BG65693B1 - Biologically active plant product and method of obtaining it - Google Patents

Abstract

The invention relates to a biologically active plant product comprising dry extract from Cichoria intybus and/or Cotinus coggygria and/or Tanacetum vulgare alone or in the form of a homogenous mixture, as well as to a method of obtaining it. The high levels of physiologically active substances in the product make it possible to use it as an additive in forage and drinking water and as a substitute for nutritive antibiotics in livestock breeding.

Description

Technical field

The invention relates to a plant biologically active product and a method for its preparation for animal husbandry.

BACKGROUND OF THE INVENTION

Methods for the preparation of biologically active products from various plant materials are known, which involve extraction with water or aqueous-ethanol mixtures in which the amount of water is predominant - from 65 to 80% (EP 1965817). The products obtained are total extracts containing an uncontrolled amount of active ingredients and ballast substances and therefore cannot be used depending on the physiological orientation of the active ingredients.

EP 1978980 describes a method for preparing a plant extract from Rosa sp., Urtica dioica and Tanacetum vulgare with ethanol for the preparation of a medicament for immunostimulation and / or treatment of HTV infection. The method envisages additional enrichment of the obtained extract with selenium and urea and exposure with an electromagnetic field. The disadvantage of the product obtained is that the vegetable extract is preferably carried out with 96% ethanol, which ensures the extraction of the poisonous substance thujone from the Tanacetum vulgare herb, but both in the description and in the claims the ingredients in the final product resulting from the the method used has not been identified. Respectively, the claimed effect of the application of the plant extract is unproven.

SUMMARY OF THE INVENTION

The present invention is intended to provide a plant biologically active product with well-defined characteristics and a method for its preparation for animal husbandry.

In essence, the plant biologically active product of the invention is a homogeneous mixture of dried extracts of Cichoria intybus and / or Cotinus coggygria and / or Tanacetum vulgare plants.

The process for preparing the plant biologically active product as a homogeneous mixture of dry extracts involves treating the raw material with chloroform and extracting the biologically active substances with ethanol, followed by filtration, concentration and quenching for 7 to 12 hours, after which the separated ballast substances are filtered off and the purified concentrates were dried and mixed in the desired combination.

In particular, milled drug from Cichoria intybus or Cotinus coggygria or Tanacetum vulgare is treated at a temperature of 30 to 40 ° C with chloroform in a ratio of 1: 5 to 1:10 for degreasing purposes, the chloroform being regenerated and reused. For the extraction of physiologically active substances, the degreased drug is extracted with a water-ethanol solution containing from 60 to 85% ethanol, using a 75% ethanol solution for the extraction of flavonoids, coumarins and vegetable ketones, and for the extraction of tannins and saponins - 65% ethanol solution. The extraction is carried out at a temperature of 30 to 60 ° C, and the extractants after cooling are filtered, concentrated and left to stand for 7 to 12 hours. The separated ballast substances are filtered off and the purified concentrates are dried. The dry extracts thus obtained are used alone or mixed in the appropriate desired combination and homogenized for 45 minutes to give a homogeneous mixture.

The herbal biologically active product according to the invention is standardized in terms of flavonoids, tannins and coumarin derivatives. Flavonoids, defined as apigenin, rutin, quartzetin, campferol, or isorhamnetin, have anti-inflammatory and anti-allergic effects; tannins, identified as tannin or pyrogalol, affect the intestinal mucosa, inhibit the development of putrefactive microorganisms, exhibiting antiseptic and astringent action; coumarin derivatives defined as chicoryn, escu2

65693 Bl lentin or coumarin, hepatoprotectively affect the liver parenchyma and protect it from the effects of toxic substances.

Based on the content of the above physiologically active substances, the plant biologically active product of the present invention is useful as an adjunct to feed and drinking water for the breeding of broiler chickens and laying hens for feeding pigs, rabbits and the like. and as a substitute for nutritional antibiotics in animal husbandry.

The invention is illustrated by the following exemplary embodiments without limiting its scope.

Example 1. Preparation of a Plant Bioactive Product

50 g of ground drug from the plant Cichoria intybus or Cotinus coggygria or Tanacetum vulgare are placed in a laboratory reactor. 250 ml of chloroform was added and stirred at 35 ° C for 60 min.

The degreased drug is extracted with 75% aqueous ethanol solution, concentrated to 1/3 of volume, allowed to stand for 10 h, filtered and the filtrate dried. The last 5 is used both individually and as a dry homogeneous mixture with the other two herbs treated by the same method.

Example 2. Biologically active product as a combination of three plant extracts

400 g of Cichoria intybus dry extract, 300 g of Cotinus coggygria dry extract and 300 g of Tanacetum vulgare dry extract are placed in a laboratory homogenizer. The mixture was stirred for 45 min.

Table 1 below shows the tannin content, calculated as tannin, flavonoids, defined as apigenin, and coumarin derivatives, defined as coumarin, which predetermine antimicrobial, anti-helminthic and hepatoprotective properties, and the present product biome content 2.

Table 1

A herbal biologically active product of the invention Physiologically active substances (%) Tanning agents Flavonoids Coumarins Seskiterpene lacgons Tannin Apigenin Coumarin Santonin Cichoria intybus dry extract 7.3 9.5 9.5 17.0 Dry extract of Cotinus coggygria 32.0 25.9 3.0 1.0 Dry extract of Tanacetum vulgare 11.0 18.8 1.5 4.5 A homogeneous mixture of dry extracts according to Example 2 16.0 17.0 4.2 7.2

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Example 3. Biologically active product as a combination of two plant extracts

Put in a laboratory homogenizer 500 g of dry extract of Cichoria intybus and 500 g of dry extract of Cotinus coggygria. The mixture was stirred for 45 min. The data obtained from feeding the broiler chickens with the product according to Example 3 of the present invention are set out in Table 2.

Table 2

Indicators 1 group Control Group II + biologically active product according to Example 3 Live weight, e (%) on day 28 on day 42 828 (100) 1855 (100) 877 (106.5) 1952 (105.2) Feed consumption per 1 kg gain -1 to 42 days, kg (%) 2,072 (100) 1,923 (92.8)

65693 Bl

Example 4. Biologically active product as a combination of two plant extracts

Put in a laboratory homogenizer 500 g of dry extract of Cichoria intybus and 500 g of dry extract of Tanacetum vulgare. The mixture was stirred for 45 min. Data obtained from feeding pigs and maternal pigs with the product according to Example 4 of the present invention are set out in Table 3.

Table 3

Groups 1 Indicators Group I Control Group II + Biologically active product according to Example 4. Number of mothers 5 5 Fodder accepted, cd 100-114 days 2.5 2.5 1 - 35days 6.0 6.108 Number of pigs 41 37 Live birth, kD at birth 1.432 ± 0.02® 1.502 ± 0.026® on day 21 1.777 ± 0.118® 5.189 ± 0.162® on day 35 6.788 ± 0.175 7.248 ± 0.335 Growth, d / day 1-21 days 159 ± 5.83 176 ± 7.73 22-35days 144 ± 10.56 147 ± 15.76 1-35 days 152 ± 4.98 164 ± 9.47

Example 5. Use as a substitute for nutrient antibiotics in animal husbandry

The test results of the biologically inactive product of the present invention, as a substitute for nutritive antibiotics, are shown in Table 4.

Table 4

Groups / Indicators Control with Flavomycin Control without antibiotic Experienced with a biologically active product according to Example 2 Number of pigs 10 (2x5) 10 (2x5) 10 (2x5) Drop out, pc. 2 2 1 Period, days 60 60 60 Live weight, at stake 7.16 7.19 7.3 On completion 18.61 17.68 20.90 Poioast - e / day ^b 191® 175® ° 226 ^Yo -% 100.0 91.6 118.3 100.0 129.1 Cost of (bvoax accepted / day / pig e 496 523 543 for 1k increment, cd 2.599 ° 2.995 °° 2.398 ° % of control 100.0 115.2 92.2 100.0 80.1

Claims

Claims (5)

A herbal biologically active product based on dried herbal extracts, characterized in that it comprises a dried extract of Cichoria intybus and / or Cotinus coggygria and / or Tanacetum vulgare, alone or in the form of a homogeneous mixture. A method for the preparation of a plant biologically active product according to claim 1, characterized in that the milled drug from Cichoria intybus or Cotinus coggygria or Tanacetum vulgare is treated with chloroform in a ratio of 1: 5 to 1:10, ethanol extraction is carried out, the extracts obtained are filtered, concentrated and allowed to stand 65693 Bl 7 to 12 h, after which the separated ballast substances are filtered off and the purified concentrates are dried. A process for the preparation of a plant bioactive product according to claim 2, characterized in that the ethanol extraction is carried out with an aqueous-ethanol solution containing from 60 to 85% ethanol. A method for preparing a plant biologically active product according to claims 2 and 3, characterized in that the ethanol extraction is carried out at a temperature of from 30 to 60 ° C. 5. Use of a herbal biologically active product according to claims 1 to 4 for the production of a substitute for nutritional antibiotics in animal husbandry.