BR112012023875A2 - uso de acromopeptidase para lise em temperatura ambiente - Google Patents
uso de acromopeptidase para lise em temperatura ambienteInfo
- Publication number
- BR112012023875A2 BR112012023875A2 BR112012023875A BR112012023875A BR112012023875A2 BR 112012023875 A2 BR112012023875 A2 BR 112012023875A2 BR 112012023875 A BR112012023875 A BR 112012023875A BR 112012023875 A BR112012023875 A BR 112012023875A BR 112012023875 A2 BR112012023875 A2 BR 112012023875A2
- Authority
- BR
- Brazil
- Prior art keywords
- acromopeptidase
- room temperature
- sample
- lysis
- absence
- Prior art date
Links
- 230000009089 cytolysis Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 241000192125 Firmicutes Species 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 2
- 230000001413 cellular effect Effects 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000124008 Mammalia Species 0.000 abstract 1
- 241000191967 Staphylococcus aureus Species 0.000 abstract 1
- 241000193985 Streptococcus agalactiae Species 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 abstract 1
- 230000002934 lysing effect Effects 0.000 abstract 1
- NDLPOXTZKUMGOV-UHFFFAOYSA-N oxo(oxoferriooxy)iron hydrate Chemical compound O.O=[Fe]O[Fe]=O NDLPOXTZKUMGOV-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31431810P | 2010-03-16 | 2010-03-16 | |
| PCT/US2011/028494 WO2011115975A2 (en) | 2010-03-16 | 2011-03-15 | Use of achromopeptidase for lysis at room temperature |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112012023875A2 true BR112012023875A2 (pt) | 2015-09-22 |
Family
ID=44649786
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112012023875A BR112012023875A2 (pt) | 2010-03-16 | 2011-03-15 | uso de acromopeptidase para lise em temperatura ambiente |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20110275090A1 (https=) |
| EP (1) | EP2547767A4 (https=) |
| JP (1) | JP2013521804A (https=) |
| CN (1) | CN102869773A (https=) |
| AU (1) | AU2011227452B2 (https=) |
| BR (1) | BR112012023875A2 (https=) |
| CA (1) | CA2793144A1 (https=) |
| WO (1) | WO2011115975A2 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2502761B (en) | 2011-03-09 | 2015-09-30 | Becton Dickinson Infusion Therapy Systems Inc | Method and internal control composition for determining the efficacy of enzymatic cell lysis |
| US9528987B2 (en) | 2011-06-23 | 2016-12-27 | University Of Washington | Reagent patterning in capillarity-based analyzers and associated systems and methods |
| WO2013117968A1 (en) | 2012-02-10 | 2013-08-15 | Reametrix Inc. | Methods for lysing bacteria from sample and isolating cellular components therefrom, and kits therefor |
| CN105050720A (zh) | 2013-01-22 | 2015-11-11 | 华盛顿大学商业化中心 | 顺序递送流体体积和相关的设备、系统和方法 |
| EP3320966B1 (en) | 2016-11-14 | 2022-02-16 | Airbus Operations GmbH | Autoclave and method for welding thermoplastic composite parts |
| EP3681895A4 (en) * | 2017-09-13 | 2021-05-26 | Becton, Dickinson and Company | METHODS AND COMPOSITIONS FOR EXTRACTION OF NUCLEIC ACIDS USING FERROUS PARTICLES |
| WO2021217173A1 (en) * | 2020-04-21 | 2021-10-28 | Siemens Healthcare Diagnostics Inc. | Clinical specimen collection swabs for molecular diagnostic applications and methods of production and use thereof |
| CN118834934B (zh) * | 2024-08-19 | 2025-05-16 | 希莱乐检(郑州)生物科技有限公司 | 用于结核分枝杆菌核酸释放的裂解液及其应用 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8414273D0 (en) * | 1984-06-05 | 1984-07-11 | Oxoid Ltd | Identifying streptococcal grouping |
| JP2589729B2 (ja) * | 1988-01-30 | 1997-03-12 | 極東製薬工業株式会社 | 染色体ｄｎａを用いた細菌の同定方法および該同定用キット |
| JPH06510363A (ja) * | 1990-10-29 | 1994-11-17 | ディカルブ プラント ジェネティクス | 磁気性粒子を使用する生物学的材料の単離 |
| US5185242A (en) * | 1991-06-24 | 1993-02-09 | Becton Dickinson And Company | Method for lysing mycobacteria using achromopeptidase |
| US5973138A (en) * | 1998-10-30 | 1999-10-26 | Becton Dickinson And Company | Method for purification and manipulation of nucleic acids using paramagnetic particles |
| JP3172917B2 (ja) * | 1999-07-23 | 2001-06-04 | 北海道 | 細菌検出方法 |
| US20040002594A1 (en) * | 2002-06-27 | 2004-01-01 | John Krupey | Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids |
| WO2004108965A1 (en) * | 2003-05-30 | 2004-12-16 | Georgia Tech Research Corporation | Methods and reagents for quantitative analysis of dehalococcoides species |
| WO2005064016A1 (ja) * | 2003-12-26 | 2005-07-14 | Prima Meat Packers, Ltd. | 微生物の多重検出方法 |
| US20060057613A1 (en) * | 2004-07-26 | 2006-03-16 | Nanosphere, Inc. | Method for distinguishing methicillin resistant S. aureus from methicillin sensitive S. aureus in a mixed culture |
| KR100988668B1 (ko) * | 2005-07-21 | 2010-10-18 | 모리나가 뉴교 가부시키가이샤 | 미생물 검출법 및 미생물 검출 키트 |
| US20100099115A1 (en) * | 2006-11-22 | 2010-04-22 | Mach Patrick A | Systems and methods for preparing and analyzing samples |
| JP2008148570A (ja) * | 2006-12-14 | 2008-07-03 | Hitachi Ltd | 微生物検出システム |
| US20090215050A1 (en) * | 2008-02-22 | 2009-08-27 | Robert Delmar Jenison | Systems and methods for point-of-care amplification and detection of polynucleotides |
| US20090274577A1 (en) * | 2008-05-05 | 2009-11-05 | B-K Medical Aps | Sterilization by treatment with reductant and oxidant |
-
2011
- 2011-03-15 CA CA2793144A patent/CA2793144A1/en not_active Abandoned
- 2011-03-15 BR BR112012023875A patent/BR112012023875A2/pt not_active Application Discontinuation
- 2011-03-15 EP EP11756845.1A patent/EP2547767A4/en not_active Withdrawn
- 2011-03-15 AU AU2011227452A patent/AU2011227452B2/en not_active Ceased
- 2011-03-15 US US13/048,539 patent/US20110275090A1/en not_active Abandoned
- 2011-03-15 CN CN2011800203890A patent/CN102869773A/zh active Pending
- 2011-03-15 JP JP2013500151A patent/JP2013521804A/ja active Pending
- 2011-03-15 WO PCT/US2011/028494 patent/WO2011115975A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP2547767A4 (en) | 2013-12-25 |
| WO2011115975A2 (en) | 2011-09-22 |
| AU2011227452B2 (en) | 2015-04-09 |
| JP2013521804A (ja) | 2013-06-13 |
| US20110275090A1 (en) | 2011-11-10 |
| WO2011115975A3 (en) | 2012-01-19 |
| AU2011227452A1 (en) | 2012-10-04 |
| CA2793144A1 (en) | 2011-09-22 |
| CN102869773A (zh) | 2013-01-09 |
| EP2547767A2 (en) | 2013-01-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112012023875A2 (pt) | uso de acromopeptidase para lise em temperatura ambiente | |
| WO2015058008A3 (en) | Enhanced nucleic acid identification and detection | |
| WO2014005125A3 (en) | Fluorescent compounds and uses thereof | |
| BR112014020512A8 (pt) | Métodos e sistemas para a detecção de microorganismos | |
| BR112014030298A2 (pt) | ensaios multiplexados baseados em aptâmero | |
| BR112015008708A2 (pt) | composição para clivagem de dna alvo compreendendo um rna guia específico para o dna alvo e ácido nucleico que codifica proteína cas ou proteína cas e uso da mesma | |
| EP4397767A3 (en) | Microcapsule compositions and methods | |
| PH12016500300A1 (en) | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples | |
| WO2012042374A3 (en) | Method of determining number or concentration of molecules | |
| WO2009135158A3 (en) | Detection of microbial nucleic acids | |
| BR112012030813A2 (pt) | "métodos e kits para detecção in situ de sequências de nucleotídeo" | |
| GB201017978D0 (en) | Multiplex amplification and detection | |
| BR112017025785A2 (pt) | ?biossensor, enzima de glicose desidrogenase, enzima oxirredutase, polipeptídeo, composição ou kit, método de detecção de uma molécula alvo, método de diagnóstico de uma doença ou condição em um organismo, dispositivo de detecção, ácido nucleico isolado, construção gênica, célula hospedeira e método para produzir um biossensor? | |
| EP3543362A3 (en) | Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species | |
| WO2014018958A3 (en) | Methods and devices for electromagnetic amplification of nucleic acids | |
| BR112015026707A2 (pt) | Proteína ppr, e, complexo | |
| WO2014003583A3 (en) | Detection of pathogens | |
| WO2012064978A3 (en) | Compositions, methods, and kits for detecting and identifying mycobacteria | |
| WO2018128706A3 (en) | Modified nucleic acids for nanopore analysis | |
| WO2018111630A3 (en) | Rnase for improved microbial detection and antimicrobial susceptibility testing | |
| HK1220250A1 (zh) | 微生物测定方法 | |
| WO2013126793A3 (en) | Detection of shiga toxin genes in bacteria | |
| WO2012034130A3 (en) | Methods and compositions for nucleic acid detection | |
| JP2013521804A5 (https=) | ||
| BR112016010159A2 (pt) | usos de um conjunto de ácidos nucleicos e de um chip de microarranjo, chip de microarranjo, métodos para detecção de patógenos e/ou microrganismos benéficos em uma amostra orgânica e de tratamento de uma relva doente, e, kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B15K | Others concerning applications: alteration of classification |
Ipc: C12N 15/10 (2006.01) |
|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07A | Technical examination (opinion): publication of technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL |