BR112012026219B1 - método para o diagnóstico de glaucoma, elementos carreadores de antígeno e kit - Google Patents
método para o diagnóstico de glaucoma, elementos carreadores de antígeno e kit Download PDFInfo
- Publication number
- BR112012026219B1 BR112012026219B1 BR112012026219A BR112012026219A BR112012026219B1 BR 112012026219 B1 BR112012026219 B1 BR 112012026219B1 BR 112012026219 A BR112012026219 A BR 112012026219A BR 112012026219 A BR112012026219 A BR 112012026219A BR 112012026219 B1 BR112012026219 B1 BR 112012026219B1
- Authority
- BR
- Brazil
- Prior art keywords
- glaucoma
- antigens
- ocular
- antigen
- protein
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title claims abstract description 338
- 102000036639 antigens Human genes 0.000 title claims abstract description 335
- 108091007433 antigens Proteins 0.000 title claims abstract description 335
- 208000010412 Glaucoma Diseases 0.000 title claims abstract description 250
- 238000000034 method Methods 0.000 title claims abstract description 100
- 230000009257 reactivity Effects 0.000 claims abstract description 103
- 210000001124 body fluid Anatomy 0.000 claims abstract description 78
- 239000010839 body fluid Substances 0.000 claims abstract description 78
- 230000001363 autoimmune Effects 0.000 claims abstract description 70
- 238000002405 diagnostic procedure Methods 0.000 claims abstract description 52
- 238000012360 testing method Methods 0.000 claims abstract description 48
- 238000002493 microarray Methods 0.000 claims description 30
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 28
- -1 beta- S-crystalline Proteins 0.000 claims description 27
- 108010071690 Prealbumin Proteins 0.000 claims description 26
- 238000003745 diagnosis Methods 0.000 claims description 25
- 102000004121 Annexin A5 Human genes 0.000 claims description 21
- 108090000672 Annexin A5 Proteins 0.000 claims description 21
- 102000015395 alpha 1-Antitrypsin Human genes 0.000 claims description 21
- 108010050122 alpha 1-Antitrypsin Proteins 0.000 claims description 21
- 229940024142 alpha 1-antitrypsin Drugs 0.000 claims description 21
- 102000004219 Brain-derived neurotrophic factor Human genes 0.000 claims description 19
- 108090000715 Brain-derived neurotrophic factor Proteins 0.000 claims description 19
- 108090000099 Neurotrophin-4 Proteins 0.000 claims description 19
- 102000044159 Ubiquitin Human genes 0.000 claims description 18
- 108090000848 Ubiquitin Proteins 0.000 claims description 18
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims description 18
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims description 18
- 229940077737 brain-derived neurotrophic factor Drugs 0.000 claims description 18
- 102000009190 Transthyretin Human genes 0.000 claims description 17
- 102100039165 Heat shock protein beta-1 Human genes 0.000 claims description 16
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 claims description 16
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 claims description 16
- 101710163595 Chaperone protein DnaK Proteins 0.000 claims description 15
- 108010045100 HSP27 Heat-Shock Proteins Proteins 0.000 claims description 15
- 101710178376 Heat shock 70 kDa protein Proteins 0.000 claims description 15
- 101710152018 Heat shock cognate 70 kDa protein Proteins 0.000 claims description 15
- 101710175516 14 kDa zinc-binding protein Proteins 0.000 claims description 14
- 102000007469 Actins Human genes 0.000 claims description 14
- 102000004877 Insulin Human genes 0.000 claims description 14
- 108090001061 Insulin Proteins 0.000 claims description 14
- 229940123924 Protein kinase C inhibitor Drugs 0.000 claims description 14
- 102000004338 Transferrin Human genes 0.000 claims description 14
- 108090000901 Transferrin Proteins 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 14
- 229940125396 insulin Drugs 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 14
- 239000003881 protein kinase C inhibitor Substances 0.000 claims description 14
- 239000012581 transferrin Substances 0.000 claims description 14
- 102100038222 60 kDa heat shock protein, mitochondrial Human genes 0.000 claims description 13
- 108010085238 Actins Proteins 0.000 claims description 13
- 102000000412 Annexin Human genes 0.000 claims description 13
- 108050008874 Annexin Proteins 0.000 claims description 13
- 102000004082 Calreticulin Human genes 0.000 claims description 13
- 108090000549 Calreticulin Proteins 0.000 claims description 13
- 108010059013 Chaperonin 10 Proteins 0.000 claims description 13
- 108010058432 Chaperonin 60 Proteins 0.000 claims description 13
- 108050001186 Chaperonin Cpn60 Proteins 0.000 claims description 13
- 102000052603 Chaperonins Human genes 0.000 claims description 13
- 102000003923 Protein Kinase C Human genes 0.000 claims description 13
- 108090000315 Protein Kinase C Proteins 0.000 claims description 13
- 102000019197 Superoxide Dismutase Human genes 0.000 claims description 13
- 108010012715 Superoxide dismutase Proteins 0.000 claims description 13
- 102000009027 Albumins Human genes 0.000 claims description 12
- 108010088751 Albumins Proteins 0.000 claims description 12
- 102000004230 Neurotrophin 3 Human genes 0.000 claims description 12
- 108090000742 Neurotrophin 3 Proteins 0.000 claims description 12
- 102000009843 Thyroglobulin Human genes 0.000 claims description 12
- 108010034949 Thyroglobulin Proteins 0.000 claims description 12
- 102000004963 gamma-Synuclein Human genes 0.000 claims description 12
- 108090001121 gamma-Synuclein Proteins 0.000 claims description 12
- 229940032018 neurotrophin 3 Drugs 0.000 claims description 12
- 229960002175 thyroglobulin Drugs 0.000 claims description 12
- 108010067306 Fibronectins Proteins 0.000 claims description 11
- 102000016359 Fibronectins Human genes 0.000 claims description 11
- 108010070675 Glutathione transferase Proteins 0.000 claims description 11
- 102000006386 Myelin Proteins Human genes 0.000 claims description 11
- 108010062374 Myoglobin Proteins 0.000 claims description 11
- 102000036675 Myoglobin Human genes 0.000 claims description 11
- 102100033857 Neurotrophin-4 Human genes 0.000 claims description 11
- 101710119265 DNA topoisomerase 1 Proteins 0.000 claims description 10
- 102100024607 DNA topoisomerase 1 Human genes 0.000 claims description 10
- 102000016943 Muramidase Human genes 0.000 claims description 10
- 108010014251 Muramidase Proteins 0.000 claims description 10
- 108010083674 Myelin Proteins Proteins 0.000 claims description 10
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 10
- 238000004422 calculation algorithm Methods 0.000 claims description 10
- ZGSPNIOCEDOHGS-UHFFFAOYSA-L disodium [3-[2,3-di(octadeca-9,12-dienoyloxy)propoxy-oxidophosphoryl]oxy-2-hydroxypropyl] 2,3-di(octadeca-9,12-dienoyloxy)propyl phosphate Chemical compound [Na+].[Na+].CCCCCC=CCC=CCCCCCCCC(=O)OCC(OC(=O)CCCCCCCC=CCC=CCCCCC)COP([O-])(=O)OCC(O)COP([O-])(=O)OCC(OC(=O)CCCCCCCC=CCC=CCCCCC)COC(=O)CCCCCCCC=CCC=CCCCCC ZGSPNIOCEDOHGS-UHFFFAOYSA-L 0.000 claims description 10
- 229960000274 lysozyme Drugs 0.000 claims description 10
- 235000010335 lysozyme Nutrition 0.000 claims description 10
- 239000004325 lysozyme Substances 0.000 claims description 10
- 210000005012 myelin Anatomy 0.000 claims description 10
- 150000002978 peroxides Chemical class 0.000 claims description 10
- 102000014914 Carrier Proteins Human genes 0.000 claims description 9
- 102000005890 Spectrin Human genes 0.000 claims description 9
- 108010019965 Spectrin Proteins 0.000 claims description 9
- 102100029290 Transthyretin Human genes 0.000 claims description 9
- 108010065472 Vimentin Proteins 0.000 claims description 9
- 108090000185 alpha-Synuclein Proteins 0.000 claims description 9
- 108091008324 binding proteins Proteins 0.000 claims description 9
- BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 claims description 9
- 210000005048 vimentin Anatomy 0.000 claims description 9
- 102000003683 Neurotrophin-4 Human genes 0.000 claims description 8
- 108010005298 Oligodendrocyte-Myelin Glycoprotein Proteins 0.000 claims description 8
- 102100026746 Oligodendrocyte-myelin glycoprotein Human genes 0.000 claims description 8
- 239000003534 dna topoisomerase inhibitor Substances 0.000 claims description 8
- 230000001537 neural effect Effects 0.000 claims description 8
- 229940097998 neurotrophin 4 Drugs 0.000 claims description 8
- 229940044693 topoisomerase inhibitor Drugs 0.000 claims description 8
- 238000003556 assay Methods 0.000 claims description 7
- 101710193519 Glial fibrillary acidic protein Proteins 0.000 claims description 6
- 230000003511 endothelial effect Effects 0.000 claims description 6
- 210000005046 glial fibrillary acidic protein Anatomy 0.000 claims description 6
- 239000003102 growth factor Substances 0.000 claims description 6
- 230000006444 vascular growth Effects 0.000 claims description 6
- 230000000890 antigenic effect Effects 0.000 claims description 4
- 108010014499 beta-2 Adrenergic Receptors Proteins 0.000 claims description 3
- 102000016966 beta-2 Adrenergic Receptors Human genes 0.000 claims description 3
- 239000002250 absorbent Substances 0.000 claims description 2
- 230000002745 absorbent Effects 0.000 claims description 2
- 238000011156 evaluation Methods 0.000 claims description 2
- 102100039289 Glial fibrillary acidic protein Human genes 0.000 claims 2
- 102100029100 Hematopoietic prostaglandin D synthase Human genes 0.000 claims 2
- 102000013127 Vimentin Human genes 0.000 claims 2
- 102000005962 receptors Human genes 0.000 claims 2
- 108020003175 receptors Proteins 0.000 claims 2
- 102100026882 Alpha-synuclein Human genes 0.000 claims 1
- 102000004145 Annexin A1 Human genes 0.000 claims 1
- 108090000663 Annexin A1 Proteins 0.000 claims 1
- 101100072629 Drosophila melanogaster ine gene Proteins 0.000 claims 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 abstract description 186
- 102000004169 proteins and genes Human genes 0.000 abstract description 182
- 238000004458 analytical method Methods 0.000 abstract description 60
- 230000014509 gene expression Effects 0.000 abstract description 40
- 238000011282 treatment Methods 0.000 abstract description 31
- 238000011534 incubation Methods 0.000 abstract description 16
- 230000003833 cell viability Effects 0.000 abstract description 12
- 238000002360 preparation method Methods 0.000 abstract description 8
- 238000012606 in vitro cell culture Methods 0.000 abstract description 5
- 239000008194 pharmaceutical composition Substances 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 173
- 210000002966 serum Anatomy 0.000 description 141
- 206010030348 Open-Angle Glaucoma Diseases 0.000 description 78
- 201000006366 primary open angle glaucoma Diseases 0.000 description 78
- 239000000523 sample Substances 0.000 description 58
- 210000001742 aqueous humor Anatomy 0.000 description 49
- 239000000090 biomarker Substances 0.000 description 49
- 238000001514 detection method Methods 0.000 description 24
- 230000035899 viability Effects 0.000 description 24
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 23
- 210000003994 retinal ganglion cell Anatomy 0.000 description 20
- 210000001138 tear Anatomy 0.000 description 20
- 230000035945 sensitivity Effects 0.000 description 19
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 18
- 230000004410 intraocular pressure Effects 0.000 description 18
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 15
- 238000013528 artificial neural network Methods 0.000 description 15
- 238000004113 cell culture Methods 0.000 description 14
- 206010067013 Normal tension glaucoma Diseases 0.000 description 13
- 206010030043 Ocular hypertension Diseases 0.000 description 13
- 230000006907 apoptotic process Effects 0.000 description 13
- 201000002978 low tension glaucoma Diseases 0.000 description 13
- 239000002953 phosphate buffered saline Substances 0.000 description 13
- 108010000123 Myelin-Oligodendrocyte Glycoprotein Proteins 0.000 description 12
- 102000002233 Myelin-Oligodendrocyte Glycoprotein Human genes 0.000 description 12
- 230000000694 effects Effects 0.000 description 12
- 108010033040 Histones Proteins 0.000 description 11
- 238000004949 mass spectrometry Methods 0.000 description 11
- 239000002253 acid Substances 0.000 description 10
- 238000000540 analysis of variance Methods 0.000 description 10
- 230000009089 cytolysis Effects 0.000 description 10
- 238000010195 expression analysis Methods 0.000 description 10
- 239000002609 medium Substances 0.000 description 10
- 230000002207 retinal effect Effects 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 102000005720 Glutathione transferase Human genes 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 201000010099 disease Diseases 0.000 description 9
- 239000000499 gel Substances 0.000 description 9
- 238000000338 in vitro Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 102000003802 alpha-Synuclein Human genes 0.000 description 8
- 230000008901 benefit Effects 0.000 description 8
- 239000013592 cell lysate Substances 0.000 description 8
- 230000000875 corresponding effect Effects 0.000 description 8
- 230000002518 glial effect Effects 0.000 description 8
- 230000004770 neurodegeneration Effects 0.000 description 8
- 208000015122 neurodegenerative disease Diseases 0.000 description 8
- 239000000020 Nitrocellulose Substances 0.000 description 7
- 102100035071 Vimentin Human genes 0.000 description 7
- 230000003247 decreasing effect Effects 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 238000003018 immunoassay Methods 0.000 description 7
- 239000011159 matrix material Substances 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 229920001220 nitrocellulos Polymers 0.000 description 7
- 238000012549 training Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 208000023275 Autoimmune disease Diseases 0.000 description 6
- 102000006947 Histones Human genes 0.000 description 6
- 241000282414 Homo sapiens Species 0.000 description 6
- 239000003463 adsorbent Substances 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 230000030833 cell death Effects 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 102000053171 Glial Fibrillary Acidic Human genes 0.000 description 5
- 238000004364 calculation method Methods 0.000 description 5
- 238000002508 contact lithography Methods 0.000 description 5
- HKSZLNNOFSGOKW-UHFFFAOYSA-N ent-staurosporine Natural products C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1C1CC(NC)C(OC)C4(C)O1 HKSZLNNOFSGOKW-UHFFFAOYSA-N 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000002372 labelling Methods 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- 239000002243 precursor Substances 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 238000007619 statistical method Methods 0.000 description 5
- HKSZLNNOFSGOKW-FYTWVXJKSA-N staurosporine Chemical compound C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1[C@H]1C[C@@H](NC)[C@@H](OC)[C@]4(C)O1 HKSZLNNOFSGOKW-FYTWVXJKSA-N 0.000 description 5
- CGPUWJWCVCFERF-UHFFFAOYSA-N staurosporine Natural products C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1C1CC(NC)C(OC)C4(OC)O1 CGPUWJWCVCFERF-UHFFFAOYSA-N 0.000 description 5
- 238000000756 surface-enhanced laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 5
- 230000001225 therapeutic effect Effects 0.000 description 5
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 4
- 102000004142 Trypsin Human genes 0.000 description 4
- 108090000631 Trypsin Proteins 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 210000004556 brain Anatomy 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 4
- 238000003795 desorption Methods 0.000 description 4
- 239000012091 fetal bovine serum Substances 0.000 description 4
- 238000012125 lateral flow test Methods 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 210000001328 optic nerve Anatomy 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 238000007639 printing Methods 0.000 description 4
- 239000012588 trypsin Substances 0.000 description 4
- 238000012800 visualization Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 201000004569 Blindness Diseases 0.000 description 3
- 208000002177 Cataract Diseases 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 3
- 102100034523 Histone H4 Human genes 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 239000012298 atmosphere Substances 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 210000004292 cytoskeleton Anatomy 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000002224 dissection Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 208000030533 eye disease Diseases 0.000 description 3
- 125000000524 functional group Chemical group 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 238000010324 immunological assay Methods 0.000 description 3
- 239000012139 lysis buffer Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000000750 progressive effect Effects 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 102000003915 DNA Topoisomerases Human genes 0.000 description 2
- 108090000323 DNA Topoisomerases Proteins 0.000 description 2
- 206010061818 Disease progression Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108050001049 Extracellular proteins Proteins 0.000 description 2
- 101710100504 Heat shock protein beta-1 Proteins 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 208000018737 Parkinson disease Diseases 0.000 description 2
- 108010033276 Peptide Fragments Proteins 0.000 description 2
- 102000007079 Peptide Fragments Human genes 0.000 description 2
- 101710189920 Peptidyl-alpha-hydroxyglycine alpha-amidating lyase Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 101710120037 Toxin CcdB Proteins 0.000 description 2
- 241000364021 Tulsa Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 2
- 230000008827 biological function Effects 0.000 description 2
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 2
- 239000002981 blocking agent Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 238000003570 cell viability assay Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000000205 computational method Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 230000005750 disease progression Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000001516 effect on protein Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 230000002706 hydrostatic effect Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 238000010249 in-situ analysis Methods 0.000 description 2
- 230000002427 irreversible effect Effects 0.000 description 2
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000017074 necrotic cell death Effects 0.000 description 2
- 210000004498 neuroglial cell Anatomy 0.000 description 2
- 210000002569 neuron Anatomy 0.000 description 2
- 238000010606 normalization Methods 0.000 description 2
- 238000012014 optical coherence tomography Methods 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 230000008506 pathogenesis Effects 0.000 description 2
- 238000003498 protein array Methods 0.000 description 2
- 238000001742 protein purification Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000003938 response to stress Effects 0.000 description 2
- 210000001525 retina Anatomy 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000000672 surface-enhanced laser desorption--ionisation Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- 230000028973 vesicle-mediated transport Effects 0.000 description 2
- 230000004393 visual impairment Effects 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 description 1
- KISWVXRQTGLFGD-UHFFFAOYSA-N 2-[[2-[[6-amino-2-[[2-[[2-[[5-amino-2-[[2-[[1-[2-[[6-amino-2-[(2,5-diamino-5-oxopentanoyl)amino]hexanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]pyrrolidine-2-carbonyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-(diaminomethylideneamino)p Chemical compound C1CCN(C(=O)C(CCCN=C(N)N)NC(=O)C(CCCCN)NC(=O)C(N)CCC(N)=O)C1C(=O)NC(CO)C(=O)NC(CCC(N)=O)C(=O)NC(CCCN=C(N)N)C(=O)NC(CO)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CC(C)C)C(O)=O)CC1=CC=C(O)C=C1 KISWVXRQTGLFGD-UHFFFAOYSA-N 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- WBYWAXJHAXSJNI-SREVYHEPSA-N Cinnamic acid Chemical compound OC(=O)\C=C/C1=CC=CC=C1 WBYWAXJHAXSJNI-SREVYHEPSA-N 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 102000010831 Cytoskeletal Proteins Human genes 0.000 description 1
- 108010037414 Cytoskeletal Proteins Proteins 0.000 description 1
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
- 240000008168 Ficus benjamina Species 0.000 description 1
- 208000003098 Ganglion Cysts Diseases 0.000 description 1
- 108700005000 Glial Fibrillary Acidic Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000617823 Homo sapiens Solute carrier organic anion transporter family member 6A1 Proteins 0.000 description 1
- 101000662009 Homo sapiens UDP-N-acetylglucosamine pyrophosphorylase Proteins 0.000 description 1
- 101000621427 Homo sapiens Wiskott-Aldrich syndrome protein Proteins 0.000 description 1
- 101000839464 Leishmania braziliensis Heat shock 70 kDa protein Proteins 0.000 description 1
- VAYOSLLFUXYJDT-RDTXWAMCSA-N Lysergic acid diethylamide Chemical compound C1=CC(C=2[C@H](N(C)C[C@@H](C=2)C(=O)N(CC)CC)C2)=C3C2=CNC3=C1 VAYOSLLFUXYJDT-RDTXWAMCSA-N 0.000 description 1
- 102000002151 Microfilament Proteins Human genes 0.000 description 1
- 208000028389 Nerve injury Diseases 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 208000025966 Neurological disease Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 102100028251 Phosphoglycerate kinase 1 Human genes 0.000 description 1
- 101710139464 Phosphoglycerate kinase 1 Proteins 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
- 102100021991 Solute carrier organic anion transporter family member 6A1 Human genes 0.000 description 1
- 208000005400 Synovial Cyst Diseases 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 101710183280 Topoisomerase Proteins 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 102100037921 UDP-N-acetylglucosamine pyrophosphorylase Human genes 0.000 description 1
- 206010047555 Visual field defect Diseases 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 108091000387 actin binding proteins Proteins 0.000 description 1
- 108091009126 actinin binding proteins Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002730 additional effect Effects 0.000 description 1
- 230000001800 adrenalinergic effect Effects 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000006470 autoimmune attack Effects 0.000 description 1
- 230000005784 autoimmunity Effects 0.000 description 1
- 210000003050 axon Anatomy 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 229930016911 cinnamic acid Natural products 0.000 description 1
- 235000013985 cinnamic acid Nutrition 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003436 cytoskeletal effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000013501 data transformation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011496 digital image analysis Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 230000004406 elevated intraocular pressure Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000003114 enzyme-linked immunosorbent spot assay Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 239000010200 folin Substances 0.000 description 1
- 230000000574 ganglionic effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000000589 high-performance liquid chromatography-mass spectrometry Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229940043355 kinase inhibitor Drugs 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000001906 matrix-assisted laser desorption--ionisation mass spectrometry Methods 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 230000036651 mood Effects 0.000 description 1
- 238000000491 multivariate analysis Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000008764 nerve damage Effects 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000003705 neurological process Effects 0.000 description 1
- 210000001623 nucleosome Anatomy 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 210000003733 optic disk Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000009931 pascalization Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 210000001428 peripheral nervous system Anatomy 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 108091008695 photoreceptors Proteins 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000010149 post-hoc-test Methods 0.000 description 1
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 239000003909 protein kinase inhibitor Substances 0.000 description 1
- 239000012474 protein marker Substances 0.000 description 1
- 238000013138 pruning Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000036647 reaction Effects 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- 239000012146 running buffer Substances 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- AIDBEARHLBRLMO-UHFFFAOYSA-M sodium;dodecyl sulfate;2-morpholin-4-ylethanesulfonic acid Chemical compound [Na+].OS(=O)(=O)CCN1CCOCC1.CCCCCCCCCCCCOS([O-])(=O)=O AIDBEARHLBRLMO-UHFFFAOYSA-M 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000012453 sprague-dawley rat model Methods 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229940032362 superoxide dismutase Drugs 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 238000001920 surface-enhanced laser desorption--ionisation mass spectrometry Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000000946 synaptic effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000004154 testing of material Methods 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000001269 time-of-flight mass spectrometry Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000003325 tomography Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 230000004382 visual function Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 210000004127 vitreous body Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
- A61P27/06—Antiglaucoma agents or miotics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/16—Ophthalmology
- G01N2800/168—Glaucoma
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Ophthalmology & Optometry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Rehabilitation Therapy (AREA)
- Pharmacology & Pharmacy (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Rheumatology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34236310P | 2010-04-13 | 2010-04-13 | |
| PCT/CH2011/000077 WO2011127616A1 (en) | 2010-04-13 | 2011-04-13 | Diagnostic methods for glaucoma |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112012026219A2 BR112012026219A2 (pt) | 2016-07-12 |
| BR112012026219B1 true BR112012026219B1 (pt) | 2019-12-10 |
Family
ID=43984128
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112012026219A BR112012026219B1 (pt) | 2010-04-13 | 2011-04-13 | método para o diagnóstico de glaucoma, elementos carreadores de antígeno e kit |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US10175251B2 (https=) |
| EP (1) | EP2558862B1 (https=) |
| JP (2) | JP2013529292A (https=) |
| KR (1) | KR101903193B1 (https=) |
| CN (1) | CN103003693B (https=) |
| AU (1) | AU2011241402B2 (https=) |
| BR (1) | BR112012026219B1 (https=) |
| CA (1) | CA2795270C (https=) |
| DK (1) | DK2558862T3 (https=) |
| ES (1) | ES2712060T3 (https=) |
| HR (1) | HRP20190278T1 (https=) |
| HU (1) | HUE042703T2 (https=) |
| PL (1) | PL2558862T3 (https=) |
| RU (1) | RU2657515C2 (https=) |
| TR (1) | TR201902542T4 (https=) |
| WO (1) | WO2011127616A1 (https=) |
| ZA (1) | ZA201207935B (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012118863A1 (en) * | 2011-02-28 | 2012-09-07 | The Schepens Eye Research Institute, Inc. | Therapies that target autoimmunity for treating glaucoma and optic neuropathy |
| US10842849B2 (en) | 2011-02-28 | 2020-11-24 | The Schepens Eye Research Institute, Inc. | Methods for promoting neuronal outgrowth by insulin-like growth factor binding protein-like 1 (IGFBPL-1) in glaucoma or leber's optic neuropathy |
| WO2013126465A1 (en) * | 2012-02-21 | 2013-08-29 | MEDEIROS, Felipe, A. | Systems and methods for determining retinal ganglion cell populations and associated treatments |
| EP2770326B1 (en) * | 2013-02-26 | 2019-09-11 | Instituto Oftalmologico Fernandez - Vega, S.L. | Method for the diagnosis of glaucoma based on the determination of serum protein levels |
| WO2016060917A2 (en) * | 2014-10-15 | 2016-04-21 | Rapid Pathogen Screening, Inc. | Formulations for histatin therapeutics |
| JP6499918B2 (ja) | 2015-05-20 | 2019-04-10 | 株式会社トプコン | 眼科検査支援システム及び眼科検査支援サーバ |
| RU2617066C1 (ru) * | 2016-04-01 | 2017-04-19 | Федеральное государственное автономное учреждение "Межотраслевой научно-технический комплекс "Микрохирургия глаза" имени академика С.Н. Федорова" Министерства здравоохранения Российской Федерации | Способ определения показаний к интравитреальному введению цилиарного нейротрофического фактора при лечении заболеваний зрительно-нервного аппарата глаза |
| CN110213987A (zh) | 2016-12-05 | 2019-09-06 | 杰克逊实验室 | 视网膜和视神经中的脂肪滴作为人类的神经变性和青光眼的诊断标志物 |
| JP7202609B2 (ja) * | 2017-12-13 | 2023-01-12 | 国立大学法人東北大学 | 視神経障害の診断用バイオマーカー |
| CN108229545B (zh) * | 2017-12-22 | 2021-09-14 | 北京市商汤科技开发有限公司 | 青光眼诊断的方法、装置及电子设备 |
| US20220143139A1 (en) * | 2019-03-04 | 2022-05-12 | The Schepens Eye Research Institute, Inc. | Modulating Neuroinflammation |
| CN111856037B (zh) * | 2020-07-31 | 2021-06-22 | 四川大学华西第二医院 | Unc45a与hsp10的比值在pop早期预警、诊断、预后评估中的应用和产品 |
| KR102596340B1 (ko) | 2020-08-25 | 2023-11-02 | 사회복지법인 삼성생명공익재단 | 안질환 시야 손상 예후 예측 시스템 및 방법과 이를 위한 컴퓨터 프로그램 |
| CN113444789A (zh) * | 2021-08-27 | 2021-09-28 | 中国医学科学院北京协和医院 | 青光眼相关生物标志物及其应用 |
| CN114965664A (zh) * | 2022-03-21 | 2022-08-30 | 上海交通大学 | 一种获取泪液代谢指纹谱图的方法及筛选方法 |
| CN118425510B (zh) * | 2024-05-17 | 2024-11-19 | 上海市第一人民医院 | 一种青光眼诊断的生物标志物及其应用 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9419267D0 (en) | 1994-09-23 | 1994-11-09 | Unilever Plc | Assay devices |
| RU2187983C2 (ru) | 1998-11-10 | 2002-08-27 | Государственное учреждение Межотраслевой научно-технический комплекс "Микрохирургия глаза" | Способ диагностики характера течения глаукомы |
| US20030149997A1 (en) | 1999-02-19 | 2003-08-07 | Hageman Gregory S. | Diagnostics and therapeutics for arterial wall disruptive disorders |
| US20020102581A1 (en) * | 1999-02-19 | 2002-08-01 | Hageman Gregory S. | Diagnostics and therapeutics for ocular disorders |
| US7011952B2 (en) * | 2000-02-22 | 2006-03-14 | University Of Iowa Research Foundation | Diagnostics and therapeutics for macular degeneration-related disorders |
| AU2001261103B2 (en) * | 2000-04-29 | 2007-08-23 | University Of Iowa Research Foundation | Diagnostics and therapeutics for macular degeneration-related disorders |
| CN1393470A (zh) * | 2001-06-29 | 2003-01-29 | 上海博德基因开发有限公司 | 一种多肽——beta转导素－45.65和编码这种多肽的多核苷酸 |
| WO2003049773A1 (en) * | 2001-12-11 | 2003-06-19 | Fibrogen, Inc. | Methods for inhibiting ocular processes |
| JP2006506970A (ja) * | 2002-07-12 | 2006-03-02 | ユニバーシティ オブ ワシントン | 神経細胞の延長されたインビトロ培養のための方法および系 |
| US20060166268A1 (en) * | 2002-10-18 | 2006-07-27 | Rescom Gmbh | Diagnosis of glaucoma by complex autoantibody repertoires in body fluids |
| US7662389B2 (en) * | 2003-12-17 | 2010-02-16 | Alcon, Inc. | Use of serum amyloid A gene in diagnosis and treatment of glaucoma and identification of anti-glaucoma agents |
| US7442720B2 (en) | 2003-12-19 | 2008-10-28 | Omega Bio-Pharma (I.P.3) Limited | Compositions and methods for treating diabetes |
| AU2006272497B2 (en) * | 2005-07-27 | 2012-07-19 | University Of Florida Research Foundation, Inc. | Small compounds that correct protein misfolding and uses thereof |
| EP1832600A1 (en) | 2006-03-09 | 2007-09-12 | Max-Delbrück-Centrum Für Molekulare Medizin | Peptides against autoantibodies associated with glaucoma and use of these peptides |
| RU2314535C1 (ru) | 2006-07-05 | 2008-01-10 | Федеральное государственное учреждение "Межотраслевой научно-технический комплекс "Микрохирургия глаза" имени академика С.Н. Федорова Федерального агентства по здравоохранению и социальному развитию" | Способ диагностики начальной стадии открытоугольной глаукомы |
| KR20090034961A (ko) * | 2006-07-08 | 2009-04-08 | 유니버시티 오브 켄터키 리서치 파운데이션 | 폐암 진단 검사 |
| EP1929937A1 (de) | 2006-12-07 | 2008-06-11 | F. Hoffmann-Roche AG | Vorrichtung und Verfahren zum Untersuchen von Körperflüssigkeiten |
| RU2371721C2 (ru) | 2007-06-25 | 2009-10-27 | Закрытое акционерное общество "Молекулярно-медицинские технологии" | Устройство для диагностики с использованием биочипов |
| GB0724412D0 (en) * | 2007-12-14 | 2008-02-06 | Ucl Business Plc | Marker |
| KR101188439B1 (ko) | 2008-04-02 | 2012-10-08 | (주)루미아이제네틱스 | 녹내장 진단용 키트 |
| EP2354792A1 (en) | 2010-02-08 | 2011-08-10 | Biomonitor A/S | Method for detecting anti-drug antibodies |
| KR20100087276A (ko) | 2010-07-21 | 2010-08-04 | (주)루미아이제네틱스 | 녹내장 진단용 키트 |
| KR20100087275A (ko) | 2010-07-21 | 2010-08-04 | (주)루미아이제네틱스 | 녹내장 진단용 키트 |
-
2011
- 2011-04-13 JP JP2013504084A patent/JP2013529292A/ja active Pending
- 2011-04-13 EP EP11714916.1A patent/EP2558862B1/en active Active
- 2011-04-13 CA CA2795270A patent/CA2795270C/en active Active
- 2011-04-13 DK DK11714916.1T patent/DK2558862T3/en active
- 2011-04-13 BR BR112012026219A patent/BR112012026219B1/pt active IP Right Grant
- 2011-04-13 PL PL11714916T patent/PL2558862T3/pl unknown
- 2011-04-13 US US13/641,009 patent/US10175251B2/en active Active
- 2011-04-13 RU RU2012147551A patent/RU2657515C2/ru active
- 2011-04-13 CN CN201180028608.XA patent/CN103003693B/zh active Active
- 2011-04-13 AU AU2011241402A patent/AU2011241402B2/en active Active
- 2011-04-13 TR TR2019/02542T patent/TR201902542T4/tr unknown
- 2011-04-13 HR HRP20190278TT patent/HRP20190278T1/hr unknown
- 2011-04-13 ES ES11714916T patent/ES2712060T3/es active Active
- 2011-04-13 WO PCT/CH2011/000077 patent/WO2011127616A1/en not_active Ceased
- 2011-04-13 KR KR1020127029751A patent/KR101903193B1/ko active Active
- 2011-04-13 HU HUE11714916A patent/HUE042703T2/hu unknown
-
2012
- 2012-10-22 ZA ZA2012/07935A patent/ZA201207935B/en unknown
-
2015
- 2015-12-25 JP JP2015253808A patent/JP6225157B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR20130122706A (ko) | 2013-11-08 |
| DK2558862T3 (en) | 2019-03-18 |
| JP6225157B2 (ja) | 2017-11-01 |
| CN103003693B (zh) | 2016-07-06 |
| US20130172204A1 (en) | 2013-07-04 |
| RU2012147551A (ru) | 2014-05-20 |
| BR112012026219A2 (pt) | 2016-07-12 |
| AU2011241402A1 (en) | 2012-11-15 |
| EP2558862B1 (en) | 2018-11-21 |
| ES2712060T3 (es) | 2019-05-09 |
| JP2013529292A (ja) | 2013-07-18 |
| PL2558862T3 (pl) | 2019-07-31 |
| CN103003693A (zh) | 2013-03-27 |
| CA2795270A1 (en) | 2011-10-20 |
| ZA201207935B (en) | 2019-07-31 |
| EP2558862A1 (en) | 2013-02-20 |
| KR101903193B1 (ko) | 2018-10-01 |
| JP2016048265A (ja) | 2016-04-07 |
| TR201902542T4 (tr) | 2019-03-21 |
| AU2011241402B2 (en) | 2016-09-15 |
| WO2011127616A1 (en) | 2011-10-20 |
| HUE042703T2 (hu) | 2019-07-29 |
| US10175251B2 (en) | 2019-01-08 |
| HRP20190278T1 (hr) | 2019-04-19 |
| RU2657515C2 (ru) | 2018-06-14 |
| CA2795270C (en) | 2021-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112012026219B1 (pt) | método para o diagnóstico de glaucoma, elementos carreadores de antígeno e kit | |
| Beutgen et al. | Autoantibody biomarker discovery in primary open angle glaucoma using serological proteome analysis (SERPA) | |
| US20170176460A1 (en) | Neural specific s100-beta for biomarker assays and devices for detection of a neurological condition | |
| IL292342A (en) | Biomarkers and their uses for the diagnosis of the silent phase in Alzheimer's disease | |
| CN105008923B (zh) | 在癌症诊断中的血小板生物标记物 | |
| Soria et al. | The analysis of human conjunctival epithelium proteome in ocular surface diseases using impression cytology and 2D-DIGE | |
| US20140024053A1 (en) | Methods, kits and devices for detecting bii-spectrin, and breakdown products thereof, as biomarkers for the diagnosis of neural injury | |
| EP2444814B1 (en) | Biomarker for mental disorders including cognitive disorders, and method using said biomarker to detect mental disorders including cognitive disorders | |
| WO2010101047A1 (ja) | 子宮内膜症の判定方法、および子宮内膜症の診断用キット | |
| US9977036B2 (en) | Diagnostic markers for multiple sclerosis | |
| CN115398239A (zh) | 帕金森氏病诊断用生物标记物及利用其的帕金森氏病诊断方法 | |
| CN108431608A (zh) | 用于诊断或预后阿尔茨海默病或用于诊断帕金森病的乳铁蛋白 | |
| EP2603797B1 (en) | Method for the diagnosis of dry eye and blepharitis | |
| WO2012004276A2 (en) | Multiprotein biomarkers of amyotrophic lateral sclerosis in peripheral blood mononuclear cells, diagnostic methods and kits | |
| Banerjee et al. | Isobaric tagging-based quantification by mass spectrometry of differentially regulated proteins in synaptosomes of HIV/gp120 transgenic mice: implications for HIV-associated neurodegeneration | |
| ES2781195T3 (es) | Método para el diagnóstico de artrosis | |
| WO2019169309A1 (en) | Methods, apparatuses and kits for rapid testing of traumatic brain injuries | |
| Zill et al. | Postmortem proteomic analysis in human amygdala of drug addicts: possible impact of tubulin on drug-abusing behavior | |
| RU2741227C1 (ru) | Способ диагностики черепно-мозговой травмы с использованием белковых биомаркеров | |
| BR102023020955A2 (pt) | Me´todo de identificac¸a~o de biomarcadores para a predic¸a~o e monitoramento de doenc¸as oftalmolo´gicas, kit para identificac¸a~o de biomarcadores e usos | |
| WO2025076604A1 (pt) | Método de identificação de biomarcadores para a predição e monitoramento de doenças oftalmológicas, kit para identificação de biomarcadores e usos | |
| TW202111324A (zh) | Lama2, plxdc2 及 mll4 作為糖尿病前期和糖尿病的新型生物標記 | |
| JP2021076410A (ja) | 肺がん罹患のリスクを評価するための方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B25G | Requested change of headquarter approved |
Owner name: M-LAB AG (CH) |
|
| B25A | Requested transfer of rights approved |
Owner name: M-LAB GMBH (DE) |
|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06T | Formal requirements before examination [chapter 6.20 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 13/04/2011, OBSERVADAS AS CONDICOES LEGAIS. (CO) 20 (VINTE) ANOS CONTADOS A PARTIR DE 13/04/2011, OBSERVADAS AS CONDICOES LEGAIS |