BR112013028522A2 - método e inserção para separação de gradiente de densidade - Google Patents
método e inserção para separação de gradiente de densidadeInfo
- Publication number
- BR112013028522A2 BR112013028522A2 BR112013028522A BR112013028522A BR112013028522A2 BR 112013028522 A2 BR112013028522 A2 BR 112013028522A2 BR 112013028522 A BR112013028522 A BR 112013028522A BR 112013028522 A BR112013028522 A BR 112013028522A BR 112013028522 A2 BR112013028522 A2 BR 112013028522A2
- Authority
- BR
- Brazil
- Prior art keywords
- tube
- insert
- density gradient
- gradient separation
- centrifuge tube
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03D—FLOTATION; DIFFERENTIAL SEDIMENTATION
- B03D3/00—Differential sedimentation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/08—Rotary bowls
- B04B7/12—Inserts, e.g. armouring plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0609—Holders integrated in container to position an object
- B01L2300/0618—Holders integrated in container to position an object for removable separation walls
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Sustainable Development (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Centrifugal Separators (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
método e inserção para separação de gradiente de densidade uma inserção para um tubo de centrifugação adequado para utilização na separação de gradiente de densidade é descrita. a inserção inclui um membro dimensionado para ajustar dentro do tubo para dividir o tubo em uma porção de topo e uma porção de fundo. opcionalmente, a inserção tem um suporte estendendo ou pendendo a partir do membro para posicionamento do membro no interior no tubo. pelo menos duas aberturas estão localizadas sobre o membro de modo que uma primeira abertura está mais perto de uma extremidade de fundo do tubo em relação a uma segunda abertura quando a inserção é posicionada no tubo de centrifugação. também são descritos métodos para a separação de uma população alvo de células a partir de uma amostra utilizando a inserção de um tubo de centrifugação.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161482886P | 2011-05-05 | 2011-05-05 | |
US61/482,886 | 2011-05-05 | ||
PCT/CA2012/000418 WO2012149641A1 (en) | 2011-05-05 | 2012-05-03 | Method and insert for density gradient separation |
Publications (3)
Publication Number | Publication Date |
---|---|
BR112013028522A2 true BR112013028522A2 (pt) | 2017-01-10 |
BR112013028522B1 BR112013028522B1 (pt) | 2020-07-21 |
BR112013028522B8 BR112013028522B8 (pt) | 2021-03-30 |
Family
ID=47107710
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112013028522A BR112013028522B8 (pt) | 2011-05-05 | 2012-05-03 | inserção para um tubo de centrifugação, tubo de centrifugação compreendendo a mesma, método para separação de uma população alvo de células de uma amostra e kit |
Country Status (7)
Country | Link |
---|---|
US (1) | US9683983B2 (pt) |
EP (1) | EP2704841B1 (pt) |
CN (1) | CN103619484B (pt) |
AU (1) | AU2012250459B2 (pt) |
BR (1) | BR112013028522B8 (pt) |
CA (1) | CA2872686C (pt) |
WO (1) | WO2012149641A1 (pt) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9533303B2 (en) | 2012-11-30 | 2017-01-03 | Rarecyte, Inc. | Apparatus, system, and method for collecting a target material |
US9039999B2 (en) | 2012-11-30 | 2015-05-26 | Rarecyte, Inc. | Apparatus, system, and method for collecting a target material |
US9539570B2 (en) | 2012-11-30 | 2017-01-10 | Rarecyte, Inc. | Apparatus, system, and method for collecting a target material |
US9956555B2 (en) | 2012-11-30 | 2018-05-01 | Rarecyte, Inc. | Apparatus, system, and method for collecting a target material |
US9945839B2 (en) | 2012-11-30 | 2018-04-17 | Rarecyte, Inc. | Apparatus, system, and method for collecting a target material |
US9513291B2 (en) | 2012-11-30 | 2016-12-06 | Rarecyte, Inc. | Apparatus, system, and method for collecting a target material |
US10054524B2 (en) | 2012-11-30 | 2018-08-21 | Rarecyte, Inc. | Apparatus, system and method for collecting a target material |
US9625360B2 (en) | 2012-11-30 | 2017-04-18 | Rarecyte, Inc. | Apparatus, system, and method for collecting a target material |
EP2934405B1 (en) * | 2012-12-21 | 2017-12-20 | Bostik, Inc. | Novel process for dosing super absorbent particles |
DE102013103153B4 (de) * | 2013-03-27 | 2016-09-08 | Andreas Hettich Gmbh & Co. Kg | Abstützungsvorrichtung für einen Zentrifugiergutbehälter |
EP3068304B1 (de) * | 2013-11-14 | 2017-08-09 | Greiner Bio-One GmbH | Aufnahmevorrichtung, verfahren zum bereitstellen derselben sowie verfahren zum trennen eines gemisches |
CN104031831B (zh) * | 2014-07-01 | 2019-10-15 | 北京圣浦博大生物科技有限公司 | 一种血细胞分离管和单个核细胞的分离方法 |
GB201418382D0 (en) * | 2014-10-16 | 2014-12-03 | Ge Healthcare Uk Ltd | Device and method for separating cells |
US20160223557A1 (en) * | 2015-02-04 | 2016-08-04 | Oregon Health & Science University | Method of isolating circulating tumor cells |
WO2017046736A1 (en) * | 2015-09-15 | 2017-03-23 | Spark-Tech Sp. Z O.O. | Device and method for fluids separation by density gradient |
PL237582B1 (pl) * | 2015-09-15 | 2021-05-04 | Spark Tech Spolka Z Ograniczona Odpowiedzialnoscia | Insert do pojemnika do wirowania, zwłaszcza, do probówki, do wydzielenia frakcji o pożądanym zakresie gęstości metodą wirowania na gradiencie gęstości oraz pojemnik do wirowania zawierający ten insert |
WO2017091565A1 (en) | 2015-11-24 | 2017-06-01 | Royal Biologics | Methods and apparatus for separating fluid components |
CN107398358B (zh) | 2016-05-19 | 2020-04-21 | 阿尔法韦士曼公司 | 具有部分通道的离心转子芯 |
WO2018139583A1 (ja) * | 2017-01-27 | 2018-08-02 | 公益財団法人神戸医療産業都市推進機構 | 単核球分離装置及び単核球分離方法 |
WO2018231960A1 (en) | 2017-06-13 | 2018-12-20 | Veterinary Diagnostics Institute, Inc. | System and procedure for stabilizing, storing and recovering blood samples |
CN109030146A (zh) * | 2018-07-25 | 2018-12-18 | 麦克奥迪(厦门)医疗诊断系统有限公司 | 一种细胞制片试剂、试剂盒及使用方法 |
US11604191B2 (en) * | 2018-08-31 | 2023-03-14 | Thermogenesis Corporation | Methods for isolating target cells from blood |
US11344880B2 (en) | 2018-11-30 | 2022-05-31 | Hans-Werner Heinrich | Centrifuge tube separation system, and methods of use |
CN110935570A (zh) * | 2019-11-07 | 2020-03-31 | 曾彩霞 | 一种轻质物质多级分离的倾斜生物离心机 |
CN111974475B (zh) * | 2020-08-20 | 2021-09-21 | 四川行之智汇知识产权运营有限公司 | 血液成分分离器 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5248480A (en) * | 1992-05-28 | 1993-09-28 | Diasys Corporation | Apparatus for drawing fluid sample and components thereof |
US5455009A (en) | 1993-09-14 | 1995-10-03 | Becton, Dickinson And Company | Blood collection assembly including clot-accelerating plastic insert |
US5840502A (en) * | 1994-08-31 | 1998-11-24 | Activated Cell Therapy, Inc. | Methods for enriching specific cell-types by density gradient centrifugation |
US5663051A (en) * | 1994-08-31 | 1997-09-02 | Activated Cell Therapy, Inc. | Separation apparatus and method |
CN101185918A (zh) * | 2007-12-12 | 2008-05-28 | 湖南大学 | 采用普通玻璃离心管实现固液高速离心分离的机构 |
US9333445B2 (en) * | 2008-07-21 | 2016-05-10 | Becton, Dickinson And Company | Density phase separation device |
-
2012
- 2012-05-03 WO PCT/CA2012/000418 patent/WO2012149641A1/en active Application Filing
- 2012-05-03 AU AU2012250459A patent/AU2012250459B2/en active Active
- 2012-05-03 CA CA2872686A patent/CA2872686C/en active Active
- 2012-05-03 US US14/115,446 patent/US9683983B2/en active Active
- 2012-05-03 EP EP12779315.6A patent/EP2704841B1/en active Active
- 2012-05-03 BR BR112013028522A patent/BR112013028522B8/pt active Search and Examination
- 2012-05-03 CN CN201280029951.0A patent/CN103619484B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
AU2012250459A1 (en) | 2013-05-02 |
BR112013028522B8 (pt) | 2021-03-30 |
CA2872686A1 (en) | 2012-11-08 |
EP2704841A1 (en) | 2014-03-12 |
EP2704841B1 (en) | 2017-02-01 |
CN103619484A (zh) | 2014-03-05 |
US20140087360A1 (en) | 2014-03-27 |
CN103619484B (zh) | 2018-03-09 |
WO2012149641A1 (en) | 2012-11-08 |
CA2872686C (en) | 2019-09-03 |
US9683983B2 (en) | 2017-06-20 |
AU2012250459B2 (en) | 2015-06-04 |
BR112013028522B1 (pt) | 2020-07-21 |
EP2704841A4 (en) | 2014-10-22 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B06F | Objections, documents and/or translations needed after an examination request according art. 34 industrial property law | ||
B06U | Preliminary requirement: requests with searches performed by other patent offices: suspension of the patent application procedure | ||
B09A | Decision: intention to grant | ||
B16A | Patent or certificate of addition of invention granted |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 03/05/2012, OBSERVADAS AS CONDICOES LEGAIS. |
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B09W | Decision of grant: rectification |
Free format text: REFERENTE A RPI 2574 DE 05/05/2020 |
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B16C | Correction of notification of the grant |
Free format text: REF. RPI 2585 DE 21/07/2020 QUANTO AO RD. |