BR112015022495A8 - Cassete de expressão e método para expressar uma sequência nucleotídica em uma célula vegetal - Google Patents

Cassete de expressão e método para expressar uma sequência nucleotídica em uma célula vegetal

Info

Publication number
BR112015022495A8
BR112015022495A8 BR112015022495A BR112015022495A BR112015022495A8 BR 112015022495 A8 BR112015022495 A8 BR 112015022495A8 BR 112015022495 A BR112015022495 A BR 112015022495A BR 112015022495 A BR112015022495 A BR 112015022495A BR 112015022495 A8 BR112015022495 A8 BR 112015022495A8
Authority
BR
Brazil
Prior art keywords
nucleotide sequence
plant
promoter
plant cell
nucleotide sequences
Prior art date
Application number
BR112015022495A
Other languages
English (en)
Other versions
BR112015022495B1 (pt
BR112015022495A2 (pt
Inventor
Zhang Shirong
Original Assignee
Bayer Cropscience Lp
Basf Se
BASF Agricultural Solutions Seed US LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Cropscience Lp, Basf Se, BASF Agricultural Solutions Seed US LLC filed Critical Bayer Cropscience Lp
Publication of BR112015022495A2 publication Critical patent/BR112015022495A2/pt
Publication of BR112015022495A8 publication Critical patent/BR112015022495A8/pt
Publication of BR112015022495B1 publication Critical patent/BR112015022495B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)

Abstract

PROMOTORES DE FEIJÃO-SOJA CONSTITUTIVOS. A presente invenção fornece composições e método para regular a expressão de sequências nucleotídicas heterólogos em uma planta. As composições incluem duas sequências nucleotídicas promotores inovadoras para os genes que codificam proteína gama intrínseca de tonoplasto e proteína intrínseca de membrana plasmática em feijão-soja, assim como vetores, micro-organismos, plantas e células vegetais que compreende as sequências nucleotídicas promotores, ou variantes e fragmentos das mesmas. Os métodos para expressar uma sequência nucleotídica heteróloga em uma planta com o uso das sequências de promotores reveladas no presente documento também são fornecidos. Os métodos compreendem incorporar estavelmente no genoma de uma célula vegetal uma sequência nucleotídica ligada de maneira funcional ao promotor da presente invenção e regenerar uma planta transformada estavelmente que expressa a sequência nucleotídica.
BR112015022495-4A 2013-03-15 2014-03-11 Promotores de feijão-soja constitutivos BR112015022495B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361790907P 2013-03-15 2013-03-15
US61/790,907 2013-03-15
US2014150449 2014-09-25

Publications (3)

Publication Number Publication Date
BR112015022495A2 BR112015022495A2 (pt) 2017-10-24
BR112015022495A8 true BR112015022495A8 (pt) 2022-06-28
BR112015022495B1 BR112015022495B1 (pt) 2024-08-20

Family

ID=

Also Published As

Publication number Publication date
WO2014150449A2 (en) 2014-09-25
JP6463724B2 (ja) 2019-02-06
CA2903226C (en) 2022-03-15
AU2014237167A1 (en) 2015-10-01
RU2015144014A (ru) 2017-04-21
JP2016514963A (ja) 2016-05-26
CN105102474B (zh) 2019-11-12
EP2970405A2 (en) 2016-01-20
CN105102474A (zh) 2015-11-25
CA2903226A1 (en) 2014-09-25
RU2714724C2 (ru) 2020-02-19
US20150376643A1 (en) 2015-12-31
AU2014237167B2 (en) 2018-07-12
UA118845C2 (uk) 2019-03-25
US9944938B2 (en) 2018-04-17
AR095472A1 (es) 2015-10-21
ES2702289T3 (es) 2019-02-28
BR112015022495A2 (pt) 2017-10-24
WO2014150449A3 (en) 2014-11-13
MX359956B (es) 2018-10-16
EP2970405B1 (en) 2018-09-12
MX2015012171A (es) 2015-11-30

Similar Documents

Publication Publication Date Title
MX2015012171A (es) Promotores constitutivos de soja.
BR112018012887A2 (pt) molécula de ácido nucleico, cassete de expressão, vetor, célula vegetal, planta, método para expressar um polinucleotídeo
CO7151532A2 (es) Métodos para mejorar la seguridad del transporte de la barrera hematoencefálica
HRP20211170T1 (hr) Pripravci i postupci koji se odnose na mutantni toksin iz bakterije clostridium difficile
BR112014007115A2 (pt) éster sintases de cera acil-acp
BR112017017260A2 (pt) construções de dna, vetor, célula, plantas, semente, método de expressão de rna e método de modificação de local alvo
BR112015013029A2 (pt) promotores e terminadores de tetraselmis para uso em células eucarióticas
EA201390592A1 (ru) Аллели 1-d-дезоксиксилулозо-5-фосфат синтазы, ответственные за усиленный биосинтез терпенов
BR112018005629A2 (pt) promotor de planta e 3' utr para expressão de transgene
BR112015002918B8 (pt) Construção de ácido nucleico compreendeendo uma região não traduzida, vetor e métodos para produção de um peptídeo ou uma proteína e para a expressão de um gene em uma planta ou células vegetais
IN2014DN10111A (pt)
BR112015021546A2 (pt) molécula de ácido nucleico isolada, cassete de expressão, vetor, célula vegetal, planta, semente transgênica, método para expressar uma sequência de genes em uma planta ou uma célula vegetal e método para expressão de uma sequência de nucleotídeos de uma maneira preferencial para raiz em uma planta
BR112014019166A2 (pt) promotores para expressão aumentada de proteína em meningococos
WO2012070795A3 (ko) 고구마 유래의 ＩｂＯｒ-Ｉｎｓ 유전자 변이체 및 이의 용도
BR112013031709A2 (pt) sequência de ácidos nucleicos isolada, sintética ou recombinante, gene quimérico, vetor, célula hospedeira, polipeptídeo, métodos para aumentar produção de pelo menos um terpeno, para reduzir produção de pelo menos um terpeno, para reduzir níveis de terpeno, para produzir um terpeno, isômero de terpeno, ou análogo de terpeno, e para uma introgressão auxiliada pelo marcador de produção de pelo menos um terpeno, planta, e, cultura de tecido
MX2016008850A (es) Procedimiento para aumentar la biomasa y el contenido de lipidos de un microorganismo y microorganismo geneticamente modificado que muestra una autofagia potenciada.
BR112018003012A2 (pt) promotor de planta e 3? utr para expressão de transgene
BR112015021562A2 (pt) molécula de ácido nucleico isolada, cassete de expressão, vetor, célula vegetal, planta transgênica, semente transgênica, método para expressar uma sequência de nucleotídeos em uma planta ou uma célula vegetal, método para expressão de uma sequência gênica de maneira preferencial para raiz em uma planta
BRPI1008762B8 (pt) métodos para seleção de células de mamífero expressando uma proteína heteróloga, para produzir um produto de interesse e uso de um meio de cultura seletivo
BR112018008118B1 (pt) Promotor que se induz sob condições de estresse hídrico, construção de dna, vetor de expressão, célula de micro organismo transformada e método para expressar uma sequência de nucleotídeos
UY35783A (es) Elementos regulatorios de zea mays y usos de los mismos.
AR108749A1 (es) Promotor vegetal y 3’ utr para la expresión de transgenes
BR112019005600A2 (pt) promotor de planta para expressão de transgene
BR112018005556A2 (pt) promotor de planta e 3' utr para expressão de transgene
BR112014029968A2 (pt) promotores induzíveis de plantas e seu uso

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B25A Requested transfer of rights approved

Owner name: BASF SE (DE)

B25A Requested transfer of rights approved

Owner name: BASF AGRICULTURAL SOLUTIONS SEED US LLC (US)

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 11/03/2014, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO.