BE751362A - Placentary lactogen preparation - Google Patents

Placentary lactogen preparation

Info

Publication number
BE751362A
BE751362A BE751362DA BE751362A BE 751362 A BE751362 A BE 751362A BE 751362D A BE751362D A BE 751362DA BE 751362 A BE751362 A BE 751362A
Authority
BE
Belgium
Prior art keywords
lactogen
preparation
placental lactogen
nh4hco3
sephadex
Prior art date
Application number
Other languages
French (fr)
Original Assignee
Sclavo Inst Sieroterapeut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sclavo Inst Sieroterapeut filed Critical Sclavo Inst Sieroterapeut
Publication of BE751362A publication Critical patent/BE751362A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57518—Placental lactogen; Chorionic somatomammotropin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pregnancy & Childbirth (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Preparation of placental lactogen by (a) treating frozen human placenta in order to extract the proteins and the placental hormone; (b) precipitating the hormone from the solution eluted from an ion-exchanger by increasing the ionic strength with (NH4)2SO4; (c) suspending the precipitate in NH4HCO3 and passing through a column of 'Sephadex' C25 (RTM) and a column of 'Sephadex' G100 buffered with NH4HCO3 in order to eliminate (NH4)2SO4 and to equilibrate the protein; (d) eliminating higher molecular impurities by recycling; and (e) precipitating the resulting placental lactogen with (NH4)2SO4, dialysing in the presence of H2O and lyophilizing. Placental lactogen is useful inter alia for diagnostic purposes.
BE751362D 1969-06-03 1970-06-02 Placentary lactogen preparation BE751362A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT461869 1969-06-03

Publications (1)

Publication Number Publication Date
BE751362A true BE751362A (en) 1970-11-16

Family

ID=11112787

Family Applications (1)

Application Number Title Priority Date Filing Date
BE751362D BE751362A (en) 1969-06-03 1970-06-02 Placentary lactogen preparation

Country Status (1)

Country Link
BE (1) BE751362A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2413363A1 (en) * 1977-12-27 1979-07-27 Sclavo Inst Sieroterapeut METHOD FOR THE PREPARATION OF HUMAN CHORIONIC SOMATOMAMMOTROPIN

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2413363A1 (en) * 1977-12-27 1979-07-27 Sclavo Inst Sieroterapeut METHOD FOR THE PREPARATION OF HUMAN CHORIONIC SOMATOMAMMOTROPIN

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