BR112016017606A2 - superexpressão de reguladores de via de n-glicosilação para modular a glicosilação de proteínas recombinantes - Google Patents

superexpressão de reguladores de via de n-glicosilação para modular a glicosilação de proteínas recombinantes

Info

Publication number
BR112016017606A2
BR112016017606A2 BR112016017606A BR112016017606A BR112016017606A2 BR 112016017606 A2 BR112016017606 A2 BR 112016017606A2 BR 112016017606 A BR112016017606 A BR 112016017606A BR 112016017606 A BR112016017606 A BR 112016017606A BR 112016017606 A2 BR112016017606 A2 BR 112016017606A2
Authority
BR
Brazil
Prior art keywords
glycosylation
overexpression
modulate
recombinant protein
pathway regulators
Prior art date
Application number
BR112016017606A
Other languages
English (en)
Portuguese (pt)
Inventor
Gupta Shivani
Kang Sohye
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53757626&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112016017606(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of BR112016017606A2 publication Critical patent/BR112016017606A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01101—Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01143—Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01094—Protein N-acetylglucosaminyltransferase (2.4.1.94)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
BR112016017606A 2014-01-29 2014-12-11 superexpressão de reguladores de via de n-glicosilação para modular a glicosilação de proteínas recombinantes BR112016017606A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461933137P 2014-01-29 2014-01-29
PCT/US2014/069744 WO2015116315A1 (en) 2014-01-29 2014-12-11 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins

Publications (1)

Publication Number Publication Date
BR112016017606A2 true BR112016017606A2 (pt) 2017-10-10

Family

ID=53757626

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016017606A BR112016017606A2 (pt) 2014-01-29 2014-12-11 superexpressão de reguladores de via de n-glicosilação para modular a glicosilação de proteínas recombinantes

Country Status (14)

Country Link
US (3) US10227627B2 (https=)
EP (1) EP3099806A1 (https=)
JP (4) JP6474420B2 (https=)
KR (3) KR102519540B1 (https=)
CN (2) CN106170554B (https=)
AU (3) AU2014380174C1 (https=)
BR (1) BR112016017606A2 (https=)
CA (1) CA2938079C (https=)
CL (1) CL2016001900A1 (https=)
EA (2) EA037565B1 (https=)
IL (3) IL282517B (https=)
MX (2) MX377780B (https=)
SG (1) SG10201912081RA (https=)
WO (1) WO2015116315A1 (https=)

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KR101936049B1 (ko) 2015-10-15 2019-01-08 (주)알테오젠 IgG Fc 도메인을 가지는 융합 단백질의 생산방법
CA3056011A1 (en) * 2017-03-14 2018-09-20 Amgen Inc. Control of total afucosylated glycoforms of antibodies produced in cell culture
IL307155B2 (en) 2018-05-01 2025-11-01 Amgen Inc Antibodies with modulated glycan profiles
CN109576212B (zh) * 2018-12-14 2021-06-22 杭州奕安济世生物药业有限公司 高密度接种培养中种子细胞的培养方法及其应用
EP3980524A4 (en) * 2019-06-10 2023-01-11 Cho Plus Inc. Cell lines for high level production of protein-based pharmaceuticals
EP4326766A1 (en) 2021-04-23 2024-02-28 Amgen Inc. Anti-tslp antibody compositions and uses thereof
EP4334356A1 (en) * 2021-05-03 2024-03-13 Boehringer Ingelheim International Gmbh Method for producing spesolimab
CN113684322B (zh) * 2021-09-09 2024-01-23 上海药明生物技术有限公司 一种降低抗体类蛋白高聚甘露糖型水平的方法
CN114525252B (zh) * 2022-03-10 2023-12-01 广州源井生物科技有限公司 一种提高mda-mb-231单细胞克隆形成率的单克隆增强培养基与培养方法及其应用
TW202426485A (zh) 2022-10-26 2024-07-01 美商安進公司 抗ｔｓｌｐ抗體組成物及其用途

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Also Published As

Publication number Publication date
CN106170554A (zh) 2016-11-30
JP7034236B2 (ja) 2022-03-11
CA2938079C (en) 2022-10-11
CN106170554B (zh) 2021-08-17
AU2014380174A1 (en) 2016-08-11
CN113774084A (zh) 2021-12-10
KR102410393B1 (ko) 2022-06-16
AU2018264100A1 (en) 2018-12-06
JP6474420B2 (ja) 2019-02-27
AU2018264100B2 (en) 2020-07-23
US20200277642A1 (en) 2020-09-03
AU2014380174C1 (en) 2025-06-05
IL246914B (en) 2019-12-31
AU2020239681C1 (en) 2025-06-05
IL270861B (en) 2021-05-31
CL2016001900A1 (es) 2017-05-12
IL282517A (en) 2021-06-30
US10655156B2 (en) 2020-05-19
JP6797950B2 (ja) 2020-12-09
KR102301034B1 (ko) 2021-09-13
EA037565B1 (ru) 2021-04-14
US10227627B2 (en) 2019-03-12
KR20210114549A (ko) 2021-09-23
JP7788307B2 (ja) 2025-12-18
AU2020239681A1 (en) 2020-10-15
AU2018264100C1 (en) 2025-06-05
IL282517B (en) 2022-07-01
US10907186B2 (en) 2021-02-02
KR102519540B1 (ko) 2023-04-10
JP2021040638A (ja) 2021-03-18
MX2016009911A (es) 2016-10-28
SG10201912081RA (en) 2020-02-27
EA201691532A1 (ru) 2017-01-30
JP2022068365A (ja) 2022-05-09
EP3099806A1 (en) 2016-12-07
US20190153496A1 (en) 2019-05-23
JP2017505620A (ja) 2017-02-23
AU2020239681B2 (en) 2022-06-23
IL246914A0 (en) 2016-09-29
CA2938079A1 (en) 2015-08-06
WO2015116315A1 (en) 2015-08-06
KR20160111943A (ko) 2016-09-27
EA202190244A2 (ru) 2021-05-31
MX2020012356A (es) 2021-01-29
KR20220086703A (ko) 2022-06-23
CN113774084B (zh) 2024-04-16
JP2019088305A (ja) 2019-06-13
EA202190244A3 (ru) 2021-08-31
US20190010532A1 (en) 2019-01-10
MX377780B (es) 2025-03-11
IL270861A (en) 2020-01-30
AU2014380174B2 (en) 2018-08-30

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Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]