BR112013027846B8 - container for in vitro culture of plant material, by temporary immersion - Google Patents

container for in vitro culture of plant material, by temporary immersion Download PDF

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Publication number
BR112013027846B8
BR112013027846B8 BR112013027846A BR112013027846A BR112013027846B8 BR 112013027846 B8 BR112013027846 B8 BR 112013027846B8 BR 112013027846 A BR112013027846 A BR 112013027846A BR 112013027846 A BR112013027846 A BR 112013027846A BR 112013027846 B8 BR112013027846 B8 BR 112013027846B8
Authority
BR
Brazil
Prior art keywords
plant material
container
vitro culture
upper chamber
temporary immersion
Prior art date
Application number
BR112013027846A
Other languages
Portuguese (pt)
Other versions
BR112013027846B1 (en
BR112013027846A2 (en
Inventor
Bertrand Benoît
Georget Frédéric
Etienne Hervé
Original Assignee
Centre De Cooperation Internationale En Rech Agronomique Pour Le Developpement C I R A D
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre De Cooperation Internationale En Rech Agronomique Pour Le Developpement C I R A D filed Critical Centre De Cooperation Internationale En Rech Agronomique Pour Le Developpement C I R A D
Publication of BR112013027846A2 publication Critical patent/BR112013027846A2/en
Publication of BR112013027846B1 publication Critical patent/BR112013027846B1/en
Publication of BR112013027846B8 publication Critical patent/BR112013027846B8/en

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002Culture media for tissue culture

Abstract

recipiente para a cultura in vitro de material vegetal, por imersão temporária a presente invenção se refere a um recipiente para a cultura in vitro de material vegetal, por imersão temporária, recipiente (1) esse que compreende, por um lado, uma câmara superior (3)m destinada a conter esse material vegetal (m) a cultivar, e uma câmara inferior (4)m destinada a conter um líquido nutritivo (l), assim como um meio (5) para transferir temporariamente pelo menos uma parte desse líquido nutritivo (l) desse câmara inferior (4) para essa câmara superior (3). de acordo com a invenção, a face inferior (3c) da câmara superior (3)m destinada a sustentar esse material vegetal, apresenta uma superfície horizontal (s) compreendida entre 0,02 e 0,07 m2, e a face lateral (3a) da câmara superior (3) apresenta uma altura (h) compreendida entre 40 e 130 mm. essa combinação de características estruturais permite melhorar o acesso à luz e a atividade fotossintética da biomassa, assim como evitar os fenômenos de compactação e de estratificação durante a cultura do material vegetal, para otimizar o desenvolvimento do material vegetal.container for the in vitro culture of plant material, by temporary immersion the present invention relates to a container for the in vitro culture of plant material, by temporary immersion, container (1) which comprises, on the one hand, an upper chamber ( 3) m designed to contain this plant material (m) to be cultivated, and an lower chamber (4) m designed to contain a nutritive liquid (l), as well as a means (5) to temporarily transfer at least a part of that nutrient liquid (l) from that lower chamber (4) to that upper chamber (3). according to the invention, the lower face (3c) of the upper chamber (3) m, intended to support this plant material, has a horizontal surface (s) between 0.02 and 0.07 m2, and the lateral face (3a ) of the upper chamber (3) has a height (h) between 40 and 130 mm. this combination of structural characteristics allows to improve the access to light and the photosynthetic activity of biomass, as well as to avoid the phenomena of compaction and stratification during the cultivation of plant material, to optimize the development of plant material.

BR112013027846A 2011-04-28 2012-04-25 container for in vitro culture of plant material, by temporary immersion BR112013027846B8 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1153642A FR2974580B1 (en) 2011-04-28 2011-04-28 CONTAINER FOR IN VITRO CULTIVATION OF PLANT MATERIAL IN STERILE CONDITIONS BY TEMPORARY IMMERSION
PCT/FR2012/050919 WO2012146872A1 (en) 2011-04-28 2012-04-25 Container for in vitro cultivation of plant material, by temporary immersion

Publications (3)

Publication Number Publication Date
BR112013027846A2 BR112013027846A2 (en) 2016-08-09
BR112013027846B1 BR112013027846B1 (en) 2020-05-05
BR112013027846B8 true BR112013027846B8 (en) 2020-06-02

Family

ID=46201679

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013027846A BR112013027846B8 (en) 2011-04-28 2012-04-25 container for in vitro culture of plant material, by temporary immersion

Country Status (5)

Country Link
AR (1) AR086142A1 (en)
BR (1) BR112013027846B8 (en)
FR (1) FR2974580B1 (en)
MX (1) MX357396B (en)
WO (1) WO2012146872A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104328049B (en) * 2014-11-07 2016-04-20 天津迈安诊生物技术有限公司 Eyeball leading portion perfused organ cultivates multichamber system
GB2533129A (en) * 2014-12-11 2016-06-15 Alkion Biopharma Sas Bioreactor
CN104663438A (en) * 2015-02-09 2015-06-03 南京邦林生物科技有限公司 Tide-type plant tissue culture device and systematic culture device
JP2017118847A (en) 2015-12-28 2017-07-06 キリン株式会社 Mass production method of plants, and mass production equipment, and culture bags used therefor
CN110558000B (en) * 2019-09-30 2021-06-29 马鞍山市全润农业科技有限公司 Greenhouse high-yield pepper cultivation and seed selection device and method
US11766002B2 (en) 2019-09-30 2023-09-26 Alkion Bioinnovations Method for production of sterile flowering biomass in temporary immersion bioreactors
FR3103078A1 (en) 2019-11-18 2021-05-21 Cid Plastiques Device for plant culture in vitro by temporary immersion in a nutrient liquid
WO2023175125A1 (en) 2022-03-18 2023-09-21 Alkion Bioinnovations Biological method for decarboxylation of cannabinoids directly in plant tissues

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2730743B1 (en) * 1995-02-17 1997-05-09 Cooperation Internationale En IN VITRO CULTURE CONTAINER
TWI323641B (en) * 2006-12-29 2010-04-21 Tatung Co Tissues with rich flavonoids of neomarica gracilis and belamcanda chinensis and culture methods for the same
US20100083573A1 (en) * 2008-10-07 2010-04-08 Steven William Gallo System for growing plants

Also Published As

Publication number Publication date
FR2974580A1 (en) 2012-11-02
FR2974580B1 (en) 2014-01-24
WO2012146872A1 (en) 2012-11-01
BR112013027846B1 (en) 2020-05-05
MX2013012473A (en) 2014-08-22
AR086142A1 (en) 2013-11-20
MX357396B (en) 2018-07-06
BR112013027846A2 (en) 2016-08-09

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 25/04/2012, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: REF. RPI 2574 DE 05/05/2020 QUANTO AO TITULAR.