AU775533B2 - Electropolymerizable film, and method of making and use thereof - Google Patents
Electropolymerizable film, and method of making and use thereof Download PDFInfo
- Publication number
- AU775533B2 AU775533B2 AU29835/00A AU2983500A AU775533B2 AU 775533 B2 AU775533 B2 AU 775533B2 AU 29835/00 A AU29835/00 A AU 29835/00A AU 2983500 A AU2983500 A AU 2983500A AU 775533 B2 AU775533 B2 AU 775533B2
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- Australia
- Prior art keywords
- nucleic acid
- film
- electrode
- vbpy
- oxidation
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- Ceased
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- 238000004519 manufacturing process Methods 0.000 title description 4
- 150000007523 nucleic acids Chemical class 0.000 claims description 75
- 108020004707 nucleic acids Proteins 0.000 claims description 74
- 102000039446 nucleic acids Human genes 0.000 claims description 74
- 239000010408 film Substances 0.000 claims description 57
- 239000000523 sample Substances 0.000 claims description 46
- 238000000034 method Methods 0.000 claims description 42
- 108020004414 DNA Proteins 0.000 claims description 37
- 230000003647 oxidation Effects 0.000 claims description 27
- 238000007254 oxidation reaction Methods 0.000 claims description 27
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims description 26
- 239000002751 oligonucleotide probe Substances 0.000 claims description 26
- 238000006479 redox reaction Methods 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 16
- 238000007112 amidation reaction Methods 0.000 claims description 12
- 230000009435 amidation Effects 0.000 claims description 11
- 229920001577 copolymer Polymers 0.000 claims description 8
- 150000007942 carboxylates Chemical group 0.000 claims description 7
- 238000000835 electrochemical detection Methods 0.000 claims description 7
- IRQWEODKXLDORP-UHFFFAOYSA-N 4-ethenylbenzoic acid Chemical compound OC(=O)C1=CC=C(C=C)C=C1 IRQWEODKXLDORP-UHFFFAOYSA-N 0.000 claims description 6
- 102000053602 DNA Human genes 0.000 claims description 6
- 150000001718 carbodiimides Chemical class 0.000 claims description 6
- 150000004696 coordination complex Chemical class 0.000 claims description 6
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 6
- 239000003999 initiator Substances 0.000 claims description 5
- 239000010409 thin film Substances 0.000 claims description 5
- IWZAEAAZXRSXPX-UHFFFAOYSA-N 2-(4-ethenylpyridin-2-yl)-4-methylpyridine Chemical group CC1=CC=NC(C=2N=CC=C(C=C)C=2)=C1 IWZAEAAZXRSXPX-UHFFFAOYSA-N 0.000 claims description 4
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 4
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- 239000000243 solution Substances 0.000 description 32
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- 108020003215 DNA Probes Proteins 0.000 description 11
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- VDZOOKBUILJEDG-UHFFFAOYSA-M tetrabutylammonium hydroxide Chemical compound [OH-].CCCC[N+](CCCC)(CCCC)CCCC VDZOOKBUILJEDG-UHFFFAOYSA-M 0.000 description 7
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- RGKBRPAAQSHTED-UHFFFAOYSA-N 8-oxoadenine Chemical compound NC1=NC=NC2=C1NC(=O)N2 RGKBRPAAQSHTED-UHFFFAOYSA-N 0.000 description 5
- 229910019142 PO4 Inorganic materials 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 229910021397 glassy carbon Inorganic materials 0.000 description 5
- 238000006116 polymerization reaction Methods 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- UBKVUFQGVWHZIR-UHFFFAOYSA-N 8-oxoguanine Chemical compound O=C1NC(N)=NC2=NC(=O)N=C21 UBKVUFQGVWHZIR-UHFFFAOYSA-N 0.000 description 4
- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 description 4
- 229910021607 Silver chloride Inorganic materials 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
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- 230000000295 complement effect Effects 0.000 description 4
- 229940104302 cytosine Drugs 0.000 description 4
- KTWOOEGAPBSYNW-UHFFFAOYSA-N ferrocene Chemical class [Fe+2].C=1C=C[CH-]C=1.C=1C=C[CH-]C=1 KTWOOEGAPBSYNW-UHFFFAOYSA-N 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
- 239000010452 phosphate Substances 0.000 description 4
- 235000021317 phosphate Nutrition 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Substances [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 230000027756 respiratory electron transport chain Effects 0.000 description 4
- 229910052707 ruthenium Inorganic materials 0.000 description 4
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 4
- WYWHKKSPHMUBEB-UHFFFAOYSA-N tioguanine Chemical compound N1C(N)=NC(=S)C2=C1N=CN2 WYWHKKSPHMUBEB-UHFFFAOYSA-N 0.000 description 4
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 3
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 3
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- 101710134784 Agnoprotein Proteins 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical group ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
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- PTJWIQPHWPFNBW-UHFFFAOYSA-N Pseudouridine C Natural products OC1C(O)C(CO)OC1C1=CNC(=O)NC1=O PTJWIQPHWPFNBW-UHFFFAOYSA-N 0.000 description 2
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical group C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 2
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
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- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10S435/817—Enzyme or microbe electrode
Landscapes
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- Engineering & Computer Science (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/267552 | 1999-03-12 | ||
| US09/267,552 US6180346B1 (en) | 1995-06-27 | 1999-03-12 | Electropolymerizable film, and method of making and use thereof |
| PCT/US2000/003110 WO2000055366A1 (en) | 1999-03-12 | 2000-02-04 | Electropolymerizable film, and method of making and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2983500A AU2983500A (en) | 2000-10-04 |
| AU775533B2 true AU775533B2 (en) | 2004-08-05 |
Family
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Family Applications (1)
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|---|---|---|---|
| AU29835/00A Ceased AU775533B2 (en) | 1999-03-12 | 2000-02-04 | Electropolymerizable film, and method of making and use thereof |
Country Status (11)
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|---|---|
| US (1) | US6180346B1 (enExample) |
| EP (1) | EP1161560A1 (enExample) |
| JP (1) | JP2002539462A (enExample) |
| KR (1) | KR20010104724A (enExample) |
| CN (1) | CN1165629C (enExample) |
| AU (1) | AU775533B2 (enExample) |
| CA (1) | CA2366866A1 (enExample) |
| HK (1) | HK1044024A1 (enExample) |
| NO (1) | NO20014429L (enExample) |
| NZ (1) | NZ513604A (enExample) |
| WO (1) | WO2000055366A1 (enExample) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6833267B1 (en) * | 1998-12-30 | 2004-12-21 | Clinical Micro Sensors, Inc. | Tissue collection devices containing biosensors |
| EP1134294A3 (en) * | 2000-03-16 | 2004-06-30 | Kabushiki Kaisha Toshiba | Method for producing immobilized nucleic acid strand |
| US20020155476A1 (en) * | 2000-10-20 | 2002-10-24 | Nader Pourmand | Transient electrical signal based methods and devices for characterizing molecular interaction and/or motion in a sample |
| DE10126798A1 (de) | 2001-06-01 | 2002-12-19 | Nanotype Gmbh | Verfahren zur Bestimmung einer Probe |
| GB0205455D0 (en) | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
| WO2004023128A1 (en) * | 2002-09-05 | 2004-03-18 | Unisearch Limited | Detection of target nucleic acid molecules by alteration of reaction of a redox species following hybridization with immoblized capture nucleic acid molecules |
| US7258978B2 (en) * | 2002-11-06 | 2007-08-21 | Geneohm Sciences | Electrochemical method to measure DNA attachment to an electrode surface in the presence of molecular oxygen |
| US20040086892A1 (en) * | 2002-11-06 | 2004-05-06 | Crothers Donald M. | Universal tag assay |
| DE10253337B4 (de) * | 2002-11-14 | 2005-10-20 | November Ag Molekulare Medizin | Verfahren zum Nachweis einer Nukleinsäure |
| US7138121B2 (en) * | 2003-01-23 | 2006-11-21 | Spangler Brenda D | Biosensors utilizing dendrimer-immobilized ligands and there use thereof |
| JP4666525B2 (ja) * | 2003-05-02 | 2011-04-06 | ジーンオーム サイエンシーズ、インク． | 電極表面に対するｄｎａ結合を分子酸素の存在下で測定するための電気化学的方法 |
| WO2004096824A2 (en) * | 2003-05-02 | 2004-11-11 | Centrum Mikrobiologii I Wirusologii Polskiej Akademii Nauk | Nucleoside derivative, modified oligonucleotide, method for their synthesis and applications thereof |
| US7741033B2 (en) * | 2003-05-13 | 2010-06-22 | Trustees Of Boston College | Electrocatalytic nucleic acid hybridization detection |
| AU2004299864B2 (en) * | 2003-12-15 | 2010-02-04 | Geneohm Sciences, Inc. | Carbon electrode surface for attachment of DNA and protein molecules |
| JP3952042B2 (ja) * | 2004-06-07 | 2007-08-01 | ソニー株式会社 | 凹状部位を有する電極を備えるハイブリダイゼーション検出部と該検出部を備えるｄｎａチップ |
| US20060094024A1 (en) * | 2004-11-01 | 2006-05-04 | Pirrung Michael C | Electrochemical arrays |
| CA2604471A1 (en) * | 2005-04-12 | 2007-08-23 | The Trustees Of Boston College | Method for electrocatalytic protein detection |
| WO2010025547A1 (en) * | 2008-09-02 | 2010-03-11 | The Governing Council Of The University Of Toronto | Nanostructured microelectrodes and biosensing devices incorporating the same |
| JP5989668B2 (ja) | 2011-01-11 | 2016-09-07 | ザ ガバニング カウンシル オブ ザ ユニバーシティ オブ トロント | タンパク質検出方法 |
| ES2799422T3 (es) | 2011-03-10 | 2020-12-17 | General Atomics | Dispositivos y métodos de diagnóstico y preparación de muestras |
| EP4659246A1 (en) * | 2023-02-01 | 2025-12-10 | Atlas Data Storage, Inc. | Electrochemical synthesis with redox stable nucleotides |
| EP4596563A3 (en) * | 2024-01-31 | 2025-11-12 | i-Sens, Inc. | Electrochemical biosensor comprising transition metal complex or oxidation-reduction polymer |
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| WO1997001646A2 (en) * | 1995-06-27 | 1997-01-16 | The University Of North Carolina At Chapel Hill | Electrochemical detection of nucleic acid hybridization |
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-
1999
- 1999-03-12 US US09/267,552 patent/US6180346B1/en not_active Expired - Lifetime
-
2000
- 2000-02-04 KR KR1020017011551A patent/KR20010104724A/ko not_active Ceased
- 2000-02-04 CA CA002366866A patent/CA2366866A1/en not_active Abandoned
- 2000-02-04 CN CNB008047057A patent/CN1165629C/zh not_active Expired - Fee Related
- 2000-02-04 EP EP00908508A patent/EP1161560A1/en not_active Withdrawn
- 2000-02-04 JP JP2000605782A patent/JP2002539462A/ja not_active Withdrawn
- 2000-02-04 NZ NZ513604A patent/NZ513604A/xx unknown
- 2000-02-04 HK HK02103719.2A patent/HK1044024A1/zh unknown
- 2000-02-04 WO PCT/US2000/003110 patent/WO2000055366A1/en not_active Ceased
- 2000-02-04 AU AU29835/00A patent/AU775533B2/en not_active Ceased
-
2001
- 2001-09-12 NO NO20014429A patent/NO20014429L/no unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997001646A2 (en) * | 1995-06-27 | 1997-01-16 | The University Of North Carolina At Chapel Hill | Electrochemical detection of nucleic acid hybridization |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1343260A (zh) | 2002-04-03 |
| JP2002539462A (ja) | 2002-11-19 |
| US6180346B1 (en) | 2001-01-30 |
| NZ513604A (en) | 2003-03-28 |
| AU2983500A (en) | 2000-10-04 |
| NO20014429D0 (no) | 2001-09-12 |
| CA2366866A1 (en) | 2000-09-21 |
| KR20010104724A (ko) | 2001-11-26 |
| EP1161560A1 (en) | 2001-12-12 |
| NO20014429L (no) | 2001-11-09 |
| HK1044024A1 (zh) | 2002-10-04 |
| WO2000055366A1 (en) | 2000-09-21 |
| CN1165629C (zh) | 2004-09-08 |
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