GB1388719A - Pure t-globulin and its anti-serum - Google Patents

Pure t-globulin and its anti-serum

Info

Publication number
GB1388719A
GB1388719A GB4059572A GB4059572A GB1388719A GB 1388719 A GB1388719 A GB 1388719A GB 4059572 A GB4059572 A GB 4059572A GB 4059572 A GB4059572 A GB 4059572A GB 1388719 A GB1388719 A GB 1388719A
Authority
GB
United Kingdom
Prior art keywords
globulin
precipitate
serum
electrophoresis
sept
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4059572A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HADASSAH MEDICAL RELIEF ASS IN
Original Assignee
HADASSAH MEDICAL RELIEF ASS IN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HADASSAH MEDICAL RELIEF ASS IN filed Critical HADASSAH MEDICAL RELIEF ASS IN
Publication of GB1388719A publication Critical patent/GB1388719A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

1388719 T-globulin and anti-serum therefor HADASSAN MEDICAL RELIEF ASSOCIATION Inc 1 Sept 1972 [2 Sept 1971] 40595/72 Heading A5B [Also in Division G1] The invention comprises pure T-globulin, i.e. a globulin fraction of M.W. 115,000-170,000 detectable in the blood of pregnant women and cancer sufferers, which when precipitated from serum and subjected to electrophoresis as below migrates towards the positive electrode at 5mm/hr. The electrophoresis is carried out in an agar block measuring 75mm (between the electrodes) x 25mm x 4mm, the current is 10mA, the voltage gradient is 4.5 volts/cm and the agar is buffered at pH 8.6. In preparing T-globulin, blood serum from cancer-sufferers and for pregnant women is treated with saturated ammonium sulphate to precipitate the globulin, from which the appropriate molecular weight fraction is collected as follows: (a) The globulin is dissolved in water (b) a precipitate is formed by addition of perchloric acid, (c) the precipitate is washed with neutral saline, (d) the precipitate is extracted with saline at pH 7.8-8.2 to dissolve out the T- globulin. T-globulin anti serum may be prepared by injecting T-globulin and Freund's complete adjuvant into a mammal. The antiserum may be purified by electrophoresis or by precipitation with perchloric acid followed by re-solution at pH 7.8-8.2. It may further be purified by treating it with normal blood serum to precipitate out all the anti-bodies except anti-T-globulin.
GB4059572A 1971-09-02 1972-09-01 Pure t-globulin and its anti-serum Expired GB1388719A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US17723971A 1971-09-02 1971-09-02

Publications (1)

Publication Number Publication Date
GB1388719A true GB1388719A (en) 1975-03-26

Family

ID=22647782

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4059572A Expired GB1388719A (en) 1971-09-02 1972-09-01 Pure t-globulin and its anti-serum

Country Status (4)

Country Link
JP (1) JPS4835028A (en)
DE (1) DE2243237A1 (en)
FR (1) FR2161896B1 (en)
GB (1) GB1388719A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2408339A1 (en) * 1977-11-11 1979-06-08 Behringwerke Ag METHOD FOR DIAGNOSING MALIGNANT TUMORS USING A SYNTHETIC PEPTIDE WITH HIGH BASICITY
US4234476A (en) 1978-03-07 1980-11-18 Research Corporation Application of protein-protein interaction as an assay for the detection of cancer

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2343250A1 (en) * 1976-03-03 1977-09-30 Anvar Test for T:antigens - using a specific antibody from an animal of a different species to that under test
JPS548714A (en) * 1977-06-16 1979-01-23 Saikin Kagaku Kenkiyuushiyo Kk Production of specific antiserum
JPS59159073A (en) * 1983-02-28 1984-09-08 Nec Home Electronics Ltd Clock apparatus

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2408339A1 (en) * 1977-11-11 1979-06-08 Behringwerke Ag METHOD FOR DIAGNOSING MALIGNANT TUMORS USING A SYNTHETIC PEPTIDE WITH HIGH BASICITY
US4234476A (en) 1978-03-07 1980-11-18 Research Corporation Application of protein-protein interaction as an assay for the detection of cancer

Also Published As

Publication number Publication date
DE2243237A1 (en) 1973-03-08
JPS4835028A (en) 1973-05-23
FR2161896B1 (en) 1976-10-29
FR2161896A1 (en) 1973-07-13

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Legal Events

Date Code Title Description
PS Patent sealed
PLNP Patent lapsed through nonpayment of renewal fees