BR112013027846A2 - container for in vitro culture of plant material by temporary immersion - Google Patents

container for in vitro culture of plant material by temporary immersion

Info

Publication number
BR112013027846A2
BR112013027846A2 BR112013027846A BR112013027846A BR112013027846A2 BR 112013027846 A2 BR112013027846 A2 BR 112013027846A2 BR 112013027846 A BR112013027846 A BR 112013027846A BR 112013027846 A BR112013027846 A BR 112013027846A BR 112013027846 A2 BR112013027846 A2 BR 112013027846A2
Authority
BR
Brazil
Prior art keywords
plant material
container
vitro culture
upper chamber
temporary
Prior art date
Application number
BR112013027846A
Other languages
Portuguese (pt)
Other versions
BR112013027846B1 (en
BR112013027846B8 (en
Inventor
Benoît Bertrand
Frédéric Georget
Hervé Etienne
Original Assignee
Cirad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cirad filed Critical Cirad
Publication of BR112013027846A2 publication Critical patent/BR112013027846A2/en
Publication of BR112013027846B1 publication Critical patent/BR112013027846B1/en
Publication of BR112013027846B8 publication Critical patent/BR112013027846B8/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002—Culture media for tissue culture

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Hydroponics (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

recipiente para a cultura in vitro de material vegetal, por imersão temporária a presente invenção se refere a um recipiente para a cultura in vitro de material vegetal, por imersão temporária, recipiente (1) esse que compreende, por um lado, uma câmara superior (3)m destinada a conter esse material vegetal (m) a cultivar, e uma câmara inferior (4)m destinada a conter um líquido nutritivo (l), assim como um meio (5) para transferir temporariamente pelo menos uma parte desse líquido nutritivo (l) desse câmara inferior (4) para essa câmara superior (3). de acordo com a invenção, a face inferior (3c) da câmara superior (3)m destinada a sustentar esse material vegetal, apresenta uma superfície horizontal (s) compreendida entre 0,02 e 0,07 m2, e a face lateral (3a) da câmara superior (3) apresenta uma altura (h) compreendida entre 40 e 130 mm. essa combinação de características estruturais permite melhorar o acesso à luz e a atividade fotossintética da biomassa, assim como evitar os fenômenos de compactação e de estratificação durante a cultura do material vegetal, para otimizar o desenvolvimento do material vegetal.Container for in vitro culture of plant material by temporary dipping The present invention relates to a container for in vitro culture of plant material by temporary dipping, which container (1) comprises, on the one hand, an upper chamber ( 3) m for containing such plant material (m) for cultivation, and a lower chamber (4) m for containing a nutritive liquid (1) as well as a means (5) for temporarily transferring at least a portion of that nutritive liquid (l) from that lower chamber (4) to that upper chamber (3). According to the invention, the lower face (3c) of the upper chamber (3) for supporting such plant material has a horizontal surface (s) of between 0.02 and 0.07 m2 and the side face (3a). ) of the upper chamber (3) has a height (h) of between 40 and 130 mm. This combination of structural features allows for improved light access and photosynthetic activity of biomass, as well as avoiding compaction and stratification phenomena during plant material culture to optimize plant material development.

BR112013027846A 2011-04-28 2012-04-25 container for in vitro culture of plant material, by temporary immersion BR112013027846B8 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1153642A FR2974580B1 (en) 2011-04-28 2011-04-28 CONTAINER FOR IN VITRO CULTIVATION OF PLANT MATERIAL IN STERILE CONDITIONS BY TEMPORARY IMMERSION
PCT/FR2012/050919 WO2012146872A1 (en) 2011-04-28 2012-04-25 Container for in vitro cultivation of plant material, by temporary immersion

Publications (3)

Publication Number Publication Date
BR112013027846A2 true BR112013027846A2 (en) 2016-08-09
BR112013027846B1 BR112013027846B1 (en) 2020-05-05
BR112013027846B8 BR112013027846B8 (en) 2020-06-02

Family

ID=46201679

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013027846A BR112013027846B8 (en) 2011-04-28 2012-04-25 container for in vitro culture of plant material, by temporary immersion

Country Status (5)

Country Link
AR (1) AR086142A1 (en)
BR (1) BR112013027846B8 (en)
FR (1) FR2974580B1 (en)
MX (1) MX357396B (en)
WO (1) WO2012146872A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104328049B (en) * 2014-11-07 2016-04-20 天津迈安诊生物技术有限公司 Eyeball leading portion perfused organ cultivates multichamber system
GB2533129A (en) 2014-12-11 2016-06-15 Alkion Biopharma Sas Bioreactor
CN104663438A (en) * 2015-02-09 2015-06-03 南京邦林生物科技有限公司 Tide-type plant tissue culture device and systematic culture device
JP2017118847A (en) 2015-12-28 2017-07-06 キリン株式会社 Mass production method of plants, and mass production equipment, and culture bags used therefor
CN110558000B (en) * 2019-09-30 2021-06-29 马鞍山市全润农业科技有限公司 Greenhouse high-yield pepper cultivation and seed selection device and method
WO2021063996A1 (en) 2019-09-30 2021-04-08 Alkion Bioinnovations Method for production of sterile flowering biomass in temporary immersion bioreactors
FR3103078A1 (en) 2019-11-18 2021-05-21 Cid Plastiques Device for plant culture in vitro by temporary immersion in a nutrient liquid
WO2023175125A1 (en) 2022-03-18 2023-09-21 Alkion Bioinnovations Biological method for decarboxylation of cannabinoids directly in plant tissues

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2730743B1 (en) * 1995-02-17 1997-05-09 Cooperation Internationale En IN VITRO CULTURE CONTAINER
TWI323641B (en) * 2006-12-29 2010-04-21 Tatung Co Tissues with rich flavonoids of neomarica gracilis and belamcanda chinensis and culture methods for the same
US20100083573A1 (en) * 2008-10-07 2010-04-08 Steven William Gallo System for growing plants

Also Published As

Publication number Publication date
BR112013027846B1 (en) 2020-05-05
BR112013027846B8 (en) 2020-06-02
FR2974580B1 (en) 2014-01-24
AR086142A1 (en) 2013-11-20
MX2013012473A (en) 2014-08-22
MX357396B (en) 2018-07-06
WO2012146872A1 (en) 2012-11-01
FR2974580A1 (en) 2012-11-02

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 25/04/2012, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: REF. RPI 2574 DE 05/05/2020 QUANTO AO TITULAR.