1080
gggattcttg tggatagaga tgatcagggg acgttgcttc agatctttac gaaaccactt 1140
ggtgacaggc cgacgatatt tatagagata atacagagag tgggatgcat gaagagagat 1200
gaggaaggga aggtttacca gagtggagga tgtggtgggt ttggtaaagg taacttctct 1260
gagctcttta agtctattga agagtatgag aagacacttg aagccaaaca gcttgtgggt 1320
tga 1323
<210> 61
<211> 1323
<212> DNA
<213> 人工序列-BnHPPD-02核苷酸序列(Artificial Sequence)
<400> 61
atgggacatg aaaacgcggc agtgtgtgaa actcaacaac atgatgacgc cgcctccccg 60
gggtttaaac ttgtcgggtt ctcaaaattt gttaggaaga atccaaagtc tgacaagttc 120
aaggtgaaac gcttccacca catcgagttc tggtgcgggg atgcaacaaa tgtttcacgc 180
cgcttttcat ggggactagg aatgaggttt agcgcaaaga gtgatctctc cactggtaat 240
atggttcatg cgagctactt gctcacatct ggagacctga gattcctctt caccgcacca 300
tattctccct ccctgtcagc tggtgaagct cagccttcag caactgcaag tatcccttcg 360
tttgatcatg catcgtgcag gtctttcttc agttcacatg gactcggcgt cagagcagta 420
gcgatcgaag tagaggatgc tgaatcagct ttctccatat ctgtcgccaa cggcgcggtg 480
ccttcttctc caccaaacgt cttgaatggt gctgttacca ttgctgaggt gaagctgtac 540
ggagatgttg ttttaagata tgtttcatat tacaatggga ccgtttcttt tcttcccggc 600
tttgaatctg ttgatgatac ttcttccttc cctcttgatt atgggatcag gcggctggac 660
cacgccgtcg gcaacgtgcc ggagttaggt cctgctttga catatcttgc cggcttcact 720
ggattccatc aatttgcaga gtttacggct gacgacgtgg ggaccgcgga gagtgggctg 780
aactcagcag tcttggccaa caatgatgaa atggtgctcc tccccatcaa tgaacctgtg 840
catggaacaa agagaaagtc gcagattcaa acatttttag agcacaatga gggagctggg 900
ctacagcacc tcgcattgat gtcagaagat atttttcgaa cgttgaggga gatgcggaaa 960
cgatctggtg taggcggctt tgacttcatg ccatcccctc cgccaaccta ctacaagaac 1020
ctgaagaaga gggttggaga cgtcctttcc gaggagcaaa ttgaagaatg tgaggagctt 1080
ggaattctgg tggacagaga tgatcaagga acactacttc agatcttcac aaaaccattg 1140
ggcgacaggc caacgatatt cattgagatc attcagcgcg ttggatgcat gaagcgtgat 1200
gaggaaggga aggtctacca gagtggcggt tgtggtggat ttggaaaagg aaatttttct 1260
gagctgttca aaagcataga agagtatgag aaaacattgg aggccaagca gcttgttggt 1320
tga 1323
<210> 62
<211> 440
<212> PRT
<213> 人工序列-BnHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 62
Met Gly His Glu Asn Ala Ala Val Cys Glu Thr Gln Gln His Asp Asp
1 5 10 15
Ala Ala Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe Val Arg
20 25 30
Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His His Ile
35 40 45
Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ser Arg Arg Phe Ser Trp
50 55 60
Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr Gly Asn
65 70 75 80
Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg Phe Leu
85 90 95
Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ala Gln Pro
100 105 110
Ser Ala Thr Ala Ser Ile Pro Ser Phe Asp His Ala Ser Cys Arg Ser
115 120 125
Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile Glu Val
130 135 140
Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly Ala Val
145 150 155 160
Pro Ser Ser Pro Pro Asn Val Leu Asn Gly Ala Val Thr Ile Ala Glu
165 170 175
Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Tyr Asn
180 185 190
Gly Thr Val Ser Phe Leu Pro Gly Phe Glu Ser Val Asp Asp Thr Ser
195 200 205
Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu Asp His Ala Val Gly
210 215 220
Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr Leu Ala Gly Phe Thr
225 230 235 240
Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp Asp Val Gly Thr Ala
245 250 255
Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Asn Asn Asp Glu Met Val
260 265 270
Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr Lys Arg Lys Ser Gln
275 280 285
Ile Gln Thr Phe Leu Glu His Asn Glu Gly Ala Gly Leu Gln His Leu
290 295 300
Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu Arg Glu Met Arg Lys
305 310 315 320
Arg Ser Gly Val Gly Gly Phe Asp Phe Met Pro Ser Pro Pro Pro Thr
325 330 335
Tyr Tyr Lys Asn Leu Lys Lys Arg Val Gly Asp Val Leu Ser Glu Glu
340 345 350
Gln Ile Glu Glu Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp
355 360 365
Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro Leu Gly Asp Arg Pro
370 375 380
Thr Ile Phe Ile Glu Ile Ile Gln Arg Val Gly Cys Met Lys Arg Asp
385 390 395 400
Glu Glu Gly Lys Val Tyr Gln Ser Gly Gly Cys Gly Gly Phe Gly Lys
405 410 415
Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr
420 425 430
Leu Glu Ala Lys Gln Leu Val Gly
435 440
<210> 63
<211> 1323
<212> DNA
<213> 人工序列-BnHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 63
atggggcacg aaaacgccgc cgtttgcgaa actcagcagc acgacgacgc tgcgtcgcct 60
ggattcaagc tcgtcggatt ctccaagttc gtgaggaaga atccaaagtc cgacaagttc 120
aaggtaaagc gcttccacca catcgagttc tggtgcgggg acgccaccaa cgtctcccgc 180
cgcttctcgt ggggactagg catgcgattc tccgccaaat ccgatctctc caccggaaac 240
atggttcacg cctcctacct actcacctcc ggcgacctcc gattcctctt caccgctccc 300
tactctccct ctctctccgc cggcgaagct caaccgtccg ctacagcctc aatcccatcg 360
ttcgatcacg cttcttgccg ctccttcttc tcttcgcacg gactcggcgt gagagccgta 420
gcgatcgaag tcgaagacgc tgagtcagca ttctcaatca gcgtagcaaa cggcgccgtt 480
ccttcctccc ctcctaacgt cctcaacgga gccgttacga tcgcggaagt taaactatac 540
ggagacgtcg tcctccgtta cgttagttat tataacggaa ccgttagttt cctccccgga 600
tttgaatctg ttgacgatac gtcgtcgttt ccgctagatt acggtatacg ccgtctcgac 660
cacgcggtgg gaaacgtccc cgagctcggc ccagctttaa cttacctcgc ggggttcacc 720
ggcttccacc agttcgcgga gttcactgca gacgacgtgg gaacagccga gagcggattg 780
aactcggctg tgctagccaa caacgacgag atggttctgt tgccgataaa cgagccggtt 840
cacgggacga agaggaagag ccagatccag acgtttcttg agcacaacga aggagccggg 900
ctgcagcatt tggctctgat gagcgaagat atattcagga cgctgaggga gatgaggaag 960
aggagcggcg ttggagggtt cgacttcatg ccttctcctc cgcctactta ttacaagaat 1020
ctcaagaaaa gggttggaga tgtgcttagt gaggagcaga ttgaggagtg tgaggagttg 1080
gggattcttg tggatagaga tgatcagggg acgttgcttc agatcgctac gaaaccactt 1140
ggtgacaggc cgacgatatt tatagagata atacagagag tgggatgcat gaagagagat 1200
gaggaaggga aggtttacca gagtggagga tgtggtgggt ttggtaaagg taacttctct 1260
gagctcttta agtctattga agagtatgag aagacacttg aagccaaaca gcttgtgggt 1320
tga 1323
<210> 64
<211> 1323
<212> DNA
<213> 人工序列-BnHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 64
atgggacatg aaaacgcggc agtgtgtgaa actcaacaac atgatgacgc cgcctccccg 60
gggtttaaac ttgtcgggtt ctcaaaattt gttaggaaga atccaaagtc tgacaagttc 120
aaggtgaaac gcttccacca catcgagttc tggtgcgggg atgcaacaaa tgtttcacgc 180
cgcttttcat ggggactagg aatgaggttt agcgcaaaga gtgatctctc cactggtaat 240
atggttcatg cgagctactt gctcacatct ggagacctga gattcctctt caccgcacca 300
tattctccct ccctgtcagc tggtgaagct cagccttcag caactgcaag tatcccttcg 360
tttgatcatg catcgtgcag gtctttcttc agttcacatg gactcggcgt cagagcagta 420
gcgatcgaag tagaggatgc tgaatcagct ttctccatat ctgtcgccaa cggcgcggtg 480
ccttcttctc caccaaacgt cttgaatggt gctgttacca ttgctgaggt gaagctgtac 540
ggagatgttg ttttaagata tgtttcatat tacaatggga ccgtttcttt tcttcccggc 600
tttgaatctg ttgatgatac ttcttccttc cctcttgatt atgggatcag gcggctggac 660
cacgccgtcg gcaacgtgcc ggagttaggt cctgctttga catatcttgc cggcttcact 720
ggattccatc aatttgcaga gtttacggct gacgacgtgg ggaccgcgga gagtgggctg 780
aactcagcag tcttggccaa caatgatgaa atggtgctcc tccccatcaa tgaacctgtg 840
catggaacaa agagaaagtc gcagattcaa acatttttag agcacaatga gggagctggg 900
ctacagcacc tcgcattgat gtcagaagat atttttcgaa cgttgaggga gatgcggaaa 960
cgatctggtg taggcggctt tgacttcatg ccatcccctc cgccaaccta ctacaagaac 1020
ctgaagaaga gggttggaga cgtcctttcc gaggagcaaa ttgaagaatg tgaggagctt 1080
ggaattctgg tggacagaga tgatcaagga acactacttc agatcgccac aaaaccattg 1140
ggcgacaggc caacgatatt cattgagatc attcagcgcg ttggatgcat gaagcgtgat 1200
gaggaaggga aggtctacca gagtggcggt tgtggtggat ttggaaaagg aaatttttct 1260
gagctgttca aaagcataga agagtatgag aaaacattgg aggccaagca gcttgttggt 1320
tga 1323
<210> 65
<211> 440
<212> PRT
<213> 人工序列-BnHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 65
Met Gly His Glu Asn Ala Ala Val Cys Glu Thr Gln Gln His Asp Asp
1 5 10 15
Ala Ala Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe Val Arg
20 25 30
Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His His Ile
35 40 45
Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ser Arg Arg Phe Ser Trp
50 55 60
Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr Gly Asn
65 70 75 80
Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg Phe Leu
85 90 95
Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ala Gln Pro
100 105 110
Ser Ala Thr Ala Ser Ile Pro Ser Phe Asp His Ala Ser Cys Arg Ser
115 120 125
Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile Glu Val
130 135 140
Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly Ala Val
145 150 155 160
Pro Ser Ser Pro Pro Asn Val Leu Asn Gly Ala Val Thr Ile Ala Glu
165 170 175
Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Tyr Asn
180 185 190
Gly Thr Val Ser Phe Leu Pro Gly Phe Glu Ser Val Asp Asp Thr Ser
195 200 205
Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu Asp His Ala Val Gly
210 215 220
Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr Leu Ala Gly Phe Thr
225 230 235 240
Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp Asp Val Gly Thr Ala
245 250 255
Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Asn Asn Asp Glu Met Val
260 265 270
Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr Lys Arg Lys Ser Gln
275 280 285
Ile Gln Thr Phe Leu Glu His Asn Glu Gly Ala Gly Leu Gln His Leu
290 295 300
Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu Arg Glu Met Arg Lys
305 310 315 320
Arg Ser Gly Val Gly Gly Phe Asp Phe Met Pro Ser Pro Pro Pro Thr
325 330 335
Tyr Tyr Lys Asn Leu Lys Lys Arg Val Gly Asp Val Leu Ser Glu Glu
340 345 350
Gln Ile Glu Glu Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp
355 360 365
Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro Leu Gly Asp Arg Pro
370 375 380
Thr Ile Trp Ile Glu Ile Ile Gln Arg Val Gly Cys Met Lys Arg Asp
385 390 395 400
Glu Glu Gly Lys Val Tyr Gln Ser Gly Gly Cys Gly Gly Phe Gly Lys
405 410 415
Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr
420 425 430
Leu Glu Ala Lys Gln Leu Val Gly
435 440
<210> 66
<211> 1323
<212> DNA
<213> 人工序列-BnHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 66
atggggcacg aaaacgccgc cgtttgcgaa actcagcagc acgacgacgc tgcgtcgcct 60
ggattcaagc tcgtcggatt ctccaagttc gtgaggaaga atccaaagtc cgacaagttc 120
aaggtaaagc gcttccacca catcgagttc tggtgcgggg acgccaccaa cgtctcccgc 180
cgcttctcgt ggggactagg catgcgattc tccgccaaat ccgatctctc caccggaaac 240
atggttcacg cctcctacct actcacctcc ggcgacctcc gattcctctt caccgctccc 300
tactctccct ctctctccgc cggcgaagct caaccgtccg ctacagcctc aatcccatcg 360
ttcgatcacg cttcttgccg ctccttcttc tcttcgcacg gactcggcgt gagagccgta 420
gcgatcgaag tcgaagacgc tgagtcagca ttctcaatca gcgtagcaaa cggcgccgtt 480
ccttcctccc ctcctaacgt cctcaacgga gccgttacga tcgcggaagt taaactatac 540
ggagacgtcg tcctccgtta cgttagttat tataacggaa ccgttagttt cctccccgga 600
tttgaatctg ttgacgatac gtcgtcgttt ccgctagatt acggtatacg ccgtctcgac 660
cacgcggtgg gaaacgtccc cgagctcggc ccagctttaa cttacctcgc ggggttcacc 720
ggcttccacc agttcgcgga gttcactgca gacgacgtgg gaacagccga gagcggattg 780
aactcggctg tgctagccaa caacgacgag atggttctgt tgccgataaa cgagccggtt 840
cacgggacga agaggaagag ccagatccag acgtttcttg agcacaacga aggagccggg 900
ctgcagcatt tggctctgat gagcgaagat atattcagga cgctgaggga gatgaggaag 960
aggagcggcg ttggagggtt cgacttcatg ccttctcctc cgcctactta ttacaagaat 1020
ctcaagaaaa gggttggaga tgtgcttagt gaggagcaga ttgaggagtg tgaggagttg 1080
gggattcttg tggatagaga tgatcagggg acgttgcttc agatctttac gaaaccactt 1140
ggtgacaggc cgacgatatg gatagagata atacagagag tgggatgcat gaagagagat 1200
gaggaaggga aggtttacca gagtggagga tgtggtgggt ttggtaaagg taacttctct 1260
gagctcttta agtctattga agagtatgag aagacacttg aagccaaaca gcttgtgggt 1320
tga 1323
<210> 67
<211> 1323
<212> DNA
<213> 人工序列-BnHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 67
atgggacatg aaaacgcggc agtgtgtgaa actcaacaac atgatgacgc cgcctccccg 60
gggtttaaac ttgtcgggtt ctcaaaattt gttaggaaga atccaaagtc tgacaagttc 120
aaggtgaaac gcttccacca catcgagttc tggtgcgggg atgcaacaaa tgtttcacgc 180
cgcttttcat ggggactagg aatgaggttt agcgcaaaga gtgatctctc cactggtaat 240
atggttcatg cgagctactt gctcacatct ggagacctga gattcctctt caccgcacca 300
tattctccct ccctgtcagc tggtgaagct cagccttcag caactgcaag tatcccttcg 360
tttgatcatg catcgtgcag gtctttcttc agttcacatg gactcggcgt cagagcagta 420
gcgatcgaag tagaggatgc tgaatcagct ttctccatat ctgtcgccaa cggcgcggtg 480
ccttcttctc caccaaacgt cttgaatggt gctgttacca ttgctgaggt gaagctgtac 540
ggagatgttg ttttaagata tgtttcatat tacaatggga ccgtttcttt tcttcccggc 600
tttgaatctg ttgatgatac ttcttccttc cctcttgatt atgggatcag gcggctggac 660
cacgccgtcg gcaacgtgcc ggagttaggt cctgctttga catatcttgc cggcttcact 720
ggattccatc aatttgcaga gtttacggct gacgacgtgg ggaccgcgga gagtgggctg 780
aactcagcag tcttggccaa caatgatgaa atggtgctcc tccccatcaa tgaacctgtg 840
catggaacaa agagaaagtc gcagattcaa acatttttag agcacaatga gggagctggg 900
ctacagcacc tcgcattgat gtcagaagat atttttcgaa cgttgaggga gatgcggaaa 960
cgatctggtg taggcggctt tgacttcatg ccatcccctc cgccaaccta ctacaagaac 1020
ctgaagaaga gggttggaga cgtcctttcc gaggagcaaa ttgaagaatg tgaggagctt 1080
ggaattctgg tggacagaga tgatcaagga acactacttc agatcttcac aaaaccattg 1140
ggcgacaggc caacgatatg gattgagatc attcagcgcg ttggatgcat gaagcgtgat 1200
gaggaaggga aggtctacca gagtggcggt tgtggtggat ttggaaaagg aaatttttct 1260
gagctgttca aaagcataga agagtatgag aaaacattgg aggccaagca gcttgttggt 1320
tga 1323
<210> 68
<211> 440
<212> PRT
<213> 人工序列-BnHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 68
Met Gly His Glu Asn Ala Ala Val Cys Glu Thr Gln Gln His Asp Asp
1 5 10 15
Ala Ala Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe Val Arg
20 25 30
Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His His Ile
35 40 45
Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ser Arg Arg Phe Ser Trp
50 55 60
Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr Gly Asn
65 70 75 80
Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg Phe Leu
85 90 95
Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ala Gln Pro
100 105 110
Ser Ala Thr Ala Ser Ile Pro Ser Phe Asp His Ala Ser Cys Arg Ser
115 120 125
Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile Glu Val
130 135 140
Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly Ala Val
145 150 155 160
Pro Ser Ser Pro Pro Asn Val Leu Asn Gly Ala Val Thr Ile Ala Glu
165 170 175
Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Tyr Asn
180 185 190
Gly Thr Val Ser Phe Leu Pro Gly Phe Glu Ser Val Asp Asp Thr Ser
195 200 205
Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu Asp His Ala Val Gly
210 215 220
Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr Leu Ala Gly Phe Thr
225 230 235 240
Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp Asp Val Gly Thr Ala
245 250 255
Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Asn Asn Asp Glu Met Val
260 265 270
Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr Lys Arg Lys Ser Gln
275 280 285
Ile Gln Thr Phe Leu Glu His Asn Glu Gly Ala Gly Leu Gln His Leu
290 295 300
Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu Arg Glu Met Arg Lys
305 310 315 320
Arg Ser Gly Val Gly Gly Phe Asp Phe Met Pro Ser Pro Pro Pro Thr
325 330 335
Tyr Tyr Lys Asn Leu Lys Lys Arg Val Gly Asp Val Leu Ser Glu Glu
340 345 350
Gln Ile Glu Glu Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp Asp
355 360 365
Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro Leu Gly Asp Arg Pro
370 375 380
Thr Ile Trp Ile Glu Ile Ile Gln Arg Val Gly Cys Met Lys Arg Asp
385 390 395 400
Glu Glu Gly Lys Val Tyr Gln Ser Gly Gly Cys Gly Gly Phe Gly Lys
405 410 415
Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr
420 425 430
Leu Glu Ala Lys Gln Leu Val Gly
435 440
<210> 69
<211> 1323
<212> DNA
<213> 人工序列-BnHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 69
atggggcacg aaaacgccgc cgtttgcgaa actcagcagc acgacgacgc tgcgtcgcct 60
ggattcaagc tcgtcggatt ctccaagttc gtgaggaaga atccaaagtc cgacaagttc 120
aaggtaaagc gcttccacca catcgagttc tggtgcgggg acgccaccaa cgtctcccgc 180
cgcttctcgt ggggactagg catgcgattc tccgccaaat ccgatctctc caccggaaac 240
atggttcacg cctcctacct actcacctcc ggcgacctcc gattcctctt caccgctccc 300
tactctccct ctctctccgc cggcgaagct caaccgtccg ctacagcctc aatcccatcg 360
ttcgatcacg cttcttgccg ctccttcttc tcttcgcacg gactcggcgt gagagccgta 420
gcgatcgaag tcgaagacgc tgagtcagca ttctcaatca gcgtagcaaa cggcgccgtt 480
ccttcctccc ctcctaacgt cctcaacgga gccgttacga tcgcggaagt taaactatac 540
ggagacgtcg tcctccgtta cgttagttat tataacggaa ccgttagttt cctccccgga 600
tttgaatctg ttgacgatac gtcgtcgttt ccgctagatt acggtatacg ccgtctcgac 660
cacgcggtgg gaaacgtccc cgagctcggc ccagctttaa cttacctcgc ggggttcacc 720
ggcttccacc agttcgcgga gttcactgca gacgacgtgg gaacagccga gagcggattg 780
aactcggctg tgctagccaa caacgacgag atggttctgt tgccgataaa cgagccggtt 840
cacgggacga agaggaagag ccagatccag acgtttcttg agcacaacga aggagccggg 900
ctgcagcatt tggctctgat gagcgaagat atattcagga cgctgaggga gatgaggaag 960
aggagcggcg ttggagggtt cgacttcatg ccttctcctc cgcctactta ttacaagaat 1020
ctcaagaaaa gggttggaga tgtgcttagt gaggagcaga ttgaggagtg tgaggagttg 1080
gggattcttg tggatagaga tgatcagggg acgttgcttc agatcgctac gaaaccactt 1140
ggtgacaggc cgacgatatg gatagagata atacagagag tgggatgcat gaagagagat 1200
gaggaaggga aggtttacca gagtggagga tgtggtgggt ttggtaaagg taacttctct 1260
gagctcttta agtctattga agagtatgag aagacacttg aagccaaaca gcttgtgggt 1320
tga 1323
<210> 70
<211> 1323
<212> DNA
<213> 人工序列-BnHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 70
atgggacatg aaaacgcggc agtgtgtgaa actcaacaac atgatgacgc cgcctccccg 60
gggtttaaac ttgtcgggtt ctcaaaattt gttaggaaga atccaaagtc tgacaagttc 120
aaggtgaaac gcttccacca catcgagttc tggtgcgggg atgcaacaaa tgtttcacgc 180
cgcttttcat ggggactagg aatgaggttt agcgcaaaga gtgatctctc cactggtaat 240
atggttcatg cgagctactt gctcacatct ggagacctga gattcctctt caccgcacca 300
tattctccct ccctgtcagc tggtgaagct cagccttcag caactgcaag tatcccttcg 360
tttgatcatg catcgtgcag gtctttcttc agttcacatg gactcggcgt cagagcagta 420
gcgatcgaag tagaggatgc tgaatcagct ttctccatat ctgtcgccaa cggcgcggtg 480
ccttcttctc caccaaacgt cttgaatggt gctgttacca ttgctgaggt gaagctgtac 540
ggagatgttg ttttaagata tgtttcatat tacaatggga ccgtttcttt tcttcccggc 600
tttgaatctg ttgatgatac ttcttccttc cctcttgatt atgggatcag gcggctggac 660
cacgccgtcg gcaacgtgcc ggagttaggt cctgctttga catatcttgc cggcttcact 720
ggattccatc aatttgcaga gtttacggct gacgacgtgg ggaccgcgga gagtgggctg 780
aactcagcag tcttggccaa caatgatgaa atggtgctcc tccccatcaa tgaacctgtg 840
catggaacaa agagaaagtc gcagattcaa acatttttag agcacaatga gggagctggg 900
ctacagcacc tcgcattgat gtcagaagat atttttcgaa cgttgaggga gatgcggaaa 960
cgatctggtg taggcggctt tgacttcatg ccatcccctc cgccaaccta ctacaagaac 1020
ctgaagaaga gggttggaga cgtcctttcc gaggagcaaa ttgaagaatg tgaggagctt 1080
ggaattctgg tggacagaga tgatcaagga acactacttc agatcgccac aaaaccattg 1140
ggcgacaggc caacgatatg gattgagatc attcagcgcg ttggatgcat gaagcgtgat 1200
gaggaaggga aggtctacca gagtggcggt tgtggtggat ttggaaaagg aaatttttct 1260
gagctgttca aaagcataga agagtatgag aaaacattgg aggccaagca gcttgttggt 1320
tga 1323
<210> 71
<211> 488
<212> PRT
<213> 大豆野生型HPPD(GmHPPD的氨基酸序列Glycine max)
<400> 71
Met Pro Met Tyr Thr Pro Ser Leu Ser Ala Pro Ser Ser Asn His Ile
1 5 10 15
Gln Pro Ser Val Thr Leu Pro Leu Tyr Ile Thr Thr Thr Lys Leu Asn
20 25 30
Leu Lys Gln Gln His His Thr Thr Pro Met Pro Ile Pro Met Cys Asn
35 40 45
Glu Ile Gln Ala Gln Ala Gln Ala Gln Ala Gln Pro Gly Phe Lys Leu
50 55 60
Val Gly Phe Lys Asn Phe Val Arg Thr Asn Pro Lys Ser Asp Arg Phe
65 70 75 80
Gln Val Asn Arg Phe His His Ile Glu Phe Trp Cys Thr Asp Ala Thr
85 90 95
Asn Ala Ser Arg Arg Phe Ser Trp Gly Leu Gly Met Pro Ile Val Ala
100 105 110
Lys Ser Asp Leu Ser Thr Gly Asn Gln Ile His Ala Ser Tyr Leu Leu
115 120 125
Arg Ser Gly Asp Leu Ser Phe Leu Phe Ser Ala Pro Tyr Ser Pro Ser
130 135 140
Leu Ser Ala Gly Ser Ser Ala Ala Ser Ser Ala Ser Ile Pro Ser Phe
145 150 155 160
Asp Ala Ala Thr Cys Leu Ala Phe Ala Ala Lys His Gly Phe Gly Val
165 170 175
Arg Ala Ile Ala Leu Glu Val Ala Asp Ala Glu Ala Ala Phe Ser Ala
180 185 190
Ser Val Ala Lys Gly Ala Glu Pro Ala Ser Pro Pro Val Leu Val Asp
195 200 205
Asp Arg Thr Gly Phe Ala Glu Val Arg Leu Tyr Gly Asp Val Val Leu
210 215 220
Arg Tyr Val Ser Tyr Lys Asp Ala Ala Pro Gln Ala Pro His Ala Asp
225 230 235 240
Pro Ser Arg Trp Phe Leu Pro Gly Phe Glu Ala Ala Ala Ser Ser Ser
245 250 255
Ser Phe Pro Glu Leu Asp Tyr Gly Ile Arg Arg Leu Asp His Ala Val
260 265 270
Gly Asn Val Pro Glu Leu Ala Pro Ala Val Arg Tyr Leu Lys Gly Phe
275 280 285
Ser Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr
290 295 300
Ser Glu Ser Gly Leu Asn Ser Val Val Leu Ala Asn Asn Ser Glu Thr
305 310 315 320
Val Leu Leu Pro Leu Asn Glu Pro Val Tyr Gly Thr Lys Arg Lys Ser
325 330 335
Gln Ile Glu Thr Tyr Leu Glu His Asn Glu Gly Ala Gly Val Gln His
340 345 350
Leu Ala Leu Val Thr His Asp Ile Phe Thr Thr Leu Arg Glu Met Arg
355 360 365
Lys Arg Ser Phe Leu Gly Gly Phe Glu Phe Met Pro Ser Pro Pro Pro
370 375 380
Thr Tyr Tyr Ala Asn Leu His Asn Arg Ala Ala Asp Val Leu Thr Val
385 390 395 400
Asp Gln Ile Lys Gln Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp
405 410 415
Asp Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro Val Gly Asp Arg
420 425 430
Pro Thr Ile Phe Ile Glu Ile Ile Gln Arg Ile Gly Cys Met Val Glu
435 440 445
Asp Glu Glu Gly Lys Val Tyr Gln Lys Gly Ala Cys Gly Gly Phe Gly
450 455 460
Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys
465 470 475 480
Thr Leu Glu Ala Lys Arg Thr Ala
485
<210> 72
<211> 1467
<212> DNA
<213> GmHPPD-01核苷酸序列(Glycine max)
<400> 72
atgcccatgt acactccatc actctccgca ccctcctcca atcacattca accaagtgtc 60
acactcccct tatatatcac aaccaccaag ctcaatctca agcagcagca tcacaccaca 120
ccaatgccaa tacccatgtg caacgaaatt caagcccaag cccaagccca agcccaacct 180
gggtttaagc tcgtcggttt caaaaacttc gtccgaacca atcctaagtc ggaccgcttt 240
caagtcaacc gcttccacca catcgagttc tggtgcaccg atgccaccaa cgcctctcgc 300
cgattctctt ggggacttgg aatgcctatt gtggcaaaat ctgatctctc caccggaaac 360
caaatccacg cctcctacct cctccgctcc ggcgacctct ccttcctctt ctccgctcct 420
tactctccct ctctctccgc cggctcctcc gctgcctcct ccgcctccat tcccagtttc 480
gacgccgcca cctgccttgc cttcgctgcc aaacacggct tcggcgtccg cgccatcgcc 540
ttggaagtcg ccgacgcgga agccgctttc agcgccagcg tcgcgaaagg agccgagccg 600
gcgtcgccgc cggttctcgt cgacgatcgc accggcttcg cggaggtgcg cctctacggc 660
gacgtggtgc tccgctacgt cagctacaag gacgccgcgc cgcaggcgcc acacgcagat 720
ccgtcgcggt ggttcctgcc gggattcgag gccgcggcgt cgtcgtcttc gtttccggag 780
ctggactacg ggatccggcg gctggaccac gccgtcggga acgttccgga gctggcgccg 840
gcggtgaggt acctgaaagg cttcagcgga ttccacgagt tcgcggagtt caccgcggag 900
gacgtgggaa cgagcgagag cgggttgaac tcggtggttc tggcgaacaa ctcggagacg 960
gtgttgctgc cgctgaacga gccggtttac ggaacgaaga ggaagagcca gattgagacg 1020
tatttggaac acaacgaagg tgctggtgtg cagcaccttg cgcttgttac tcacgacatc 1080
ttcaccacac tgagagagat gagaaagcga agtttccttg gtggatttga gttcatgcct 1140
tctcctcctc ccacctatta cgccaacctc cacaaccgtg ccgctgatgt gttgaccgtt 1200
gaccagatta agcagtgtga ggagcttggg attcttgttg acagagatga tcagggcact 1260
ctgcttcaga ttttcacyaa gcctgttggg gacaggccaa cgatattcat agagataatt 1320
cagaggatcg ggtgcatggt ggaggatgag gaagggaagg tgtaccagaa gggtgcatgt 1380
gggggttttg ggaaaggcaa tttttctgag cttttcaaat ccattgaaga atatgagaag 1440
actttggaag ctaaaagaac cgcgtaa 1467
<210> 73
<211> 1467
<212> DNA
<213> 人工序列-GmHPPD-02核苷酸序列(Artificial Sequence)
<400> 73
atgccaatgt acacaccatc gctctccgcc ccttcttcca accatattca gccatccgtg 60
actctgcctc tctacatcac aaccaccaag ttgaacctga agcagcagca ccatacgact 120
cctatgccta tacccatgtg caatgagatc caagcacagg ctcaggcaca agctcaaccc 180
ggattcaaac tcgtgggatt caagaacttc gtccgtacca atccaaaatc tgaccgcttc 240
caggtgaaca gattccacca catagagttt tggtgtactg atgcaacgaa tgcttcgagg 300
aggttttcat ggggcttagg catgccgatt gtagcaaagt cagacctttc tacagggaac 360
caaattcatg caagctatct gctccgctca ggtgatttgt ctttcttgtt ctcagctcca 420
tattctccat cattatctgc tgggtcatca gcagcatcta gtgcgtcgat ccctagcttt 480
gatgccgcca cctgcctcgc cttcgccgcc aaacatggat ttggtgttcg cgccattgct 540
cttgaggttg ccgatgccga ggctgctttt agcgcctccg tcgccaaggg agctgagcct 600
gcgtcacctc ctgttcttgt tgatgatcga actggttttg ctgaagtgcg tctttacggc 660
gacgtggtgc tcagatatgt tagttacaag gatgctgctc ctcaagcgcc gcacgctgat 720
ccttcaagat ggtttcttcc ggggtttgag gccgctgcta gttcatcttc cttcccggag 780
ttggattacg gcatacggcg gcttgaccat gctgtcggca atgttcctga gcttgctccg 840
gcggtacggt acttgaaagg tttctccggg ttccatgaat ttgcagagtt cacggcggaa 900
gatgttggaa caagtgaaag cggattgaat tctgttgttt tggccaacaa cagtgaaact 960
gttctgctcc ccctcaatga accagtttat ggcacaaagc gcaagagcca gattgaaaca 1020
tatctcgagc acaatgaagg cgccggcgtc cagcatcttg ctttggtgac tcatgatatc 1080
ttcacaacac ttagggagat gaggaagagg agtttcctcg gcgggtttga attcatgccc 1140
agtcccccgc cgacctacta tgcaaatctg cacaacaggg ctgcagatgt gctgactgta 1200
gaccagatca agcaatgtga agaacttgga attttagtgg atagagacga ccaaggtaca 1260
ctacttcaaa tattcaccaa accagtcggt gacagaccaa caattttcat cgagattata 1320
caaagaattg gatgcatggt cgaggatgaa gaagggaagg tgtaccagaa gggagcatgt 1380
ggtggttttg ggaaaggaaa tttttctgag ctgtttaaat ccatcgagga atatgagaaa 1440
accttagaag ctaaaagaac tgcatga 1467
<210> 74
<211> 488
<212> PRT
<213> 人工序列-GmHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 74
Met Pro Met Tyr Thr Pro Ser Leu Ser Ala Pro Ser Ser Asn His Ile
1 5 10 15
Gln Pro Ser Val Thr Leu Pro Leu Tyr Ile Thr Thr Thr Lys Leu Asn
20 25 30
Leu Lys Gln Gln His His Thr Thr Pro Met Pro Ile Pro Met Cys Asn
35 40 45
Glu Ile Gln Ala Gln Ala Gln Ala Gln Ala Gln Pro Gly Phe Lys Leu
50 55 60
Val Gly Phe Lys Asn Phe Val Arg Thr Asn Pro Lys Ser Asp Arg Phe
65 70 75 80
Gln Val Asn Arg Phe His His Ile Glu Phe Trp Cys Thr Asp Ala Thr
85 90 95
Asn Ala Ser Arg Arg Phe Ser Trp Gly Leu Gly Met Pro Ile Val Ala
100 105 110
Lys Ser Asp Leu Ser Thr Gly Asn Gln Ile His Ala Ser Tyr Leu Leu
115 120 125
Arg Ser Gly Asp Leu Ser Phe Leu Phe Ser Ala Pro Tyr Ser Pro Ser
130 135 140
Leu Ser Ala Gly Ser Ser Ala Ala Ser Ser Ala Ser Ile Pro Ser Phe
145 150 155 160
Asp Ala Ala Thr Cys Leu Ala Phe Ala Ala Lys His Gly Phe Gly Val
165 170 175
Arg Ala Ile Ala Leu Glu Val Ala Asp Ala Glu Ala Ala Phe Ser Ala
180 185 190
Ser Val Ala Lys Gly Ala Glu Pro Ala Ser Pro Pro Val Leu Val Asp
195 200 205
Asp Arg Thr Gly Phe Ala Glu Val Arg Leu Tyr Gly Asp Val Val Leu
210 215 220
Arg Tyr Val Ser Tyr Lys Asp Ala Ala Pro Gln Ala Pro His Ala Asp
225 230 235 240
Pro Ser Arg Trp Phe Leu Pro Gly Phe Glu Ala Ala Ala Ser Ser Ser
245 250 255
Ser Phe Pro Glu Leu Asp Tyr Gly Ile Arg Arg Leu Asp His Ala Val
260 265 270
Gly Asn Val Pro Glu Leu Ala Pro Ala Val Arg Tyr Leu Lys Gly Phe
275 280 285
Ser Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr
290 295 300
Ser Glu Ser Gly Leu Asn Ser Val Val Leu Ala Asn Asn Ser Glu Thr
305 310 315 320
Val Leu Leu Pro Leu Asn Glu Pro Val Tyr Gly Thr Lys Arg Lys Ser
325 330 335
Gln Ile Glu Thr Tyr Leu Glu His Asn Glu Gly Ala Gly Val Gln His
340 345 350
Leu Ala Leu Val Thr His Asp Ile Phe Thr Thr Leu Arg Glu Met Arg
355 360 365
Lys Arg Ser Phe Leu Gly Gly Phe Glu Phe Met Pro Ser Pro Pro Pro
370 375 380
Thr Tyr Tyr Ala Asn Leu His Asn Arg Ala Ala Asp Val Leu Thr Val
385 390 395 400
Asp Gln Ile Lys Gln Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp
405 410 415
Asp Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg
420 425 430
Pro Thr Ile Phe Ile Glu Ile Ile Gln Arg Ile Gly Cys Met Val Glu
435 440 445
Asp Glu Glu Gly Lys Val Tyr Gln Lys Gly Ala Cys Gly Gly Phe Gly
450 455 460
Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys
465 470 475 480
Thr Leu Glu Ala Lys Arg Thr Ala
485
<210> 75
<211> 1467
<212> DNA
<213> 人工序列-GmHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 75
atgcccatgt acactccatc actctccgca ccctcctcca atcacattca accaagtgtc 60
acactcccct tatatatcac aaccaccaag ctcaatctca agcagcagca tcacaccaca 120
ccaatgccaa tacccatgtg caacgaaatt caagcccaag cccaagccca agcccaacct 180
gggtttaagc tcgtcggttt caaaaacttc gtccgaacca atcctaagtc ggaccgcttt 240
caagtcaacc gcttccacca catcgagttc tggtgcaccg atgccaccaa cgcctctcgc 300
cgattctctt ggggacttgg aatgcctatt gtggcaaaat ctgatctctc caccggaaac 360
caaatccacg cctcctacct cctccgctcc ggcgacctct ccttcctctt ctccgctcct 420
tactctccct ctctctccgc cggctcctcc gctgcctcct ccgcctccat tcccagtttc 480
gacgccgcca cctgccttgc cttcgctgcc aaacacggct tcggcgtccg cgccatcgcc 540
ttggaagtcg ccgacgcgga agccgctttc agcgccagcg tcgcgaaagg agccgagccg 600
gcgtcgccgc cggttctcgt cgacgatcgc accggcttcg cggaggtgcg cctctacggc 660
gacgtggtgc tccgctacgt cagctacaag gacgccgcgc cgcaggcgcc acacgcagat 720
ccgtcgcggt ggttcctgcc gggattcgag gccgcggcgt cgtcgtcttc gtttccggag 780
ctggactacg ggatccggcg gctggaccac gccgtcggga acgttccgga gctggcgccg 840
gcggtgaggt acctgaaagg cttcagcgga ttccacgagt tcgcggagtt caccgcggag 900
gacgtgggaa cgagcgagag cgggttgaac tcggtggttc tggcgaacaa ctcggagacg 960
gtgttgctgc cgctgaacga gccggtttac ggaacgaaga ggaagagcca gattgagacg 1020
tatttggaac acaacgaagg tgctggtgtg cagcaccttg cgcttgttac tcacgacatc 1080
ttcaccacac tgagagagat gagaaagcga agtttccttg gtggatttga gttcatgcct 1140
tctcctcctc ccacctatta cgccaacctc cacaaccgtg ccgctgatgt gttgaccgtt 1200
gaccagatta agcagtgtga ggagcttggg attcttgttg acagagatga tcagggcact 1260
ctgcttcaga ttgctacyaa gcctgttggg gacaggccaa cgatattcat agagataatt 1320
cagaggatcg ggtgcatggt ggaggatgag gaagggaagg tgtaccagaa gggtgcatgt 1380
gggggttttg ggaaaggcaa tttttctgag cttttcaaat ccattgaaga atatgagaag 1440
actttggaag ctaaaagaac cgcgtaa 1467
<210> 76
<211> 1467
<212> DNA
<213> 人工序列-GmHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 76
atgccaatgt acacaccatc gctctccgcc ccttcttcca accatattca gccatccgtg 60
actctgcctc tctacatcac aaccaccaag ttgaacctga agcagcagca ccatacgact 120
cctatgccta tacccatgtg caatgagatc caagcacagg ctcaggcaca agctcaaccc 180
ggattcaaac tcgtgggatt caagaacttc gtccgtacca atccaaaatc tgaccgcttc 240
caggtgaaca gattccacca catagagttt tggtgtactg atgcaacgaa tgcttcgagg 300
aggttttcat ggggcttagg catgccgatt gtagcaaagt cagacctttc tacagggaac 360
caaattcatg caagctatct gctccgctca ggtgatttgt ctttcttgtt ctcagctcca 420
tattctccat cattatctgc tgggtcatca gcagcatcta gtgcgtcgat ccctagcttt 480
gatgccgcca cctgcctcgc cttcgccgcc aaacatggat ttggtgttcg cgccattgct 540
cttgaggttg ccgatgccga ggctgctttt agcgcctccg tcgccaaggg agctgagcct 600
gcgtcacctc ctgttcttgt tgatgatcga actggttttg ctgaagtgcg tctttacggc 660
gacgtggtgc tcagatatgt tagttacaag gatgctgctc ctcaagcgcc gcacgctgat 720
ccttcaagat ggtttcttcc ggggtttgag gccgctgcta gttcatcttc cttcccggag 780
ttggattacg gcatacggcg gcttgaccat gctgtcggca atgttcctga gcttgctccg 840
gcggtacggt acttgaaagg tttctccggg ttccatgaat ttgcagagtt cacggcggaa 900
gatgttggaa caagtgaaag cggattgaat tctgttgttt tggccaacaa cagtgaaact 960
gttctgctcc ccctcaatga accagtttat ggcacaaagc gcaagagcca gattgaaaca 1020
tatctcgagc acaatgaagg cgccggcgtc cagcatcttg ctttggtgac tcatgatatc 1080
ttcacaacac ttagggagat gaggaagagg agtttcctcg gcgggtttga attcatgccc 1140
agtcccccgc cgacctacta tgcaaatctg cacaacaggg ctgcagatgt gctgactgta 1200
gaccagatca agcaatgtga agaacttgga attttagtgg atagagacga ccaaggtaca 1260
ctacttcaaa tagccaccaa accagtcggt gacagaccaa caattttcat cgagattata 1320
caaagaattg gatgcatggt cgaggatgaa gaagggaagg tgtaccagaa gggagcatgt 1380
ggtggttttg ggaaaggaaa tttttctgag ctgtttaaat ccatcgagga atatgagaaa 1440
accttagaag ctaaaagaac tgcatga 1467
<210> 77
<211> 488
<212> PRT
<213> 人工序列-GmHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 77
Met Pro Met Tyr Thr Pro Ser Leu Ser Ala Pro Ser Ser Asn His Ile
1 5 10 15
Gln Pro Ser Val Thr Leu Pro Leu Tyr Ile Thr Thr Thr Lys Leu Asn
20 25 30
Leu Lys Gln Gln His His Thr Thr Pro Met Pro Ile Pro Met Cys Asn
35 40 45
Glu Ile Gln Ala Gln Ala Gln Ala Gln Ala Gln Pro Gly Phe Lys Leu
50 55 60
Val Gly Phe Lys Asn Phe Val Arg Thr Asn Pro Lys Ser Asp Arg Phe
65 70 75 80
Gln Val Asn Arg Phe His His Ile Glu Phe Trp Cys Thr Asp Ala Thr
85 90 95
Asn Ala Ser Arg Arg Phe Ser Trp Gly Leu Gly Met Pro Ile Val Ala
100 105 110
Lys Ser Asp Leu Ser Thr Gly Asn Gln Ile His Ala Ser Tyr Leu Leu
115 120 125
Arg Ser Gly Asp Leu Ser Phe Leu Phe Ser Ala Pro Tyr Ser Pro Ser
130 135 140
Leu Ser Ala Gly Ser Ser Ala Ala Ser Ser Ala Ser Ile Pro Ser Phe
145 150 155 160
Asp Ala Ala Thr Cys Leu Ala Phe Ala Ala Lys His Gly Phe Gly Val
165 170 175
Arg Ala Ile Ala Leu Glu Val Ala Asp Ala Glu Ala Ala Phe Ser Ala
180 185 190
Ser Val Ala Lys Gly Ala Glu Pro Ala Ser Pro Pro Val Leu Val Asp
195 200 205
Asp Arg Thr Gly Phe Ala Glu Val Arg Leu Tyr Gly Asp Val Val Leu
210 215 220
Arg Tyr Val Ser Tyr Lys Asp Ala Ala Pro Gln Ala Pro His Ala Asp
225 230 235 240
Pro Ser Arg Trp Phe Leu Pro Gly Phe Glu Ala Ala Ala Ser Ser Ser
245 250 255
Ser Phe Pro Glu Leu Asp Tyr Gly Ile Arg Arg Leu Asp His Ala Val
260 265 270
Gly Asn Val Pro Glu Leu Ala Pro Ala Val Arg Tyr Leu Lys Gly Phe
275 280 285
Ser Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr
290 295 300
Ser Glu Ser Gly Leu Asn Ser Val Val Leu Ala Asn Asn Ser Glu Thr
305 310 315 320
Val Leu Leu Pro Leu Asn Glu Pro Val Tyr Gly Thr Lys Arg Lys Ser
325 330 335
Gln Ile Glu Thr Tyr Leu Glu His Asn Glu Gly Ala Gly Val Gln His
340 345 350
Leu Ala Leu Val Thr His Asp Ile Phe Thr Thr Leu Arg Glu Met Arg
355 360 365
Lys Arg Ser Phe Leu Gly Gly Phe Glu Phe Met Pro Ser Pro Pro Pro
370 375 380
Thr Tyr Tyr Ala Asn Leu His Asn Arg Ala Ala Asp Val Leu Thr Val
385 390 395 400
Asp Gln Ile Lys Gln Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp
405 410 415
Asp Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro Val Gly Asp Arg
420 425 430
Pro Thr Ile Trp Ile Glu Ile Ile Gln Arg Ile Gly Cys Met Val Glu
435 440 445
Asp Glu Glu Gly Lys Val Tyr Gln Lys Gly Ala Cys Gly Gly Phe Gly
450 455 460
Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys
465 470 475 480
Thr Leu Glu Ala Lys Arg Thr Ala
485
<210> 78
<211> 1467
<212> DNA
<213> 人工序列-GmHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 78
atgcccatgt acactccatc actctccgca ccctcctcca atcacattca accaagtgtc 60
acactcccct tatatatcac aaccaccaag ctcaatctca agcagcagca tcacaccaca 120
ccaatgccaa tacccatgtg caacgaaatt caagcccaag cccaagccca agcccaacct 180
gggtttaagc tcgtcggttt caaaaacttc gtccgaacca atcctaagtc ggaccgcttt 240
caagtcaacc gcttccacca catcgagttc tggtgcaccg atgccaccaa cgcctctcgc 300
cgattctctt ggggacttgg aatgcctatt gtggcaaaat ctgatctctc caccggaaac 360
caaatccacg cctcctacct cctccgctcc ggcgacctct ccttcctctt ctccgctcct 420
tactctccct ctctctccgc cggctcctcc gctgcctcct ccgcctccat tcccagtttc 480
gacgccgcca cctgccttgc cttcgctgcc aaacacggct tcggcgtccg cgccatcgcc 540
ttggaagtcg ccgacgcgga agccgctttc agcgccagcg tcgcgaaagg agccgagccg 600
gcgtcgccgc cggttctcgt cgacgatcgc accggcttcg cggaggtgcg cctctacggc 660
gacgtggtgc tccgctacgt cagctacaag gacgccgcgc cgcaggcgcc acacgcagat 720
ccgtcgcggt ggttcctgcc gggattcgag gccgcggcgt cgtcgtcttc gtttccggag 780
ctggactacg ggatccggcg gctggaccac gccgtcggga acgttccgga gctggcgccg 840
gcggtgaggt acctgaaagg cttcagcgga ttccacgagt tcgcggagtt caccgcggag 900
gacgtgggaa cgagcgagag cgggttgaac tcggtggttc tggcgaacaa ctcggagacg 960
gtgttgctgc cgctgaacga gccggtttac ggaacgaaga ggaagagcca gattgagacg 1020
tatttggaac acaacgaagg tgctggtgtg cagcaccttg cgcttgttac tcacgacatc 1080
ttcaccacac tgagagagat gagaaagcga agtttccttg gtggatttga gttcatgcct 1140
tctcctcctc ccacctatta cgccaacctc cacaaccgtg ccgctgatgt gttgaccgtt 1200
gaccagatta agcagtgtga ggagcttggg attcttgttg acagagatga tcagggcact 1260
ctgcttcaga ttttcacyaa gcctgttggg gacaggccaa cgatatggat agagataatt 1320
cagaggatcg ggtgcatggt ggaggatgag gaagggaagg tgtaccagaa gggtgcatgt 1380
gggggttttg ggaaaggcaa tttttctgag cttttcaaat ccattgaaga atatgagaag 1440
actttggaag ctaaaagaac cgcgtaa 1467
<210> 79
<211> 1467
<212> DNA
<213> 人工序列-GmHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 79
atgccaatgt acacaccatc gctctccgcc ccttcttcca accatattca gccatccgtg 60
actctgcctc tctacatcac aaccaccaag ttgaacctga agcagcagca ccatacgact 120
cctatgccta tacccatgtg caatgagatc caagcacagg ctcaggcaca agctcaaccc 180
ggattcaaac tcgtgggatt caagaacttc gtccgtacca atccaaaatc tgaccgcttc 240
caggtgaaca gattccacca catagagttt tggtgtactg atgcaacgaa tgcttcgagg 300
aggttttcat ggggcttagg catgccgatt gtagcaaagt cagacctttc tacagggaac 360
caaattcatg caagctatct gctccgctca ggtgatttgt ctttcttgtt ctcagctcca 420
tattctccat cattatctgc tgggtcatca gcagcatcta gtgcgtcgat ccctagcttt 480
gatgccgcca cctgcctcgc cttcgccgcc aaacatggat ttggtgttcg cgccattgct 540
cttgaggttg ccgatgccga ggctgctttt agcgcctccg tcgccaaggg agctgagcct 600
gcgtcacctc ctgttcttgt tgatgatcga actggttttg ctgaagtgcg tctttacggc 660
gacgtggtgc tcagatatgt tagttacaag gatgctgctc ctcaagcgcc gcacgctgat 720
ccttcaagat ggtttcttcc ggggtttgag gccgctgcta gttcatcttc cttcccggag 780
ttggattacg gcatacggcg gcttgaccat gctgtcggca atgttcctga gcttgctccg 840
gcggtacggt acttgaaagg tttctccggg ttccatgaat ttgcagagtt cacggcggaa 900
gatgttggaa caagtgaaag cggattgaat tctgttgttt tggccaacaa cagtgaaact 960
gttctgctcc ccctcaatga accagtttat ggcacaaagc gcaagagcca gattgaaaca 1020
tatctcgagc acaatgaagg cgccggcgtc cagcatcttg ctttggtgac tcatgatatc 1080
ttcacaacac ttagggagat gaggaagagg agtttcctcg gcgggtttga attcatgccc 1140
agtcccccgc cgacctacta tgcaaatctg cacaacaggg ctgcagatgt gctgactgta 1200
gaccagatca agcaatgtga agaacttgga attttagtgg atagagacga ccaaggtaca 1260
ctacttcaaa tattcaccaa accagtcggt gacagaccaa caatttggat cgagattata 1320
caaagaattg gatgcatggt cgaggatgaa gaagggaagg tgtaccagaa gggagcatgt 1380
ggtggttttg ggaaaggaaa tttttctgag ctgtttaaat ccatcgagga atatgagaaa 1440
accttagaag ctaaaagaac tgcatga 1467
<210> 80
<211> 488
<212> PRT
<213> 人工序列-GmHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 80
Met Pro Met Tyr Thr Pro Ser Leu Ser Ala Pro Ser Ser Asn His Ile
1 5 10 15
Gln Pro Ser Val Thr Leu Pro Leu Tyr Ile Thr Thr Thr Lys Leu Asn
20 25 30
Leu Lys Gln Gln His His Thr Thr Pro Met Pro Ile Pro Met Cys Asn
35 40 45
Glu Ile Gln Ala Gln Ala Gln Ala Gln Ala Gln Pro Gly Phe Lys Leu
50 55 60
Val Gly Phe Lys Asn Phe Val Arg Thr Asn Pro Lys Ser Asp Arg Phe
65 70 75 80
Gln Val Asn Arg Phe His His Ile Glu Phe Trp Cys Thr Asp Ala Thr
85 90 95
Asn Ala Ser Arg Arg Phe Ser Trp Gly Leu Gly Met Pro Ile Val Ala
100 105 110
Lys Ser Asp Leu Ser Thr Gly Asn Gln Ile His Ala Ser Tyr Leu Leu
115 120 125
Arg Ser Gly Asp Leu Ser Phe Leu Phe Ser Ala Pro Tyr Ser Pro Ser
130 135 140
Leu Ser Ala Gly Ser Ser Ala Ala Ser Ser Ala Ser Ile Pro Ser Phe
145 150 155 160
Asp Ala Ala Thr Cys Leu Ala Phe Ala Ala Lys His Gly Phe Gly Val
165 170 175
Arg Ala Ile Ala Leu Glu Val Ala Asp Ala Glu Ala Ala Phe Ser Ala
180 185 190
Ser Val Ala Lys Gly Ala Glu Pro Ala Ser Pro Pro Val Leu Val Asp
195 200 205
Asp Arg Thr Gly Phe Ala Glu Val Arg Leu Tyr Gly Asp Val Val Leu
210 215 220
Arg Tyr Val Ser Tyr Lys Asp Ala Ala Pro Gln Ala Pro His Ala Asp
225 230 235 240
Pro Ser Arg Trp Phe Leu Pro Gly Phe Glu Ala Ala Ala Ser Ser Ser
245 250 255
Ser Phe Pro Glu Leu Asp Tyr Gly Ile Arg Arg Leu Asp His Ala Val
260 265 270
Gly Asn Val Pro Glu Leu Ala Pro Ala Val Arg Tyr Leu Lys Gly Phe
275 280 285
Ser Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr
290 295 300
Ser Glu Ser Gly Leu Asn Ser Val Val Leu Ala Asn Asn Ser Glu Thr
305 310 315 320
Val Leu Leu Pro Leu Asn Glu Pro Val Tyr Gly Thr Lys Arg Lys Ser
325 330 335
Gln Ile Glu Thr Tyr Leu Glu His Asn Glu Gly Ala Gly Val Gln His
340 345 350
Leu Ala Leu Val Thr His Asp Ile Phe Thr Thr Leu Arg Glu Met Arg
355 360 365
Lys Arg Ser Phe Leu Gly Gly Phe Glu Phe Met Pro Ser Pro Pro Pro
370 375 380
Thr Tyr Tyr Ala Asn Leu His Asn Arg Ala Ala Asp Val Leu Thr Val
385 390 395 400
Asp Gln Ile Lys Gln Cys Glu Glu Leu Gly Ile Leu Val Asp Arg Asp
405 410 415
Asp Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg
420 425 430
Pro Thr Ile Trp Ile Glu Ile Ile Gln Arg Ile Gly Cys Met Val Glu
435 440 445
Asp Glu Glu Gly Lys Val Tyr Gln Lys Gly Ala Cys Gly Gly Phe Gly
450 455 460
Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys
465 470 475 480
Thr Leu Glu Ala Lys Arg Thr Ala
485
<210> 81
<211> 1467
<212> DNA
<213> 人工序列-GmHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 81
atgcccatgt acactccatc actctccgca ccctcctcca atcacattca accaagtgtc 60
acactcccct tatatatcac aaccaccaag ctcaatctca agcagcagca tcacaccaca 120
ccaatgccaa tacccatgtg caacgaaatt caagcccaag cccaagccca agcccaacct 180
gggtttaagc tcgtcggttt caaaaacttc gtccgaacca atcctaagtc ggaccgcttt 240
caagtcaacc gcttccacca catcgagttc tggtgcaccg atgccaccaa cgcctctcgc 300
cgattctctt ggggacttgg aatgcctatt gtggcaaaat ctgatctctc caccggaaac 360
caaatccacg cctcctacct cctccgctcc ggcgacctct ccttcctctt ctccgctcct 420
tactctccct ctctctccgc cggctcctcc gctgcctcct ccgcctccat tcccagtttc 480
gacgccgcca cctgccttgc cttcgctgcc aaacacggct tcggcgtccg cgccatcgcc 540
ttggaagtcg ccgacgcgga agccgctttc agcgccagcg tcgcgaaagg agccgagccg 600
gcgtcgccgc cggttctcgt cgacgatcgc accggcttcg cggaggtgcg cctctacggc 660
gacgtggtgc tccgctacgt cagctacaag gacgccgcgc cgcaggcgcc acacgcagat 720
ccgtcgcggt ggttcctgcc gggattcgag gccgcggcgt cgtcgtcttc gtttccggag 780
ctggactacg ggatccggcg gctggaccac gccgtcggga acgttccgga gctggcgccg 840
gcggtgaggt acctgaaagg cttcagcgga ttccacgagt tcgcggagtt caccgcggag 900
gacgtgggaa cgagcgagag cgggttgaac tcggtggttc tggcgaacaa ctcggagacg 960
gtgttgctgc cgctgaacga gccggtttac ggaacgaaga ggaagagcca gattgagacg 1020
tatttggaac acaacgaagg tgctggtgtg cagcaccttg cgcttgttac tcacgacatc 1080
ttcaccacac tgagagagat gagaaagcga agtttccttg gtggatttga gttcatgcct 1140
tctcctcctc ccacctatta cgccaacctc cacaaccgtg ccgctgatgt gttgaccgtt 1200
gaccagatta agcagtgtga ggagcttggg attcttgttg acagagatga tcagggcact 1260
ctgcttcaga ttgctacyaa gcctgttggg gacaggccaa cgatatggat agagataatt 1320
cagaggatcg ggtgcatggt ggaggatgag gaagggaagg tgtaccagaa gggtgcatgt 1380
gggggttttg ggaaaggcaa tttttctgag cttttcaaat ccattgaaga atatgagaag 1440
actttggaag ctaaaagaac cgcgtaa 1467
<210> 82
<211> 1467
<212> DNA
<213> 人工序列-GmHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 82
atgccaatgt acacaccatc gctctccgcc ccttcttcca accatattca gccatccgtg 60
actctgcctc tctacatcac aaccaccaag ttgaacctga agcagcagca ccatacgact 120
cctatgccta tacccatgtg caatgagatc caagcacagg ctcaggcaca agctcaaccc 180
ggattcaaac tcgtgggatt caagaacttc gtccgtacca atccaaaatc tgaccgcttc 240
caggtgaaca gattccacca catagagttt tggtgtactg atgcaacgaa tgcttcgagg 300
aggttttcat ggggcttagg catgccgatt gtagcaaagt cagacctttc tacagggaac 360
caaattcatg caagctatct gctccgctca ggtgatttgt ctttcttgtt ctcagctcca 420
tattctccat cattatctgc tgggtcatca gcagcatcta gtgcgtcgat ccctagcttt 480
gatgccgcca cctgcctcgc cttcgccgcc aaacatggat ttggtgttcg cgccattgct 540
cttgaggttg ccgatgccga ggctgctttt agcgcctccg tcgccaaggg agctgagcct 600
gcgtcacctc ctgttcttgt tgatgatcga actggttttg ctgaagtgcg tctttacggc 660
gacgtggtgc tcagatatgt tagttacaag gatgctgctc ctcaagcgcc gcacgctgat 720
ccttcaagat ggtttcttcc ggggtttgag gccgctgcta gttcatcttc cttcccggag 780
ttggattacg gcatacggcg gcttgaccat gctgtcggca atgttcctga gcttgctccg 840
gcggtacggt acttgaaagg tttctccggg ttccatgaat ttgcagagtt cacggcggaa 900
gatgttggaa caagtgaaag cggattgaat tctgttgttt tggccaacaa cagtgaaact 960
gttctgctcc ccctcaatga accagtttat ggcacaaagc gcaagagcca gattgaaaca 1020
tatctcgagc acaatgaagg cgccggcgtc cagcatcttg ctttggtgac tcatgatatc 1080
ttcacaacac ttagggagat gaggaagagg agtttcctcg gcgggtttga attcatgccc 1140
agtcccccgc cgacctacta tgcaaatctg cacaacaggg ctgcagatgt gctgactgta 1200
gaccagatca agcaatgtga agaacttgga attttagtgg atagagacga ccaaggtaca 1260
ctacttcaaa tagccaccaa accagtcggt gacagaccaa caatttggat cgagattata 1320
caaagaattg gatgcatggt cgaggatgaa gaagggaagg tgtaccagaa gggagcatgt 1380
ggtggttttg ggaaaggaaa tttttctgag ctgtttaaat ccatcgagga atatgagaaa 1440
accttagaag ctaaaagaac tgcatga 1467
<210> 83
<211> 447
<212> PRT
<213> 烟草野生型HPPD(NtHPPD的氨基酸序列Nicotiana tabacum)
<400> 83
Met Gly Lys Leu Glu Thr Val Thr Thr Thr Ser Ala Ala Ala Ala Asp
1 5 10 15
Asp Ser Ser Glu Leu Thr Thr Asn Phe Lys Leu Val Gly Phe Lys Asn
20 25 30
Phe Ile Arg Thr Asn Pro Arg Ser Asp Phe Phe Ser Val Lys Ser Phe
35 40 45
His His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Thr Ala Arg Arg
50 55 60
Phe Ser Trp Ser Leu Gly Met Pro Ile Ala Ala Lys Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Ser Val His Ala Ser Tyr Leu Leu Arg Pro Val Ser Gly
85 90 95
Ser Leu Gln Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Ile Ser Thr
100 105 110
Pro Ser Ser Ala Ala Ile Pro Ser Phe Ser Thr Ser Thr His Arg Ser
115 120 125
Phe Thr Thr Thr His Gly Leu Gly Val Arg Ala Val Ala Leu Glu Val
130 135 140
Glu Asn Ala Tyr Thr Ala Phe Ser Ala Ser Val Ser Arg Gly Ala Lys
145 150 155 160
Pro Met Phe Glu Pro Val Thr Ile Asp Glu Gln Val Ala Met Ala Glu
165 170 175
Val His Leu Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Leu Lys
180 185 190
Asp Ala Asp Asn Leu Val Phe Leu Pro Gly Phe Glu Ala Met Asp Glu
195 200 205
Thr Ala Ser Tyr Lys Glu Leu Asp Tyr Gly Ile Arg Arg Leu Asp His
210 215 220
Ala Val Gly Asn Val Pro Glu Leu Gly Pro Val Val Asp Tyr Ile Lys
225 230 235 240
Arg Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val
245 250 255
Gly Thr Ala Glu Ser Gly Leu Asn Ser Met Val Leu Ala Asn Asn Asp
260 265 270
Glu Thr Val Leu Leu Pro Leu Asn Glu Pro Val Tyr Gly Thr Lys Arg
275 280 285
Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala Gly Val
290 295 300
Gln His Leu Ala Leu Val Thr Glu Asp Ile Phe Lys Thr Leu Thr Glu
305 310 315 320
Met Arg Lys Arg Ser Gly Val Gly Gly Phe Glu Phe Met Pro Ser Pro
325 330 335
Pro Pro Thr Tyr Tyr Lys Asn Leu Lys Asn Arg Ala Gly Asp Val Leu
340 345 350
Ser Asp Glu Gln Ile Leu Ala Cys Glu Glu Leu Gly Ile Leu Val Asp
355 360 365
Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro Val Gly
370 375 380
Asp Arg Pro Thr Ile Phe Ile Glu Ile Ile Gln Arg Ile Gly Cys Met
385 390 395 400
Leu Lys Asp Gly Lys Gly Gln Val Tyr Gln Lys Gly Gly Cys Gly Gly
405 410 415
Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Arg Ser Ile Glu Glu Tyr
420 425 430
Glu Lys Thr Leu Glu Ala Lys Gln Ala Asn Gln Val Ala Ala Ala
435 440 445
<210> 84
<211> 1344
<212> DNA
<213> NtHPPD-01核苷酸序列(Nicotiana tabacum)
<400> 84
atgggcaaat tagaaactgt caccaccacc tctgctgccg ctgccgatga ctcatcggag 60
ctgaccacca atttcaagct agtgggtttc aagaatttta ttcgaaccaa tccccgttcc 120
gattttttct ccgttaaaag cttccatcac atagaatttt ggtgcggcga tgctaccaat 180
acggctcgcc gtttctcttg gtctctcggg atgccaattg cagctaaatc cgacctctcc 240
actggaaact ccgttcacgc ttcgtacctc ctccgtcccg tttcaggttc gcttcagttc 300
ctcttcactg ctccttattc gccgtctatt tcgacgccgt catctgcagc catccccagt 360
ttctctactt ccacacaccg ctcttttacg acaactcacg ggctcggcgt ccgtgccgtt 420
gctttggaag tggagaatgc ttacacggcg ttctctgcga gtgtttcacg tggggcgaaa 480
cccatgtttg aacctgtgac tattgatgaa caagtcgcta tggctgaagt tcacttatac 540
ggcgacgtcg ttttgcggtt tgtgagctat ctgaaagatg cggacaacct cgttttcctc 600
ccaggtttcg aggccatgga tgaaacagca tcatacaaag aactagatta tgggattcgc 660
cggctggacc atgctgttgg gaatgtgccg gagctgggtc ctgtagtgga ttatatcaag 720
cgctttacgg ggtttcatga attcgccgag ttcacagctg aagatgttgg gactgctgag 780
agcgggctga actctatggt cctggcaaac aatgacgaaa cagtgctgct tccgttgaat 840
gagccggttt atggaaccaa aaggaagagt caaattcaaa cgtatttaga gcacaatgaa 900
ggggcaggag tgcaacattt agctttggtt acagaggata tatttaaaac attgacggaa 960
atgaggaaaa ggagtggagt tgggggtttt gaattcatgc cttcacctcc gcctacttat 1020
tataagaatt tgaagaatag ggcaggggat gtactgagtg atgaacagat tctggcttgc 1080
gaagaattgg gaatattagt ggatagggat gatcagggga ccctgctaca gatattcacc 1140
aagcctgtgg gggacaggcc aacaatattt atagaaataa ttcagagaat cggttgcatg 1200
ctcaaagacg gaaaaggtca ggtgtatcag aagggcggct gtggaggctt cggaaaggga 1260
aacttttcag agctctttag atccatcgaa gaatatgaga agacgcttga agccaaacaa 1320
gctaatcaag ttgctgctgc ttga 1344
<210> 85
<211> 1344
<212> DNA
<213> 人工序列-NtHPPD-02核苷酸序列(Artificial Sequence)
<400> 85
atggggaagc tggaaaccgt cactaccacc tcggcggcgg ctgctgatga ttcatcggaa 60
ttaacaacca acttcaagtt ggttggtttc aaaaatttca tcagaacaaa cccaaggtct 120
gatttcttct ccgtcaagag cttccaccac attgaattct ggtgcggtga cgcaactaac 180
acagcgcgcc gcttctcatg gagcctaggg atgccaattg cagctaagag tgatctttca 240
acaggaaaca gcgtgcacgc ctcctacctc ctccggcccg taagcggttc tttacagttc 300
ctgtttacag caccatattc accttctatt tcgactccta gttcagctgc aatcccatct 360
ttttccacat ctacacatag gtctttcact acaacacatg gactgggtgt tcgtgccgtc 420
gccctcgagg tggagaatgc ttatactgcc ttcagtgctt ctgtctccag aggcgccaag 480
ccaatgtttg aacctgtgac aattgatgaa caagtagcaa tggctgaggt tcatttgtac 540
ggcgatgttg tgctgcgatt tgtttcttat ctcaaggacg ccgacaacct ggtcttcctg 600
ccggggtttg aggccatgga tgaaactgct agttacaaag aacttgatta tggaatacgg 660
cgtttggatc atgctgtggg caatgttcca gaactcggcc cggtagttga ttacatcaag 720
aggtttactg gatttcatga atttgcagag tttaccgctg aagatgtggg aactgcggaa 780
agtgggctaa actccatggt tcttgcgaac aatgatgaga ctgtgctcct ccctctgaat 840
gagcctgttt atggcaccaa aagaaaatct cagatacaaa catatcttga gcataatgaa 900
ggagctgggg tgcagcacct tgctcttgtt actgaagaca tctttaagac tttgacagag 960
atgaggaaga gaagtggagt tggaggtttt gagttcatgc cttccccccc gccgacctac 1020
tacaagaatt tgaagaacag ggctggtgac gtcctctcag acgagcagat tttggcttgt 1080
gaggagcttg gcatcttggt agaccgggac gaccagggga cgcttctcca aattttcaca 1140
aaacccgtcg gggaccgccc caccatattt attgagatca tccagaggat aggctgcatg 1200
cttaaagatg gaaaaggtca agtgtaccag aagggtggtt gtggtgggtt tggcaaagga 1260
aatttttctg agctgttcag aagcatagaa gaatatgaga agacactgga agcaaaacaa 1320
gcaaatcaag ttgcggccgc gtga 1344
<210> 86
<211> 447
<212> PRT
<213> 人工序列-NtHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 86
Met Gly Lys Leu Glu Thr Val Thr Thr Thr Ser Ala Ala Ala Ala Asp
1 5 10 15
Asp Ser Ser Glu Leu Thr Thr Asn Phe Lys Leu Val Gly Phe Lys Asn
20 25 30
Phe Ile Arg Thr Asn Pro Arg Ser Asp Phe Phe Ser Val Lys Ser Phe
35 40 45
His His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Thr Ala Arg Arg
50 55 60
Phe Ser Trp Ser Leu Gly Met Pro Ile Ala Ala Lys Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Ser Val His Ala Ser Tyr Leu Leu Arg Pro Val Ser Gly
85 90 95
Ser Leu Gln Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Ile Ser Thr
100 105 110
Pro Ser Ser Ala Ala Ile Pro Ser Phe Ser Thr Ser Thr His Arg Ser
115 120 125
Phe Thr Thr Thr His Gly Leu Gly Val Arg Ala Val Ala Leu Glu Val
130 135 140
Glu Asn Ala Tyr Thr Ala Phe Ser Ala Ser Val Ser Arg Gly Ala Lys
145 150 155 160
Pro Met Phe Glu Pro Val Thr Ile Asp Glu Gln Val Ala Met Ala Glu
165 170 175
Val His Leu Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Leu Lys
180 185 190
Asp Ala Asp Asn Leu Val Phe Leu Pro Gly Phe Glu Ala Met Asp Glu
195 200 205
Thr Ala Ser Tyr Lys Glu Leu Asp Tyr Gly Ile Arg Arg Leu Asp His
210 215 220
Ala Val Gly Asn Val Pro Glu Leu Gly Pro Val Val Asp Tyr Ile Lys
225 230 235 240
Arg Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val
245 250 255
Gly Thr Ala Glu Ser Gly Leu Asn Ser Met Val Leu Ala Asn Asn Asp
260 265 270
Glu Thr Val Leu Leu Pro Leu Asn Glu Pro Val Tyr Gly Thr Lys Arg
275 280 285
Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala Gly Val
290 295 300
Gln His Leu Ala Leu Val Thr Glu Asp Ile Phe Lys Thr Leu Thr Glu
305 310 315 320
Met Arg Lys Arg Ser Gly Val Gly Gly Phe Glu Phe Met Pro Ser Pro
325 330 335
Pro Pro Thr Tyr Tyr Lys Asn Leu Lys Asn Arg Ala Gly Asp Val Leu
340 345 350
Ser Asp Glu Gln Ile Leu Ala Cys Glu Glu Leu Gly Ile Leu Val Asp
355 360 365
Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro Val Gly
370 375 380
Asp Arg Pro Thr Ile Phe Ile Glu Ile Ile Gln Arg Ile Gly Cys Met
385 390 395 400
Leu Lys Asp Gly Lys Gly Gln Val Tyr Gln Lys Gly Gly Cys Gly Gly
405 410 415
Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Arg Ser Ile Glu Glu Tyr
420 425 430
Glu Lys Thr Leu Glu Ala Lys Gln Ala Asn Gln Val Ala Ala Ala
435 440 445
<210> 87
<211> 1344
<212> DNA
<213> 人工序列-NtHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 87
atgggcaaat tagaaactgt caccaccacc tctgctgccg ctgccgatga ctcatcggag 60
ctgaccacca atttcaagct agtgggtttc aagaatttta ttcgaaccaa tccccgttcc 120
gattttttct ccgttaaaag cttccatcac atagaatttt ggtgcggcga tgctaccaat 180
acggctcgcc gtttctcttg gtctctcggg atgccaattg cagctaaatc cgacctctcc 240
actggaaact ccgttcacgc ttcgtacctc ctccgtcccg tttcaggttc gcttcagttc 300
ctcttcactg ctccttattc gccgtctatt tcgacgccgt catctgcagc catccccagt 360
ttctctactt ccacacaccg ctcttttacg acaactcacg ggctcggcgt ccgtgccgtt 420
gctttggaag tggagaatgc ttacacggcg ttctctgcga gtgtttcacg tggggcgaaa 480
cccatgtttg aacctgtgac tattgatgaa caagtcgcta tggctgaagt tcacttatac 540
ggcgacgtcg ttttgcggtt tgtgagctat ctgaaagatg cggacaacct cgttttcctc 600
ccaggtttcg aggccatgga tgaaacagca tcatacaaag aactagatta tgggattcgc 660
cggctggacc atgctgttgg gaatgtgccg gagctgggtc ctgtagtgga ttatatcaag 720
cgctttacgg ggtttcatga attcgccgag ttcacagctg aagatgttgg gactgctgag 780
agcgggctga actctatggt cctggcaaac aatgacgaaa cagtgctgct tccgttgaat 840
gagccggttt atggaaccaa aaggaagagt caaattcaaa cgtatttaga gcacaatgaa 900
ggggcaggag tgcaacattt agctttggtt acagaggata tatttaaaac attgacggaa 960
atgaggaaaa ggagtggagt tgggggtttt gaattcatgc cttcacctcc gcctacttat 1020
tataagaatt tgaagaatag ggcaggggat gtactgagtg atgaacagat tctggcttgc 1080
gaagaattgg gaatattagt ggatagggat gatcagggga ccctgctaca gatagctacc 1140
aagcctgtgg gggacaggcc aacaatattt atagaaataa ttcagagaat cggttgcatg 1200
ctcaaagacg gaaaaggtca ggtgtatcag aagggcggct gtggaggctt cggaaaggga 1260
aacttttcag agctctttag atccatcgaa gaatatgaga agacgcttga agccaaacaa 1320
gctaatcaag ttgctgctgc ttga 1344
<210> 88
<211> 1344
<212> DNA
<213> 人工序列-NtHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 88
atggggaagc tggaaaccgt cactaccacc tcggcggcgg ctgctgatga ttcatcggaa 60
ttaacaacca acttcaagtt ggttggtttc aaaaatttca tcagaacaaa cccaaggtct 120
gatttcttct ccgtcaagag cttccaccac attgaattct ggtgcggtga cgcaactaac 180
acagcgcgcc gcttctcatg gagcctaggg atgccaattg cagctaagag tgatctttca 240
acaggaaaca gcgtgcacgc ctcctacctc ctccggcccg taagcggttc tttacagttc 300
ctgtttacag caccatattc accttctatt tcgactccta gttcagctgc aatcccatct 360
ttttccacat ctacacatag gtctttcact acaacacatg gactgggtgt tcgtgccgtc 420
gccctcgagg tggagaatgc ttatactgcc ttcagtgctt ctgtctccag aggcgccaag 480
ccaatgtttg aacctgtgac aattgatgaa caagtagcaa tggctgaggt tcatttgtac 540
ggcgatgttg tgctgcgatt tgtttcttat ctcaaggacg ccgacaacct ggtcttcctg 600
ccggggtttg aggccatgga tgaaactgct agttacaaag aacttgatta tggaatacgg 660
cgtttggatc atgctgtggg caatgttcca gaactcggcc cggtagttga ttacatcaag 720
aggtttactg gatttcatga atttgcagag tttaccgctg aagatgtggg aactgcggaa 780
agtgggctaa actccatggt tcttgcgaac aatgatgaga ctgtgctcct ccctctgaat 840
gagcctgttt atggcaccaa aagaaaatct cagatacaaa catatcttga gcataatgaa 900
ggagctgggg tgcagcacct tgctcttgtt actgaagaca tctttaagac tttgacagag 960
atgaggaaga gaagtggagt tggaggtttt gagttcatgc cttccccccc gccgacctac 1020
tacaagaatt tgaagaacag ggctggtgac gtcctctcag acgagcagat tttggcttgt 1080
gaggagcttg gcatcttggt agaccgggac gaccagggga cgcttctcca aattgccaca 1140
aaacccgtcg gggaccgccc caccatattt attgagatca tccagaggat aggctgcatg 1200
cttaaagatg gaaaaggtca agtgtaccag aagggtggtt gtggtgggtt tggcaaagga 1260
aatttttctg agctgttcag aagcatagaa gaatatgaga agacactgga agcaaaacaa 1320
gcaaatcaag ttgcggccgc gtga 1344
<210> 89
<211> 447
<212> PRT
<213> 人工序列-NtHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 89
Met Gly Lys Leu Glu Thr Val Thr Thr Thr Ser Ala Ala Ala Ala Asp
1 5 10 15
Asp Ser Ser Glu Leu Thr Thr Asn Phe Lys Leu Val Gly Phe Lys Asn
20 25 30
Phe Ile Arg Thr Asn Pro Arg Ser Asp Phe Phe Ser Val Lys Ser Phe
35 40 45
His His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Thr Ala Arg Arg
50 55 60
Phe Ser Trp Ser Leu Gly Met Pro Ile Ala Ala Lys Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Ser Val His Ala Ser Tyr Leu Leu Arg Pro Val Ser Gly
85 90 95
Ser Leu Gln Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Ile Ser Thr
100 105 110
Pro Ser Ser Ala Ala Ile Pro Ser Phe Ser Thr Ser Thr His Arg Ser
115 120 125
Phe Thr Thr Thr His Gly Leu Gly Val Arg Ala Val Ala Leu Glu Val
130 135 140
Glu Asn Ala Tyr Thr Ala Phe Ser Ala Ser Val Ser Arg Gly Ala Lys
145 150 155 160
Pro Met Phe Glu Pro Val Thr Ile Asp Glu Gln Val Ala Met Ala Glu
165 170 175
Val His Leu Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Leu Lys
180 185 190
Asp Ala Asp Asn Leu Val Phe Leu Pro Gly Phe Glu Ala Met Asp Glu
195 200 205
Thr Ala Ser Tyr Lys Glu Leu Asp Tyr Gly Ile Arg Arg Leu Asp His
210 215 220
Ala Val Gly Asn Val Pro Glu Leu Gly Pro Val Val Asp Tyr Ile Lys
225 230 235 240
Arg Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val
245 250 255
Gly Thr Ala Glu Ser Gly Leu Asn Ser Met Val Leu Ala Asn Asn Asp
260 265 270
Glu Thr Val Leu Leu Pro Leu Asn Glu Pro Val Tyr Gly Thr Lys Arg
275 280 285
Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala Gly Val
290 295 300
Gln His Leu Ala Leu Val Thr Glu Asp Ile Phe Lys Thr Leu Thr Glu
305 310 315 320
Met Arg Lys Arg Ser Gly Val Gly Gly Phe Glu Phe Met Pro Ser Pro
325 330 335
Pro Pro Thr Tyr Tyr Lys Asn Leu Lys Asn Arg Ala Gly Asp Val Leu
340 345 350
Ser Asp Glu Gln Ile Leu Ala Cys Glu Glu Leu Gly Ile Leu Val Asp
355 360 365
Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro Val Gly
370 375 380
Asp Arg Pro Thr Ile Trp Ile Glu Ile Ile Gln Arg Ile Gly Cys Met
385 390 395 400
Leu Lys Asp Gly Lys Gly Gln Val Tyr Gln Lys Gly Gly Cys Gly Gly
405 410 415
Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Arg Ser Ile Glu Glu Tyr
420 425 430
Glu Lys Thr Leu Glu Ala Lys Gln Ala Asn Gln Val Ala Ala Ala
435 440 445
<210> 90
<211> 1344
<212> DNA
<213> 人工序列-NtHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 90
atgggcaaat tagaaactgt caccaccacc tctgctgccg ctgccgatga ctcatcggag 60
ctgaccacca atttcaagct agtgggtttc aagaatttta ttcgaaccaa tccccgttcc 120
gattttttct ccgttaaaag cttccatcac atagaatttt ggtgcggcga tgctaccaat 180
acggctcgcc gtttctcttg gtctctcggg atgccaattg cagctaaatc cgacctctcc 240
actggaaact ccgttcacgc ttcgtacctc ctccgtcccg tttcaggttc gcttcagttc 300
ctcttcactg ctccttattc gccgtctatt tcgacgccgt catctgcagc catccccagt 360
ttctctactt ccacacaccg ctcttttacg acaactcacg ggctcggcgt ccgtgccgtt 420
gctttggaag tggagaatgc ttacacggcg ttctctgcga gtgtttcacg tggggcgaaa 480
cccatgtttg aacctgtgac tattgatgaa caagtcgcta tggctgaagt tcacttatac 540
ggcgacgtcg ttttgcggtt tgtgagctat ctgaaagatg cggacaacct cgttttcctc 600
ccaggtttcg aggccatgga tgaaacagca tcatacaaag aactagatta tgggattcgc 660
cggctggacc atgctgttgg gaatgtgccg gagctgggtc ctgtagtgga ttatatcaag 720
cgctttacgg ggtttcatga attcgccgag ttcacagctg aagatgttgg gactgctgag 780
agcgggctga actctatggt cctggcaaac aatgacgaaa cagtgctgct tccgttgaat 840
gagccggttt atggaaccaa aaggaagagt caaattcaaa cgtatttaga gcacaatgaa 900
ggggcaggag tgcaacattt agctttggtt acagaggata tatttaaaac attgacggaa 960
atgaggaaaa ggagtggagt tgggggtttt gaattcatgc cttcacctcc gcctacttat 1020
tataagaatt tgaagaatag ggcaggggat gtactgagtg atgaacagat tctggcttgc 1080
gaagaattgg gaatattagt ggatagggat gatcagggga ccctgctaca gatattcacc 1140
aagcctgtgg gggacaggcc aacaatatgg atagaaataa ttcagagaat cggttgcatg 1200
ctcaaagacg gaaaaggtca ggtgtatcag aagggcggct gtggaggctt cggaaaggga 1260
aacttttcag agctctttag atccatcgaa gaatatgaga agacgcttga agccaaacaa 1320
gctaatcaag ttgctgctgc ttga 1344
<210> 91
<211> 1344
<212> DNA
<213> 人工序列-NtHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 91
atggggaagc tggaaaccgt cactaccacc tcggcggcgg ctgctgatga ttcatcggaa 60
ttaacaacca acttcaagtt ggttggtttc aaaaatttca tcagaacaaa cccaaggtct 120
gatttcttct ccgtcaagag cttccaccac attgaattct ggtgcggtga cgcaactaac 180
acagcgcgcc gcttctcatg gagcctaggg atgccaattg cagctaagag tgatctttca 240
acaggaaaca gcgtgcacgc ctcctacctc ctccggcccg taagcggttc tttacagttc 300
ctgtttacag caccatattc accttctatt tcgactccta gttcagctgc aatcccatct 360
ttttccacat ctacacatag gtctttcact acaacacatg gactgggtgt tcgtgccgtc 420
gccctcgagg tggagaatgc ttatactgcc ttcagtgctt ctgtctccag aggcgccaag 480
ccaatgtttg aacctgtgac aattgatgaa caagtagcaa tggctgaggt tcatttgtac 540
ggcgatgttg tgctgcgatt tgtttcttat ctcaaggacg ccgacaacct ggtcttcctg 600
ccggggtttg aggccatgga tgaaactgct agttacaaag aacttgatta tggaatacgg 660
cgtttggatc atgctgtggg caatgttcca gaactcggcc cggtagttga ttacatcaag 720
aggtttactg gatttcatga atttgcagag tttaccgctg aagatgtggg aactgcggaa 780
agtgggctaa actccatggt tcttgcgaac aatgatgaga ctgtgctcct ccctctgaat 840
gagcctgttt atggcaccaa aagaaaatct cagatacaaa catatcttga gcataatgaa 900
ggagctgggg tgcagcacct tgctcttgtt actgaagaca tctttaagac tttgacagag 960
atgaggaaga gaagtggagt tggaggtttt gagttcatgc cttccccccc gccgacctac 1020
tacaagaatt tgaagaacag ggctggtgac gtcctctcag acgagcagat tttggcttgt 1080
gaggagcttg gcatcttggt agaccgggac gaccagggga cgcttctcca aattttcaca 1140
aaacccgtcg gggaccgccc caccatatgg attgagatca tccagaggat aggctgcatg 1200
cttaaagatg gaaaaggtca agtgtaccag aagggtggtt gtggtgggtt tggcaaagga 1260
aatttttctg agctgttcag aagcatagaa gaatatgaga agacactgga agcaaaacaa 1320
gcaaatcaag ttgcggccgc gtga 1344
<210> 92
<211> 447
<212> PRT
<213> 人工序列-NtHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 92
Met Gly Lys Leu Glu Thr Val Thr Thr Thr Ser Ala Ala Ala Ala Asp
1 5 10 15
Asp Ser Ser Glu Leu Thr Thr Asn Phe Lys Leu Val Gly Phe Lys Asn
20 25 30
Phe Ile Arg Thr Asn Pro Arg Ser Asp Phe Phe Ser Val Lys Ser Phe
35 40 45
His His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Thr Ala Arg Arg
50 55 60
Phe Ser Trp Ser Leu Gly Met Pro Ile Ala Ala Lys Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Ser Val His Ala Ser Tyr Leu Leu Arg Pro Val Ser Gly
85 90 95
Ser Leu Gln Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Ile Ser Thr
100 105 110
Pro Ser Ser Ala Ala Ile Pro Ser Phe Ser Thr Ser Thr His Arg Ser
115 120 125
Phe Thr Thr Thr His Gly Leu Gly Val Arg Ala Val Ala Leu Glu Val
130 135 140
Glu Asn Ala Tyr Thr Ala Phe Ser Ala Ser Val Ser Arg Gly Ala Lys
145 150 155 160
Pro Met Phe Glu Pro Val Thr Ile Asp Glu Gln Val Ala Met Ala Glu
165 170 175
Val His Leu Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Leu Lys
180 185 190
Asp Ala Asp Asn Leu Val Phe Leu Pro Gly Phe Glu Ala Met Asp Glu
195 200 205
Thr Ala Ser Tyr Lys Glu Leu Asp Tyr Gly Ile Arg Arg Leu Asp His
210 215 220
Ala Val Gly Asn Val Pro Glu Leu Gly Pro Val Val Asp Tyr Ile Lys
225 230 235 240
Arg Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val
245 250 255
Gly Thr Ala Glu Ser Gly Leu Asn Ser Met Val Leu Ala Asn Asn Asp
260 265 270
Glu Thr Val Leu Leu Pro Leu Asn Glu Pro Val Tyr Gly Thr Lys Arg
275 280 285
Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala Gly Val
290 295 300
Gln His Leu Ala Leu Val Thr Glu Asp Ile Phe Lys Thr Leu Thr Glu
305 310 315 320
Met Arg Lys Arg Ser Gly Val Gly Gly Phe Glu Phe Met Pro Ser Pro
325 330 335
Pro Pro Thr Tyr Tyr Lys Asn Leu Lys Asn Arg Ala Gly Asp Val Leu
340 345 350
Ser Asp Glu Gln Ile Leu Ala Cys Glu Glu Leu Gly Ile Leu Val Asp
355 360 365
Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro Val Gly
370 375 380
Asp Arg Pro Thr Ile Trp Ile Glu Ile Ile Gln Arg Ile Gly Cys Met
385 390 395 400
Leu Lys Asp Gly Lys Gly Gln Val Tyr Gln Lys Gly Gly Cys Gly Gly
405 410 415
Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Arg Ser Ile Glu Glu Tyr
420 425 430
Glu Lys Thr Leu Glu Ala Lys Gln Ala Asn Gln Val Ala Ala Ala
435 440 445
<210> 93
<211> 1344
<212> DNA
<213> 人工序列-NtHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 93
atgggcaaat tagaaactgt caccaccacc tctgctgccg ctgccgatga ctcatcggag 60
ctgaccacca atttcaagct agtgggtttc aagaatttta ttcgaaccaa tccccgttcc 120
gattttttct ccgttaaaag cttccatcac atagaatttt ggtgcggcga tgctaccaat 180
acggctcgcc gtttctcttg gtctctcggg atgccaattg cagctaaatc cgacctctcc 240
actggaaact ccgttcacgc ttcgtacctc ctccgtcccg tttcaggttc gcttcagttc 300
ctcttcactg ctccttattc gccgtctatt tcgacgccgt catctgcagc catccccagt 360
ttctctactt ccacacaccg ctcttttacg acaactcacg ggctcggcgt ccgtgccgtt 420
gctttggaag tggagaatgc ttacacggcg ttctctgcga gtgtttcacg tggggcgaaa 480
cccatgtttg aacctgtgac tattgatgaa caagtcgcta tggctgaagt tcacttatac 540
ggcgacgtcg ttttgcggtt tgtgagctat ctgaaagatg cggacaacct cgttttcctc 600
ccaggtttcg aggccatgga tgaaacagca tcatacaaag aactagatta tgggattcgc 660
cggctggacc atgctgttgg gaatgtgccg gagctgggtc ctgtagtgga ttatatcaag 720
cgctttacgg ggtttcatga attcgccgag ttcacagctg aagatgttgg gactgctgag 780
agcgggctga actctatggt cctggcaaac aatgacgaaa cagtgctgct tccgttgaat 840
gagccggttt atggaaccaa aaggaagagt caaattcaaa cgtatttaga gcacaatgaa 900
ggggcaggag tgcaacattt agctttggtt acagaggata tatttaaaac attgacggaa 960
atgaggaaaa ggagtggagt tgggggtttt gaattcatgc cttcacctcc gcctacttat 1020
tataagaatt tgaagaatag ggcaggggat gtactgagtg atgaacagat tctggcttgc 1080
gaagaattgg gaatattagt ggatagggat gatcagggga ccctgctaca gatagctacc 1140
aagcctgtgg gggacaggcc aacaatatgg atagaaataa ttcagagaat cggttgcatg 1200
ctcaaagacg gaaaaggtca ggtgtatcag aagggcggct gtggaggctt cggaaaggga 1260
aacttttcag agctctttag atccatcgaa gaatatgaga agacgcttga agccaaacaa 1320
gctaatcaag ttgctgctgc ttga 1344
<210> 94
<211> 1344
<212> DNA
<213> 人工序列-NtHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 94
atggggaagc tggaaaccgt cactaccacc tcggcggcgg ctgctgatga ttcatcggaa 60
ttaacaacca acttcaagtt ggttggtttc aaaaatttca tcagaacaaa cccaaggtct 120
gatttcttct ccgtcaagag cttccaccac attgaattct ggtgcggtga cgcaactaac 180
acagcgcgcc gcttctcatg gagcctaggg atgccaattg cagctaagag tgatctttca 240
acaggaaaca gcgtgcacgc ctcctacctc ctccggcccg taagcggttc tttacagttc 300
ctgtttacag caccatattc accttctatt tcgactccta gttcagctgc aatcccatct 360
ttttccacat ctacacatag gtctttcact acaacacatg gactgggtgt tcgtgccgtc 420
gccctcgagg tggagaatgc ttatactgcc ttcagtgctt ctgtctccag aggcgccaag 480
ccaatgtttg aacctgtgac aattgatgaa caagtagcaa tggctgaggt tcatttgtac 540
ggcgatgttg tgctgcgatt tgtttcttat ctcaaggacg ccgacaacct ggtcttcctg 600
ccggggtttg aggccatgga tgaaactgct agttacaaag aacttgatta tggaatacgg 660
cgtttggatc atgctgtggg caatgttcca gaactcggcc cggtagttga ttacatcaag 720
aggtttactg gatttcatga atttgcagag tttaccgctg aagatgtggg aactgcggaa 780
agtgggctaa actccatggt tcttgcgaac aatgatgaga ctgtgctcct ccctctgaat 840
gagcctgttt atggcaccaa aagaaaatct cagatacaaa catatcttga gcataatgaa 900
ggagctgggg tgcagcacct tgctcttgtt actgaagaca tctttaagac tttgacagag 960
atgaggaaga gaagtggagt tggaggtttt gagttcatgc cttccccccc gccgacctac 1020
tacaagaatt tgaagaacag ggctggtgac gtcctctcag acgagcagat tttggcttgt 1080
gaggagcttg gcatcttggt agaccgggac gaccagggga cgcttctcca aattgccaca 1140
aaacccgtcg gggaccgccc caccatatgg attgagatca tccagaggat aggctgcatg 1200
cttaaagatg gaaaaggtca agtgtaccag aagggtggtt gtggtgggtt tggcaaagga 1260
aatttttctg agctgttcag aagcatagaa gaatatgaga agacactgga agcaaaacaa 1320
gcaaatcaag ttgcggccgc gtga 1344
<210> 95
<211> 446
<212> PRT
<213> 水稻野生型HPPD(OsHPPD的氨基酸序列Oryza sativa)
<400> 95
Met Pro Pro Thr Pro Thr Pro Thr Ala Thr Thr Gly Ala Val Ser Ala
1 5 10 15
Ala Ala Ala Ala Gly Glu Asn Ala Gly Phe Arg Leu Val Gly His Arg
20 25 30
Arg Phe Val Arg Ala Asn Pro Arg Ser Asp Arg Phe Gln Ala Leu Ala
35 40 45
Phe His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly
50 55 60
Arg Phe Ala Phe Ala Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu
65 70 75 80
Ser Thr Gly Asn Ser Ala His Ala Ser Leu Leu Leu Arg Ser Ala Ser
85 90 95
Val Ala Phe Leu Phe Thr Ala Pro Tyr Gly Gly Asp His Gly Val Gly
100 105 110
Ala Asp Ala Ala Thr Thr Ala Ser Ile Pro Ser Phe Ser Pro Gly Ala
115 120 125
Ala Arg Arg Phe Ala Ala Asp His Gly Leu Ala Val His Ala Val Ala
130 135 140
Leu Arg Val Ala Asp Ala Ala Asp Ala Phe Arg Ala Ser Val Ala Ala
145 150 155 160
Gly Ala Arg Pro Ala Phe Gln Pro Ala Asp Leu Gly Gly Gly Phe Gly
165 170 175
Leu Ala Glu Val Glu Leu Tyr Gly Asp Val Val Leu Arg Phe Val Ser
180 185 190
His Pro Asp Gly Ala Asp Ala Pro Phe Leu Pro Gly Phe Glu Gly Val
195 200 205
Ser Asn Pro Gly Ala Val Asp Tyr Gly Leu Arg Arg Phe Asp His Val
210 215 220
Val Gly Asn Val Pro Glu Leu Ala Pro Val Ala Ala Tyr Ile Ser Gly
225 230 235 240
Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val Gly
245 250 255
Thr Ala Glu Ser Gly Leu Asn Ser Val Val Leu Ala Asn Asn Ala Glu
260 265 270
Thr Val Leu Leu Pro Leu Asn Glu Pro Val His Gly Thr Lys Arg Arg
275 280 285
Ser Gln Ile Gln Thr Tyr Leu Asp His His Gly Gly Pro Gly Val Gln
290 295 300
His Ile Ala Leu Ala Ser Asp Asp Val Leu Gly Thr Leu Arg Glu Met
305 310 315 320
Arg Ala Arg Ser Ala Met Gly Gly Phe Glu Phe Leu Ala Pro Pro Pro
325 330 335
Pro Asn Tyr Tyr Asp Gly Val Arg Arg Arg Ala Gly Asp Val Leu Ser
340 345 350
Glu Glu Gln Ile Asn Glu Cys Gln Glu Leu Gly Val Leu Val Asp Arg
355 360 365
Asp Asp Gln Gly Val Leu Leu Gln Ile Phe Thr Lys Pro Val Gly Asp
370 375 380
Arg Pro Thr Phe Phe Leu Glu Met Ile Gln Arg Ile Gly Cys Met Glu
385 390 395 400
Lys Asp Glu Ser Gly Gln Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe
405 410 415
Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu
420 425 430
Lys Ser Leu Glu Ala Lys Gln Ala Pro Thr Val Gln Gly Ser
435 440 445
<210> 96
<211> 1341
<212> DNA
<213> OsHPPD-01核苷酸序列(Oryza sativa)
<400> 96
atgcctccca ctcccacccc caccgccacc accggcgccg tctcggccgc tgcggcggcg 60
ggggagaacg cggggttccg cctcgtcggg caccgccgct tcgtccgcgc caacccgcgg 120
agcgaccggt tccaggcgct cgcgttccac cacgtcgagc tctggtgcgc cgacgccgcg 180
tccgccgcgg gccggttcgc cttcgccctg ggcgcgccgc tcgccgccag gtccgacctc 240
tccacgggga actccgcgca cgcctccctc ctcctccgct ccgcctccgt cgcgttcctc 300
ttcaccgccc cctacggcgg cgaccacggc gtcggcgcgg acgcggccac caccgcctcc 360
atcccttcct tctccccagg cgccgcgcgg aggttcgccg cggaccacgg cctcgcggtg 420
cacgccgtgg cgctgcgcgt cgccgacgcg gccgacgcct tccgcgccag cgtcgcggcc 480
ggtgcgcgcc cggcgttcca gcccgccgac ctcggcggtg gcttcggcct cgcggaggtg 540
gagctctacg gcgacgtcgt gctccgcttc gtcagccacc cggacggcgc cgacgcgccc 600
ttcctcccgg gtttcgaggg cgtcagcaac ccgggcgccg tggactacgg cctccgccgg 660
ttcgaccacg tcgtcggcaa cgtgccggag ctcgctccgg tagccgcgta catctccggg 720
ttcaccgggt tccacgagtt cgccgagttc accgccgagg acgtgggcac cgccgagagc 780
ggcctcaact cggtggtgct cgccaacaac gcggagaccg tgctgctgcc gctcaacgag 840
ccggtgcacg gcaccaagcg gcggagccag atacagacgt acctggacca ccacggcggc 900
ccgggggtgc agcacatcgc gctggccagc gacgacgtgc tcgggacgct gagggagatg 960
cgggcgcgct ccgccatggg cggcttcgag ttcttggcgc cgccgccgcc caactactac 1020
gacggcgtgc ggcggcgcgc cggggacgtg ctctcggagg agcagatcaa cgagtgccag 1080
gagctcgggg tgctcgtgga cagggatgac cagggggtgt tgctccagat cttcaccaag 1140
ccagtaggag acaggccaac ctttttcttg gagatgatac aaaggattgg gtgcatggag 1200
aaggatgaga gtgggcagga gtaccagaag ggcggctgcg gcgggtttgg gaagggcaac 1260
ttctcggagc tgttcaagtc cattgaggag tatgagaaat cccttgaagc caagcaagcc 1320
cctacagttc aaggatccta g 1341
<210> 97
<211> 1341
<212> DNA
<213> 人工序列-OsHPPD-02核苷酸序列(Artificial Sequence)
<400> 97
atgccaccca cgccaacacc aacggccacc actggtgccg tctcggccgc ggcagcggcc 60
ggtgagaatg ctggtttcag gctagtcggc caccgccgct tcgtgagggc aaatccaaga 120
tctgatcggt tccaagcact tgctttccac catgttgagc tgtggtgcgc tgacgccgct 180
tcagctgccg ggaggtttgc ttttgcttta ggggcgccgc tcgccgcaag gagtgatctt 240
tccaccggaa attctgctca tgcttctctt ctacttagat cagcaagtgt tgcatttctg 300
ttcactgcac cttatggcgg agaccatggt gtcggggccg atgctgcaac aacagctagc 360
ataccatcat tttctcccgg cgccgcaaga agatttgcag ccgatcatgg cctggcagtt 420
catgctgtgg cattgagggt cgccgacgcg gctgatgcgt tccgcgccag cgtggctgct 480
ggagctaggc cggcgttcca gcctgctgac cttgggggtg gatttggatt ggcggaggtt 540
gaattatatg gtgatgttgt gctccgtttt gtttcccatc cagatggtgc tgatgctcca 600
tttctacctg gatttgaagg ggtgtcaaac cccggcgcgg tggattatgg actgcgtcga 660
tttgatcacg tggtaggtaa tgttcctgag ttggctcccg tggcggccta catttcaggc 720
tttactggct tccatgaatt tgccgagttc accgcggaag atgtgggaac tgctgaaagt 780
ggattgaact ctgttgtcct ggcgaacaat gcagagactg tattgctccc cttgaatgag 840
cctgtgcacg gcaccaaacg gcgatctcaa attcagacat acctcgacca ccacgggggc 900
cctggtgttc agcatattgc tctagccagc gacgacgtac tgggaacact gagggagatg 960
agagcccgtt ccgccatggg tggctttgag tttctggccc ctccgccgcc aaactactac 1020
gacggcgtca gacggcgggc gggtgacgtc ttgagtgagg agcagatcaa tgaatgtcaa 1080
gaacttgggg ttcttgttga tagagatgat caaggagtgc tcctccaaat attcacaaaa 1140
cctgttggcg accgcccgac cttcttcctt gaaatgatcc aaaggatcgg ctgcatggag 1200
aaggatgaat cgggccagga gtaccagaag ggtgggtgtg gtggctttgg gaagggcaac 1260
ttcagcgagc tgttcaagtc aattgaagaa tatgaaaaat cattggaggc aaagcaagct 1320
ccgacggtgc agggaagctg a 1341
<210> 98
<211> 446
<212> PRT
<213> 人工序列-OsHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 98
Met Pro Pro Thr Pro Thr Pro Thr Ala Thr Thr Gly Ala Val Ser Ala
1 5 10 15
Ala Ala Ala Ala Gly Glu Asn Ala Gly Phe Arg Leu Val Gly His Arg
20 25 30
Arg Phe Val Arg Ala Asn Pro Arg Ser Asp Arg Phe Gln Ala Leu Ala
35 40 45
Phe His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly
50 55 60
Arg Phe Ala Phe Ala Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu
65 70 75 80
Ser Thr Gly Asn Ser Ala His Ala Ser Leu Leu Leu Arg Ser Ala Ser
85 90 95
Val Ala Phe Leu Phe Thr Ala Pro Tyr Gly Gly Asp His Gly Val Gly
100 105 110
Ala Asp Ala Ala Thr Thr Ala Ser Ile Pro Ser Phe Ser Pro Gly Ala
115 120 125
Ala Arg Arg Phe Ala Ala Asp His Gly Leu Ala Val His Ala Val Ala
130 135 140
Leu Arg Val Ala Asp Ala Ala Asp Ala Phe Arg Ala Ser Val Ala Ala
145 150 155 160
Gly Ala Arg Pro Ala Phe Gln Pro Ala Asp Leu Gly Gly Gly Phe Gly
165 170 175
Leu Ala Glu Val Glu Leu Tyr Gly Asp Val Val Leu Arg Phe Val Ser
180 185 190
His Pro Asp Gly Ala Asp Ala Pro Phe Leu Pro Gly Phe Glu Gly Val
195 200 205
Ser Asn Pro Gly Ala Val Asp Tyr Gly Leu Arg Arg Phe Asp His Val
210 215 220
Val Gly Asn Val Pro Glu Leu Ala Pro Val Ala Ala Tyr Ile Ser Gly
225 230 235 240
Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val Gly
245 250 255
Thr Ala Glu Ser Gly Leu Asn Ser Val Val Leu Ala Asn Asn Ala Glu
260 265 270
Thr Val Leu Leu Pro Leu Asn Glu Pro Val His Gly Thr Lys Arg Arg
275 280 285
Ser Gln Ile Gln Thr Tyr Leu Asp His His Gly Gly Pro Gly Val Gln
290 295 300
His Ile Ala Leu Ala Ser Asp Asp Val Leu Gly Thr Leu Arg Glu Met
305 310 315 320
Arg Ala Arg Ser Ala Met Gly Gly Phe Glu Phe Leu Ala Pro Pro Pro
325 330 335
Pro Asn Tyr Tyr Asp Gly Val Arg Arg Arg Ala Gly Asp Val Leu Ser
340 345 350
Glu Glu Gln Ile Asn Glu Cys Gln Glu Leu Gly Val Leu Val Asp Arg
355 360 365
Asp Asp Gln Gly Val Leu Leu Gln Ile Ala Thr Lys Pro Val Gly Asp
370 375 380
Arg Pro Thr Phe Phe Leu Glu Met Ile Gln Arg Ile Gly Cys Met Glu
385 390 395 400
Lys Asp Glu Ser Gly Gln Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe
405 410 415
Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu
420 425 430
Lys Ser Leu Glu Ala Lys Gln Ala Pro Thr Val Gln Gly Ser
435 440 445
<210> 99
<211> 1341
<212> DNA
<213> 人工序列-OsHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 99
atgcctccca ctcccacccc caccgccacc accggcgccg tctcggccgc tgcggcggcg 60
ggggagaacg cggggttccg cctcgtcggg caccgccgct tcgtccgcgc caacccgcgg 120
agcgaccggt tccaggcgct cgcgttccac cacgtcgagc tctggtgcgc cgacgccgcg 180
tccgccgcgg gccggttcgc cttcgccctg ggcgcgccgc tcgccgccag gtccgacctc 240
tccacgggga actccgcgca cgcctccctc ctcctccgct ccgcctccgt cgcgttcctc 300
ttcaccgccc cctacggcgg cgaccacggc gtcggcgcgg acgcggccac caccgcctcc 360
atcccttcct tctccccagg cgccgcgcgg aggttcgccg cggaccacgg cctcgcggtg 420
cacgccgtgg cgctgcgcgt cgccgacgcg gccgacgcct tccgcgccag cgtcgcggcc 480
ggtgcgcgcc cggcgttcca gcccgccgac ctcggcggtg gcttcggcct cgcggaggtg 540
gagctctacg gcgacgtcgt gctccgcttc gtcagccacc cggacggcgc cgacgcgccc 600
ttcctcccgg gtttcgaggg cgtcagcaac ccgggcgccg tggactacgg cctccgccgg 660
ttcgaccacg tcgtcggcaa cgtgccggag ctcgctccgg tagccgcgta catctccggg 720
ttcaccgggt tccacgagtt cgccgagttc accgccgagg acgtgggcac cgccgagagc 780
ggcctcaact cggtggtgct cgccaacaac gcggagaccg tgctgctgcc gctcaacgag 840
ccggtgcacg gcaccaagcg gcggagccag atacagacgt acctggacca ccacggcggc 900
ccgggggtgc agcacatcgc gctggccagc gacgacgtgc tcgggacgct gagggagatg 960
cgggcgcgct ccgccatggg cggcttcgag ttcttggcgc cgccgccgcc caactactac 1020
gacggcgtgc ggcggcgcgc cggggacgtg ctctcggagg agcagatcaa cgagtgccag 1080
gagctcgggg tgctcgtgga cagggatgac cagggggtgt tgctccagat cgctaccaag 1140
ccagtaggag acaggccaac ctttttcttg gagatgatac aaaggattgg gtgcatggag 1200
aaggatgaga gtgggcagga gtaccagaag ggcggctgcg gcgggtttgg gaagggcaac 1260
ttctcggagc tgttcaagtc cattgaggag tatgagaaat cccttgaagc caagcaagcc 1320
cctacagttc aaggatccta g 1341
<210> 100
<211> 1341
<212> DNA
<213> 人工序列-OsHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 100
atgccaccca cgccaacacc aacggccacc actggtgccg tctcggccgc ggcagcggcc 60
ggtgagaatg ctggtttcag gctagtcggc caccgccgct tcgtgagggc aaatccaaga 120
tctgatcggt tccaagcact tgctttccac catgttgagc tgtggtgcgc tgacgccgct 180
tcagctgccg ggaggtttgc ttttgcttta ggggcgccgc tcgccgcaag gagtgatctt 240
tccaccggaa attctgctca tgcttctctt ctacttagat cagcaagtgt tgcatttctg 300
ttcactgcac cttatggcgg agaccatggt gtcggggccg atgctgcaac aacagctagc 360
ataccatcat tttctcccgg cgccgcaaga agatttgcag ccgatcatgg cctggcagtt 420
catgctgtgg cattgagggt cgccgacgcg gctgatgcgt tccgcgccag cgtggctgct 480
ggagctaggc cggcgttcca gcctgctgac cttgggggtg gatttggatt ggcggaggtt 540
gaattatatg gtgatgttgt gctccgtttt gtttcccatc cagatggtgc tgatgctcca 600
tttctacctg gatttgaagg ggtgtcaaac cccggcgcgg tggattatgg actgcgtcga 660
tttgatcacg tggtaggtaa tgttcctgag ttggctcccg tggcggccta catttcaggc 720
tttactggct tccatgaatt tgccgagttc accgcggaag atgtgggaac tgctgaaagt 780
ggattgaact ctgttgtcct ggcgaacaat gcagagactg tattgctccc cttgaatgag 840
cctgtgcacg gcaccaaacg gcgatctcaa attcagacat acctcgacca ccacgggggc 900
cctggtgttc agcatattgc tctagccagc gacgacgtac tgggaacact gagggagatg 960
agagcccgtt ccgccatggg tggctttgag tttctggccc ctccgccgcc aaactactac 1020
gacggcgtca gacggcgggc gggtgacgtc ttgagtgagg agcagatcaa tgaatgtcaa 1080
gaacttgggg ttcttgttga tagagatgat caaggagtgc tcctccaaat agccacaaaa 1140
cctgttggcg accgcccgac cttcttcctt gaaatgatcc aaaggatcgg ctgcatggag 1200
aaggatgaat cgggccagga gtaccagaag ggtgggtgtg gtggctttgg gaagggcaac 1260
ttcagcgagc tgttcaagtc aattgaagaa tatgaaaaat cattggaggc aaagcaagct 1320
ccgacggtgc agggaagctg a 1341
<210> 101
<211> 446
<212> PRT
<213> 人工序列-OsHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 101
Met Pro Pro Thr Pro Thr Pro Thr Ala Thr Thr Gly Ala Val Ser Ala
1 5 10 15
Ala Ala Ala Ala Gly Glu Asn Ala Gly Phe Arg Leu Val Gly His Arg
20 25 30
Arg Phe Val Arg Ala Asn Pro Arg Ser Asp Arg Phe Gln Ala Leu Ala
35 40 45
Phe His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly
50 55 60
Arg Phe Ala Phe Ala Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu
65 70 75 80
Ser Thr Gly Asn Ser Ala His Ala Ser Leu Leu Leu Arg Ser Ala Ser
85 90 95
Val Ala Phe Leu Phe Thr Ala Pro Tyr Gly Gly Asp His Gly Val Gly
100 105 110
Ala Asp Ala Ala Thr Thr Ala Ser Ile Pro Ser Phe Ser Pro Gly Ala
115 120 125
Ala Arg Arg Phe Ala Ala Asp His Gly Leu Ala Val His Ala Val Ala
130 135 140
Leu Arg Val Ala Asp Ala Ala Asp Ala Phe Arg Ala Ser Val Ala Ala
145 150 155 160
Gly Ala Arg Pro Ala Phe Gln Pro Ala Asp Leu Gly Gly Gly Phe Gly
165 170 175
Leu Ala Glu Val Glu Leu Tyr Gly Asp Val Val Leu Arg Phe Val Ser
180 185 190
His Pro Asp Gly Ala Asp Ala Pro Phe Leu Pro Gly Phe Glu Gly Val
195 200 205
Ser Asn Pro Gly Ala Val Asp Tyr Gly Leu Arg Arg Phe Asp His Val
210 215 220
Val Gly Asn Val Pro Glu Leu Ala Pro Val Ala Ala Tyr Ile Ser Gly
225 230 235 240
Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val Gly
245 250 255
Thr Ala Glu Ser Gly Leu Asn Ser Val Val Leu Ala Asn Asn Ala Glu
260 265 270
Thr Val Leu Leu Pro Leu Asn Glu Pro Val His Gly Thr Lys Arg Arg
275 280 285
Ser Gln Ile Gln Thr Tyr Leu Asp His His Gly Gly Pro Gly Val Gln
290 295 300
His Ile Ala Leu Ala Ser Asp Asp Val Leu Gly Thr Leu Arg Glu Met
305 310 315 320
Arg Ala Arg Ser Ala Met Gly Gly Phe Glu Phe Leu Ala Pro Pro Pro
325 330 335
Pro Asn Tyr Tyr Asp Gly Val Arg Arg Arg Ala Gly Asp Val Leu Ser
340 345 350
Glu Glu Gln Ile Asn Glu Cys Gln Glu Leu Gly Val Leu Val Asp Arg
355 360 365
Asp Asp Gln Gly Val Leu Leu Gln Ile Phe Thr Lys Pro Val Gly Asp
370 375 380
Arg Pro Thr Phe Trp Leu Glu Met Ile Gln Arg Ile Gly Cys Met Glu
385 390 395 400
Lys Asp Glu Ser Gly Gln Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe
405 410 415
Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu
420 425 430
Lys Ser Leu Glu Ala Lys Gln Ala Pro Thr Val Gln Gly Ser
435 440 445
<210> 102
<211> 1341
<212> DNA
<213> 人工序列-OsHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 102
atgcctccca ctcccacccc caccgccacc accggcgccg tctcggccgc tgcggcggcg 60
ggggagaacg cggggttccg cctcgtcggg caccgccgct tcgtccgcgc caacccgcgg 120
agcgaccggt tccaggcgct cgcgttccac cacgtcgagc tctggtgcgc cgacgccgcg 180
tccgccgcgg gccggttcgc cttcgccctg ggcgcgccgc tcgccgccag gtccgacctc 240
tccacgggga actccgcgca cgcctccctc ctcctccgct ccgcctccgt cgcgttcctc 300
ttcaccgccc cctacggcgg cgaccacggc gtcggcgcgg acgcggccac caccgcctcc 360
atcccttcct tctccccagg cgccgcgcgg aggttcgccg cggaccacgg cctcgcggtg 420
cacgccgtgg cgctgcgcgt cgccgacgcg gccgacgcct tccgcgccag cgtcgcggcc 480
ggtgcgcgcc cggcgttcca gcccgccgac ctcggcggtg gcttcggcct cgcggaggtg 540
gagctctacg gcgacgtcgt gctccgcttc gtcagccacc cggacggcgc cgacgcgccc 600
ttcctcccgg gtttcgaggg cgtcagcaac ccgggcgccg tggactacgg cctccgccgg 660
ttcgaccacg tcgtcggcaa cgtgccggag ctcgctccgg tagccgcgta catctccggg 720
ttcaccgggt tccacgagtt cgccgagttc accgccgagg acgtgggcac cgccgagagc 780
ggcctcaact cggtggtgct cgccaacaac gcggagaccg tgctgctgcc gctcaacgag 840
ccggtgcacg gcaccaagcg gcggagccag atacagacgt acctggacca ccacggcggc 900
ccgggggtgc agcacatcgc gctggccagc gacgacgtgc tcgggacgct gagggagatg 960
cgggcgcgct ccgccatggg cggcttcgag ttcttggcgc cgccgccgcc caactactac 1020
gacggcgtgc ggcggcgcgc cggggacgtg ctctcggagg agcagatcaa cgagtgccag 1080
gagctcgggg tgctcgtgga cagggatgac cagggggtgt tgctccagat cttcaccaag 1140
ccagtaggag acaggccaac cttttggttg gagatgatac aaaggattgg gtgcatggag 1200
aaggatgaga gtgggcagga gtaccagaag ggcggctgcg gcgggtttgg gaagggcaac 1260
ttctcggagc tgttcaagtc cattgaggag tatgagaaat cccttgaagc caagcaagcc 1320
cctacagttc aaggatccta g 1341
<210> 103
<211> 1341
<212> DNA
<213> 人工序列-OsHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 103
atgccaccca cgccaacacc aacggccacc actggtgccg tctcggccgc ggcagcggcc 60
ggtgagaatg ctggtttcag gctagtcggc caccgccgct tcgtgagggc aaatccaaga 120
tctgatcggt tccaagcact tgctttccac catgttgagc tgtggtgcgc tgacgccgct 180
tcagctgccg ggaggtttgc ttttgcttta ggggcgccgc tcgccgcaag gagtgatctt 240
tccaccggaa attctgctca tgcttctctt ctacttagat cagcaagtgt tgcatttctg 300
ttcactgcac cttatggcgg agaccatggt gtcggggccg atgctgcaac aacagctagc 360
ataccatcat tttctcccgg cgccgcaaga agatttgcag ccgatcatgg cctggcagtt 420
catgctgtgg cattgagggt cgccgacgcg gctgatgcgt tccgcgccag cgtggctgct 480
ggagctaggc cggcgttcca gcctgctgac cttgggggtg gatttggatt ggcggaggtt 540
gaattatatg gtgatgttgt gctccgtttt gtttcccatc cagatggtgc tgatgctcca 600
tttctacctg gatttgaagg ggtgtcaaac cccggcgcgg tggattatgg actgcgtcga 660
tttgatcacg tggtaggtaa tgttcctgag ttggctcccg tggcggccta catttcaggc 720
tttactggct tccatgaatt tgccgagttc accgcggaag atgtgggaac tgctgaaagt 780
ggattgaact ctgttgtcct ggcgaacaat gcagagactg tattgctccc cttgaatgag 840
cctgtgcacg gcaccaaacg gcgatctcaa attcagacat acctcgacca ccacgggggc 900
cctggtgttc agcatattgc tctagccagc gacgacgtac tgggaacact gagggagatg 960
agagcccgtt ccgccatggg tggctttgag tttctggccc ctccgccgcc aaactactac 1020
gacggcgtca gacggcgggc gggtgacgtc ttgagtgagg agcagatcaa tgaatgtcaa 1080
gaacttgggg ttcttgttga tagagatgat caaggagtgc tcctccaaat attcacaaaa 1140
cctgttggcg accgcccgac cttctggctt gaaatgatcc aaaggatcgg ctgcatggag 1200
aaggatgaat cgggccagga gtaccagaag ggtgggtgtg gtggctttgg gaagggcaac 1260
ttcagcgagc tgttcaagtc aattgaagaa tatgaaaaat cattggaggc aaagcaagct 1320
ccgacggtgc agggaagctg a 1341
<210> 104
<211> 446
<212> PRT
<213> 人工序列-OsHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 104
Met Pro Pro Thr Pro Thr Pro Thr Ala Thr Thr Gly Ala Val Ser Ala
1 5 10 15
Ala Ala Ala Ala Gly Glu Asn Ala Gly Phe Arg Leu Val Gly His Arg
20 25 30
Arg Phe Val Arg Ala Asn Pro Arg Ser Asp Arg Phe Gln Ala Leu Ala
35 40 45
Phe His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly
50 55 60
Arg Phe Ala Phe Ala Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu
65 70 75 80
Ser Thr Gly Asn Ser Ala His Ala Ser Leu Leu Leu Arg Ser Ala Ser
85 90 95
Val Ala Phe Leu Phe Thr Ala Pro Tyr Gly Gly Asp His Gly Val Gly
100 105 110
Ala Asp Ala Ala Thr Thr Ala Ser Ile Pro Ser Phe Ser Pro Gly Ala
115 120 125
Ala Arg Arg Phe Ala Ala Asp His Gly Leu Ala Val His Ala Val Ala
130 135 140
Leu Arg Val Ala Asp Ala Ala Asp Ala Phe Arg Ala Ser Val Ala Ala
145 150 155 160
Gly Ala Arg Pro Ala Phe Gln Pro Ala Asp Leu Gly Gly Gly Phe Gly
165 170 175
Leu Ala Glu Val Glu Leu Tyr Gly Asp Val Val Leu Arg Phe Val Ser
180 185 190
His Pro Asp Gly Ala Asp Ala Pro Phe Leu Pro Gly Phe Glu Gly Val
195 200 205
Ser Asn Pro Gly Ala Val Asp Tyr Gly Leu Arg Arg Phe Asp His Val
210 215 220
Val Gly Asn Val Pro Glu Leu Ala Pro Val Ala Ala Tyr Ile Ser Gly
225 230 235 240
Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala Glu Asp Val Gly
245 250 255
Thr Ala Glu Ser Gly Leu Asn Ser Val Val Leu Ala Asn Asn Ala Glu
260 265 270
Thr Val Leu Leu Pro Leu Asn Glu Pro Val His Gly Thr Lys Arg Arg
275 280 285
Ser Gln Ile Gln Thr Tyr Leu Asp His His Gly Gly Pro Gly Val Gln
290 295 300
His Ile Ala Leu Ala Ser Asp Asp Val Leu Gly Thr Leu Arg Glu Met
305 310 315 320
Arg Ala Arg Ser Ala Met Gly Gly Phe Glu Phe Leu Ala Pro Pro Pro
325 330 335
Pro Asn Tyr Tyr Asp Gly Val Arg Arg Arg Ala Gly Asp Val Leu Ser
340 345 350
Glu Glu Gln Ile Asn Glu Cys Gln Glu Leu Gly Val Leu Val Asp Arg
355 360 365
Asp Asp Gln Gly Val Leu Leu Gln Ile Ala Thr Lys Pro Val Gly Asp
370 375 380
Arg Pro Thr Phe Trp Leu Glu Met Ile Gln Arg Ile Gly Cys Met Glu
385 390 395 400
Lys Asp Glu Ser Gly Gln Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe
405 410 415
Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu
420 425 430
Lys Ser Leu Glu Ala Lys Gln Ala Pro Thr Val Gln Gly Ser
435 440 445
<210> 105
<211> 1341
<212> DNA
<213> 人工序列-OsHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 105
atgcctccca ctcccacccc caccgccacc accggcgccg tctcggccgc tgcggcggcg 60
ggggagaacg cggggttccg cctcgtcggg caccgccgct tcgtccgcgc caacccgcgg 120
agcgaccggt tccaggcgct cgcgttccac cacgtcgagc tctggtgcgc cgacgccgcg 180
tccgccgcgg gccggttcgc cttcgccctg ggcgcgccgc tcgccgccag gtccgacctc 240
tccacgggga actccgcgca cgcctccctc ctcctccgct ccgcctccgt cgcgttcctc 300
ttcaccgccc cctacggcgg cgaccacggc gtcggcgcgg acgcggccac caccgcctcc 360
atcccttcct tctccccagg cgccgcgcgg aggttcgccg cggaccacgg cctcgcggtg 420
cacgccgtgg cgctgcgcgt cgccgacgcg gccgacgcct tccgcgccag cgtcgcggcc 480
ggtgcgcgcc cggcgttcca gcccgccgac ctcggcggtg gcttcggcct cgcggaggtg 540
gagctctacg gcgacgtcgt gctccgcttc gtcagccacc cggacggcgc cgacgcgccc 600
ttcctcccgg gtttcgaggg cgtcagcaac ccgggcgccg tggactacgg cctccgccgg 660
ttcgaccacg tcgtcggcaa cgtgccggag ctcgctccgg tagccgcgta catctccggg 720
ttcaccgggt tccacgagtt cgccgagttc accgccgagg acgtgggcac cgccgagagc 780
ggcctcaact cggtggtgct cgccaacaac gcggagaccg tgctgctgcc gctcaacgag 840
ccggtgcacg gcaccaagcg gcggagccag atacagacgt acctggacca ccacggcggc 900
ccgggggtgc agcacatcgc gctggccagc gacgacgtgc tcgggacgct gagggagatg 960
cgggcgcgct ccgccatggg cggcttcgag ttcttggcgc cgccgccgcc caactactac 1020
gacggcgtgc ggcggcgcgc cggggacgtg ctctcggagg agcagatcaa cgagtgccag 1080
gagctcgggg tgctcgtgga cagggatgac cagggggtgt tgctccagat cgctaccaag 1140
ccagtaggag acaggccaac cttttggttg gagatgatac aaaggattgg gtgcatggag 1200
aaggatgaga gtgggcagga gtaccagaag ggcggctgcg gcgggtttgg gaagggcaac 1260
ttctcggagc tgttcaagtc cattgaggag tatgagaaat cccttgaagc caagcaagcc 1320
cctacagttc aaggatccta g 1341
<210> 106
<211> 1341
<212> DNA
<213> 人工序列-OsHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 106
atgccaccca cgccaacacc aacggccacc actggtgccg tctcggccgc ggcagcggcc 60
ggtgagaatg ctggtttcag gctagtcggc caccgccgct tcgtgagggc aaatccaaga 120
tctgatcggt tccaagcact tgctttccac catgttgagc tgtggtgcgc tgacgccgct 180
tcagctgccg ggaggtttgc ttttgcttta ggggcgccgc tcgccgcaag gagtgatctt 240
tccaccggaa attctgctca tgcttctctt ctacttagat cagcaagtgt tgcatttctg 300
ttcactgcac cttatggcgg agaccatggt gtcggggccg atgctgcaac aacagctagc 360
ataccatcat tttctcccgg cgccgcaaga agatttgcag ccgatcatgg cctggcagtt 420
catgctgtgg cattgagggt cgccgacgcg gctgatgcgt tccgcgccag cgtggctgct 480
ggagctaggc cggcgttcca gcctgctgac cttgggggtg gatttggatt ggcggaggtt 540
gaattatatg gtgatgttgt gctccgtttt gtttcccatc cagatggtgc tgatgctcca 600
tttctacctg gatttgaagg ggtgtcaaac cccggcgcgg tggattatgg actgcgtcga 660
tttgatcacg tggtaggtaa tgttcctgag ttggctcccg tggcggccta catttcaggc 720
tttactggct tccatgaatt tgccgagttc accgcggaag atgtgggaac tgctgaaagt 780
ggattgaact ctgttgtcct ggcgaacaat gcagagactg tattgctccc cttgaatgag 840
cctgtgcacg gcaccaaacg gcgatctcaa attcagacat acctcgacca ccacgggggc 900
cctggtgttc agcatattgc tctagccagc gacgacgtac tgggaacact gagggagatg 960
agagcccgtt ccgccatggg tggctttgag tttctggccc ctccgccgcc aaactactac 1020
gacggcgtca gacggcgggc gggtgacgtc ttgagtgagg agcagatcaa tgaatgtcaa 1080
gaacttgggg ttcttgttga tagagatgat caaggagtgc tcctccaaat agccacaaaa 1140
cctgttggcg accgcccgac cttctggctt gaaatgatcc aaaggatcgg ctgcatggag 1200
aaggatgaat cgggccagga gtaccagaag ggtgggtgtg gtggctttgg gaagggcaac 1260
ttcagcgagc tgttcaagtc aattgaagaa tatgaaaaat cattggaggc aaagcaagct 1320
ccgacggtgc agggaagctg a 1341
<210> 107
<211> 440
<212> PRT
<213> 高粱野生型HPPD(SbHPPD的氨基酸序列Sorghum bicolor)
<400> 107
Met Pro Pro Thr Pro Thr Thr Ala Ala Ala Thr Gly Ala Ala Val Ala
1 5 10 15
Ala Ala Ser Ala Glu Gln Ala Ala Phe Arg Leu Val Gly His Arg Asn
20 25 30
Phe Val Arg Val Asn Pro Arg Ser Asp Arg Phe His Thr Leu Ala Phe
35 40 45
His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg
50 55 60
Phe Ser Phe Gly Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Thr Ala His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu
85 90 95
Ala Phe Leu Phe Thr Ala Pro Tyr Ala His Gly Ala Asp Ala Ala Thr
100 105 110
Ala Ser Leu Pro Ser Phe Ser Ala Ala Glu Ala Arg Arg Phe Ala Ala
115 120 125
Asp His Gly Leu Ala Val Arg Ala Val Ala Leu Arg Val Ala Asp Ala
130 135 140
Glu Asp Ala Phe Arg Ala Ser Val Ala Ala Gly Ala Arg Pro Ala Phe
145 150 155 160
Glu Pro Val Glu Leu Gly Leu Gly Phe Arg Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Pro Asp Asp Ala Asp
180 185 190
Ala Ser Phe Leu Pro Gly Phe Val Gly Val Thr Ser Pro Gly Ala Ala
195 200 205
Asp Tyr Gly Leu Arg Arg Phe Asp His Ile Val Gly Asn Val Pro Glu
210 215 220
Leu Ala Pro Ala Ala Ala Tyr Phe Ala Gly Phe Thr Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Met Val Leu Ala Asn Asn Ala Glu Asn Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Asp His His Gly Gly Pro Gly Val Gln His Met Ala Leu Ala Ser
290 295 300
Asp Asp Val Leu Arg Thr Leu Arg Glu Met Gln Ala Arg Ser Ala Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Ala Pro Glu Tyr Tyr Asp Gly
325 330 335
Val Arg Arg Arg Ala Gly Asp Val Leu Thr Glu Ala Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Leu Phe Leu
370 375 380
Glu Ile Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Lys Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Gln Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Ala Lys
420 425 430
Gln Ala Ala Ala Ala Gln Gly Ser
435 440
<210> 108
<211> 1323
<212> DNA
<213> SbHPPD-01核苷酸序列(Sorghum bicolor)
<400> 108
atgcccccga cccccaccac agccgccgca accggcgccg ccgtggcggc ggcatcagcg 60
gagcaggcgg cgttccgcct cgtgggccac cgcaacttcg tccgcgtgaa cccgcgctcc 120
gaccgcttcc acacgctcgc gttccaccac gtggagctct ggtgcgccga cgcggcctcc 180
gccgcgggcc gcttctcctt cgggctcggc gcgccgctcg ccgcgcggtc cgacctctcc 240
acggggaaca ccgcgcacgc gtccctgctg ctccgctcgg gcgccctcgc gttcctcttc 300
acggcgccct acgcgcacgg cgccgacgcc gccacggcct cgctgccctc cttctccgcc 360
gccgaggcgc ggcgcttcgc ggccgaccac ggcctcgcgg tgcgcgccgt cgcgctccgc 420
gtggccgacg cggaggacgc cttccgcgcc agcgtcgcgg ccggcgcgcg cccggcgttc 480
gagcccgtcg agctcggcct cggcttccgc ctcgccgaag tcgagctcta cggcgacgtc 540
gtgctccggt acgtgagcta cccggacgac gcggacgcgt ccttcctgcc ggggttcgtg 600
ggcgtgacca gccccggcgc ggcggactac gggctgagga ggttcgacca catcgtcggc 660
aacgtgccgg agctggcgcc ggcggccgcc tacttcgctg gcttcacggg gttccacgag 720
ttcgccgagt tcacggcgga ggacgtgggc accacggaga gcgggctcaa ctcgatggtg 780
ctcgccaaca acgcggagaa cgtgctgctc ccactcaacg agccggtgca cggcaccaag 840
cgccgcagcc agatacagac gtacttggac caccacggcg gccccggcgt gcagcacatg 900
gcgctggcca gcgacgacgt gctcaggacg ctgagggaga tgcaagcgcg ctcggccatg 960
ggcggcttcg agttcatggc gcctccggcg cccgaatact atgacggcgt gaggcggcgc 1020
gccggggacg tgctcacgga ggcacagatt aaggagtgtc aggaactagg ggtgctggtg 1080
gacagagatg accagggcgt gctgctccag atcttcacca agccagtggg ggacaggcca 1140
acgttgttct tggagatcat tcaaaggatc gggtgcatgg agaaggatga gaaggggcaa 1200
gaataccaga agggtggctg tggcgggttt ggcaagggaa acttctccca gctgttcaaa 1260
tccattgagg attatgagaa gtcccttgaa gctaagcaag ctgcagcagc tcagggatcc 1320
tag 1323
<210> 109
<211> 1323
<212> DNA
<213> 人工序列-SbHPPD-02核苷酸序列(Artificial Sequence)
<400> 109
atgccgccca ctccaacaac tgctgctgcg acgggagcag ctgtcgccgc tgcttctgct 60
gagcaggctg ccttcaggct cgtcgggcac agaaattttg ttcgtgtaaa tccaaggagt 120
gataggttcc acaccttggc ttttcaccac gtggagttgt ggtgtgctga tgcagcctcg 180
gcagcggggc gcttctcgtt tggtctcggt gctccgctcg ccgcaagatc tgatcttagc 240
actggaaaca cagcgcatgc ctccctgctc ctgcgctctg gcgcgctggc gttcctcttc 300
acggcgccat atgctcacgg cgccgacgcc gccaccgcgt cattgccgtc ttttagtgca 360
gccgaagcaa gaagatttgc agcagaccac ggcctcgccg taagggccgt ggccttacgt 420
gtcgccgatg ccgaagatgc tttccgcgcc tctgtagcag ctggagcaag gccagcattt 480
gaacctgttg aattggggtt aggattccgt cttgcagaag ttgagcttta tggcgacgtt 540
gttttgagat acgtttcata tcctgatgac gctgacgcta gcttcttgcc tggttttgtg 600
ggggtgacat cacctggggc agcagattat ggtctcaggc ggtttgatca tattgttgga 660
aacgttcctg aactcgcgcc ggcggcagcc tacttcgccg gtttcactgg gtttcatgaa 720
tttgctgaat tcacagccga ggatgtcggc accaccgaga gtggactgaa ctccatggtg 780
ctggccaaca atgcagagaa tgtccttctt cccttgaatg aaccagttca tggaacaaaa 840
aggcgttccc aaatacaaac atacctcgac catcatggtg gaccaggcgt tcagcatatg 900
gctctcgcta gtgacgatgt tctacgaacc ctgcgagaga tgcaggcgcg gtctgcaatg 960
ggaggatttg agttcatggc acctcctgcc cctgaatatt atgatggtgt tcggagaagg 1020
gcaggtgatg tgctaactga agctcaaatc aaggagtgcc aggagcttgg cgtgctggtc 1080
gatcgggatg atcaaggtgt gcttctgcag atttttacta aaccagtggg tgaccgcccc 1140
acgctgttcc tagagatcat ccagagaata gggtgcatgg agaaggacga aaaaggacaa 1200
gagtaccaga agggaggttg tggcggcttt ggaaaaggga acttctccca attattcaag 1260
tcaattgaag actatgaaaa gagtttggag gctaagcaag ctgcagccgc ccagggcagc 1320
tga 1323
<210> 110
<211> 440
<212> PRT
<213> 人工序列-SbHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 110
Met Pro Pro Thr Pro Thr Thr Ala Ala Ala Thr Gly Ala Ala Val Ala
1 5 10 15
Ala Ala Ser Ala Glu Gln Ala Ala Phe Arg Leu Val Gly His Arg Asn
20 25 30
Phe Val Arg Val Asn Pro Arg Ser Asp Arg Phe His Thr Leu Ala Phe
35 40 45
His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg
50 55 60
Phe Ser Phe Gly Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Thr Ala His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu
85 90 95
Ala Phe Leu Phe Thr Ala Pro Tyr Ala His Gly Ala Asp Ala Ala Thr
100 105 110
Ala Ser Leu Pro Ser Phe Ser Ala Ala Glu Ala Arg Arg Phe Ala Ala
115 120 125
Asp His Gly Leu Ala Val Arg Ala Val Ala Leu Arg Val Ala Asp Ala
130 135 140
Glu Asp Ala Phe Arg Ala Ser Val Ala Ala Gly Ala Arg Pro Ala Phe
145 150 155 160
Glu Pro Val Glu Leu Gly Leu Gly Phe Arg Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Pro Asp Asp Ala Asp
180 185 190
Ala Ser Phe Leu Pro Gly Phe Val Gly Val Thr Ser Pro Gly Ala Ala
195 200 205
Asp Tyr Gly Leu Arg Arg Phe Asp His Ile Val Gly Asn Val Pro Glu
210 215 220
Leu Ala Pro Ala Ala Ala Tyr Phe Ala Gly Phe Thr Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Met Val Leu Ala Asn Asn Ala Glu Asn Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Asp His His Gly Gly Pro Gly Val Gln His Met Ala Leu Ala Ser
290 295 300
Asp Asp Val Leu Arg Thr Leu Arg Glu Met Gln Ala Arg Ser Ala Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Ala Pro Glu Tyr Tyr Asp Gly
325 330 335
Val Arg Arg Arg Ala Gly Asp Val Leu Thr Glu Ala Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Leu Phe Leu
370 375 380
Glu Ile Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Lys Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Gln Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Ala Lys
420 425 430
Gln Ala Ala Ala Ala Gln Gly Ser
435 440
<210> 111
<211> 1323
<212> DNA
<213> 人工序列-SbHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 111
atgcccccga cccccaccac agccgccgca accggcgccg ccgtggcggc ggcatcagcg 60
gagcaggcgg cgttccgcct cgtgggccac cgcaacttcg tccgcgtgaa cccgcgctcc 120
gaccgcttcc acacgctcgc gttccaccac gtggagctct ggtgcgccga cgcggcctcc 180
gccgcgggcc gcttctcctt cgggctcggc gcgccgctcg ccgcgcggtc cgacctctcc 240
acggggaaca ccgcgcacgc gtccctgctg ctccgctcgg gcgccctcgc gttcctcttc 300
acggcgccct acgcgcacgg cgccgacgcc gccacggcct cgctgccctc cttctccgcc 360
gccgaggcgc ggcgcttcgc ggccgaccac ggcctcgcgg tgcgcgccgt cgcgctccgc 420
gtggccgacg cggaggacgc cttccgcgcc agcgtcgcgg ccggcgcgcg cccggcgttc 480
gagcccgtcg agctcggcct cggcttccgc ctcgccgaag tcgagctcta cggcgacgtc 540
gtgctccggt acgtgagcta cccggacgac gcggacgcgt ccttcctgcc ggggttcgtg 600
ggcgtgacca gccccggcgc ggcggactac gggctgagga ggttcgacca catcgtcggc 660
aacgtgccgg agctggcgcc ggcggccgcc tacttcgctg gcttcacggg gttccacgag 720
ttcgccgagt tcacggcgga ggacgtgggc accacggaga gcgggctcaa ctcgatggtg 780
ctcgccaaca acgcggagaa cgtgctgctc ccactcaacg agccggtgca cggcaccaag 840
cgccgcagcc agatacagac gtacttggac caccacggcg gccccggcgt gcagcacatg 900
gcgctggcca gcgacgacgt gctcaggacg ctgagggaga tgcaagcgcg ctcggccatg 960
ggcggcttcg agttcatggc gcctccggcg cccgaatact atgacggcgt gaggcggcgc 1020
gccggggacg tgctcacgga ggcacagatt aaggagtgtc aggaactagg ggtgctggtg 1080
gacagagatg accagggcgt gctgctccag atcgctacca agccagtggg ggacaggcca 1140
acgttgttct tggagatcat tcaaaggatc gggtgcatgg agaaggatga gaaggggcaa 1200
gaataccaga agggtggctg tggcgggttt ggcaagggaa acttctccca gctgttcaaa 1260
tccattgagg attatgagaa gtcccttgaa gctaagcaag ctgcagcagc tcagggatcc 1320
tag 1323
<210> 112
<211> 1323
<212> DNA
<213> 人工序列-SbHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 112
atgccgccca ctccaacaac tgctgctgcg acgggagcag ctgtcgccgc tgcttctgct 60
gagcaggctg ccttcaggct cgtcgggcac agaaattttg ttcgtgtaaa tccaaggagt 120
gataggttcc acaccttggc ttttcaccac gtggagttgt ggtgtgctga tgcagcctcg 180
gcagcggggc gcttctcgtt tggtctcggt gctccgctcg ccgcaagatc tgatcttagc 240
actggaaaca cagcgcatgc ctccctgctc ctgcgctctg gcgcgctggc gttcctcttc 300
acggcgccat atgctcacgg cgccgacgcc gccaccgcgt cattgccgtc ttttagtgca 360
gccgaagcaa gaagatttgc agcagaccac ggcctcgccg taagggccgt ggccttacgt 420
gtcgccgatg ccgaagatgc tttccgcgcc tctgtagcag ctggagcaag gccagcattt 480
gaacctgttg aattggggtt aggattccgt cttgcagaag ttgagcttta tggcgacgtt 540
gttttgagat acgtttcata tcctgatgac gctgacgcta gcttcttgcc tggttttgtg 600
ggggtgacat cacctggggc agcagattat ggtctcaggc ggtttgatca tattgttgga 660
aacgttcctg aactcgcgcc ggcggcagcc tacttcgccg gtttcactgg gtttcatgaa 720
tttgctgaat tcacagccga ggatgtcggc accaccgaga gtggactgaa ctccatggtg 780
ctggccaaca atgcagagaa tgtccttctt cccttgaatg aaccagttca tggaacaaaa 840
aggcgttccc aaatacaaac atacctcgac catcatggtg gaccaggcgt tcagcatatg 900
gctctcgcta gtgacgatgt tctacgaacc ctgcgagaga tgcaggcgcg gtctgcaatg 960
ggaggatttg agttcatggc acctcctgcc cctgaatatt atgatggtgt tcggagaagg 1020
gcaggtgatg tgctaactga agctcaaatc aaggagtgcc aggagcttgg cgtgctggtc 1080
gatcgggatg atcaaggtgt gcttctgcag attgccacta aaccagtggg tgaccgcccc 1140
acgctgttcc tagagatcat ccagagaata gggtgcatgg agaaggacga aaaaggacaa 1200
gagtaccaga agggaggttg tggcggcttt ggaaaaggga acttctccca attattcaag 1260
tcaattgaag actatgaaaa gagtttggag gctaagcaag ctgcagccgc ccagggcagc 1320
tga 1323
<210> 113
<211> 440
<212> PRT
<213> 人工序列-SbHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 113
Met Pro Pro Thr Pro Thr Thr Ala Ala Ala Thr Gly Ala Ala Val Ala
1 5 10 15
Ala Ala Ser Ala Glu Gln Ala Ala Phe Arg Leu Val Gly His Arg Asn
20 25 30
Phe Val Arg Val Asn Pro Arg Ser Asp Arg Phe His Thr Leu Ala Phe
35 40 45
His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg
50 55 60
Phe Ser Phe Gly Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Thr Ala His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu
85 90 95
Ala Phe Leu Phe Thr Ala Pro Tyr Ala His Gly Ala Asp Ala Ala Thr
100 105 110
Ala Ser Leu Pro Ser Phe Ser Ala Ala Glu Ala Arg Arg Phe Ala Ala
115 120 125
Asp His Gly Leu Ala Val Arg Ala Val Ala Leu Arg Val Ala Asp Ala
130 135 140
Glu Asp Ala Phe Arg Ala Ser Val Ala Ala Gly Ala Arg Pro Ala Phe
145 150 155 160
Glu Pro Val Glu Leu Gly Leu Gly Phe Arg Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Pro Asp Asp Ala Asp
180 185 190
Ala Ser Phe Leu Pro Gly Phe Val Gly Val Thr Ser Pro Gly Ala Ala
195 200 205
Asp Tyr Gly Leu Arg Arg Phe Asp His Ile Val Gly Asn Val Pro Glu
210 215 220
Leu Ala Pro Ala Ala Ala Tyr Phe Ala Gly Phe Thr Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Met Val Leu Ala Asn Asn Ala Glu Asn Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Asp His His Gly Gly Pro Gly Val Gln His Met Ala Leu Ala Ser
290 295 300
Asp Asp Val Leu Arg Thr Leu Arg Glu Met Gln Ala Arg Ser Ala Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Ala Pro Glu Tyr Tyr Asp Gly
325 330 335
Val Arg Arg Arg Ala Gly Asp Val Leu Thr Glu Ala Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Leu Trp Leu
370 375 380
Glu Ile Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Lys Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Gln Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Ala Lys
420 425 430
Gln Ala Ala Ala Ala Gln Gly Ser
435 440
<210> 114
<211> 1323
<212> DNA
<213> 人工序列-SbHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 114
atgcccccga cccccaccac agccgccgca accggcgccg ccgtggcggc ggcatcagcg 60
gagcaggcgg cgttccgcct cgtgggccac cgcaacttcg tccgcgtgaa cccgcgctcc 120
gaccgcttcc acacgctcgc gttccaccac gtggagctct ggtgcgccga cgcggcctcc 180
gccgcgggcc gcttctcctt cgggctcggc gcgccgctcg ccgcgcggtc cgacctctcc 240
acggggaaca ccgcgcacgc gtccctgctg ctccgctcgg gcgccctcgc gttcctcttc 300
acggcgccct acgcgcacgg cgccgacgcc gccacggcct cgctgccctc cttctccgcc 360
gccgaggcgc ggcgcttcgc ggccgaccac ggcctcgcgg tgcgcgccgt cgcgctccgc 420
gtggccgacg cggaggacgc cttccgcgcc agcgtcgcgg ccggcgcgcg cccggcgttc 480
gagcccgtcg agctcggcct cggcttccgc ctcgccgaag tcgagctcta cggcgacgtc 540
gtgctccggt acgtgagcta cccggacgac gcggacgcgt ccttcctgcc ggggttcgtg 600
ggcgtgacca gccccggcgc ggcggactac gggctgagga ggttcgacca catcgtcggc 660
aacgtgccgg agctggcgcc ggcggccgcc tacttcgctg gcttcacggg gttccacgag 720
ttcgccgagt tcacggcgga ggacgtgggc accacggaga gcgggctcaa ctcgatggtg 780
ctcgccaaca acgcggagaa cgtgctgctc ccactcaacg agccggtgca cggcaccaag 840
cgccgcagcc agatacagac gtacttggac caccacggcg gccccggcgt gcagcacatg 900
gcgctggcca gcgacgacgt gctcaggacg ctgagggaga tgcaagcgcg ctcggccatg 960
ggcggcttcg agttcatggc gcctccggcg cccgaatact atgacggcgt gaggcggcgc 1020
gccggggacg tgctcacgga ggcacagatt aaggagtgtc aggaactagg ggtgctggtg 1080
gacagagatg accagggcgt gctgctccag atcttcacca agccagtggg ggacaggcca 1140
acgttgtggt tggagatcat tcaaaggatc gggtgcatgg agaaggatga gaaggggcaa 1200
gaataccaga agggtggctg tggcgggttt ggcaagggaa acttctccca gctgttcaaa 1260
tccattgagg attatgagaa gtcccttgaa gctaagcaag ctgcagcagc tcagggatcc 1320
tag 1323
<210> 115
<211> 1323
<212> DNA
<213> 人工序列-SbHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 115
atgccgccca ctccaacaac tgctgctgcg acgggagcag ctgtcgccgc tgcttctgct 60
gagcaggctg ccttcaggct cgtcgggcac agaaattttg ttcgtgtaaa tccaaggagt 120
gataggttcc acaccttggc ttttcaccac gtggagttgt ggtgtgctga tgcagcctcg 180
gcagcggggc gcttctcgtt tggtctcggt gctccgctcg ccgcaagatc tgatcttagc 240
actggaaaca cagcgcatgc ctccctgctc ctgcgctctg gcgcgctggc gttcctcttc 300
acggcgccat atgctcacgg cgccgacgcc gccaccgcgt cattgccgtc ttttagtgca 360
gccgaagcaa gaagatttgc agcagaccac ggcctcgccg taagggccgt ggccttacgt 420
gtcgccgatg ccgaagatgc tttccgcgcc tctgtagcag ctggagcaag gccagcattt 480
gaacctgttg aattggggtt aggattccgt cttgcagaag ttgagcttta tggcgacgtt 540
gttttgagat acgtttcata tcctgatgac gctgacgcta gcttcttgcc tggttttgtg 600
ggggtgacat cacctggggc agcagattat ggtctcaggc ggtttgatca tattgttgga 660
aacgttcctg aactcgcgcc ggcggcagcc tacttcgccg gtttcactgg gtttcatgaa 720
tttgctgaat tcacagccga ggatgtcggc accaccgaga gtggactgaa ctccatggtg 780
ctggccaaca atgcagagaa tgtccttctt cccttgaatg aaccagttca tggaacaaaa 840
aggcgttccc aaatacaaac atacctcgac catcatggtg gaccaggcgt tcagcatatg 900
gctctcgcta gtgacgatgt tctacgaacc ctgcgagaga tgcaggcgcg gtctgcaatg 960
ggaggatttg agttcatggc acctcctgcc cctgaatatt atgatggtgt tcggagaagg 1020
gcaggtgatg tgctaactga agctcaaatc aaggagtgcc aggagcttgg cgtgctggtc 1080
gatcgggatg atcaaggtgt gcttctgcag atttttacta aaccagtggg tgaccgcccc 1140
acgctgtggc tagagatcat ccagagaata gggtgcatgg agaaggacga aaaaggacaa 1200
gagtaccaga agggaggttg tggcggcttt ggaaaaggga acttctccca attattcaag 1260
tcaattgaag actatgaaaa gagtttggag gctaagcaag ctgcagccgc ccagggcagc 1320
tga 1323
<210> 116
<211> 440
<212> PRT
<213> 人工序列-SbHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 116
Met Pro Pro Thr Pro Thr Thr Ala Ala Ala Thr Gly Ala Ala Val Ala
1 5 10 15
Ala Ala Ser Ala Glu Gln Ala Ala Phe Arg Leu Val Gly His Arg Asn
20 25 30
Phe Val Arg Val Asn Pro Arg Ser Asp Arg Phe His Thr Leu Ala Phe
35 40 45
His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg
50 55 60
Phe Ser Phe Gly Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Thr Ala His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu
85 90 95
Ala Phe Leu Phe Thr Ala Pro Tyr Ala His Gly Ala Asp Ala Ala Thr
100 105 110
Ala Ser Leu Pro Ser Phe Ser Ala Ala Glu Ala Arg Arg Phe Ala Ala
115 120 125
Asp His Gly Leu Ala Val Arg Ala Val Ala Leu Arg Val Ala Asp Ala
130 135 140
Glu Asp Ala Phe Arg Ala Ser Val Ala Ala Gly Ala Arg Pro Ala Phe
145 150 155 160
Glu Pro Val Glu Leu Gly Leu Gly Phe Arg Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Pro Asp Asp Ala Asp
180 185 190
Ala Ser Phe Leu Pro Gly Phe Val Gly Val Thr Ser Pro Gly Ala Ala
195 200 205
Asp Tyr Gly Leu Arg Arg Phe Asp His Ile Val Gly Asn Val Pro Glu
210 215 220
Leu Ala Pro Ala Ala Ala Tyr Phe Ala Gly Phe Thr Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Met Val Leu Ala Asn Asn Ala Glu Asn Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Asp His His Gly Gly Pro Gly Val Gln His Met Ala Leu Ala Ser
290 295 300
Asp Asp Val Leu Arg Thr Leu Arg Glu Met Gln Ala Arg Ser Ala Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Ala Pro Glu Tyr Tyr Asp Gly
325 330 335
Val Arg Arg Arg Ala Gly Asp Val Leu Thr Glu Ala Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Leu Trp Leu
370 375 380
Glu Ile Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Lys Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Gln Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Ala Lys
420 425 430
Gln Ala Ala Ala Ala Gln Gly Ser
435 440
<210> 117
<211> 1323
<212> DNA
<213> 人工序列-SbHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 117
atgcccccga cccccaccac agccgccgca accggcgccg ccgtggcggc ggcatcagcg 60
gagcaggcgg cgttccgcct cgtgggccac cgcaacttcg tccgcgtgaa cccgcgctcc 120
gaccgcttcc acacgctcgc gttccaccac gtggagctct ggtgcgccga cgcggcctcc 180
gccgcgggcc gcttctcctt cgggctcggc gcgccgctcg ccgcgcggtc cgacctctcc 240
acggggaaca ccgcgcacgc gtccctgctg ctccgctcgg gcgccctcgc gttcctcttc 300
acggcgccct acgcgcacgg cgccgacgcc gccacggcct cgctgccctc cttctccgcc 360
gccgaggcgc ggcgcttcgc ggccgaccac ggcctcgcgg tgcgcgccgt cgcgctccgc 420
gtggccgacg cggaggacgc cttccgcgcc agcgtcgcgg ccggcgcgcg cccggcgttc 480
gagcccgtcg agctcggcct cggcttccgc ctcgccgaag tcgagctcta cggcgacgtc 540
gtgctccggt acgtgagcta cccggacgac gcggacgcgt ccttcctgcc ggggttcgtg 600
ggcgtgacca gccccggcgc ggcggactac gggctgagga ggttcgacca catcgtcggc 660
aacgtgccgg agctggcgcc ggcggccgcc tacttcgctg gcttcacggg gttccacgag 720
ttcgccgagt tcacggcgga ggacgtgggc accacggaga gcgggctcaa ctcgatggtg 780
ctcgccaaca acgcggagaa cgtgctgctc ccactcaacg agccggtgca cggcaccaag 840
cgccgcagcc agatacagac gtacttggac caccacggcg gccccggcgt gcagcacatg 900
gcgctggcca gcgacgacgt gctcaggacg ctgagggaga tgcaagcgcg ctcggccatg 960
ggcggcttcg agttcatggc gcctccggcg cccgaatact atgacggcgt gaggcggcgc 1020
gccggggacg tgctcacgga ggcacagatt aaggagtgtc aggaactagg ggtgctggtg 1080
gacagagatg accagggcgt gctgctccag atcgctacca agccagtggg ggacaggcca 1140
acgttgtggt tggagatcat tcaaaggatc gggtgcatgg agaaggatga gaaggggcaa 1200
gaataccaga agggtggctg tggcgggttt ggcaagggaa acttctccca gctgttcaaa 1260
tccattgagg attatgagaa gtcccttgaa gctaagcaag ctgcagcagc tcagggatcc 1320
tag 1323
<210> 118
<211> 1323
<212> DNA
<213> 人工序列-SbHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 118
atgccgccca ctccaacaac tgctgctgcg acgggagcag ctgtcgccgc tgcttctgct 60
gagcaggctg ccttcaggct cgtcgggcac agaaattttg ttcgtgtaaa tccaaggagt 120
gataggttcc acaccttggc ttttcaccac gtggagttgt ggtgtgctga tgcagcctcg 180
gcagcggggc gcttctcgtt tggtctcggt gctccgctcg ccgcaagatc tgatcttagc 240
actggaaaca cagcgcatgc ctccctgctc ctgcgctctg gcgcgctggc gttcctcttc 300
acggcgccat atgctcacgg cgccgacgcc gccaccgcgt cattgccgtc ttttagtgca 360
gccgaagcaa gaagatttgc agcagaccac ggcctcgccg taagggccgt ggccttacgt 420
gtcgccgatg ccgaagatgc tttccgcgcc tctgtagcag ctggagcaag gccagcattt 480
gaacctgttg aattggggtt aggattccgt cttgcagaag ttgagcttta tggcgacgtt 540
gttttgagat acgtttcata tcctgatgac gctgacgcta gcttcttgcc tggttttgtg 600
ggggtgacat cacctggggc agcagattat ggtctcaggc ggtttgatca tattgttgga 660
aacgttcctg aactcgcgcc ggcggcagcc tacttcgccg gtttcactgg gtttcatgaa 720
tttgctgaat tcacagccga ggatgtcggc accaccgaga gtggactgaa ctccatggtg 780
ctggccaaca atgcagagaa tgtccttctt cccttgaatg aaccagttca tggaacaaaa 840
aggcgttccc aaatacaaac atacctcgac catcatggtg gaccaggcgt tcagcatatg 900
gctctcgcta gtgacgatgt tctacgaacc ctgcgagaga tgcaggcgcg gtctgcaatg 960
ggaggatttg agttcatggc acctcctgcc cctgaatatt atgatggtgt tcggagaagg 1020
gcaggtgatg tgctaactga agctcaaatc aaggagtgcc aggagcttgg cgtgctggtc 1080
gatcgggatg atcaaggtgt gcttctgcag attgccacta aaccagtggg tgaccgcccc 1140
acgctgtggc tagagatcat ccagagaata gggtgcatgg agaaggacga aaaaggacaa 1200
gagtaccaga agggaggttg tggcggcttt ggaaaaggga acttctccca attattcaag 1260
tcaattgaag actatgaaaa gagtttggag gctaagcaag ctgcagccgc ccagggcagc 1320
tga 1323
<210> 119
<211> 434
<212> PRT
<213> 大麦野生型HPPD(HvHPPD的氨基酸序列Hordeum vulgare)
<400> 119
Met Pro Pro Thr Pro Thr Thr Pro Ala Ala Thr Gly Ala Ala Ala Ala
1 5 10 15
Val Thr Pro Glu His Ala Arg Pro His Arg Met Val Arg Phe Asn Pro
20 25 30
Arg Ser Asp Arg Phe His Thr Leu Ser Phe His His Val Glu Phe Trp
35 40 45
Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ala Phe Ala Leu Gly
50 55 60
Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala His
65 70 75 80
Ala Ser Gln Leu Leu Arg Ser Gly Ser Leu Ala Phe Leu Phe Thr Ala
85 90 95
Pro Tyr Ala Asn Gly Cys Asp Ala Ala Thr Ala Ser Leu Pro Ser Phe
100 105 110
Ser Ala Asp Ala Ala Arg Arg Phe Ser Ala Asp His Gly Ile Ala Val
115 120 125
Arg Ser Val Ala Leu Arg Val Ala Asp Ala Ala Glu Ala Phe Arg Ala
130 135 140
Ser Arg Arg Arg Gly Ala Arg Pro Ala Phe Ala Pro Val Asp Leu Gly
145 150 155 160
Arg Gly Phe Ala Phe Ala Glu Val Glu Leu Tyr Gly Asp Val Val Leu
165 170 175
Arg Phe Val Ser His Pro Asp Gly Thr Asp Val Pro Phe Leu Pro Gly
180 185 190
Phe Glu Gly Val Thr Asn Pro Asp Ala Val Asp Tyr Gly Leu Thr Arg
195 200 205
Phe Asp His Val Val Gly Asn Val Pro Glu Leu Ala Pro Ala Ala Ala
210 215 220
Tyr Ile Ala Gly Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala
225 230 235 240
Glu Asp Val Gly Thr Thr Glu Ser Gly Leu Asn Ser Val Val Leu Ala
245 250 255
Asn Asn Ser Glu Gly Val Leu Leu Pro Leu Asn Glu Pro Val His Gly
260 265 270
Thr Lys Arg Arg Ser Gln Ile Gln Thr Phe Leu Glu His His Gly Gly
275 280 285
Pro Gly Val Gln His Ile Ala Val Ala Ser Ser Asp Val Leu Arg Thr
290 295 300
Leu Arg Lys Met Arg Ala Arg Ser Ala Met Gly Gly Phe Asp Phe Leu
305 310 315 320
Pro Pro Pro Leu Pro Lys Tyr Tyr Glu Gly Val Arg Arg Leu Ala Gly
325 330 335
Asp Val Leu Ser Glu Ala Gln Ile Lys Glu Cys Gln Glu Leu Gly Val
340 345 350
Leu Val Asp Arg Asp Asp Gln Gly Val Leu Leu Gln Ile Phe Thr Lys
355 360 365
Pro Val Gly Asp Arg Pro Thr Leu Phe Leu Glu Met Ile Gln Arg Ile
370 375 380
Gly Cys Met Glu Lys Asp Glu Arg Gly Glu Glu Tyr Gln Lys Gly Gly
385 390 395 400
Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile
405 410 415
Glu Asp Tyr Glu Lys Ser Leu Glu Ala Lys Gln Ser Ala Ala Val Gln
420 425 430
Gly Ser
<210> 120
<211> 1305
<212> DNA
<213> HvHPPD-01核苷酸序列(Hordeum vulgare)
<400> 120
atgccgccca cccccaccac ccccgcggct accggcgccg ccgccgcggt gacgccggag 60
cacgcgcgac cgcaccgaat ggtccgcttc aacccgcgca gcgaccgctt ccacacgctc 120
tccttccacc acgtcgagtt ctggtgcgcg gacgccgcct ccgccgccgg ccgcttcgcg 180
ttcgcgctcg gcgcgccgct cgccgccagg tccgacctct ccacggggaa ctccgcgcac 240
gcctcccagc tgctccgctc gggctccctc gccttcctct tcaccgcgcc ctacgccaac 300
ggctgcgacg ccgccaccgc ctccctgccc tccttctccg ccgacgccgc gcgccggttc 360
tccgccgacc acgggatcgc ggtgcgctcc gtagcgctgc gcgtcgcaga cgccgccgag 420
gccttccgcg ccagtcgtcg acggggcgcg cgcccggcct tcgcccccgt ggacctcggc 480
cgcggcttcg cgttcgcgga ggtcgagctc tacggcgacg tcgtgctccg cttcgtcagc 540
cacccggacg gcacggacgt gcccttcttg ccggggttcg agggcgtaac caacccggac 600
gccgtggact acggcctgac gcggttcgac cacgtcgtcg gcaacgtccc ggagcttgcc 660
cccgccgcag cctacatcgc cgggttcacg gggttccacg agttcgccga gttcacggcg 720
gaggacgtgg gcacgaccga gagcgggctc aactcggtgg tgctcgccaa caactcggag 780
ggcgtgctgc tgccgctcaa cgagccggtg cacggcacca agcgccggag ccagatacag 840
acgttcctgg aacaccacgg cggcccgggc gtgcagcaca tcgcggtggc cagcagtgac 900
gtgctcagga cgctcaggaa gatgcgtgcg cgctccgcca tgggcggctt cgacttcctg 960
ccacccccgc tgccgaagta ctacgaaggc gtgcgacgcc ttgccgggga tgtcctctcg 1020
gaggcgcaga tcaaggaatg ccaggagctg ggtgtgctcg tcgataggga cgaccaaggg 1080
gtgttgctcc aaatcttcac caagccagta ggggacaggc cgaccttgtt cctggagatg 1140
atccagagga tcgggtgcat ggagaaggac gagagagggg aagagtacca gaagggtggc 1200
tgcggcgggt tcggcaaagg caacttctcc gagctgttca agtccattga agattacgag 1260
aagtcccttg aagccaagca atctgctgca gttcagggat catag 1305
<210> 121
<211> 1305
<212> DNA
<213> 人工序列-HvHPPD-02核苷酸序列(Artificial Sequence)
<400> 121
atgccgccga cgcccaccac cccagcagct accggagctg cggctgcagt aacccctgag 60
catgctcggc cccacaggat ggtgaggttt aatccaagat cagatcgttt tcatacactc 120
tcgttccacc atgttgagtt ctggtgcgcc gacgcggctt cagcggccgg gaggttcgcc 180
ttcgcgctgg gggcccctct tgccgcgcgt agtgatttgt ctactgggaa ttcagcgcac 240
gcgtcccagc ttctccgaag cggcagtctc gcctttctct tcactgcacc atatgcaaat 300
ggatgtgatg cagcaactgc ttccctgccg agcttttctg cggatgccgc gaggcgattc 360
agtgctgacc atgggatcgc cgtccggtca gttgctctta gagtggctga tgctgctgag 420
gcattcaggg cttcaaggcg tcgtggtgca cgcccggcgt ttgccccggt tgatcttggg 480
cgggggtttg cttttgcaga ggtggagctg tacggcgacg tggtgctccg atttgtttct 540
cacccggacg gcactgatgt gcccttcttg ccaggatttg aaggtgtgac caaccctgat 600
gccgtggatt atggactcac aagatttgat catgtggttg gcaatgttcc tgaattggca 660
cctgctgccg cttacatcgc aggttttact ggcttccatg aatttgctga attcacggcg 720
gaggatgttg gtacaactga gtcagggctg aacagtgttg ttttagccaa caactcagaa 780
ggagttctcc ttcccttgaa tgaacctgtt catggaacca agcgccgctc ccaaattcaa 840
acatttcttg agcaccacgg cggccctggt gttcaacata ttgctgtcgc ctctagcgac 900
gtcctgcgta cattacgcaa gatgagggct cggtccgcaa tgggtggttt cgacttcctc 960
cctccaccat tgccaaaata ttatgaaggc gtcagaagac ttgcaggaga tgtcctctct 1020
gaagctcaga tcaaggagtg ccaagaactt ggcgtcttgg ttgatagaga tgaccaagga 1080
gtacttctgc agatatttac aaaacccgtt ggtgacaggc caactctttt cctggagatg 1140
attcagagaa ttggctgcat ggagaaagat gaaagaggcg aggagtacca gaagggtggt 1200
tgtggaggct ttggaaaagg aaacttctca gagctgttca agtccataga agattacgag 1260
aaaagtctag aagccaagca gtctgctgca gtgcaaggtt cctga 1305
<210> 122
<211> 434
<212> PRT
<213> 人工序列-HvHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 122
Met Pro Pro Thr Pro Thr Thr Pro Ala Ala Thr Gly Ala Ala Ala Ala
1 5 10 15
Val Thr Pro Glu His Ala Arg Pro His Arg Met Val Arg Phe Asn Pro
20 25 30
Arg Ser Asp Arg Phe His Thr Leu Ser Phe His His Val Glu Phe Trp
35 40 45
Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ala Phe Ala Leu Gly
50 55 60
Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala His
65 70 75 80
Ala Ser Gln Leu Leu Arg Ser Gly Ser Leu Ala Phe Leu Phe Thr Ala
85 90 95
Pro Tyr Ala Asn Gly Cys Asp Ala Ala Thr Ala Ser Leu Pro Ser Phe
100 105 110
Ser Ala Asp Ala Ala Arg Arg Phe Ser Ala Asp His Gly Ile Ala Val
115 120 125
Arg Ser Val Ala Leu Arg Val Ala Asp Ala Ala Glu Ala Phe Arg Ala
130 135 140
Ser Arg Arg Arg Gly Ala Arg Pro Ala Phe Ala Pro Val Asp Leu Gly
145 150 155 160
Arg Gly Phe Ala Phe Ala Glu Val Glu Leu Tyr Gly Asp Val Val Leu
165 170 175
Arg Phe Val Ser His Pro Asp Gly Thr Asp Val Pro Phe Leu Pro Gly
180 185 190
Phe Glu Gly Val Thr Asn Pro Asp Ala Val Asp Tyr Gly Leu Thr Arg
195 200 205
Phe Asp His Val Val Gly Asn Val Pro Glu Leu Ala Pro Ala Ala Ala
210 215 220
Tyr Ile Ala Gly Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala
225 230 235 240
Glu Asp Val Gly Thr Thr Glu Ser Gly Leu Asn Ser Val Val Leu Ala
245 250 255
Asn Asn Ser Glu Gly Val Leu Leu Pro Leu Asn Glu Pro Val His Gly
260 265 270
Thr Lys Arg Arg Ser Gln Ile Gln Thr Phe Leu Glu His His Gly Gly
275 280 285
Pro Gly Val Gln His Ile Ala Val Ala Ser Ser Asp Val Leu Arg Thr
290 295 300
Leu Arg Lys Met Arg Ala Arg Ser Ala Met Gly Gly Phe Asp Phe Leu
305 310 315 320
Pro Pro Pro Leu Pro Lys Tyr Tyr Glu Gly Val Arg Arg Leu Ala Gly
325 330 335
Asp Val Leu Ser Glu Ala Gln Ile Lys Glu Cys Gln Glu Leu Gly Val
340 345 350
Leu Val Asp Arg Asp Asp Gln Gly Val Leu Leu Gln Ile Ala Thr Lys
355 360 365
Pro Val Gly Asp Arg Pro Thr Leu Phe Leu Glu Met Ile Gln Arg Ile
370 375 380
Gly Cys Met Glu Lys Asp Glu Arg Gly Glu Glu Tyr Gln Lys Gly Gly
385 390 395 400
Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile
405 410 415
Glu Asp Tyr Glu Lys Ser Leu Glu Ala Lys Gln Ser Ala Ala Val Gln
420 425 430
Gly Ser
<210> 123
<211> 1305
<212> DNA
<213> 人工序列-HvHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 123
atgccgccca cccccaccac ccccgcggct accggcgccg ccgccgcggt gacgccggag 60
cacgcgcgac cgcaccgaat ggtccgcttc aacccgcgca gcgaccgctt ccacacgctc 120
tccttccacc acgtcgagtt ctggtgcgcg gacgccgcct ccgccgccgg ccgcttcgcg 180
ttcgcgctcg gcgcgccgct cgccgccagg tccgacctct ccacggggaa ctccgcgcac 240
gcctcccagc tgctccgctc gggctccctc gccttcctct tcaccgcgcc ctacgccaac 300
ggctgcgacg ccgccaccgc ctccctgccc tccttctccg ccgacgccgc gcgccggttc 360
tccgccgacc acgggatcgc ggtgcgctcc gtagcgctgc gcgtcgcaga cgccgccgag 420
gccttccgcg ccagtcgtcg acggggcgcg cgcccggcct tcgcccccgt ggacctcggc 480
cgcggcttcg cgttcgcgga ggtcgagctc tacggcgacg tcgtgctccg cttcgtcagc 540
cacccggacg gcacggacgt gcccttcttg ccggggttcg agggcgtaac caacccggac 600
gccgtggact acggcctgac gcggttcgac cacgtcgtcg gcaacgtccc ggagcttgcc 660
cccgccgcag cctacatcgc cgggttcacg gggttccacg agttcgccga gttcacggcg 720
gaggacgtgg gcacgaccga gagcgggctc aactcggtgg tgctcgccaa caactcggag 780
ggcgtgctgc tgccgctcaa cgagccggtg cacggcacca agcgccggag ccagatacag 840
acgttcctgg aacaccacgg cggcccgggc gtgcagcaca tcgcggtggc cagcagtgac 900
gtgctcagga cgctcaggaa gatgcgtgcg cgctccgcca tgggcggctt cgacttcctg 960
ccacccccgc tgccgaagta ctacgaaggc gtgcgacgcc ttgccgggga tgtcctctcg 1020
gaggcgcaga tcaaggaatg ccaggagctg ggtgtgctcg tcgataggga cgaccaaggg 1080
gtgttgctcc aaatcgctac caagccagta ggggacaggc cgaccttgtt cctggagatg 1140
atccagagga tcgggtgcat ggagaaggac gagagagggg aagagtacca gaagggtggc 1200
tgcggcgggt tcggcaaagg caacttctcc gagctgttca agtccattga agattacgag 1260
aagtcccttg aagccaagca atctgctgca gttcagggat catag 1305
<210> 124
<211> 1305
<212> DNA
<213> 人工序列-HvHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 124
atgccgccga cgcccaccac cccagcagct accggagctg cggctgcagt aacccctgag 60
catgctcggc cccacaggat ggtgaggttt aatccaagat cagatcgttt tcatacactc 120
tcgttccacc atgttgagtt ctggtgcgcc gacgcggctt cagcggccgg gaggttcgcc 180
ttcgcgctgg gggcccctct tgccgcgcgt agtgatttgt ctactgggaa ttcagcgcac 240
gcgtcccagc ttctccgaag cggcagtctc gcctttctct tcactgcacc atatgcaaat 300
ggatgtgatg cagcaactgc ttccctgccg agcttttctg cggatgccgc gaggcgattc 360
agtgctgacc atgggatcgc cgtccggtca gttgctctta gagtggctga tgctgctgag 420
gcattcaggg cttcaaggcg tcgtggtgca cgcccggcgt ttgccccggt tgatcttggg 480
cgggggtttg cttttgcaga ggtggagctg tacggcgacg tggtgctccg atttgtttct 540
cacccggacg gcactgatgt gcccttcttg ccaggatttg aaggtgtgac caaccctgat 600
gccgtggatt atggactcac aagatttgat catgtggttg gcaatgttcc tgaattggca 660
cctgctgccg cttacatcgc aggttttact ggcttccatg aatttgctga attcacggcg 720
gaggatgttg gtacaactga gtcagggctg aacagtgttg ttttagccaa caactcagaa 780
ggagttctcc ttcccttgaa tgaacctgtt catggaacca agcgccgctc ccaaattcaa 840
acatttcttg agcaccacgg cggccctggt gttcaacata ttgctgtcgc ctctagcgac 900
gtcctgcgta cattacgcaa gatgagggct cggtccgcaa tgggtggttt cgacttcctc 960
cctccaccat tgccaaaata ttatgaaggc gtcagaagac ttgcaggaga tgtcctctct 1020
gaagctcaga tcaaggagtg ccaagaactt ggcgtcttgg ttgatagaga tgaccaagga 1080
gtacttctgc agatagccac aaaacccgtt ggtgacaggc caactctttt cctggagatg 1140
attcagagaa ttggctgcat ggagaaagat gaaagaggcg aggagtacca gaagggtggt 1200
tgtggaggct ttggaaaagg aaacttctca gagctgttca agtccataga agattacgag 1260
aaaagtctag aagccaagca gtctgctgca gtgcaaggtt cctga 1305
<210> 125
<211> 434
<212> PRT
<213> 人工序列-HvHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 125
Met Pro Pro Thr Pro Thr Thr Pro Ala Ala Thr Gly Ala Ala Ala Ala
1 5 10 15
Val Thr Pro Glu His Ala Arg Pro His Arg Met Val Arg Phe Asn Pro
20 25 30
Arg Ser Asp Arg Phe His Thr Leu Ser Phe His His Val Glu Phe Trp
35 40 45
Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ala Phe Ala Leu Gly
50 55 60
Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala His
65 70 75 80
Ala Ser Gln Leu Leu Arg Ser Gly Ser Leu Ala Phe Leu Phe Thr Ala
85 90 95
Pro Tyr Ala Asn Gly Cys Asp Ala Ala Thr Ala Ser Leu Pro Ser Phe
100 105 110
Ser Ala Asp Ala Ala Arg Arg Phe Ser Ala Asp His Gly Ile Ala Val
115 120 125
Arg Ser Val Ala Leu Arg Val Ala Asp Ala Ala Glu Ala Phe Arg Ala
130 135 140
Ser Arg Arg Arg Gly Ala Arg Pro Ala Phe Ala Pro Val Asp Leu Gly
145 150 155 160
Arg Gly Phe Ala Phe Ala Glu Val Glu Leu Tyr Gly Asp Val Val Leu
165 170 175
Arg Phe Val Ser His Pro Asp Gly Thr Asp Val Pro Phe Leu Pro Gly
180 185 190
Phe Glu Gly Val Thr Asn Pro Asp Ala Val Asp Tyr Gly Leu Thr Arg
195 200 205
Phe Asp His Val Val Gly Asn Val Pro Glu Leu Ala Pro Ala Ala Ala
210 215 220
Tyr Ile Ala Gly Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala
225 230 235 240
Glu Asp Val Gly Thr Thr Glu Ser Gly Leu Asn Ser Val Val Leu Ala
245 250 255
Asn Asn Ser Glu Gly Val Leu Leu Pro Leu Asn Glu Pro Val His Gly
260 265 270
Thr Lys Arg Arg Ser Gln Ile Gln Thr Phe Leu Glu His His Gly Gly
275 280 285
Pro Gly Val Gln His Ile Ala Val Ala Ser Ser Asp Val Leu Arg Thr
290 295 300
Leu Arg Lys Met Arg Ala Arg Ser Ala Met Gly Gly Phe Asp Phe Leu
305 310 315 320
Pro Pro Pro Leu Pro Lys Tyr Tyr Glu Gly Val Arg Arg Leu Ala Gly
325 330 335
Asp Val Leu Ser Glu Ala Gln Ile Lys Glu Cys Gln Glu Leu Gly Val
340 345 350
Leu Val Asp Arg Asp Asp Gln Gly Val Leu Leu Gln Ile Phe Thr Lys
355 360 365
Pro Val Gly Asp Arg Pro Thr Leu Trp Leu Glu Met Ile Gln Arg Ile
370 375 380
Gly Cys Met Glu Lys Asp Glu Arg Gly Glu Glu Tyr Gln Lys Gly Gly
385 390 395 400
Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile
405 410 415
Glu Asp Tyr Glu Lys Ser Leu Glu Ala Lys Gln Ser Ala Ala Val Gln
420 425 430
Gly Ser
<210> 126
<211> 1305
<212> DNA
<213> 人工序列-HvHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 126
atgccgccca cccccaccac ccccgcggct accggcgccg ccgccgcggt gacgccggag 60
cacgcgcgac cgcaccgaat ggtccgcttc aacccgcgca gcgaccgctt ccacacgctc 120
tccttccacc acgtcgagtt ctggtgcgcg gacgccgcct ccgccgccgg ccgcttcgcg 180
ttcgcgctcg gcgcgccgct cgccgccagg tccgacctct ccacggggaa ctccgcgcac 240
gcctcccagc tgctccgctc gggctccctc gccttcctct tcaccgcgcc ctacgccaac 300
ggctgcgacg ccgccaccgc ctccctgccc tccttctccg ccgacgccgc gcgccggttc 360
tccgccgacc acgggatcgc ggtgcgctcc gtagcgctgc gcgtcgcaga cgccgccgag 420
gccttccgcg ccagtcgtcg acggggcgcg cgcccggcct tcgcccccgt ggacctcggc 480
cgcggcttcg cgttcgcgga ggtcgagctc tacggcgacg tcgtgctccg cttcgtcagc 540
cacccggacg gcacggacgt gcccttcttg ccggggttcg agggcgtaac caacccggac 600
gccgtggact acggcctgac gcggttcgac cacgtcgtcg gcaacgtccc ggagcttgcc 660
cccgccgcag cctacatcgc cgggttcacg gggttccacg agttcgccga gttcacggcg 720
gaggacgtgg gcacgaccga gagcgggctc aactcggtgg tgctcgccaa caactcggag 780
ggcgtgctgc tgccgctcaa cgagccggtg cacggcacca agcgccggag ccagatacag 840
acgttcctgg aacaccacgg cggcccgggc gtgcagcaca tcgcggtggc cagcagtgac 900
gtgctcagga cgctcaggaa gatgcgtgcg cgctccgcca tgggcggctt cgacttcctg 960
ccacccccgc tgccgaagta ctacgaaggc gtgcgacgcc ttgccgggga tgtcctctcg 1020
gaggcgcaga tcaaggaatg ccaggagctg ggtgtgctcg tcgataggga cgaccaaggg 1080
gtgttgctcc aaatcttcac caagccagta ggggacaggc cgaccttgtg gctggagatg 1140
atccagagga tcgggtgcat ggagaaggac gagagagggg aagagtacca gaagggtggc 1200
tgcggcgggt tcggcaaagg caacttctcc gagctgttca agtccattga agattacgag 1260
aagtcccttg aagccaagca atctgctgca gttcagggat catag 1305
<210> 127
<211> 1305
<212> DNA
<213> 人工序列-HvHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 127
atgccgccga cgcccaccac cccagcagct accggagctg cggctgcagt aacccctgag 60
catgctcggc cccacaggat ggtgaggttt aatccaagat cagatcgttt tcatacactc 120
tcgttccacc atgttgagtt ctggtgcgcc gacgcggctt cagcggccgg gaggttcgcc 180
ttcgcgctgg gggcccctct tgccgcgcgt agtgatttgt ctactgggaa ttcagcgcac 240
gcgtcccagc ttctccgaag cggcagtctc gcctttctct tcactgcacc atatgcaaat 300
ggatgtgatg cagcaactgc ttccctgccg agcttttctg cggatgccgc gaggcgattc 360
agtgctgacc atgggatcgc cgtccggtca gttgctctta gagtggctga tgctgctgag 420
gcattcaggg cttcaaggcg tcgtggtgca cgcccggcgt ttgccccggt tgatcttggg 480
cgggggtttg cttttgcaga ggtggagctg tacggcgacg tggtgctccg atttgtttct 540
cacccggacg gcactgatgt gcccttcttg ccaggatttg aaggtgtgac caaccctgat 600
gccgtggatt atggactcac aagatttgat catgtggttg gcaatgttcc tgaattggca 660
cctgctgccg cttacatcgc aggttttact ggcttccatg aatttgctga attcacggcg 720
gaggatgttg gtacaactga gtcagggctg aacagtgttg ttttagccaa caactcagaa 780
ggagttctcc ttcccttgaa tgaacctgtt catggaacca agcgccgctc ccaaattcaa 840
acatttcttg agcaccacgg cggccctggt gttcaacata ttgctgtcgc ctctagcgac 900
gtcctgcgta cattacgcaa gatgagggct cggtccgcaa tgggtggttt cgacttcctc 960
cctccaccat tgccaaaata ttatgaaggc gtcagaagac ttgcaggaga tgtcctctct 1020
gaagctcaga tcaaggagtg ccaagaactt ggcgtcttgg ttgatagaga tgaccaagga 1080
gtacttctgc agatatttac aaaacccgtt ggtgacaggc caactctttg gctggagatg 1140
attcagagaa ttggctgcat ggagaaagat gaaagaggcg aggagtacca gaagggtggt 1200
tgtggaggct ttggaaaagg aaacttctca gagctgttca agtccataga agattacgag 1260
aaaagtctag aagccaagca gtctgctgca gtgcaaggtt cctga 1305
<210> 128
<211> 434
<212> PRT
<213> 人工序列-HvHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 128
Met Pro Pro Thr Pro Thr Thr Pro Ala Ala Thr Gly Ala Ala Ala Ala
1 5 10 15
Val Thr Pro Glu His Ala Arg Pro His Arg Met Val Arg Phe Asn Pro
20 25 30
Arg Ser Asp Arg Phe His Thr Leu Ser Phe His His Val Glu Phe Trp
35 40 45
Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ala Phe Ala Leu Gly
50 55 60
Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala His
65 70 75 80
Ala Ser Gln Leu Leu Arg Ser Gly Ser Leu Ala Phe Leu Phe Thr Ala
85 90 95
Pro Tyr Ala Asn Gly Cys Asp Ala Ala Thr Ala Ser Leu Pro Ser Phe
100 105 110
Ser Ala Asp Ala Ala Arg Arg Phe Ser Ala Asp His Gly Ile Ala Val
115 120 125
Arg Ser Val Ala Leu Arg Val Ala Asp Ala Ala Glu Ala Phe Arg Ala
130 135 140
Ser Arg Arg Arg Gly Ala Arg Pro Ala Phe Ala Pro Val Asp Leu Gly
145 150 155 160
Arg Gly Phe Ala Phe Ala Glu Val Glu Leu Tyr Gly Asp Val Val Leu
165 170 175
Arg Phe Val Ser His Pro Asp Gly Thr Asp Val Pro Phe Leu Pro Gly
180 185 190
Phe Glu Gly Val Thr Asn Pro Asp Ala Val Asp Tyr Gly Leu Thr Arg
195 200 205
Phe Asp His Val Val Gly Asn Val Pro Glu Leu Ala Pro Ala Ala Ala
210 215 220
Tyr Ile Ala Gly Phe Thr Gly Phe His Glu Phe Ala Glu Phe Thr Ala
225 230 235 240
Glu Asp Val Gly Thr Thr Glu Ser Gly Leu Asn Ser Val Val Leu Ala
245 250 255
Asn Asn Ser Glu Gly Val Leu Leu Pro Leu Asn Glu Pro Val His Gly
260 265 270
Thr Lys Arg Arg Ser Gln Ile Gln Thr Phe Leu Glu His His Gly Gly
275 280 285
Pro Gly Val Gln His Ile Ala Val Ala Ser Ser Asp Val Leu Arg Thr
290 295 300
Leu Arg Lys Met Arg Ala Arg Ser Ala Met Gly Gly Phe Asp Phe Leu
305 310 315 320
Pro Pro Pro Leu Pro Lys Tyr Tyr Glu Gly Val Arg Arg Leu Ala Gly
325 330 335
Asp Val Leu Ser Glu Ala Gln Ile Lys Glu Cys Gln Glu Leu Gly Val
340 345 350
Leu Val Asp Arg Asp Asp Gln Gly Val Leu Leu Gln Ile Ala Thr Lys
355 360 365
Pro Val Gly Asp Arg Pro Thr Leu Trp Leu Glu Met Ile Gln Arg Ile
370 375 380
Gly Cys Met Glu Lys Asp Glu Arg Gly Glu Glu Tyr Gln Lys Gly Gly
385 390 395 400
Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile
405 410 415
Glu Asp Tyr Glu Lys Ser Leu Glu Ala Lys Gln Ser Ala Ala Val Gln
420 425 430
Gly Ser
<210> 129
<211> 1305
<212> DNA
<213> 人工序列-HvHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 129
atgccgccca cccccaccac ccccgcggct accggcgccg ccgccgcggt gacgccggag 60
cacgcgcgac cgcaccgaat ggtccgcttc aacccgcgca gcgaccgctt ccacacgctc 120
tccttccacc acgtcgagtt ctggtgcgcg gacgccgcct ccgccgccgg ccgcttcgcg 180
ttcgcgctcg gcgcgccgct cgccgccagg tccgacctct ccacggggaa ctccgcgcac 240
gcctcccagc tgctccgctc gggctccctc gccttcctct tcaccgcgcc ctacgccaac 300
ggctgcgacg ccgccaccgc ctccctgccc tccttctccg ccgacgccgc gcgccggttc 360
tccgccgacc acgggatcgc ggtgcgctcc gtagcgctgc gcgtcgcaga cgccgccgag 420
gccttccgcg ccagtcgtcg acggggcgcg cgcccggcct tcgcccccgt ggacctcggc 480
cgcggcttcg cgttcgcgga ggtcgagctc tacggcgacg tcgtgctccg cttcgtcagc 540
cacccggacg gcacggacgt gcccttcttg ccggggttcg agggcgtaac caacccggac 600
gccgtggact acggcctgac gcggttcgac cacgtcgtcg gcaacgtccc ggagcttgcc 660
cccgccgcag cctacatcgc cgggttcacg gggttccacg agttcgccga gttcacggcg 720
gaggacgtgg gcacgaccga gagcgggctc aactcggtgg tgctcgccaa caactcggag 780
ggcgtgctgc tgccgctcaa cgagccggtg cacggcacca agcgccggag ccagatacag 840
acgttcctgg aacaccacgg cggcccgggc gtgcagcaca tcgcggtggc cagcagtgac 900
gtgctcagga cgctcaggaa gatgcgtgcg cgctccgcca tgggcggctt cgacttcctg 960
ccacccccgc tgccgaagta ctacgaaggc gtgcgacgcc ttgccgggga tgtcctctcg 1020
gaggcgcaga tcaaggaatg ccaggagctg ggtgtgctcg tcgataggga cgaccaaggg 1080
gtgttgctcc aaatcgctac caagccagta ggggacaggc cgaccttgtg gctggagatg 1140
atccagagga tcgggtgcat ggagaaggac gagagagggg aagagtacca gaagggtggc 1200
tgcggcgggt tcggcaaagg caacttctcc gagctgttca agtccattga agattacgag 1260
aagtcccttg aagccaagca atctgctgca gttcagggat catag 1305
<210> 130
<211> 1305
<212> DNA
<213> 人工序列-HvHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 130
atgccgccga cgcccaccac cccagcagct accggagctg cggctgcagt aacccctgag 60
catgctcggc cccacaggat ggtgaggttt aatccaagat cagatcgttt tcatacactc 120
tcgttccacc atgttgagtt ctggtgcgcc gacgcggctt cagcggccgg gaggttcgcc 180
ttcgcgctgg gggcccctct tgccgcgcgt agtgatttgt ctactgggaa ttcagcgcac 240
gcgtcccagc ttctccgaag cggcagtctc gcctttctct tcactgcacc atatgcaaat 300
ggatgtgatg cagcaactgc ttccctgccg agcttttctg cggatgccgc gaggcgattc 360
agtgctgacc atgggatcgc cgtccggtca gttgctctta gagtggctga tgctgctgag 420
gcattcaggg cttcaaggcg tcgtggtgca cgcccggcgt ttgccccggt tgatcttggg 480
cgggggtttg cttttgcaga ggtggagctg tacggcgacg tggtgctccg atttgtttct 540
cacccggacg gcactgatgt gcccttcttg ccaggatttg aaggtgtgac caaccctgat 600
gccgtggatt atggactcac aagatttgat catgtggttg gcaatgttcc tgaattggca 660
cctgctgccg cttacatcgc aggttttact ggcttccatg aatttgctga attcacggcg 720
gaggatgttg gtacaactga gtcagggctg aacagtgttg ttttagccaa caactcagaa 780
ggagttctcc ttcccttgaa tgaacctgtt catggaacca agcgccgctc ccaaattcaa 840
acatttcttg agcaccacgg cggccctggt gttcaacata ttgctgtcgc ctctagcgac 900
gtcctgcgta cattacgcaa gatgagggct cggtccgcaa tgggtggttt cgacttcctc 960
cctccaccat tgccaaaata ttatgaaggc gtcagaagac ttgcaggaga tgtcctctct 1020
gaagctcaga tcaaggagtg ccaagaactt ggcgtcttgg ttgatagaga tgaccaagga 1080
gtacttctgc agatagccac aaaacccgtt ggtgacaggc caactctttg gctggagatg 1140
attcagagaa ttggctgcat ggagaaagat gaaagaggcg aggagtacca gaagggtggt 1200
tgtggaggct ttggaaaagg aaacttctca gagctgttca agtccataga agattacgag 1260
aaaagtctag aagccaagca gtctgctgca gtgcaaggtt cctga 1305
<210> 131
<211> 444
<212> PRT
<213> 玉米野生型HPPD(ZmHPPD的氨基酸序列Zea mays)
<400> 131
Met Pro Pro Thr Pro Thr Ala Ala Ala Ala Gly Ala Ala Val Ala Ala
1 5 10 15
Ala Ser Ala Ala Glu Gln Ala Ala Phe Arg Leu Val Gly His Arg Asn
20 25 30
Phe Val Arg Phe Asn Pro Arg Ser Asp Arg Phe His Thr Leu Ala Phe
35 40 45
His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg
50 55 60
Phe Ser Phe Gly Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Ser Ala His Ala Ser Leu Leu Leu Arg Ser Gly Ser Leu
85 90 95
Ser Phe Leu Phe Thr Ala Pro Tyr Ala His Gly Ala Asp Ala Ala Thr
100 105 110
Ala Ala Leu Pro Ser Phe Ser Ala Ala Ala Ala Arg Arg Phe Ala Ala
115 120 125
Asp His Gly Leu Ala Val Arg Ala Val Ala Leu Arg Val Ala Asp Ala
130 135 140
Glu Glu Ala Phe Arg Thr Ser Val Ala Ala Gly Ala Arg Pro Ala Phe
145 150 155 160
Gly Pro Val Asp Leu Gly Arg Gly Phe Arg Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Pro Asp Gly Ala Ala
180 185 190
Gly Glu Pro Phe Leu Pro Gly Phe Glu Gly Val Ala Ser Pro Gly Ala
195 200 205
Ala Asp Tyr Gly Leu Ser Arg Phe Asp His Ile Val Gly Asn Val Pro
210 215 220
Glu Leu Ala Pro Ala Ala Ala Tyr Phe Ala Gly Phe Thr Gly Phe His
225 230 235 240
Glu Phe Ala Glu Phe Thr Thr Glu Asp Val Gly Thr Ala Glu Ser Gly
245 250 255
Leu Asn Ser Met Val Leu Ala Asn Asn Ser Glu Asn Val Leu Leu Pro
260 265 270
Leu Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr
275 280 285
Phe Leu Asp His His Gly Gly Pro Gly Val Gln His Met Ala Leu Ala
290 295 300
Ser Asp Asp Val Leu Arg Thr Leu Arg Glu Met Gln Ala Arg Ser Ala
305 310 315 320
Met Gly Gly Phe Glu Phe Met Ala Pro Pro Thr Ser Asp Tyr Tyr Asp
325 330 335
Gly Val Arg Arg Arg Ala Gly Asp Val Leu Thr Glu Ala Gln Ile Lys
340 345 350
Glu Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val
355 360 365
Leu Leu Gln Ile Phe Pro Lys Pro Val Gly Asp Arg Pro Thr Leu Phe
370 375 380
Leu Glu Ile Ile Gln Arg Ile Gly Cys Met Glu Arg Asp Glu Lys Gly
385 390 395 400
Gln Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe
405 410 415
Ser Gln Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Ala
420 425 430
Met Gln Ala Ala Ala Ala Ala Thr Ala Gln Gly Ser
435 440
<210> 132
<211> 1335
<212> DNA
<213> ZmHPPD-01核苷酸序列(Zea mays)
<400> 132
atgcccccga cccccacagc cgccgcagcc ggcgccgccg tggcggcggc atcagcagcg 60
gagcaggcgg cgttccgcct cgtgggccac cgcaacttcg tccgcttcaa cccgcgctcc 120
gaccgcttcc acacgctcgc gttccaccac gtggagctct ggtgcgccga cgcggcctcc 180
gccgcgggcc gcttctcctt cggcctgggc gcgccgctcg ccgcgcgctc cgacctctcc 240
acgggcaact ccgcgcacgc gtccctgctg ctccgctccg gctccctctc cttcctcttc 300
acggcgccct acgcgcacgg cgccgacgcc gccaccgctg cgctgccctc cttctccgcc 360
gccgccgcgc ggcgcttcgc ggccgaccac ggcctcgcgg tgcgcgccgt cgcgctccgc 420
gtcgccgacg ccgaggaggc cttccgcacc agcgtcgcgg ccggggcgcg cccggctttc 480
ggccccgtcg acctcggccg cggcttccgc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctccggt acgtgagcta cccggacggc gcggcgggcg agcccttcct gccggggttc 600
gagggcgtgg ccagccccgg ggcggccgac tacgggctga gcaggttcga ccacatcgtc 660
ggcaacgtgc cggagctggc gcccgccgcc gcctacttcg ccggcttcac ggggttccac 720
gagttcgccg agttcacgac ggaggacgtg ggcaccgcgg agagcggcct caactccatg 780
gtgctcgcca acaactcgga gaacgtgctg ctcccgctca acgagccggt gcacggcacc 840
aagcgccgca gccagataca gacgttcctg gaccaccacg gcggccccgg cgtgcagcac 900
atggcgctgg ccagcgacga cgtgctcagg acgctgaggg agatgcaggc gcgctcggcc 960
atgggcggct tcgagttcat ggcgcctccc acatccgact actatgacgg cgtgaggcgg 1020
cgcgccgggg acgtgctcac ggaagcacag attaaggagt gccaggagct aggggtgctg 1080
gtggataggg atgaccaggg cgtgctgctc caaatcttcc ccaagccagt gggggacagg 1140
ccaacgctgt tcttggagat catccaaagg atcgggtgca tggagaggga tgagaagggg 1200
caagaatacc agaagggtgg ctgcggcggg ttcggcaagg gaaacttctc gcagctgttc 1260
aaatccatcg aggattatga gaagtccctt gaagccatgc aagctgctgc agcagctaca 1320
gctcagggat cctag 1335
<210> 133
<211> 1335
<212> DNA
<213> 人工序列-ZmHPPD-02核苷酸序列(Artificial Sequence)
<400> 133
atgccaccca cacccacagc agcagcggcg ggcgcagcgg tcgcagcggc gtcagcggcg 60
gagcaagcgg cgttcagatt ggttggtcac cgcaacttcg tgagattcaa tcctcgctca 120
gatagattcc atactctggc cttccaccat gttgagcttt ggtgcgctga tgctgcttct 180
gctgctggaa ggttcagctt cggtttgggt gctcccctcg ctgctagatc tgatttgagc 240
actggtaact cagcacatgc ttccctcctg cttagatctg gctctctgag cttccttttc 300
actgctccat atgctcatgg tgctgatgct gctacagctg ctctcccttc attctccgct 360
gctgctgcta ggagattcgc tgctgatcac ggattggctg tgagggctgt tgcacttaga 420
gttgctgatg ctgaggaggc cttccgcacc tctgttgctg ctggagccag accagctttc 480
ggacctgttg atctcggaag gggtttccgc ctggctgagg tggagcttta cggcgatgtg 540
gttctgcgct acgttagcta tcctgatggt gctgctggtg aacctttcct tccaggattc 600
gagggtgtgg cctcaccagg tgccgctgat tacggcctgt ccagattcga ccacattgtt 660
ggaaatgttc ctgagcttgc tccagctgct gcctacttcg ctggcttcac cggattccat 720
gagttcgccg agttcaccac tgaggatgtg ggaactgctg agtctggttt gaatagcatg 780
gtgctcgcca acaattcaga gaacgttttg ctccctctga atgagcccgt gcacggaaca 840
aagagaaggt cccagattca aaccttcttg gatcaccacg gcggcccagg tgttcagcat 900
atggctctcg cctccgatga cgtgttgaga accctcaggg agatgcaagc caggtctgct 960
atgggtggct tcgagttcat ggccccacct acaagcgatt actatgatgg tgtgcgcaga 1020
agggctggcg acgttcttac cgaggctcag atcaaggagt gccaagagct gggcgtgctt 1080
gttgatagag atgaccaggg agttctgctt caaattttcc ccaagccagt gggcgacagg 1140
cctactttgt tcctcgagat tatccagaga atcggatgca tggagaggga tgagaagggt 1200
caggagtacc aaaagggagg ttgcggcgga ttcggcaagg gaaatttctc acaactcttc 1260
aagtccatcg aggactatga aaagagtctg gaagcgatgc aggcagcagc agcagcaacc 1320
gcacaggggt cgtga 1335
<210> 134
<211> 444
<212> PRT
<213> 人工序列-ZmHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 134
Met Pro Pro Thr Pro Thr Ala Ala Ala Ala Gly Ala Ala Val Ala Ala
1 5 10 15
Ala Ser Ala Ala Glu Gln Ala Ala Phe Arg Leu Val Gly His Arg Asn
20 25 30
Phe Val Arg Phe Asn Pro Arg Ser Asp Arg Phe His Thr Leu Ala Phe
35 40 45
His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg
50 55 60
Phe Ser Phe Gly Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Ser Ala His Ala Ser Leu Leu Leu Arg Ser Gly Ser Leu
85 90 95
Ser Phe Leu Phe Thr Ala Pro Tyr Ala His Gly Ala Asp Ala Ala Thr
100 105 110
Ala Ala Leu Pro Ser Phe Ser Ala Ala Ala Ala Arg Arg Phe Ala Ala
115 120 125
Asp His Gly Leu Ala Val Arg Ala Val Ala Leu Arg Val Ala Asp Ala
130 135 140
Glu Glu Ala Phe Arg Thr Ser Val Ala Ala Gly Ala Arg Pro Ala Phe
145 150 155 160
Gly Pro Val Asp Leu Gly Arg Gly Phe Arg Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Pro Asp Gly Ala Ala
180 185 190
Gly Glu Pro Phe Leu Pro Gly Phe Glu Gly Val Ala Ser Pro Gly Ala
195 200 205
Ala Asp Tyr Gly Leu Ser Arg Phe Asp His Ile Val Gly Asn Val Pro
210 215 220
Glu Leu Ala Pro Ala Ala Ala Tyr Phe Ala Gly Phe Thr Gly Phe His
225 230 235 240
Glu Phe Ala Glu Phe Thr Thr Glu Asp Val Gly Thr Ala Glu Ser Gly
245 250 255
Leu Asn Ser Met Val Leu Ala Asn Asn Ser Glu Asn Val Leu Leu Pro
260 265 270
Leu Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr
275 280 285
Phe Leu Asp His His Gly Gly Pro Gly Val Gln His Met Ala Leu Ala
290 295 300
Ser Asp Asp Val Leu Arg Thr Leu Arg Glu Met Gln Ala Arg Ser Ala
305 310 315 320
Met Gly Gly Phe Glu Phe Met Ala Pro Pro Thr Ser Asp Tyr Tyr Asp
325 330 335
Gly Val Arg Arg Arg Ala Gly Asp Val Leu Thr Glu Ala Gln Ile Lys
340 345 350
Glu Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val
355 360 365
Leu Leu Gln Ile Ala Pro Lys Pro Val Gly Asp Arg Pro Thr Leu Phe
370 375 380
Leu Glu Ile Ile Gln Arg Ile Gly Cys Met Glu Arg Asp Glu Lys Gly
385 390 395 400
Gln Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe
405 410 415
Ser Gln Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Ala
420 425 430
Met Gln Ala Ala Ala Ala Ala Thr Ala Gln Gly Ser
435 440
<210> 135
<211> 1335
<212> DNA
<213> 人工序列-ZmHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 135
atgcccccga cccccacagc cgccgcagcc ggcgccgccg tggcggcggc atcagcagcg 60
gagcaggcgg cgttccgcct cgtgggccac cgcaacttcg tccgcttcaa cccgcgctcc 120
gaccgcttcc acacgctcgc gttccaccac gtggagctct ggtgcgccga cgcggcctcc 180
gccgcgggcc gcttctcctt cggcctgggc gcgccgctcg ccgcgcgctc cgacctctcc 240
acgggcaact ccgcgcacgc gtccctgctg ctccgctccg gctccctctc cttcctcttc 300
acggcgccct acgcgcacgg cgccgacgcc gccaccgctg cgctgccctc cttctccgcc 360
gccgccgcgc ggcgcttcgc ggccgaccac ggcctcgcgg tgcgcgccgt cgcgctccgc 420
gtcgccgacg ccgaggaggc cttccgcacc agcgtcgcgg ccggggcgcg cccggctttc 480
ggccccgtcg acctcggccg cggcttccgc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctccggt acgtgagcta cccggacggc gcggcgggcg agcccttcct gccggggttc 600
gagggcgtgg ccagccccgg ggcggccgac tacgggctga gcaggttcga ccacatcgtc 660
ggcaacgtgc cggagctggc gcccgccgcc gcctacttcg ccggcttcac ggggttccac 720
gagttcgccg agttcacgac ggaggacgtg ggcaccgcgg agagcggcct caactccatg 780
gtgctcgcca acaactcgga gaacgtgctg ctcccgctca acgagccggt gcacggcacc 840
aagcgccgca gccagataca gacgttcctg gaccaccacg gcggccccgg cgtgcagcac 900
atggcgctgg ccagcgacga cgtgctcagg acgctgaggg agatgcaggc gcgctcggcc 960
atgggcggct tcgagttcat ggcgcctccc acatccgact actatgacgg cgtgaggcgg 1020
cgcgccgggg acgtgctcac ggaagcacag attaaggagt gccaggagct aggggtgctg 1080
gtggataggg atgaccaggg cgtgctgctc caaatcgctc ccaagccagt gggggacagg 1140
ccaacgctgt tcttggagat catccaaagg atcgggtgca tggagaggga tgagaagggg 1200
caagaatacc agaagggtgg ctgcggcggg ttcggcaagg gaaacttctc gcagctgttc 1260
aaatccatcg aggattatga gaagtccctt gaagccatgc aagctgctgc agcagctaca 1320
gctcagggat cctag 1335
<210> 136
<211> 1335
<212> DNA
<213> 人工序列-ZmHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 136
atgccaccca cacccacagc agcagcggcg ggcgcagcgg tcgcagcggc gtcagcggcg 60
gagcaagcgg cgttcagatt ggttggtcac cgcaacttcg tgagattcaa tcctcgctca 120
gatagattcc atactctggc cttccaccat gttgagcttt ggtgcgctga tgctgcttct 180
gctgctggaa ggttcagctt cggtttgggt gctcccctcg ctgctagatc tgatttgagc 240
actggtaact cagcacatgc ttccctcctg cttagatctg gctctctgag cttccttttc 300
actgctccat atgctcatgg tgctgatgct gctacagctg ctctcccttc attctccgct 360
gctgctgcta ggagattcgc tgctgatcac ggattggctg tgagggctgt tgcacttaga 420
gttgctgatg ctgaggaggc cttccgcacc tctgttgctg ctggagccag accagctttc 480
ggacctgttg atctcggaag gggtttccgc ctggctgagg tggagcttta cggcgatgtg 540
gttctgcgct acgttagcta tcctgatggt gctgctggtg aacctttcct tccaggattc 600
gagggtgtgg cctcaccagg tgccgctgat tacggcctgt ccagattcga ccacattgtt 660
ggaaatgttc ctgagcttgc tccagctgct gcctacttcg ctggcttcac cggattccat 720
gagttcgccg agttcaccac tgaggatgtg ggaactgctg agtctggttt gaatagcatg 780
gtgctcgcca acaattcaga gaacgttttg ctccctctga atgagcccgt gcacggaaca 840
aagagaaggt cccagattca aaccttcttg gatcaccacg gcggcccagg tgttcagcat 900
atggctctcg cctccgatga cgtgttgaga accctcaggg agatgcaagc caggtctgct 960
atgggtggct tcgagttcat ggccccacct acaagcgatt actatgatgg tgtgcgcaga 1020
agggctggcg acgttcttac cgaggctcag atcaaggagt gccaagagct gggcgtgctt 1080
gttgatagag atgaccaggg agttctgctt caaattgctc ccaagccagt gggcgacagg 1140
cctactttgt tcctcgagat tatccagaga atcggatgca tggagaggga tgagaagggt 1200
caggagtacc aaaagggagg ttgcggcgga ttcggcaagg gaaatttctc acaactcttc 1260
aagtccatcg aggactatga aaagagtctg gaagcgatgc aggcagcagc agcagcaacc 1320
gcacaggggt cgtga 1335
<210> 137
<211> 444
<212> PRT
<213> 人工序列-ZmHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 137
Met Pro Pro Thr Pro Thr Ala Ala Ala Ala Gly Ala Ala Val Ala Ala
1 5 10 15
Ala Ser Ala Ala Glu Gln Ala Ala Phe Arg Leu Val Gly His Arg Asn
20 25 30
Phe Val Arg Phe Asn Pro Arg Ser Asp Arg Phe His Thr Leu Ala Phe
35 40 45
His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg
50 55 60
Phe Ser Phe Gly Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Ser Ala His Ala Ser Leu Leu Leu Arg Ser Gly Ser Leu
85 90 95
Ser Phe Leu Phe Thr Ala Pro Tyr Ala His Gly Ala Asp Ala Ala Thr
100 105 110
Ala Ala Leu Pro Ser Phe Ser Ala Ala Ala Ala Arg Arg Phe Ala Ala
115 120 125
Asp His Gly Leu Ala Val Arg Ala Val Ala Leu Arg Val Ala Asp Ala
130 135 140
Glu Glu Ala Phe Arg Thr Ser Val Ala Ala Gly Ala Arg Pro Ala Phe
145 150 155 160
Gly Pro Val Asp Leu Gly Arg Gly Phe Arg Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Pro Asp Gly Ala Ala
180 185 190
Gly Glu Pro Phe Leu Pro Gly Phe Glu Gly Val Ala Ser Pro Gly Ala
195 200 205
Ala Asp Tyr Gly Leu Ser Arg Phe Asp His Ile Val Gly Asn Val Pro
210 215 220
Glu Leu Ala Pro Ala Ala Ala Tyr Phe Ala Gly Phe Thr Gly Phe His
225 230 235 240
Glu Phe Ala Glu Phe Thr Thr Glu Asp Val Gly Thr Ala Glu Ser Gly
245 250 255
Leu Asn Ser Met Val Leu Ala Asn Asn Ser Glu Asn Val Leu Leu Pro
260 265 270
Leu Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr
275 280 285
Phe Leu Asp His His Gly Gly Pro Gly Val Gln His Met Ala Leu Ala
290 295 300
Ser Asp Asp Val Leu Arg Thr Leu Arg Glu Met Gln Ala Arg Ser Ala
305 310 315 320
Met Gly Gly Phe Glu Phe Met Ala Pro Pro Thr Ser Asp Tyr Tyr Asp
325 330 335
Gly Val Arg Arg Arg Ala Gly Asp Val Leu Thr Glu Ala Gln Ile Lys
340 345 350
Glu Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val
355 360 365
Leu Leu Gln Ile Phe Pro Lys Pro Val Gly Asp Arg Pro Thr Leu Trp
370 375 380
Leu Glu Ile Ile Gln Arg Ile Gly Cys Met Glu Arg Asp Glu Lys Gly
385 390 395 400
Gln Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe
405 410 415
Ser Gln Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Ala
420 425 430
Met Gln Ala Ala Ala Ala Ala Thr Ala Gln Gly Ser
435 440
<210> 138
<211> 1335
<212> DNA
<213> 人工序列-ZmHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 138
atgcccccga cccccacagc cgccgcagcc ggcgccgccg tggcggcggc atcagcagcg 60
gagcaggcgg cgttccgcct cgtgggccac cgcaacttcg tccgcttcaa cccgcgctcc 120
gaccgcttcc acacgctcgc gttccaccac gtggagctct ggtgcgccga cgcggcctcc 180
gccgcgggcc gcttctcctt cggcctgggc gcgccgctcg ccgcgcgctc cgacctctcc 240
acgggcaact ccgcgcacgc gtccctgctg ctccgctccg gctccctctc cttcctcttc 300
acggcgccct acgcgcacgg cgccgacgcc gccaccgctg cgctgccctc cttctccgcc 360
gccgccgcgc ggcgcttcgc ggccgaccac ggcctcgcgg tgcgcgccgt cgcgctccgc 420
gtcgccgacg ccgaggaggc cttccgcacc agcgtcgcgg ccggggcgcg cccggctttc 480
ggccccgtcg acctcggccg cggcttccgc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctccggt acgtgagcta cccggacggc gcggcgggcg agcccttcct gccggggttc 600
gagggcgtgg ccagccccgg ggcggccgac tacgggctga gcaggttcga ccacatcgtc 660
ggcaacgtgc cggagctggc gcccgccgcc gcctacttcg ccggcttcac ggggttccac 720
gagttcgccg agttcacgac ggaggacgtg ggcaccgcgg agagcggcct caactccatg 780
gtgctcgcca acaactcgga gaacgtgctg ctcccgctca acgagccggt gcacggcacc 840
aagcgccgca gccagataca gacgttcctg gaccaccacg gcggccccgg cgtgcagcac 900
atggcgctgg ccagcgacga cgtgctcagg acgctgaggg agatgcaggc gcgctcggcc 960
atgggcggct tcgagttcat ggcgcctccc acatccgact actatgacgg cgtgaggcgg 1020
cgcgccgggg acgtgctcac ggaagcacag attaaggagt gccaggagct aggggtgctg 1080
gtggataggg atgaccaggg cgtgctgctc caaatcttcc ccaagccagt gggggacagg 1140
ccaacgctgt ggttggagat catccaaagg atcgggtgca tggagaggga tgagaagggg 1200
caagaatacc agaagggtgg ctgcggcggg ttcggcaagg gaaacttctc gcagctgttc 1260
aaatccatcg aggattatga gaagtccctt gaagccatgc aagctgctgc agcagctaca 1320
gctcagggat cctag 1335
<210> 139
<211> 1335
<212> DNA
<213> 人工序列-ZmHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 139
atgccaccca cacccacagc agcagcggcg ggcgcagcgg tcgcagcggc gtcagcggcg 60
gagcaagcgg cgttcagatt ggttggtcac cgcaacttcg tgagattcaa tcctcgctca 120
gatagattcc atactctggc cttccaccat gttgagcttt ggtgcgctga tgctgcttct 180
gctgctggaa ggttcagctt cggtttgggt gctcccctcg ctgctagatc tgatttgagc 240
actggtaact cagcacatgc ttccctcctg cttagatctg gctctctgag cttccttttc 300
actgctccat atgctcatgg tgctgatgct gctacagctg ctctcccttc attctccgct 360
gctgctgcta ggagattcgc tgctgatcac ggattggctg tgagggctgt tgcacttaga 420
gttgctgatg ctgaggaggc cttccgcacc tctgttgctg ctggagccag accagctttc 480
ggacctgttg atctcggaag gggtttccgc ctggctgagg tggagcttta cggcgatgtg 540
gttctgcgct acgttagcta tcctgatggt gctgctggtg aacctttcct tccaggattc 600
gagggtgtgg cctcaccagg tgccgctgat tacggcctgt ccagattcga ccacattgtt 660
ggaaatgttc ctgagcttgc tccagctgct gcctacttcg ctggcttcac cggattccat 720
gagttcgccg agttcaccac tgaggatgtg ggaactgctg agtctggttt gaatagcatg 780
gtgctcgcca acaattcaga gaacgttttg ctccctctga atgagcccgt gcacggaaca 840
aagagaaggt cccagattca aaccttcttg gatcaccacg gcggcccagg tgttcagcat 900
atggctctcg cctccgatga cgtgttgaga accctcaggg agatgcaagc caggtctgct 960
atgggtggct tcgagttcat ggccccacct acaagcgatt actatgatgg tgtgcgcaga 1020
agggctggcg acgttcttac cgaggctcag atcaaggagt gccaagagct gggcgtgctt 1080
gttgatagag atgaccaggg agttctgctt caaattttcc ccaagccagt gggcgacagg 1140
cctactttgt ggctcgagat tatccagaga atcggatgca tggagaggga tgagaagggt 1200
caggagtacc aaaagggagg ttgcggcgga ttcggcaagg gaaatttctc acaactcttc 1260
aagtccatcg aggactatga aaagagtctg gaagcgatgc aggcagcagc agcagcaacc 1320
gcacaggggt cgtga 1335
<210> 140
<211> 444
<212> PRT
<213> 人工序列-ZmHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 140
Met Pro Pro Thr Pro Thr Ala Ala Ala Ala Gly Ala Ala Val Ala Ala
1 5 10 15
Ala Ser Ala Ala Glu Gln Ala Ala Phe Arg Leu Val Gly His Arg Asn
20 25 30
Phe Val Arg Phe Asn Pro Arg Ser Asp Arg Phe His Thr Leu Ala Phe
35 40 45
His His Val Glu Leu Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg
50 55 60
Phe Ser Phe Gly Leu Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser
65 70 75 80
Thr Gly Asn Ser Ala His Ala Ser Leu Leu Leu Arg Ser Gly Ser Leu
85 90 95
Ser Phe Leu Phe Thr Ala Pro Tyr Ala His Gly Ala Asp Ala Ala Thr
100 105 110
Ala Ala Leu Pro Ser Phe Ser Ala Ala Ala Ala Arg Arg Phe Ala Ala
115 120 125
Asp His Gly Leu Ala Val Arg Ala Val Ala Leu Arg Val Ala Asp Ala
130 135 140
Glu Glu Ala Phe Arg Thr Ser Val Ala Ala Gly Ala Arg Pro Ala Phe
145 150 155 160
Gly Pro Val Asp Leu Gly Arg Gly Phe Arg Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr Pro Asp Gly Ala Ala
180 185 190
Gly Glu Pro Phe Leu Pro Gly Phe Glu Gly Val Ala Ser Pro Gly Ala
195 200 205
Ala Asp Tyr Gly Leu Ser Arg Phe Asp His Ile Val Gly Asn Val Pro
210 215 220
Glu Leu Ala Pro Ala Ala Ala Tyr Phe Ala Gly Phe Thr Gly Phe His
225 230 235 240
Glu Phe Ala Glu Phe Thr Thr Glu Asp Val Gly Thr Ala Glu Ser Gly
245 250 255
Leu Asn Ser Met Val Leu Ala Asn Asn Ser Glu Asn Val Leu Leu Pro
260 265 270
Leu Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr
275 280 285
Phe Leu Asp His His Gly Gly Pro Gly Val Gln His Met Ala Leu Ala
290 295 300
Ser Asp Asp Val Leu Arg Thr Leu Arg Glu Met Gln Ala Arg Ser Ala
305 310 315 320
Met Gly Gly Phe Glu Phe Met Ala Pro Pro Thr Ser Asp Tyr Tyr Asp
325 330 335
Gly Val Arg Arg Arg Ala Gly Asp Val Leu Thr Glu Ala Gln Ile Lys
340 345 350
Glu Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val
355 360 365
Leu Leu Gln Ile Ala Pro Lys Pro Val Gly Asp Arg Pro Thr Leu Trp
370 375 380
Leu Glu Ile Ile Gln Arg Ile Gly Cys Met Glu Arg Asp Glu Lys Gly
385 390 395 400
Gln Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe
405 410 415
Ser Gln Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Ala
420 425 430
Met Gln Ala Ala Ala Ala Ala Thr Ala Gln Gly Ser
435 440
<210> 141
<211> 1335
<212> DNA
<213> 人工序列-ZmHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 141
atgcccccga cccccacagc cgccgcagcc ggcgccgccg tggcggcggc atcagcagcg 60
gagcaggcgg cgttccgcct cgtgggccac cgcaacttcg tccgcttcaa cccgcgctcc 120
gaccgcttcc acacgctcgc gttccaccac gtggagctct ggtgcgccga cgcggcctcc 180
gccgcgggcc gcttctcctt cggcctgggc gcgccgctcg ccgcgcgctc cgacctctcc 240
acgggcaact ccgcgcacgc gtccctgctg ctccgctccg gctccctctc cttcctcttc 300
acggcgccct acgcgcacgg cgccgacgcc gccaccgctg cgctgccctc cttctccgcc 360
gccgccgcgc ggcgcttcgc ggccgaccac ggcctcgcgg tgcgcgccgt cgcgctccgc 420
gtcgccgacg ccgaggaggc cttccgcacc agcgtcgcgg ccggggcgcg cccggctttc 480
ggccccgtcg acctcggccg cggcttccgc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctccggt acgtgagcta cccggacggc gcggcgggcg agcccttcct gccggggttc 600
gagggcgtgg ccagccccgg ggcggccgac tacgggctga gcaggttcga ccacatcgtc 660
ggcaacgtgc cggagctggc gcccgccgcc gcctacttcg ccggcttcac ggggttccac 720
gagttcgccg agttcacgac ggaggacgtg ggcaccgcgg agagcggcct caactccatg 780
gtgctcgcca acaactcgga gaacgtgctg ctcccgctca acgagccggt gcacggcacc 840
aagcgccgca gccagataca gacgttcctg gaccaccacg gcggccccgg cgtgcagcac 900
atggcgctgg ccagcgacga cgtgctcagg acgctgaggg agatgcaggc gcgctcggcc 960
atgggcggct tcgagttcat ggcgcctccc acatccgact actatgacgg cgtgaggcgg 1020
cgcgccgggg acgtgctcac ggaagcacag attaaggagt gccaggagct aggggtgctg 1080
gtggataggg atgaccaggg cgtgctgctc caaatcgctc ccaagccagt gggggacagg 1140
ccaacgctgt ggttggagat catccaaagg atcgggtgca tggagaggga tgagaagggg 1200
caagaatacc agaagggtgg ctgcggcggg ttcggcaagg gaaacttctc gcagctgttc 1260
aaatccatcg aggattatga gaagtccctt gaagccatgc aagctgctgc agcagctaca 1320
gctcagggat cctag 1335
<210> 142
<211> 1335
<212> DNA
<213> 人工序列-ZmHPPDm-F372A-F383W-02核苷酸序列（Artificial Sequence)
<400> 142
atgccaccca cacccacagc agcagcggcg ggcgcagcgg tcgcagcggc gtcagcggcg 60
gagcaagcgg cgttcagatt ggttggtcac cgcaacttcg tgagattcaa tcctcgctca 120
gatagattcc atactctggc cttccaccat gttgagcttt ggtgcgctga tgctgcttct 180
gctgctggaa ggttcagctt cggtttgggt gctcccctcg ctgctagatc tgatttgagc 240
actggtaact cagcacatgc ttccctcctg cttagatctg gctctctgag cttccttttc 300
actgctccat atgctcatgg tgctgatgct gctacagctg ctctcccttc attctccgct 360
gctgctgcta ggagattcgc tgctgatcac ggattggctg tgagggctgt tgcacttaga 420
gttgctgatg ctgaggaggc cttccgcacc tctgttgctg ctggagccag accagctttc 480
ggacctgttg atctcggaag gggtttccgc ctggctgagg tggagcttta cggcgatgtg 540
gttctgcgct acgttagcta tcctgatggt gctgctggtg aacctttcct tccaggattc 600
gagggtgtgg cctcaccagg tgccgctgat tacggcctgt ccagattcga ccacattgtt 660
ggaaatgttc ctgagcttgc tccagctgct gcctacttcg ctggcttcac cggattccat 720
gagttcgccg agttcaccac tgaggatgtg ggaactgctg agtctggttt gaatagcatg 780
gtgctcgcca acaattcaga gaacgttttg ctccctctga atgagcccgt gcacggaaca 840
aagagaaggt cccagattca aaccttcttg gatcaccacg gcggcccagg tgttcagcat 900
atggctctcg cctccgatga cgtgttgaga accctcaggg agatgcaagc caggtctgct 960
atgggtggct tcgagttcat ggccccacct acaagcgatt actatgatgg tgtgcgcaga 1020
agggctggcg acgttcttac cgaggctcag atcaaggagt gccaagagct gggcgtgctt 1080
gttgatagag atgaccaggg agttctgctt caaattgctc ccaagccagt gggcgacagg 1140
cctactttgt ggctcgagat tatccagaga atcggatgca tggagaggga tgagaagggt 1200
caggagtacc aaaagggagg ttgcggcgga ttcggcaagg gaaatttctc acaactcttc 1260
aagtccatcg aggactatga aaagagtctg gaagcgatgc aggcagcagc agcagcaacc 1320
gcacaggggt cgtga 1335
<210> 143
<211> 358
<212> PRT
<213> 荧光假单胞菌野生型HPPD的氨基酸序列（Pseudomonas fluorescens)
<400> 143
Met Ala Asp Leu Tyr Glu Asn Pro Met Gly Leu Met Gly Phe Glu Phe
1 5 10 15
Ile Glu Phe Ala Ser Pro Thr Pro Gly Thr Leu Glu Pro Ile Phe Glu
20 25 30
Ile Met Gly Phe Thr Lys Val Ala Thr His Arg Ser Lys Asn Val His
35 40 45
Leu Tyr Arg Gln Gly Glu Ile Asn Leu Ile Leu Asn Asn Glu Pro Asn
50 55 60
Ser Ile Ala Ser Tyr Phe Ala Ala Glu His Gly Pro Ser Val Cys Gly
65 70 75 80
Met Ala Phe Arg Val Lys Asp Ser Gln Lys Ala Tyr Asn Arg Ala Leu
85 90 95
Glu Leu Gly Ala Gln Pro Ile His Ile Asp Thr Gly Pro Met Glu Leu
100 105 110
Asn Leu Pro Ala Ile Lys Gly Ile Gly Gly Ala Pro Leu Tyr Leu Ile
115 120 125
Asp Arg Phe Gly Glu Gly Ser Ser Ile Tyr Asp Ile Asp Phe Val Tyr
130 135 140
Leu Glu Gly Val Glu Arg Asn Pro Val Gly Ala Gly Leu Lys Val Ile
145 150 155 160
Asp His Leu Thr His Asn Val Tyr Arg Gly Arg Met Val Tyr Trp Ala
165 170 175
Asn Phe Tyr Glu Lys Leu Phe Asn Phe Arg Glu Ala Arg Tyr Phe Asp
180 185 190
Ile Lys Gly Glu Tyr Thr Gly Leu Thr Ser Lys Ala Met Ser Ala Pro
195 200 205
Asp Gly Met Ile Arg Ile Pro Leu Asn Glu Glu Ser Ser Lys Gly Ala
210 215 220
Gly Gln Ile Glu Glu Phe Leu Met Gln Phe Asn Gly Glu Gly Ile Gln
225 230 235 240
His Val Ala Phe Leu Thr Asp Asp Leu Val Lys Thr Trp Asp Ala Leu
245 250 255
Lys Lys Ile Gly Met Arg Phe Met Thr Ala Pro Pro Asp Thr Tyr Tyr
260 265 270
Glu Met Leu Glu Gly Arg Leu Pro Asp His Gly Glu Pro Val Asp Gln
275 280 285
Leu Gln Ala Arg Gly Ile Leu Leu Asp Gly Ser Ser Val Glu Gly Asp
290 295 300
Lys Arg Leu Leu Leu Gln Ile Phe Ser Glu Thr Leu Met Gly Pro Val
305 310 315 320
Phe Phe Glu Phe Ile Gln Arg Lys Gly Asp Asp Gly Phe Gly Glu Gly
325 330 335
Asn Phe Lys Ala Leu Phe Glu Ser Ile Glu Arg Asp Gln Val Arg Arg
340 345 350
Gly Val Leu Thr Ala Asp
355
<210> 144
<211> 1077
<212> DNA
<213> PfHPPD-01核苷酸序列(Pseudomonas fluorescens)
<400> 144
atggcagatc tatacgaaaa cccaatgggc ctgatgggct ttgaattcat cgaattcgcg 60
tcgccgacgc cgggcaccct ggagccgatc ttcgagatca tgggcttcac caaagtcgcg 120
acccaccgtt ccaagaacgt gcacctgtac cgccagggcg agatcaacct gatcctcaac 180
aacgagccca acagcatcgc ctcctacttt gcggccgaac acggcccgtc ggtgtgcggc 240
atggcgttcc gcgtgaagga ctcgcaaaag gcctacaacc gcgccctgga actcggcgcc 300
cagccgatcc atattgacac cgggccgatg gaattgaacc tgccggcgat caagggcatc 360
ggcggcgcgc cgttgtacct gatcgaccgt ttcggcgaag gcagctcgat ctacgacatc 420
gacttcgtgt acctcgaagg tgtggagcgc aatccggtcg gtgcaggtct caaagtcatc 480
gaccacctga cccacaacgt ctatcgcggc cgcatggtct actgggccaa cttctacgag 540
aaattgttca acttccgtga agcgcgttac ttcgatatca agggcgagta caccggcctg 600
acttccaagg ccatgagtgc gccggacggc atgatccgca tcccgctgaa cgaagagtcg 660
tccaagggcg cggggcagat cgaagagttc ctgatgcagt tcaacggcga aggcatccag 720
cacgtggcgt tcctcaccga cgacctggtc aagacctggg acgcgttgaa gaaaatcggc 780
atgcgcttca tgaccgcgcc gccagacact tattacgaaa tgctcgaagg ccgcctgcct 840
gaccacggcg agccggtgga tcaactgcag gcacgcggta tcctgctgga cggatcttcc 900
gtggaaggcg acaaacgcct gctgctgcag atcttctcgg aaaccctgat gggcccggtg 960
ttcttcgaat tcatccagcg caagggcgac gatgggtttg gcgagggcaa cttcaaggcg 1020
ctgttcgagt ccatcgaacg tgaccaggtg cgtcgtggtg tattgaccgc cgattaa 1077
<210> 145
<211> 1077
<212> DNA
<213> 人工序列-PfHPPD-02核苷酸序列(Artificial Sequence)
<400> 145
atggccgatt tgtacgagaa cccaatggga ctcatgggtt tcgagttcat tgagttcgct 60
tcccccaccc caggtacttt ggagcctatt ttcgagatca tgggcttcac taaggttgcc 120
acacacaggt ctaagaatgt gcatctctac cgccaaggag agattaacct gatccttaac 180
aatgagccta atagcatcgc ctcatacttc gctgctgagc atggtccatc tgtttgcggc 240
atggctttca gagtgaagga ttcacaaaag gcctacaaca gggctttgga gctcggcgcc 300
cagcccattc atatcgacac cggacctatg gagctgaatc ttcctgccat taagggcatc 360
ggcggagctc cattgtacct cattgatcgc ttcggcgagg gctcctctat ctacgatatc 420
gacttcgtgt acctggaggg cgttgagaga aaccctgtgg gagctggtct gaaggttatc 480
gaccacctta ctcataatgt ttacagagga aggatggtgt actgggccaa cttctacgag 540
aagctgttca atttccgcga ggctagatac ttcgatatta agggcgagta cacaggactt 600
acctccaagg ccatgtctgc tcccgacggt atgattagga tcccattgaa cgaggagagc 660
tcaaagggcg ccggacagat tgaggagttc ctcatgcaat tcaatggtga aggcatccag 720
cacgttgctt tcctgacaga tgaccttgtg aagacctggg atgccctcaa gaagattggc 780
atgaggttca tgaccgctcc acctgacact tactacgaga tgttggaggg acgcctccct 840
gatcatggcg agcccgttga ccagctgcaa gctagaggaa tcctcctgga tggttcctct 900
gtggagggcg acaagaggct tttgctccaa attttcagcg agactcttat gggacccgtg 960
ttcttcgagt tcatccagag gaagggcgat gacggattcg gcgagggcaa cttcaaggcc 1020
ctgttcgagt ctattgagag agatcaggtg aggcgcggcg ttcttacagc tgactga 1077
<210> 146
<211> 358
<212> PRT
<213> 人工序列-PfHPPDm-F372A氨基酸序列(Artificial Sequence)
<400> 146
Met Ala Asp Leu Tyr Glu Asn Pro Met Gly Leu Met Gly Phe Glu Phe
1 5 10 15
Ile Glu Phe Ala Ser Pro Thr Pro Gly Thr Leu Glu Pro Ile Phe Glu
20 25 30
Ile Met Gly Phe Thr Lys Val Ala Thr His Arg Ser Lys Asn Val His
35 40 45
Leu Tyr Arg Gln Gly Glu Ile Asn Leu Ile Leu Asn Asn Glu Pro Asn
50 55 60
Ser Ile Ala Ser Tyr Phe Ala Ala Glu His Gly Pro Ser Val Cys Gly
65 70 75 80
Met Ala Phe Arg Val Lys Asp Ser Gln Lys Ala Tyr Asn Arg Ala Leu
85 90 95
Glu Leu Gly Ala Gln Pro Ile His Ile Asp Thr Gly Pro Met Glu Leu
100 105 110
Asn Leu Pro Ala Ile Lys Gly Ile Gly Gly Ala Pro Leu Tyr Leu Ile
115 120 125
Asp Arg Phe Gly Glu Gly Ser Ser Ile Tyr Asp Ile Asp Phe Val Tyr
130 135 140
Leu Glu Gly Val Glu Arg Asn Pro Val Gly Ala Gly Leu Lys Val Ile
145 150 155 160
Asp His Leu Thr His Asn Val Tyr Arg Gly Arg Met Val Tyr Trp Ala
165 170 175
Asn Phe Tyr Glu Lys Leu Phe Asn Phe Arg Glu Ala Arg Tyr Phe Asp
180 185 190
Ile Lys Gly Glu Tyr Thr Gly Leu Thr Ser Lys Ala Met Ser Ala Pro
195 200 205
Asp Gly Met Ile Arg Ile Pro Leu Asn Glu Glu Ser Ser Lys Gly Ala
210 215 220
Gly Gln Ile Glu Glu Phe Leu Met Gln Phe Asn Gly Glu Gly Ile Gln
225 230 235 240
His Val Ala Phe Leu Thr Asp Asp Leu Val Lys Thr Trp Asp Ala Leu
245 250 255
Lys Lys Ile Gly Met Arg Phe Met Thr Ala Pro Pro Asp Thr Tyr Tyr
260 265 270
Glu Met Leu Glu Gly Arg Leu Pro Asp His Gly Glu Pro Val Asp Gln
275 280 285
Leu Gln Ala Arg Gly Ile Leu Leu Asp Gly Ser Ser Val Glu Gly Asp
290 295 300
Lys Arg Leu Leu Leu Gln Ile Ala Ser Glu Thr Leu Met Gly Pro Val
305 310 315 320
Phe Phe Glu Phe Ile Gln Arg Lys Gly Asp Asp Gly Phe Gly Glu Gly
325 330 335
Asn Phe Lys Ala Leu Phe Glu Ser Ile Glu Arg Asp Gln Val Arg Arg
340 345 350
Gly Val Leu Thr Ala Asp
355
<210> 147
<211> 1077
<212> DNA
<213> 人工序列-PfHPPDm-F372A-01核苷酸序列(Artificial Sequence)
<400> 147
atggcagatc tatacgaaaa cccaatgggc ctgatgggct ttgaattcat cgaattcgcg 60
tcgccgacgc cgggcaccct ggagccgatc ttcgagatca tgggcttcac caaagtcgcg 120
acccaccgtt ccaagaacgt gcacctgtac cgccagggcg agatcaacct gatcctcaac 180
aacgagccca acagcatcgc ctcctacttt gcggccgaac acggcccgtc ggtgtgcggc 240
atggcgttcc gcgtgaagga ctcgcaaaag gcctacaacc gcgccctgga actcggcgcc 300
cagccgatcc atattgacac cgggccgatg gaattgaacc tgccggcgat caagggcatc 360
ggcggcgcgc cgttgtacct gatcgaccgt ttcggcgaag gcagctcgat ctacgacatc 420
gacttcgtgt acctcgaagg tgtggagcgc aatccggtcg gtgcaggtct caaagtcatc 480
gaccacctga cccacaacgt ctatcgcggc cgcatggtct actgggccaa cttctacgag 540
aaattgttca acttccgtga agcgcgttac ttcgatatca agggcgagta caccggcctg 600
acttccaagg ccatgagtgc gccggacggc atgatccgca tcccgctgaa cgaagagtcg 660
tccaagggcg cggggcagat cgaagagttc ctgatgcagt tcaacggcga aggcatccag 720
cacgtggcgt tcctcaccga cgacctggtc aagacctggg acgcgttgaa gaaaatcggc 780
atgcgcttca tgaccgcgcc gccagacact tattacgaaa tgctcgaagg ccgcctgcct 840
gaccacggcg agccggtgga tcaactgcag gcacgcggta tcctgctgga cggatcttcc 900
gtggaaggcg acaaacgcct gctgctgcag atcgcttcgg aaaccctgat gggcccggtg 960
ttcttcgaat tcatccagcg caagggcgac gatgggtttg gcgagggcaa cttcaaggcg 1020
ctgttcgagt ccatcgaacg tgaccaggtg cgtcgtggtg tattgaccgc cgattaa 1077
<210> 148
<211> 1077
<212> DNA
<213> 人工序列-PfHPPDm-F372A-02核苷酸序列(Artificial Sequence)
<400> 148
atggccgatt tgtacgagaa cccaatggga ctcatgggtt tcgagttcat tgagttcgct 60
tcccccaccc caggtacttt ggagcctatt ttcgagatca tgggcttcac taaggttgcc 120
acacacaggt ctaagaatgt gcatctctac cgccaaggag agattaacct gatccttaac 180
aatgagccta atagcatcgc ctcatacttc gctgctgagc atggtccatc tgtttgcggc 240
atggctttca gagtgaagga ttcacaaaag gcctacaaca gggctttgga gctcggcgcc 300
cagcccattc atatcgacac cggacctatg gagctgaatc ttcctgccat taagggcatc 360
ggcggagctc cattgtacct cattgatcgc ttcggcgagg gctcctctat ctacgatatc 420
gacttcgtgt acctggaggg cgttgagaga aaccctgtgg gagctggtct gaaggttatc 480
gaccacctta ctcataatgt ttacagagga aggatggtgt actgggccaa cttctacgag 540
aagctgttca atttccgcga ggctagatac ttcgatatta agggcgagta cacaggactt 600
acctccaagg ccatgtctgc tcccgacggt atgattagga tcccattgaa cgaggagagc 660
tcaaagggcg ccggacagat tgaggagttc ctcatgcaat tcaatggtga aggcatccag 720
cacgttgctt tcctgacaga tgaccttgtg aagacctggg atgccctcaa gaagattggc 780
atgaggttca tgaccgctcc acctgacact tactacgaga tgttggaggg acgcctccct 840
gatcatggcg agcccgttga ccagctgcaa gctagaggaa tcctcctgga tggttcctct 900
gtggagggcg acaagaggct tttgctccaa attgctagcg agactcttat gggacccgtg 960
ttcttcgagt tcatccagag gaagggcgat gacggattcg gcgagggcaa cttcaaggcc 1020
ctgttcgagt ctattgagag agatcaggtg aggcgcggcg ttcttacagc tgactga 1077
<210> 149
<211> 358
<212> PRT
<213> 人工序列-PfHPPDm-F383W氨基酸序列(Artificial Sequence)
<400> 149
Met Ala Asp Leu Tyr Glu Asn Pro Met Gly Leu Met Gly Phe Glu Phe
1 5 10 15
Ile Glu Phe Ala Ser Pro Thr Pro Gly Thr Leu Glu Pro Ile Phe Glu
20 25 30
Ile Met Gly Phe Thr Lys Val Ala Thr His Arg Ser Lys Asn Val His
35 40 45
Leu Tyr Arg Gln Gly Glu Ile Asn Leu Ile Leu Asn Asn Glu Pro Asn
50 55 60
Ser Ile Ala Ser Tyr Phe Ala Ala Glu His Gly Pro Ser Val Cys Gly
65 70 75 80
Met Ala Phe Arg Val Lys Asp Ser Gln Lys Ala Tyr Asn Arg Ala Leu
85 90 95
Glu Leu Gly Ala Gln Pro Ile His Ile Asp Thr Gly Pro Met Glu Leu
100 105 110
Asn Leu Pro Ala Ile Lys Gly Ile Gly Gly Ala Pro Leu Tyr Leu Ile
115 120 125
Asp Arg Phe Gly Glu Gly Ser Ser Ile Tyr Asp Ile Asp Phe Val Tyr
130 135 140
Leu Glu Gly Val Glu Arg Asn Pro Val Gly Ala Gly Leu Lys Val Ile
145 150 155 160
Asp His Leu Thr His Asn Val Tyr Arg Gly Arg Met Val Tyr Trp Ala
165 170 175
Asn Phe Tyr Glu Lys Leu Phe Asn Phe Arg Glu Ala Arg Tyr Phe Asp
180 185 190
Ile Lys Gly Glu Tyr Thr Gly Leu Thr Ser Lys Ala Met Ser Ala Pro
195 200 205
Asp Gly Met Ile Arg Ile Pro Leu Asn Glu Glu Ser Ser Lys Gly Ala
210 215 220
Gly Gln Ile Glu Glu Phe Leu Met Gln Phe Asn Gly Glu Gly Ile Gln
225 230 235 240
His Val Ala Phe Leu Thr Asp Asp Leu Val Lys Thr Trp Asp Ala Leu
245 250 255
Lys Lys Ile Gly Met Arg Phe Met Thr Ala Pro Pro Asp Thr Tyr Tyr
260 265 270
Glu Met Leu Glu Gly Arg Leu Pro Asp His Gly Glu Pro Val Asp Gln
275 280 285
Leu Gln Ala Arg Gly Ile Leu Leu Asp Gly Ser Ser Val Glu Gly Asp
290 295 300
Lys Arg Leu Leu Leu Gln Ile Phe Ser Glu Thr Leu Met Gly Pro Val
305 310 315 320
Trp Phe Glu Phe Ile Gln Arg Lys Gly Asp Asp Gly Phe Gly Glu Gly
325 330 335
Asn Phe Lys Ala Leu Phe Glu Ser Ile Glu Arg Asp Gln Val Arg Arg
340 345 350
Gly Val Leu Thr Ala Asp
355
<210> 150
<211> 1077
<212> DNA
<213> 人工序列-PfHPPDm-F383W-01核苷酸序列(Artificial Sequence)
<400> 150
atggcagatc tatacgaaaa cccaatgggc ctgatgggct ttgaattcat cgaattcgcg 60
tcgccgacgc cgggcaccct ggagccgatc ttcgagatca tgggcttcac caaagtcgcg 120
acccaccgtt ccaagaacgt gcacctgtac cgccagggcg agatcaacct gatcctcaac 180
aacgagccca acagcatcgc ctcctacttt gcggccgaac acggcccgtc ggtgtgcggc 240
atggcgttcc gcgtgaagga ctcgcaaaag gcctacaacc gcgccctgga actcggcgcc 300
cagccgatcc atattgacac cgggccgatg gaattgaacc tgccggcgat caagggcatc 360
ggcggcgcgc cgttgtacct gatcgaccgt ttcggcgaag gcagctcgat ctacgacatc 420
gacttcgtgt acctcgaagg tgtggagcgc aatccggtcg gtgcaggtct caaagtcatc 480
gaccacctga cccacaacgt ctatcgcggc cgcatggtct actgggccaa cttctacgag 540
aaattgttca acttccgtga agcgcgttac ttcgatatca agggcgagta caccggcctg 600
acttccaagg ccatgagtgc gccggacggc atgatccgca tcccgctgaa cgaagagtcg 660
tccaagggcg cggggcagat cgaagagttc ctgatgcagt tcaacggcga aggcatccag 720
cacgtggcgt tcctcaccga cgacctggtc aagacctggg acgcgttgaa gaaaatcggc 780
atgcgcttca tgaccgcgcc gccagacact tattacgaaa tgctcgaagg ccgcctgcct 840
gaccacggcg agccggtgga tcaactgcag gcacgcggta tcctgctgga cggatcttcc 900
gtggaaggcg acaaacgcct gctgctgcag atcttctcgg aaaccctgat gggcccggtg 960
tggttcgaat tcatccagcg caagggcgac gatgggtttg gcgagggcaa cttcaaggcg 1020
ctgttcgagt ccatcgaacg tgaccaggtg cgtcgtggtg tattgaccgc cgattaa 1077
<210> 151
<211> 1077
<212> DNA
<213> 人工序列-PfHPPDm-F383W-02核苷酸序列(Artificial Sequence)
<400> 151
atggccgatt tgtacgagaa cccaatggga ctcatgggtt tcgagttcat tgagttcgct 60
tcccccaccc caggtacttt ggagcctatt ttcgagatca tgggcttcac taaggttgcc 120
acacacaggt ctaagaatgt gcatctctac cgccaaggag agattaacct gatccttaac 180
aatgagccta atagcatcgc ctcatacttc gctgctgagc atggtccatc tgtttgcggc 240
atggctttca gagtgaagga ttcacaaaag gcctacaaca gggctttgga gctcggcgcc 300
cagcccattc atatcgacac cggacctatg gagctgaatc ttcctgccat taagggcatc 360
ggcggagctc cattgtacct cattgatcgc ttcggcgagg gctcctctat ctacgatatc 420
gacttcgtgt acctggaggg cgttgagaga aaccctgtgg gagctggtct gaaggttatc 480
gaccacctta ctcataatgt ttacagagga aggatggtgt actgggccaa cttctacgag 540
aagctgttca atttccgcga ggctagatac ttcgatatta agggcgagta cacaggactt 600
acctccaagg ccatgtctgc tcccgacggt atgattagga tcccattgaa cgaggagagc 660
tcaaagggcg ccggacagat tgaggagttc ctcatgcaat tcaatggtga aggcatccag 720
cacgttgctt tcctgacaga tgaccttgtg aagacctggg atgccctcaa gaagattggc 780
atgaggttca tgaccgctcc acctgacact tactacgaga tgttggaggg acgcctccct 840
gatcatggcg agcccgttga ccagctgcaa gctagaggaa tcctcctgga tggttcctct 900
gtggagggcg acaagaggct tttgctccaa attttcagcg agactcttat gggacccgtg 960
tggttcgagt tcatccagag gaagggcgat gacggattcg gcgagggcaa cttcaaggcc 1020
ctgttcgagt ctattgagag agatcaggtg aggcgcggcg ttcttacagc tgactga 1077
<210> 152
<211> 358
<212> PRT
<213> 人工序列-PfHPPDm-F372A-F383W氨基酸序列(Artificial Sequence)
<400> 152
Met Ala Asp Leu Tyr Glu Asn Pro Met Gly Leu Met Gly Phe Glu Phe
1 5 10 15
Ile Glu Phe Ala Ser Pro Thr Pro Gly Thr Leu Glu Pro Ile Phe Glu
20 25 30
Ile Met Gly Phe Thr Lys Val Ala Thr His Arg Ser Lys Asn Val His
35 40 45
Leu Tyr Arg Gln Gly Glu Ile Asn Leu Ile Leu Asn Asn Glu Pro Asn
50 55 60
Ser Ile Ala Ser Tyr Phe Ala Ala Glu His Gly Pro Ser Val Cys Gly
65 70 75 80
Met Ala Phe Arg Val Lys Asp Ser Gln Lys Ala Tyr Asn Arg Ala Leu
85 90 95
Glu Leu Gly Ala Gln Pro Ile His Ile Asp Thr Gly Pro Met Glu Leu
100 105 110
Asn Leu Pro Ala Ile Lys Gly Ile Gly Gly Ala Pro Leu Tyr Leu Ile
115 120 125
Asp Arg Phe Gly Glu Gly Ser Ser Ile Tyr Asp Ile Asp Phe Val Tyr
130 135 140
Leu Glu Gly Val Glu Arg Asn Pro Val Gly Ala Gly Leu Lys Val Ile
145 150 155 160
Asp His Leu Thr His Asn Val Tyr Arg Gly Arg Met Val Tyr Trp Ala
165 170 175
Asn Phe Tyr Glu Lys Leu Phe Asn Phe Arg Glu Ala Arg Tyr Phe Asp
180 185 190
Ile Lys Gly Glu Tyr Thr Gly Leu Thr Ser Lys Ala Met Ser Ala Pro
195 200 205
Asp Gly Met Ile Arg Ile Pro Leu Asn Glu Glu Ser Ser Lys Gly Ala
210 215 220
Gly Gln Ile Glu Glu Phe Leu Met Gln Phe Asn Gly Glu Gly Ile Gln
225 230 235 240
His Val Ala Phe Leu Thr Asp Asp Leu Val Lys Thr Trp Asp Ala Leu
245 250 255
Lys Lys Ile Gly Met Arg Phe Met Thr Ala Pro Pro Asp Thr Tyr Tyr
260 265 270
Glu Met Leu Glu Gly Arg Leu Pro Asp His Gly Glu Pro Val Asp Gln
275 280 285
Leu Gln Ala Arg Gly Ile Leu Leu Asp Gly Ser Ser Val Glu Gly Asp
290 295 300
Lys Arg Leu Leu Leu Gln Ile Ala Ser Glu Thr Leu Met Gly Pro Val
305 310 315 320
Trp Phe Glu Phe Ile Gln Arg Lys Gly Asp Asp Gly Phe Gly Glu Gly
325 330 335
Asn Phe Lys Ala Leu Phe Glu Ser Ile Glu Arg Asp Gln Val Arg Arg
340 345 350
Gly Val Leu Thr Ala Asp
355
<210> 153
<211> 1077
<212> DNA
<213> 人工序列-PfHPPDm-F372A-F383W-01核苷酸序列(Artificial Sequence)
<400> 153
atggcagatc tatacgaaaa cccaatgggc ctgatgggct ttgaattcat cgaattcgcg 60
tcgccgacgc cgggcaccct ggagccgatc ttcgagatca tgggcttcac caaagtcgcg 120
acccaccgtt ccaagaacgt gcacctgtac cgccagggcg agatcaacct gatcctcaac 180
aacgagccca acagcatcgc ctcctacttt gcggccgaac acggcccgtc ggtgtgcggc 240
atggcgttcc gcgtgaagga ctcgcaaaag gcctacaacc gcgccctgga actcggcgcc 300
cagccgatcc atattgacac cgggccgatg gaattgaacc tgccggcgat caagggcatc 360
ggcggcgcgc cgttgtacct gatcgaccgt ttcggcgaag gcagctcgat ctacgacatc 420
gacttcgtgt acctcgaagg tgtggagcgc aatccggtcg gtgcaggtct caaagtcatc 480
gaccacctga cccacaacgt ctatcgcggc cgcatggtct actgggccaa cttctacgag 540
aaattgttca acttccgtga agcgcgttac ttcgatatca agggcgagta caccggcctg 600
acttccaagg ccatgagtgc gccggacggc atgatccgca tcccgctgaa cgaagagtcg 660
tccaagggcg cggggcagat cgaagagttc ctgatgcagt tcaacggcga aggcatccag 720
cacgtggcgt tcctcaccga cgacctggtc aagacctggg acgcgttgaa gaaaatcggc 780
atgcgcttca tgaccgcgcc gccagacact tattacgaaa tgctcgaagg ccgcctgcct 840
gaccacggcg agccggtgga tcaactgcag gcacgcggta tcctgctgga cggatcttcc 900
gtggaaggcg acaaacgcct gctgctgcag atcgcttcgg aaaccctgat gggcccggtg 960
tggttcgaat tcatccagcg caagggcgac gatgggtttg gcgagggcaa cttcaaggcg 1020
ctgttcgagt ccatcgaacg tgaccaggtg cgtcgtggtg tattgaccgc cgattaa 1077
<210> 154
<211> 1077
<212> DNA
<213> 人工序列-PfHPPDm-F372A-F383W-02核苷酸序列(Artificial Sequence)
<400> 154
atggccgatt tgtacgagaa cccaatggga ctcatgggtt tcgagttcat tgagttcgct 60
tcccccaccc caggtacttt ggagcctatt ttcgagatca tgggcttcac taaggttgcc 120
acacacaggt ctaagaatgt gcatctctac cgccaaggag agattaacct gatccttaac 180
aatgagccta atagcatcgc ctcatacttc gctgctgagc atggtccatc tgtttgcggc 240
atggctttca gagtgaagga ttcacaaaag gcctacaaca gggctttgga gctcggcgcc 300
cagcccattc atatcgacac cggacctatg gagctgaatc ttcctgccat taagggcatc 360
ggcggagctc cattgtacct cattgatcgc ttcggcgagg gctcctctat ctacgatatc 420
gacttcgtgt acctggaggg cgttgagaga aaccctgtgg gagctggtct gaaggttatc 480
gaccacctta ctcataatgt ttacagagga aggatggtgt actgggccaa cttctacgag 540
aagctgttca atttccgcga ggctagatac ttcgatatta agggcgagta cacaggactt 600
acctccaagg ccatgtctgc tcccgacggt atgattagga tcccattgaa cgaggagagc 660
tcaaagggcg ccggacagat tgaggagttc ctcatgcaat tcaatggtga aggcatccag 720
cacgttgctt tcctgacaga tgaccttgtg aagacctggg atgccctcaa gaagattggc 780
atgaggttca tgaccgctcc acctgacact tactacgaga tgttggaggg acgcctccct 840
gatcatggcg agcccgttga ccagctgcaa gctagaggaa tcctcctgga tggttcctct 900
gtggagggcg acaagaggct tttgctccaa attgctagcg agactcttat gggacccgtg 960
tggttcgagt tcatccagag gaagggcgat gacggattcg gcgagggcaa cttcaaggcc 1020
ctgttcgagt ctattgagag agatcaggtg aggcgcggcg ttcttacagc tgactga 1077
<210> 155
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F372G氨基酸序列(Artificial Sequence)
<400> 155
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Gly Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Phe Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 156
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372G-01核苷酸序列(Artificial Sequence)
<400> 156
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcggtacca agccagtagg ggacaggcca 1140
acgtttttcc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 157
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372G-02核苷酸序列(Artificial Sequence)
<400> 157
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attggtacta agccagtggg cgaccgccct 1140
acattcttcc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 158
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F372G-F383W氨基酸序列(Artificial Sequence)
<400> 158
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Gly Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 159
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372G-F383W-01核苷酸序列(Artificial Sequence)
<400> 159
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcggtacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 160
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372G-F383W-02核苷酸序列(Artificial Sequence)
<400> 160
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attggtacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 161
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F372V氨基酸序列(Artificial Sequence)
<400> 161
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Val Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Phe Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 162
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372V-01核苷酸序列(Artificial Sequence)
<400> 162
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgtcacca agccagtagg ggacaggcca 1140
acgtttttcc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 163
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372V-02核苷酸序列(Artificial Sequence)
<400> 163
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgtcacta agccagtggg cgaccgccct 1140
acattcttcc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 164
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F372V-F383W氨基酸序列(Artificial Sequence)
<400> 164
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Val Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 165
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372V-F383W-01核苷酸序列(Artificial Sequence)
<400> 165
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgtcacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 166
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372V-F383W-02核苷酸序列(Artificial Sequence)
<400> 166
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgtcacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 167
<211> 439
<212> PRT
<213> 人工序列-AsHPPDm-A107-F372A氨基酸序列(Artificial Sequence)
<400> 167
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Thr Ala Ala Ala Thr Ala
100 105 110
Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala Ala
115 120 125
His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala Ala
130 135 140
Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe Ala
145 150 155 160
Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu Tyr
165 170 175
Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp Leu
180 185 190
Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val Asp
195 200 205
Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu Met
210 215 220
Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu Phe
225 230 235 240
Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu Asn
245 250 255
Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu Asn
260 265 270
Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr Leu
275 280 285
Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser Asn
290 295 300
Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met Gly
305 310 315 320
Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly Val
325 330 335
Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu Cys
340 345 350
Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu Leu
355 360 365
Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Phe Leu Glu
370 375 380
Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln Glu
385 390 395 400
Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu
405 410 415
Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys Gln
420 425 430
Ser Val Val Ala Gln Lys Ser
435
<210> 168
<211> 1320
<212> DNA
<213> 人工序列-AsHPPDm-A107-F372A-01核苷酸序列(Artificial Sequence)
<400> 168
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccac ggccgcagcc accgcctcca tcccctcctt ctccgccgac 360
gccgcgcgga cgttcgccgc cgcccacggc ctcgcggtgc gctccgtcgg ggtccgcgtc 420
gctgacgccg ccgaggcctt ccgcgtcagc gtagccggcg gcgctcgccc ggccttcgcc 480
ccagccgacc tcggccatgg cttcggcctc gccgaggtcg agctctacgg cgacgtcgtg 540
ctacgcttcg tcagctaccc ggacgagaca gacctgccat tcctgccagg gttcgagcgc 600
gtgagcagcc ccggcgccgt ggactacggc ctcacgcggt tcgaccacgt cgtgggcaac 660
gtcccggaga tggccccggt catagactac atgaaaggct tcttggggtt ccacgagttc 720
gccgagttca ccgccgagga cgtgggcacg accgagagcg ggctcaactc ggtggtgctc 780
gccaacaact ccgaggccgt gctgctgccg ctcaacgagc ccgtgcacgg cacaaagcga 840
cggagccaga tacagacgta cctggagtat cacggcgggc ccggcgtgca gcacatcgcg 900
ctcgccagca acgacgtgct caggacgctc agggagatgc gggcgcgcac gcccatgggc 960
ggcttcgagt tcatggcgcc accgcaggcg aaatactatg aaggcgtgcg gcgcatcgca 1020
ggtgacgtgc tctcggaaga gcagatcaag gaatgccagg agctgggggt gctagtcgac 1080
agggatgatc aaggggtgtt gctccaaatc gctaccaagc cagtagggga caggccaacg 1140
tttttcctgg agatgatcca aagaatcggg tgcatggaga aggacgaggt cgggcaagag 1200
taccagaagg gtggctgcgg cgggtttggc aagggcaatt tctccgagct gttcaagtcc 1260
attgaggact atgagaaatc ccttgaggtc aagcaatctg ttgtagctca gaaatcctag 1320
<210> 169
<211> 1320
<212> DNA
<213> 人工序列-AsHPPDm-A107-F372A-02核苷酸序列(Artificial Sequence)
<400> 169
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctac tgctgctgct acagcctcca ttccaagctt ctcagctgat 360
gccgctagaa ccttcgctgc tgctcatgga cttgccgtga ggtctgttgg tgttcgcgtg 420
gctgatgctg ctgaggcttt cagagtttcc gttgccggcg gagctaggcc agctttcgct 480
cctgctgatt tgggacatgg tttcggcctg gctgaggttg agctttacgg tgacgtggtt 540
ctccgcttcg tgtcttaccc agatgagact gacctgcctt tccttcccgg cttcgagaga 600
gtttcctctc ctggtgccgt ggattacggc ctgacaaggt tcgaccacgt ggttggaaac 660
gttcccgaga tggctccagt gatcgattac atgaagggct tccttggatt ccatgagttc 720
gccgagttca ctgctgagga cgtgggaacc actgagtccg gtttgaactc tgtggttctc 780
gccaacaatt ctgaggctgt tttgctccct ctgaatgagc ctgtgcatgg aaccaagagg 840
agaagccaga ttcaaactta cctggagtac cacggtggcc ctggagttca gcatatcgcc 900
cttgcttcta atgatgtgtt gcgcacactc agagagatgc gcgccagaac ccccatggga 960
ggtttcgagt tcatggcccc accacaggct aagtactacg agggcgttag aaggattgct 1020
ggagacgtgt tgagcgagga gcagatcaag gagtgccaag agctgggtgt tcttgtggat 1080
agggatgacc agggcgttct gcttcaaatt gccactaagc cagtgggcga ccgccctaca 1140
ttcttccttg agatgattca aagaatcggc tgcatggaga aggatgaggt tggacaggag 1200
taccaaaagg gcggatgcgg tggcttcgga aagggtaact tcagcgagtt gttcaagtca 1260
atcgaggact acgagaagag cctcgaggtg aagcagtcag tggttgccca aaagtcctga 1320
<210> 170
<211> 439
<212> PRT
<213> 人工序列-AsHPPDm-A107-F383W氨基酸序列(Artificial Sequence)
<400> 170
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Thr Ala Ala Ala Thr Ala
100 105 110
Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala Ala
115 120 125
His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala Ala
130 135 140
Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe Ala
145 150 155 160
Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu Tyr
165 170 175
Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp Leu
180 185 190
Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val Asp
195 200 205
Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu Met
210 215 220
Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu Phe
225 230 235 240
Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu Asn
245 250 255
Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu Asn
260 265 270
Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr Leu
275 280 285
Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser Asn
290 295 300
Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met Gly
305 310 315 320
Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly Val
325 330 335
Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu Cys
340 345 350
Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu Leu
355 360 365
Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu Glu
370 375 380
Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln Glu
385 390 395 400
Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu
405 410 415
Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys Gln
420 425 430
Ser Val Val Ala Gln Lys Ser
435
<210> 171
<211> 1320
<212> DNA
<213> 人工序列-AsHPPDm-A107-F383W-01核苷酸序列(Artificial Sequence)
<400> 171
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccac ggccgcagcc accgcctcca tcccctcctt ctccgccgac 360
gccgcgcgga cgttcgccgc cgcccacggc ctcgcggtgc gctccgtcgg ggtccgcgtc 420
gctgacgccg ccgaggcctt ccgcgtcagc gtagccggcg gcgctcgccc ggccttcgcc 480
ccagccgacc tcggccatgg cttcggcctc gccgaggtcg agctctacgg cgacgtcgtg 540
ctacgcttcg tcagctaccc ggacgagaca gacctgccat tcctgccagg gttcgagcgc 600
gtgagcagcc ccggcgccgt ggactacggc ctcacgcggt tcgaccacgt cgtgggcaac 660
gtcccggaga tggccccggt catagactac atgaaaggct tcttggggtt ccacgagttc 720
gccgagttca ccgccgagga cgtgggcacg accgagagcg ggctcaactc ggtggtgctc 780
gccaacaact ccgaggccgt gctgctgccg ctcaacgagc ccgtgcacgg cacaaagcga 840
cggagccaga tacagacgta cctggagtat cacggcgggc ccggcgtgca gcacatcgcg 900
ctcgccagca acgacgtgct caggacgctc agggagatgc gggcgcgcac gcccatgggc 960
ggcttcgagt tcatggcgcc accgcaggcg aaatactatg aaggcgtgcg gcgcatcgca 1020
ggtgacgtgc tctcggaaga gcagatcaag gaatgccagg agctgggggt gctagtcgac 1080
agggatgatc aaggggtgtt gctccaaatc ttcaccaagc cagtagggga caggccaacg 1140
ttttggctgg agatgatcca aagaatcggg tgcatggaga aggacgaggt cgggcaagag 1200
taccagaagg gtggctgcgg cgggtttggc aagggcaatt tctccgagct gttcaagtcc 1260
attgaggact atgagaaatc ccttgaggtc aagcaatctg ttgtagctca gaaatcctag 1320
<210> 172
<211> 1320
<212> DNA
<213> 人工序列-AsHPPDm-A107-F383W-02核苷酸序列(Artificial Sequence)
<400> 172
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctac tgctgctgct acagcctcca ttccaagctt ctcagctgat 360
gccgctagaa ccttcgctgc tgctcatgga cttgccgtga ggtctgttgg tgttcgcgtg 420
gctgatgctg ctgaggcttt cagagtttcc gttgccggcg gagctaggcc agctttcgct 480
cctgctgatt tgggacatgg tttcggcctg gctgaggttg agctttacgg tgacgtggtt 540
ctccgcttcg tgtcttaccc agatgagact gacctgcctt tccttcccgg cttcgagaga 600
gtttcctctc ctggtgccgt ggattacggc ctgacaaggt tcgaccacgt ggttggaaac 660
gttcccgaga tggctccagt gatcgattac atgaagggct tccttggatt ccatgagttc 720
gccgagttca ctgctgagga cgtgggaacc actgagtccg gtttgaactc tgtggttctc 780
gccaacaatt ctgaggctgt tttgctccct ctgaatgagc ctgtgcatgg aaccaagagg 840
agaagccaga ttcaaactta cctggagtac cacggtggcc ctggagttca gcatatcgcc 900
cttgcttcta atgatgtgtt gcgcacactc agagagatgc gcgccagaac ccccatggga 960
ggtttcgagt tcatggcccc accacaggct aagtactacg agggcgttag aaggattgct 1020
ggagacgtgt tgagcgagga gcagatcaag gagtgccaag agctgggtgt tcttgtggat 1080
agggatgacc agggcgttct gcttcaaatt ttcactaagc cagtgggcga ccgccctaca 1140
ttctggcttg agatgattca aagaatcggc tgcatggaga aggatgaggt tggacaggag 1200
taccaaaagg gcggatgcgg tggcttcgga aagggtaact tcagcgagtt gttcaagtca 1260
atcgaggact acgagaagag cctcgaggtg aagcagtcag tggttgccca aaagtcctga 1320
<210> 173
<211> 439
<212> PRT
<213> 人工序列-HPPDm-1氨基酸序列(Artificial Sequence)
<400> 173
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Thr Ala Ala Ala Thr Ala
100 105 110
Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala Ala
115 120 125
His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala Ala
130 135 140
Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe Ala
145 150 155 160
Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu Tyr
165 170 175
Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp Leu
180 185 190
Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val Asp
195 200 205
Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu Met
210 215 220
Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu Phe
225 230 235 240
Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu Asn
245 250 255
Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu Asn
260 265 270
Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr Leu
275 280 285
Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser Asn
290 295 300
Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met Gly
305 310 315 320
Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly Val
325 330 335
Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu Cys
340 345 350
Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu Leu
355 360 365
Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu Glu
370 375 380
Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln Glu
385 390 395 400
Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu
405 410 415
Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys Gln
420 425 430
Ser Val Val Ala Gln Lys Ser
435
<210> 174
<211> 1320
<212> DNA
<213> 人工序列-HPPDm-1-01核苷酸序列(Artificial Sequence)
<400> 174
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccac ggccgcagcc accgcctcca tcccctcctt ctccgccgac 360
gccgcgcgga cgttcgccgc cgcccacggc ctcgcggtgc gctccgtcgg ggtccgcgtc 420
gctgacgccg ccgaggcctt ccgcgtcagc gtagccggcg gcgctcgccc ggccttcgcc 480
ccagccgacc tcggccatgg cttcggcctc gccgaggtcg agctctacgg cgacgtcgtg 540
ctacgcttcg tcagctaccc ggacgagaca gacctgccat tcctgccagg gttcgagcgc 600
gtgagcagcc ccggcgccgt ggactacggc ctcacgcggt tcgaccacgt cgtgggcaac 660
gtcccggaga tggccccggt catagactac atgaaaggct tcttggggtt ccacgagttc 720
gccgagttca ccgccgagga cgtgggcacg accgagagcg ggctcaactc ggtggtgctc 780
gccaacaact ccgaggccgt gctgctgccg ctcaacgagc ccgtgcacgg cacaaagcga 840
cggagccaga tacagacgta cctggagtat cacggcgggc ccggcgtgca gcacatcgcg 900
ctcgccagca acgacgtgct caggacgctc agggagatgc gggcgcgcac gcccatgggc 960
ggcttcgagt tcatggcgcc accgcaggcg aaatactatg aaggcgtgcg gcgcatcgca 1020
ggtgacgtgc tctcggaaga gcagatcaag gaatgccagg agctgggggt gctagtcgac 1080
agggatgatc aaggggtgtt gctccaaatc gctaccaagc cagtagggga caggccaacg 1140
ttttggctgg agatgatcca aagaatcggg tgcatggaga aggacgaggt cgggcaagag 1200
taccagaagg gtggctgcgg cgggtttggc aagggcaatt tctccgagct gttcaagtcc 1260
attgaggact atgagaaatc ccttgaggtc aagcaatctg ttgtagctca gaaatcctag 1320
<210> 175
<211> 1320
<212> DNA
<213> 人工序列-HPPDm-1-02核苷酸序列(Artificial Sequence)
<400> 175
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctac tgctgctgct acagcctcca ttccaagctt ctcagctgat 360
gccgctagaa ccttcgctgc tgctcatgga cttgccgtga ggtctgttgg tgttcgcgtg 420
gctgatgctg ctgaggcttt cagagtttcc gttgccggcg gagctaggcc agctttcgct 480
cctgctgatt tgggacatgg tttcggcctg gctgaggttg agctttacgg tgacgtggtt 540
ctccgcttcg tgtcttaccc agatgagact gacctgcctt tccttcccgg cttcgagaga 600
gtttcctctc ctggtgccgt ggattacggc ctgacaaggt tcgaccacgt ggttggaaac 660
gttcccgaga tggctccagt gatcgattac atgaagggct tccttggatt ccatgagttc 720
gccgagttca ctgctgagga cgtgggaacc actgagtccg gtttgaactc tgtggttctc 780
gccaacaatt ctgaggctgt tttgctccct ctgaatgagc ctgtgcatgg aaccaagagg 840
agaagccaga ttcaaactta cctggagtac cacggtggcc ctggagttca gcatatcgcc 900
cttgcttcta atgatgtgtt gcgcacactc agagagatgc gcgccagaac ccccatggga 960
ggtttcgagt tcatggcccc accacaggct aagtactacg agggcgttag aaggattgct 1020
ggagacgtgt tgagcgagga gcagatcaag gagtgccaag agctgggtgt tcttgtggat 1080
agggatgacc agggcgttct gcttcaaatt gccactaagc cagtgggcga ccgccctaca 1140
ttctggcttg agatgattca aagaatcggc tgcatggaga aggatgaggt tggacaggag 1200
taccaaaagg gcggatgcgg tggcttcgga aagggtaact tcagcgagtt gttcaagtca 1260
atcgaggact acgagaagag cctcgaggtg aagcagtcag tggttgccca aaagtcctga 1320
<210> 176
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-A111T-F372A氨基酸序列(Artificial Sequence)
<400> 176
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Thr Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Phe Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 177
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-A111T-F372A-01核苷酸序列(Artificial Sequence)
<400> 177
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca accaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgtttttcc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 178
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-A111T-F372A-02核苷酸序列(Artificial Sequence)
<400> 178
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct actacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattcttcc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 179
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-A111T-F383W氨基酸序列(Artificial Sequence)
<400> 179
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Thr Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 180
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-A111T-F383W-01核苷酸序列(Artificial Sequence)
<400> 180
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca accaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcttcacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 181
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-A111T-F383W-02核苷酸序列(Artificial Sequence)
<400> 181
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct actacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attttcacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 182
<211> 440
<212> PRT
<213> 人工序列-HPPDm-2氨基酸序列(Artificial Sequence)
<400> 182
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Thr Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 183
<211> 1323
<212> DNA
<213> 人工序列-HPPDm-2-01核苷酸序列(Artificial Sequence)
<400> 183
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca accaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 184
<211> 1323
<212> DNA
<213> 人工序列-HPPDm-2-02核苷酸序列(Artificial Sequence)
<400> 184
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct actacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 185
<211> 440
<212> PRT
<213> 人工序列-HPPDm-3氨基酸序列(Artificial Sequence)
<400> 185
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Gly Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 186
<211> 1323
<212> DNA
<213> 人工序列-HPPDm-3-01核苷酸序列(Artificial Sequence)
<400> 186
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggagggtgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 187
<211> 1323
<212> DNA
<213> 人工序列-HPPDm-3-02核苷酸序列(Artificial Sequence)
<400> 187
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggagggtgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 188
<211> 439
<212> PRT
<213> 人工序列-HPPDm-4氨基酸序列(Artificial Sequence)
<400> 188
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Thr Ala Ala Ala Thr Ala
100 105 110
Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala Ala
115 120 125
His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala Ala
130 135 140
Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe Ala
145 150 155 160
Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu Tyr
165 170 175
Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp Leu
180 185 190
Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val Asp
195 200 205
Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu Met
210 215 220
Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu Phe
225 230 235 240
Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu Asn
245 250 255
Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu Asn
260 265 270
Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr Leu
275 280 285
Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser Asn
290 295 300
Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met Gly
305 310 315 320
Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly Val
325 330 335
Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Asn Glu Cys
340 345 350
Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu Leu
355 360 365
Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu Glu
370 375 380
Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln Glu
385 390 395 400
Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu
405 410 415
Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys Gln
420 425 430
Ser Val Val Ala Gln Lys Ser
435
<210> 189
<211> 1320
<212> DNA
<213> 人工序列-HPPDm-4-01核苷酸序列(Artificial Sequence)
<400> 189
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccac ggccgcagcc accgcctcca tcccctcctt ctccgccgac 360
gccgcgcgga cgttcgccgc cgcccacggc ctcgcggtgc gctccgtcgg ggtccgcgtc 420
gctgacgccg ccgaggcctt ccgcgtcagc gtagccggcg gcgctcgccc ggccttcgcc 480
ccagccgacc tcggccatgg cttcggcctc gccgaggtcg agctctacgg cgacgtcgtg 540
ctacgcttcg tcagctaccc ggacgagaca gacctgccat tcctgccagg gttcgagcgc 600
gtgagcagcc ccggcgccgt ggactacggc ctcacgcggt tcgaccacgt cgtgggcaac 660
gtcccggaga tggccccggt catagactac atgaaaggct tcttggggtt ccacgagttc 720
gccgagttca ccgccgagga cgtgggcacg accgagagcg ggctcaactc ggtggtgctc 780
gccaacaact ccgaggccgt gctgctgccg ctcaacgagc ccgtgcacgg cacaaagcga 840
cggagccaga tacagacgta cctggagtat cacggcgggc ccggcgtgca gcacatcgcg 900
ctcgccagca acgacgtgct caggacgctc agggagatgc gggcgcgcac gcccatgggc 960
ggcttcgagt tcatggcgcc accgcaggcg aaatactatg aaggcgtgcg gcgcatcgca 1020
ggtgacgtgc tctcggaaga gcagatcaat gaatgccagg agctgggggt gctagtcgac 1080
agggatgatc aaggggtgtt gctccaaatc gctaccaagc cagtagggga caggccaacg 1140
ttttggctgg agatgatcca aagaatcggg tgcatggaga aggacgaggt cgggcaagag 1200
taccagaagg gtggctgcgg cgggtttggc aagggcaatt tctccgagct gttcaagtcc 1260
attgaggact atgagaaatc ccttgaggtc aagcaatctg ttgtagctca gaaatcctag 1320
<210> 190
<211> 1320
<212> DNA
<213> 人工序列-HPPDm-4-02核苷酸序列(Artificial Sequence)
<400> 190
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctac tgctgctgct acagcctcca ttccaagctt ctcagctgat 360
gccgctagaa ccttcgctgc tgctcatgga cttgccgtga ggtctgttgg tgttcgcgtg 420
gctgatgctg ctgaggcttt cagagtttcc gttgccggcg gagctaggcc agctttcgct 480
cctgctgatt tgggacatgg tttcggcctg gctgaggttg agctttacgg tgacgtggtt 540
ctccgcttcg tgtcttaccc agatgagact gacctgcctt tccttcccgg cttcgagaga 600
gtttcctctc ctggtgccgt ggattacggc ctgacaaggt tcgaccacgt ggttggaaac 660
gttcccgaga tggctccagt gatcgattac atgaagggct tccttggatt ccatgagttc 720
gccgagttca ctgctgagga cgtgggaacc actgagtccg gtttgaactc tgtggttctc 780
gccaacaatt ctgaggctgt tttgctccct ctgaatgagc ctgtgcatgg aaccaagagg 840
agaagccaga ttcaaactta cctggagtac cacggtggcc ctggagttca gcatatcgcc 900
cttgcttcta atgatgtgtt gcgcacactc agagagatgc gcgccagaac ccccatggga 960
ggtttcgagt tcatggcccc accacaggct aagtactacg agggcgttag aaggattgct 1020
ggagacgtgt tgagcgagga gcagatcaat gagtgccaag agctgggtgt tcttgtggat 1080
agggatgacc agggcgttct gcttcaaatt gccactaagc cagtgggcga ccgccctaca 1140
ttctggcttg agatgattca aagaatcggc tgcatggaga aggatgaggt tggacaggag 1200
taccaaaagg gcggatgcgg tggcttcgga aagggtaact tcagcgagtt gttcaagtca 1260
atcgaggact acgagaagag cctcgaggtg aagcagtcag tggttgccca aaagtcctga 1320
<210> 191
<211> 440
<212> PRT
<213> 人工序列-HPPDm-5氨基酸序列(Artificial Sequence)
<400> 191
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Thr Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Asn Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 192
<211> 1323
<212> DNA
<213> 人工序列-HPPDm-5-01核苷酸序列(Artificial Sequence)
<400> 192
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca accaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aatgaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 193
<211> 1323
<212> DNA
<213> 人工序列-HPPDm-5-02核苷酸序列(Artificial Sequence)
<400> 193
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct actacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aatgagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 194
<211> 440
<212> PRT
<213> 人工序列-HPPDm-6氨基酸序列(Artificial Sequence)
<400> 194
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Gly Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Asn Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 195
<211> 1323
<212> DNA
<213> 人工序列-HPPDm-6-01核苷酸序列(Artificial Sequence)
<400> 195
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggagggtgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aatgaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 196
<211> 1323
<212> DNA
<213> 人工序列-HPPDm-6-02核苷酸序列(Artificial Sequence)
<400> 196
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggagggtgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aatgagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 197
<211> 435
<212> PRT
<213> 人工序列-HPPDm-7的氨基酸序列(Artificial Sequence)
<400> 197
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Thr Ala Ala Ala Thr Ala
100 105 110
Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala Ala
115 120 125
His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala Ala
130 135 140
Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe Ala
145 150 155 160
Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu Tyr
165 170 175
Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp Leu
180 185 190
Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val Asp
195 200 205
Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu Met
210 215 220
Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu Phe
225 230 235 240
Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu Asn
245 250 255
Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu Asn
260 265 270
Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr Leu
275 280 285
Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser Asn
290 295 300
Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met Gly
305 310 315 320
Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly Val
325 330 335
Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Asn Glu Cys
340 345 350
Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu Leu
355 360 365
Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu Glu
370 375 380
Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln Glu
385 390 395 400
Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu
405 410 415
Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr Leu Glu Ala Lys Gln
420 425 430
Leu Val Gly
435
<210> 198
<211> 1308
<212> DNA
<213> 人工序列-HPPDm-7-01核苷酸序列(Artificial Sequence)
<400> 198
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccac ggccgcagcc accgcctcca tcccctcctt ctccgccgac 360
gccgcgcgga cgttcgccgc cgcccacggc ctcgcggtgc gctccgtcgg ggtccgcgtc 420
gctgacgccg ccgaggcctt ccgcgtcagc gtagccggcg gcgctcgccc ggccttcgcc 480
ccagccgacc tcggccatgg cttcggcctc gccgaggtcg agctctacgg cgacgtcgtg 540
ctacgcttcg tcagctaccc ggacgagaca gacctgccat tcctgccagg gttcgagcgc 600
gtgagcagcc ccggcgccgt ggactacggc ctcacgcggt tcgaccacgt cgtgggcaac 660
gtcccggaga tggccccggt catagactac atgaaaggct tcttggggtt ccacgagttc 720
gccgagttca ccgccgagga cgtgggcacg accgagagcg ggctcaactc ggtggtgctc 780
gccaacaact ccgaggccgt gctgctgccg ctcaacgagc ccgtgcacgg cacaaagcga 840
cggagccaga tacagacgta cctggagtat cacggcgggc ccggcgtgca gcacatcgcg 900
ctcgccagca acgacgtgct caggacgctc agggagatgc gggcgcgcac gcccatgggc 960
ggcttcgagt tcatggcgcc accgcaggcg aaatactatg aaggcgtgcg gcgcatcgca 1020
ggtgacgtgc tctcggaaga gcagatcaat gaatgccagg agctgggggt gctagtcgac 1080
agggatgatc aaggggtgtt gctccaaatc gctaccaagc cagtagggga caggccaacg 1140
ttttggctgg agatgatcca aagaatcggg tgcatggaga aggacgaggt cgggcaagag 1200
taccagaagg gtggctgcgg cgggtttggc aagggcaatt tctccgagct gttcaagtcc 1260
attgaggaat acgaaaagac tcttgaagcc aaacagttag tgggatga 1308
<210> 199
<211> 1308
<212> DNA
<213> 人工序列-HPPDm-7-02核苷酸序列(Artificial Sequence)
<400> 199
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctac tgctgctgct acagcctcca ttccaagctt ctcagctgat 360
gccgctagaa ccttcgctgc tgctcatgga cttgccgtga ggtctgttgg tgttcgcgtg 420
gctgatgctg ctgaggcttt cagagtttcc gttgccggcg gagctaggcc agctttcgct 480
cctgctgatt tgggacatgg tttcggcctg gctgaggttg agctttacgg tgacgtggtt 540
ctccgcttcg tgtcttaccc agatgagact gacctgcctt tccttcccgg cttcgagaga 600
gtttcctctc ctggtgccgt ggattacggc ctgacaaggt tcgaccacgt ggttggaaac 660
gttcccgaga tggctccagt gatcgattac atgaagggct tccttggatt ccatgagttc 720
gccgagttca ctgctgagga cgtgggaacc actgagtccg gtttgaactc tgtggttctc 780
gccaacaatt ctgaggctgt tttgctccct ctgaatgagc ctgtgcatgg aaccaagagg 840
agaagccaga ttcaaactta cctggagtac cacggtggcc ctggagttca gcatatcgcc 900
cttgcttcta atgatgtgtt gcgcacactc agagagatgc gcgccagaac ccccatggga 960
ggtttcgagt tcatggcccc accacaggct aagtactacg agggcgttag aaggattgct 1020
ggagacgtgt tgagcgagga gcagatcaat gagtgccaag agctgggtgt tcttgtggat 1080
agggatgacc agggcgttct gcttcaaatt gccactaagc cagtgggcga ccgccctaca 1140
ttctggcttg agatgattca aagaatcggc tgcatggaga aggatgaggt tggacaggag 1200
taccaaaagg gcggatgcgg tggcttcgga aagggtaact tcagcgagtt gttcaagtca 1260
atcgaggagt atgagaagac cttggaggcc aagcagctcg tgggttga 1308
<210> 200
<211> 436
<212> PRT
<213> 人工序列-HPPDm-8的氨基酸序列(Artificial Sequence)
<400> 200
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Thr Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Asn Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr Leu Glu Ala Lys
420 425 430
Gln Leu Val Gly
435
<210> 201
<211> 1311
<212> DNA
<213> 人工序列-HPPDm-8-01核苷酸序列(Artificial Sequence)
<400> 201
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca accaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aatgaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg aatacgaaaa gactcttgaa gccaaacagt tagtgggatg a 1311
<210> 202
<211> 1311
<212> DNA
<213> 人工序列-HPPDm-8-02核苷酸序列(Artificial Sequence)
<400> 202
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct actacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aatgagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg agtatgagaa gaccttggag gccaagcagc tcgtgggttg a 1311
<210> 203
<211> 436
<212> PRT
<213> 人工序列-HPPDm-9的氨基酸序列(Artificial Sequence)
<400> 203
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Gly Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Asn Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Trp Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Glu Tyr Glu Lys Thr Leu Glu Ala Lys
420 425 430
Gln Leu Val Gly
435
<210> 204
<211> 1311
<212> DNA
<213> 人工序列-HPPDm-9-01核苷酸序列(Artificial Sequence)
<400> 204
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggagggtgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aatgaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgttttggc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg aatacgaaaa gactcttgaa gccaaacagt tagtgggatg a 1311
<210> 205
<211> 1311
<212> DNA
<213> 人工序列-HPPDm-9-02核苷酸序列(Artificial Sequence)
<400> 205
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggagggtgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aatgagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattctggc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg agtatgagaa gaccttggag gccaagcagc tcgtgggttg a 1311
<210> 206
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F415W氨基酸序列(Artificial Sequence)
<400> 206
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Phe Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Trp Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 207
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F415W-01核苷酸序列(Artificial Sequence)
<400> 207
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcttcacca agccagtagg ggacaggcca 1140
acgtttttcc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca attggtccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 208
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F415W-02核苷酸序列(Artificial Sequence)
<400> 208
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attttcacta agccagtggg cgaccgccct 1140
acattcttcc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta actggagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 209
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F372A-F415W氨基酸序列(Artificial Sequence)
<400> 209
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Phe Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Trp Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 210
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372A-F415W-01核苷酸序列(Artificial Sequence)
<400> 210
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgtttttcc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca attggtccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 211
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372A-F415W-02核苷酸序列(Artificial Sequence)
<400> 211
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattcttcc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta actggagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 212
<211> 445
<212> PRT
<213> 人工序列-AtHPPDm-F415W氨基酸序列(Artificial Sequence)
<400> 212
Met Gly His Gln Asn Ala Ala Val Ser Glu Asn Gln Asn His Asp Asp
1 5 10 15
Gly Ala Ala Ser Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe
20 25 30
Val Arg Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His
35 40 45
His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ala Arg Arg Phe
50 55 60
Ser Trp Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr
65 70 75 80
Gly Asn Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg
85 90 95
Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ile
100 105 110
Lys Pro Thr Thr Thr Ala Ser Ile Pro Ser Phe Asp His Gly Ser Cys
115 120 125
Arg Ser Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile
130 135 140
Glu Val Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly
145 150 155 160
Ala Ile Pro Ser Ser Pro Pro Ile Val Leu Asn Glu Ala Val Thr Ile
165 170 175
Ala Glu Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr
180 185 190
Lys Ala Glu Asp Thr Glu Lys Ser Glu Phe Leu Pro Gly Phe Glu Arg
195 200 205
Val Glu Asp Ala Ser Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu
210 215 220
Asp His Ala Val Gly Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr
225 230 235 240
Val Ala Gly Phe Thr Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp
245 250 255
Asp Val Gly Thr Ala Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Ser
260 265 270
Asn Asp Glu Met Val Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr
275 280 285
Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala
290 295 300
Gly Leu Gln His Leu Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu
305 310 315 320
Arg Glu Met Arg Lys Arg Ser Ser Ile Gly Gly Phe Asp Phe Met Pro
325 330 335
Ser Pro Pro Pro Thr Tyr Tyr Gln Asn Leu Lys Lys Arg Val Gly Asp
340 345 350
Val Leu Ser Asp Asp Gln Ile Lys Glu Cys Glu Glu Leu Gly Ile Leu
355 360 365
Val Asp Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro
370 375 380
Leu Gly Asp Arg Pro Thr Ile Phe Ile Glu Ile Ile Gln Arg Val Gly
385 390 395 400
Cys Met Met Lys Asp Glu Glu Gly Lys Ala Tyr Gln Ser Gly Gly Cys
405 410 415
Gly Gly Phe Gly Lys Gly Asn Trp Ser Glu Leu Phe Lys Ser Ile Glu
420 425 430
Glu Tyr Glu Lys Thr Leu Glu Ala Lys Gln Leu Val Gly
435 440 445
<210> 213
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F415W-01核苷酸序列(Artificial Sequence)
<400> 213
atgggccacc aaaacgccgc cgtttcagag aatcaaaacc atgatgacgg cgctgcgtcg 60
tcgccgggat tcaagctcgt cggattttcc aagttcgtaa gaaagaatcc aaagtctgat 120
aaattcaagg ttaagcgctt ccatcacatc gagttctggt gcggcgacgc aaccaacgtc 180
gctcgtcgct tctcctgggg tctggggatg agattctccg ccaaatccga tctttccacc 240
ggaaacatgg ttcacgcctc ttacctactc acctccggtg acctccgatt ccttttcact 300
gctccttact ctccgtctct ctccgccgga gagattaaac cgacaaccac agcttctatc 360
ccaagtttcg atcacggctc ttgtcgttcc ttcttctctt cacatggtct cggtgttaga 420
gccgttgcga ttgaagtaga agacgcagag tcagctttct ccatcagtgt agctaatggc 480
gctattcctt cgtcgcctcc tatcgtcctc aatgaagcag ttacgatcgc tgaggttaaa 540
ctatacggcg atgttgttct ccgatatgtt agttacaaag cagaagatac cgaaaaatcc 600
gaattcttgc cagggttcga gcgtgtagag gatgcgtcgt cgttcccatt ggattatggt 660
atccggcggc ttgaccacgc cgtgggaaac gttcctgagc ttggtccggc tttaacttat 720
gtagcggggt tcactggttt tcaccaattc gcagagttca cagcagacga cgttggaacc 780
gccgagagcg gtttaaattc agcggtcctg gctagcaatg atgaaatggt tcttctaccg 840
attaacgagc cagtgcacgg aacaaagagg aagagtcaga ttcagacgta tttggaacat 900
aacgaaggcg cagggctaca acatctggct ctgatgagtg aagacatatt caggaccctg 960
agagagatga ggaagaggag cagtattgga ggattcgact tcatgccttc tcctccgcct 1020
acttactacc agaatctcaa gaaacgggtc ggcgacgtgc tcagcgatga tcagatcaag 1080
gagtgtgagg aattagggat tcttgtagac agagatgatc aagggacgtt gcttcaaatc 1140
ttcacaaaac cactaggtga caggccgacg atatttatag agataatcca gagagtagga 1200
tgcatgatga aagatgagga agggaaggct taccagagtg gaggatgtgg tggttttggc 1260
aaaggcaatt ggtctgagct cttcaagtcc attgaagaat acgaaaagac tcttgaagcc 1320
aaacagttag tgggatga 1338
<210> 214
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F415W-02核苷酸序列(Artificial Sequence)
<400> 214
atgggacatc agaatgctgc tgtatcagaa aatcaaaatc atgatgatgg agccgcctcc 60
tcaccagggt ttaagttggt tgggttctcc aagtttgtac ggaagaaccc aaaatccgac 120
aagttcaagg ttaaacgctt ccaccacatt gaattctggt gcggcgacgc caccaatgtt 180
gctcggcgtt tttcatgggg tttaggcatg agattctcag caaagagtga cctgagcacc 240
ggcaacatgg ttcatgctag ctatctctta acatctggag atttgaggtt tcttttcacg 300
gcgccgtact caccatcact ctccgccggc gagatcaaac ccaccacgac tgcaagcata 360
ccttcatttg atcatggtag ttgcagatcc ttcttctcgt cgcacggcct aggggtgcgc 420
gcggtcgcca tcgaagttga agatgcagaa tcagcgttta gtatcagcgt ggcaaatgga 480
gctattcctt cctcccctcc aattgtcctc aatgaggctg taactattgc cgaggtgaag 540
ttgtatggag atgttgtgct gagatatgtt tcttacaagg cagaggacac tgagaaatct 600
gaatttttgc ctggttttga aagggtcgag gatgcttcaa gttttcctct tgattacggc 660
atacgacggt tggatcacgc ggttggaaat gtaccagaac ttgggccggc gctcacttat 720
gtggctggtt tcactggatt ccatcaattt gctgagttca cagctgatga tgttgggact 780
gctgaaagtg ggttgaactc tgcagtcctc gcctctaatg atgaaatggt gctcctcccg 840
atcaacgagc ctgtgcatgg caccaaaagg aaatcacaaa tacagacata tcttgagcac 900
aatgaaggtg cagggcttca acatcttgca ctgatgtctg aagacatttt ccgtacgctg 960
agagagatga ggaagagaag tagtattggt ggatttgact tcatgccttc tcccccacca 1020
acttactacc agaacttgaa gaagagggtt ggtgatgtgc tatctgatga ccagatcaag 1080
gagtgtgaag aattgggcat tcttgttgat cgggacgacc aaggaacatt actgcagata 1140
tttacaaaac cacttggtga ccgccccact atttttattg agatcataca aagagtcggc 1200
tgcatgatga aggatgagga aggaaaagca tatcaatctg gtggttgtgg cggctttggg 1260
aaaggaaact ggtctgagct gttcaaatcc atcgaggagt atgagaagac cttggaggcc 1320
aagcagctcg tgggttga 1338
<210> 215
<211> 445
<212> PRT
<213> 人工序列-AtHPPDm-F372A-F415W氨基酸序列(Artificial Sequence)
<400> 215
Met Gly His Gln Asn Ala Ala Val Ser Glu Asn Gln Asn His Asp Asp
1 5 10 15
Gly Ala Ala Ser Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe
20 25 30
Val Arg Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His
35 40 45
His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ala Arg Arg Phe
50 55 60
Ser Trp Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr
65 70 75 80
Gly Asn Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg
85 90 95
Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ile
100 105 110
Lys Pro Thr Thr Thr Ala Ser Ile Pro Ser Phe Asp His Gly Ser Cys
115 120 125
Arg Ser Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile
130 135 140
Glu Val Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly
145 150 155 160
Ala Ile Pro Ser Ser Pro Pro Ile Val Leu Asn Glu Ala Val Thr Ile
165 170 175
Ala Glu Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr
180 185 190
Lys Ala Glu Asp Thr Glu Lys Ser Glu Phe Leu Pro Gly Phe Glu Arg
195 200 205
Val Glu Asp Ala Ser Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu
210 215 220
Asp His Ala Val Gly Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr
225 230 235 240
Val Ala Gly Phe Thr Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp
245 250 255
Asp Val Gly Thr Ala Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Ser
260 265 270
Asn Asp Glu Met Val Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr
275 280 285
Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala
290 295 300
Gly Leu Gln His Leu Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu
305 310 315 320
Arg Glu Met Arg Lys Arg Ser Ser Ile Gly Gly Phe Asp Phe Met Pro
325 330 335
Ser Pro Pro Pro Thr Tyr Tyr Gln Asn Leu Lys Lys Arg Val Gly Asp
340 345 350
Val Leu Ser Asp Asp Gln Ile Lys Glu Cys Glu Glu Leu Gly Ile Leu
355 360 365
Val Asp Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro
370 375 380
Leu Gly Asp Arg Pro Thr Ile Phe Ile Glu Ile Ile Gln Arg Val Gly
385 390 395 400
Cys Met Met Lys Asp Glu Glu Gly Lys Ala Tyr Gln Ser Gly Gly Cys
405 410 415
Gly Gly Phe Gly Lys Gly Asn Trp Ser Glu Leu Phe Lys Ser Ile Glu
420 425 430
Glu Tyr Glu Lys Thr Leu Glu Ala Lys Gln Leu Val Gly
435 440 445
<210> 216
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F372A-F415W-01核苷酸序列(Artificial Sequence)
<400> 216
atgggccacc aaaacgccgc cgtttcagag aatcaaaacc atgatgacgg cgctgcgtcg 60
tcgccgggat tcaagctcgt cggattttcc aagttcgtaa gaaagaatcc aaagtctgat 120
aaattcaagg ttaagcgctt ccatcacatc gagttctggt gcggcgacgc aaccaacgtc 180
gctcgtcgct tctcctgggg tctggggatg agattctccg ccaaatccga tctttccacc 240
ggaaacatgg ttcacgcctc ttacctactc acctccggtg acctccgatt ccttttcact 300
gctccttact ctccgtctct ctccgccgga gagattaaac cgacaaccac agcttctatc 360
ccaagtttcg atcacggctc ttgtcgttcc ttcttctctt cacatggtct cggtgttaga 420
gccgttgcga ttgaagtaga agacgcagag tcagctttct ccatcagtgt agctaatggc 480
gctattcctt cgtcgcctcc tatcgtcctc aatgaagcag ttacgatcgc tgaggttaaa 540
ctatacggcg atgttgttct ccgatatgtt agttacaaag cagaagatac cgaaaaatcc 600
gaattcttgc cagggttcga gcgtgtagag gatgcgtcgt cgttcccatt ggattatggt 660
atccggcggc ttgaccacgc cgtgggaaac gttcctgagc ttggtccggc tttaacttat 720
gtagcggggt tcactggttt tcaccaattc gcagagttca cagcagacga cgttggaacc 780
gccgagagcg gtttaaattc agcggtcctg gctagcaatg atgaaatggt tcttctaccg 840
attaacgagc cagtgcacgg aacaaagagg aagagtcaga ttcagacgta tttggaacat 900
aacgaaggcg cagggctaca acatctggct ctgatgagtg aagacatatt caggaccctg 960
agagagatga ggaagaggag cagtattgga ggattcgact tcatgccttc tcctccgcct 1020
acttactacc agaatctcaa gaaacgggtc ggcgacgtgc tcagcgatga tcagatcaag 1080
gagtgtgagg aattagggat tcttgtagac agagatgatc aagggacgtt gcttcaaatc 1140
gctacaaaac cactaggtga caggccgacg atatttatag agataatcca gagagtagga 1200
tgcatgatga aagatgagga agggaaggct taccagagtg gaggatgtgg tggttttggc 1260
aaaggcaatt ggtctgagct cttcaagtcc attgaagaat acgaaaagac tcttgaagcc 1320
aaacagttag tgggatga 1338
<210> 217
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F372A-F415W-02核苷酸序列(Artificial Sequence)
<400> 217
atgggacatc agaatgctgc tgtatcagaa aatcaaaatc atgatgatgg agccgcctcc 60
tcaccagggt ttaagttggt tgggttctcc aagtttgtac ggaagaaccc aaaatccgac 120
aagttcaagg ttaaacgctt ccaccacatt gaattctggt gcggcgacgc caccaatgtt 180
gctcggcgtt tttcatgggg tttaggcatg agattctcag caaagagtga cctgagcacc 240
ggcaacatgg ttcatgctag ctatctctta acatctggag atttgaggtt tcttttcacg 300
gcgccgtact caccatcact ctccgccggc gagatcaaac ccaccacgac tgcaagcata 360
ccttcatttg atcatggtag ttgcagatcc ttcttctcgt cgcacggcct aggggtgcgc 420
gcggtcgcca tcgaagttga agatgcagaa tcagcgttta gtatcagcgt ggcaaatgga 480
gctattcctt cctcccctcc aattgtcctc aatgaggctg taactattgc cgaggtgaag 540
ttgtatggag atgttgtgct gagatatgtt tcttacaagg cagaggacac tgagaaatct 600
gaatttttgc ctggttttga aagggtcgag gatgcttcaa gttttcctct tgattacggc 660
atacgacggt tggatcacgc ggttggaaat gtaccagaac ttgggccggc gctcacttat 720
gtggctggtt tcactggatt ccatcaattt gctgagttca cagctgatga tgttgggact 780
gctgaaagtg ggttgaactc tgcagtcctc gcctctaatg atgaaatggt gctcctcccg 840
atcaacgagc ctgtgcatgg caccaaaagg aaatcacaaa tacagacata tcttgagcac 900
aatgaaggtg cagggcttca acatcttgca ctgatgtctg aagacatttt ccgtacgctg 960
agagagatga ggaagagaag tagtattggt ggatttgact tcatgccttc tcccccacca 1020
acttactacc agaacttgaa gaagagggtt ggtgatgtgc tatctgatga ccagatcaag 1080
gagtgtgaag aattgggcat tcttgttgat cgggacgacc aaggaacatt actgcagata 1140
gccacaaaac cacttggtga ccgccccact atttttattg agatcataca aagagtcggc 1200
tgcatgatga aggatgagga aggaaaagca tatcaatctg gtggttgtgg cggctttggg 1260
aaaggaaact ggtctgagct gttcaaatcc atcgaggagt atgagaagac cttggaggcc 1320
aagcagctcg tgggttga 1338
<210> 218
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F383Y氨基酸序列(Artificial Sequence)
<400> 218
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Phe Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Tyr Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 219
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F383Y-01核苷酸序列(Artificial Sequence)
<400> 219
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcttcacca agccagtagg ggacaggcca 1140
acgttttatc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 220
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F383Y-02核苷酸序列(Artificial Sequence)
<400> 220
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attttcacta agccagtggg cgaccgccct 1140
acattctatc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 221
<211> 440
<212> PRT
<213> 人工序列-AsHPPDm-F372A-F383Y氨基酸序列(Artificial Sequence)
<400> 221
Met Pro Pro Thr Pro Ala Thr Ala Thr Gly Ala Ala Ala Ala Ala Val
1 5 10 15
Thr Pro Glu His Ala Ala Arg Ser Phe Pro Arg Val Val Arg Val Asn
20 25 30
Pro Arg Ser Asp Arg Phe Pro Val Leu Ser Phe His His Val Glu Leu
35 40 45
Trp Cys Ala Asp Ala Ala Ser Ala Ala Gly Arg Phe Ser Phe Ala Leu
50 55 60
Gly Ala Pro Leu Ala Ala Arg Ser Asp Leu Ser Thr Gly Asn Ser Ala
65 70 75 80
His Ala Ser Leu Leu Leu Arg Ser Gly Ala Leu Ala Phe Leu Phe Thr
85 90 95
Ala Pro Tyr Ala Pro Pro Pro Gln Glu Ala Ala Thr Ala Ala Ala Thr
100 105 110
Ala Ser Ile Pro Ser Phe Ser Ala Asp Ala Ala Arg Thr Phe Ala Ala
115 120 125
Ala His Gly Leu Ala Val Arg Ser Val Gly Val Arg Val Ala Asp Ala
130 135 140
Ala Glu Ala Phe Arg Val Ser Val Ala Gly Gly Ala Arg Pro Ala Phe
145 150 155 160
Ala Pro Ala Asp Leu Gly His Gly Phe Gly Leu Ala Glu Val Glu Leu
165 170 175
Tyr Gly Asp Val Val Leu Arg Phe Val Ser Tyr Pro Asp Glu Thr Asp
180 185 190
Leu Pro Phe Leu Pro Gly Phe Glu Arg Val Ser Ser Pro Gly Ala Val
195 200 205
Asp Tyr Gly Leu Thr Arg Phe Asp His Val Val Gly Asn Val Pro Glu
210 215 220
Met Ala Pro Val Ile Asp Tyr Met Lys Gly Phe Leu Gly Phe His Glu
225 230 235 240
Phe Ala Glu Phe Thr Ala Glu Asp Val Gly Thr Thr Glu Ser Gly Leu
245 250 255
Asn Ser Val Val Leu Ala Asn Asn Ser Glu Ala Val Leu Leu Pro Leu
260 265 270
Asn Glu Pro Val His Gly Thr Lys Arg Arg Ser Gln Ile Gln Thr Tyr
275 280 285
Leu Glu Tyr His Gly Gly Pro Gly Val Gln His Ile Ala Leu Ala Ser
290 295 300
Asn Asp Val Leu Arg Thr Leu Arg Glu Met Arg Ala Arg Thr Pro Met
305 310 315 320
Gly Gly Phe Glu Phe Met Ala Pro Pro Gln Ala Lys Tyr Tyr Glu Gly
325 330 335
Val Arg Arg Ile Ala Gly Asp Val Leu Ser Glu Glu Gln Ile Lys Glu
340 345 350
Cys Gln Glu Leu Gly Val Leu Val Asp Arg Asp Asp Gln Gly Val Leu
355 360 365
Leu Gln Ile Ala Thr Lys Pro Val Gly Asp Arg Pro Thr Phe Tyr Leu
370 375 380
Glu Met Ile Gln Arg Ile Gly Cys Met Glu Lys Asp Glu Val Gly Gln
385 390 395 400
Glu Tyr Gln Lys Gly Gly Cys Gly Gly Phe Gly Lys Gly Asn Phe Ser
405 410 415
Glu Leu Phe Lys Ser Ile Glu Asp Tyr Glu Lys Ser Leu Glu Val Lys
420 425 430
Gln Ser Val Val Ala Gln Lys Ser
435 440
<210> 222
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372A-F383Y-01核苷酸序列(Artificial Sequence)
<400> 222
atgccgccca cccccgccac cgccaccggc gccgccgcgg ccgccgtgac tccagagcac 60
gcggcccgga gctttccccg agtggtccgc gtcaacccgc gcagcgaccg cttccccgtg 120
ctctccttcc accacgtcga gctctggtgc gccgacgccg cctcagcggc cggacgcttc 180
tccttcgcgc tcggcgcgcc gctcgccgcc cggtccgacc tctccacggg gaactccgcg 240
cacgcctccc tcctgctccg ctcgggcgcc ctcgccttcc tcttcacggc gccctacgcg 300
ccgccgccgc aggaggccgc cacggccgca gccaccgcct ccatcccctc cttctccgcc 360
gacgccgcgc ggacgttcgc cgccgcccac ggcctcgcgg tgcgctccgt cggggtccgc 420
gtcgctgacg ccgccgaggc cttccgcgtc agcgtagccg gcggcgctcg cccggccttc 480
gccccagccg acctcggcca tggcttcggc ctcgccgagg tcgagctcta cggcgacgtc 540
gtgctacgct tcgtcagcta cccggacgag acagacctgc cattcctgcc agggttcgag 600
cgcgtgagca gccccggcgc cgtggactac ggcctcacgc ggttcgacca cgtcgtgggc 660
aacgtcccgg agatggcccc ggtcatagac tacatgaaag gcttcttggg gttccacgag 720
ttcgccgagt tcaccgccga ggacgtgggc acgaccgaga gcgggctcaa ctcggtggtg 780
ctcgccaaca actccgaggc cgtgctgctg ccgctcaacg agcccgtgca cggcacaaag 840
cgacggagcc agatacagac gtacctggag tatcacggcg ggcccggcgt gcagcacatc 900
gcgctcgcca gcaacgacgt gctcaggacg ctcagggaga tgcgggcgcg cacgcccatg 960
ggcggcttcg agttcatggc gccaccgcag gcgaaatact atgaaggcgt gcggcgcatc 1020
gcaggtgacg tgctctcgga agagcagatc aaggaatgcc aggagctggg ggtgctagtc 1080
gacagggatg atcaaggggt gttgctccaa atcgctacca agccagtagg ggacaggcca 1140
acgttttatc tggagatgat ccaaagaatc gggtgcatgg agaaggacga ggtcgggcaa 1200
gagtaccaga agggtggctg cggcgggttt ggcaagggca atttctccga gctgttcaag 1260
tccattgagg actatgagaa atcccttgag gtcaagcaat ctgttgtagc tcagaaatcc 1320
tag 1323
<210> 223
<211> 1323
<212> DNA
<213> 人工序列-AsHPPDm-F372A-F383Y-02核苷酸序列(Artificial Sequence)
<400> 223
atgccaccta ctccagctac agctactggt gctgctgctg ctgccgttac tcctgagcac 60
gctgccaggt cattccccag ggtggttcgc gtgaacccca gatctgatag gttcccagtt 120
ctgagcttcc accatgttga gctgtggtgc gctgatgctg cttctgctgc tggcaggttc 180
tctttcgctc ttggtgctcc actcgctgct agatctgatc tgtctacagg aaatagcgct 240
catgcctcac tcctgcttcg ctcaggtgct ttggccttcc tcttcaccgc tccatatgct 300
cccccacctc aggaggctgc tactgctgct gctacagcct ccattccaag cttctcagct 360
gatgccgcta gaaccttcgc tgctgctcat ggacttgccg tgaggtctgt tggtgttcgc 420
gtggctgatg ctgctgaggc tttcagagtt tccgttgccg gcggagctag gccagctttc 480
gctcctgctg atttgggaca tggtttcggc ctggctgagg ttgagcttta cggtgacgtg 540
gttctccgct tcgtgtctta cccagatgag actgacctgc ctttccttcc cggcttcgag 600
agagtttcct ctcctggtgc cgtggattac ggcctgacaa ggttcgacca cgtggttgga 660
aacgttcccg agatggctcc agtgatcgat tacatgaagg gcttccttgg attccatgag 720
ttcgccgagt tcactgctga ggacgtggga accactgagt ccggtttgaa ctctgtggtt 780
ctcgccaaca attctgaggc tgttttgctc cctctgaatg agcctgtgca tggaaccaag 840
aggagaagcc agattcaaac ttacctggag taccacggtg gccctggagt tcagcatatc 900
gcccttgctt ctaatgatgt gttgcgcaca ctcagagaga tgcgcgccag aacccccatg 960
ggaggtttcg agttcatggc cccaccacag gctaagtact acgagggcgt tagaaggatt 1020
gctggagacg tgttgagcga ggagcagatc aaggagtgcc aagagctggg tgttcttgtg 1080
gatagggatg accagggcgt tctgcttcaa attgccacta agccagtggg cgaccgccct 1140
acattctatc ttgagatgat tcaaagaatc ggctgcatgg agaaggatga ggttggacag 1200
gagtaccaaa agggcggatg cggtggcttc ggaaagggta acttcagcga gttgttcaag 1260
tcaatcgagg actacgagaa gagcctcgag gtgaagcagt cagtggttgc ccaaaagtcc 1320
tga 1323
<210> 224
<211> 445
<212> PRT
<213> 人工序列-AtHPPDm-F383Y氨基酸序列(Artificial Sequence)
<400> 224
Met Gly His Gln Asn Ala Ala Val Ser Glu Asn Gln Asn His Asp Asp
1 5 10 15
Gly Ala Ala Ser Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe
20 25 30
Val Arg Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His
35 40 45
His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ala Arg Arg Phe
50 55 60
Ser Trp Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr
65 70 75 80
Gly Asn Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg
85 90 95
Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ile
100 105 110
Lys Pro Thr Thr Thr Ala Ser Ile Pro Ser Phe Asp His Gly Ser Cys
115 120 125
Arg Ser Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile
130 135 140
Glu Val Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly
145 150 155 160
Ala Ile Pro Ser Ser Pro Pro Ile Val Leu Asn Glu Ala Val Thr Ile
165 170 175
Ala Glu Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr
180 185 190
Lys Ala Glu Asp Thr Glu Lys Ser Glu Phe Leu Pro Gly Phe Glu Arg
195 200 205
Val Glu Asp Ala Ser Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu
210 215 220
Asp His Ala Val Gly Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr
225 230 235 240
Val Ala Gly Phe Thr Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp
245 250 255
Asp Val Gly Thr Ala Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Ser
260 265 270
Asn Asp Glu Met Val Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr
275 280 285
Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala
290 295 300
Gly Leu Gln His Leu Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu
305 310 315 320
Arg Glu Met Arg Lys Arg Ser Ser Ile Gly Gly Phe Asp Phe Met Pro
325 330 335
Ser Pro Pro Pro Thr Tyr Tyr Gln Asn Leu Lys Lys Arg Val Gly Asp
340 345 350
Val Leu Ser Asp Asp Gln Ile Lys Glu Cys Glu Glu Leu Gly Ile Leu
355 360 365
Val Asp Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Phe Thr Lys Pro
370 375 380
Leu Gly Asp Arg Pro Thr Ile Tyr Ile Glu Ile Ile Gln Arg Val Gly
385 390 395 400
Cys Met Met Lys Asp Glu Glu Gly Lys Ala Tyr Gln Ser Gly Gly Cys
405 410 415
Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu
420 425 430
Glu Tyr Glu Lys Thr Leu Glu Ala Lys Gln Leu Val Gly
435 440 445
<210> 225
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F383Y-01核苷酸序列(Artificial Sequence)
<400> 225
atgggccacc aaaacgccgc cgtttcagag aatcaaaacc atgatgacgg cgctgcgtcg 60
tcgccgggat tcaagctcgt cggattttcc aagttcgtaa gaaagaatcc aaagtctgat 120
aaattcaagg ttaagcgctt ccatcacatc gagttctggt gcggcgacgc aaccaacgtc 180
gctcgtcgct tctcctgggg tctggggatg agattctccg ccaaatccga tctttccacc 240
ggaaacatgg ttcacgcctc ttacctactc acctccggtg acctccgatt ccttttcact 300
gctccttact ctccgtctct ctccgccgga gagattaaac cgacaaccac agcttctatc 360
ccaagtttcg atcacggctc ttgtcgttcc ttcttctctt cacatggtct cggtgttaga 420
gccgttgcga ttgaagtaga agacgcagag tcagctttct ccatcagtgt agctaatggc 480
gctattcctt cgtcgcctcc tatcgtcctc aatgaagcag ttacgatcgc tgaggttaaa 540
ctatacggcg atgttgttct ccgatatgtt agttacaaag cagaagatac cgaaaaatcc 600
gaattcttgc cagggttcga gcgtgtagag gatgcgtcgt cgttcccatt ggattatggt 660
atccggcggc ttgaccacgc cgtgggaaac gttcctgagc ttggtccggc tttaacttat 720
gtagcggggt tcactggttt tcaccaattc gcagagttca cagcagacga cgttggaacc 780
gccgagagcg gtttaaattc agcggtcctg gctagcaatg atgaaatggt tcttctaccg 840
attaacgagc cagtgcacgg aacaaagagg aagagtcaga ttcagacgta tttggaacat 900
aacgaaggcg cagggctaca acatctggct ctgatgagtg aagacatatt caggaccctg 960
agagagatga ggaagaggag cagtattgga ggattcgact tcatgccttc tcctccgcct 1020
acttactacc agaatctcaa gaaacgggtc ggcgacgtgc tcagcgatga tcagatcaag 1080
gagtgtgagg aattagggat tcttgtagac agagatgatc aagggacgtt gcttcaaatc 1140
ttcacaaaac cactaggtga caggccgacg atatatatag agataatcca gagagtagga 1200
tgcatgatga aagatgagga agggaaggct taccagagtg gaggatgtgg tggttttggc 1260
aaaggcaatt tctctgagct cttcaagtcc attgaagaat acgaaaagac tcttgaagcc 1320
aaacagttag tgggatga 1338
<210> 226
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F383Y-02核苷酸序列(Artificial Sequence)
<400> 226
atgggacatc agaatgctgc tgtatcagaa aatcaaaatc atgatgatgg agccgcctcc 60
tcaccagggt ttaagttggt tgggttctcc aagtttgtac ggaagaaccc aaaatccgac 120
aagttcaagg ttaaacgctt ccaccacatt gaattctggt gcggcgacgc caccaatgtt 180
gctcggcgtt tttcatgggg tttaggcatg agattctcag caaagagtga cctgagcacc 240
ggcaacatgg ttcatgctag ctatctctta acatctggag atttgaggtt tcttttcacg 300
gcgccgtact caccatcact ctccgccggc gagatcaaac ccaccacgac tgcaagcata 360
ccttcatttg atcatggtag ttgcagatcc ttcttctcgt cgcacggcct aggggtgcgc 420
gcggtcgcca tcgaagttga agatgcagaa tcagcgttta gtatcagcgt ggcaaatgga 480
gctattcctt cctcccctcc aattgtcctc aatgaggctg taactattgc cgaggtgaag 540
ttgtatggag atgttgtgct gagatatgtt tcttacaagg cagaggacac tgagaaatct 600
gaatttttgc ctggttttga aagggtcgag gatgcttcaa gttttcctct tgattacggc 660
atacgacggt tggatcacgc ggttggaaat gtaccagaac ttgggccggc gctcacttat 720
gtggctggtt tcactggatt ccatcaattt gctgagttca cagctgatga tgttgggact 780
gctgaaagtg ggttgaactc tgcagtcctc gcctctaatg atgaaatggt gctcctcccg 840
atcaacgagc ctgtgcatgg caccaaaagg aaatcacaaa tacagacata tcttgagcac 900
aatgaaggtg cagggcttca acatcttgca ctgatgtctg aagacatttt ccgtacgctg 960
agagagatga ggaagagaag tagtattggt ggatttgact tcatgccttc tcccccacca 1020
acttactacc agaacttgaa gaagagggtt ggtgatgtgc tatctgatga ccagatcaag 1080
gagtgtgaag aattgggcat tcttgttgat cgggacgacc aaggaacatt actgcagata 1140
tttacaaaac cacttggtga ccgccccact atttatattg agatcataca aagagtcggc 1200
tgcatgatga aggatgagga aggaaaagca tatcaatctg gtggttgtgg cggctttggg 1260
aaaggaaact tctctgagct gttcaaatcc atcgaggagt atgagaagac cttggaggcc 1320
aagcagctcg tgggttga 1338
<210> 227
<211> 445
<212> PRT
<213> 人工序列-AtHPPDm-F372A-F383Y氨基酸序列(Artificial Sequence)
<400> 227
Met Gly His Gln Asn Ala Ala Val Ser Glu Asn Gln Asn His Asp Asp
1 5 10 15
Gly Ala Ala Ser Ser Pro Gly Phe Lys Leu Val Gly Phe Ser Lys Phe
20 25 30
Val Arg Lys Asn Pro Lys Ser Asp Lys Phe Lys Val Lys Arg Phe His
35 40 45
His Ile Glu Phe Trp Cys Gly Asp Ala Thr Asn Val Ala Arg Arg Phe
50 55 60
Ser Trp Gly Leu Gly Met Arg Phe Ser Ala Lys Ser Asp Leu Ser Thr
65 70 75 80
Gly Asn Met Val His Ala Ser Tyr Leu Leu Thr Ser Gly Asp Leu Arg
85 90 95
Phe Leu Phe Thr Ala Pro Tyr Ser Pro Ser Leu Ser Ala Gly Glu Ile
100 105 110
Lys Pro Thr Thr Thr Ala Ser Ile Pro Ser Phe Asp His Gly Ser Cys
115 120 125
Arg Ser Phe Phe Ser Ser His Gly Leu Gly Val Arg Ala Val Ala Ile
130 135 140
Glu Val Glu Asp Ala Glu Ser Ala Phe Ser Ile Ser Val Ala Asn Gly
145 150 155 160
Ala Ile Pro Ser Ser Pro Pro Ile Val Leu Asn Glu Ala Val Thr Ile
165 170 175
Ala Glu Val Lys Leu Tyr Gly Asp Val Val Leu Arg Tyr Val Ser Tyr
180 185 190
Lys Ala Glu Asp Thr Glu Lys Ser Glu Phe Leu Pro Gly Phe Glu Arg
195 200 205
Val Glu Asp Ala Ser Ser Phe Pro Leu Asp Tyr Gly Ile Arg Arg Leu
210 215 220
Asp His Ala Val Gly Asn Val Pro Glu Leu Gly Pro Ala Leu Thr Tyr
225 230 235 240
Val Ala Gly Phe Thr Gly Phe His Gln Phe Ala Glu Phe Thr Ala Asp
245 250 255
Asp Val Gly Thr Ala Glu Ser Gly Leu Asn Ser Ala Val Leu Ala Ser
260 265 270
Asn Asp Glu Met Val Leu Leu Pro Ile Asn Glu Pro Val His Gly Thr
275 280 285
Lys Arg Lys Ser Gln Ile Gln Thr Tyr Leu Glu His Asn Glu Gly Ala
290 295 300
Gly Leu Gln His Leu Ala Leu Met Ser Glu Asp Ile Phe Arg Thr Leu
305 310 315 320
Arg Glu Met Arg Lys Arg Ser Ser Ile Gly Gly Phe Asp Phe Met Pro
325 330 335
Ser Pro Pro Pro Thr Tyr Tyr Gln Asn Leu Lys Lys Arg Val Gly Asp
340 345 350
Val Leu Ser Asp Asp Gln Ile Lys Glu Cys Glu Glu Leu Gly Ile Leu
355 360 365
Val Asp Arg Asp Asp Gln Gly Thr Leu Leu Gln Ile Ala Thr Lys Pro
370 375 380
Leu Gly Asp Arg Pro Thr Ile Tyr Ile Glu Ile Ile Gln Arg Val Gly
385 390 395 400
Cys Met Met Lys Asp Glu Glu Gly Lys Ala Tyr Gln Ser Gly Gly Cys
405 410 415
Gly Gly Phe Gly Lys Gly Asn Phe Ser Glu Leu Phe Lys Ser Ile Glu
420 425 430
Glu Tyr Glu Lys Thr Leu Glu Ala Lys Gln Leu Val Gly
435 440 445
<210> 228
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F372A-F383Y-01核苷酸序列(Artificial Sequence)
<400> 228
atgggccacc aaaacgccgc cgtttcagag aatcaaaacc atgatgacgg cgctgcgtcg 60
tcgccgggat tcaagctcgt cggattttcc aagttcgtaa gaaagaatcc aaagtctgat 120
aaattcaagg ttaagcgctt ccatcacatc gagttctggt gcggcgacgc aaccaacgtc 180
gctcgtcgct tctcctgggg tctggggatg agattctccg ccaaatccga tctttccacc 240
ggaaacatgg ttcacgcctc ttacctactc acctccggtg acctccgatt ccttttcact 300
gctccttact ctccgtctct ctccgccgga gagattaaac cgacaaccac agcttctatc 360
ccaagtttcg atcacggctc ttgtcgttcc ttcttctctt cacatggtct cggtgttaga 420
gccgttgcga ttgaagtaga agacgcagag tcagctttct ccatcagtgt agctaatggc 480
gctattcctt cgtcgcctcc tatcgtcctc aatgaagcag ttacgatcgc tgaggttaaa 540
ctatacggcg atgttgttct ccgatatgtt agttacaaag cagaagatac cgaaaaatcc 600
gaattcttgc cagggttcga gcgtgtagag gatgcgtcgt cgttcccatt ggattatggt 660
atccggcggc ttgaccacgc cgtgggaaac gttcctgagc ttggtccggc tttaacttat 720
gtagcggggt tcactggttt tcaccaattc gcagagttca cagcagacga cgttggaacc 780
gccgagagcg gtttaaattc agcggtcctg gctagcaatg atgaaatggt tcttctaccg 840
attaacgagc cagtgcacgg aacaaagagg aagagtcaga ttcagacgta tttggaacat 900
aacgaaggcg cagggctaca acatctggct ctgatgagtg aagacatatt caggaccctg 960
agagagatga ggaagaggag cagtattgga ggattcgact tcatgccttc tcctccgcct 1020
acttactacc agaatctcaa gaaacgggtc ggcgacgtgc tcagcgatga tcagatcaag 1080
gagtgtgagg aattagggat tcttgtagac agagatgatc aagggacgtt gcttcaaatc 1140
gctacaaaac cactaggtga caggccgacg atatatatag agataatcca gagagtagga 1200
tgcatgatga aagatgagga agggaaggct taccagagtg gaggatgtgg tggttttggc 1260
aaaggcaatt tctctgagct cttcaagtcc attgaagaat acgaaaagac tcttgaagcc 1320
aaacagttag tgggatga 1338
<210> 229
<211> 1338
<212> DNA
<213> 人工序列-AtHPPDm-F372A-F383Y-02核苷酸序列(Artificial Sequence)
<400> 229
atgggacatc agaatgctgc tgtatcagaa aatcaaaatc atgatgatgg agccgcctcc 60
tcaccagggt ttaagttggt tgggttctcc aagtttgtac ggaagaaccc aaaatccgac 120
aagttcaagg ttaaacgctt ccaccacatt gaattctggt gcggcgacgc caccaatgtt 180
gctcggcgtt tttcatgggg tttaggcatg agattctcag caaagagtga cctgagcacc 240
ggcaacatgg ttcatgctag ctatctctta acatctggag atttgaggtt tcttttcacg 300
gcgccgtact caccatcact ctccgccggc gagatcaaac ccaccacgac tgcaagcata 360
ccttcatttg atcatggtag ttgcagatcc ttcttctcgt cgcacggcct aggggtgcgc 420
gcggtcgcca tcgaagttga agatgcagaa tcagcgttta gtatcagcgt ggcaaatgga 480
gctattcctt cctcccctcc aattgtcctc aatgaggctg taactattgc cgaggtgaag 540
ttgtatggag atgttgtgct gagatatgtt tcttacaagg cagaggacac tgagaaatct 600
gaatttttgc ctggttttga aagggtcgag gatgcttcaa gttttcctct tgattacggc 660
atacgacggt tggatcacgc ggttggaaat gtaccagaac ttgggccggc gctcacttat 720
gtggctggtt tcactggatt ccatcaattt gctgagttca cagctgatga tgttgggact 780
gctgaaagtg ggttgaactc tgcagtcctc gcctctaatg atgaaatggt gctcctcccg 840
atcaacgagc ctgtgcatgg caccaaaagg aaatcacaaa tacagacata tcttgagcac 900
aatgaaggtg cagggcttca acatcttgca ctgatgtctg aagacatttt ccgtacgctg 960
agagagatga ggaagagaag tagtattggt ggatttgact tcatgccttc tcccccacca 1020
acttactacc agaacttgaa gaagagggtt ggtgatgtgc tatctgatga ccagatcaag 1080
gagtgtgaag aattgggcat tcttgttgat cgggacgacc aaggaacatt actgcagata 1140
gccacaaaac cacttggtga ccgccccact atttatattg agatcataca aagagtcggc 1200
tgcatgatga aggatgagga aggaaaagca tatcaatctg gtggttgtgg cggctttggg 1260
aaaggaaact tctctgagct gttcaaatcc atcgaggagt atgagaagac cttggaggcc 1320
aagcagctcg tgggttga 1338
<210> 230
<211> 24
<212> DNA
<213> 人工序列-5’端通用接头引物1(Artificial Sequence)
<400> 230
agtttttctg attaacagac tagt 24
<210> 231
<211> 25
<212> DNA
<213> 人工序列-3’端通用接头引物1(Artificial Sequence)
<400> 231
caaatgtttg aacgatcggc gcgcc 25
<210> 232
<211> 25
<212> DNA
<213> 人工序列-引物1(Artificial Sequence)
<400> 232
ctggaaggcg aggacgtcat caata 25
<210> 233
<211> 22
<212> DNA
<213> 人工序列-引物2(Artificial Sequence)
<400> 233
tggcggcatt gccgaaatcg ag 22
<210> 234
<211> 28
<212> DNA
<213> 人工序列-探针1(Artificial Sequence)
<400> 234
atgcaggcga tgggcgcccg catccgta 28
Claims (16)
1.一种突变的羟基苯丙酮酸双加氧酶多肽，其保留催化对羟基苯丙酮酸转化为尿黑酸或尿黑酸盐的活性，且对HPPD抑制剂除草剂的敏感性低于野生型HPPD，其特征在于，其在对应于SEQ ID NO：1所示的氨基酸序列包含如下位点处的氨基酸突变：在F372位被A、G或V取代且在F383位被W取代；
优选地，所述突变的羟基苯丙酮酸双加氧酶多肽在对应于SEQ ID NO：1所示的氨基酸序列包含如下位点处的氨基酸突变：在F372位被A取代且在F383位被W取代。
2.根据权利要求1所述突变的羟基苯丙酮酸双加氧酶多肽，其特征在于，所述突变的羟基苯丙酮酸双加氧酶多肽还包含第二突变；
优选地，所述第二突变在对应于SEQ ID NO：1所示的氨基酸序列包含如下至少一个位点处的氨基酸突变：A106G、A107缺失、A111T或K351N。
3.根据权利要求1或2所述突变的羟基苯丙酮酸双加氧酶多肽，其特征在于，所述突变的羟基苯丙酮酸双加氧酶多肽包括：
具有SEQ ID NO：173、SEQ ID NO：182、SEQ ID NO：185、SEQ ID NO：188、SEQ ID NO：191、SEQ ID NO：194、SEQ ID NO：197、SEQ ID NO：200或SEQ ID NO：203所示的氨基酸序列组成的多肽。
4.根据权利要求1-3任一项所述突变的羟基苯丙酮酸双加氧酶多肽，其特征在于，所述突变的羟基苯丙酮酸双加氧酶多肽衍生自植物野生型HPPD或微生物野生型HPPD。
5.一种编码权利要求1-4任一项所述突变的羟基苯丙酮酸双加氧酶多肽的多核苷酸。
6.一种表达盒或重组载体，其特征在于，包含在有效连接的调控序列调控下的权利要求5所述多核苷酸。
7.一种扩展植物耐受的除草剂范围的方法，其特征在于，包括：将权利要求1-4任一项所述突变的羟基苯丙酮酸双加氧酶多肽与至少一种不同于权利要求1-4任一项所述突变的羟基苯丙酮酸双加氧酶多肽的除草剂耐受性蛋白质一起表达。
8.根据权利要求7所述扩展植物耐受的除草剂范围的方法，其特征在于，所述除草剂耐受性蛋白质为5-烯醇丙酮酰莽草酸-3-磷酸合酶、草甘膦氧化还原酶、草甘膦-N-乙酰转移酶、草甘膦脱羧酶、草铵膦乙酰转移酶、α酮戊二酸依赖性双加氧酶、麦草畏单加氧酶、乙酰乳酸合酶、细胞色素类蛋白质和/或原卟啉原氧化酶。
9.一种选择转化的植物细胞的方法，其特征在于，包括：用权利要求5所述多核苷酸转化多个植物细胞，并在允许表达所述多核苷酸的转化细胞生长，而杀死未转化细胞或抑制未转化细胞生长的HPPD抑制剂除草剂浓度下培养所述细胞；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮。
10.一种控制杂草的方法，其特征在于，包括：对种植目的植物的大田施用有效剂量的HPPD抑制剂除草剂，所述目的植物包含权利要求5所述多核苷酸；
优选地，所述目的植物为草甘膦耐受性植物，所述杂草为草甘膦抗性杂草；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮，。
11.一种用于保护植物免受由HPPD抑制剂除草剂引起的损伤或赋予植物HPPD抑制剂除草剂耐受性的方法，其特征在于，包括：将权利要求5所述多核苷酸或权利要求6所述表达盒或所述重组载体导入植物，使导入后的植物产生足够保护其免受HPPD抑制剂除草剂损害量的所述突变的羟基苯丙酮酸双加氧酶多肽；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮。
12.一种产生耐受HPPD抑制剂除草剂植物的方法，其特征在于，包括向植物的基因组中引入权利要求5所述多核苷酸；
优选地，所述引入的方法包括遗传转化方法、基因组编辑方法或基因突变方法；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮。
13.一种培养耐受HPPD抑制剂除草剂植物的方法，其特征在于，包括：
种植至少一个植物繁殖体，所述植物繁殖体的基因组中包括权利要求5所述多核苷酸；
使所述植物繁殖体长成植株；
将有效剂量的HPPD抑制剂除草剂施加到至少包含所述植株的植物生长环境中，收获与其他不具有权利要求5所述多核苷酸的植株相比具有减弱的植物损伤和/或具有增加的植物产量的植株；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮。
14.一种获得加工农产品的方法，其特征在于，包括将由权利要求13所述方法获得的耐受HPPD抑制剂除草剂植物的收获物进行处理以获得加工农产品。
15.一种控制杂草生长的种植系统，其特征在于，包括HPPD抑制剂除草剂和存在至少一种目的植物的植物生长环境，所述目的植物包含权利要求5所述多核苷酸；
优选地，所述目的植物为草甘膦耐受性植物，所述杂草为草甘膦抗性杂草；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮。
16.一种权利要求1-4任一项所述突变的羟基苯丙酮酸双加氧酶多肽在赋予植物HPPD抑制剂除草剂耐受性中的用途；
优选地，所述HPPD抑制剂除草剂包括吡唑啉酮类HPPD抑制剂除草剂、三酮类HPPD抑制剂除草剂和/或异噁唑类HPPD抑制剂除草剂；更优选地，所述吡唑啉酮类HPPD抑制剂除草剂为苯吡唑草酮，所述异噁唑类HPPD抑制剂除草剂为异噁唑草酮，所述三酮类HPPD抑制剂除草剂为甲基磺草酮。
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| PCT/CN2021/138425 WO2023108495A1 (zh) | 2021-12-15 | 2021-12-15 | 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途 |
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| AU2021479356A1 (en) | 2024-05-23 |
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| AR127984A1 (es) | 2024-03-13 |
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