BR112012029006A2 - métodos de ensaio de quimioluminescência homogêneos com sensibilidade aumentada - Google Patents

métodos de ensaio de quimioluminescência homogêneos com sensibilidade aumentada

Info

Publication number
BR112012029006A2
BR112012029006A2 BR112012029006A BR112012029006A BR112012029006A2 BR 112012029006 A2 BR112012029006 A2 BR 112012029006A2 BR 112012029006 A BR112012029006 A BR 112012029006A BR 112012029006 A BR112012029006 A BR 112012029006A BR 112012029006 A2 BR112012029006 A2 BR 112012029006A2
Authority
BR
Brazil
Prior art keywords
analyte
orbiting
chemiluminescent compound
oxygen
increased sensitivity
Prior art date
Application number
BR112012029006A
Other languages
English (en)
Inventor
Hashem Akhavan-Tafti
Jeff Todtleben
Michael Salvati
Nir Shapir
Richard S Handley
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Publication of BR112012029006A2 publication Critical patent/BR112012029006A2/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

métodos de ensaio de quimioluminescência homogêneos com sensibilidade aumentada. a presente invenção refere-se aos métodos para determinar um análito em um meio suspeito de conter no análito. um método compreende o tratamento de um meio suspeito de conter um análito sob condições tais que o análito, se estiver presente, faz com que um agente de fotossensibilização e um composto quimioluminescente fiquem bastante próximos um do outro. o agente de fotossensibilização gera o oxigênio de órbita simples e ativa o composto quimioluminescente quando este encontra-se bastante próximo. o sinal não específico gerado pelo oxigênio de órbita simples que não encontra-se próximo é reduzido ou suprimido ao usar um removedor de oxigênio de órbita simples (soq). o composto quimioluminescente ativado produz subsequentemente a luz. a quantidade de luz produzida está relacionada à quantidade de análito no meio. o uso de agentes de modulação de ruído melhora de maneira significativa as relações entre sinal e ruído e a sensibilidade ensaio. composições e kits também são apresentados.
BR112012029006A 2010-05-14 2011-05-13 métodos de ensaio de quimioluminescência homogêneos com sensibilidade aumentada BR112012029006A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33485010P 2010-05-14 2010-05-14
PCT/US2011/036509 WO2011143606A1 (en) 2010-05-14 2011-05-13 Homogeneous chemiluminescence assay methods with increased sensitivity

Publications (1)

Publication Number Publication Date
BR112012029006A2 true BR112012029006A2 (pt) 2016-07-26

Family

ID=44914735

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012029006A BR112012029006A2 (pt) 2010-05-14 2011-05-13 métodos de ensaio de quimioluminescência homogêneos com sensibilidade aumentada

Country Status (9)

Country Link
US (1) US20130084652A1 (pt)
EP (1) EP2569442A4 (pt)
JP (1) JP2013532275A (pt)
KR (1) KR20130091644A (pt)
CN (1) CN102892896A (pt)
AU (1) AU2011252833A1 (pt)
BR (1) BR112012029006A2 (pt)
SG (1) SG185409A1 (pt)
WO (1) WO2011143606A1 (pt)

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HUE031737T2 (en) * 2009-02-27 2017-07-28 Beckman Coulter Inc Solution phase homogeneous tests
WO2014152165A1 (en) 2013-03-15 2014-09-25 Siemens Healthcare Diagnostics Inc. Luminescent oxygen channeling immunoassays utilizing electrochemical discharge of singlet oxygen and methods of production and use thereof
ES2822663T3 (es) * 2013-03-15 2021-05-04 Siemens Healthcare Diagnostics Inc Inmunoensayos de canalización de oxígeno luminiscentes heterogéneos
US20140308751A1 (en) * 2013-04-12 2014-10-16 Siemens Healthcare Diagnostics Inc. Assays for Analyte Homologs
CN104833798B (zh) * 2015-04-27 2016-08-24 杭州金溪生物技术有限公司 基于均相化学发光技术的快速诊断试纸条
US11740238B2 (en) 2015-09-04 2023-08-29 Pioneer Hi-Bred International, Inc. Compositions and methods of detecting a substance of interest
US11047854B2 (en) * 2017-02-06 2021-06-29 Abbott Japan Llc Methods for reducing noise in signal-generating digital assays
FR3081321B1 (fr) * 2018-05-24 2020-06-12 Imv Technologies Dispositif de collecte de semence animale
US11320424B2 (en) 2018-07-13 2022-05-03 3M Innovative Properties Company Specific binding chemiluminescent assay
CN116539597A (zh) * 2018-07-18 2023-08-04 科美博阳诊断技术(上海)有限公司 一种cTnI均相化学发光检测试剂盒、检测方法与装置
JP7153493B2 (ja) * 2018-07-27 2022-10-14 シスメックス株式会社 生体粒子の測定方法、非特異シグナルの検出方法、生体粒子測定方法及び生体粒子を検出するための試薬キット
CN111122843A (zh) * 2018-10-31 2020-05-08 博阳生物科技（上海）有限公司 一种均相化学发光检测试剂盒
EP3877753A4 (en) * 2018-11-07 2022-10-26 University of Houston System NON-ENZYMATIC GLOW TESTS
CN112888945B (zh) * 2018-12-06 2022-07-12 武汉全景生物技术有限公司 免疫检测中消除阿霉素干扰的方法及免疫检测试剂盒
WO2020113529A1 (zh) * 2018-12-06 2020-06-11 深圳迈瑞生物医疗电子股份有限公司 免疫检测中消除茶碱干扰的方法及免疫检测试剂盒
CN109655615A (zh) * 2018-12-21 2019-04-19 广州市进德生物科技有限公司 一种完全均相测定脂蛋白相关磷脂酶a2的检测试剂盒及其方法
CN112007620B (zh) * 2019-05-30 2023-08-01 苏州海狸生物医学工程有限公司 一种链霉亲和素磁性微球的制备方法
GB201909027D0 (en) * 2019-06-24 2019-08-07 Psyros Diagnostics Ltd A method for detecting an analyte
CN116859057A (zh) * 2019-12-31 2023-10-10 科美诊断技术(苏州)有限公司 一种肌红蛋白的均相检测试剂盒及其应用
CN116400074A (zh) * 2019-12-31 2023-07-07 科美博阳诊断技术(上海)有限公司 一种crp检测试剂盒及其应用
CN111735942A (zh) * 2020-03-03 2020-10-02 浙江卓运生物科技有限公司 一种均相法化学发光检测方法
WO2021217178A1 (en) * 2020-04-24 2021-10-28 Siemens Healthcare Diagnostics Inc. Calibration and quality control reagents for use with immunoassays for antibodies
CN115397464A (zh) * 2020-04-24 2022-11-25 美国西门子医学诊断股份有限公司 用于使用抗人免疫球蛋白抗体的抗微生物血清学测定的组合物、试剂盒和方法
CN112014383B (zh) * 2020-06-30 2024-03-29 杜旭忠 一种用于均相化学发光的光敏剂及其制备方法和应用
CN114249754B (zh) * 2021-12-15 2022-11-25 大连理工大学 基于内过氧结构的分子氧输送剂及应用

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Also Published As

Publication number Publication date
WO2011143606A1 (en) 2011-11-17
US20130084652A1 (en) 2013-04-04
JP2013532275A (ja) 2013-08-15
EP2569442A1 (en) 2013-03-20
AU2011252833A1 (en) 2012-11-29
CN102892896A (zh) 2013-01-23
AU2011252833A2 (en) 2012-12-20
SG185409A1 (en) 2012-12-28
EP2569442A4 (en) 2013-10-09
KR20130091644A (ko) 2013-08-19

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