BR112015026306A2 - método para separação e purificação da lactoferrina humana recombinante das sementes de arroz - Google Patents

método para separação e purificação da lactoferrina humana recombinante das sementes de arroz

Info

Publication number
BR112015026306A2
BR112015026306A2 BR112015026306A BR112015026306A BR112015026306A2 BR 112015026306 A2 BR112015026306 A2 BR 112015026306A2 BR 112015026306 A BR112015026306 A BR 112015026306A BR 112015026306 A BR112015026306 A BR 112015026306A BR 112015026306 A2 BR112015026306 A2 BR 112015026306A2
Authority
BR
Brazil
Prior art keywords
purification
separation
recombinant human
rice seeds
human lactoferrin
Prior art date
Application number
BR112015026306A
Other languages
English (en)
Other versions
BR112015026306B1 (pt
Inventor
Yang Daichang
SHI Jingni
Ou Jiquan
Original Assignee
Wuhan Healthgen Biotechnology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wuhan Healthgen Biotechnology Corp filed Critical Wuhan Healthgen Biotechnology Corp
Publication of BR112015026306A2 publication Critical patent/BR112015026306A2/pt
Publication of BR112015026306B1 publication Critical patent/BR112015026306B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

resumo o presente invento fornece um método cromatográfico de separação e purificação da lactoferrina humana recombinante (rlf) a partir das sementes de arroz transgênico, constituído das seguintes etapas: (1) uso dos sementes de arroz transgênico com lactoferrina humana recombinante como matéria-prima, com a utilização de um tampão de extração para preparação de um extrato bruto de rlf; e (2) separação e purificação do extrato bruto de rlf por cromatografia de troca catiônica, para se obter uma amostra de rlf purificada como objeto de estudo. o método cromatográfico consiste também em uma etapa para a resina regenerada.
BR112015026306-2A 2013-04-16 2014-04-01 método para separação e purificação da lactoferrina humana recombinante das sementes de arroz BR112015026306B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201310131488.7 2013-04-16
CN201310131488.7A CN104109204B (zh) 2013-04-16 2013-04-16 一种从水稻种子中分离纯化重组人乳铁蛋白的方法
PCT/CN2014/074539 WO2014169760A1 (zh) 2013-04-16 2014-04-01 一种从水稻种子中分离纯化重组人乳铁蛋白的方法

Publications (2)

Publication Number Publication Date
BR112015026306A2 true BR112015026306A2 (pt) 2017-07-25
BR112015026306B1 BR112015026306B1 (pt) 2020-12-29

Family

ID=51706206

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015026306-2A BR112015026306B1 (pt) 2013-04-16 2014-04-01 método para separação e purificação da lactoferrina humana recombinante das sementes de arroz

Country Status (12)

Country Link
EP (1) EP2987800B1 (pt)
JP (1) JP6353522B2 (pt)
KR (1) KR102106583B1 (pt)
CN (1) CN104109204B (pt)
BR (1) BR112015026306B1 (pt)
CA (1) CA2907892C (pt)
CL (1) CL2015003069A1 (pt)
DK (1) DK2987800T3 (pt)
ES (1) ES2668719T3 (pt)
MX (1) MX366198B (pt)
WO (1) WO2014169760A1 (pt)
ZA (1) ZA201507716B (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105238858B (zh) * 2015-09-25 2019-02-05 浙江大学 一种用于检测转重组人乳铁蛋白水稻品系g281的侧翼序列及其特异性鉴定方法
CN111138524B (zh) * 2018-11-02 2023-07-07 武汉禾元生物科技股份有限公司 一种从基因工程水稻种子中分离纯化低铁饱和度的重组人乳铁蛋白的方法
CN111285932B (zh) * 2018-12-10 2023-07-11 武汉禾元生物科技股份有限公司 一种从基因工程水稻种子中分离纯化重组人纤维连接蛋白的方法
CN111285929B (zh) * 2018-12-10 2023-09-19 武汉禾元生物科技股份有限公司 一种从基因工程水稻种子中分离纯化重组人表皮细胞生长因子的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2584727B1 (fr) * 1985-07-11 1988-06-17 Roussel Uclaf Procede d'extraction de proteines du lait, produits, application du procede, et compositions pharmaceutiques
IE61701B1 (en) * 1986-07-17 1994-11-30 Morinaga Milk Industry Co Ltd Process for producing bovine lactoferrin in high purity
JPH10323137A (ja) * 1997-03-24 1998-12-08 Allergen Free Technol Kenkyusho:Kk ラクトフェリン機能を食用植物に付与する方法、及びラクトフェリン機能を有する食用植物
US7417178B2 (en) * 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
EP1605772B1 (en) * 2003-03-21 2007-06-27 Upfront Chromatography A/S Method for high throughput volumes in the fractionation of bio-molecules by chromatographic systems
CN1663961A (zh) * 2004-09-29 2005-09-07 天津商学院 牛初乳中乳铁蛋白的分离纯化技术
CN102847141A (zh) * 2005-09-28 2013-01-02 文特里亚生物科学公司 用于肠道病症和/或补水的口服制剂
CN100379755C (zh) * 2006-03-29 2008-04-09 浙江大学 从转基因人乳铁蛋白水稻中提取人乳铁蛋白的方法
JP4246777B1 (ja) * 2007-11-20 2009-04-02 森永乳業株式会社 異常型プリオン蛋白質結合剤および異常型プリオン蛋白質の検出方法
CN102382168A (zh) * 2010-09-03 2012-03-21 中国科学院过程工程研究所 一种高效纯化金属离子结合蛋白的离子交换层析分离纯化方法

Also Published As

Publication number Publication date
ES2668719T3 (es) 2018-05-21
WO2014169760A1 (zh) 2014-10-23
DK2987800T3 (en) 2018-06-14
MX2015014193A (es) 2016-02-10
ZA201507716B (en) 2017-11-29
CL2015003069A1 (es) 2017-01-20
JP6353522B2 (ja) 2018-07-04
EP2987800B1 (en) 2018-03-07
KR20150143765A (ko) 2015-12-23
KR102106583B1 (ko) 2020-05-04
EP2987800A1 (en) 2016-02-24
EP2987800A4 (en) 2016-12-14
BR112015026306B1 (pt) 2020-12-29
CN104109204A (zh) 2014-10-22
CA2907892C (en) 2021-01-19
MX366198B (es) 2019-06-26
JP2016517850A (ja) 2016-06-20
CN104109204B (zh) 2017-11-07
CA2907892A1 (en) 2014-10-23

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NAO 10196/2001, QUE MODIFICOU A LEI NAO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUAANCIA PRA VIA DA ANVISA. CONSIDERANDO A APROVAA AO DOS TERMOS DO PARECER NAO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NAO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDAANCIAS CABA-VEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 01/04/2014, OBSERVADAS AS CONDICOES LEGAIS.