BR112015013029A2 - promotores e terminadores de tetraselmis para uso em células eucarióticas - Google Patents

promotores e terminadores de tetraselmis para uso em células eucarióticas

Info

Publication number
BR112015013029A2
BR112015013029A2 BR112015013029A BR112015013029A BR112015013029A2 BR 112015013029 A2 BR112015013029 A2 BR 112015013029A2 BR 112015013029 A BR112015013029 A BR 112015013029A BR 112015013029 A BR112015013029 A BR 112015013029A BR 112015013029 A2 BR112015013029 A2 BR 112015013029A2
Authority
BR
Brazil
Prior art keywords
tetraselmis
eukaryotic cells
terminators
promoters
gene
Prior art date
Application number
BR112015013029A
Other languages
English (en)
Inventor
Edwards Amanda
Liu Bo
R Moellering Eric
C Schneider Jane
H Verruto John
Lieberman Soyan
Original Assignee
Exxonmobil Res & Eng Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exxonmobil Res & Eng Co filed Critical Exxonmobil Res & Eng Co
Publication of BR112015013029A2 publication Critical patent/BR112015013029A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

resumo patente de invenção: "promotores e terminadores de tetraselmis para uso em células eucarióticas". a presente invenção refere-se a novas sequências promotoras e terminadoras para uso na expressão gênica nas células eucarióticas, tais como células de algas. a invenção fornece ainda cassetes de expressão que compreendem um promotor, como descrito aqui, ligado de forma operacional a um gene. a invenção fornece ainda vetores de expressão e células eucarióticas hospedeiras, tais como células de algas, para a expressão de uma proteína codificada pelo gene; e métodos para transformar de forma estável algas eucarióticas tal como tetraselmis com transgenes.
BR112015013029A 2012-12-04 2012-12-04 promotores e terminadores de tetraselmis para uso em células eucarióticas BR112015013029A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2012/067788 WO2014088560A1 (en) 2012-12-04 2012-12-04 Tetraselmis promoters and terminators for use in eukaryotic cells
US13/693,585 US8883993B2 (en) 2012-12-04 2012-12-04 Tetraselmis promoters and terminators for use in eukaryotic cells

Publications (1)

Publication Number Publication Date
BR112015013029A2 true BR112015013029A2 (pt) 2017-09-12

Family

ID=50825820

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015013029A BR112015013029A2 (pt) 2012-12-04 2012-12-04 promotores e terminadores de tetraselmis para uso em células eucarióticas

Country Status (6)

Country Link
US (1) US8883993B2 (pt)
EP (1) EP2929030B1 (pt)
AU (1) AU2012396282B2 (pt)
BR (1) BR112015013029A2 (pt)
CA (1) CA2893692C (pt)
WO (1) WO2014088560A1 (pt)

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CN104754954B (zh) 2012-08-23 2018-08-28 特郎萨格以色列有限公司 转基因微藻和其用于口服递送蛋白质的用途
CN106029091B (zh) 2014-02-12 2020-02-11 特郎萨格以色列有限公司 基于藻类的可食性疫苗
BR112017001229A2 (pt) 2014-07-25 2017-11-28 Benson Hill Biosystems Inc composições e métodos para aumentar o rendimento e o crescimento vegetal com o uso de promotores de arroz
WO2016039300A1 (ja) * 2014-09-08 2016-03-17 公立大学法人兵庫県立大学 珪藻の新規形質転換ベクターおよびその含有する新規プロモーター配列
AU2015373893B2 (en) 2014-12-31 2021-07-29 Synthetic Genomics, Inc. Compositions and methods for high efficiency in vivo genome editing
EP3265567B1 (en) 2015-03-02 2020-07-29 Conagen Inc. Regulatory elements from labyrinthulomycetes microorganisms
US10544424B2 (en) 2015-04-15 2020-01-28 Synthetic Genomics, Inc. Algal chloroplastic SRP54 mutants
EP3430035A4 (en) 2016-03-16 2020-02-19 Conagen Inc. PRODUCTION OF PROTEINS IN LABYRINE THULOMYCETES
EP3246401A1 (en) 2016-05-20 2017-11-22 Commissariat À L'Énergie Atomique Et Aux Énergies Alternatives New fatty acid decarboxylase and its uses
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
US11124798B2 (en) 2017-12-08 2021-09-21 Synthetic Genomics, Inc. Algal lipid productivity via genetic modification of a TPR domain containing protein
US11193132B2 (en) 2017-12-29 2021-12-07 Synthetic Genomics, Inc. Genetic modulation of photosynthetic organisms for improved growth
CN111467484A (zh) * 2020-03-09 2020-07-31 华东理工大学 一种广谱型微藻口服疫苗及其制备方法和应用
CN112592954B (zh) * 2020-12-22 2022-09-06 广东省微生物研究所（广东省微生物分析检测中心） 基因GliT作为筛选标记基因在抗性筛选中的应用

Family Cites Families (10)

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DK0538496T3 (da) * 1991-08-26 2004-02-23 Baxter Healthcare Sa Rekombinant fjerkrækoppevirus med intakt FPV tk-gen
WO2000062601A1 (en) 1999-04-15 2000-10-26 Northeastern University Agrobacterium-mediated genetic transformation of multicellular marine algae, resultant strains and their products
US20070016976A1 (en) * 2000-06-23 2007-01-18 Fumiaki Katagiri Plant genes involved in defense against pathogens
US7678561B2 (en) 2006-05-09 2010-03-16 The Scripps Research Institute Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast
US8076305B2 (en) * 2006-11-30 2011-12-13 Medtronic, Inc. Biological pacemakers including mutated hyperpolarization-activated cyclic nucleotide-gated (HCN) channels
WO2009036385A2 (en) * 2007-09-12 2009-03-19 Kuehnle Agrosystems, Inc. Expression of nucleic acid sequences for production of biofuels and other products in algae and cyanobacteria
EP2231864B1 (en) * 2007-12-11 2017-05-24 Synthetic Genomics, Inc. Secretion of fatty acids by photosynthetic microorganisms
AU2009231810A1 (en) * 2008-03-31 2009-10-08 Kuehnle Agrosystems, Inc. Nuclear based expression of genes for production of biofuels and process co-products in algae
WO2011034863A1 (en) 2009-09-15 2011-03-24 Sapphire Energy, Inc. A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
WO2012087676A2 (en) * 2010-12-23 2012-06-28 Exxonmobil Research And Enginnering Company Production of fatty acids and fatty acid derivatives by recombinant microorganisms expressing polypeptides having lipolytic activity

Also Published As

Publication number Publication date
CA2893692C (en) 2019-06-25
AU2012396282B2 (en) 2016-05-19
EP2929030A1 (en) 2015-10-14
AU2012396282A1 (en) 2015-06-11
WO2014088560A1 (en) 2014-06-12
US20140154806A1 (en) 2014-06-05
US8883993B2 (en) 2014-11-11
EP2929030B1 (en) 2018-10-31
CA2893692A1 (en) 2014-06-12
EP2929030A4 (en) 2017-02-01

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements