AU785148B2 - Use of recombinant parainfluenza viruses (PIVs) as vectors to protect against infection and disease caused by PIV and other human pathogens - Google Patents
Use of recombinant parainfluenza viruses (PIVs) as vectors to protect against infection and disease caused by PIV and other human pathogens Download PDFInfo
- Publication number
- AU785148B2 AU785148B2 AU20731/01A AU2073101A AU785148B2 AU 785148 B2 AU785148 B2 AU 785148B2 AU 20731/01 A AU20731/01 A AU 20731/01A AU 2073101 A AU2073101 A AU 2073101A AU 785148 B2 AU785148 B2 AU 785148B2
- Authority
- AU
- Australia
- Prior art keywords
- piv
- genome
- chimeric
- antigenome
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 239000013598 vector Substances 0.000 title claims description 353
- 241000700605 Viruses Species 0.000 title claims description 340
- 208000015181 infectious disease Diseases 0.000 title claims description 149
- 208000002606 Paramyxoviridae Infections Diseases 0.000 title claims description 32
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title description 22
- 201000010099 disease Diseases 0.000 title description 21
- 244000052637 human pathogen Species 0.000 title description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 643
- 230000035772 mutation Effects 0.000 claims description 239
- 230000000890 antigenic effect Effects 0.000 claims description 208
- 241000712079 Measles morbillivirus Species 0.000 claims description 202
- 108700026244 Open Reading Frames Proteins 0.000 claims description 199
- 102000004169 proteins and genes Human genes 0.000 claims description 174
- 101710133291 Hemagglutinin-neuraminidase Proteins 0.000 claims description 166
- 230000002238 attenuated effect Effects 0.000 claims description 147
- 244000052769 pathogen Species 0.000 claims description 140
- 108090000288 Glycoproteins Proteins 0.000 claims description 122
- 102000003886 Glycoproteins Human genes 0.000 claims description 117
- 230000036961 partial effect Effects 0.000 claims description 110
- 239000002773 nucleotide Substances 0.000 claims description 108
- 125000003729 nucleotide group Chemical group 0.000 claims description 107
- 108091033319 polynucleotide Proteins 0.000 claims description 107
- 239000002157 polynucleotide Substances 0.000 claims description 107
- 102000040430 polynucleotide Human genes 0.000 claims description 107
- 230000001717 pathogenic effect Effects 0.000 claims description 105
- 238000000034 method Methods 0.000 claims description 98
- 241000282414 Homo sapiens Species 0.000 claims description 96
- 239000012634 fragment Substances 0.000 claims description 89
- 238000003780 insertion Methods 0.000 claims description 89
- 230000037431 insertion Effects 0.000 claims description 89
- 241001559187 Human rubulavirus 2 Species 0.000 claims description 88
- 230000002458 infectious effect Effects 0.000 claims description 87
- 241000725643 Respiratory syncytial virus Species 0.000 claims description 86
- 230000002163 immunogen Effects 0.000 claims description 76
- 230000028993 immune response Effects 0.000 claims description 73
- 230000010076 replication Effects 0.000 claims description 72
- 238000006467 substitution reaction Methods 0.000 claims description 71
- 201000005505 Measles Diseases 0.000 claims description 56
- 239000000203 mixture Substances 0.000 claims description 56
- 210000002345 respiratory system Anatomy 0.000 claims description 55
- 230000014509 gene expression Effects 0.000 claims description 53
- 238000013518 transcription Methods 0.000 claims description 52
- 230000001681 protective effect Effects 0.000 claims description 51
- 230000035897 transcription Effects 0.000 claims description 51
- 150000001413 amino acids Chemical class 0.000 claims description 47
- 108090001074 Nucleocapsid Proteins Proteins 0.000 claims description 46
- 210000004027 cell Anatomy 0.000 claims description 45
- 239000002245 particle Substances 0.000 claims description 45
- 241000726041 Human respirovirus 1 Species 0.000 claims description 41
- 239000000427 antigen Substances 0.000 claims description 40
- 108091007433 antigens Proteins 0.000 claims description 40
- 102000036639 antigens Human genes 0.000 claims description 40
- 230000003053 immunization Effects 0.000 claims description 40
- 238000002649 immunization Methods 0.000 claims description 40
- 108010068327 4-hydroxyphenylpyruvate dioxygenase Proteins 0.000 claims description 36
- 241000283690 Bos taurus Species 0.000 claims description 36
- 101001065501 Escherichia phage MS2 Lysis protein Proteins 0.000 claims description 36
- 108091092724 Noncoding DNA Proteins 0.000 claims description 36
- 101150039660 HA gene Proteins 0.000 claims description 35
- 230000004048 modification Effects 0.000 claims description 33
- 238000012986 modification Methods 0.000 claims description 33
- 238000012217 deletion Methods 0.000 claims description 29
- 230000037430 deletion Effects 0.000 claims description 29
- 230000005847 immunogenicity Effects 0.000 claims description 28
- 230000012010 growth Effects 0.000 claims description 27
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 25
- 238000000338 in vitro Methods 0.000 claims description 24
- 241000711798 Rabies lyssavirus Species 0.000 claims description 22
- 230000005875 antibody response Effects 0.000 claims description 21
- 101150034575 piv gene Proteins 0.000 claims description 21
- 101710141454 Nucleoprotein Proteins 0.000 claims description 20
- 108010089430 Phosphoproteins Proteins 0.000 claims description 20
- 102000007982 Phosphoproteins Human genes 0.000 claims description 20
- 230000008859 change Effects 0.000 claims description 20
- 238000002255 vaccination Methods 0.000 claims description 20
- 241000725303 Human immunodeficiency virus Species 0.000 claims description 18
- 241000711386 Mumps virus Species 0.000 claims description 18
- 238000010348 incorporation Methods 0.000 claims description 18
- 230000001105 regulatory effect Effects 0.000 claims description 18
- 230000002068 genetic effect Effects 0.000 claims description 16
- 241000710929 Alphavirus Species 0.000 claims description 15
- 241000701022 Cytomegalovirus Species 0.000 claims description 15
- 241000711950 Filoviridae Species 0.000 claims description 15
- 241000710831 Flavivirus Species 0.000 claims description 15
- 241000713112 Orthobunyavirus Species 0.000 claims description 15
- 241000700584 Simplexvirus Species 0.000 claims description 15
- 108091081024 Start codon Proteins 0.000 claims description 15
- 241001493065 dsRNA viruses Species 0.000 claims description 15
- 239000013604 expression vector Substances 0.000 claims description 15
- 238000001727 in vivo Methods 0.000 claims description 15
- 230000002829 reductive effect Effects 0.000 claims description 15
- 108091006027 G proteins Proteins 0.000 claims description 14
- 102000030782 GTP binding Human genes 0.000 claims description 14
- 108091000058 GTP-Binding Proteins 0.000 claims description 14
- 241000701044 Human gammaherpesvirus 4 Species 0.000 claims description 14
- 108020004705 Codon Proteins 0.000 claims description 13
- 241000701806 Human papillomavirus Species 0.000 claims description 13
- 230000002103 transcriptional effect Effects 0.000 claims description 13
- 101710181008 P protein Proteins 0.000 claims description 12
- 101710177166 Phosphoprotein Proteins 0.000 claims description 12
- 230000001965 increasing effect Effects 0.000 claims description 12
- 101710194807 Protective antigen Proteins 0.000 claims description 11
- COLNVLDHVKWLRT-QMMMGPOBSA-N phenylalanine group Chemical group N[C@@H](CC1=CC=CC=C1)C(=O)O COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 11
- 241000712461 unidentified influenza virus Species 0.000 claims description 11
- 238000010357 RNA editing Methods 0.000 claims description 10
- 230000026279 RNA modification Effects 0.000 claims description 10
- 239000000443 aerosol Substances 0.000 claims description 10
- 230000000295 complement effect Effects 0.000 claims description 10
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims description 10
- 101150034814 F gene Proteins 0.000 claims description 9
- 101100339442 Human parainfluenza 2 virus HN gene Proteins 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 101710117545 C protein Proteins 0.000 claims description 7
- 101710085938 Matrix protein Proteins 0.000 claims description 7
- 101710127721 Membrane protein Proteins 0.000 claims description 7
- 108700005078 Synthetic Genes Proteins 0.000 claims description 7
- 210000005220 cytoplasmic tail Anatomy 0.000 claims description 7
- 231100000221 frame shift mutation induction Toxicity 0.000 claims description 7
- 230000037433 frameshift Effects 0.000 claims description 7
- 239000006166 lysate Substances 0.000 claims description 7
- 230000004044 response Effects 0.000 claims description 7
- 230000002441 reversible effect Effects 0.000 claims description 7
- 102000004127 Cytokines Human genes 0.000 claims description 6
- 108090000695 Cytokines Proteins 0.000 claims description 6
- 230000000241 respiratory effect Effects 0.000 claims description 6
- 210000000987 immune system Anatomy 0.000 claims description 5
- 108091029795 Intergenic region Proteins 0.000 claims description 4
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 4
- 101001039853 Sonchus yellow net virus Matrix protein Proteins 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 101710204837 Envelope small membrane protein Proteins 0.000 claims description 3
- 101150082239 G gene Proteins 0.000 claims description 3
- 101710128560 Initiator protein NS1 Proteins 0.000 claims description 3
- 101710145006 Lysis protein Proteins 0.000 claims description 3
- 101710144127 Non-structural protein 1 Proteins 0.000 claims description 3
- 230000004936 stimulating effect Effects 0.000 claims description 2
- 238000000917 particle-image velocimetry Methods 0.000 claims 7
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 claims 3
- 108700005307 Human papillomavirus HPV L1 Proteins 0.000 claims 2
- SFKQVVDKFKYTNA-OGNLOKFRSA-N (2S)-N-[(2R)-6-amino-1-[(2-amino-2-oxoethyl)amino]-1-oxohexan-2-yl]-1-[(4R,7S,10S,13S,16S,19R)-19-amino-7-(2-amino-2-oxoethyl)-10-(3-amino-3-oxopropyl)-13,16-dibenzyl-6,9,12,15,18-pentaoxo-1,2-dithia-5,8,11,14,17-pentazacycloicosane-4-carbonyl]pyrrolidine-2-carboxamide Chemical compound NCCCC[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](Cc2ccccc2)C(=O)N[C@@H](Cc2ccccc2)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N1)C(=O)NCC(N)=O SFKQVVDKFKYTNA-OGNLOKFRSA-N 0.000 claims 1
- 108010048209 Human Immunodeficiency Virus Proteins Proteins 0.000 claims 1
- 230000006978 adaptation Effects 0.000 claims 1
- 241000712003 Human respirovirus 3 Species 0.000 description 195
- 229960005486 vaccine Drugs 0.000 description 153
- 235000018102 proteins Nutrition 0.000 description 151
- 239000002299 complementary DNA Substances 0.000 description 91
- 230000000875 corresponding effect Effects 0.000 description 67
- 235000001014 amino acid Nutrition 0.000 description 59
- 229940125575 vaccine candidate Drugs 0.000 description 53
- 229940024606 amino acid Drugs 0.000 description 42
- 241000712005 Bovine respirovirus 3 Species 0.000 description 36
- 230000003612 virological effect Effects 0.000 description 34
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 33
- 239000013615 primer Substances 0.000 description 30
- 241000699800 Cricetinae Species 0.000 description 27
- 230000000694 effects Effects 0.000 description 27
- 238000007792 addition Methods 0.000 description 26
- 239000000047 product Substances 0.000 description 25
- 241000282412 Homo Species 0.000 description 24
- 239000013612 plasmid Substances 0.000 description 22
- 238000002703 mutagenesis Methods 0.000 description 21
- 231100000350 mutagenesis Toxicity 0.000 description 21
- 101150008820 HN gene Proteins 0.000 description 20
- 238000003757 reverse transcription PCR Methods 0.000 description 19
- 238000010276 construction Methods 0.000 description 18
- 210000002966 serum Anatomy 0.000 description 18
- 241001465754 Metazoa Species 0.000 description 17
- 108020004999 messenger RNA Proteins 0.000 description 16
- 230000036039 immunity Effects 0.000 description 15
- 229940124862 Measles virus vaccine Drugs 0.000 description 13
- 230000003472 neutralizing effect Effects 0.000 description 13
- 230000014616 translation Effects 0.000 description 13
- 238000011144 upstream manufacturing Methods 0.000 description 13
- 230000003247 decreasing effect Effects 0.000 description 12
- 238000011161 development Methods 0.000 description 12
- 238000006386 neutralization reaction Methods 0.000 description 12
- 238000004113 cell culture Methods 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 11
- 238000010367 cloning Methods 0.000 description 11
- 230000035945 sensitivity Effects 0.000 description 11
- 238000013519 translation Methods 0.000 description 11
- 108020004414 DNA Proteins 0.000 description 10
- 108091028043 Nucleic acid sequence Proteins 0.000 description 10
- 108010067390 Viral Proteins Proteins 0.000 description 10
- 238000010586 diagram Methods 0.000 description 10
- 238000009472 formulation Methods 0.000 description 10
- 108020001507 fusion proteins Proteins 0.000 description 10
- 102000037865 fusion proteins Human genes 0.000 description 10
- 101150063938 ply gene Proteins 0.000 description 10
- 108091008146 restriction endonucleases Proteins 0.000 description 10
- 238000001890 transfection Methods 0.000 description 10
- 230000001018 virulence Effects 0.000 description 10
- 108020004635 Complementary DNA Proteins 0.000 description 9
- 241001183012 Modified Vaccinia Ankara virus Species 0.000 description 9
- 108091034117 Oligonucleotide Proteins 0.000 description 9
- 241000711975 Vesicular stomatitis virus Species 0.000 description 9
- 230000001086 cytosolic effect Effects 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 9
- 229960003213 live attenuated measles Drugs 0.000 description 9
- 230000009467 reduction Effects 0.000 description 9
- 108091026890 Coding region Proteins 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 230000008707 rearrangement Effects 0.000 description 8
- 101150062031 L gene Proteins 0.000 description 7
- 101150084044 P gene Proteins 0.000 description 7
- 239000002671 adjuvant Substances 0.000 description 7
- 238000010171 animal model Methods 0.000 description 7
- 238000009739 binding Methods 0.000 description 7
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 7
- 108090000765 processed proteins & peptides Proteins 0.000 description 7
- 108020001580 protein domains Proteins 0.000 description 7
- 230000029812 viral genome replication Effects 0.000 description 7
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 6
- 230000004075 alteration Effects 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- 230000021633 leukocyte mediated immunity Effects 0.000 description 6
- 210000004072 lung Anatomy 0.000 description 6
- 230000001404 mediated effect Effects 0.000 description 6
- 238000003259 recombinant expression Methods 0.000 description 6
- 230000003362 replicative effect Effects 0.000 description 6
- 208000024891 symptom Diseases 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 210000001944 turbinate Anatomy 0.000 description 6
- 210000003501 vero cell Anatomy 0.000 description 6
- 102100031780 Endonuclease Human genes 0.000 description 5
- 101710154606 Hemagglutinin Proteins 0.000 description 5
- 241000282560 Macaca mulatta Species 0.000 description 5
- 241000711408 Murine respirovirus Species 0.000 description 5
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 5
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 5
- 101710176177 Protein A56 Proteins 0.000 description 5
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 5
- 101710137500 T7 RNA polymerase Proteins 0.000 description 5
- 241000700618 Vaccinia virus Species 0.000 description 5
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Chemical compound CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 230000004071 biological effect Effects 0.000 description 5
- 229960000074 biopharmaceutical Drugs 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 230000028996 humoral immune response Effects 0.000 description 5
- 238000013507 mapping Methods 0.000 description 5
- 230000008774 maternal effect Effects 0.000 description 5
- 229940041323 measles vaccine Drugs 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 238000002741 site-directed mutagenesis Methods 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- 108090000994 Catalytic RNA Proteins 0.000 description 4
- 102000053642 Catalytic RNA Human genes 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 206010062016 Immunosuppression Diseases 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 241001559185 Mammalian rubulavirus 5 Species 0.000 description 4
- 208000005647 Mumps Diseases 0.000 description 4
- 108010076504 Protein Sorting Signals Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 239000013592 cell lysate Substances 0.000 description 4
- 239000013553 cell monolayer Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000004927 fusion Effects 0.000 description 4
- 239000000185 hemagglutinin Substances 0.000 description 4
- 230000001506 immunosuppresive effect Effects 0.000 description 4
- 230000001976 improved effect Effects 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 208000010805 mumps infectious disease Diseases 0.000 description 4
- 230000000644 propagated effect Effects 0.000 description 4
- 108091092562 ribozyme Proteins 0.000 description 4
- 230000003248 secreting effect Effects 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 241000711404 Avian avulavirus 1 Species 0.000 description 3
- 108060003393 Granulin Proteins 0.000 description 3
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 3
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 3
- 206010061598 Immunodeficiency Diseases 0.000 description 3
- 102000008070 Interferon-gamma Human genes 0.000 description 3
- 108010074328 Interferon-gamma Proteins 0.000 description 3
- 102000003810 Interleukin-18 Human genes 0.000 description 3
- 108090000171 Interleukin-18 Proteins 0.000 description 3
- 108010002350 Interleukin-2 Proteins 0.000 description 3
- 102000000588 Interleukin-2 Human genes 0.000 description 3
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 3
- 241001529936 Murinae Species 0.000 description 3
- 206010033078 Otitis media Diseases 0.000 description 3
- 102100030852 Run domain Beclin-1-interacting and cysteine-rich domain-containing protein Human genes 0.000 description 3
- 108020005038 Terminator Codon Proteins 0.000 description 3
- 108700005077 Viral Genes Proteins 0.000 description 3
- 238000002679 ablation Methods 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- -1 cationic lipid Chemical class 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 238000001976 enzyme digestion Methods 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 230000001894 hemadsorption Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 208000030500 lower respiratory tract disease Diseases 0.000 description 3
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 3
- 244000000010 microbial pathogen Species 0.000 description 3
- 238000004264 monolayer culture Methods 0.000 description 3
- 210000001989 nasopharynx Anatomy 0.000 description 3
- 230000008506 pathogenesis Effects 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 238000010188 recombinant method Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 235000002639 sodium chloride Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 230000009885 systemic effect Effects 0.000 description 3
- 230000000699 topical effect Effects 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 244000052613 viral pathogen Species 0.000 description 3
- 230000006490 viral transcription Effects 0.000 description 3
- 210000002845 virion Anatomy 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 241000271566 Aves Species 0.000 description 2
- 206010006448 Bronchiolitis Diseases 0.000 description 2
- 241000712083 Canine morbillivirus Species 0.000 description 2
- 241000122205 Chamaeleonidae Species 0.000 description 2
- 108700010070 Codon Usage Proteins 0.000 description 2
- 108010079245 Cystic Fibrosis Transmembrane Conductance Regulator Proteins 0.000 description 2
- 102100023419 Cystic fibrosis transmembrane conductance regulator Human genes 0.000 description 2
- 108010041986 DNA Vaccines Proteins 0.000 description 2
- 229940021995 DNA vaccine Drugs 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 108700004031 HN Proteins 0.000 description 2
- 241000724709 Hepatitis delta virus Species 0.000 description 2
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 2
- 102000014150 Interferons Human genes 0.000 description 2
- 108010050904 Interferons Proteins 0.000 description 2
- 102000013462 Interleukin-12 Human genes 0.000 description 2
- 108010065805 Interleukin-12 Proteins 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 206010024971 Lower respiratory tract infections Diseases 0.000 description 2
- 241000699673 Mesocricetus auratus Species 0.000 description 2
- 108010021466 Mutant Proteins Proteins 0.000 description 2
- 102000008300 Mutant Proteins Human genes 0.000 description 2
- 102000005348 Neuraminidase Human genes 0.000 description 2
- 108010006232 Neuraminidase Proteins 0.000 description 2
- 206010035664 Pneumonia Diseases 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 208000018569 Respiratory Tract disease Diseases 0.000 description 2
- 241000711897 Rinderpest morbillivirus Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 241000144282 Sigmodon Species 0.000 description 2
- 108010008038 Synthetic Vaccines Proteins 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 2
- 206010046865 Vaccinia virus infection Diseases 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 230000027645 antigenic variation Effects 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000002962 chemical mutagen Substances 0.000 description 2
- 238000012875 competitive assay Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 201000010549 croup Diseases 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- MWRBNPKJOOWZPW-CLFAGFIQSA-N dioleoyl phosphatidylethanolamine Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(=O)OCCN)OC(=O)CCCCCCC\C=C/CCCCCCCC MWRBNPKJOOWZPW-CLFAGFIQSA-N 0.000 description 2
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 2
- 238000009510 drug design Methods 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 229940044627 gamma-interferon Drugs 0.000 description 2
- 230000004077 genetic alteration Effects 0.000 description 2
- 231100000118 genetic alteration Toxicity 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- 229960002591 hydroxyproline Drugs 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 229940079322 interferon Drugs 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229940124590 live attenuated vaccine Drugs 0.000 description 2
- 229940023012 live-attenuated vaccine Drugs 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000003471 mutagenic agent Substances 0.000 description 2
- 231100000707 mutagenic chemical Toxicity 0.000 description 2
- 230000003505 mutagenic effect Effects 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 238000012809 post-inoculation Methods 0.000 description 2
- 238000011809 primate model Methods 0.000 description 2
- 238000011321 prophylaxis Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000010814 radioimmunoprecipitation assay Methods 0.000 description 2
- 229940124551 recombinant vaccine Drugs 0.000 description 2
- 230000009711 regulatory function Effects 0.000 description 2
- 206010039083 rhinitis Diseases 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 230000000153 supplemental effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 210000001550 testis Anatomy 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 230000005030 transcription termination Effects 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 208000007089 vaccinia Diseases 0.000 description 2
- 239000004474 valine Substances 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- BEJKOYIMCGMNRB-GRHHLOCNSA-N (2s)-2-amino-3-(4-hydroxyphenyl)propanoic acid;(2s)-2-amino-3-phenylpropanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1.OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 BEJKOYIMCGMNRB-GRHHLOCNSA-N 0.000 description 1
- ICLYJLBTOGPLMC-KVVVOXFISA-N (z)-octadec-9-enoate;tris(2-hydroxyethyl)azanium Chemical compound OCCN(CCO)CCO.CCCCCCCC\C=C/CCCCCCCC(O)=O ICLYJLBTOGPLMC-KVVVOXFISA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 description 1
- 108020005345 3' Untranslated Regions Proteins 0.000 description 1
- BRMWTNUJHUMWMS-UHFFFAOYSA-N 3-Methylhistidine Natural products CN1C=NC(CC(N)C(O)=O)=C1 BRMWTNUJHUMWMS-UHFFFAOYSA-N 0.000 description 1
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- FHIDNBAQOFJWCA-UAKXSSHOSA-N 5-fluorouridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(F)=C1 FHIDNBAQOFJWCA-UAKXSSHOSA-N 0.000 description 1
- 229940117976 5-hydroxylysine Drugs 0.000 description 1
- XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Chemical compound CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001440741 CHER virus Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 108091062157 Cis-regulatory element Proteins 0.000 description 1
- 206010011416 Croup infectious Diseases 0.000 description 1
- 150000008574 D-amino acids Chemical class 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 1
- 241000701832 Enterobacteria phage T3 Species 0.000 description 1
- 241000991587 Enterovirus C Species 0.000 description 1
- 102000003951 Erythropoietin Human genes 0.000 description 1
- 108090000394 Erythropoietin Proteins 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- 108010017544 Glucosylceramidase Proteins 0.000 description 1
- 102000004547 Glucosylceramidase Human genes 0.000 description 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 229940124872 Hepatitis B virus vaccine Drugs 0.000 description 1
- 206010019799 Hepatitis viral Diseases 0.000 description 1
- 101000926057 Human herpesvirus 2 (strain G) Envelope glycoprotein C Proteins 0.000 description 1
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 description 1
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000004388 Interleukin-4 Human genes 0.000 description 1
- 108090000978 Interleukin-4 Proteins 0.000 description 1
- 108010002616 Interleukin-5 Proteins 0.000 description 1
- 102000000743 Interleukin-5 Human genes 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- 102000004889 Interleukin-6 Human genes 0.000 description 1
- 241000726306 Irus Species 0.000 description 1
- 238000012218 Kunkel's method Methods 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000701076 Macacine alphaherpesvirus 1 Species 0.000 description 1
- 241000711513 Mononegavirales Species 0.000 description 1
- 241000712045 Morbillivirus Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- DTERQYGMUDWYAZ-ZETCQYMHSA-N N(6)-acetyl-L-lysine Chemical compound CC(=O)NCCCC[C@H]([NH3+])C([O-])=O DTERQYGMUDWYAZ-ZETCQYMHSA-N 0.000 description 1
- JDHILDINMRGULE-LURJTMIESA-N N(pros)-methyl-L-histidine Chemical compound CN1C=NC=C1C[C@H](N)C(O)=O JDHILDINMRGULE-LURJTMIESA-N 0.000 description 1
- JJIHLJJYMXLCOY-BYPYZUCNSA-N N-acetyl-L-serine Chemical compound CC(=O)N[C@@H](CO)C(O)=O JJIHLJJYMXLCOY-BYPYZUCNSA-N 0.000 description 1
- PYUSHNKNPOHWEZ-YFKPBYRVSA-N N-formyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC=O PYUSHNKNPOHWEZ-YFKPBYRVSA-N 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 208000025966 Neurological disease Diseases 0.000 description 1
- 208000010359 Newcastle Disease Diseases 0.000 description 1
- 239000012124 Opti-MEM Substances 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 241000282579 Pan Species 0.000 description 1
- 241000282577 Pan troglodytes Species 0.000 description 1
- 241000711504 Paramyxoviridae Species 0.000 description 1
- 108010069013 Phenylalanine Hydroxylase Proteins 0.000 description 1
- 102100038223 Phenylalanine-4-hydroxylase Human genes 0.000 description 1
- 229940124867 Poliovirus vaccine Drugs 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 239000012083 RIPA buffer Substances 0.000 description 1
- 229940124679 RSV vaccine Drugs 0.000 description 1
- 208000036002 Rash generalised Diseases 0.000 description 1
- 101100501698 Rattus norvegicus Erbb4 gene Proteins 0.000 description 1
- 208000035415 Reinfection Diseases 0.000 description 1
- 241001068263 Replication competent viruses Species 0.000 description 1
- 208000036071 Rhinorrhea Diseases 0.000 description 1
- 206010039101 Rhinorrhoea Diseases 0.000 description 1
- 108020005148 Ribonucleic Acid Regulatory Sequences Proteins 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 241000702670 Rotavirus Species 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 241000966334 Simias Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000251131 Sphyrna Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 1
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 1
- 206010058874 Viraemia Diseases 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 108010059722 Viral Fusion Proteins Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 238000012867 alanine scanning Methods 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229940031567 attenuated vaccine Drugs 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
- 229940031416 bivalent vaccine Drugs 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229960002713 calcium chloride Drugs 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 230000002612 cardiopulmonary effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 210000003837 chick embryo Anatomy 0.000 description 1
- 230000009137 competitive binding Effects 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 208000027744 congestion Diseases 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 231100000599 cytotoxic agent Toxicity 0.000 description 1
- 239000002619 cytotoxin Substances 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- YSMODUONRAFBET-UHFFFAOYSA-N delta-DL-hydroxylysine Natural products NCC(O)CCC(N)C(O)=O YSMODUONRAFBET-UHFFFAOYSA-N 0.000 description 1
- 229960003964 deoxycholic acid Drugs 0.000 description 1
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 206010014599 encephalitis Diseases 0.000 description 1
- YSMODUONRAFBET-UHNVWZDZSA-N erythro-5-hydroxy-L-lysine Chemical compound NC[C@H](O)CC[C@H](N)C(O)=O YSMODUONRAFBET-UHNVWZDZSA-N 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 229940105423 erythropoietin Drugs 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 230000007236 host immunity Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 125000001841 imino group Chemical group [H]N=* 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 229960003130 interferon gamma Drugs 0.000 description 1
- 229940028885 interleukin-4 Drugs 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 208000017629 mild encephalitis Diseases 0.000 description 1
- 229940035032 monophosphoryl lipid a Drugs 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 239000006199 nebulizer Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 208000005923 otitis media with effusion Diseases 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 210000003681 parotid gland Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 238000002962 plaque-reduction assay Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 229960002816 potassium chloride Drugs 0.000 description 1
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 208000026425 severe pneumonia Diseases 0.000 description 1
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 229960004249 sodium acetate Drugs 0.000 description 1
- 229960002668 sodium chloride Drugs 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 229940035044 sorbitan monolaurate Drugs 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000008174 sterile solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 101150065190 term gene Proteins 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 229940117013 triethanolamine oleate Drugs 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 210000000605 viral structure Anatomy 0.000 description 1
- 229960004854 viral vaccine Drugs 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5256—Virus expressing foreign proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18641—Use of virus, viral particle or viral elements as a vector
- C12N2760/18643—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17019599P | 1999-12-10 | 1999-12-10 | |
| US09/459,062 US7250171B1 (en) | 1997-05-23 | 1999-12-10 | Construction and use of recombinant parainfluenza viruses expressing a chimeric glycoprotein |
| US09/459,062 | 1999-12-10 | ||
| US09/458,813 | 1999-12-10 | ||
| US60/170,195 | 1999-12-10 | ||
| US09/458,813 US7314631B1 (en) | 1997-05-23 | 1999-12-10 | Use of recombinant live-attenuated parainfluenza virus (PIV) as a vector to protect against disease caused by PIV and respiratory syncytial virus (RSV) |
| PCT/US2000/033293 WO2001042445A2 (en) | 1999-12-10 | 2000-12-08 | USE OF RECOMBINANT PARAINFLUENZA VIRUSES (PIVs) AS VECTORS TO PROTECT AGAINST INFECTION AND DISEASE CAUSED BY PIV AND OTHER HUMAN PATHOGENS |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2073101A AU2073101A (en) | 2001-06-18 |
| AU785148B2 true AU785148B2 (en) | 2006-10-05 |
Family
ID=27389780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU20731/01A Ceased AU785148B2 (en) | 1999-12-10 | 2000-12-08 | Use of recombinant parainfluenza viruses (PIVs) as vectors to protect against infection and disease caused by PIV and other human pathogens |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1179054A2 (https=) |
| JP (1) | JP4795597B2 (https=) |
| CN (2) | CN102766605A (https=) |
| AU (1) | AU785148B2 (https=) |
| CA (1) | CA2362685A1 (https=) |
| IL (1) | IL144831A0 (https=) |
| MX (1) | MXPA01008108A (https=) |
| WO (1) | WO2001042445A2 (https=) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6764685B1 (en) | 2000-03-21 | 2004-07-20 | Medimmune Vaccines, Inc. | Recombinant parainfluenza virus expression systems and vaccines |
| CA2435180C (en) | 2001-01-19 | 2019-04-09 | Vironovative B.V. | A virus causing respiratory tract illness in susceptible mammals |
| US8715922B2 (en) | 2001-01-19 | 2014-05-06 | ViroNovative | Virus causing respiratory tract illness in susceptible mammals |
| ATE509097T1 (de) | 2001-11-21 | 2011-05-15 | Us Health | Gewinnung von rekombinantem menschlichem parainfluenzavirus typ 1 (hpivi) aus cdna |
| CN1646684B (zh) | 2002-02-21 | 2010-10-06 | 免疫医疗疫苗公司 | 重组副流感病毒表达系统以及包含源自间质肺病毒的异种抗原的疫苗 |
| EP1539944A4 (en) | 2002-07-01 | 2005-12-28 | Univ Wayne State | METHOD AND COMPOSITIONS FOR IDENTIFYING ANTIBIOTICS HARMFUL TO ANTIBIOTIC RESISTANCE |
| WO2004027037A2 (en) | 2002-09-18 | 2004-04-01 | The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services | RECOVERY OF RECOMBINANT HUMAN PARAINFLUENZA VIRUS TYPE 2 (HPIV2) FROM cDNA AND USE OF RECOMBINANT HPIV2 IN IMMUNOGENIC COMPOSITIONS AND AS VECTORS TO ELICIT IMMUNE RESPONSES AGAINST PIV AND OTHER HUMAN PATHOGENS |
| JP2007524372A (ja) | 2003-02-28 | 2007-08-30 | ザ ガバメント オブ ザ ユナイテッド ステイツ オブ アメリカ アズ リプレゼンテッド バイ ザ セクレタリー オブ ザ デパートメント オブ ヘルス アンド ヒューマン サービシーズ | 組換えヒトメタニューモウイルスおよびその使用 |
| CN101410519B (zh) | 2003-04-25 | 2013-04-24 | 免疫医疗有限责任公司 | 间质肺病毒株及其在疫苗制剂中以及用作抗原性序列表达载体的用途和繁殖病毒的方法 |
| CN1871355B (zh) | 2003-06-09 | 2011-12-14 | 惠氏 | 从cDNA中回收非节段性负链RNA病毒的改进方法 |
| DE102005006388A1 (de) | 2005-02-11 | 2006-08-24 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Replikationsdefiziente RNA-Viren als Impfstoffe |
| AU2006243923B2 (en) | 2005-05-11 | 2011-09-22 | Wayne State University | Novel targets for the identification of antibiotics that are not susceptible to antibiotic resistance |
| US8293517B2 (en) | 2005-05-11 | 2012-10-23 | Wayne State University | Methods and compositions for the identification of antibiotics that are not susceptible to antibiotic resistance in Pseudomonas aeruginosa |
| JP4905649B2 (ja) * | 2006-03-08 | 2012-03-28 | 国立大学法人 長崎大学 | 魚用ワクチン、その製造方法、および魚類感染症の予防方法 |
| JP4892296B2 (ja) | 2006-08-24 | 2012-03-07 | 川崎製薬株式会社 | 魚類のエドワジェラ症及び連鎖球菌症用ワクチン |
| US8362203B2 (en) | 2009-02-10 | 2013-01-29 | Wayne State University | Non-natural peptides as models for the development of antibiotics |
| EP3101122B1 (en) | 2009-08-24 | 2023-06-14 | Baylor College of Medicine | Generation of ctl lines with specificity against multiple tumor antigens or multiple viruses |
| ES2728248T3 (es) * | 2012-01-24 | 2019-10-23 | Univ Georgia | Vacunas basadas en el virus paragripal 5 |
| PT2812431T (pt) | 2012-02-09 | 2019-10-18 | Baylor College Medicine | Pepmixes para gerar ctls multivirais com larga especifidade |
| CN103773803A (zh) * | 2014-01-23 | 2014-05-07 | 中国农业科学院特产研究所 | 表达猪o型口蹄疫病毒vp1蛋白的重组牛副流感病毒载体 |
| CN110996998A (zh) * | 2017-05-29 | 2020-04-10 | 美利坚合众国- 由健康及人类服务部部长代表 | 表达rsv g的重组嵌合牛/人副流感病毒3及其用途 |
| SG11202012349QA (en) * | 2018-06-27 | 2021-01-28 | Medicago Inc | Influenza virus hemagglutinin mutants |
| EP3844758A1 (en) * | 2018-08-28 | 2021-07-07 | Koninklijke Philips N.V. | Method for assessing genome alignment basis |
| IL286629B2 (en) * | 2019-03-25 | 2024-10-01 | Baylor College Medicine | Multi-respiratory virus antigen-specific t cells and methods of making and using the same therapeutically |
| CN113462656B (zh) * | 2021-03-24 | 2022-09-30 | 兰州生物制品研究所有限责任公司 | 一种人三型副流感病毒冷适应温度敏感株及其应用 |
| CN113999292B (zh) * | 2021-10-27 | 2023-05-26 | 中国农业大学 | 靶向呼吸道病毒属病毒的多肽抑制剂 |
| CA3257090A1 (en) | 2022-05-25 | 2023-11-30 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | REDUCED VECTOR ANTIGENICITY VACCINE |
| TW202535439A (zh) | 2023-11-17 | 2025-09-16 | 美商賽諾菲巴斯德公司 | 海藻糖疫苗調配物 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998053078A1 (en) * | 1997-05-23 | 1998-11-26 | The Government Of The United States Of America, As Represented By The Department Of Health And Humanservices | Production of attenuated parainfluenza virus vaccines from cloned nucleotide sequences |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101392239A (zh) * | 1999-07-09 | 2009-03-25 | 美国政府健康及人类服务部 | 减毒的人-牛嵌合副流感病毒(piv)疫苗 |
-
2000
- 2000-12-08 AU AU20731/01A patent/AU785148B2/en not_active Ceased
- 2000-12-08 CN CN201010556188XA patent/CN102766605A/zh active Pending
- 2000-12-08 CN CN00805939A patent/CN1347453A/zh active Pending
- 2000-12-08 CA CA002362685A patent/CA2362685A1/en not_active Abandoned
- 2000-12-08 JP JP2001544321A patent/JP4795597B2/ja not_active Expired - Fee Related
- 2000-12-08 WO PCT/US2000/033293 patent/WO2001042445A2/en not_active Ceased
- 2000-12-08 IL IL14483100A patent/IL144831A0/xx unknown
- 2000-12-08 MX MXPA01008108A patent/MXPA01008108A/es not_active Application Discontinuation
- 2000-12-08 EP EP00984052A patent/EP1179054A2/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998053078A1 (en) * | 1997-05-23 | 1998-11-26 | The Government Of The United States Of America, As Represented By The Department Of Health And Humanservices | Production of attenuated parainfluenza virus vaccines from cloned nucleotide sequences |
Non-Patent Citations (1)
| Title |
|---|
| SKIADOPOULOS, M ETAL (1999) VACCINE 18(5-6): 503-510 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2362685A1 (en) | 2001-06-14 |
| IL144831A0 (en) | 2002-06-30 |
| MXPA01008108A (es) | 2004-11-12 |
| CN1347453A (zh) | 2002-05-01 |
| WO2001042445A2 (en) | 2001-06-14 |
| CN102766605A (zh) | 2012-11-07 |
| WO2001042445A3 (en) | 2001-11-29 |
| JP4795597B2 (ja) | 2011-10-19 |
| JP2003516148A (ja) | 2003-05-13 |
| AU2073101A (en) | 2001-06-18 |
| EP1179054A2 (en) | 2002-02-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU785148B2 (en) | Use of recombinant parainfluenza viruses (PIVs) as vectors to protect against infection and disease caused by PIV and other human pathogens | |
| US7192593B2 (en) | Use of recombinant parainfluenza viruses (PIVs) as vectors to protect against infection and disease caused by PIV and other human pathogens | |
| US7951383B2 (en) | Attenuated parainfluenza virus (PIV) vaccines | |
| JP4237268B2 (ja) | クローン化されたヌクレオチド配列からの弱毒化パラインフルエンザウィルスワクチンの製造 | |
| AU2009202359A1 (en) | Recovery of recombinant human parainfluenza virus type 1 (HPIV1) from cDNA use of recombinant HPIV1 in immunogenic compositions and as vectors to elicit immune responses against PIV and other human pathogens | |
| AU783993C (en) | Recombinant parainfluenza virus vaccines attenuated by deletion or ablation of a non-essential gene | |
| AU2003270824B2 (en) | Recovery of recombinant human parainfluenza virus type 2 (HPIV2) from cDNA and use of recombinant HPIV2 in immunogenic compositions and as vectors to elicit immune responses against PIV and other human pathogens | |
| JP4813711B2 (ja) | 弱毒ヒト−ウシキメラ−パラインフルエンザウイルス（ｐｉｖ）ワクチン | |
| US7208161B1 (en) | Production of attenuated parainfluenza virus vaccines from cloned nucleotide sequences | |
| US7250171B1 (en) | Construction and use of recombinant parainfluenza viruses expressing a chimeric glycoprotein | |
| US7622123B2 (en) | Attenuated human-bovine chimeric parainfluenza virus (PIV) vaccines | |
| US7632508B2 (en) | Attenuated human-bovine chimeric parainfluenza virus (PIV) vaccines | |
| US7314631B1 (en) | Use of recombinant live-attenuated parainfluenza virus (PIV) as a vector to protect against disease caused by PIV and respiratory syncytial virus (RSV) | |
| AU783189B2 (en) | Attenuated human-bovine chimeric parainfluenza virus (PIV) vaccines |