BR112012027799A2 - aparelho de purificação de amostra biológica automático equipado com parte de aplicação de campo magnético, método de extração de substância-alvo da amostra biológica, e método de purificação e expressão de proteína - Google Patents

aparelho de purificação de amostra biológica automático equipado com parte de aplicação de campo magnético, método de extração de substância-alvo da amostra biológica, e método de purificação e expressão de proteína

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Publication number
BR112012027799A2
BR112012027799A2 BR112012027799A BR112012027799A BR112012027799A2 BR 112012027799 A2 BR112012027799 A2 BR 112012027799A2 BR 112012027799 A BR112012027799 A BR 112012027799A BR 112012027799 A BR112012027799 A BR 112012027799A BR 112012027799 A2 BR112012027799 A2 BR 112012027799A2
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BR
Brazil
Prior art keywords
biological sample
magnetic field
field application
target substance
automatic
Prior art date
Application number
BR112012027799A
Other languages
English (en)
Inventor
Han Oh Park
Jong Hoon Kim
Jong Kab Kim
Yang Won Lee
Original Assignee
Bioneer Corp
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Publication date
Application filed by Bioneer Corp filed Critical Bioneer Corp
Publication of BR112012027799A2 publication Critical patent/BR112012027799A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/16—Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
    • C12M1/18—Multiple fields or compartments
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

aparelho de purificação de amostra biológica automático equipado com parte de aplicação de campo magnético, método de extração de substância-alvo da amostra biológica, e método de purificação e expressão de proteína. a presente invenção refere-se a um dispositivo de purificação automática para separação de uma substância-alvo de uma pluralidade de amostras biológicas, e mais especificamente se refere a um dispositivo de purificação de amostra biológica automática possuindo uma unidade de aplicação de campo magnético para purificar a amostra biológica; a unidade deaplicação de campo magnético sendo integrada a uma parte de aquecimento de tal forma a permitir o movimento vertical. além disso, a presente invenção se refere a um método de extração de uma substãncia-alvo de uma mostra biológica pela utilização do dispositivo de purificação de amostra biológica automática possuindo a unidade de aplicação de campo magnético.
BR112012027799A 2010-04-30 2011-04-29 aparelho de purificação de amostra biológica automático equipado com parte de aplicação de campo magnético, método de extração de substância-alvo da amostra biológica, e método de purificação e expressão de proteína BR112012027799A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100040933 2010-04-30
PCT/KR2011/003245 WO2011136624A2 (ko) 2010-04-30 2011-04-29 자기장 인가부를 구비한 생물학적 시료 자동정제장치, 생물학적 시료로부터 타겟물질을 추출하는 방법, 그리고 단백질 발현 및 정제 방법

Publications (1)

Publication Number Publication Date
BR112012027799A2 true BR112012027799A2 (pt) 2015-11-24

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Family Applications (1)

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BR112012027799A BR112012027799A2 (pt) 2010-04-30 2011-04-29 aparelho de purificação de amostra biológica automático equipado com parte de aplicação de campo magnético, método de extração de substância-alvo da amostra biológica, e método de purificação e expressão de proteína

Country Status (8)

Country Link
US (1) US9187725B2 (pt)
EP (1) EP2565260B1 (pt)
KR (1) KR101443727B1 (pt)
BR (1) BR112012027799A2 (pt)
CL (1) CL2012003032A1 (pt)
ES (1) ES2863454T3 (pt)
RU (1) RU2545404C2 (pt)
WO (1) WO2011136624A2 (pt)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102427885B (zh) 2009-05-15 2016-10-19 简·探针公司 用于在执行磁性分离工序的仪器中实现磁体的自动移动的方法和设备
KR101400675B1 (ko) * 2010-11-18 2014-05-29 (주)바이오니아 에어로졸방지를 위한 자동정제장비 및 자동정제방법
KR20130023091A (ko) * 2011-08-26 2013-03-07 (주)바이오니아 단백질 합성 키트, 자동추출장비를 이용한 단백질 발현 및 추출 방법
KR101762295B1 (ko) * 2012-02-10 2017-08-04 (주)바이오니아 생체시료의 자동 분석 장치 및 방법
CN104884606B (zh) * 2012-11-30 2020-07-10 株式会社百奥尼 自动化制备无细胞蛋白的设备及其制备蛋白质的方法
CN103923830B (zh) * 2013-01-15 2016-06-29 常州金麦格生物技术有限公司 生物活性物质提取仪的微环境改良方法和系统
CN103923163B (zh) * 2013-01-15 2017-05-24 常州金麦格生物技术有限公司 用于生物活性物质提取仪的工作台系统和方法
JP6360559B2 (ja) * 2013-12-09 2018-07-18 バイオニア コーポレーション 磁性粒子分離装置、並びに、この装置を用いる核酸またはたんぱく質の分離及び精製方法
CN103756871B (zh) * 2013-12-30 2015-04-22 西安天隆科技有限公司 一种单样本核酸封闭式提取仪
CN103878061B (zh) * 2014-03-21 2016-05-25 东南大学 一种全自动的磁分离装置
KR102344395B1 (ko) * 2014-06-24 2021-12-29 (주)바이오니아 자성 입자를 이용한 핵산 분리 방법
US9358570B2 (en) * 2014-08-04 2016-06-07 Philip Hickman Viscous material warming device
KR102323205B1 (ko) * 2014-08-22 2021-11-08 삼성전자주식회사 표적물질 분리장치 및 표적물질 분리방법
US9663780B2 (en) 2014-10-15 2017-05-30 Alpaqua Engineering, LLC Solid-core ring-magnet
CN104450498B (zh) * 2014-12-16 2017-01-18 嘉兴凯实生物科技有限公司 一种核酸提取仪
US9943092B1 (en) * 2014-12-22 2018-04-17 Roy Lee Garrison Liquid processing system and method
CN104651225A (zh) * 2015-03-13 2015-05-27 润科生物工程（福建）有限公司 组合式手动微生物接种器
CN106554901B (zh) * 2015-09-30 2019-06-14 精专生医股份有限公司 自动化萃取核酸的机台及配合其使用的针筒
EP3213819B1 (en) * 2016-03-02 2021-09-29 F. Hoffmann-La Roche AG Device and method for separation
ES2862393T3 (es) 2016-05-03 2021-10-07 Biomerieux Sa Procedimiento y sistema de extracción magnética de componentes en una muestra líquida
CN109153957B (zh) * 2016-05-26 2021-07-06 株式会社Ｉｆｇ 培养液中的细胞的非接触电刺激装置和非接触电刺激方法
KR102256775B1 (ko) * 2016-09-29 2021-05-27 (주)바이오니아 생물학적 시료 처리장치
WO2018070984A1 (en) * 2016-10-10 2018-04-19 Hewlett-Packard Development Company, L.P. Fluid operation cell with on-chip electrical fluid operation components
RU2643385C1 (ru) * 2016-11-11 2018-02-01 Общество с ограниченной ответственностью "ДРД" Устройство для разделения магнитных частиц из реакционных жидкостей
CN106867883B (zh) * 2017-03-03 2019-12-27 安徽为臻生物工程技术有限公司 一种自动核酸提取仪
CN106676002A (zh) * 2017-03-08 2017-05-17 深圳市爱康生物科技有限公司 一种应用于自动核酸提取仪的自动磁力架
CN107287194A (zh) * 2017-07-31 2017-10-24 南京中科拜尔医学技术有限公司 提取装置
RU2714734C1 (ru) * 2018-03-19 2020-02-19 Общество с ограниченной активностью "Синтол" (ООО "Синтол") Устройство для автоматической очистки биологических образцов
CN108277148B (zh) * 2018-04-25 2024-05-28 江苏硕世生物科技股份有限公司 核酸提取分液装置
KR102256776B1 (ko) * 2018-07-26 2021-05-27 (주)바이오니아 자석봉 블록의 교체가 가능한 표적물질 추출장치
US11242519B2 (en) 2018-08-23 2022-02-08 Alpaqua Engineering, LLC Discontinuous wall hollow core magnet
CN109459287B (zh) * 2018-12-12 2020-06-05 浙江大学 一种基于微流控芯片的连续磁分离装置及方法
US20220091147A1 (en) * 2019-01-28 2022-03-24 Formulatrix, Inc. Liquid handling instrument and pipetting head for and method of aspirating and/or dispensing liquids
CN110376124B (zh) * 2019-07-10 2022-03-25 金华职业技术学院 一种生物样品拉伸测试方法
CN111686617B (zh) * 2020-05-08 2022-06-17 南京涵曦月自动化科技有限公司 一种环保家具漆用粉体添加混合装置
CN111751195A (zh) * 2020-06-29 2020-10-09 中国水产科学研究院 一种多通道自动净化装置
KR20220075978A (ko) 2020-11-30 2022-06-08 (주)바이오니아 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법
KR102512066B1 (ko) * 2021-04-29 2023-03-21 주식회사 제놀루션 핵산추출장치
CN113695074B (zh) * 2021-07-29 2022-09-09 广西建工集团第四建筑工程有限责任公司 一种土木工程绿色建筑废料处理装置
CN114574349B (zh) * 2022-04-26 2022-10-14 广州国家实验室 一种适配卡盒使用的设备
CN116731856B (zh) * 2023-04-24 2023-12-01 扬州市职业大学(扬州开放大学) 一种高通量自动分选装置及其分选方法
CN116523920B (zh) * 2023-07-05 2023-09-08 山东万里红信息技术有限公司 一种基于视觉监测的磁介质销毁处理方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988618A (en) 1987-11-16 1991-01-29 Gene-Trak Systems Magnetic separation device and methods for use in heterogeneous assays
JP2701891B2 (ja) 1987-11-16 1998-01-21 ジーン−トラック・システムス 磁気的分離デバイスおよび不均質検定における使用法
EP0478753B1 (en) 1990-04-06 1997-07-02 The Perkin-Elmer Corporation Automated molecular biology laboratory
EP0479448A3 (en) 1990-10-02 1992-12-23 Beckman Instruments, Inc. Magnetic separation device
US5897783A (en) 1992-09-24 1999-04-27 Amersham International Plc Magnetic separation method
DE69429159T2 (de) * 1993-02-01 2002-08-14 Thermo Labsystems Oy, Helsinki Verfahren für einen spezifischen Bindungstest mit magnetischen Teilchen
FI932866A0 (fi) 1993-06-21 1993-06-21 Labsystems Oy Separeringsfoerfarande
JP3115501B2 (ja) 1994-06-15 2000-12-11 プレシジョン・システム・サイエンス株式会社 分注機を利用した磁性体の脱着制御方法及びこの方法によって処理される各種装置
US5789251A (en) * 1994-06-16 1998-08-04 Astle; Thomas W. Multi-well bioassay tray with evaporation protection and method of use
DE4423878A1 (de) 1994-07-07 1996-01-11 Boehringer Mannheim Gmbh Vorrichtung und Verfahren zum Abscheiden von magnetischen Mikropartikeln
US6672458B2 (en) * 2000-05-19 2004-01-06 Becton, Dickinson And Company System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample
JP2002001092A (ja) * 2000-06-22 2002-01-08 Shimadzu Corp 排液装置
AU2003287603A1 (en) 2002-11-08 2004-06-03 Pharmacia Corportion High throughput automatic nucleic acid isolation and quantitation methods
KR100483684B1 (ko) 2003-01-29 2005-04-18 (주)바이오넥스 핵산 또는 다양한 생물학적 물질을 분리 및 정제하기 위한키트와, 이러한 키트를 이용하여 생물학적 물질의 분리또는 정제 작업을 자동화하기 위한 시스템
JP4021335B2 (ja) * 2003-01-31 2007-12-12 ユニバーサル・バイオ・リサーチ株式会社 監視機能付分注装置および分注装置の監視方法
IL155897A0 (en) * 2003-05-13 2003-12-23 Amon Valinsky An indicator for multiwell plate and method for using the same
JP5079506B2 (ja) * 2004-07-30 2012-11-21 プルリセレクト ゲーエムベーハー 動物についての、バイオテクノロジー（生物学的研究を含む）および医学的診断での適用のための、細胞、生体粒子および／または分子を液体から単離するための装置ならびに方法
JP4656646B2 (ja) * 2005-10-04 2011-03-23 キヤノン株式会社 表示方法と表示装置、該表示装置を備えた全自動検査装置
EP1785189A1 (en) * 2005-11-11 2007-05-16 Roche Diagnostics GmbH Drop Catcher
US8133671B2 (en) * 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
KR101025135B1 (ko) * 2008-04-09 2011-03-31 (주)바이오니아 자동정제장치, 멀티 웰 플레이트 키트 및 생물학적 시료로부터 핵산을 추출하는 방법
RU2380418C1 (ru) * 2008-10-01 2010-01-27 Учреждение Российской академии наук Институт молекулярной биологии им. В.А. Энгельгардта РАН Сменный микрофлюидный модуль для автоматизированного выделения и очистки нуклеиновых кислот из биологических образцов и способ выделения и очистки нуклеиновых кислот с его использованием
RU84381U1 (ru) * 2009-02-17 2009-07-10 Учреждение Российской Академии Наук Институт Молекулярной Биологии Им. В.А. Энгельгардта Ран (Имб Ран) Устройство для автоматизированного выделения нуклеиновых кислот
KR101420094B1 (ko) * 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
KR101400675B1 (ko) * 2010-11-18 2014-05-29 (주)바이오니아 에어로졸방지를 위한 자동정제장비 및 자동정제방법

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Publication number Publication date
EP2565260A4 (en) 2014-03-12
KR101443727B1 (ko) 2014-09-26
US20130043191A1 (en) 2013-02-21
EP2565260A2 (en) 2013-03-06
WO2011136624A2 (ko) 2011-11-03
ES2863454T3 (es) 2021-10-11
RU2545404C2 (ru) 2015-03-27
WO2011136624A3 (ko) 2012-03-01
RU2012151009A (ru) 2014-06-10
KR20110121588A (ko) 2011-11-07
EP2565260B1 (en) 2020-12-23
US9187725B2 (en) 2015-11-17
CL2012003032A1 (es) 2013-06-07

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