BR112014014268B8 - Métodos para modular a condutância de estomas e estruturas de expressão de planta para execução da mesma - Google Patents

Métodos para modular a condutância de estomas e estruturas de expressão de planta para execução da mesma

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Publication number
BR112014014268B8
BR112014014268B8 BR112014014268A BR112014014268A BR112014014268B8 BR 112014014268 B8 BR112014014268 B8 BR 112014014268B8 BR 112014014268 A BR112014014268 A BR 112014014268A BR 112014014268 A BR112014014268 A BR 112014014268A BR 112014014268 B8 BR112014014268 B8 BR 112014014268B8
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BR
Brazil
Prior art keywords
methods
plant expression
same
modulate
perform
Prior art date
Application number
BR112014014268A
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English (en)
Other versions
BR112014014268A2 (pt
BR112014014268B1 (pt
Inventor
David Granot
Gilor Kelly
Menachem Moshelion
Original Assignee
The State Of Israel Miniistry Of Agriculture & Rural Development Agricultural Res Organization A R O
Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd
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Publication date
Application filed by The State Of Israel Miniistry Of Agriculture & Rural Development Agricultural Res Organization A R O, Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd filed Critical The State Of Israel Miniistry Of Agriculture & Rural Development Agricultural Res Organization A R O
Publication of BR112014014268A2 publication Critical patent/BR112014014268A2/pt
Publication of BR112014014268B1 publication Critical patent/BR112014014268B1/pt
Publication of BR112014014268B8 publication Critical patent/BR112014014268B8/pt

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02—Flowers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

métodos para modular a condutância de estomas e estruturas de expres-são de planta para execução da mesma, fornece uma estrutura de expressão de plan-tas; de acordo com uma aplicação, a estrutura de expressão de planta compreende uma sequên-cia de ácido nucleico que codifica uma hexoquinase sob um controle transcricional de um ele-mento regulador de ação cis especifico de células-guardas; também são fornecidos métodos para uso das estruturas e plantas transgênicas, células de plantas e partes de plantas que expressam as mesmas.
BR112014014268A 2011-12-11 2012-12-11 Métodos para modular a condutância de estomas e estruturas de expressão de planta para execução da mesma BR112014014268B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161569251P 2011-12-11 2011-12-11
US61/569,251 2011-12-11
PCT/IL2012/050519 WO2013088438A1 (en) 2011-12-11 2012-12-11 Methods of modulating stomata conductance and plant expression constructs for executing same

Publications (3)

Publication Number Publication Date
BR112014014268A2 BR112014014268A2 (pt) 2017-07-04
BR112014014268B1 BR112014014268B1 (pt) 2022-05-31
BR112014014268B8 true BR112014014268B8 (pt) 2022-06-14

Family

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BR112014014268A BR112014014268B8 (pt) 2011-12-11 2012-12-11 Métodos para modular a condutância de estomas e estruturas de expressão de planta para execução da mesma

Country Status (11)

Country Link
US (2) US20140345000A1 (pt)
EP (1) EP2788492B1 (pt)
CN (1) CN104379749B (pt)
AU (1) AU2012354054B2 (pt)
BR (1) BR112014014268B8 (pt)
CA (1) CA2860011C (pt)
ES (1) ES2651244T3 (pt)
MX (1) MX2014006966A (pt)
RU (1) RU2664461C2 (pt)
UA (1) UA116097C2 (pt)
WO (1) WO2013088438A1 (pt)

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CN106168822A (zh) * 2016-09-30 2016-11-30 深圳前海弘稼科技有限公司 一种温室环境调控方法、装置及智能温室
CN106770943B (zh) * 2016-12-12 2023-02-03 甘肃农业大学 马铃薯气孔导度测定仪及其测定方法
CN109735538B (zh) * 2018-12-27 2022-06-21 南京农业大学 一种提高森林草莓叶片再生效率的载体及其制备方法和应用
CN109554379B (zh) * 2018-12-30 2022-02-22 中国热带农业科学院热带生物技术研究所 一种甘蔗己糖激酶ShHXK8基因及其克隆方法和应用
NL1043218B1 (en) * 2019-04-03 2020-10-08 Lomasgrow Holding Bv Thermal image analyser for crops
CN110964735B (zh) * 2019-12-11 2021-06-18 浙江大学 水稻基因OsHXK9在调控种子休眠性中的应用
CN111955187B (zh) * 2020-05-29 2021-11-16 中国计量大学 利用相互嫁接体定量分析植物气孔关闭与根部基因型关系的方法
CN112391405B (zh) * 2020-11-17 2022-11-18 中国农业科学院茶叶研究所 茶树己糖激酶CsHXK3基因在调控植物生长发育和增强抗寒能力中的应用
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EP2788492A4 (en) 2015-07-15
RU2664461C2 (ru) 2018-08-17
US20190284569A1 (en) 2019-09-19
ES2651244T3 (es) 2018-01-25
CN104379749B (zh) 2018-08-07
CA2860011C (en) 2021-07-27
WO2013088438A1 (en) 2013-06-20
MX2014006966A (es) 2015-06-05
CA2860011A1 (en) 2013-06-20
AU2012354054A1 (en) 2014-07-31
RU2014128294A (ru) 2016-01-27
US10975385B2 (en) 2021-04-13
CN104379749A (zh) 2015-02-25
BR112014014268A2 (pt) 2017-07-04
EP2788492A1 (en) 2014-10-15
EP2788492B1 (en) 2017-09-06
US20140345000A1 (en) 2014-11-20
AU2012354054B2 (en) 2018-07-26
BR112014014268B1 (pt) 2022-05-31
UA116097C2 (uk) 2018-02-12

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