BR112013016876A2 - métodos e composições para um sistema de espressão transiente de soja in-planta - Google Patents

métodos e composições para um sistema de espressão transiente de soja in-planta

Info

Publication number
BR112013016876A2
BR112013016876A2 BR112013016876A BR112013016876A BR112013016876A2 BR 112013016876 A2 BR112013016876 A2 BR 112013016876A2 BR 112013016876 A BR112013016876 A BR 112013016876A BR 112013016876 A BR112013016876 A BR 112013016876A BR 112013016876 A2 BR112013016876 A2 BR 112013016876A2
Authority
BR
Brazil
Prior art keywords
plant
methods
soybean
espression
compositions
Prior art date
Application number
BR112013016876A
Other languages
English (en)
Other versions
BR112013016876B1 (pt
Inventor
Kasimalai Azhanandam
Pei Su
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of BR112013016876A2 publication Critical patent/BR112013016876A2/pt
Publication of BR112013016876B1 publication Critical patent/BR112013016876B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

métodos e composições para uma sistema de espressão transiente de soja in-planta a presente invenção refere-se a métodos para expressar transientemente uma sequência de nucleotídeos em uma ou mais células de uma planta de soja, compreendendo: a) abrasar a superfície de uma planta de soja e/ou parte intata dessa; e ainda (b1) imergir a uma planta abrasada e/ou e ou a parte dessa abrasada em uma solução compreendendo células de agrobacterium e um surfatante, em que as células de agrobacterium compreendem a sequência de nucleotídeos a ser expressa transientemente; (b2) aplicar um vácuo à planta de soja e/ou parte intata imersa do passo (b1); e (b3) libertar o vácuo, introduzindo desse modo a solução na planta de soja e/ou na parte intata dessa; ou (c) contatar a planta de soja abrasada e/ou parte dessa abrasada com uma solução compreendendo células de agrobacterium e um surfatante, em que as células de agrobacterium compreendem a sequência de nucleotídeos a ser expressa transientemente, através da qual a sequência de nucleotídeos é expressa transientemente em uma ou mais células da planta de soja.
BR112013016876A 2010-12-29 2011-12-15 método para expressar transientemente uma ou mais sequências de nucleotídeos em uma ou mais células de uma planta inteira intacta de soja BR112013016876B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201061427935P 2010-12-29 2010-12-29
PCT/US2011/065092 WO2012091939A1 (en) 2010-12-29 2011-12-15 Methods and compositions for a soybean in-planta transient expression system

Publications (2)

Publication Number Publication Date
BR112013016876A2 true BR112013016876A2 (pt) 2016-10-25
BR112013016876B1 BR112013016876B1 (pt) 2020-06-09

Family

ID=46382060

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013016876A BR112013016876B1 (pt) 2010-12-29 2011-12-15 método para expressar transientemente uma ou mais sequências de nucleotídeos em uma ou mais células de uma planta inteira intacta de soja

Country Status (6)

Country Link
US (1) US9068191B2 (pt)
CN (1) CN103298336A (pt)
AU (1) AU2011352873C1 (pt)
BR (1) BR112013016876B1 (pt)
CA (1) CA2823237C (pt)
WO (1) WO2012091939A1 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
EP2755466A4 (en) 2011-09-13 2015-04-15 Monsanto Technology Llc METHODS AND COMPOSITIONS FOR CONTROLLING WEEDS
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
CN104619843B (zh) 2012-05-24 2020-03-06 A.B.种子有限公司 用于使基因表达沉默的组合物和方法
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
WO2014164761A1 (en) 2013-03-13 2014-10-09 Monsanto Technology Llc Methods and compositions for weed control
MX2015012334A (es) 2013-03-13 2016-02-05 Monsanto Technology Llc Metodos y composiciones para el control de malezas.
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
CA2918387C (en) 2013-07-19 2021-11-02 Monsanto Technology Llc Compositions and methods for controlling leptinotarsa
NZ719544A (en) 2013-11-04 2022-09-30 Beeologics Inc Compositions and methods for controlling arthropod parasite and pest infestations
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
AU2015280252A1 (en) 2014-06-23 2017-01-12 Monsanto Technology Llc Compositions and methods for regulating gene expression via RNA interference
EP3161138A4 (en) 2014-06-25 2017-12-06 Monsanto Technology LLC Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
CA2955842A1 (en) 2014-07-29 2016-02-04 Monsanto Technology Llc Compositions and methods for controlling insect pests
CN108064288B (zh) 2015-01-22 2021-11-26 孟山都技术公司 用于控制叶甲属的组合物和方法
CN107750125A (zh) 2015-06-02 2018-03-02 孟山都技术有限公司 用于将多核苷酸递送至植物中的组合物和方法
WO2016196782A1 (en) 2015-06-03 2016-12-08 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
US20230235344A1 (en) * 2020-06-24 2023-07-27 Benson Hill, Inc. Plant cell treatments to improve plant transformation
CN116648514A (zh) * 2020-12-22 2023-08-25 先正达农作物保护股份公司 玉米调节元件及其用途

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5276268A (en) 1986-08-23 1994-01-04 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
CA2267014A1 (en) 1999-03-26 2000-09-26 Brenda Rojas Monocot transformation using acrobacterium
US6573428B1 (en) 1999-10-20 2003-06-03 Board Of Trustees Of The University Of Illinois Soybean promoter expressed preferentially in pods
AU1803601A (en) 1999-11-29 2001-06-04 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
DE602005013775D1 (de) * 2004-06-07 2009-05-20 Basf Plant Science Gmbh Verbesserte methode zur transformation von sojabohnen
US7723570B2 (en) * 2004-10-12 2010-05-25 Soymeds, Inc. Edible vaccines expressed in soybeans

Also Published As

Publication number Publication date
BR112013016876B1 (pt) 2020-06-09
US20120174262A1 (en) 2012-07-05
AU2011352873A1 (en) 2013-07-25
CA2823237A1 (en) 2012-07-05
CN103298336A (zh) 2013-09-11
US9068191B2 (en) 2015-06-30
WO2012091939A1 (en) 2012-07-05
AU2011352873C1 (en) 2016-04-21
AU2011352873B2 (en) 2015-09-17
CA2823237C (en) 2020-12-29

Similar Documents

Publication Publication Date Title
BR112013016876A2 (pt) métodos e composições para um sistema de espressão transiente de soja in-planta
BR112017024649A2 (pt) métodos para a transformação in planta de plantas e processos de fabricação e produtos com base nelas e obteníveis a partir delas
CL2014002018A1 (es) Molécula de ácido nucleico que comprende una secuencia sintética variante de la enzima 5-enolpiruvil shikimato-3-fosfato sintasa para expresión en una planta; vector; célula huesped; método para producir una planta resistente al glifosato y métodos asociados.
AR090575A1 (es) Agrobacterium para la transfeccion transitoria de plantas completas
CL2013001115A1 (es) Polipeptido que presenta actividad enzimatica hidroxifenil piruvato dioxigenasa (hppd); polinucleotido que codifica dicho polipeptido; casete de expresión, vector que comprende el casete; celula vegetal transformada con dicho vector; metodo para conferir resistencia o tolerancia a un inhibidor de hppd en una planta
BR112013020384B8 (pt) Método de revestimento de um substrato fertilizante
CR20120254A (es) Células madre multipotenciales en el árbol de billary extrahepática y métodos de aislamiento del mismo
CL2016000902A1 (es) Molécula de ácido nucleico aislada que codifica una enzima delta-9-desaturasa, método para generar plantas que comprenden dicho ácido nucleico, y procedimiento para disminuir la cantidad de ácidos grasos saturados en una planta. (divisional de sol. n°3665-12).
BRPI0806831B8 (pt) métodos atuadores de gotículas empregando esferas magnéticas
AR080742A1 (es) Peptido gamma-zeina vegetal para la administracion de biomoleculas en celulas vegetales
BR112018014026A2 (pt) composição líquida compreendendo um polímero múltiplas fases, seu método de preparação e uso
BR112017017279A2 (pt) métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta
MX357280B (es) Método sin alimentadoras para cultivo de células madre espermatogonias de bovino y porcino.
BR112016006637A2 (pt) método para introduzir uma substância em uma planta, e, planta
BR112018014029A2 (pt) composição líquida compreendendo um polímero múltiplas fases e um monômero (met) acrílico, seu método de preparação e uso
BR112012029644A2 (pt) composições de decapagem e métodos de realizar e usar os mesmos
BR112017023722A2 (pt) composição formadora de película curável, substrato revestido e método para revestir um substrato
BR112015008189A2 (pt) composição, processo para sua preparação e compósito
BR112014032779A2 (pt) processo de separação de materiais renováveis a partir de micro-organismos
UY36909A (es) Promotor y 3’ utr de plantas para la expresión del transgén
BR112014019936A8 (pt) Composição e método para colar dois ou mais substratos
BR112015024728A2 (pt) processo de fabricação de um confeito congelado
CL2009001935A1 (es) Vector que comprende una molécula de ácido nucleico capaz de dirigir la expresión génica en el desarrollo de semillas; célula que lo contiene; método para transformar una célula con dicho vector, y procedimiento para identificar alelos fad3a y fad3b en una planta de lino (div. sol. n°267-06).
MX2016017322A (es) Metodo para la union de sustratos a superficies frias y humedas.
BR112015001616B8 (pt) Método para aumento do teor de sus em um óleo ou em uma fração de oleína por intraesterificação 1,3-específica, óleo ou oleína intraesterificado 1,3-seletivo e seu uso

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B06T Formal requirements before examination
B07A Technical examination (opinion): publication of technical examination (opinion)
B09A Decision: intention to grant
B16A Patent or certificate of addition of invention granted

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 15/12/2011, OBSERVADAS AS CONDICOES LEGAIS.