BR112012028848A2 - composições, organismos, sistemas e métodos para expressar um produto de gene em plantas - Google Patents

composições, organismos, sistemas e métodos para expressar um produto de gene em plantas

Info

Publication number
BR112012028848A2
BR112012028848A2 BR112012028848A BR112012028848A BR112012028848A2 BR 112012028848 A2 BR112012028848 A2 BR 112012028848A2 BR 112012028848 A BR112012028848 A BR 112012028848A BR 112012028848 A BR112012028848 A BR 112012028848A BR 112012028848 A2 BR112012028848 A2 BR 112012028848A2
Authority
BR
Brazil
Prior art keywords
ecs
exna
plant
nucleic acid
expression
Prior art date
Application number
BR112012028848A
Other languages
English (en)
Inventor
Eric T Mirkov
Original Assignee
Texas A & M Univ Sys
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Texas A & M Univ Sys filed Critical Texas A & M Univ Sys
Publication of BR112012028848A2 publication Critical patent/BR112012028848A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/34—Vector systems having a special element relevant for transcription being a transcription initiation element

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

composições ,organismos,sistemas e métodos para expressar um produto de gene em plantas"a presente revelação se refere,em algumas modalidades,a composições,organismos,sistemas e métodos para expressar um produto de gene em uma planta com o uso de sequência de expressão (ecs)operável em monocotiledonêas e/ou dicotiledõneas.por exemplo,(i) um ácido nucleico isolado pode compreender um ecs (por exemplo de vírus baciliforme de cana-de-açucar)e,opcionalmente,um ácido nucleico exógeno(exna)ligado de maneira operável ao ecs;(ii)um vetor de expressão pode compreender um ecs;umexna;eopcionalmente,uma sequência de terminação 3'em que o ecs tem atividade de promotor suficiente para expressar a exna em pelo menos monotiledônea e pelo menos uma dicotiledônea;(iii)um micro-organismo,célula de planta,ou planta pode compreender um ácido nucleico isolado;(iv)um método para expressar de forma constitutiva um exna em uma planta(por exemplo,uma monocotiledônea e/ou uma dicotiledônea)pode compreender,colocar um vetor de expressão em contato com o citosol de uma célula da planta,em que o vetor de expressão compreende o exna e um ecs e um ecs operável para acionar a expressão do exna'e/ou (v)um método para direcionar expressão constitutiva de um ácido nucleico em uma planta (por exemplo,uma monocotiledônea e/ou uma dicotiledônea)pode compreender transformar a planta com um ácido nucleico de expressão que compreende um ecs;exna e uma sequência de terminação 3.
BR112012028848A 2010-05-10 2011-05-10 composições, organismos, sistemas e métodos para expressar um produto de gene em plantas BR112012028848A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US39519710P 2010-05-10 2010-05-10
US40097610P 2010-08-05 2010-08-05
PCT/US2011/035908 WO2011143204A2 (en) 2010-05-10 2011-05-10 Compositions, organisms, systems, and methods for expressing a gene product in plants

Publications (1)

Publication Number Publication Date
BR112012028848A2 true BR112012028848A2 (pt) 2018-05-15

Family

ID=44352076

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012028848A BR112012028848A2 (pt) 2010-05-10 2011-05-10 composições, organismos, sistemas e métodos para expressar um produto de gene em plantas

Country Status (6)

Country Link
US (2) US8710207B2 (pt)
CN (2) CN107090453A (pt)
AR (1) AR081386A1 (pt)
AU (2) AU2011252008B2 (pt)
BR (1) BR112012028848A2 (pt)
WO (1) WO2011143204A2 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009262153B2 (en) * 2008-06-25 2014-02-27 BASF Agricultural Solutions Seed US LLC Toxin genes and methods for their use
CA2855125C (en) * 2011-12-30 2021-03-09 Dow Agrosciences Llc Method and construct for synthetic bidirectional scbv plant promoter
AR089830A1 (es) 2012-01-27 2014-09-17 Texas A & M Univ Sys Acido nucleico, vector de expresion, celula bacteriana, celula de planta de citrus y metodo de expresion de un acido nucleico exogeno en la planta de citrus
AU2013202941B2 (en) * 2012-02-29 2015-06-25 Dow Agrosciences Llc Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics
WO2015138499A1 (en) * 2014-03-10 2015-09-17 The Texas A&M University System Compositions, organisms, systems, and methods for expressing a gene product in plants
TWI530503B (zh) * 2014-07-21 2016-04-21 元智大學 修飾的啟動子序列及其應用
CN109402155A (zh) * 2018-11-12 2019-03-01 川北医学院 一种双控延迟裂解性质粒及其构建方法与应用
WO2021026689A1 (zh) * 2019-08-09 2021-02-18 北京大北农生物技术有限公司 用于检测大豆植物dbn8007的核酸序列及其检测方法
CN110452905B (zh) * 2019-08-22 2023-06-02 云南省烟草农业科学研究院 一种提高烟草dna沉淀效率的提取方法及其应用
CN114574492B (zh) * 2022-03-25 2023-09-19 广东省科学院南繁种业研究所 一种来自甘蔗杆状病毒的组成型启动子pscbv-chn1及其应用
CN114591981B (zh) * 2022-03-25 2023-05-26 江苏师范大学 Tppi基因在调控植物根系发育和促进植物茉莉酸积累方面的应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994123A (en) * 1996-08-09 1999-11-30 Regents Of University Of Minnesota Sugarcane bacilliform virus promoter
ATE526406T1 (de) * 1998-03-20 2011-10-15 Commw Scient Ind Res Org Kontrolle der genexpression
US6479636B1 (en) * 2000-04-11 2002-11-12 Honiron Corporation (A Louisiana Corporation) Sugarcane fractioning system
US20020042928A1 (en) * 2000-07-11 2002-04-11 Mccaslin Mark H. Methods for maximizing expression of transgenic traits in autopolyploid plants
AUPR160400A0 (en) * 2000-11-21 2000-12-14 Bureau Of Sugar Experiment Stations Plant virus promoter
US7001739B2 (en) 2001-08-24 2006-02-21 The Texas A&M University System Isolation of proteins involved in posttranscriptional gene silencing and methods of use
WO2003072750A2 (en) * 2002-02-27 2003-09-04 Prodigene, Inc. Expression of hiv-related proteins in plants
US7253276B2 (en) * 2003-01-03 2007-08-07 The Texas A&M University System Stem-regulated, plant defense promoter and uses thereof in tissue-specific expression in monocots

Also Published As

Publication number Publication date
AU2016203951B2 (en) 2018-12-06
US10202612B2 (en) 2019-02-12
US20140208462A1 (en) 2014-07-24
AU2016203951A1 (en) 2016-07-07
US20110283377A1 (en) 2011-11-17
WO2011143204A3 (en) 2012-01-05
AR081386A1 (es) 2012-08-29
AU2011252008B2 (en) 2016-03-17
WO2011143204A2 (en) 2011-11-17
US8710207B2 (en) 2014-04-29
CN103052712B (zh) 2016-10-12
CN107090453A (zh) 2017-08-25
CN103052712A (zh) 2013-04-17
AU2011252008A1 (en) 2012-11-29

Similar Documents

Publication Publication Date Title
BR112012028848A2 (pt) composições, organismos, sistemas e métodos para expressar um produto de gene em plantas
CL2012000197A1 (es) Ácidos nucleicos aislados correspondientes a promotores de expresion genica, casete de expresion y celula huesped que lo comprenden, y metodo para controlar la expresion genica en celulas oclusivas vegetales.
Illingworth et al. The diverse bacterial origins of the Arabidopsis polyamine biosynthetic pathway
BR112014018559A2 (pt) ácido nucleico; vetor de expressão; célula basteriana; célula de planta cítrica; planta cítrica; e método para expressar em uma planta cítrica um ácido nucleico exógeno que compreende uma sequência de ácido nucleico que codifica um peptídeo expresso
BRPI0910812A2 (pt) célula de fermentação de açúcar pentose
WO2012045082A3 (en) Engineered nucleic acids and methods of use thereof
BRPI1013483A2 (pt) Polipeptídeo isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, métodos para produzair o polipeptídeo e para degradar um xilano acetilado, planta transgênica, parte de planta ou célula de planta, composição, uso do polipeptídeo
BR112014019043A2 (pt) peptídeos de trânsito de cloroplasto derivados de brassica sintéticos
BR112012028805A2 (pt) composições de endorribonuclease e métodos de uso das mesmas.
BR112012011711A2 (pt) Variantes de beta-glucosidase 1 (bgl1) com propriedades otimizadas, seu método de produção, composição, ácido nucleico, vetor de expressão, célula hospedeira, bem como método de conversão de biomassa em açúcares
BRPI0814781A8 (pt) ''proteína, sequência de ácido nucleico, método para a produção de ácido itacônico, vetor, célula hospedeira, e, uso de um ácido cis-aconítico descarboxilase''
BR112012022108A2 (pt) engenharia microbial para a produção de ácidos graxos e derivados de ácido graxo
BR112012013875B8 (pt) método in vitro para reprogramar células somáticas humanas ou de outros mamíferos para células tronco pluripotentes induzidas (células ips ou ipscs) e composição
AR083355A1 (es) Polipeptidos variantes cbh i
BR112012000914B8 (pt) uso de polipeptídeo com atividade condrogênica, método in vitro de indução da diferenciação de células-tronco mesenquimais em condrócitos, e composição farmacêutica para aplicação intra-articular
AR094114A1 (es) Proteinas resistentes a herbicidas, genes que las codifican y sus usos
EA201400192A1 (ru) Клетка, ферментирующая пентозы
PH12020552291A1 (en) System and methods for engineering bacteria fit for eukaryotic mrna production, export, and translation in a eukaryotic host
WO2010061187A3 (en) Method for the transformation of plant cell mitochondria
MX2012001672A (es) Vector que comprende promotor de manosa y promotor de manosa.
BR112014012417A2 (pt) polipeptídeo e polinucleotídeo isolados, célula hospedeira recombinante, métodos para a produção de um polipeptídeo, de um mutante de uma célula de origem, e de uma proteína, e para a inibição da expressão de um polipeptídeo, planta transgênica, parte da planta ou célula de planta, molécula de rna, processos para degradar ou converter um material celulósico ou contendo xilano, para produzir um produto de fermentação, e de fermentação de um material celulósico ou contendo xilano, e, formulação de caldo integral ou composição de cultura de células
BR112012033396A2 (pt) Polipetídeo tendo atividade swollenin e seus usos.
HRP20170884T1 (hr) Oligonukleotidna sekvenca za upotrebu u inženjeringu bioloških puteva
MY171359A (en) Pentose fermenting microorganisms
BR112016020879A2 (pt) composições, organismos, sistemas e métodos para expressar um produto gênico em plantas

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL