BR112013003528A2 - ''solução para extrair rna e métodos para extrair rna'' - Google Patents

''solução para extrair rna e métodos para extrair rna''

Info

Publication number
BR112013003528A2
BR112013003528A2 BR112013003528A BR112013003528A BR112013003528A2 BR 112013003528 A2 BR112013003528 A2 BR 112013003528A2 BR 112013003528 A BR112013003528 A BR 112013003528A BR 112013003528 A BR112013003528 A BR 112013003528A BR 112013003528 A2 BR112013003528 A2 BR 112013003528A2
Authority
BR
Brazil
Prior art keywords
rna extraction
methods
solution
extraction solution
extraction methods
Prior art date
Application number
BR112013003528A
Other languages
English (en)
Other versions
BR112013003528B1 (pt
Inventor
Hideo Akiyama
Hitoshi Nobumasa
Makiko Yoshimoto
Original Assignee
Toray Industries
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries filed Critical Toray Industries
Publication of BR112013003528A2 publication Critical patent/BR112013003528A2/pt
Publication of BR112013003528B1 publication Critical patent/BR112013003528B1/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C01INORGANIC CHEMISTRY
    • C01CAMMONIA; CYANOGEN; COMPOUNDS THEREOF
    • C01C3/00Cyanogen; Compounds thereof
    • C01C3/20Thiocyanic acid; Salts thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C39/00Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a six-membered aromatic ring
    • C07C39/02Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a six-membered aromatic ring monocyclic with no unsaturation outside the aromatic ring
    • C07C39/04Phenol
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866Grinding or homogeneising

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
BR112013003528-5A 2010-08-18 2011-08-17 Solução para extrair rna e métodos para extrair rna BR112013003528B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2010183280 2010-08-18
JP2010-183280 2010-08-18
PCT/JP2011/068620 WO2012023569A1 (ja) 2010-08-18 2011-08-17 Rna抽出用溶液

Publications (2)

Publication Number Publication Date
BR112013003528A2 true BR112013003528A2 (pt) 2016-06-28
BR112013003528B1 BR112013003528B1 (pt) 2019-09-24

Family

ID=45605222

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013003528-5A BR112013003528B1 (pt) 2010-08-18 2011-08-17 Solução para extrair rna e métodos para extrair rna

Country Status (13)

Country Link
US (3) US20130144049A1 (pt)
EP (1) EP2607482B1 (pt)
JP (1) JP5906740B2 (pt)
KR (1) KR101641113B1 (pt)
CN (1) CN103068979B (pt)
AU (1) AU2011291680B2 (pt)
BR (1) BR112013003528B1 (pt)
CA (1) CA2808265C (pt)
DK (1) DK2607482T3 (pt)
ES (1) ES2622964T3 (pt)
PL (1) PL2607482T3 (pt)
RU (1) RU2013111745A (pt)
WO (1) WO2012023569A1 (pt)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2561425B (en) 2012-03-16 2019-01-16 Cambridge Entpr Ltd Apparatus for obtaining liquid from a solid phase
WO2015022410A1 (en) * 2013-08-16 2015-02-19 General Electric Company Methods and compositions for extraction and storage of nucleic acids
CN103952399A (zh) * 2014-04-18 2014-07-30 天根生化科技(北京)有限公司 一种使用含有指示剂的酚胍盐裂解液提取核糖核酸的方法
JP6571679B2 (ja) 2014-04-25 2019-09-04 トランスレイト バイオ, インコーポレイテッド メッセンジャーrnaの精製方法
US9783799B2 (en) 2014-10-24 2017-10-10 Abbott Molecular Inc. Enrichment of small nucleic acids
KR20190014204A (ko) 2017-07-28 2019-02-12 가톨릭대학교 산학협력단 미세관을 이용한 rna 추출 방법
CN108587029B (zh) 2018-03-28 2020-09-08 中国石油天然气股份有限公司 一种相变材料液及其所形成的固相支撑剂
CN108676791B (zh) * 2018-04-25 2022-03-25 浙江迪恩生物科技股份有限公司 一种磁珠法提取dna的试剂盒及提取方法
CN110887970B (zh) * 2019-11-29 2023-10-31 北京赛科希德科技股份有限公司 抽提缓冲液、兔脑抽提液、pt检测试剂及pt检测试剂盒
KR102587121B1 (ko) 2020-09-15 2023-10-11 주식회사 제노헬릭스 Rna 분리용 조성물
KR102657767B1 (ko) 2021-12-02 2024-04-16 한화오션 주식회사 액화가스 연료 선박의 시운전용 연료 공급 시스템
CN114350650A (zh) * 2021-12-16 2022-04-15 力因精准医疗产品(上海)有限公司 无dna残留血液rna提取试剂盒及核酸提取方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB190600753A (en) * 1906-01-11 1906-12-06 William Phillips Thompson Improved Process and Apparatus for Distilling Coals and other Hydrocarbonaceous Substances.
JPS5344886B2 (pt) 1973-11-19 1978-12-02
US4843155A (en) 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
EP0755401B1 (en) * 1994-04-14 2002-09-18 The Rockefeller University Apparatus for isolating cellular components
US5945515A (en) * 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
AU6959998A (en) 1997-04-10 1998-10-30 Life Technologies, Inc. Rna isolation reagent and methods
US7794932B2 (en) 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
US7749519B2 (en) * 2005-12-09 2010-07-06 Kim Lee Sim Unique DNA and polypeptide sequences based on the circumsporozoite protein of Plasmodium vivax
WO2008157316A1 (en) * 2007-06-14 2008-12-24 Creighton University Improved method for rna isolation
US20110195030A1 (en) * 2008-10-15 2011-08-11 The University Of North Carolina At Chapel Hill Nanoparticle compositions comprising liquid oil cores

Also Published As

Publication number Publication date
BR112013003528B1 (pt) 2019-09-24
CA2808265C (en) 2016-09-06
EP2607482A4 (en) 2014-01-15
EP2607482A1 (en) 2013-06-26
CA2808265A1 (en) 2012-02-18
KR101641113B1 (ko) 2016-07-20
CN103068979B (zh) 2015-03-11
JPWO2012023569A1 (ja) 2013-10-28
AU2011291680B2 (en) 2014-01-09
US20170247681A1 (en) 2017-08-31
EP2607482B1 (en) 2017-04-05
DK2607482T3 (en) 2017-07-03
ES2622964T3 (es) 2017-07-10
US11851646B2 (en) 2023-12-26
US20130144049A1 (en) 2013-06-06
RU2013111745A (ru) 2014-09-27
WO2012023569A1 (ja) 2012-02-23
JP5906740B2 (ja) 2016-04-20
CN103068979A (zh) 2013-04-24
AU2011291680A1 (en) 2013-02-28
KR20130098163A (ko) 2013-09-04
PL2607482T3 (pl) 2017-09-29
US10647978B2 (en) 2020-05-12
US20180340166A1 (en) 2018-11-29

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]