BR112014016777A2 - molécula de ácido nucléico isolada, cassete de expressão, vetor, célula vegetal, planta, semente transgênica e métodos para expressão de um polinucleotídeo - Google Patents

molécula de ácido nucléico isolada, cassete de expressão, vetor, célula vegetal, planta, semente transgênica e métodos para expressão de um polinucleotídeo

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Publication number
BR112014016777A2
BR112014016777A2 BR112014016777A BR112014016777A BR112014016777A2 BR 112014016777 A2 BR112014016777 A2 BR 112014016777A2 BR 112014016777 A BR112014016777 A BR 112014016777A BR 112014016777 A BR112014016777 A BR 112014016777A BR 112014016777 A2 BR112014016777 A2 BR 112014016777A2
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BR
Brazil
Prior art keywords
expression
plant
polynucleotide
vector
nucleic acid
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BR112014016777A
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English (en)
Inventor
A Chamberlin Mark
J Lawit Shai
Original Assignee
Pioneer Hi Bred Int
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Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of BR112014016777A2 publication Critical patent/BR112014016777A2/pt

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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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BR112014016777A 2012-01-06 2012-04-12 molécula de ácido nucléico isolada, cassete de expressão, vetor, célula vegetal, planta, semente transgênica e métodos para expressão de um polinucleotídeo BR112014016777A2 (pt)

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GB2537153A (en) * 2015-04-09 2016-10-12 Stichting Diesnt Landbouwkundig Onderzoek Induction of somatic embryogenesis in plants
WO2017155714A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
CN109477117A (zh) * 2016-04-11 2019-03-15 巴斯夫农业种子解决方案美国有限责任公司 种子特异性和胚乳优选性启动子及其用途
AU2017286122A1 (en) 2016-06-14 2018-11-22 Pioneer Hi-Bred International, Inc. Use of Cpf1 endonuclease for plant genome modifications
WO2017222773A1 (en) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
BR112019005687A2 (pt) 2016-10-03 2019-07-02 Dow Agrosciences Llc promotor vegetal para expressão de transgenes
CA3038508A1 (en) 2016-10-03 2018-04-12 Dow Agrosciences Llc Plant promoter for transgene expression
WO2018098420A1 (en) * 2016-11-28 2018-05-31 The Regents Of The University Of California Induction of haploid plants using baby boom1 gene
US11447787B2 (en) 2017-04-10 2022-09-20 The Regents Of The University Of California Generation of haploid plants
BR112019022201A2 (pt) 2017-04-24 2020-05-12 Dupont Nutrition Biosciences Aps Métodos para modular a atividade de uma endonuclease cas, para aumentar a especificidade de uma endonuclease cas e complexo polinucleotídico guia, para aumentar a frequência de recombinação homóloga e para ativação ou repressão gênica, célula e célula vegetal
BR112019027401A2 (pt) 2017-06-28 2020-07-14 Dow Agrosciences Llc promotor de planta para expressão de transgene
US11236350B2 (en) 2017-10-04 2022-02-01 Corteva Agriscience Llc Plant promoter for transgene expression
CN112143716B (zh) * 2020-09-30 2022-07-15 中国农业科学院棉花研究所 硫醇还原酶类基因或其编码的蛋白在调控植物表皮毛性能方面的应用

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US20080184386A1 (en) * 1998-11-16 2008-07-31 Yongwei Cao Plant genome sequences and uses thereof
US9029523B2 (en) * 2000-04-26 2015-05-12 Ceres, Inc. Promoter, promoter control elements, and combinations, and uses thereof
KR20010090954A (ko) * 2000-04-08 2001-10-22 우종일 수박의 종자 발생시 종피특이적으로 유전자의 발현을조절하는 Ｃｖ２０ｏｘ유전자 프로모터
CN102174540B (zh) * 2000-06-16 2013-08-07 托马斯·施穆林 改变植物形态学、生物化学和生理学的方法
US7294759B2 (en) * 2001-06-29 2007-11-13 E. I. Du Pont De Nemours And Company Alteration of oil traits in plants
AU2003220146A1 (en) * 2002-03-05 2003-09-22 Syngenta Participations Ag Nucleic acid sequences encoding the bos1 gene and promoter from arabidopisis thaliana and uses thereof
US20090288226A1 (en) * 2005-11-21 2009-11-19 Hallahan David L Method for manipulating growth, yield, and architecture in plants
PL2152885T3 (pl) * 2007-05-23 2014-11-28 Syngenta Participations Ag Markery polinukleotydowe
CN102597244B (zh) * 2009-10-26 2014-07-23 先锋国际良种公司 胚珠体细胞特异性启动子及应用方法
EP2800761A1 (en) * 2012-01-06 2014-11-12 Pioneer Hi-Bred International Inc. Methods and compositions for modulating expression or activity of a rkd polypeptide in a plant
BR112014016774A2 (pt) * 2012-01-06 2020-10-27 Pioneer Hi-Bred International, Inc método para produzir grande população de sementes, planta autorreprodutora e semente

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