BE751362A - Placentary lactogen preparation - Google Patents
Placentary lactogen preparationInfo
- Publication number
- BE751362A BE751362A BE751362DA BE751362A BE 751362 A BE751362 A BE 751362A BE 751362D A BE751362D A BE 751362DA BE 751362 A BE751362 A BE 751362A
- Authority
- BE
- Belgium
- Prior art keywords
- lactogen
- preparation
- placental lactogen
- nh4hco3
- sephadex
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pregnancy & Childbirth (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Preparation of placental lactogen by (a) treating frozen human placenta in order to extract the proteins and the placental hormone; (b) precipitating the hormone from the solution eluted from an ion-exchanger by increasing the ionic strength with (NH4)2SO4; (c) suspending the precipitate in NH4HCO3 and passing through a column of 'Sephadex' C25 (RTM) and a column of 'Sephadex' G100 buffered with NH4HCO3 in order to eliminate (NH4)2SO4 and to equilibrate the protein; (d) eliminating higher molecular impurities by recycling; and (e) precipitating the resulting placental lactogen with (NH4)2SO4, dialysing in the presence of H2O and lyophilizing. Placental lactogen is useful inter alia for diagnostic purposes.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT461869 | 1969-06-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
BE751362A true BE751362A (en) | 1970-11-16 |
Family
ID=11112787
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BE751362D BE751362A (en) | 1969-06-03 | 1970-06-02 | Placentary lactogen preparation |
Country Status (1)
Country | Link |
---|---|
BE (1) | BE751362A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2413363A1 (en) * | 1977-12-27 | 1979-07-27 | Sclavo Inst Sieroterapeut | METHOD FOR THE PREPARATION OF HUMAN CHORIONIC SOMATOMAMMOTROPIN |
-
1970
- 1970-06-02 BE BE751362D patent/BE751362A/en unknown
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2413363A1 (en) * | 1977-12-27 | 1979-07-27 | Sclavo Inst Sieroterapeut | METHOD FOR THE PREPARATION OF HUMAN CHORIONIC SOMATOMAMMOTROPIN |
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